|
Name |
Accession |
Description |
Interval |
E-value |
| PTZ00176 super family |
cl31413 |
erythrocyte membrane protein 1 (PfEMP1); Provisional |
562-1323 |
4.89e-68 |
|
erythrocyte membrane protein 1 (PfEMP1); Provisional
The actual alignment was detected with superfamily member PTZ00176:
Pssm-ID: 140204 [Multi-domain] Cd Length: 1317 Bit Score: 254.96 E-value: 4.89e-68
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 562 KRKAHAQLEERGSRSALKGDASQGQYERKGKADDFKTKLCTITQD-HSNARSN---SKHPCDGKDgrNTRFQVGTGWKHD 637
Cdd:PTZ00176 29 KRENESKYTDKLKGSLWEARFSDGLSSSFGDVRSGYYDSCSLDHKfHTNINNGyppARNPCDGRN--QNRFDENGESYCN 106
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 638 NavSKIHKN-------VYMPPRRQHFCTSNLEYLINgkyeailkiQKGKINHSFLGDVLLAAKYQAEDTMKDYkPKGDQE 710
Cdd:PTZ00176 107 S--DKIRGNennsnagACAPFRRQNMCDKNLEYLIN---------KNTENTHDLLGNVLVTAKYEGESIVNNH-PDKDKS 174
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 711 GKCRAIRYSFADLGDIIKGTDLWKGNSGEQkTQRNLQRIFGKIKEELPGD-IQKKYTGTK---HLKLRADWWEANRAKVW 786
Cdd:PTZ00176 175 SVCTALARSFADIGDIVRGKDMFKRNKHDN-VENGLREVFKKIYEGLKNNgAREHYKEVKngnYIKLREDWWTANRDQVW 253
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 787 EAMKCHI----------GDLNDTSVHPSSSGNCGYSDHTPLDDYIPQRLRWMTEWAEWYCKEQSRLYGELVEKCAGCKGK 856
Cdd:PTZ00176 254 KAMTCVApenayfrkteADGIGISSLILPYSKCGRDTDPPVVDYIPQRLRWMSEWSEYFCNVLNKEIDEMNNQCKDCEMS 333
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 857 QKC---TEGDvDCGKCKPACENYKKFIKKWQPQWDKIRAKYKKIYEHARVDIAANGGLNTSTAIKDN-------EDKPVI 926
Cdd:PTZ00176 334 RRCnndTEGE-KCKKCKEQCQIFKELVSKWKNQFDKQSMKYMELYNKASTNITKQNSSAPERGYRRNhrrrgydDDTNVQ 412
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 927 EFLFELYKENGGKIGNPavaratvnGISTDDTTptvystaagyihqemgthmQCKEQNV---FCPSGGSKYAFKEPPDGY 1003
Cdd:PTZ00176 413 LFLKKVIENNECKVESL--------GKYLDKTS-------------------HCGNYNFnydNTPGSNRSNAFEITPEKF 465
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 1004 DVACKCD--ENKQKPPETPKENACEivkpllkdkgETDEIVQCNRKYKygKGNYPGWDcNSQI---HRTHNGACMPPRRQ 1078
Cdd:PTZ00176 466 KKACKCKipNPLEKCPNEENKNVCT----------RFDKVYSCTSLSF--KNDLSEWN-NSGVknkENDNNGVLVPPRRR 532
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 1079 KLCvINLqyFTGETsdglrkafiecaavetfflwHKYKEDNNGGEDLQN-QLKSGKI--------PEEFKRQMFYTFGDY 1149
Cdd:PTZ00176 533 NLC-INL--FSKKD--------------------YKMKDENDFKEDLLNaAFSQGKLlgkkysnySNEAYEAMKFSYADY 589
|
650 660 670 680 690 700 710 720
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 1150 RDFLFGTDISKNHgkgSKLANKIDSLFPPNNKKPGTLSRQKWWNENGPKIWNAMLCAlsYNTNEKtfkdkvHSQLTTTYg 1229
Cdd:PTZ00176 590 SDIVKGTDMMNDL---KKLNKELNTLLKETEKGDISVDRKTWWDDNKNVVWNAMLCG--YKTENE------NQQLNSSW- 657
|
730 740 750 760 770 780 790 800
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 1230 yskikfsdksTTLEEFAKRPQFLRWFTEWGEHFCKEHTKELAKLVTACPHDTCTNEGKKKV------CSDACANYKTFIK 1303
Cdd:PTZ00176 658 ----------CNVPDDDNIDQFLRWLTEWAQQYCKEKLIKAHIINTKCKDIVEGRKHKSMVditdveCKRLFIDYEEWFR 727
|
810 820
....*....|....*....|
gi 1035522805 1304 QWREEYKKQSKKYTEDKTAK 1323
Cdd:PTZ00176 728 YRYNQWKGLSEKYIKIKKSK 747
|
|
| PFEMP |
pfam03011 |
PFEMP1 DBL domain; PfEMP1 (Plasmodium falciparum erythrocyte membrane protein) has been ... |
2864-3010 |
6.85e-46 |
|
PFEMP1 DBL domain; PfEMP1 (Plasmodium falciparum erythrocyte membrane protein) has been identified as the rosetting ligand of the malaria parasite P. falciparum. Rosetting is the adhesion of infected erythrocytes with uninfected erythrocytes in the vasculature of the infected organ, and is associated with severe malaria. PfEMP1 interacts with Complement Receptor One on uninfected erythrocytes to form rosettes. The extreme variation within these proteins and the grouping of var genes implies that var gene recombination preferentially occurs within var gene groups. These groups reflect a functional diversification that has evolved to cope with the varying conditions of transmission and host immune response met by the parasite. A recombination hotspot was uncovered between Duffy-binding-like (DBL) subdomains. Solution of the crystal structure of the N-terminal and first DBL region of PfEMP1 from the VarO variant of the PfEMP1 protein is found to be directly implicated in rosetting as the heparin-binding site.
:
Pssm-ID: 281064 Cd Length: 154 Bit Score: 163.44 E-value: 6.85e-46
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 2864 LVTHWVHNFLEDYNRIKKKLKPCTKNDLESTCQNKCQNKCKCVGEWIKLKQQEWEEIKKRFLNQYK------MDSDEYYP 2937
Cdd:pfam03011 1 LFKRWVEYFLEDSIKWRKKLKSCINNGKGKCCKKECKNDCECFKKWVEKKKEEWKKIKEHFLKQYKlkgltgKTLDEYLD 80
|
90 100 110 120 130 140 150
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1035522805 2938 VRSVLETFLVQIGAANANNDVKKLIKLSEFYKSCGCSAKTNSE-NNKNEDAIDCMLDKLGEKAEKCAENHAQNS 3010
Cdd:pfam03011 81 LKSFLETFLFYIDIKEAYGDVKELKKLEEILDEEGCSAGAESAkNNKNEDAIDKLLDKEEKKANNCKEKHKEND 154
|
|
| Duffy_binding |
pfam05424 |
Duffy binding domain; This domain is found in Plasmodium Duffy binding proteins. Plasmodium ... |
2331-2507 |
1.65e-41 |
|
Duffy binding domain; This domain is found in Plasmodium Duffy binding proteins. Plasmodium vivax and Plasmodium knowlesi merozoites invade human erythrocytes that express Duffy blood group surface determinants. The Duffy receptor family is localized in micronemes, an organelle found in all organizms of the phylum Apicomplexa. This family is closely associated on PfEMP1 proteins with PFEMP, pfam03011.
:
Pssm-ID: 428465 [Multi-domain] Cd Length: 178 Bit Score: 151.66 E-value: 1.65e-41
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 2331 ICVPPRRRKLYLHKME----GVDTTTESLRKWFIETAAIETFFLWDRYKKEWEHRnkktqdglpgvgvgngddennpqkk 2406
Cdd:pfam05424 1 ACAPPRRLHLCVKNLEkinsGNTTTTHDLLKAVILAAKYEGFSLWHKYKKKNTKY------------------------- 55
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 2407 lqesGTIPIDFLRQMFYTLGDYRDILFGKNhIVIGKtpsvsakdemaKREEEIKNAIDNYFSKSGSTQT-----QPNSDK 2481
Cdd:pfam05424 56 ----GDIPSEFCRQMAYSFADIGDIIRGKD-LYLGN-----------NKKKKLEENLKKIFKKIYEKLTskgkdDGNYYQ 119
|
170 180
....*....|....*....|....*.
gi 1035522805 2482 DPKAWWNNNAKHIWKGMICALTYEDN 2507
Cdd:pfam05424 120 LREDWWEANREDIWKAMTCALTYGAK 145
|
|
| Duffy_binding |
pfam05424 |
Duffy binding domain; This domain is found in Plasmodium Duffy binding proteins. Plasmodium ... |
1451-1631 |
5.84e-39 |
|
Duffy binding domain; This domain is found in Plasmodium Duffy binding proteins. Plasmodium vivax and Plasmodium knowlesi merozoites invade human erythrocytes that express Duffy blood group surface determinants. The Duffy receptor family is localized in micronemes, an organelle found in all organizms of the phylum Apicomplexa. This family is closely associated on PfEMP1 proteins with PFEMP, pfam03011.
