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Conserved domains on  [gi|1799708781|pdb|6PAF|A]
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Chain A, Histone-lysine N-methyltransferase SMYD3

Protein Classification

histone-lysine N-methyltransferase SMYD3( domain architecture ID 14443467)

histone-lysine N-methyltransferase SMYD3 specifically methylates 'Lys-4' of histone H3, inducing di- and tri-methylation, but not monomethylation; also methylates 'Lys-5' of histone H4

CATH:  1.25.40.10|1.25.40.970|3.30.70.3410|3.30.60.180|2.170.270.10
EC:  2.1.1.354
Gene Ontology:  GO:0032259|GO:0042054|GO:0046872|GO:0000976|GO:0000993|GO:1904047
PubMed:  16086857|25580534|25125178|34555372|24710145
SCOP:  4000530

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
SET_SMYD3 cd19203
SET domain (including post-SET domain) found in SET and MYND domain-containing protein 3 ...
 9-272 1.01e-121

SET domain (including post-SET domain) found in SET and MYND domain-containing protein 3 (SMYD3) and similar proteins; SMYD3 (also termed zinc finger MYND domain-containing protein 1) functions as a histone methyltransferase that specifically methylates 'Lys-4' of histone H3, inducing di- and tri-methylation, but not monomethylation. It also methylates 'Lys-5' of histone H4. SMYD3 plays an important role in transcriptional activation as a member of an RNA polymerase complex. It is overexpressed in colorectal, breast, prostate, and hepatocellular tumors, and has been implicated as an oncogene in human malignancies. Methylation of MEKK2 by SMYD3 is important for regulation of the MEK/ERK pathway, suggesting the possibility of selectively targeting SMYD3 in RAS-driven cancers.


:

Pssm-ID: 380980 [Multi-domain]  Cd Length: 210  Bit Score: 352.44  E-value: 1.01e-121
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
6PAF_A        9 KVEKFATANRGNGLRAVTPLRPGELLFRSDPLAYTVCKgsrgvvcdrcllgkeklmrcsqcrvakycsakcqkkawpdhk 88
Cdd:cd19203   2 KVEKFETAEKGRGLRAVTPLKPGELVLKADPFAYTVCN------------------------------------------ 39

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
6PAF_A       89 reckclksckpryPPDSVRLLGRVVFKLMDGAPSESEKLYSFYDLESNINKLTEDKKEGLRQLVMTFQHFMREEIQDASQ 168
Cdd:cd19203  40 -------------PPDSVRLEGRIIFKLLDKAPSESEKLYSFYDLQSNINKLSEDRKEGLRQLAMVLQHYLREEIQDASQ 106

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
6PAF_A      169 LPPAFDLFEAFAKVICNSFTICNAEMQEVGVGLYPSISLLNHSCDPNCSIVFNGPHLLLRAVRDIEVGEELTICYLDMLM 248
Cdd:cd19203 107 LPPAFDIFELFAKVTCNSFTICDAEMQEVGVGLYPSASLLNHSCDPNCVIVFNGPHLLLRAIREIEVGEELTISYIDMLM 186

                       250       260
                ....*....|....*....|....
6PAF_A      249 TSEERRKQLRDQYCFECDCFRCQT 272
Cdd:cd19203 187 PSEERRKQLRDQYCFECDCFRCQD 210
zf-MYND pfam01753
MYND finger;
53-91 1.05e-13

MYND finger;


:

Pssm-ID: 460312  Cd Length: 39  Bit Score: 64.75  E-value: 1.05e-13
                          10        20        30
                  ....*....|....*....|....*....|....*....
6PAF_A         53 CDRCLLGKEKLMRCSQCRVAKYCSAKCQKKAWPDHKREC 91
Cdd:pfam01753   1 CAVCGKEALKLLRCSRCKSVYYCSKECQKADWPYHKKEC 39
 
Name Accession Description Interval E-value
SET_SMYD3 cd19203
SET domain (including post-SET domain) found in SET and MYND domain-containing protein 3 ...
 9-272 1.01e-121

SET domain (including post-SET domain) found in SET and MYND domain-containing protein 3 (SMYD3) and similar proteins; SMYD3 (also termed zinc finger MYND domain-containing protein 1) functions as a histone methyltransferase that specifically methylates 'Lys-4' of histone H3, inducing di- and tri-methylation, but not monomethylation. It also methylates 'Lys-5' of histone H4. SMYD3 plays an important role in transcriptional activation as a member of an RNA polymerase complex. It is overexpressed in colorectal, breast, prostate, and hepatocellular tumors, and has been implicated as an oncogene in human malignancies. Methylation of MEKK2 by SMYD3 is important for regulation of the MEK/ERK pathway, suggesting the possibility of selectively targeting SMYD3 in RAS-driven cancers.


Pssm-ID: 380980 [Multi-domain]  Cd Length: 210  Bit Score: 352.44  E-value: 1.01e-121
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
6PAF_A        9 KVEKFATANRGNGLRAVTPLRPGELLFRSDPLAYTVCKgsrgvvcdrcllgkeklmrcsqcrvakycsakcqkkawpdhk 88
Cdd:cd19203   2 KVEKFETAEKGRGLRAVTPLKPGELVLKADPFAYTVCN------------------------------------------ 39

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
6PAF_A       89 reckclksckpryPPDSVRLLGRVVFKLMDGAPSESEKLYSFYDLESNINKLTEDKKEGLRQLVMTFQHFMREEIQDASQ 168
Cdd:cd19203  40 -------------PPDSVRLEGRIIFKLLDKAPSESEKLYSFYDLQSNINKLSEDRKEGLRQLAMVLQHYLREEIQDASQ 106

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
6PAF_A      169 LPPAFDLFEAFAKVICNSFTICNAEMQEVGVGLYPSISLLNHSCDPNCSIVFNGPHLLLRAVRDIEVGEELTICYLDMLM 248
Cdd:cd19203 107 LPPAFDIFELFAKVTCNSFTICDAEMQEVGVGLYPSASLLNHSCDPNCVIVFNGPHLLLRAIREIEVGEELTISYIDMLM 186

                       250       260
                ....*....|....*....|....
6PAF_A      249 TSEERRKQLRDQYCFECDCFRCQT 272
Cdd:cd19203 187 PSEERRKQLRDQYCFECDCFRCQD 210
zf-MYND pfam01753
MYND finger;
53-91 1.05e-13

MYND finger;


Pssm-ID: 460312  Cd Length: 39  Bit Score: 64.75  E-value: 1.05e-13
                          10        20        30
                  ....*....|....*....|....*....|....*....
6PAF_A         53 CDRCLLGKEKLMRCSQCRVAKYCSAKCQKKAWPDHKREC 91
Cdd:pfam01753   1 CAVCGKEALKLLRCSRCKSVYYCSKECQKADWPYHKKEC 39
SET pfam00856
SET domain; SET domains are protein lysine methyltransferase enzymes. SET domains appear to be ...
 207-243 3.70e-12

SET domain; SET domains are protein lysine methyltransferase enzymes. SET domains appear to be protein-protein interaction domains. It has been demonstrated that SET domains mediate interactions with a family of proteins that display similarity with dual-specificity phosphatases (dsPTPases). A subset of SET domains have been called PR domains. These domains are divergent in sequence from other SET domains, but also appear to mediate protein-protein interaction. The SET domain consists of two regions known as SET-N and SET-C. SET-C forms an unusual and conserved knot-like structure of probably functional importance. Additionally to SET-N and SET-C, an insert region (SET-I) and flanking regions of high structural variability form part of the overall structure.


