LOCUS NM_001034143 2686 bp mRNA linear ROD 03-MAY-2024
DEFINITION Rattus norvegicus aspartyl-tRNA synthetase 2 (mitochondrial)
(Dars2), transcript variant 1, mRNA; nuclear gene for mitochondrial
product.
ACCESSION NM_001034143 XM_222804
VERSION NM_001034143.2
KEYWORDS RefSeq; RefSeq Select.
SOURCE Rattus norvegicus (Norway rat)
ORGANISM Rattus norvegicus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Rattus.
REFERENCE 1 (bases 1 to 2686)
AUTHORS Neuenfeldt,A., Lorber,B., Ennifar,E., Gaudry,A., Sauter,C.,
Sissler,M. and Florentz,C.
TITLE Thermodynamic properties distinguish human mitochondrial
aspartyl-tRNA synthetase from bacterial homolog with same 3D
architecture
JOURNAL Nucleic Acids Res 41 (4), 2698-2708 (2013)
PUBMED 23275545
REFERENCE 2 (bases 1 to 2686)
AUTHORS Scheper,G.C., van der Klok,T., van Andel,R.J., van Berkel,C.G.,
Sissler,M., Smet,J., Muravina,T.I., Serkov,S.V., Uziel,G.,
Bugiani,M., Schiffmann,R., Krageloh-Mann,I., Smeitink,J.A.,
Florentz,C., Van Coster,R., Pronk,J.C. and van der Knaap,M.S.
TITLE Mitochondrial aspartyl-tRNA synthetase deficiency causes
leukoencephalopathy with brain stem and spinal cord involvement and
lactate elevation
JOURNAL Nat Genet 39 (4), 534-539 (2007)
PUBMED 17384640
REFERENCE 3 (bases 1 to 2686)
AUTHORS Bonnefond,L., Fender,A., Rudinger-Thirion,J., Giege,R., Florentz,C.
and Sissler,M.
TITLE Toward the full set of human mitochondrial aminoacyl-tRNA
synthetases: characterization of AspRS and TyrRS
JOURNAL Biochemistry 44 (12), 4805-4816 (2005)
PUBMED 15779907
COMMENT VALIDATED REFSEQ: This record has undergone validation or
preliminary review. The reference sequence was derived from
JAXUCZ010000013.1.
On May 3, 2024 this sequence version replaced NM_001034143.1.
##Evidence-Data-START##
Transcript exon combination :: BC105774.1, SRR22582416.1247202.1
[ECO:0000332]
RNAseq introns :: mixed sample support SAMEA5760383,
SAMEA5760386 [ECO:0006172]
##Evidence-Data-END##
##RefSeq-Attributes-START##
gene product(s) localized to mito. :: inferred from homology
RefSeq Select criteria :: based on conservation,
expression, longest protein
##RefSeq-Attributes-END##
PRIMARY REFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP
1-634 JAXUCZ010000013.1 75869674-75870307 c
635-734 JAXUCZ010000013.1 75868745-75868844 c
735-801 JAXUCZ010000013.1 75865156-75865222 c
802-903 JAXUCZ010000013.1 75863540-75863641 c
904-999 JAXUCZ010000013.1 75863051-75863146 c
1000-1123 JAXUCZ010000013.1 75860316-75860439 c
1124-1170 JAXUCZ010000013.1 75859645-75859691 c
1171-1277 JAXUCZ010000013.1 75857411-75857517 c
1278-1347 JAXUCZ010000013.1 75856853-75856922 c
1348-1527 JAXUCZ010000013.1 75854164-75854343 c
1528-1635 JAXUCZ010000013.1 75851413-75851520 c
1636-1698 JAXUCZ010000013.1 75850968-75851030 c
1699-1851 JAXUCZ010000013.1 75847925-75848077 c
1852-2070 JAXUCZ010000013.1 75845527-75845745 c
2071-2181 JAXUCZ010000013.1 75845236-75845346 c
2182-2257 JAXUCZ010000013.1 75844232-75844307 c
2258-2686 JAXUCZ010000013.1 75842078-75842506 c
FEATURES Location/Qualifiers
source 1..2686
/organism="Rattus norvegicus"
/mol_type="mRNA"
/strain="BN"
/db_xref="taxon:10116"
/chromosome="13"
/map="13q22"
gene 1..2686
/gene="Dars2"
/gene_synonym="RGD1308286"
/note="aspartyl-tRNA synthetase 2 (mitochondrial)"
/db_xref="GeneID:304919"
/db_xref="RGD:1308286"
exon 1..634
/gene="Dars2"
/gene_synonym="RGD1308286"