:
Pssm-ID: 428465 [Multi-domain] Cd Length: 178 Bit Score: 144.34 E-value: 5.84e-39
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 1451 ACMPPRRQKLCVINLKTFEPK---TSVELRNAFIKCAAIETHFLWKYYKEKNNVKndkilesGYIPEDFKRMMYYTFGDY 1527
Cdd:pfam05424 1 ACAPPRRLHLCVKNLEKINSGnttTTHDLLKAVILAAKYEGFSLWHKYKKKNTKY-------GDIPSEFCRQMAYSFADI 73
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 1528 RDLCLDKDI--GNDVSD-VENYIKGV-------LTDSTKNGGT-EITPDNWWKKIEKEVWDGMLCALSYNSKerSFKedv 1596
Cdd:pfam05424 74 GDIIRGKDLylGNNKKKkLEENLKKIfkkiyekLTSKGKDDGNyYQLREDWWEANREDIWKAMTCALTYGAK--YFR--- 148
|
170 180 190
....*....|....*....|....*....|....*
gi 1035522805 1597 rtqltTKYPYSTVKFSGGNNSTTLEEFAKRPQFLR 1631
Cdd:pfam05424 149 -----KTCGDGISPSTAGCNDDVPTYFDYVPQFLR 178
|
|
| Duffy_binding |
pfam05424 |
Duffy binding domain; This domain is found in Plasmodium Duffy binding proteins. Plasmodium ... |
1829-2003 |
1.17e-37 |
|
Duffy binding domain; This domain is found in Plasmodium Duffy binding proteins. Plasmodium vivax and Plasmodium knowlesi merozoites invade human erythrocytes that express Duffy blood group surface determinants. The Duffy receptor family is localized in micronemes, an organelle found in all organizms of the phylum Apicomplexa. This family is closely associated on PfEMP1 proteins with PFEMP, pfam03011.
:
Pssm-ID: 428465 [Multi-domain] Cd Length: 178 Bit Score: 140.87 E-value: 1.17e-37
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 1829 VCMPPRRQKLCLYFLTK--SNNLNSEDDIRNNFITCAAIETHFAWDRYKNKNKgaddqlKSGTIPDEFKRQMFYTFSDYR 1906
Cdd:pfam05424 1 ACAPPRRLHLCVKNLEKinSGNTTTTHDLLKAVILAAKYEGFSLWHKYKKKNT------KYGDIPSEFCRQMAYSFADIG 74
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 1907 NIFFGTDISSC--------QNIKNASENIKIILNKEKDEEKNEKQMINKWTNSYGPEIWEAMLCALvKIGAKKDDFTENY 1978
Cdd:pfam05424 75 DIIRGKDLYLGnnkkkkleENLKKIFKKIYEKLTSKGKDDGNYYQLREDWWEANREDIWKAMTCAL-TYGAKYFRKTCGD 153
|
170 180
....*....|....*....|....*
gi 1035522805 1979 GYNNVKFSDKSTTLEKFAQTPQFLR 2003
Cdd:pfam05424 154 GISPSTAGCNDDVPTYFDYVPQFLR 178
|
|
| Duffy_binding |
pfam05424 |
Duffy binding domain; This domain is found in Plasmodium Duffy binding proteins. Plasmodium ... |
1-111 |
2.44e-32 |
|
Duffy binding domain; This domain is found in Plasmodium Duffy binding proteins. Plasmodium vivax and Plasmodium knowlesi merozoites invade human erythrocytes that express Duffy blood group surface determinants. The Duffy receptor family is localized in micronemes, an organelle found in all organizms of the phylum Apicomplexa. This family is closely associated on PfEMP1 proteins with PFEMP, pfam03011.
:
Pssm-ID: 428465 [Multi-domain] Cd Length: 178 Bit Score: 125.46 E-value: 2.44e-32
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 1 DIVRGKDLYLGKNEsekrtKEKLQGNLVNIFKKFKEKY--GDLKDVPIDDIREYWWALNRKDVWKALTCNAPYEAQYFIK 78
Cdd:pfam05424 75 DIIRGKDLYLGNNK-----KKKLEENLKKIFKKIYEKLtsKGKDDGNYYQLREDWWEANREDIWKAMTCALTYGAKYFRK 149
|
90 100 110
....*....|....*....|....*....|...
gi 1035522805 79 SSRDDLTFSNEHCghykNGDPLTNLDYVPQFLR 111
Cdd:pfam05424 150 TCGDGISPSTAGC----NDDVPTYFDYVPQFLR 178
|
|
| CIDR1_gamma |
pfam18562 |
Cysteine-Rich Interdomain Region 1 gamma; Rosetting is the capacity of infected RBCs to bind ... |
2794-2844 |
2.36e-18 |
|
Cysteine-Rich Interdomain Region 1 gamma; Rosetting is the capacity of infected RBCs to bind uninfected RBCs, which is consistently associated with severe malaria in African children. The rosette-forming PfEMP1 adhesins, namely IT4/R29, Palo Alto 89F5 VarO, 3D7/PF13_0003 and IT4/var60, belong to a specific sub-group called groupA/UpsA var genes and all four present a specific Duffy Binding-Like and and Cysteine-Rich Interdomain Region (DBL1alpha1-CIDR1gamma) double domain Head region found at the extracellular region of PfEMP1. This entry represents the CIDR1gamma domain which increases the binding affinity to VarO (Palo Alto VarO parasites).
:
Pssm-ID: 408346 Cd Length: 52 Bit Score: 80.78 E-value: 2.36e-18
10 20 30 40 50
....*....|....*....|....*....|....*....|....*....|..
gi 1035522805 2794 TEKLDMRVIDDSATGF-GDLQDCTDANIFKSIRKDVWKCGKVCGYNVCKPEN 2844
Cdd:pfam18562 1 TTTIDMLVSDNRGIKFeNDLKECKEAGIFKGIRKDQWKCGKVCGYDVCKLKN 52
|
|
| PFEMP super family |
cl03834 |
PFEMP1 DBL domain; PfEMP1 (Plasmodium falciparum erythrocyte membrane protein) has been ... |
383-526 |
3.65e-14 |
|
PFEMP1 DBL domain; PfEMP1 (Plasmodium falciparum erythrocyte membrane protein) has been identified as the rosetting ligand of the malaria parasite P. falciparum. Rosetting is the adhesion of infected erythrocytes with uninfected erythrocytes in the vasculature of the infected organ, and is associated with severe malaria. PfEMP1 interacts with Complement Receptor One on uninfected erythrocytes to form rosettes. The extreme variation within these proteins and the grouping of var genes implies that var gene recombination preferentially occurs within var gene groups. These groups reflect a functional diversification that has evolved to cope with the varying conditions of transmission and host immune response met by the parasite. A recombination hotspot was uncovered between Duffy-binding-like (DBL) subdomains. Solution of the crystal structure of the N-terminal and first DBL region of PfEMP1 from the VarO variant of the PfEMP1 protein is found to be directly implicated in rosetting as the heparin-binding site.
