N-terminal domain of Kruppel-like factor 10; Kruppel-like factor 10 (KLF10; also known as Krueppel-like factor 10; early growth response(EGR)-alpha/EGRA; TGFbeta inducible early gene-1/TIEG1) is a protein that in humans is encoded by the KLF10 gene. KLF10 was first identified in human osteoblasts and plays a role in mediating estrogen (E2) signaling in bone and skeletal homeostasis and a regulatory role in tumor formation and metastasis. It may also play a role in adipocyte differentiation and adipose tissue function. KLF9, KLF10, KLF11, KLF13, KLF14, and KLF16 share a conserved a-helical motif AA/VXXL that mediates their binding to Sin3A and their activities as transcriptional repressors. KLF10 belongs to a family of proteins, called the Specificity Protein (SP)/KLF family, characterized by a C-terminal DNA-binding domain of 81 amino acids consisting of three Kruppel-like C2H2 zinc fingers. These factors bind to a loose consensus motif, namely NNRCRCCYY (where N is any nucleotide; R is A/G, and Y is C/T), such as the recurring motifs in GC and GT boxes (5'-GGGGCGGGG-3' and 5-GGTGTGGGG-3') that are present in promoters and more distal regulatory elements of mammalian genes. Members of the KLF family can act as activators or repressors of transcription depending on cell and promoter context. KLFs regulate various cellular functions, such as proliferation, differentiation, and apoptosis, as well as the development and homeostasis of several types of tissue. In addition to the C-terminal DNA-binding domain, each KLF also has a unique N-terminal activation/repression domain that confers specificity and allows it to bind specifically to a certain partner, leading to distinct activities in vivo. This model represents the N-terminal domain of KLF10.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 3523146   23 AEKSDFEAVEALMSMSCSWKSDFKKYVENRPVTPVSDLSEEEnLLPGTPDFHTIPAFCLTPPYSPSDFEPSQVSNLMAPA 102
Cdd:cd21572   1 MGAGDMEAVEALMSMTKHWKTRSFRLRHFRPLTPSSDSSEDD-DLPSPADFHDSPPFCMTPPYSPPHFEATHPPSAATLH 79

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 3523146  103 PSTVHfkslsdtakphiaaPFKEEEKSPVSAPKLPKAQATSVIRHTADAQLCNHQTCPmkaasilnyqnnsfrrrthlnv 182
Cdd:cd21572  80 PPAAQ--------------PPEEQHLSAETAASQQRFQCTSVIRHTADAQPCSCSSCP---------------------- 123

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 3523146  183 eaarknipcaavspnrskcerntvadvdekasaalydfsvpssetvicrsqpapvspqQKSVLVSPPAVSAGGVPPMPVI 262
Cdd:cd21572 124 ----------------------------------------------------------SSPSVVPSVPAGVAGVSPVPVY 145

                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 3523146  263 CQMVPLPANNP-VVTTVVPSTPPSQPPAVCPPVVFMGTQVPKGAVMFVVPQPVVQS--SKPPVVSPNGTRLSPIAPAPGF 339
Cdd:cd21572 146 CQILPVSSSSTtVVAAQAPLPQPQQQAASPAQVFLMGGQVPKGPVMFLVPQPVVPTlyVQPTLVTPGGTKLAAIAPAPGH 225

                       330       340
                ....*....|....*....|
gi 3523146  340 SPSAA-KVTPQIDSSRIRSH 358
Cdd:cd21572 226 TPSEQrKSPPQPEVSRVRSH 245

FOG: Zn-finger [General function prediction only];

                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|.
gi 3523146  363 PGCGKTYFKSSHLKAHTRTHTGEKPFSCSWKGCERRFARSDELSRHRRTHT 413
Cdd:COG5048  37 PNCTDSFSRLEHLTRHIRSHTGEKPSQCSYSGCDKSFSRPLELSRHLRTHH 87