/inference="alignment:Splign:2.1.0"
misc_feature 469..471
/gene="Dars2"
/gene_synonym="RGD1308286"
/note="upstream in-frame stop codon"
CDS 511..2469
/gene="Dars2"
/gene_synonym="RGD1308286"
/EC_number="6.1.1.12"
/note="isoform 1 is encoded by transcript variant 1;
aspartyl-tRNA synthetase, mitochondrial; aspRS;
aspartate--tRNA ligase, mitochondrial"
/codon_start=1
/product="aspartate--tRNA ligase, mitochondrial isoform 1"
/protein_id="NP_001029315.1"
/db_xref="GeneID:304919"
/db_xref="RGD:1308286"
/translation="MYLGSWLNRLGRGLSRPIGKTKQPIWGSLSRSLTLSSQRVPEFS
SFVARTNTCGELRSSHLGQEVTLCGWIQYRRQNTFLVLRDCHGLVQILIPQDESAASV
RRTLCEAPVESVVRVSGTVIARPLGQENPKMPTGEIEIKAKTAELLNACKKLPFEIKD
FVKKTEALRLQYRYLDLRSSQMQHNLRLRSQMVMKMREYLCTLHGFVDIETPTLFKRT
PGGAKEFLVPSREPGRFYSLPQSPQQFKQLLMVGGLDRYFQVARCYRDEGSRPDRQPE
FTQIDIEMSFVDQTGIQHLVEGLLHYSWPEDKDPLVAPFPSMTFAEALATYGTDKPDT
RFGMKIVDISDVFRNTEIRFLQDALAKPQGTVKAICVHEGAKYLRKEDIEFIRKFAAH
HFSQEVLPIFLNARKNWSSPFAKFITEEERLELTRLMEIQEDDMVLLTAGQHEKACSL
LGKLRLECADLLETRGLALRDPALFSFLWVLDFPLFLAKEESPTELESAHHPFTAPHP
GDIHLLYTEPEKVRGQHYDLVLNGNEIGGGSIRIHDAQLQRYILETLLKEDVKLLSHL
LQALDYGAPPHGGIALGLDRLVCLVTGAPSIRDVIAFPKSYRGHDLMSNAPDTVSPED
LKPYHIHVSWPTDSEERASATPSKYLSS"
transit_peptide 511..648
/gene="Dars2"
/gene_synonym="RGD1308286"
/note="Mitochondrion. /evidence=ECO:0000255; propagated
from UniProtKB/Swiss-Prot (Q3KRD0.1)"
mat_peptide 649..2466
/gene="Dars2"
/gene_synonym="RGD1308286"
/product="Aspartate--tRNA ligase, mitochondrial.
/id=PRO_0000250738"
/note="propagated from UniProtKB/Swiss-Prot (Q3KRD0.1)"
misc_feature 1162..1164
/gene="Dars2"
/gene_synonym="RGD1308286"
/note="Phosphothreonine.
/evidence=ECO:0007744|PubMed:16641100; propagated from
UniProtKB/Swiss-Prot (Q3KRD0.1); phosphorylation site"
misc_feature 1231..1233
/gene="Dars2"
/gene_synonym="RGD1308286"
/note="Phosphoserine.
/evidence=ECO:0000250|UniProtKB:Q6PI48; propagated from
UniProtKB/Swiss-Prot (Q3KRD0.1); phosphorylation site"
misc_feature 1237..1248
/gene="Dars2"
/gene_synonym="RGD1308286"
/note="propagated from UniProtKB/Swiss-Prot (Q3KRD0.1);
Region: Aspartate. /evidence=ECO:0000250"
exon 635..734
/gene="Dars2"
/gene_synonym="RGD1308286"
/inference="alignment:Splign:2.1.0"
exon 735..801
/gene="Dars2"
/gene_synonym="RGD1308286"
/inference="alignment:Splign:2.1.0"
exon 802..903
/gene="Dars2"
/gene_synonym="RGD1308286"
/inference="alignment:Splign:2.1.0"
exon 904..999
/gene="Dars2"
/gene_synonym="RGD1308286"
/inference="alignment:Splign:2.1.0"
exon 1000..1123
/gene="Dars2"
/gene_synonym="RGD1308286"
/inference="alignment:Splign:2.1.0"
exon 1124..1170
/gene="Dars2"
/gene_synonym="RGD1308286"
/inference="alignment:Splign:2.1.0"
exon 1171..1277
/gene="Dars2"
/gene_synonym="RGD1308286"
/inference="alignment:Splign:2.1.0"
exon 1278..1347
/gene="Dars2"
/gene_synonym="RGD1308286"
/inference="alignment:Splign:2.1.0"
exon 1348..1527
/gene="Dars2"
/gene_synonym="RGD1308286"
/inference="alignment:Splign:2.1.0"
exon 1528..1635
/gene="Dars2"
/gene_synonym="RGD1308286"
/inference="alignment:Splign:2.1.0"
exon 1636..1698
/gene="Dars2"
/gene_synonym="RGD1308286"
/inference="alignment:Splign:2.1.0"
exon 1699..1851
/gene="Dars2"
/gene_synonym="RGD1308286"
/inference="alignment:Splign:2.1.0"
exon 1852..2070
/gene="Dars2"
/gene_synonym="RGD1308286"
/inference="alignment:Splign:2.1.0"
exon 2071..2181
/gene="Dars2"
/gene_synonym="RGD1308286"
/inference="alignment:Splign:2.1.0"