The actual alignment was detected with superfamily member pfam03011:
Pssm-ID: 281064 Cd Length: 154 Bit Score: 72.53 E-value: 3.65e-14
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 383 LFHSWVRNLLIDTINWETDLRNCLNNTGITDCNDGCNNNCTCFYNWVKQKEKEWTNMMDLFTKEQKMPKKYYLNINDLFD 462
Cdd:pfam03011 1 LFKRWVEYFLEDSIKWRKKLKSCINNGKGKCCKKECKNDCECFKKWVEKKKEEWKKIKEHFLKQYKLKGLTGKTLDEYLD 80
|
90 100 110 120 130 140 150
....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 1035522805 463 SFFF---EVMDKLNQDETKWNKLKENLKKKIESSKKNAGTK-----DSEAAIKVLLDHLKEIATICKDNNTN 526
Cdd:pfam03011 81 LKSFletFLFYIDIKEAYGDVKELKKLEEILDEEGCSAGAEsaknnKNEDAIDKLLDKEEKKANNCKEKHKE 152
|
|
| |
|
Name |
Accession |
Description |
Interval |
E-value |
| PTZ00176 |
PTZ00176 |
erythrocyte membrane protein 1 (PfEMP1); Provisional |
562-1323 |
4.89e-68 |
|
erythrocyte membrane protein 1 (PfEMP1); Provisional
Pssm-ID: 140204 [Multi-domain] Cd Length: 1317 Bit Score: 254.96 E-value: 4.89e-68
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 562 KRKAHAQLEERGSRSALKGDASQGQYERKGKADDFKTKLCTITQD-HSNARSN---SKHPCDGKDgrNTRFQVGTGWKHD 637
Cdd:PTZ00176 29 KRENESKYTDKLKGSLWEARFSDGLSSSFGDVRSGYYDSCSLDHKfHTNINNGyppARNPCDGRN--QNRFDENGESYCN 106
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 638 NavSKIHKN-------VYMPPRRQHFCTSNLEYLINgkyeailkiQKGKINHSFLGDVLLAAKYQAEDTMKDYkPKGDQE 710
Cdd:PTZ00176 107 S--DKIRGNennsnagACAPFRRQNMCDKNLEYLIN---------KNTENTHDLLGNVLVTAKYEGESIVNNH-PDKDKS 174
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 711 GKCRAIRYSFADLGDIIKGTDLWKGNSGEQkTQRNLQRIFGKIKEELPGD-IQKKYTGTK---HLKLRADWWEANRAKVW 786
Cdd:PTZ00176 175 SVCTALARSFADIGDIVRGKDMFKRNKHDN-VENGLREVFKKIYEGLKNNgAREHYKEVKngnYIKLREDWWTANRDQVW 253
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 787 EAMKCHI----------GDLNDTSVHPSSSGNCGYSDHTPLDDYIPQRLRWMTEWAEWYCKEQSRLYGELVEKCAGCKGK 856
Cdd:PTZ00176 254 KAMTCVApenayfrkteADGIGISSLILPYSKCGRDTDPPVVDYIPQRLRWMSEWSEYFCNVLNKEIDEMNNQCKDCEMS 333
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 857 QKC---TEGDvDCGKCKPACENYKKFIKKWQPQWDKIRAKYKKIYEHARVDIAANGGLNTSTAIKDN-------EDKPVI 926
Cdd:PTZ00176 334 RRCnndTEGE-KCKKCKEQCQIFKELVSKWKNQFDKQSMKYMELYNKASTNITKQNSSAPERGYRRNhrrrgydDDTNVQ 412
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 927 EFLFELYKENGGKIGNPavaratvnGISTDDTTptvystaagyihqemgthmQCKEQNV---FCPSGGSKYAFKEPPDGY 1003
Cdd:PTZ00176 413 LFLKKVIENNECKVESL--------GKYLDKTS-------------------HCGNYNFnydNTPGSNRSNAFEITPEKF 465
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 1004 DVACKCD--ENKQKPPETPKENACEivkpllkdkgETDEIVQCNRKYKygKGNYPGWDcNSQI---HRTHNGACMPPRRQ 1078
Cdd:PTZ00176 466 KKACKCKipNPLEKCPNEENKNVCT----------RFDKVYSCTSLSF--KNDLSEWN-NSGVknkENDNNGVLVPPRRR 532
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 1079 KLCvINLqyFTGETsdglrkafiecaavetfflwHKYKEDNNGGEDLQN-QLKSGKI--------PEEFKRQMFYTFGDY 1149
Cdd:PTZ00176 533 NLC-INL--FSKKD--------------------YKMKDENDFKEDLLNaAFSQGKLlgkkysnySNEAYEAMKFSYADY 589
|
650 660 670 680 690 700 710 720
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 1150 RDFLFGTDISKNHgkgSKLANKIDSLFPPNNKKPGTLSRQKWWNENGPKIWNAMLCAlsYNTNEKtfkdkvHSQLTTTYg 1229
Cdd:PTZ00176 590 SDIVKGTDMMNDL---KKLNKELNTLLKETEKGDISVDRKTWWDDNKNVVWNAMLCG--YKTENE------NQQLNSSW- 657
|
730 740 750 760 770 780 790 800
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 1230 yskikfsdksTTLEEFAKRPQFLRWFTEWGEHFCKEHTKELAKLVTACPHDTCTNEGKKKV------CSDACANYKTFIK 1303
Cdd:PTZ00176 658 ----------CNVPDDDNIDQFLRWLTEWAQQYCKEKLIKAHIINTKCKDIVEGRKHKSMVditdveCKRLFIDYEEWFR 727
|
810 820
....*....|....*....|
gi 1035522805 1304 QWREEYKKQSKKYTEDKTAK 1323
Cdd:PTZ00176 728 YRYNQWKGLSEKYIKIKKSK 747
|
|
| Duffy_binding |
pfam05424 |
Duffy binding domain; This domain is found in Plasmodium Duffy binding proteins. Plasmodium ... |
1071-1253 |
7.39e-50 |
|
Duffy binding domain; This domain is found in Plasmodium Duffy binding proteins. Plasmodium vivax and Plasmodium knowlesi merozoites invade human erythrocytes that express Duffy blood group surface determinants. The Duffy receptor family is localized in micronemes, an organelle found in all organizms of the phylum Apicomplexa. This family is closely associated on PfEMP1 proteins with PFEMP, pfam03011.
Pssm-ID: 428465 [Multi-domain] Cd Length: 178 Bit Score: 175.54 E-value: 7.39e-50
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 1071 ACMPPRRQKLCVINLQYFTGE---TSDGLRKAFIECAAVETFFLWHKYKEDNNggedlqnqlKSGKIPEEFKRQMFYTFG 1147
Cdd:pfam05424 1 ACAPPRRLHLCVKNLEKINSGnttTTHDLLKAVILAAKYEGFSLWHKYKKKNT---------KYGDIPSEFCRQMAYSFA 71
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 1148 DYRDFLFGTDISKNHGKGSKLANKIDSLFPPNNKKPGTLS---------RQKWWNENGPKIWNAMLCALSYntNEKTFKD 1218
Cdd:pfam05424 72 DIGDIIRGKDLYLGNNKKKKLEENLKKIFKKIYEKLTSKGkddgnyyqlREDWWEANREDIWKAMTCALTY--GAKYFRK 149
|
170 180 190
....*....|....*....|....*....|....*
gi 1035522805 1219 KVHSQLTTTygySKIKFSDKSTTleeFAKRPQFLR 1253
Cdd:pfam05424 150 TCGDGISPS---TAGCNDDVPTY---FDYVPQFLR 178
|
|
| PFEMP |
pfam03011 |
PFEMP1 DBL domain; PfEMP1 (Plasmodium falciparum erythrocyte membrane protein) has been ... |
2864-3010 |
6.85e-46 |
|
PFEMP1 DBL domain; PfEMP1 (Plasmodium falciparum erythrocyte membrane protein) has been identified as the rosetting ligand of the malaria parasite P. falciparum. Rosetting is the adhesion of infected erythrocytes with uninfected erythrocytes in the vasculature of the infected organ, and is associated with severe malaria. PfEMP1 interacts with Complement Receptor One on uninfected erythrocytes to form rosettes. The extreme variation within these proteins and the grouping of var genes implies that var gene recombination preferentially occurs within var gene groups. These groups reflect a functional diversification that has evolved to cope with the varying conditions of transmission and host immune response met by the parasite. A recombination hotspot was uncovered between Duffy-binding-like (DBL) subdomains. Solution of the crystal structure of the N-terminal and first DBL region of PfEMP1 from the VarO variant of the PfEMP1 protein is found to be directly implicated in rosetting as the heparin-binding site.
Pssm-ID: 281064 Cd Length: 154 Bit Score: 163.44 E-value: 6.85e-46
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 2864 LVTHWVHNFLEDYNRIKKKLKPCTKNDLESTCQNKCQNKCKCVGEWIKLKQQEWEEIKKRFLNQYK------MDSDEYYP 2937
Cdd:pfam03011 1 LFKRWVEYFLEDSIKWRKKLKSCINNGKGKCCKKECKNDCECFKKWVEKKKEEWKKIKEHFLKQYKlkgltgKTLDEYLD 80
|
90 100 110 120 130 140 150
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1035522805 2938 VRSVLETFLVQIGAANANNDVKKLIKLSEFYKSCGCSAKTNSE-NNKNEDAIDCMLDKLGEKAEKCAENHAQNS 3010
Cdd:pfam03011 81 LKSFLETFLFYIDIKEAYGDVKELKKLEEILDEEGCSAGAESAkNNKNEDAIDKLLDKEEKKANNCKEKHKEND 154
|
|
| Duffy_binding |
pfam05424 |
Duffy binding domain; This domain is found in Plasmodium Duffy binding proteins. Plasmodium ... |
2331-2507 |
1.65e-41 |
|
Duffy binding domain; This domain is found in Plasmodium Duffy binding proteins. Plasmodium vivax and Plasmodium knowlesi merozoites invade human erythrocytes that express Duffy blood group surface determinants. The Duffy receptor family is localized in micronemes, an organelle found in all organizms of the phylum Apicomplexa. This family is closely associated on PfEMP1 proteins with PFEMP, pfam03011.