Pssm-ID: 459965 [Multi-domain]  Cd Length: 115  Bit Score: 62.93  E-value: 3.70e-12
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|.
6PAF_A        207 LLNHSCDPNCSIVF----NGPHLLLRAVRDIEVGEELTICY 243
Cdd:pfam00856  74 FINHSCDPNCEVRVvyvnGGPRIVIFALRDIKPGEELTIDY 114
SET smart00317
SET (Su(var)3-9, Enhancer-of-zeste, Trithorax) domain; Putative methyl transferase, based on ...
 152-243 4.36e-11

SET (Su(var)3-9, Enhancer-of-zeste, Trithorax) domain; Putative methyl transferase, based on outlier plant homologues


Pssm-ID: 214614 [Multi-domain]  Cd Length: 124  Bit Score: 60.04  E-value: 4.36e-11
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
6PAF_A         152 VMTFQHFMREEIQDASQLPPAFDLFEAFAKVICNsfticnaemqevGVGLYPSISLLNHSCDPNCSIVF----NGPHLLL 227
Cdd:smart00317  34 IITSEEAEERPKAYDTDGAKAFYLFDIDSDLCID------------ARRKGNLARFINHSCEPNCELLFvevnGDDRIVI 101

                           90
                   ....*....|....*.
6PAF_A         228 RAVRDIEVGEELTICY 243
Cdd:smart00317 102 FALRDIKPGEELTIDY 117
SET COG2940
SET domain-containing protein (function unknown) [General function prediction only];
206-243 1.15e-10

SET domain-containing protein (function unknown) [General function prediction only];


Pssm-ID: 442183 [Multi-domain]  Cd Length: 134  Bit Score: 59.20  E-value: 1.15e-10
                        10        20        30
                ....*....|....*....|....*....|....*...
6PAF_A      206 SLLNHSCDPNCSIVFNGPHLLLRAVRDIEVGEELTICY 243
Cdd:COG2940  78 RFINHSCDPNCEADEEDGRIFIVALRDIAAGEELTYDY 115
 
Name Accession Description Interval E-value
SET_SMYD3 cd19203
SET domain (including post-SET domain) found in SET and MYND domain-containing protein 3 ...
 9-272 1.01e-121

SET domain (including post-SET domain) found in SET and MYND domain-containing protein 3 (SMYD3) and similar proteins; SMYD3 (also termed zinc finger MYND domain-containing protein 1) functions as a histone methyltransferase that specifically methylates 'Lys-4' of histone H3, inducing di- and tri-methylation, but not monomethylation. It also methylates 'Lys-5' of histone H4. SMYD3 plays an important role in transcriptional activation as a member of an RNA polymerase complex. It is overexpressed in colorectal, breast, prostate, and hepatocellular tumors, and has been implicated as an oncogene in human malignancies. Methylation of MEKK2 by SMYD3 is important for regulation of the MEK/ERK pathway, suggesting the possibility of selectively targeting SMYD3 in RAS-driven cancers.


Pssm-ID: 380980 [Multi-domain]  Cd Length: 210  Bit Score: 352.44  E-value: 1.01e-121
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
6PAF_A        9 KVEKFATANRGNGLRAVTPLRPGELLFRSDPLAYTVCKgsrgvvcdrcllgkeklmrcsqcrvakycsakcqkkawpdhk 88
Cdd:cd19203   2 KVEKFETAEKGRGLRAVTPLKPGELVLKADPFAYTVCN------------------------------------------ 39

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
6PAF_A       89 reckclksckpryPPDSVRLLGRVVFKLMDGAPSESEKLYSFYDLESNINKLTEDKKEGLRQLVMTFQHFMREEIQDASQ 168
Cdd:cd19203  40 -------------PPDSVRLEGRIIFKLLDKAPSESEKLYSFYDLQSNINKLSEDRKEGLRQLAMVLQHYLREEIQDASQ 106

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
6PAF_A      169 LPPAFDLFEAFAKVICNSFTICNAEMQEVGVGLYPSISLLNHSCDPNCSIVFNGPHLLLRAVRDIEVGEELTICYLDMLM 248
Cdd:cd19203 107 LPPAFDIFELFAKVTCNSFTICDAEMQEVGVGLYPSASLLNHSCDPNCVIVFNGPHLLLRAIREIEVGEELTISYIDMLM 186

                       250       260
                ....*....|....*....|....
6PAF_A      249 TSEERRKQLRDQYCFECDCFRCQT 272
Cdd:cd19203 187 PSEERRKQLRDQYCFECDCFRCQD 210
SET_SMYD1_2_3-like cd19167
SET domain (including post-SET domain) found in SET and MYND domain-containing proteins, SMYD1, ...
 8-272 5.80e-107

SET domain (including post-SET domain) found in SET and MYND domain-containing proteins, SMYD1, SMYD2, SMYD3 and similar proteins; The family includes SET and MYND domain-containing proteins, SMYD1, SMYD2 and SMYD3. SMYD1 (EC 2.1.1.43; also termed BOP) is a heart and muscle specific SET-MYND domain containing protein, which functions as a histone methyltransferase and regulates downstream gene transcription. It methylates histone H3 at 'Lys-4' (H3K4me), seems able to perform both mono-, di-, and trimethylation. SMYD2 (also termed HSKM-B, or lysine N-methyltransferase 3C (KMT3C)) functions as a histone methyltransferase that methylates both histones and non-histone proteins, including p53/TP53 and RB1. It specifically methylates histone H3 'Lys-4' (H3K4me) and dimethylates histone H3 'Lys-36' (H3K36me2). SMYD3 (also termed zinc finger MYND domain-containing protein 1) functions as a histone methyltransferase that specifically methylates 'Lys-4' of histone H3, inducing di- and tri-methylation, but not monomethylation. It also methylates 'Lys-5' of histone H4. SMYD3 plays an important role in transcriptional activation as a member of an RNA polymerase complex.


Pssm-ID: 380944 [Multi-domain]  Cd Length: 205  Bit Score: 314.75  E-value: 5.80e-107
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
6PAF_A        8 LKVEKFATANRGNGLRAVTPLRPGELLFRSDPLAYTVCkgsrgvvcdrcllgkeklmrcsqcrvakycsakcqkkawpdh 87
Cdd:cd19167   1 GQVERFCSPGKGRGLRALKPFEPGDLIFSERPYAYVLT------------------------------------------ 38

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
6PAF_A       88 kreckclksckpryPPDSVRLLGRVVFKLMDGAPSESEKLYSFYDLESNINKLTEDKKEGLRQLVMTFQHFMREEIQdas 167
Cdd:cd19167  39 --------------PPEHVRLTGRILYKQHIRKTRTSGKLLSVYDLESHVEKLDEEKKDGLRSDVATLHQFMSKDLQ--- 101

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
6PAF_A      168 qLPPAFDLFEAFAKVICNSFTICNAEMQEVGVGLYPSISLLNHSCDPNCSIVFNGPHLLLRAVRDIEVGEELTICYLDML 247
Cdd:cd19167 102 -LPDAAYLVELFGKVNCNGFTISDEELQHVGVGIYPQAALLNHSCCPNCIVTFNGPNIEVRAVQEIEPGEEVFHSYIDLL 180

                       250       260
                ....*....|....*....|....*
6PAF_A      248 MTSEERRKQLRDQYCFECDCFRCQT 272
Cdd:cd19167 181 YPTEERRDQLRDQYFFLCQCADCQT 205
SET_SMYD1 cd10526
SET domain (including post-SET domain) found in SET and MYND domain-containing protein 1 ...
 8-271 8.05e-43

SET domain (including post-SET domain) found in SET and MYND domain-containing protein 1 (SMYD1) and similar proteins; SMYD1 (EC 2.1.1.43), also termed BOP, is a heart and muscle specific SET-MYND domain containing protein, which functions as a histone methyltransferase and regulates downstream gene transcription. It methylates histone H3 at 'Lys-4' (H3K4me), seems able to perform both mono-, di-, and trimethylation. SMYD1 plays a critical role in cardiomyocyte differentiation, cardiac morphogenesis and myofibril organization, as well as in the regulation of endothelial cells (ECs). It is expressed in vascular endothelial cells, it has beenshown that knockdown of SMYD1 in endothelial cells impairs EC migration and tube formation.


