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Rattus norvegicus aspartyl-tRNA synthetase 2 (mitochondrial) (Dars2), transcript variant 1, mRNA; nuclear gene for mitochondrial product

NCBI Reference Sequence: NM_001034143.2

FASTA Graphics 

LOCUS       NM_001034143            2686 bp    mRNA    linear   ROD 03-MAY-2024
DEFINITION  Rattus norvegicus aspartyl-tRNA synthetase 2 (mitochondrial)
            (Dars2), transcript variant 1, mRNA; nuclear gene for mitochondrial
            product.
ACCESSION   NM_001034143 XM_222804
VERSION     NM_001034143.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Rattus norvegicus (Norway rat)
  ORGANISM  Rattus norvegicus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Rattus.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Neuenfeldt,A., Lorber,B., Ennifar,E., Gaudry,A., Sauter,C.,
            Sissler,M. and Florentz,C.
  TITLE     Thermodynamic properties distinguish human mitochondrial
            aspartyl-tRNA synthetase from bacterial homolog with same 3D
            architecture
  JOURNAL   Nucleic Acids Res 41 (4), 2698-2708 (2013)
   PUBMED   23275545
REFERENCE   2  (bases 1 to 2686)
  AUTHORS   Scheper,G.C., van der Klok,T., van Andel,R.J., van Berkel,C.G.,
            Sissler,M., Smet,J., Muravina,T.I., Serkov,S.V., Uziel,G.,
            Bugiani,M., Schiffmann,R., Krageloh-Mann,I., Smeitink,J.A.,
            Florentz,C., Van Coster,R., Pronk,J.C. and van der Knaap,M.S.
  TITLE     Mitochondrial aspartyl-tRNA synthetase deficiency causes
            leukoencephalopathy with brain stem and spinal cord involvement and
            lactate elevation
  JOURNAL   Nat Genet 39 (4), 534-539 (2007)
   PUBMED   17384640
REFERENCE   3  (bases 1 to 2686)
  AUTHORS   Bonnefond,L., Fender,A., Rudinger-Thirion,J., Giege,R., Florentz,C.
            and Sissler,M.
  TITLE     Toward the full set of human mitochondrial aminoacyl-tRNA
            synthetases: characterization of AspRS and TyrRS
  JOURNAL   Biochemistry 44 (12), 4805-4816 (2005)
   PUBMED   15779907
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            JAXUCZ010000013.1.
            
            On May 3, 2024 this sequence version replaced NM_001034143.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC105774.1, SRR22582416.1247202.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA5760383,
                                           SAMEA5760386 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-634               JAXUCZ010000013.1  75869674-75870307   c
            635-734             JAXUCZ010000013.1  75868745-75868844   c
            735-801             JAXUCZ010000013.1  75865156-75865222   c
            802-903             JAXUCZ010000013.1  75863540-75863641   c
            904-999             JAXUCZ010000013.1  75863051-75863146   c
            1000-1123           JAXUCZ010000013.1  75860316-75860439   c
            1124-1170           JAXUCZ010000013.1  75859645-75859691   c
            1171-1277           JAXUCZ010000013.1  75857411-75857517   c
            1278-1347           JAXUCZ010000013.1  75856853-75856922   c
            1348-1527           JAXUCZ010000013.1  75854164-75854343   c
            1528-1635           JAXUCZ010000013.1  75851413-75851520   c
            1636-1698           JAXUCZ010000013.1  75850968-75851030   c
            1699-1851           JAXUCZ010000013.1  75847925-75848077   c