Pssm-ID: 428465 [Multi-domain] Cd Length: 178 Bit Score: 151.66 E-value: 1.65e-41
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 2331 ICVPPRRRKLYLHKME----GVDTTTESLRKWFIETAAIETFFLWDRYKKEWEHRnkktqdglpgvgvgngddennpqkk 2406
Cdd:pfam05424 1 ACAPPRRLHLCVKNLEkinsGNTTTTHDLLKAVILAAKYEGFSLWHKYKKKNTKY------------------------- 55
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 2407 lqesGTIPIDFLRQMFYTLGDYRDILFGKNhIVIGKtpsvsakdemaKREEEIKNAIDNYFSKSGSTQT-----QPNSDK 2481
Cdd:pfam05424 56 ----GDIPSEFCRQMAYSFADIGDIIRGKD-LYLGN-----------NKKKKLEENLKKIFKKIYEKLTskgkdDGNYYQ 119
|
170 180
....*....|....*....|....*.
gi 1035522805 2482 DPKAWWNNNAKHIWKGMICALTYEDN 2507
Cdd:pfam05424 120 LREDWWEANREDIWKAMTCALTYGAK 145
|
|
| Duffy_binding |
pfam05424 |
Duffy binding domain; This domain is found in Plasmodium Duffy binding proteins. Plasmodium ... |
1451-1631 |
5.84e-39 |
|
Duffy binding domain; This domain is found in Plasmodium Duffy binding proteins. Plasmodium vivax and Plasmodium knowlesi merozoites invade human erythrocytes that express Duffy blood group surface determinants. The Duffy receptor family is localized in micronemes, an organelle found in all organizms of the phylum Apicomplexa. This family is closely associated on PfEMP1 proteins with PFEMP, pfam03011.
Pssm-ID: 428465 [Multi-domain] Cd Length: 178 Bit Score: 144.34 E-value: 5.84e-39
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 1451 ACMPPRRQKLCVINLKTFEPK---TSVELRNAFIKCAAIETHFLWKYYKEKNNVKndkilesGYIPEDFKRMMYYTFGDY 1527
Cdd:pfam05424 1 ACAPPRRLHLCVKNLEKINSGnttTTHDLLKAVILAAKYEGFSLWHKYKKKNTKY-------GDIPSEFCRQMAYSFADI 73
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 1528 RDLCLDKDI--GNDVSD-VENYIKGV-------LTDSTKNGGT-EITPDNWWKKIEKEVWDGMLCALSYNSKerSFKedv 1596
Cdd:pfam05424 74 GDIIRGKDLylGNNKKKkLEENLKKIfkkiyekLTSKGKDDGNyYQLREDWWEANREDIWKAMTCALTYGAK--YFR--- 148
|
170 180 190
....*....|....*....|....*....|....*
gi 1035522805 1597 rtqltTKYPYSTVKFSGGNNSTTLEEFAKRPQFLR 1631
Cdd:pfam05424 149 -----KTCGDGISPSTAGCNDDVPTYFDYVPQFLR 178
|
|
| Duffy_binding |
pfam05424 |
Duffy binding domain; This domain is found in Plasmodium Duffy binding proteins. Plasmodium ... |
1829-2003 |
1.17e-37 |
|
Duffy binding domain; This domain is found in Plasmodium Duffy binding proteins. Plasmodium vivax and Plasmodium knowlesi merozoites invade human erythrocytes that express Duffy blood group surface determinants. The Duffy receptor family is localized in micronemes, an organelle found in all organizms of the phylum Apicomplexa. This family is closely associated on PfEMP1 proteins with PFEMP, pfam03011.
Pssm-ID: 428465 [Multi-domain] Cd Length: 178 Bit Score: 140.87 E-value: 1.17e-37
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 1829 VCMPPRRQKLCLYFLTK--SNNLNSEDDIRNNFITCAAIETHFAWDRYKNKNKgaddqlKSGTIPDEFKRQMFYTFSDYR 1906
Cdd:pfam05424 1 ACAPPRRLHLCVKNLEKinSGNTTTTHDLLKAVILAAKYEGFSLWHKYKKKNT------KYGDIPSEFCRQMAYSFADIG 74
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 1907 NIFFGTDISSC--------QNIKNASENIKIILNKEKDEEKNEKQMINKWTNSYGPEIWEAMLCALvKIGAKKDDFTENY 1978
Cdd:pfam05424 75 DIIRGKDLYLGnnkkkkleENLKKIFKKIYEKLTSKGKDDGNYYQLREDWWEANREDIWKAMTCAL-TYGAKYFRKTCGD 153
|
170 180
....*....|....*....|....*
gi 1035522805 1979 GYNNVKFSDKSTTLEKFAQTPQFLR 2003
Cdd:pfam05424 154 GISPSTAGCNDDVPTYFDYVPQFLR 178
|
|
| Duffy_binding |
pfam05424 |
Duffy binding domain; This domain is found in Plasmodium Duffy binding proteins. Plasmodium ... |
1-111 |
2.44e-32 |
|
Duffy binding domain; This domain is found in Plasmodium Duffy binding proteins. Plasmodium vivax and Plasmodium knowlesi merozoites invade human erythrocytes that express Duffy blood group surface determinants. The Duffy receptor family is localized in micronemes, an organelle found in all organizms of the phylum Apicomplexa. This family is closely associated on PfEMP1 proteins with PFEMP, pfam03011.
Pssm-ID: 428465 [Multi-domain] Cd Length: 178 Bit Score: 125.46 E-value: 2.44e-32
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 1 DIVRGKDLYLGKNEsekrtKEKLQGNLVNIFKKFKEKY--GDLKDVPIDDIREYWWALNRKDVWKALTCNAPYEAQYFIK 78
Cdd:pfam05424 75 DIIRGKDLYLGNNK-----KKKLEENLKKIFKKIYEKLtsKGKDDGNYYQLREDWWEANREDIWKAMTCALTYGAKYFRK 149
|
90 100 110
....*....|....*....|....*....|...
gi 1035522805 79 SSRDDLTFSNEHCghykNGDPLTNLDYVPQFLR 111
Cdd:pfam05424 150 TCGDGISPSTAGC----NDDVPTYFDYVPQFLR 178
|
|
| PTZ00176 |
PTZ00176 |
erythrocyte membrane protein 1 (PfEMP1); Provisional |
1-195 |
1.45e-23 |
|
erythrocyte membrane protein 1 (PfEMP1); Provisional
Pssm-ID: 140204 [Multi-domain] Cd Length: 1317 Bit Score: 110.51 E-value: 1.45e-23
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 1 DIVRGKDLYlgknesEKRTKEKLQGNLVNIFKKF---------KEKYGDLKDVPIDDIREYWWALNRKDVWKALTCNAPy 71
Cdd:PTZ00176 189 DIVRGKDMF------KRNKHDNVENGLREVFKKIyeglknngaREHYKEVKNGNYIKLREDWWTANRDQVWKAMTCVAP- 261
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 72 EAQYFIKSSRDDLTFSN-----EHCGHykNGDPLTnLDYVPQFLRWFEEWAEHFCLVRKYKLEKVKEACRgktggmycsh 146
Cdd:PTZ00176 262 ENAYFRKTEADGIGISSlilpySKCGR--DTDPPV-VDYIPQRLRWMSEWSEYFCNVLNKEIDEMNNQCK---------- 328
|
170 180 190 200
....*....|....*....|....*....|....*....|....*....
gi 1035522805 147 ngcDCEKTiGKIRHFVWDHKCNKCSIECGLYQNWMKDQKLEFEKQKEKY 195
Cdd:PTZ00176 329 ---DCEMS-RRCNNDTEGEKCKKCKEQCQIFKELVSKWKNQFDKQSMKY 373
|
|
| CIDR1_gamma |
pfam18562 |
Cysteine-Rich Interdomain Region 1 gamma; Rosetting is the capacity of infected RBCs to bind ... |
2794-2844 |
2.36e-18 |
|
Cysteine-Rich Interdomain Region 1 gamma; Rosetting is the capacity of infected RBCs to bind uninfected RBCs, which is consistently associated with severe malaria in African children. The rosette-forming PfEMP1 adhesins, namely IT4/R29, Palo Alto 89F5 VarO, 3D7/PF13_0003 and IT4/var60, belong to a specific sub-group called groupA/UpsA var genes and all four present a specific Duffy Binding-Like and and Cysteine-Rich Interdomain Region (DBL1alpha1-CIDR1gamma) double domain Head region found at the extracellular region of PfEMP1. This entry represents the CIDR1gamma domain which increases the binding affinity to VarO (Palo Alto VarO parasites).