N-terminal domain of Kruppel-like factor 10; Kruppel-like factor 10 (KLF10; also known as Krueppel-like factor 10; early growth response(EGR)-alpha/EGRA; TGFbeta inducible early gene-1/TIEG1) is a protein that in humans is encoded by the KLF10 gene. KLF10 was first identified in human osteoblasts and plays a role in mediating estrogen (E2) signaling in bone and skeletal homeostasis and a regulatory role in tumor formation and metastasis. It may also play a role in adipocyte differentiation and adipose tissue function. KLF9, KLF10, KLF11, KLF13, KLF14, and KLF16 share a conserved a-helical motif AA/VXXL that mediates their binding to Sin3A and their activities as transcriptional repressors. KLF10 belongs to a family of proteins, called the Specificity Protein (SP)/KLF family, characterized by a C-terminal DNA-binding domain of 81 amino acids consisting of three Kruppel-like C2H2 zinc fingers. These factors bind to a loose consensus motif, namely NNRCRCCYY (where N is any nucleotide; R is A/G, and Y is C/T), such as the recurring motifs in GC and GT boxes (5'-GGGGCGGGG-3' and 5-GGTGTGGGG-3') that are present in promoters and more distal regulatory elements of mammalian genes. Members of the KLF family can act as activators or repressors of transcription depending on cell and promoter context. KLFs regulate various cellular functions, such as proliferation, differentiation, and apoptosis, as well as the development and homeostasis of several types of tissue. In addition to the C-terminal DNA-binding domain, each KLF also has a unique N-terminal activation/repression domain that confers specificity and allows it to bind specifically to a certain partner, leading to distinct activities in vivo. This model represents the N-terminal domain of KLF10.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 3523146   23 AEKSDFEAVEALMSMSCSWKSDFKKYVENRPVTPVSDLSEEEnLLPGTPDFHTIPAFCLTPPYSPSDFEPSQVSNLMAPA 102
Cdd:cd21572   1 MGAGDMEAVEALMSMTKHWKTRSFRLRHFRPLTPSSDSSEDD-DLPSPADFHDSPPFCMTPPYSPPHFEATHPPSAATLH 79

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 3523146  103 PSTVHfkslsdtakphiaaPFKEEEKSPVSAPKLPKAQATSVIRHTADAQLCNHQTCPmkaasilnyqnnsfrrrthlnv 182
Cdd:cd21572  80 PPAAQ--------------PPEEQHLSAETAASQQRFQCTSVIRHTADAQPCSCSSCP---------------------- 123

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 3523146  183 eaarknipcaavspnrskcerntvadvdekasaalydfsvpssetvicrsqpapvspqQKSVLVSPPAVSAGGVPPMPVI 262
Cdd:cd21572 124 ----------------------------------------------------------SSPSVVPSVPAGVAGVSPVPVY 145

                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 3523146  263 CQMVPLPANNP-VVTTVVPSTPPSQPPAVCPPVVFMGTQVPKGAVMFVVPQPVVQS--SKPPVVSPNGTRLSPIAPAPGF 339
Cdd:cd21572 146 CQILPVSSSSTtVVAAQAPLPQPQQQAASPAQVFLMGGQVPKGPVMFLVPQPVVPTlyVQPTLVTPGGTKLAAIAPAPGH 225

                       330       340
                ....*....|....*....|
gi 3523146  340 SPSAA-KVTPQIDSSRIRSH 358
Cdd:cd21572 226 TPSEQrKSPPQPEVSRVRSH 245

Zinc finger, C2H2 type; The C2H2 zinc finger is the classical zinc finger domain. The two conserved cysteines and histidines co-ordinate a zinc ion. The following pattern describes the zinc finger. #-X-C-X(1-5)-C-X3-#-X5-#-X2-H-X(3-6)-[H/C] Where X can be any amino acid, and numbers in brackets indicate the number of residues. The positions marked # are those that are important for the stable fold of the zinc finger. The final position can be either his or cys. The C2H2 zinc finger is composed of two short beta strands followed by an alpha helix. The amino terminal part of the helix binds the major groove in DNA binding zinc fingers. The accepted consensus binding sequence for Sp1 is usually defined by the asymmetric hexanucleotide core GGGCGG but this sequence does not include, among others, the GAG (=CTC) repeat that constitutes a high-affinity site for Sp1 binding to the wt1 promoter.