            1852-2070           JAXUCZ010000013.1  75845527-75845745   c
            2071-2181           JAXUCZ010000013.1  75845236-75845346   c
            2182-2257           JAXUCZ010000013.1  75844232-75844307   c
            2258-2686           JAXUCZ010000013.1  75842078-75842506   c
FEATURES             Location/Qualifiers
     source          1..2686
                     /organism="Rattus norvegicus"
                     /mol_type="mRNA"
                     /strain="BN"
                     /db_xref="taxon:10116"
                     /chromosome="13"
                     /map="13q22"
     gene            1..2686
                     /gene="Dars2"
                     /gene_synonym="RGD1308286"
                     /note="aspartyl-tRNA synthetase 2 (mitochondrial)"
                     /db_xref="GeneID:304919"
                     /db_xref="RGD:1308286"
     exon            1..634
                     /gene="Dars2"
                     /gene_synonym="RGD1308286"
                     /inference="alignment:Splign:2.1.0"
     misc_feature    469..471
                     /gene="Dars2"
                     /gene_synonym="RGD1308286"
                     /note="upstream in-frame stop codon"
     CDS             511..2469
                     /gene="Dars2"
                     /gene_synonym="RGD1308286"
                     /EC_number="6.1.1.12"
                     /note="isoform 1 is encoded by transcript variant 1;
                     aspartyl-tRNA synthetase, mitochondrial; aspRS;
                     aspartate--tRNA ligase, mitochondrial"
                     /codon_start=1
                     /product="aspartate--tRNA ligase, mitochondrial isoform 1"
                     /protein_id="NP_001029315.1"
                     /db_xref="GeneID:304919"
                     /db_xref="RGD:1308286"
                     /translation="MYLGSWLNRLGRGLSRPIGKTKQPIWGSLSRSLTLSSQRVPEFS
                     SFVARTNTCGELRSSHLGQEVTLCGWIQYRRQNTFLVLRDCHGLVQILIPQDESAASV
                     RRTLCEAPVESVVRVSGTVIARPLGQENPKMPTGEIEIKAKTAELLNACKKLPFEIKD
                     FVKKTEALRLQYRYLDLRSSQMQHNLRLRSQMVMKMREYLCTLHGFVDIETPTLFKRT
                     PGGAKEFLVPSREPGRFYSLPQSPQQFKQLLMVGGLDRYFQVARCYRDEGSRPDRQPE
                     FTQIDIEMSFVDQTGIQHLVEGLLHYSWPEDKDPLVAPFPSMTFAEALATYGTDKPDT
                     RFGMKIVDISDVFRNTEIRFLQDALAKPQGTVKAICVHEGAKYLRKEDIEFIRKFAAH
                     HFSQEVLPIFLNARKNWSSPFAKFITEEERLELTRLMEIQEDDMVLLTAGQHEKACSL
                     LGKLRLECADLLETRGLALRDPALFSFLWVLDFPLFLAKEESPTELESAHHPFTAPHP
                     GDIHLLYTEPEKVRGQHYDLVLNGNEIGGGSIRIHDAQLQRYILETLLKEDVKLLSHL
                     LQALDYGAPPHGGIALGLDRLVCLVTGAPSIRDVIAFPKSYRGHDLMSNAPDTVSPED
                     LKPYHIHVSWPTDSEERASATPSKYLSS"
     transit_peptide 511..648
                     /gene="Dars2"
                     /gene_synonym="RGD1308286"
                     /note="Mitochondrion. /evidence=ECO:0000255; propagated
                     from UniProtKB/Swiss-Prot (Q3KRD0.1)"
     mat_peptide     649..2466
                     /gene="Dars2"
                     /gene_synonym="RGD1308286"
                     /product="Aspartate--tRNA ligase, mitochondrial.
                     /id=PRO_0000250738"
                     /note="propagated from UniProtKB/Swiss-Prot (Q3KRD0.1)"
     misc_feature    1162..1164
                     /gene="Dars2"
                     /gene_synonym="RGD1308286"
                     /note="Phosphothreonine.