Pssm-ID: 408346 Cd Length: 52 Bit Score: 80.78 E-value: 2.36e-18
10 20 30 40 50
....*....|....*....|....*....|....*....|....*....|..
gi 1035522805 2794 TEKLDMRVIDDSATGF-GDLQDCTDANIFKSIRKDVWKCGKVCGYNVCKPEN 2844
Cdd:pfam18562 1 TTTIDMLVSDNRGIKFeNDLKECKEAGIFKGIRKDQWKCGKVCGYDVCKLKN 52
|
|
| PFEMP |
pfam03011 |
PFEMP1 DBL domain; PfEMP1 (Plasmodium falciparum erythrocyte membrane protein) has been ... |
383-526 |
3.65e-14 |
|
PFEMP1 DBL domain; PfEMP1 (Plasmodium falciparum erythrocyte membrane protein) has been identified as the rosetting ligand of the malaria parasite P. falciparum. Rosetting is the adhesion of infected erythrocytes with uninfected erythrocytes in the vasculature of the infected organ, and is associated with severe malaria. PfEMP1 interacts with Complement Receptor One on uninfected erythrocytes to form rosettes. The extreme variation within these proteins and the grouping of var genes implies that var gene recombination preferentially occurs within var gene groups. These groups reflect a functional diversification that has evolved to cope with the varying conditions of transmission and host immune response met by the parasite. A recombination hotspot was uncovered between Duffy-binding-like (DBL) subdomains. Solution of the crystal structure of the N-terminal and first DBL region of PfEMP1 from the VarO variant of the PfEMP1 protein is found to be directly implicated in rosetting as the heparin-binding site.
Pssm-ID: 281064 Cd Length: 154 Bit Score: 72.53 E-value: 3.65e-14
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 383 LFHSWVRNLLIDTINWETDLRNCLNNTGITDCNDGCNNNCTCFYNWVKQKEKEWTNMMDLFTKEQKMPKKYYLNINDLFD 462
Cdd:pfam03011 1 LFKRWVEYFLEDSIKWRKKLKSCINNGKGKCCKKECKNDCECFKKWVEKKKEEWKKIKEHFLKQYKLKGLTGKTLDEYLD 80
|
90 100 110 120 130 140 150
....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 1035522805 463 SFFF---EVMDKLNQDETKWNKLKENLKKKIESSKKNAGTK-----DSEAAIKVLLDHLKEIATICKDNNTN 526
Cdd:pfam03011 81 LKSFletFLFYIDIKEAYGDVKELKKLEEILDEEGCSAGAEsaknnKNEDAIDKLLDKEEKKANNCKEKHKE 152
|
|
| PTZ00176 |
PTZ00176 |
erythrocyte membrane protein 1 (PfEMP1); Provisional |
2328-2680 |
1.73e-06 |
|
erythrocyte membrane protein 1 (PfEMP1); Provisional
Pssm-ID: 140204 [Multi-domain] Cd Length: 1317 Bit Score: 54.27 E-value: 1.73e-06
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 2328 DGAICVPPRRRKLYLHKMEGVDT----TTESLRKWFIETAAIEtfflwdrykkewehrnkktqdglpgvgvGNGDDENNP 2403
Cdd:PTZ00176 118 NAGACAPFRRQNMCDKNLEYLINknteNTHDLLGNVLVTAKYE----------------------------GESIVNNHP 169
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 2404 QKklqESGTIPIDFLRqmfyTLGDYRDILFGKNHIvigktpSVSAKDEMAKREEEIKNAIDNYFSKSGSTQTQP-----N 2478
Cdd:PTZ00176 170 DK---DKSSVCTALAR----SFADIGDIVRGKDMF------KRNKHDNVENGLREVFKKIYEGLKNNGAREHYKevkngN 236
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 2479 SDKDPKAWWNNNAKHIWKGMICAltyedngekgTPQvndslyNKFFgtqngnprlqpvtpgkppnpgtnTGTYKEKYDYE 2558
Cdd:PTZ00176 237 YIKLREDWWTANRDQVWKAMTCV----------APE------NAYF-----------------------RKTEADGIGIS 277
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 2559 NVTLkdeqsgggPKPTGGDSTlnNPKLTQFVlrPPYFRYLEEWGETFCKERKKRLDQIYRECKvdsddykcsgygeDCKN 2638
Cdd:PTZ00176 278 SLIL--------PYSKCGRDT--DPPVVDYI--PQRLRWMSEWSEYFCNVLNKEIDEMNNQCK-------------DCEM 332
|
330 340 350 360
....*....|....*....|....*....|....*....|..
gi 1035522805 2639 NLREKYDTvPSLECPDCAKHCSFYKKWIKKKRTEFNKQSNVY 2680
Cdd:PTZ00176 333 SRRCNNDT-EGEKCKKCKEQCQIFKELVSKWKNQFDKQSMKY 373
|
|
| |
|
Name |
Accession |
Description |
Interval |
E-value |
| PTZ00176 |
PTZ00176 |
erythrocyte membrane protein 1 (PfEMP1); Provisional |
562-1323 |
4.89e-68 |
|
erythrocyte membrane protein 1 (PfEMP1); Provisional
Pssm-ID: 140204 [Multi-domain] Cd Length: 1317 Bit Score: 254.96 E-value: 4.89e-68
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 562 KRKAHAQLEERGSRSALKGDASQGQYERKGKADDFKTKLCTITQD-HSNARSN---SKHPCDGKDgrNTRFQVGTGWKHD 637
Cdd:PTZ00176 29 KRENESKYTDKLKGSLWEARFSDGLSSSFGDVRSGYYDSCSLDHKfHTNINNGyppARNPCDGRN--QNRFDENGESYCN 106
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 638 NavSKIHKN-------VYMPPRRQHFCTSNLEYLINgkyeailkiQKGKINHSFLGDVLLAAKYQAEDTMKDYkPKGDQE 710
Cdd:PTZ00176 107 S--DKIRGNennsnagACAPFRRQNMCDKNLEYLIN---------KNTENTHDLLGNVLVTAKYEGESIVNNH-PDKDKS 174
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 711 GKCRAIRYSFADLGDIIKGTDLWKGNSGEQkTQRNLQRIFGKIKEELPGD-IQKKYTGTK---HLKLRADWWEANRAKVW 786
Cdd:PTZ00176 175 SVCTALARSFADIGDIVRGKDMFKRNKHDN-VENGLREVFKKIYEGLKNNgAREHYKEVKngnYIKLREDWWTANRDQVW 253
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 787 EAMKCHI----------GDLNDTSVHPSSSGNCGYSDHTPLDDYIPQRLRWMTEWAEWYCKEQSRLYGELVEKCAGCKGK 856
Cdd:PTZ00176 254 KAMTCVApenayfrkteADGIGISSLILPYSKCGRDTDPPVVDYIPQRLRWMSEWSEYFCNVLNKEIDEMNNQCKDCEMS 333
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 857 QKC---TEGDvDCGKCKPACENYKKFIKKWQPQWDKIRAKYKKIYEHARVDIAANGGLNTSTAIKDN-------EDKPVI 926
Cdd:PTZ00176 334 RRCnndTEGE-KCKKCKEQCQIFKELVSKWKNQFDKQSMKYMELYNKASTNITKQNSSAPERGYRRNhrrrgydDDTNVQ 412
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 927 EFLFELYKENGGKIGNPavaratvnGISTDDTTptvystaagyihqemgthmQCKEQNV---FCPSGGSKYAFKEPPDGY 1003
Cdd:PTZ00176 413 LFLKKVIENNECKVESL--------GKYLDKTS-------------------HCGNYNFnydNTPGSNRSNAFEITPEKF 465
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 1004 DVACKCD--ENKQKPPETPKENACEivkpllkdkgETDEIVQCNRKYKygKGNYPGWDcNSQI---HRTHNGACMPPRRQ 1078
Cdd:PTZ00176 466 KKACKCKipNPLEKCPNEENKNVCT----------RFDKVYSCTSLSF--KNDLSEWN-NSGVknkENDNNGVLVPPRRR 532
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 1079 KLCvINLqyFTGETsdglrkafiecaavetfflwHKYKEDNNGGEDLQN-QLKSGKI--------PEEFKRQMFYTFGDY 1149
Cdd:PTZ00176 533 NLC-INL--FSKKD--------------------YKMKDENDFKEDLLNaAFSQGKLlgkkysnySNEAYEAMKFSYADY 589
|
650 660 670 680 690 700 710 720
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 1150 RDFLFGTDISKNHgkgSKLANKIDSLFPPNNKKPGTLSRQKWWNENGPKIWNAMLCAlsYNTNEKtfkdkvHSQLTTTYg 1229
Cdd:PTZ00176 590 SDIVKGTDMMNDL---KKLNKELNTLLKETEKGDISVDRKTWWDDNKNVVWNAMLCG--YKTENE------NQQLNSSW- 657
|
730 740 750 760 770 780 790 800
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 1230 yskikfsdksTTLEEFAKRPQFLRWFTEWGEHFCKEHTKELAKLVTACPHDTCTNEGKKKV------CSDACANYKTFIK 1303
Cdd:PTZ00176 658 ----------CNVPDDDNIDQFLRWLTEWAQQYCKEKLIKAHIINTKCKDIVEGRKHKSMVditdveCKRLFIDYEEWFR 727
|
810 820
....*....|....*....|
gi 1035522805 1304 QWREEYKKQSKKYTEDKTAK 1323
Cdd:PTZ00176 728 YRYNQWKGLSEKYIKIKKSK 747
|
|
| Duffy_binding |
pfam05424 |
Duffy binding domain; This domain is found in Plasmodium Duffy binding proteins. Plasmodium ... |
1071-1253 |
7.39e-50 |
|
Duffy binding domain; This domain is found in Plasmodium Duffy binding proteins. Plasmodium vivax and Plasmodium knowlesi merozoites invade human erythrocytes that express Duffy blood group surface determinants. The Duffy receptor family is localized in micronemes, an organelle found in all organizms of the phylum Apicomplexa. This family is closely associated on PfEMP1 proteins with PFEMP, pfam03011.