Pssm-ID: 380924 [Multi-domain]  Cd Length: 210  Bit Score: 149.49  E-value: 8.05e-43
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
6PAF_A        8 LKVEKFATANRGNGLRAVTPLRPGELLFRSDPLAYTVCkgsrgvvcdrcllgkeklmrcsqcrvakycsakcqkkawpdh 87
Cdd:cd10526   1 EGVEVFTSEGKGRGLKATKEFWAGDVIFAEPPYAAVVF------------------------------------------ 38

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
6PAF_A       88 kreckclksckPRYPPDSVRLLGRVVFKL-MDGAPSESEKLYSFYDLESNINKLTEDKKEGLRQLVMTF-QHFMREEIQD 165
Cdd:cd10526  39 -----------GKAPNENIRLAARILWRIeREGGGLKEGELVSIEDLQDHLEDFSEEEKKDLREDVHSFlDYWPYQSQQF 107

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
6PAF_A      166 ASQlppafDLFEAFAKVICNSFTICNAE-MQEVGVGLYPSISLLNHSCDPNCSIVFNGPHLLLRAVRDIEVGEELTICYL 244
Cdd:cd10526 108 SME-----YISHIFGVINCNGFTLSDQRgLQAVGVGIFPNLCLVNHDCWPNCTVIFNNGRIELRALGKISEGDELTVSYI 182

                       250       260
                ....*....|....*....|....*..
6PAF_A      245 DMLMTSEERRKQLRDQYCFECDCFRCQ 271
Cdd:cd10526 183 DFLNTSEDRKEQLKKQYYFDCTCEHCT 209
SET_SMYD cd20071
SET domain (including SET domain and post-SET domain) found in SET and MYND domain-containing ...
 176-270 5.05e-35

SET domain (including SET domain and post-SET domain) found in SET and MYND domain-containing protein, and similar proteins; The family includes SET and MYND domain-containing proteins, SMYD1-SYMD5. SMYD1 (EC 2.1.1.43; also termed BOP) is a heart and muscle specific SET-MYND domain containing protein, which functions as a histone methyltransferase and regulates downstream gene transcription. It methylates histone H3 at 'Lys-4' (H3K4me), seems able to perform both mono-, di-, and trimethylation. SMYD2 (also termed HSKM-B, or lysine N-methyltransferase 3C (KMT3C)) functions as a histone methyltransferase that methylates both histones and non-histone proteins, including p53/TP53 and RB1. It specifically methylates histone H3 'Lys-4' (H3K4me) and dimethylates histone H3 'Lys-36' (H3K36me2). SMYD3 (also termed zinc finger MYND domain-containing protein 1) functions as a histone methyltransferase that specifically methylates 'Lys-4' of histone H3, inducing di- and tri-methylation, but not monomethylation. It also methylates 'Lys-5' of histone H4. SMYD3 plays an important role in transcriptional activation as a member of an RNA polymerase complex. SMYD4 functions as a potential tumor suppressor that plays a critical role in breast carcinogenesis at least partly through inhibiting the expression of PDGFR-alpha. SMYD5 (also termed protein NN8-4AG, or retinoic acid-induced protein 15) functions as histone lysine methyltransferase that mediates H4K20me3 at heterochromatin regions.


Pssm-ID: 380997 [Multi-domain]  Cd Length: 122  Bit Score: 125.95  E-value: 5.05e-35
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
6PAF_A      176 FEAFAKVICNSFTICNaEMQEVGVGLYPSISLLNHSCDPNCSIVFNGPHLL-LRAVRDIEVGEELTICYLDMLMTSEERR 254
Cdd:cd20071  28 EKPLVSVPSNSFSLTD-GLNEIGVGLFPLASLLNHSCDPNAVVVFDGNGTLrVRALRDIKAGEELTISYIDPLLPRTERR 106

                        90
                ....*....|....*.
6PAF_A      255 KQLRDQYCFECDCFRC 270
Cdd:cd20071 107 RELLEKYGFTCSCPRC 122
SET_SMYD2 cd19202
SET domain (including post-SET domain) found in SET and MYND domain-containing protein 2 ...
 10-272 2.23e-32

SET domain (including post-SET domain) found in SET and MYND domain-containing protein 2 (SMYD2) and similar proteins; SMYD2 (also termed HSKM-B, lysine N-methyltransferase 3C (KMT3C)) functions as a histone methyltransferase that methylates both histones and non-histone proteins, including p53/TP53 and RB1. It specifically methylates histone H3 'Lys-4' (H3K4me) and dimethylates histone H3 'Lys-36' (H3K36me2). It plays a role in myofilament organization in both skeletal and cardiac muscles via Hsp90 methylation. SMYD2 overexpression is associated with tumor cell proliferation and a worse outcome in human papillomavirus-unrelated nonmultiple head and neck carcinomas. It regulates leukemia cell growth such that diminished SMYD2 expression upregulates SET7/9, thereby possibly shifting leukemia cells from growth to quiescence state associated with resistance to DNA damage associated with Acute Myeloid Leukemia (AML).


Pssm-ID: 380979 [Multi-domain]  Cd Length: 206  Bit Score: 121.85  E-value: 2.23e-32
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
6PAF_A       10 VEKFATANRGNGLRAVTPLRPGELLFrSDPlAYTvckgsrgvvcdrCLLGkeklmrcsqcrvakycsakcqkkawpdhkr 89
Cdd:cd19202   3 LERFCSPGKGRGLRALRPFQVGDLLF-SCP-AYA------------YVLT------------------------------ 38

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
6PAF_A       90 eckclksckpryPPDSVRLLGRVVFKL-MDGAPSESEKLYSFYDLESNINKLTEDKKEGLRQLVMTFQHFMREEIQdasq 168
Cdd:cd19202  39 ------------PSETVRLTARILAKQkIHPERTPSEKLLAVKEFESHLDKLDNEKKDLIQSDIAALHHFYSKHLE---- 102

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
6PAF_A      169 LPPAFDLFEAFAKVICNSFTICNAEMQEVGVGLYPSISLLNHSCDPNCSIVFNGPHLLLRAVRDIEVGEELTICYLDMLM 248
Cdd:cd19202 103 FPDNDSLVVLFAQVNCNGFTIEDEELSHLGSAIFPDVALMNHSCCPNVIVTYKGTLAEVRAVQEIKPGEEVFTSYIDLLY 182

                       250       260
                ....*....|....*....|....
6PAF_A      249 TSEERRKQLRDQYCFECDCFRCQT 272
Cdd:cd19202 183 PTEDRNDRLRDSYFFTCECQECTT 206
SET_SMYD4 cd10536
SET domain (including iSET domain and post-SET domain) found in SET and MYND domain-containing ...
 184-270 2.15e-25

SET domain (including iSET domain and post-SET domain) found in SET and MYND domain-containing protein 4 (SMYD4) and similar proteins; SMYD4 functions as a potential tumor suppressor that plays a critical role in breast carcinogenesis at least partly through inhibiting the expression of PDGFR-alpha. In zebrafish, SMYD4 is ubiquitously expressed in early embryos and becomes enriched in the developing heart; mutants show a strong defect in cardiomyocyte proliferation, which lead to a severe cardiac malformation.


Pssm-ID: 380934 [Multi-domain]  Cd Length: 218  Bit Score: 103.15  E-value: 2.15e-25
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
6PAF_A      184 CNSFTI------------CNAEMQEVGVGLYPSISLLNHSCDPNCSIVFNGPHLLLRAVRDIEVGEELTICYLDM--LMT 249
Cdd:cd10536 118 CNAHAItelqttssgsqvDTSKQVRIATAIYPTLSLLNHSCDPNTIRSFYGNTIVVRATRPIKKGEEITICYGPHfsRMK 197

                        90       100
                ....*....|....*....|.
6PAF_A      250 SEERRKQLRDQYCFECDCFRC 270
Cdd:cd10536 198 RSERQRLLKEQYFFDCSCEAC 218
SET_SMYD5 cd10521
SET domain (including iSET domain and post-SET domain) found in SET and MYND domain-containing ...
 198-273 3.10e-17

SET domain (including iSET domain and post-SET domain) found in SET and MYND domain-containing protein 5 (SMYD5) and similar proteins; SMYD5 (also termed protein NN8-4AG, or retinoic acid-induced protein 15) functions as histone lysine methyltransferase that mediates H4K20me3 at heterochromatin regions. It plays an important role in chromosome integrity by regulating heterochromatin and repressing endogenous repetitive DNA elements during differentiation. In zebrafish embryogenesis, it plays pivotal roles in both primitive and definitive hematopoiesis.