                          10        20
                  ....*....|....*....|...
gi 3523146    418 FACPMCDRRFMRSDHLTKHARRH 440
Cdd:pfam00096   1 YKCPDCGKSFSRKSNLKRHLRTH 23

Family of unknown function (DUF5585); This is a family of unknown function found in chordata.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 3523146     82 TPPYSPSDFEPSQVSNLMAPAPSTVhfKSLSDTAKPHIAAPFKEEEKSPVSApkLPKAQATSVIRHTADAQLCNHQTCPM 161
Cdd:pfam17823  99 EPATREGAADGAASRALAAAASSSP--SSAAQSLPAAIAALPSEAFSAPRAA--ACRANASAAPRAAIAAASAPHAASPA 174

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 3523146    162 KAASILNYQNNSFRRRTHLNVEAARKNIPcAAVSPNRSKCErNTVADVDEKASAALYdfSVPSSetvicrsQPAPVSPQQ 241
Cdd:pfam17823 175 PRTAASSTTAASSTTAASSAPTTAASSAP-ATLTPARGIST-AATATGHPAAGTALA--AVGNS-------SPAAGTVTA 243

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 3523146    242 KSVLVSPPAVSAGGVPPMPVICQMVPLPANNPVVTTVVPSTPPSQPPAVCPPVVFMGTQVPKGAVMFVVPQPVVQSSKPP 321
Cdd:pfam17823 244 AVGTVTPAALATLAAAAGTVASAAGTINMGDPHARRLSPAKHMPSDTMARNPAAPMGAQAQGPIIQVSTDQPVHNTAGEP 323

                         250       260
                  ....*....|....*....|....*.
gi 3523146    322 VVSPNGTRLSPIAPAPGFSPSAAKVT 347
Cdd:pfam17823 324 TPSPSNTTLEPNTPKSVASTNLAVVT 349

N-terminal domain of Kruppel-like factor 10; Kruppel-like factor 10 (KLF10; also known as Krueppel-like factor 10; early growth response(EGR)-alpha/EGRA; TGFbeta inducible early gene-1/TIEG1) is a protein that in humans is encoded by the KLF10 gene. KLF10 was first identified in human osteoblasts and plays a role in mediating estrogen (E2) signaling in bone and skeletal homeostasis and a regulatory role in tumor formation and metastasis. It may also play a role in adipocyte differentiation and adipose tissue function. KLF9, KLF10, KLF11, KLF13, KLF14, and KLF16 share a conserved a-helical motif AA/VXXL that mediates their binding to Sin3A and their activities as transcriptional repressors. KLF10 belongs to a family of proteins, called the Specificity Protein (SP)/KLF family, characterized by a C-terminal DNA-binding domain of 81 amino acids consisting of three Kruppel-like C2H2 zinc fingers. These factors bind to a loose consensus motif, namely NNRCRCCYY (where N is any nucleotide; R is A/G, and Y is C/T), such as the recurring motifs in GC and GT boxes (5'-GGGGCGGGG-3' and 5-GGTGTGGGG-3') that are present in promoters and more distal regulatory elements of mammalian genes. Members of the KLF family can act as activators or repressors of transcription depending on cell and promoter context. KLFs regulate various cellular functions, such as proliferation, differentiation, and apoptosis, as well as the development and homeostasis of several types of tissue. In addition to the C-terminal DNA-binding domain, each KLF also has a unique N-terminal activation/repression domain that confers specificity and allows it to bind specifically to a certain partner, leading to distinct activities in vivo. This model represents the N-terminal domain of KLF10.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 3523146   23 AEKSDFEAVEALMSMSCSWKSDFKKYVENRPVTPVSDLSEEEnLLPGTPDFHTIPAFCLTPPYSPSDFEPSQVSNLMAPA 102
Cdd:cd21572   1 MGAGDMEAVEALMSMTKHWKTRSFRLRHFRPLTPSSDSSEDD-DLPSPADFHDSPPFCMTPPYSPPHFEATHPPSAATLH 79

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 3523146  103 PSTVHfkslsdtakphiaaPFKEEEKSPVSAPKLPKAQATSVIRHTADAQLCNHQTCPmkaasilnyqnnsfrrrthlnv 182
Cdd:cd21572  80 PPAAQ--------------PPEEQHLSAETAASQQRFQCTSVIRHTADAQPCSCSSCP---------------------- 123