Pssm-ID: 428465 [Multi-domain] Cd Length: 178 Bit Score: 175.54 E-value: 7.39e-50
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 1071 ACMPPRRQKLCVINLQYFTGE---TSDGLRKAFIECAAVETFFLWHKYKEDNNggedlqnqlKSGKIPEEFKRQMFYTFG 1147
Cdd:pfam05424 1 ACAPPRRLHLCVKNLEKINSGnttTTHDLLKAVILAAKYEGFSLWHKYKKKNT---------KYGDIPSEFCRQMAYSFA 71
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 1148 DYRDFLFGTDISKNHGKGSKLANKIDSLFPPNNKKPGTLS---------RQKWWNENGPKIWNAMLCALSYntNEKTFKD 1218
Cdd:pfam05424 72 DIGDIIRGKDLYLGNNKKKKLEENLKKIFKKIYEKLTSKGkddgnyyqlREDWWEANREDIWKAMTCALTY--GAKYFRK 149
|
170 180 190
....*....|....*....|....*....|....*
gi 1035522805 1219 KVHSQLTTTygySKIKFSDKSTTleeFAKRPQFLR 1253
Cdd:pfam05424 150 TCGDGISPS---TAGCNDDVPTY---FDYVPQFLR 178
|
|
| Duffy_binding |
pfam05424 |
Duffy binding domain; This domain is found in Plasmodium Duffy binding proteins. Plasmodium ... |
647-826 |
8.14e-50 |
|
Duffy binding domain; This domain is found in Plasmodium Duffy binding proteins. Plasmodium vivax and Plasmodium knowlesi merozoites invade human erythrocytes that express Duffy blood group surface determinants. The Duffy receptor family is localized in micronemes, an organelle found in all organizms of the phylum Apicomplexa. This family is closely associated on PfEMP1 proteins with PFEMP, pfam03011.
Pssm-ID: 428465 [Multi-domain] Cd Length: 178 Bit Score: 175.54 E-value: 8.14e-50
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 647 VYMPPRRQHFCTSNLEYLINGKYEAilkiqkgkiNHSFLGDVLLAAKYQAEDTMKDYKPKGDQEGK-----CRAIRYSFA 721
Cdd:pfam05424 1 ACAPPRRLHLCVKNLEKINSGNTTT---------THDLLKAVILAAKYEGFSLWHKYKKKNTKYGDipsefCRQMAYSFA 71
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 722 DLGDIIKGTDLWKGNSGEQKTQRNLQRIFGKIKEELPGDIQKKytgTKHLKLRADWWEANRAKVWEAMKCHIGDLND--- 798
Cdd:pfam05424 72 DIGDIIRGKDLYLGNNKKKKLEENLKKIFKKIYEKLTSKGKDD---GNYYQLREDWWEANREDIWKAMTCALTYGAKyfr 148
|
170 180 190
....*....|....*....|....*....|
gi 1035522805 799 --TSVHPSSSGNCGYSDHTPLDDYIPQRLR 826
Cdd:pfam05424 149 ktCGDGISPSTAGCNDDVPTYFDYVPQFLR 178
|
|
| PFEMP |
pfam03011 |
PFEMP1 DBL domain; PfEMP1 (Plasmodium falciparum erythrocyte membrane protein) has been ... |
2864-3010 |
6.85e-46 |
|
PFEMP1 DBL domain; PfEMP1 (Plasmodium falciparum erythrocyte membrane protein) has been identified as the rosetting ligand of the malaria parasite P. falciparum. Rosetting is the adhesion of infected erythrocytes with uninfected erythrocytes in the vasculature of the infected organ, and is associated with severe malaria. PfEMP1 interacts with Complement Receptor One on uninfected erythrocytes to form rosettes. The extreme variation within these proteins and the grouping of var genes implies that var gene recombination preferentially occurs within var gene groups. These groups reflect a functional diversification that has evolved to cope with the varying conditions of transmission and host immune response met by the parasite. A recombination hotspot was uncovered between Duffy-binding-like (DBL) subdomains. Solution of the crystal structure of the N-terminal and first DBL region of PfEMP1 from the VarO variant of the PfEMP1 protein is found to be directly implicated in rosetting as the heparin-binding site.
Pssm-ID: 281064 Cd Length: 154 Bit Score: 163.44 E-value: 6.85e-46
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 2864 LVTHWVHNFLEDYNRIKKKLKPCTKNDLESTCQNKCQNKCKCVGEWIKLKQQEWEEIKKRFLNQYK------MDSDEYYP 2937
Cdd:pfam03011 1 LFKRWVEYFLEDSIKWRKKLKSCINNGKGKCCKKECKNDCECFKKWVEKKKEEWKKIKEHFLKQYKlkgltgKTLDEYLD 80
|
90 100 110 120 130 140 150
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1035522805 2938 VRSVLETFLVQIGAANANNDVKKLIKLSEFYKSCGCSAKTNSE-NNKNEDAIDCMLDKLGEKAEKCAENHAQNS 3010
Cdd:pfam03011 81 LKSFLETFLFYIDIKEAYGDVKELKKLEEILDEEGCSAGAESAkNNKNEDAIDKLLDKEEKKANNCKEKHKEND 154
|
|
| Duffy_binding |
pfam05424 |
Duffy binding domain; This domain is found in Plasmodium Duffy binding proteins. Plasmodium ... |
2331-2507 |
1.65e-41 |
|
Duffy binding domain; This domain is found in Plasmodium Duffy binding proteins. Plasmodium vivax and Plasmodium knowlesi merozoites invade human erythrocytes that express Duffy blood group surface determinants. The Duffy receptor family is localized in micronemes, an organelle found in all organizms of the phylum Apicomplexa. This family is closely associated on PfEMP1 proteins with PFEMP, pfam03011.
Pssm-ID: 428465 [Multi-domain] Cd Length: 178 Bit Score: 151.66 E-value: 1.65e-41
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 2331 ICVPPRRRKLYLHKME----GVDTTTESLRKWFIETAAIETFFLWDRYKKEWEHRnkktqdglpgvgvgngddennpqkk 2406
Cdd:pfam05424 1 ACAPPRRLHLCVKNLEkinsGNTTTTHDLLKAVILAAKYEGFSLWHKYKKKNTKY------------------------- 55
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 2407 lqesGTIPIDFLRQMFYTLGDYRDILFGKNhIVIGKtpsvsakdemaKREEEIKNAIDNYFSKSGSTQT-----QPNSDK 2481
Cdd:pfam05424 56 ----GDIPSEFCRQMAYSFADIGDIIRGKD-LYLGN-----------NKKKKLEENLKKIFKKIYEKLTskgkdDGNYYQ 119
|
170 180
....*....|....*....|....*.
gi 1035522805 2482 DPKAWWNNNAKHIWKGMICALTYEDN 2507
Cdd:pfam05424 120 LREDWWEANREDIWKAMTCALTYGAK 145
|
|
| Duffy_binding |
pfam05424 |
Duffy binding domain; This domain is found in Plasmodium Duffy binding proteins. Plasmodium ... |
1451-1631 |
5.84e-39 |
|
Duffy binding domain; This domain is found in Plasmodium Duffy binding proteins. Plasmodium vivax and Plasmodium knowlesi merozoites invade human erythrocytes that express Duffy blood group surface determinants. The Duffy receptor family is localized in micronemes, an organelle found in all organizms of the phylum Apicomplexa. This family is closely associated on PfEMP1 proteins with PFEMP, pfam03011.