Pssm-ID: 380919 [Multi-domain]  Cd Length: 282  Bit Score: 81.59  E-value: 3.10e-17
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
6PAF_A      198 GVGLYPSISLLNHSCDPNCSIVF--NGPHLLLRAVRDIEVGEELTICYLDMLMTSEE---RRKQLRDQYCFECDCFRCQT 272
Cdd:cd10521 202 GSGLYLLQSCCNHSCVPNAEITFpeNNFTLSLKALRDIQEGEEICISYLDECQRERSrhsRQKILRENYLFICNCPKCEA 281


                .
6PAF_A      273 Q 273
Cdd:cd10521 282 Q 282
zf-MYND pfam01753
MYND finger;
53-91 1.05e-13

MYND finger;


Pssm-ID: 460312  Cd Length: 39  Bit Score: 64.75  E-value: 1.05e-13
                          10        20        30
                  ....*....|....*....|....*....|....*....
6PAF_A         53 CDRCLLGKEKLMRCSQCRVAKYCSAKCQKKAWPDHKREC 91
Cdd:pfam01753   1 CAVCGKEALKLLRCSRCKSVYYCSKECQKADWPYHKKEC 39
SET pfam00856
SET domain; SET domains are protein lysine methyltransferase enzymes. SET domains appear to be ...
 207-243 3.70e-12

SET domain; SET domains are protein lysine methyltransferase enzymes. SET domains appear to be protein-protein interaction domains. It has been demonstrated that SET domains mediate interactions with a family of proteins that display similarity with dual-specificity phosphatases (dsPTPases). A subset of SET domains have been called PR domains. These domains are divergent in sequence from other SET domains, but also appear to mediate protein-protein interaction. The SET domain consists of two regions known as SET-N and SET-C. SET-C forms an unusual and conserved knot-like structure of probably functional importance. Additionally to SET-N and SET-C, an insert region (SET-I) and flanking regions of high structural variability form part of the overall structure.


Pssm-ID: 459965 [Multi-domain]  Cd Length: 115  Bit Score: 62.93  E-value: 3.70e-12
                          10        20        30        40
                  ....*....|....*....|....*....|....*....|.
6PAF_A        207 LLNHSCDPNCSIVF----NGPHLLLRAVRDIEVGEELTICY 243
Cdd:pfam00856  74 FINHSCDPNCEVRVvyvnGGPRIVIFALRDIKPGEELTIDY 114
SET smart00317
SET (Su(var)3-9, Enhancer-of-zeste, Trithorax) domain; Putative methyl transferase, based on ...
 152-243 4.36e-11

SET (Su(var)3-9, Enhancer-of-zeste, Trithorax) domain; Putative methyl transferase, based on outlier plant homologues


Pssm-ID: 214614 [Multi-domain]  Cd Length: 124  Bit Score: 60.04  E-value: 4.36e-11
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
6PAF_A         152 VMTFQHFMREEIQDASQLPPAFDLFEAFAKVICNsfticnaemqevGVGLYPSISLLNHSCDPNCSIVF----NGPHLLL 227
Cdd:smart00317  34 IITSEEAEERPKAYDTDGAKAFYLFDIDSDLCID------------ARRKGNLARFINHSCEPNCELLFvevnGDDRIVI 101

                           90
                   ....*....|....*.
6PAF_A         228 RAVRDIEVGEELTICY 243
Cdd:smart00317 102 FALRDIKPGEELTIDY 117
SET COG2940
SET domain-containing protein (function unknown) [General function prediction only];
206-243 1.15e-10

SET domain-containing protein (function unknown) [General function prediction only];


Pssm-ID: 442183 [Multi-domain]  Cd Length: 134  Bit Score: 59.20  E-value: 1.15e-10
                        10        20        30
                ....*....|....*....|....*....|....*...
6PAF_A      206 SLLNHSCDPNCSIVFNGPHLLLRAVRDIEVGEELTICY 243
Cdd:COG2940  78 RFINHSCDPNCEADEEDGRIFIVALRDIAAGEELTYDY 115
SET cd08161
SET (Su(var)3-9, Enhancer-of-zeste, Trithorax) domain superfamily; The Su(var)3-9, ...
 209-243 5.53e-10

SET (Su(var)3-9, Enhancer-of-zeste, Trithorax) domain superfamily; The Su(var)3-9, Enhancer-of-zeste, Trithorax (SET) domain superfamily corresponds to SET domain-containing lysine methyltransferases, which catalyze site and state-specific methylation of lysine residues in histones that are fundamental in epigenetic regulation of gene activation and silencing in eukaryotic organisms. SET domains appear to be protein-protein interaction domains. It has been demonstrated that SET domains mediate interactions with a family of proteins that display similarity with dual-specificity phosphatases (dsPTPases). A subset of SET domains has been called PR domains. These domains are divergent in sequence from other SET domains, but also appear to mediate protein-protein interaction. The SET domain consists of two regions known as N-SET and C-SET. C-SET forms an unusual and conserved knot-like structure of probable functional importance. In addition to N-SET and C-SET, an insert region (I-SET) and flanking regions of high structural variability form part of the overall structure. Some family members contain a pre-SET domain, which is found in a number of histone methyltransferases (HMTase), and a post-SET domain, which harbors a zinc-binding site.


Pssm-ID: 380914 [Multi-domain]  Cd Length: 72  Bit Score: 55.33  E-value: 5.53e-10
                        10        20        30
                ....*....|....*....|....*....|....*....
6PAF_A      209 NHSCDPNCSIVF----NGPHLLLRAVRDIEVGEELTICY 243
Cdd:cd08161  33 NHSCEPNCEFEEvyvgGKPRVFIVALRDIKAGEELTVDY 71
SET_Suv4-20-like cd10524
SET domain (including post-SET domain) found in Drosophila melanogaster suppressor of ...
 205-271 6.58e-08

SET domain (including post-SET domain) found in Drosophila melanogaster suppressor of variegation 4-20 (Suv4-20) and similar proteins; Suv4-20 (also termed Su(var)4-20) is a histone-lysine N-methyltransferase that specifically trimethylates 'Lys-20' of histone H4. It acts as a dominant suppressor of position-effect variegation. The family also includes Suv4-20 homologs, lysine N-methyltransferase 5B (KMT5B) and lysine N-methyltransferase 5C (KMT5C). Both KMT5B (also termed lysine-specific methyltransferase 5B, or suppressor of variegation 4-20 homolog 1, or Su(var)4-20 homolog 1, or Suv4-20h1) and KMT5C (also termed lysine-specific methyltransferase 5C, or suppressor of variegation 4-20 homolog 2, or Su(var)4-20 homolog 2, or Suv4-20h2) are histone methyltransferases that specifically trimethylate 'Lys-20' of histone H4 (H4K20me3). They play central roles in the establishment of constitutive heterochromatin in pericentric heterochromatin regions.


Pssm-ID: 380922 [Multi-domain]  Cd Length: 141  Bit Score: 51.51  E-value: 6.58e-08
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|....*...
6PAF_A      205 ISLLNHSCDPNCSIVFNGPHL-LLRAVRDIEVGEELTICYLDmlmtseerrKQLRDQYCfECDCFRCQ 271
Cdd:cd10524  77 AAFINHDCRPNCKFVPTGKSTaCVKVLRDIEPGEEITVYYGD---------NYFGENNE-ECECETCE 134
SET_SpSet7-like cd10540
SET domain found in Schizossacharomyces pombe Set7 and similar proteins; Schizosaccharomyces ...
 206-243 8.64e-08

SET domain found in Schizossacharomyces pombe Set7 and similar proteins; Schizosaccharomyces pombe Set7 is a novel histone-lysine N-methyltransferase. The family also includes a viral histone H3 lysine 27 methyltransferase from Paramecium bursaria Chlorella virus 1 (PBCV-1).


Pssm-ID: 380938  Cd Length: 112  Bit Score: 50.33  E-value: 8.64e-08
                        10        20        30        40
                ....*....|....*....|....*....|....*....|
6PAF_A      206 SLLNHSCDPNCSIVFNGPHLLLR--AVRDIEVGEELTICY 243
Cdd:cd10540  67 SMFNHSYTPNAEYEIDFENQTIVfyALRDIEAGEELTINY 106
SET_LSMT cd10527
SET domain found in Rubisco large subunit methyltransferase (LSMT) and similar proteins; ...
 15-243 9.83e-08

SET domain found in Rubisco large subunit methyltransferase (LSMT) and similar proteins; Rubisco LSMT is a non-histone protein methyl transferase responsible for the trimethylation of lysine14 in the large subunit of Rubisco (ribulose-1,5-bisphosphate carboxylase/oxygenase). The family also includes SET domain-containing proteins, SETD3, SETD4 and SETD6, which belong to methyltransferase class VII that represents classical non-histone SET domain methyltransferases. Members in this family contain a SET domain and a C-terminal RubisCO LSMT substrate-binding (Rubis-subs-bind) domain.


