The inhibitory effect of microRNA-1827 on anoikis resistance in lung adenocarcinoma A549 cells via targeting caveolin-1

Acta Biochim Biophys Sin (Shanghai). 2020 Oct 19;52(10):1148-1155. doi: 10.1093/abbs/gmaa102.

Abstract

Anoikis resistance is a critical process for cancer cell metastasis in non-small cell lung cancer (NSCLC), and microRNA-1827 (miR-1827) is closely correlated with NSCLC metastasis. In this study, we aimed to evaluate the role of miR-1827 in regulating the anoikis resistance of NSCLC. The results showed that miR-1827 level was decreased in tumor tissues and cells and was correlated with tumor grade and lymph node (LN) metastasis. Overexpression of miR-1827 inhibited anchorage-independent growth and anoikis resistance in A549 cells. Bioinformatics and functional analysis identified that caveolin-1 (CAV-1) is directly targeted by miR-1827. Restoration of CAV-1 significantly attenuated miR-1827's effect on anoikis resistance in A549 cells. Our data identified a novel signaling axis of miR-1827/CAV-1 in regulating anoikis resistance, which might serve as a potential therapeutic target for metastatic NSCLC.

Keywords: A549 cell; NSCLC; anoikis resistance; caveolin-1; microRNA-1827.

MeSH terms

  • A549 Cells
  • Adenocarcinoma of Lung / genetics*
  • Adult
  • Aged
  • Anoikis / genetics*
  • Carcinoma, Non-Small-Cell Lung / genetics*
  • Caveolin 1 / metabolism*
  • Cell Movement / genetics
  • Cell Survival / genetics
  • Computational Biology
  • Down-Regulation
  • Female
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Lung Neoplasms / genetics*
  • Lymphatic Metastasis / genetics
  • Male
  • MicroRNAs / metabolism*
  • Middle Aged
  • Neoplasm Staging
  • Signal Transduction

Substances

  • CAV1 protein, human
  • Caveolin 1
  • MIRN1827 microRNA, human
  • MicroRNAs