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 3523146  183 eaarknipcaavspnrskcerntvadvdekasaalydfsvpssetvicrsqpapvspqQKSVLVSPPAVSAGGVPPMPVI 262
Cdd:cd21572 124 ----------------------------------------------------------SSPSVVPSVPAGVAGVSPVPVY 145

                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 3523146  263 CQMVPLPANNP-VVTTVVPSTPPSQPPAVCPPVVFMGTQVPKGAVMFVVPQPVVQS--SKPPVVSPNGTRLSPIAPAPGF 339
Cdd:cd21572 146 CQILPVSSSSTtVVAAQAPLPQPQQQAASPAQVFLMGGQVPKGPVMFLVPQPVVPTlyVQPTLVTPGGTKLAAIAPAPGH 225

                       330       340
                ....*....|....*....|
gi 3523146  340 SPSAA-KVTPQIDSSRIRSH 358
Cdd:cd21572 226 TPSEQrKSPPQPEVSRVRSH 245

N-terminal domain of Kruppel-like factor 11; Kruppel-like factor 11 (KLF11; also known as Krueppel-like factor 11; Fetal Kruppel-like factor-1/FKLF-1; maturity-onset diabetes of the young 7/MODY7; TGFbeta Inducible Early Growth Response 2/TIEG2) is a protein that in humans is encoded by the KLF11 gene. KLF11 is involved in cell growth, apoptosis, cellular inflammation and differentiation, endometriosis, and cholesterol, prostaglandin, neurotransmitter, fat, and sugar metabolism. KLF9, KLF10, KLF11, KLF13, KLF14, and KLF16 share a conserved a-helical motif AA/VXXL that mediates their binding to Sin3A and their activities as transcriptional repressors. KLF11 belongs to a family of proteins, called the Specificity Protein (SP)/KLF family, characterized by a C-terminal DNA-binding domain of 81 amino acids consisting of three Kruppel-like C2H2 zinc fingers. These factors bind to a loose consensus motif, namely NNRCRCCYY (where N is any nucleotide; R is A/G, and Y is C/T), such as the recurring motifs in GC and GT boxes (5'-GGGGCGGGG-3' and 5-GGTGTGGGG-3') that are present in promoters and more distal regulatory elements of mammalian genes. Members of the KLF family can act as activators or repressors of transcription depending on cell and promoter context. KLFs regulate various cellular functions, such as proliferation, differentiation, and apoptosis, as well as the development and homeostasis of several types of tissue. In addition to the C-terminal DNA-binding domain, each KLF also has a unique N-terminal activation/repression domain that confers specificity and allows it to bind specifically to a certain partner, leading to distinct activities in vivo. This model represents the N-terminal domain of KLF11.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 3523146   24 EKSDFEAVEALMSMScSW--KSDFKKYVENRPVTPVSDLSEEENLLPGTP----DFHTIPAFCLTPPYSPSDFEPSqvsn 97
Cdd:cd21584   2 EQNDLEAVEALVCMS-SWgqRSQKGDLLKIRPLTPASDSCDSLTLHPAAPelpkDFHSLSSLCMTPPHSPSFAEPS---- 76

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 3523146   98 lmapapstvhfkslsdtakphiaapfkeeeksPVSAPKLPKAQATSVIRHTADAQLCnhqtcpmkaasilnyqnnsfrrr 177
Cdd:cd21584  77 --------------------------------TTAPPPPCRAMATSVIRHTADSSPP----------------------- 101

                       170       180       190       200       210       220       230       240
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 3523146  178 thlnveaarknipcaavspnrskcerntvadvdekasaalydFSVPSSeTVICRSQPAPVSPQQKSVLVSPPAVSAGGVP 257
Cdd:cd21584 102 ------------------------------------------VPVPSP-PVLCQMIPVSGQSGMISAFLQPPALSAGTVK 138

                       250       260       270       280       290       300       310       320
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 3523146  258 PMPvicqmvplpannpvvttvvPSTPPSQPPavcppvVFMGTQVPKGAVMFVVPQPVV---QSSKPPVVSPNGTRLSPIA 334
Cdd:cd21584 139 PIL-------------------PQTAPASQP------LLVGSPVPQGTVMLVLPQASVpqpPQCPQTVMTLGNTKLLPLA 193