Pssm-ID: 380925 [Multi-domain]  Cd Length: 236  Bit Score: 52.45  E-value: 9.83e-08
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
6PAF_A       15 TANRGNGLRAVTPLRPGELLFRSdPLaytvckgsrgvvcdRCLLGKEKLMRCSQCRVAKYCSAKCQKKAWpdhkreckcl 94
Cdd:cd10527   7 SPDGGRGLFATRDIAAGEVLLSV-PR--------------SLLLTVETARESPLGGAALALLELDPELSW---------- 61

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
6PAF_A       95 ksckpryppdSVRLLGRVVFKLMDGA-----------PSESEKLYSFYDLE-------SNINKLTEDKKEGLRQLVMTFQ 156
Cdd:cd10527  62 ----------DVALALFLLYERARGPdsfwapyldslPRPFEDTPLFWSEEeldalqgTPLLEAAAAQRRRLREEYEALV 131

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
6PAF_A      157 HFMREEIQDASQLPPAFDLFE-AFAKVICNSFTIcNAEMQEVGVGLYPSISLLNHSCD-PNCSIVF--NGPHLLLRAVRD 232
Cdd:cd10527 132 EALPEALPAEPGEAFTLEEFLwALALVLSRAFSL-PVPDGGGGLALVPLADMLNHSPDaPNVRYEYdeDEGSFVLVATRD 210

                       250
                ....*....|.
6PAF_A      233 IEVGEELTICY 243
Cdd:cd10527 211 IAAGEEVFISY 221
SET_SETD8 cd10528
SET domain found in SET domain-containing protein 8 (SETD8) and similar proteins; SETD8 (EC 2. ...
 207-245 1.67e-06

SET domain found in SET domain-containing protein 8 (SETD8) and similar proteins; SETD8 (EC 2.1.1.43; also termed N-lysine methyltransferase KMT5A, H4-K20-HMTase KMT5A, lysine N-methyltransferase 5A, lysine-specific methylase 5A, PR/SET domain-containing protein 07, PR-Set7 or PR/SET07) is a nucleosomal histone-lysine N-methyltransferase that specifically monomethylates 'Lys-20' of histone H4 (H4K20me1). It plays a central role in the silencing of euchromatic genes.


Pssm-ID: 380926 [Multi-domain]  Cd Length: 141  Bit Score: 47.19  E-value: 1.67e-06
                        10        20        30        40
                ....*....|....*....|....*....|....*....|....
6PAF_A      207 LLNHSC-DPNC---SIVFNG-PHLLLRAVRDIEVGEELTICYLD 245
Cdd:cd10528  97 LINHSKkKPNLktkLLVIDGvPHLILVAKRDIKPGEELLYDYGD 140
SET_SETD2-like cd10531
SET domain (including post-SET domain) found in SET domain-containing protein 2 (SETD2), ...
 208-243 3.57e-06

SET domain (including post-SET domain) found in SET domain-containing protein 2 (SETD2), nuclear SETD2 (NSD2), ASH1-like protein (ASH1L) and similar proteins; This family includes SET domain-containing protein 2 (SETD2), nuclear SETD2 (NSD2) and ASH1-like protein (ASH1L), which function as histone-lysine N-methyltransferases. SETD2 specifically trimethylates 'Lys-36' of histone H3 (H3K36me3) using demethylated 'Lys-36' (H3K36me2) as substrate. NSD2 shows histone H3 'Lys-27' (H3K27me) methyltransferase activity. ASH1L specifically methylates 'Lys-36' of histone H3 (H3K36me). The family also includes Arabidopsis thaliana ASH1-related protein 3 (ASHR3) and similar proteins.


Pssm-ID: 380929  Cd Length: 136  Bit Score: 46.09  E-value: 3.57e-06
                        10        20        30        40
                ....*....|....*....|....*....|....*....|
6PAF_A      208 LNHSCDPNCS----IVFNGPHLLLRAVRDIEVGEELTICY 243
Cdd:cd10531  77 INHSCEPNCEtqkwIVNGEYRIGIFALRDIPAGEELTFDY 116
SET_SETD1-like cd10518
SET domain (including post-SET domain) found in SET domain-containing proteins (SETD1A/SETD1B), ...
 207-240 4.09e-06

SET domain (including post-SET domain) found in SET domain-containing proteins (SETD1A/SETD1B), histone-lysine N-methyltransferases (KMT2A/KMT2B/KMT2C/KMT2D) and similar proteins; This family includes SET domain-containing protein 1A (SETD1A), 1B (SETD1B), as well as histone-lysine N-methyltransferase 2A (KMT2A), 2B (KMT2B), 2C (KMT2C), 2D (KMT2D). These proteins are histone-lysine N-methyltransferases (EC 2.1.1.43) that specifically methylate 'Lys-4' of histone H3 (H3K4me).


Pssm-ID: 380916  Cd Length: 150  Bit Score: 46.44  E-value: 4.09e-06
                        10        20        30
                ....*....|....*....|....*....|....*...
6PAF_A      207 LLNHSCDPNC---SIVFNGP-HLLLRAVRDIEVGEELT 240
Cdd:cd10518  90 FINHSCDPNCyakIITVDGEkHIVIFAKRDIAPGEELT 127
SET_NSD cd19173
SET domain (including post-SET domain) found in nuclear SET domain-containing proteins, NSD1, ...
 208-250 9.69e-06

SET domain (including post-SET domain) found in nuclear SET domain-containing proteins, NSD1, NSD2, NSD3 and similar proteins; The nuclear receptor-binding SET Domain (NSD) family of histone H3 lysine 36 methyltransferases is comprised of NSD1, NSD2, and NSD3, which are primarily known to be involved in chromatin integrity and gene expression through mono-, di-, or tri-methylating lysine 36 of histone H3 (H3K36), respectively. NSD1 (EC 2.1.1.43; also termed histone-lysine N-methyltransferase H3 lysine-36 and H4 lysine-20 specific, androgen receptor coactivator 267 kDa protein (ARA267), androgen receptor-associated protein of 267 kDa, H3-K36-HMTase, H4-K20-HMTase, lysine N-methyltransferase 3B (KMT3B) or NR-binding SET domain-containing protein 1) functions as a histone-lysine N-methyltransferase that preferentially methylates 'Lys-36' of histone H3 and 'Lys-20' of histone H4. NSD2 (EC 2.1.1.43; also termed multiple myeloma SET domain-containing protein (MMSET), protein trithorax-5 (TRX5), or wolf-Hirschhorn syndrome candidate 1 protein (WHSC1)) acts as histone-lysine N-methyltransferase with histone H3 'Lys-27' (H3K27me) methyltransferase activity. NSD3 (EC 2.1.1.43; also termed protein whistle, WHSC1-like 1 isoform 9 with methyltransferase activity to lysine, Wolf-Hirschhorn syndrome candidate 1-like protein 1 (WHSC1L1), or WHSC1-like protein 1) functions as a histone-lysine N-methyltransferase that preferentially methylates 'Lys-4' and 'Lys-27' of histone H3.


Pssm-ID: 380950 [Multi-domain]  Cd Length: 142  Bit Score: 45.00  E-value: 9.69e-06
                        10        20        30        40
                ....*....|....*....|....*....|....*....|....*...
6PAF_A      208 LNHSCDPNCSI---VFNGPHLL-LRAVRDIEVGEELTICY-LDMLMTS 250
Cdd:cd19173  79 MNHSCQPNCETqkwTVNGDTRVgLFAVRDIPAGEELTFNYnLDCLGNE 126
SET_SETMAR cd10544
SET domain (including pre-SET and post-SET domains) found in SET domain and mariner ...
 208-243 1.05e-05

SET domain (including pre-SET and post-SET domains) found in SET domain and mariner transposase fusion protein (SETMAR) and similar proteins; SETMAR (also termed metnase) is a DNA-binding protein that is indirectly recruited to sites of DNA damage through protein-protein interactions. It has a sequence-specific DNA-binding activity recognizing the 19-mer core of the 5'-terminal inverted repeats (TIRs) of the Hsmar1 element and displays a DNA nicking and end joining activity. SETMAR also acts as a histone-lysine N-methyltransferase that methylates 'Lys-4' and 'Lys-36' of histone H3. It specifically mediates dimethylation of H3 'Lys-36' at sites of DNA double-strand break and may recruit proteins required for efficient DSB repair through non-homologous end-joining.