Pssm-ID: 428465 [Multi-domain] Cd Length: 178 Bit Score: 144.34 E-value: 5.84e-39
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 1451 ACMPPRRQKLCVINLKTFEPK---TSVELRNAFIKCAAIETHFLWKYYKEKNNVKndkilesGYIPEDFKRMMYYTFGDY 1527
Cdd:pfam05424 1 ACAPPRRLHLCVKNLEKINSGnttTTHDLLKAVILAAKYEGFSLWHKYKKKNTKY-------GDIPSEFCRQMAYSFADI 73
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 1528 RDLCLDKDI--GNDVSD-VENYIKGV-------LTDSTKNGGT-EITPDNWWKKIEKEVWDGMLCALSYNSKerSFKedv 1596
Cdd:pfam05424 74 GDIIRGKDLylGNNKKKkLEENLKKIfkkiyekLTSKGKDDGNyYQLREDWWEANREDIWKAMTCALTYGAK--YFR--- 148
|
170 180 190
....*....|....*....|....*....|....*
gi 1035522805 1597 rtqltTKYPYSTVKFSGGNNSTTLEEFAKRPQFLR 1631
Cdd:pfam05424 149 -----KTCGDGISPSTAGCNDDVPTYFDYVPQFLR 178
|
|
| Duffy_binding |
pfam05424 |
Duffy binding domain; This domain is found in Plasmodium Duffy binding proteins. Plasmodium ... |
1829-2003 |
1.17e-37 |
|
Duffy binding domain; This domain is found in Plasmodium Duffy binding proteins. Plasmodium vivax and Plasmodium knowlesi merozoites invade human erythrocytes that express Duffy blood group surface determinants. The Duffy receptor family is localized in micronemes, an organelle found in all organizms of the phylum Apicomplexa. This family is closely associated on PfEMP1 proteins with PFEMP, pfam03011.
Pssm-ID: 428465 [Multi-domain] Cd Length: 178 Bit Score: 140.87 E-value: 1.17e-37
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 1829 VCMPPRRQKLCLYFLTK--SNNLNSEDDIRNNFITCAAIETHFAWDRYKNKNKgaddqlKSGTIPDEFKRQMFYTFSDYR 1906
Cdd:pfam05424 1 ACAPPRRLHLCVKNLEKinSGNTTTTHDLLKAVILAAKYEGFSLWHKYKKKNT------KYGDIPSEFCRQMAYSFADIG 74
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 1907 NIFFGTDISSC--------QNIKNASENIKIILNKEKDEEKNEKQMINKWTNSYGPEIWEAMLCALvKIGAKKDDFTENY 1978
Cdd:pfam05424 75 DIIRGKDLYLGnnkkkkleENLKKIFKKIYEKLTSKGKDDGNYYQLREDWWEANREDIWKAMTCAL-TYGAKYFRKTCGD 153
|
170 180
....*....|....*....|....*
gi 1035522805 1979 GYNNVKFSDKSTTLEKFAQTPQFLR 2003
Cdd:pfam05424 154 GISPSTAGCNDDVPTYFDYVPQFLR 178
|
|
| PTZ00176 |
PTZ00176 |
erythrocyte membrane protein 1 (PfEMP1); Provisional |
1060-1727 |
2.29e-32 |
|
erythrocyte membrane protein 1 (PfEMP1); Provisional
Pssm-ID: 140204 [Multi-domain] Cd Length: 1317 Bit Score: 139.40 E-value: 2.29e-32
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 1060 CNSQIHR-----THNGACMPPRRQKLCVINLQYFTGETSDGLRKAfiecaaVETFFLWHKYKednngGEDLQNQlKSGKI 1134
Cdd:PTZ00176 105 CNSDKIRgnennSNAGACAPFRRQNMCDKNLEYLINKNTENTHDL------LGNVLVTAKYE-----GESIVNN-HPDKD 172
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 1135 PEEFKRQMFYTFGDYRDFLFGTDISKNHgKGSKLANKIDSLFP---------------PNNKKPGTLS-RQKWWNENGPK 1198
Cdd:PTZ00176 173 KSSVCTALARSFADIGDIVRGKDMFKRN-KHDNVENGLREVFKkiyeglknngarehyKEVKNGNYIKlREDWWTANRDQ 251
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 1199 IWNAMLCALSYNTN-EKTFKDKVH-SQLTTTYgySKIKFSDKSTTLEEFakrPQFLRWFTEWGEHFCKEHTKELAKLVTA 1276
Cdd:PTZ00176 252 VWKAMTCVAPENAYfRKTEADGIGiSSLILPY--SKCGRDTDPPVVDYI---PQRLRWMSEWSEYFCNVLNKEIDEMNNQ 326
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 1277 CP----HDTCTNE---GKKKVCSDACANYKTFIKQWREEYKKQSKKYTE----DKTAKKYDNHSVAKKA----KDAREYL 1341
Cdd:PTZ00176 327 CKdcemSRRCNNDtegEKCKKCKEQCQIFKELVSKWKNQFDKQSMKYMElynkASTNITKQNSSAPERGyrrnHRRRGYD 406
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 1342 EKT-LQNFCQSDRTNGECEYKCMKNISTKEQPTENKSpvGNTDSMPAS-----LDDEPKEVEGRCNCTPPpdackivkvi 1415
Cdd:PTZ00176 407 DDTnVQLFLKKVIENNECKVESLGKYLDKTSHCGNYN--FNYDNTPGSnrsnaFEITPEKFKKACKCKIP---------- 474
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 1416 fngksatDDIQGCRPKENY-------KPWNCTSSQFK--------SG-------HTGACMPPRRQKLCvINL---KTFEP 1470
Cdd:PTZ00176 475 -------NPLEKCPNEENKnvctrfdKVYSCTSLSFKndlsewnnSGvknkendNNGVLVPPRRRNLC-INLfskKDYKM 546
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 1471 KTSVELRNAFIKCAAIETHFLWKYYkeknnvkndkileSGYIPEDFKrMMYYTFGDYRDLCLDKDIGNDVSDVENYIKGV 1550
Cdd:PTZ00176 547 KDENDFKEDLLNAAFSQGKLLGKKY-------------SNYSNEAYE-AMKFSYADYSDIVKGTDMMNDLKKLNKELNTL 612
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 1551 LTDsTKNGGTEITPDNWWKKIEKEVWDGMLCALSynskersfKEDVRTQLTTKYpystvkfsggnnsTTLEEFAKRPQFL 1630
Cdd:PTZ00176 613 LKE-TEKGDISVDRKTWWDDNKNVVWNAMLCGYK--------TENENQQLNSSW-------------CNVPDDDNIDQFL 670
|
650 660 670 680 690 700 710 720
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 1631 RWFIEWSDYFCAEQAKRLVTLKEKCPDdtCTNGENKKN-------QCKTACENYKKWLKDWKDKYKIQSEKFDADKKNKK 1703
Cdd:PTZ00176 671 RWLTEWAQQYCKEKLIKAHIINTKCKD--IVEGRKHKSmvditdvECKRLFIDYEEWFRYRYNQWKGLSEKYIKIKKSKN 748
|
730 740
....*....|....*....|....*.
gi 1035522805 1704 YEKDPAANE--ASSARDYLNKALINL 1727
Cdd:PTZ00176 749 SGVNIPSEEcaASYVTKHCNGCICNL 774
|
|
| Duffy_binding |
pfam05424 |
Duffy binding domain; This domain is found in Plasmodium Duffy binding proteins. Plasmodium ... |
1-111 |
2.44e-32 |
|
Duffy binding domain; This domain is found in Plasmodium Duffy binding proteins. Plasmodium vivax and Plasmodium knowlesi merozoites invade human erythrocytes that express Duffy blood group surface determinants. The Duffy receptor family is localized in micronemes, an organelle found in all organizms of the phylum Apicomplexa. This family is closely associated on PfEMP1 proteins with PFEMP, pfam03011.
Pssm-ID: 428465 [Multi-domain] Cd Length: 178 Bit Score: 125.46 E-value: 2.44e-32
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 1 DIVRGKDLYLGKNEsekrtKEKLQGNLVNIFKKFKEKY--GDLKDVPIDDIREYWWALNRKDVWKALTCNAPYEAQYFIK 78
Cdd:pfam05424 75 DIIRGKDLYLGNNK-----KKKLEENLKKIFKKIYEKLtsKGKDDGNYYQLREDWWEANREDIWKAMTCALTYGAKYFRK 149
|
90 100 110
....*....|....*....|....*....|...