                     /evidence=ECO:0007744|PubMed:16641100; propagated from
                     UniProtKB/Swiss-Prot (Q3KRD0.1); phosphorylation site"
     misc_feature    1231..1233
                     /gene="Dars2"
                     /gene_synonym="RGD1308286"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q6PI48; propagated from
                     UniProtKB/Swiss-Prot (Q3KRD0.1); phosphorylation site"
     misc_feature    1237..1248
                     /gene="Dars2"
                     /gene_synonym="RGD1308286"
                     /note="propagated from UniProtKB/Swiss-Prot (Q3KRD0.1);
                     Region: Aspartate. /evidence=ECO:0000250"
     exon            635..734
                     /gene="Dars2"
                     /gene_synonym="RGD1308286"
                     /inference="alignment:Splign:2.1.0"
     exon            735..801
                     /gene="Dars2"
                     /gene_synonym="RGD1308286"
                     /inference="alignment:Splign:2.1.0"
     exon            802..903
                     /gene="Dars2"
                     /gene_synonym="RGD1308286"
                     /inference="alignment:Splign:2.1.0"
     exon            904..999
                     /gene="Dars2"
                     /gene_synonym="RGD1308286"
                     /inference="alignment:Splign:2.1.0"
     exon            1000..1123
                     /gene="Dars2"
                     /gene_synonym="RGD1308286"
                     /inference="alignment:Splign:2.1.0"
     exon            1124..1170
                     /gene="Dars2"
                     /gene_synonym="RGD1308286"
                     /inference="alignment:Splign:2.1.0"
     exon            1171..1277
                     /gene="Dars2"
                     /gene_synonym="RGD1308286"
                     /inference="alignment:Splign:2.1.0"
     exon            1278..1347
                     /gene="Dars2"
                     /gene_synonym="RGD1308286"
                     /inference="alignment:Splign:2.1.0"
     exon            1348..1527
                     /gene="Dars2"
                     /gene_synonym="RGD1308286"
                     /inference="alignment:Splign:2.1.0"
     exon            1528..1635
                     /gene="Dars2"
                     /gene_synonym="RGD1308286"
                     /inference="alignment:Splign:2.1.0"
     exon            1636..1698
                     /gene="Dars2"
                     /gene_synonym="RGD1308286"
                     /inference="alignment:Splign:2.1.0"
     exon            1699..1851
                     /gene="Dars2"
                     /gene_synonym="RGD1308286"
                     /inference="alignment:Splign:2.1.0"
     exon            1852..2070
                     /gene="Dars2"
                     /gene_synonym="RGD1308286"
                     /inference="alignment:Splign:2.1.0"
     exon            2071..2181
                     /gene="Dars2"
                     /gene_synonym="RGD1308286"
                     /inference="alignment:Splign:2.1.0"
     exon            2182..2257
                     /gene="Dars2"
                     /gene_synonym="RGD1308286"
                     /inference="alignment:Splign:2.1.0"
     exon            2258..2686
                     /gene="Dars2"
                     /gene_synonym="RGD1308286"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 agagggacgg gcttctggat gtgatttgtg cctgggttcg aacctcagag ttctttggat