Pssm-ID: 380942 [Multi-domain]  Cd Length: 254  Bit Score: 46.52  E-value: 1.05e-05
                        10        20        30        40
                ....*....|....*....|....*....|....*....|.
6PAF_A      208 LNHSCDPNCSIV---FNG--PHLLLRAVRDIEVGEELTICY 243
Cdd:cd10544 173 LNHSCEPNLFMVpvrVDSmvPKLALFAARDIVAGEELSFDY 213
SET_ASH1L cd19174
SET domain (including post-SET domain) found in ASH1-like protein (ASH1L) and similar proteins; ...
 209-243 5.59e-05

SET domain (including post-SET domain) found in ASH1-like protein (ASH1L) and similar proteins; ASH1L (EC 2.1.1.43; also termed absent small and homeotic disks protein 1 homolog, KMT2H, or lysine N-methyltransferase 2H) acts as histone-lysine N-methyltransferase that specifically methylates 'Lys-36' of histone H3 (H3K36me). It plays important roles in development; heterozygous mutation of ASH1L is associated with severe intellectual disability (ID) and multiple congenital anomaly (MCA).


Pssm-ID: 380951 [Multi-domain]  Cd Length: 141  Bit Score: 43.05  E-value: 5.59e-05
                        10        20        30
                ....*....|....*....|....*....|....*....
6PAF_A      209 NHSCDPNCSIV---FNG-PHLLLRAVRDIEVGEELTICY 243
Cdd:cd19174  77 NHSCDPNCEMQkwsVNGvYRIGLFALKDIPAGEELTYDY 115
SET_KMT5C cd19185
SET domain (including post-SET domain) found in histone-lysine N-methyltransferase 5C (KMT5C) ...
 206-277 6.82e-05

SET domain (including post-SET domain) found in histone-lysine N-methyltransferase 5C (KMT5C) and similar proteins; KMT5C (also termed lysine N-methyltransferase 5C, lysine-specific methyltransferase 5C, suppressor of variegation 4-20 homolog 2, Su(var)4-20 homolog 2 or Suv4-20h2) is a histone methyltransferase that specifically trimethylates 'Lys-20' of histone H4 (H4K20me3). It plays a central role in the establishment of constitutive heterochromatin in pericentric heterochromatin regions.


Pssm-ID: 380962  Cd Length: 142  Bit Score: 42.72  E-value: 6.82e-05
                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
6PAF_A      206 SLLNHSCDPNCSIV-FNGPHLLLRAVRDIEVGEELTiCYLDMLMTSEerRKQLrdqycfeCDCFRCQTQDKDA 277
Cdd:cd19185  78 AFINHDCKPNCKFVpADGNAACVKVLRDIEPGDEVT-CFYGEGFFGE--KNEH-------CECHTCERKGEGA 140
SET_EZH cd10519
SET domain found in enhancer of zeste homolog 1 (EZH1), zeste homolog 2 (EZH2) and similar ...
 209-243 9.37e-05

SET domain found in enhancer of zeste homolog 1 (EZH1), zeste homolog 2 (EZH2) and similar proteins; The family includes EZH1 and EZH2. EZH1 (EC 2.1.1.43; also termed ENX-2, or histone-lysine N-methyltransferase EZH1) is a catalytic subunit of the PRC2/EED-EZH1 complex, which methylates 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. EZH2 (EC 2.1.1.43; also termed lysine N-methyltransferase 6, ENX-1, or histone-lysine N-methyltransferase EZH2) is a catalytic subunit of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. Both, EZH1 and EZH2, can mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively.


Pssm-ID: 380917  Cd Length: 117  Bit Score: 41.85  E-value: 9.37e-05
                        10        20        30
                ....*....|....*....|....*....|....*....
6PAF_A      209 NHSCDPNCS---IVFNGPHLL-LRAVRDIEVGEELTICY 243
Cdd:cd10519  77 NHSSNPNCYakvMMVNGDHRIgIFAKRDIEAGEELFFDY 115
SET_SETD2 cd19172
SET domain (including post-SET domain) found in SET domain-containing protein 2 (SETD2) and ...
 208-243 9.99e-05

SET domain (including post-SET domain) found in SET domain-containing protein 2 (SETD2) and similar proteins; SETD2 (also termed HIF-1, huntingtin yeast partner B, huntingtin-interacting protein 1 (HIP-1), huntingtin-interacting protein B, lysine N-methyltransferase 3A or protein-lysine N-methyltransferase SETD2) acts as histone-lysine N-methyltransferase that specifically trimethylates 'Lys-36' of histone H3 (H3K36me3) using demethylated 'Lys-36' (H3K36me2) as substrate. It has been shown that methylation is a posttranslational modification of dynamic microtubules and that SETD2 methylates alpha-tubulin at lysine 40, the same lysine that is marked by acetylation on microtubules. Methylation of microtubules occurs during mitosis and cytokinesis and can be ablated by SETD2 deletion, which causes mitotic spindle and cytokinesis defects, micronuclei, and polyploidy.


Pssm-ID: 380949 [Multi-domain]  Cd Length: 142  Bit Score: 42.18  E-value: 9.99e-05
                        10        20        30        40
                ....*....|....*....|....*....|....*....|...
6PAF_A      208 LNHSCDPNCSI---VFNGphlLLR----AVRDIEVGEELTICY 243
Cdd:cd19172  79 INHSCEPNCETqkwTVNG---ELRvgffAKRDIPAGEELTFDY 118
SET_SUV39H cd10542
SET domain (including pre-SET and post-SET domains) found in suppressor of variegation 3-9 ...
 196-252 1.15e-04

SET domain (including pre-SET and post-SET domains) found in suppressor of variegation 3-9 homologs, SUV39H1, SUV39H2 and similar proteins; This family includes SUV39H1 (also termed histone H3-K9 methyltransferase 1, H3-K9-HMTase 1, lysine N-methyltransferase 1A, KMT1A, position-effect variegation 3-9 homolog, SUV39H, or Su(var)3-9 homolog 1) and SUV39H2 (also termed histone H3-K9 methyltransferase 2, H3-K9-HMTase 2, lysine N-methyltransferase 1B, KMT1B, or Su(var)3-9 homolog 2), both act as histone-lysine N-methyltransferases that specifically trimethylate 'Lys-9' of histone H3 (H3K9me3) using monomethylated H3 'Lys-9' as substrate. They mainly function in heterochromatin regions, thereby playing central roles in the establishment of constitutive heterochromatin at pericentric and telomere regions. Also included are Schizosaccharomyces pombe H3K9 methyltransferase Clr4 (SUV39H homolog) and Neurospora crassa DIM-5, both of which also methylate 'Lys-9' of histone H3.


Pssm-ID: 380940 [Multi-domain]  Cd Length: 245  Bit Score: 43.44  E-value: 1.15e-04
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|....*.
6PAF_A      196 EVGVGLYPSIS-LLNHSCDPNCSI--VFNG------PHLLLRAVRDIEVGEELTicyLDMLMTSEE 252
Cdd:cd10542 155 TVDAAYYGNIShFINHSCDPNLAVyaVWINhldprlPRIAFFAKRDIKAGEELT---FDYLMTGTG 217
SET_SETD4 cd19177
SET domain found in SET domain-containing protein 4 (SETD4) and similar proteins; SETD4 is a ...
 201-243 1.50e-04

SET domain found in SET domain-containing protein 4 (SETD4) and similar proteins; SETD4 is a cytosolic and nuclear functional lysine methyltransferase that plays a crucial role in breast carcinogenesis. However, its specific substrates and modification sites remain to be disclosed.