                       330       340
                ....*....|....*....|..
gi 3523146  335 PAPGFSPSAAKVTPQIDSSRIR 356
Cdd:cd21584 194 PAPVFIPSGQSCAPQVDFSRRR 215

Zinc finger, C2H2 type; The C2H2 zinc finger is the classical zinc finger domain. The two conserved cysteines and histidines co-ordinate a zinc ion. The following pattern describes the zinc finger. #-X-C-X(1-5)-C-X3-#-X5-#-X2-H-X(3-6)-[H/C] Where X can be any amino acid, and numbers in brackets indicate the number of residues. The positions marked # are those that are important for the stable fold of the zinc finger. The final position can be either his or cys. The C2H2 zinc finger is composed of two short beta strands followed by an alpha helix. The amino terminal part of the helix binds the major groove in DNA binding zinc fingers. The accepted consensus binding sequence for Sp1 is usually defined by the asymmetric hexanucleotide core GGGCGG but this sequence does not include, among others, the GAG (=CTC) repeat that constitutes a high-affinity site for Sp1 binding to the wt1 promoter.

                          10        20
                  ....*....|....*....|...
gi 3523146    418 FACPMCDRRFMRSDHLTKHARRH 440
Cdd:pfam00096   1 YKCPDCGKSFSRKSNLKRHLRTH 23

FOG: Zn-finger [General function prediction only];

                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|.
gi 3523146  363 PGCGKTYFKSSHLKAHTRTHTGEKPFSCSWKGCERRFARSDELSRHRRTHT 413
Cdd:COG5048  37 PNCTDSFSRLEHLTRHIRSHTGEKPSQCSYSGCDKSFSRPLELSRHLRTHH 87

DNA polymerase III subunits gamma and tau; Provisional

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 3523146   232 SQPAPVSPQQKSVLVSPPAVSAGGVPPMPVIcqmVPLPANNPVVTTVVPSTPP--SQPPAVCPPVvfmgtQVPKGAVMFV 309
Cdd:PRK14951 369 AAEAAAPAEKKTPARPEAAAPAAAPVAQAAA---APAPAAAPAAAASAPAAPPaaAPPAPVAAPA-----AAAPAAAPAA 440

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*....
gi 3523146   310 VPQPVVQSSKPPVVSPNGTRLSP--IAPAPGFSPSAAKVTPQIDSSRIR 356
Cdd:PRK14951 441 APAAVALAPAPPAQAAPETVAIPvrVAPEPAVASAAPAPAAAPAAARLT 489

Zinc finger, C2H2 type; The C2H2 zinc finger is the classical zinc finger domain. The two conserved cysteines and histidines co-ordinate a zinc ion. The following pattern describes the zinc finger. #-X-C-X(1-5)-C-X3-#-X5-#-X2-H-X(3-6)-[H/C] Where X can be any amino acid, and numbers in brackets indicate the number of residues. The positions marked # are those that are important for the stable fold of the zinc finger. The final position can be either his or cys. The C2H2 zinc finger is composed of two short beta strands followed by an alpha helix. The amino terminal part of the helix binds the major groove in DNA binding zinc fingers. The accepted consensus binding sequence for Sp1 is usually defined by the asymmetric hexanucleotide core GGGCGG but this sequence does not include, among others, the GAG (=CTC) repeat that constitutes a high-affinity site for Sp1 binding to the wt1 promoter.

                          10        20
                  ....*....|....*....|....*
gi 3523146    358 HICSHpgCGKTYFKSSHLKAHTRTH 382
Cdd:pfam00096   1 YKCPD--CGKSFSRKSNLKRHLRTH 23

C2H2-type zinc ribbon; This family carries three zinc-fingers in tandem.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 3523146    360 CSHPGCGKTYFKSSHLKAHTRTHTGE------KPFSCSWKGCERRFARSDELSRHRRTHTGEKK-FACPMCDRRFMRSDH 432
Cdd:pfam15909   2 CSSPGCCLSFPSVRDLAQHLRTHCPPtqslegKLFRCSALSCTETFPSMQELVAHSKLHYKPNRyFKCENCLLRFRTHRS 81

                  ....
gi 3523146    433 LTKH 436
Cdd:pfam15909  82 LFKH 85