       61 ttgctcgctt tcacctttca gaaaaattca agatgggccg ccaggtttgg gagagttgaa
      121 acacgaggct gtacctcctt atgattctgc ttatgccccg gtcaccttcg aggaacgtgg
      181 gaataaaggg aggacgtgcg cctggtgact ggccttccgg ctggggtcgc tctgatgctg
      241 gcgctgctgt ttgggatctt ttggtatctt cggggcgcag ccttgtttaa ttaaagtggg
      301 aactgatgca ctgaaggaag gcttgcccta cccactcatc cacgggatct agagaccttg
      361 cagatcaatc tatttgtaga tacttgaacg ttgtgcggag gagggcctgg atgcatttgc
      421 gtgggaagta gaattttaag ggttccctgg tattccaaag ccgagttttg agcaccacca
      481 cacctgcagc gcggattccg ggacttcaac atgtatcttg gttcttggtt aaatcgtcta
      541 ggcaggggtt tatccagacc catcggaaag accaagcagc caatctgggg ttctctttcc
      601 agaagtctga cgttgagttc acagagagtt ccagaattca gtagctttgt tgctcggacc
      661 aacacatgtg gagaacttcg ttcttcccac ttaggccaag aagtcactct gtgtggatgg
      721 attcagtacc gaaggcaaaa caccttctta gtcctaagag attgccacgg tctcgttcaa
      781 atcctcattc cccaggatga gtcggctgca tctgtgagga ggactttgtg tgaagcccct
      841 gtggagtctg tggtgagagt atctgggacg gtcatcgccc gacccctggg acaggagaat
      901 ccgaaaatgc caacaggtga gattgaaatc aaagctaaaa cagctgagct cctgaatgcc
      961 tgcaaaaagc tgccctttga aattaaagac tttgtgaaga agacggaagc ccttcgtctg
     1021 cagtatcggt acttagacct gcgcagctcc caaatgcagc acaacctgcg gctcaggtca
     1081 cagatggtca tgaaaatgcg ggaatatctc tgtactctgc acggatttgt ggatatagaa
     1141 accccaacat tgtttaagag gactcctgga ggtgccaagg agtttttagt accgtccagg
     1201 gaacctggaa ggttttattc tctccctcag agtcctcaac agtttaagca gcttctgatg
     1261 gttggtggtt tagacaggta ctttcaggtt gcccggtgtt atcgagatga aggctcaaga
     1321 ccggataggc agccagagtt tactcagatt gacatagaga tgtcctttgt agaccagact
     1381 ggcattcagc atttagttga gggtttgctg cattattcct ggcctgaaga taaagaccct
     1441 ctagtggctc cttttccttc catgactttt gctgaggcat tggctaccta cggaactgat
     1501 aagccagata ctcgctttgg gatgaagatt gtggatatca gtgatgtgtt cagaaacaca
     1561 gagatcaggt ttcttcagga tgcactggct aagccccaag gaactgtcaa agcaatatgt
     1621 gttcatgaag gagcaaagta cttaaggaaa gaagacattg agttcattcg aaagtttgca
     1681 gctcaccatt tcagtcagga agtccttcct atattcctga atgccaggaa gaactggagt
     1741 tcaccatttg ctaaattcat aacggaggag gaaagattgg agttaaccag attgatggag
     1801 atacaagaag acgacatggt gctgctgact gctgggcaac atgagaaagc atgctctttg
     1861 ttggggaagt tacgactaga gtgtgcagac cttttggaaa cgagaggact ggcgctcagg
     1921 gacccagcat tgttctcttt tctctgggta cttgacttcc cactcttcct cgccaaggaa
     1981 gagagtccca cagagctgga gtcagcccac cacccattta ctgctcctca ccccggcgac
     2041 attcacctcc tctacactga gccggaaaag gttcgtggcc aacactacga cttggtttta
     2101 aatggcaatg agataggagg aggctctatc cgcattcatg atgcacagct acagaggtac
     2161 atcctggaaa cattgctaaa ggaagatgtg aaattgcttt cccatctgct tcaggcttta
     2221 gactatgggg cacccccaca tggaggaatt gccttagggt tagacagact ggtgtgtctt
     2281 gtcacgggag cacccagcat cagagatgtc atagccttcc ctaaatccta ccgaggacat
     2341 gaccttatga gcaatgctcc agacactgtg tctcctgaag atctgaagcc ttaccatatc
     2401 cacgtctcat ggccaacaga ctcagaagag agagcatccg ccacaccttc caaatacttg
     2461 agctcttgac ttgtcttctt tgctttacta aacctaaaaa gaagatccag acttgccaca
     2521 gactttagaa tcaagtttct gtgtagaagc aatcattacc gagtaagaaa cagaagctac
     2581 acaattgtgg tcatatgtac cattaggact ttctgtagtt gggagttctg tggaagtttt
     2641 ttaattggag aattgtaaag tgaaacaaaa aaagcttttt attgga
//
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