Pssm-ID: 380954 [Multi-domain]  Cd Length: 245  Bit Score: 43.06  E-value: 1.50e-04
                        10        20        30        40
                ....*....|....*....|....*....|....*....|....*
6PAF_A      201 LYPSISLLNHSCDPNCSIVFN--GPHLLLRAVRDIEVGEELTICY 243
Cdd:cd19177 185 LAPFLDLLNHSPDVNVKAGFNksGKCYEIRTGTDYKKGEEVFISY 229
SET_KMT2A_2B cd19170
SET domain (including post-SET domain) found in histone-lysine N-methyltransferase 2A (KMT2A), ...
 208-243 3.17e-04

SET domain (including post-SET domain) found in histone-lysine N-methyltransferase 2A (KMT2A), 2B (KMT2B) and similar proteins; This family includes KMT2A and KMT2B. Both KMT2A (also termed ALL-1 or CXXC7 or MLL or MLL1 or TRX1 or HRX) and KMT2B (also termed MLL4 or TRX2) act as histone methyltransferases that methylate 'Lys-4' of histone H3 (H3K4me).


Pssm-ID: 380947 [Multi-domain]  Cd Length: 152  Bit Score: 40.84  E-value: 3.17e-04
                        10        20        30        40
                ....*....|....*....|....*....|....*....|
6PAF_A      208 LNHSCDPNCSI----VFNGPHLLLRAVRDIEVGEELTICY 243
Cdd:cd19170  90 INHSCEPNCYSrvvnIDGKKHIVIFALRRILRGEELTYDY 129
SET_SUV39H_DIM5-like cd19473
SET domain (including pre-SET domain) found in Neurospora crassa (DIM-5) and similar proteins; ...
 209-267 6.28e-04

SET domain (including pre-SET domain) found in Neurospora crassa (DIM-5) and similar proteins; This subfamily contains Neurospora crassa DIM-5 (also termed H3-K9-HMTase dim-5, or HKMT) which functions as histone-lysine N-methyltransferase that specifically trimethylates histone H3 to form H3K9me3.


Pssm-ID: 380996 [Multi-domain]  Cd Length: 274  Bit Score: 41.53  E-value: 6.28e-04
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....
6PAF_A      209 NHSCDPNCSIvFN--GPH-------LLLRAVRDIEVGEELTICYLDMLMTSEERRK-QLRDQYCFECDC 267
Cdd:cd19473 198 NHSCDPNLRI-FArvGDHadkhihdLAFFAIKDIPRGTELTFDYVDGVTGLDDDAGdEEKEKEMTKCLC 265
SET_LegAS4-like cd10522
SET domain found in Legionella pneumophila type IV secretion system effector LegAS4 and ...
 209-243 7.61e-04

SET domain found in Legionella pneumophila type IV secretion system effector LegAS4 and similar proteins; LegAS4 is a type IV secretion system effector of Legionella pneumophila. It contains a SET domain that is involved in the modification of Lys4 of histone H3 (H3K4) in the nucleolus of the host cell, thereby enhancing heterochromatic rDNA transcription. It also contains an ankyrin repeat domain of unknown function at its C-terminal region.


Pssm-ID: 380920 [Multi-domain]  Cd Length: 122  Bit Score: 39.25  E-value: 7.61e-04
                        10        20        30
                ....*....|....*....|....*....|....*....
6PAF_A      209 NHSCDPNCSIVF---NG-PHLLLRAVRDIEVGEELTICY 243
Cdd:cd10522  78 NHSDQPNLELIVrtlKGeQHIGFVAIRDIKPGEELFISY 116
SET_SETDB-like cd10538
SET domain (including pre-SET and post-SET domains) found in SET domain bifurcated 1 (SETDB1) ...
 208-243 9.27e-04

SET domain (including pre-SET and post-SET domains) found in SET domain bifurcated 1 (SETDB1) and 2 (SETDB2), suppressor of variegation 3-9 homologs, SUV39H1 and SUV39H2, euchromatic histone-lysine N-methyltransferase EHMT1 and EHMT2, and similar proteins; The family includes SET domain bifurcated 1 (SETDB1) and 2 (SETDB2), suppressor of variegation 3-9 homologs, SUV39H1 and SUV39H2, euchromatic histone-lysine N-methyltransferase EHMT1 and EHMT2. SETDB1 (EC 2.1.1.43; also termed ERG-associated protein with SET domain (ESET), histone H3-K9 methyltransferase 4, H3-K9-HMTase 4, or lysine N-methyltransferase 1E (KMT1E)) acts as a histone-lysine N-methyltransferase that specifically trimethylates 'Lys-9' of histone H3 (H3K9me3). It mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. SETDB2 (EC 2.1.1.43; also termed chronic lymphocytic leukemia deletion region gene 8 protein (CLLD8), or lysine N-methyltransferase 1F (KMT1F)) acts as a histone-lysine N-methyltransferase that specifically trimethylates 'Lys-9' of histone H3 (H3K9me3). It is involved in left-right axis specification in early development and mitosis. SUV39H1 (also termed histone H3-K9 methyltransferase 1, H3-K9-HMTase 1, lysine N-methyltransferase 1A, KMT1A, position-effect variegation 3-9 homolog, SUV39H, or Su(var)3-9 homolog 1) and SUV39H2 (also termed histone H3-K9 methyltransferase 2, H3-K9-HMTase 2, lysine N-methyltransferase 1B, KMT1B, or Su(var)3-9 homolog 2), both act as histone-lysine N-methyltransferases that specifically trimethylate 'Lys-9' of histone H3 (H3K9me3) using monomethylated H3 'Lys-9' as substrate. They mainly function in heterochromatin regions, thereby playing central roles in the establishment of constitutive heterochromatin at pericentric and telomere regions. EHMT1 (also termed Eu-HMTase1, G9a-like protein 1, GLP, GLP1, histone H3-K9 methyltransferase 5, H3-K9-HMTase 5, lysine N-methyltransferase 1D, or KMT1D) and EHMT2 (also termed Eu-HMTase2, HLA-B-associated transcript 8, histone H3-K9 methyltransferase 3, H3-K9-HMTase 3, lysine N-methyltransferase 1C, KMT1C, or protein G9a), both act as histone-lysine N-methyltransferases that specifically mono- and dimethylate 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. This family also includes the pre-SET domain, which is found in a number of histone methyltransferases (HMTase), N-terminal to the SET domain. Pre-SET domain is a zinc binding motif which contains 9 conserved cysteines that coordinate three zinc ions. It is thought that this region plays a structural role in stabilizing SET domains. Most family members, except for Arabidopsis thaliana SUVH9, contain a post-SET domain which harbors a zinc-binding site.


Pssm-ID: 380936 [Multi-domain]  Cd Length: 217  Bit Score: 40.43  E-value: 9.27e-04
                        10        20        30        40
                ....*....|....*....|....*....|....*....|....
6PAF_A      208 LNHSCDPN---CSIVFNG-----PHLLLRAVRDIEVGEELTICY 243
Cdd:cd10538 173 INHSCDPNlfpFNVVIDHddlryPRIALFATRDILPGEELTFDY 216
SET_SpSET3-like cd19183
SET domain (including post-SET domain) found in Schizosaccharomyces pombe SET ...
 210-241 9.32e-04

SET domain (including post-SET domain) found in Schizosaccharomyces pombe SET domain-containing protein 3 (SETD3) and similar proteins; Schizosaccharomyces pombe SETD3 functions as a transcriptional regulator that acts via the formation of large multiprotein complexes that modify and/or remodel the chromatin. It is required for both, gene activation and repression.


Pssm-ID: 380960  Cd Length: 173  Bit Score: 40.08  E-value: 9.32e-04
                        10        20        30
                ....*....|....*....|....*....|....*...
6PAF_A      210 HSCDPNCSIV----FNG--PHLLLRAVRDIEVGEELTI 241
Cdd:cd19183  83 RSCRPNAELVtvasDSGsvLKFVLYASRDISPGEEITI 120
SET_KMT2C_2D cd19171
SET domain (including post-SET domain) found in histone-lysine N-methyltransferase 2C (KMT2C), ...
 208-243 1.06e-03

SET domain (including post-SET domain) found in histone-lysine N-methyltransferase 2C (KMT2C), 2D (KMT2D) and similar proteins; This family includes KMT2C and KMT2D. Both, KMT2C (also termed HALR or MLL3) and KMT2D (also termed ALR or MLL2), act as histone methyltransferases that methylate 'Lys-4' of histone H3 (H3K4me). They are subunits of MLL2/3 complex, a coactivator complex of nuclear receptors, involved in transcriptional coactivation.