exon 2182..2257
/gene="Dars2"
/gene_synonym="RGD1308286"
/inference="alignment:Splign:2.1.0"
exon 2258..2686
/gene="Dars2"
/gene_synonym="RGD1308286"
/inference="alignment:Splign:2.1.0"
ORIGIN
1 agagggacgg gcttctggat gtgatttgtg cctgggttcg aacctcagag ttctttggat
61 ttgctcgctt tcacctttca gaaaaattca agatgggccg ccaggtttgg gagagttgaa
121 acacgaggct gtacctcctt atgattctgc ttatgccccg gtcaccttcg aggaacgtgg
181 gaataaaggg aggacgtgcg cctggtgact ggccttccgg ctggggtcgc tctgatgctg
241 gcgctgctgt ttgggatctt ttggtatctt cggggcgcag ccttgtttaa ttaaagtggg
301 aactgatgca ctgaaggaag gcttgcccta cccactcatc cacgggatct agagaccttg
361 cagatcaatc tatttgtaga tacttgaacg ttgtgcggag gagggcctgg atgcatttgc
421 gtgggaagta gaattttaag ggttccctgg tattccaaag ccgagttttg agcaccacca
481 cacctgcagc gcggattccg ggacttcaac atgtatcttg gttcttggtt aaatcgtcta
541 ggcaggggtt tatccagacc catcggaaag accaagcagc caatctgggg ttctctttcc
601 agaagtctga cgttgagttc acagagagtt ccagaattca gtagctttgt tgctcggacc
661 aacacatgtg gagaacttcg ttcttcccac ttaggccaag aagtcactct gtgtggatgg
721 attcagtacc gaaggcaaaa caccttctta gtcctaagag attgccacgg tctcgttcaa
781 atcctcattc cccaggatga gtcggctgca tctgtgagga ggactttgtg tgaagcccct
841 gtggagtctg tggtgagagt atctgggacg gtcatcgccc gacccctggg acaggagaat
901 ccgaaaatgc caacaggtga gattgaaatc aaagctaaaa cagctgagct cctgaatgcc
961 tgcaaaaagc tgccctttga aattaaagac tttgtgaaga agacggaagc ccttcgtctg
1021 cagtatcggt acttagacct gcgcagctcc caaatgcagc acaacctgcg gctcaggtca
1081 cagatggtca tgaaaatgcg ggaatatctc tgtactctgc acggatttgt ggatatagaa
1141 accccaacat tgtttaagag gactcctgga ggtgccaagg agtttttagt accgtccagg
1201 gaacctggaa ggttttattc tctccctcag agtcctcaac agtttaagca gcttctgatg
1261 gttggtggtt tagacaggta ctttcaggtt gcccggtgtt atcgagatga aggctcaaga
1321 ccggataggc agccagagtt tactcagatt gacatagaga tgtcctttgt agaccagact
1381 ggcattcagc atttagttga gggtttgctg cattattcct ggcctgaaga taaagaccct
1441 ctagtggctc cttttccttc catgactttt gctgaggcat tggctaccta cggaactgat
1501 aagccagata ctcgctttgg gatgaagatt gtggatatca gtgatgtgtt cagaaacaca
1561 gagatcaggt ttcttcagga tgcactggct aagccccaag gaactgtcaa agcaatatgt
1621 gttcatgaag gagcaaagta cttaaggaaa gaagacattg agttcattcg aaagtttgca
1681 gctcaccatt tcagtcagga agtccttcct atattcctga atgccaggaa gaactggagt
1741 tcaccatttg ctaaattcat aacggaggag gaaagattgg agttaaccag attgatggag
1801 atacaagaag acgacatggt gctgctgact gctgggcaac atgagaaagc atgctctttg
1861 ttggggaagt tacgactaga gtgtgcagac cttttggaaa cgagaggact ggcgctcagg
1921 gacccagcat tgttctcttt tctctgggta cttgacttcc cactcttcct cgccaaggaa
1981 gagagtccca cagagctgga gtcagcccac cacccattta ctgctcctca ccccggcgac
2041 attcacctcc tctacactga gccggaaaag gttcgtggcc aacactacga cttggtttta
2101 aatggcaatg agataggagg aggctctatc cgcattcatg atgcacagct acagaggtac
2161 atcctggaaa cattgctaaa ggaagatgtg aaattgcttt cccatctgct tcaggcttta
2221 gactatgggg cacccccaca tggaggaatt gccttagggt tagacagact ggtgtgtctt
2281 gtcacgggag cacccagcat cagagatgtc atagccttcc ctaaatccta ccgaggacat
2341 gaccttatga gcaatgctcc agacactgtg tctcctgaag atctgaagcc ttaccatatc
2401 cacgtctcat ggccaacaga ctcagaagag agagcatccg ccacaccttc caaatacttg
2461 agctcttgac ttgtcttctt tgctttacta aacctaaaaa gaagatccag acttgccaca
2521 gactttagaa tcaagtttct gtgtagaagc aatcattacc gagtaagaaa cagaagctac
2581 acaattgtgg tcatatgtac cattaggact ttctgtagtt gggagttctg tggaagtttt
2641 ttaattggag aattgtaaag tgaaacaaaa aaagcttttt attgga
//