Pssm-ID: 380948 [Multi-domain]  Cd Length: 153  Bit Score: 39.34  E-value: 1.06e-03
                        10        20        30        40
                ....*....|....*....|....*....|....*....|
6PAF_A      208 LNHSCDPNCS---IVF-NGPHLLLRAVRDIEVGEELTICY 243
Cdd:cd19171  90 INHSCNPNCVaevVTFdKEKKIIIISNRRIAKGEELTYDY 129
SET_SET1 cd20072
SET domain (including post-SET domain) found in catalytic component of the Saccharomyces ...
 207-243 1.35e-03

SET domain (including post-SET domain) found in catalytic component of the Saccharomyces cerevisiae COMPASS complex and similar proteins; The family contains mostly fungal SET domains, including SET1 found in the catalytic component of the Saccharomyces cerevisiae COMPASS (complex of proteins associated with Set1). SET1 is a histone-lysine N-methyltransferase that specifically methylates 'Lys-4' of histone H3 (H3K4me), when part of the SET1 histone methyltransferase (HMT) complex. The activity of this catalytic domain is established through forming a complex with a set of core proteins; it is extensively contacted by Cps60 (Bre2), Cps50 (Swd1), and Cps30 (Swd3).


Pssm-ID: 380998  Cd Length: 148  Bit Score: 38.94  E-value: 1.35e-03
                        10        20        30        40
                ....*....|....*....|....*....|....*....|.
6PAF_A      207 LLNHSCDPNCS---IVFNGPH-LLLRAVRDIEVGEELTICY 243
Cdd:cd20072  89 FINHCCDPNCTakiIKVEGEKrIVIYAKRDIAAGEELTYDY 129
SET_NSD2 cd19211
SET domain (including post-SET domain) found in nuclear SET domain-containing protein 2 (NSD2) ...
 207-247 2.26e-03

SET domain (including post-SET domain) found in nuclear SET domain-containing protein 2 (NSD2) and similar proteins; NSD2 (EC 2.1.1.43; also termed multiple myeloma SET domain-containing protein (MMSET), protein trithorax-5 (TRX5), or wolf-Hirschhorn syndrome candidate 1 protein (WHSC1)) acts as histone-lysine N-methyltransferase with histone H3 'Lys-36' (H3K36me) methyltransferase activity. NSD2 has been shown to mediate di- and trimethylation of H3K36 and dimethylation of H4K20 in different systems, and has been characterized as a transcriptional repressor interacting with histone deacetylase HDAC1 and histone demethylase LSD1. NSD2 mediates constitutive NF-kappaB signaling for cancer cell proliferation, survival and tumor growth. It is highly overexpressed in several types of human cancers, including small-cell lung cancers, neuroblastoma, carcinomas of stomach and colon, and bladder cancers, and its overexpression tends to be associated with tumor aggressiveness. WHSC1 is frequently deleted in Wolf-Hirschhorn syndrome (WHS).


Pssm-ID: 380988 [Multi-domain]  Cd Length: 142  Bit Score: 38.43  E-value: 2.26e-03
                        10        20        30        40
                ....*....|....*....|....*....|....*....|....*.
6PAF_A      207 LLNHSCDPNCSI---VFNG-PHLLLRAVRDIEVGEELTICY-LDML 247
Cdd:cd19211  78 FMNHSCQPNCETqkwTVNGdTRVGLFAVCDIPAGTELTFNYnLDCL 123
SET_Suv4-20 cd19186
SET domain (including post-SET domain) found in Drosophila melanogaster suppressor of ...
 206-277 3.56e-03

SET domain (including post-SET domain) found in Drosophila melanogaster suppressor of variegation 4-20 (Suv4-20) and similar proteins; Suv4-20 (also termed Su(var)4-20) is a histone-lysine N-methyltransferase that specifically trimethylates 'Lys-20' of histone H4. It acts as a dominant suppressor of position-effect variegation.


Pssm-ID: 380963  Cd Length: 142  Bit Score: 37.82  E-value: 3.56e-03
                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
6PAF_A      206 SLLNHSCDPNCSIVFNGPHL-LLRAVRDIEVGEELTICYLDMLMTseerrkqlrDQYCFeCDCFRCQTQDKDA 277
Cdd:cd19186  79 AFINHDCRPNCKFVSTGRDTaCVQVLRDIEPGEEITCFYGEDFFG---------DNNCY-CECETCERRQTGA 141
SET_KMT2A cd19206
SET domain (including post-SET domain) found in histone-lysine N-methyltransferase 2A (KMT2A) ...
 204-243 4.73e-03

SET domain (including post-SET domain) found in histone-lysine N-methyltransferase 2A (KMT2A) and similar proteins; KMT2A (EC2.1.1.43; also termed lysine N-methyltransferase 2A, ALL-1, CXXC-type zinc finger protein 7 (CXXC7), myeloid/lymphoid or mixed-lineage leukemia (MLL), myeloid/lymphoid or mixed-lineage leukemia protein 1 (MLL1), trithorax-like protein (TRX1), or zinc finger protein HRX) acts as a histone methyltransferase that plays an essential role in early development and hematopoiesis. It is a catalytic subunit of the MLL1/MLL complex, a multiprotein complex that mediates both methylation of 'Lys-4' of histone H3 (H3K4me) complex and acetylation of 'Lys-16' of histone H4 (H4K16ac).


Pssm-ID: 380983 [Multi-domain]  Cd Length: 154  Bit Score: 37.69  E-value: 4.73e-03
                        10        20        30        40
                ....*....|....*....|....*....|....*....|....
6PAF_A      204 SISLLNHSCDPNC-SIVFN---GPHLLLRAVRDIEVGEELTICY 243
Cdd:cd19206  86 AARFINHSCEPNCySRVINidgQKHIVIFAMRKIYRGEELTYDY 129
SET_ASHR3-like cd19175
SET domain (including post-SET domain) found in Arabidopsis thaliana ASH1-related protein 3 ...
 208-243 5.63e-03

SET domain (including post-SET domain) found in Arabidopsis thaliana ASH1-related protein 3 (ASHR3) and similar proteins; This family includes Arabidopsis thaliana ASH1-related protein 3 (ASHR3, also termed protein SET DOMAIN GROUP 4 or protein stamen loss), ASH1 homolog 3 (ASHH3, also termed protein SET DOMAIN GROUP 7) and homolog 4 (ASHH4, also termed protein SET DOMAIN GROUP 24). They all function as histone-lysine N-methyltransferases (EC 2.1.1.43).


Pssm-ID: 380952 [Multi-domain]  Cd Length: 139  Bit Score: 37.01  E-value: 5.63e-03
                        10        20        30        40
                ....*....|....*....|....*....|....*....|
6PAF_A      208 LNHSCDPNCS----IVFNGPHLLLRAVRDIEVGEELTICY 243
Cdd:cd19175  77 INHSCDPNCElqkwQVDGETRIGVFAIRDIKKGEELTYDY 116
SET_KMT5B cd19184
SET domain (including post-SET domain) found in histone-lysine N-methyltransferase 5B (KMT5B) ...
 206-277 6.35e-03

SET domain (including post-SET domain) found in histone-lysine N-methyltransferase 5B (KMT5B) and similar proteins; KMT5B (also termed lysine N-methyltransferase 5B, lysine-specific methyltransferase 5B, suppressor of variegation 4-20 homolog 1, Su(var)4-20 homolog 1 or Suv4-20h1) is a histone methyltransferase that specifically trimethylates 'Lys-20' of histone H4 (H4K20me3). It plays a central role in the establishment of constitutive heterochromatin in pericentric heterochromatin regions.


Pssm-ID: 380961  Cd Length: 144  Bit Score: 36.94  E-value: 6.35e-03
                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
6PAF_A      206 SLLNHSCDPNCSIVFNGPHL-LLRAVRDIEVGEELTICYLDMLMTSEerrkqlrDQYcfeCDCFRCQTQDKDA 277
Cdd:cd19184  78 AFINHDCRPNCKFVSTGRDTaCVKALRDIEPGEEISCYYGDGFFGEN-------NEF---CECYTCERRGTGA 140
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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