gi 1035522805 79 SSRDDLTFSNEHCghykNGDPLTNLDYVPQFLR 111
Cdd:pfam05424 150 TCGDGISPSTAGC----NDDVPTYFDYVPQFLR 178
|
|
| PTZ00176 |
PTZ00176 |
erythrocyte membrane protein 1 (PfEMP1); Provisional |
1445-2128 |
3.92e-29 |
|
erythrocyte membrane protein 1 (PfEMP1); Provisional
Pssm-ID: 140204 [Multi-domain] Cd Length: 1317 Bit Score: 128.61 E-value: 3.92e-29
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 1445 KSGHTGACMPPRRQKLCVINLKTF---EPKTSVELRNAFIKCAAIETHFLwkyykeknnVKNDKILESGYIPEDFKRmmy 1521
Cdd:PTZ00176 115 NNSNAGACAPFRRQNMCDKNLEYLinkNTENTHDLLGNVLVTAKYEGESI---------VNNHPDKDKSSVCTALAR--- 182
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 1522 yTFGDYRDLCLDKDI--GNDVSDVENYIKGVLTD---------------STKNGGTEITPDNWWKKIEKEVWDGMLCALS 1584
Cdd:PTZ00176 183 -SFADIGDIVRGKDMfkRNKHDNVENGLREVFKKiyeglknngarehykEVKNGNYIKLREDWWTANRDQVWKAMTCVAP 261
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 1585 YNSKERSFKEDVRTQLTTKYPYSTVkfsGGNNSTTLEEFAkrPQFLRWFIEWSDYFCAEQAKRLVTLKEKCPD------- 1657
Cdd:PTZ00176 262 ENAYFRKTEADGIGISSLILPYSKC---GRDTDPPVVDYI--PQRLRWMSEWSEYFCNVLNKEIDEMNNQCKDcemsrrc 336
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 1658 DTCTNGENKKnQCKTACENYKKWLKDWKDKYKIQSEKFdADKKNKKYEKDPAANEASSARDY--------------LNKA 1723
Cdd:PTZ00176 337 NNDTEGEKCK-KCKEQCQIFKELVSKWKNQFDKQSMKY-MELYNKASTNITKQNSSAPERGYrrnhrrrgydddtnVQLF 414
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 1724 LINLCPNGTCSCMNDSSREPKQLPDGN----------TDMPSSLDDTPSEYKQRCKCPEAPPKKE---EEKKDACTIATn 1790
Cdd:PTZ00176 415 LKKVIENNECKVESLGKYLDKTSHCGNynfnydntpgSNRSNAFEITPEKFKKACKCKIPNPLEKcpnEENKNVCTRFD- 493
|
410 420 430 440 450 460 470 480
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 1791 lvknnngkaKINGCGT---KTNVTypEWkyHNSSRLVRED---GVCMPPRRQKLCLYFLTKSN-NLNSEDDIRNNFITCA 1863
Cdd:PTZ00176 494 ---------KVYSCTSlsfKNDLS--EW--NNSGVKNKENdnnGVLVPPRRRNLCINLFSKKDyKMKDENDFKEDLLNAA 560
|
490 500 510 520 530 540 550 560
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 1864 AIETHFAWDRYKNKNKGADDQLKsgtipdefkrqmfYTFSDYRNIFFGTDISScqNIKNASENIKIILNKEKDEEKNEKQ 1943
Cdd:PTZ00176 561 FSQGKLLGKKYSNYSNEAYEAMK-------------FSYADYSDIVKGTDMMN--DLKKLNKELNTLLKETEKGDISVDR 625
|
570 580 590 600 610 620 630 640
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 1944 miNKWTNSYGPEIWEAMLCalvkiGAKKDDFTE--NYGYNNVKFSDksttlekfaQTPQFLRWFIEWGEEFCREREEKEK 2021
Cdd:PTZ00176 626 --KTWWDDNKNVVWNAMLC-----GYKTENENQqlNSSWCNVPDDD---------NIDQFLRWLTEWAQQYCKEKLIKAH 689
|
650 660 670 680 690 700 710 720
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 2022 AVKEVCTQVKEYEGCKNKKTSGGSPCNTACEAYEDYITKKKVEYTSQEGKFNREKSQGKQEYKDISNINAPDYLKKECLD 2101
Cdd:PTZ00176 690 IINTKCKDIVEGRKHKSMVDITDVECKRLFIDYEEWFRYRYNQWKGLSEKYIKIKKSKNSGVNIPSEECAASYVTKHCNG 769
|
730 740
....*....|....*....|....*..
gi 1035522805 2102 DTCNCMDKvqtikdywEQPHKTYNNSD 2128
Cdd:PTZ00176 770 CICNLRDM--------EDIHKNINNQN 788
|
|
| PTZ00176 |
PTZ00176 |
erythrocyte membrane protein 1 (PfEMP1); Provisional |
1-195 |
1.45e-23 |
|
erythrocyte membrane protein 1 (PfEMP1); Provisional
Pssm-ID: 140204 [Multi-domain] Cd Length: 1317 Bit Score: 110.51 E-value: 1.45e-23
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 1 DIVRGKDLYlgknesEKRTKEKLQGNLVNIFKKF---------KEKYGDLKDVPIDDIREYWWALNRKDVWKALTCNAPy 71
Cdd:PTZ00176 189 DIVRGKDMF------KRNKHDNVENGLREVFKKIyeglknngaREHYKEVKNGNYIKLREDWWTANRDQVWKAMTCVAP- 261
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 72 EAQYFIKSSRDDLTFSN-----EHCGHykNGDPLTnLDYVPQFLRWFEEWAEHFCLVRKYKLEKVKEACRgktggmycsh 146
Cdd:PTZ00176 262 ENAYFRKTEADGIGISSlilpySKCGR--DTDPPV-VDYIPQRLRWMSEWSEYFCNVLNKEIDEMNNQCK---------- 328
|
170 180 190 200
....*....|....*....|....*....|....*....|....*....
gi 1035522805 147 ngcDCEKTiGKIRHFVWDHKCNKCSIECGLYQNWMKDQKLEFEKQKEKY 195
Cdd:PTZ00176 329 ---DCEMS-RRCNNDTEGEKCKKCKEQCQIFKELVSKWKNQFDKQSMKY 373
|
|
| PTZ00176 |
PTZ00176 |
erythrocyte membrane protein 1 (PfEMP1); Provisional |
634-912 |
5.27e-19 |
|
erythrocyte membrane protein 1 (PfEMP1); Provisional
Pssm-ID: 140204 [Multi-domain] Cd Length: 1317 Bit Score: 95.49 E-value: 5.27e-19
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 634 WKHDNAVSKIHKN--VYMPPRRQHFCTSNLEYlingkyeailKIQKGKINHSFLGDVLLAAKYQAEDTMKDYKPKGDQEG 711
Cdd:PTZ00176 510 WNNSGVKNKENDNngVLVPPRRRNLCINLFSK----------KDYKMKDENDFKEDLLNAAFSQGKLLGKKYSNYSNEAY 579
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 712 KcrAIRYSFADLGDIIKGTDLWkgnSGEQKTQRNLQRIfgkIKEELPGDIQKKytgtkhlklRADWWEANRAKVWEAMKC 791
Cdd:PTZ00176 580 E--AMKFSYADYSDIVKGTDMM---NDLKKLNKELNTL---LKETEKGDISVD---------RKTWWDDNKNVVWNAMLC 642
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 792 HIGDLNDTSVHPSSSGNcgysdhTPLDDYIPQRLRWMTEWAEWYCKEQSRLYGELVEKCAG-CKGKQKCTEGDVDCGKCK 870
Cdd:PTZ00176 643 GYKTENENQQLNSSWCN------VPDDDNIDQFLRWLTEWAQQYCKEKLIKAHIINTKCKDiVEGRKHKSMVDITDVECK 716
|
250 260 270 280
....*....|....*....|....*....|....*....|..
gi 1035522805 871 PACENYKKFIKKWQPQWDKIRAKYKKIYEharvdiAANGGLN 912
Cdd:PTZ00176 717 RLFIDYEEWFRYRYNQWKGLSEKYIKIKK------SKNSGVN 752
|
|
| CIDR1_gamma |
pfam18562 |
Cysteine-Rich Interdomain Region 1 gamma; Rosetting is the capacity of infected RBCs to bind ... |
2794-2844 |
2.36e-18 |
|
Cysteine-Rich Interdomain Region 1 gamma; Rosetting is the capacity of infected RBCs to bind uninfected RBCs, which is consistently associated with severe malaria in African children. The rosette-forming PfEMP1 adhesins, namely IT4/R29, Palo Alto 89F5 VarO, 3D7/PF13_0003 and IT4/var60, belong to a specific sub-group called groupA/UpsA var genes and all four present a specific Duffy Binding-Like and and Cysteine-Rich Interdomain Region (DBL1alpha1-CIDR1gamma) double domain Head region found at the extracellular region of PfEMP1. This entry represents the CIDR1gamma domain which increases the binding affinity to VarO (Palo Alto VarO parasites).
Pssm-ID: 408346 Cd Length: 52 Bit Score: 80.78 E-value: 2.36e-18
10 20 30 40 50
....*....|....*....|....*....|....*....|....*....|..
gi 1035522805 2794 TEKLDMRVIDDSATGF-GDLQDCTDANIFKSIRKDVWKCGKVCGYNVCKPEN 2844
Cdd:pfam18562 1 TTTIDMLVSDNRGIKFeNDLKECKEAGIFKGIRKDQWKCGKVCGYDVCKLKN 52
|
|
| PFEMP |
pfam03011 |
PFEMP1 DBL domain; PfEMP1 (Plasmodium falciparum erythrocyte membrane protein) has been ... |
383-526 |
3.65e-14 |
|
PFEMP1 DBL domain; PfEMP1 (Plasmodium falciparum erythrocyte membrane protein) has been identified as the rosetting ligand of the malaria parasite P. falciparum. Rosetting is the adhesion of infected erythrocytes with uninfected erythrocytes in the vasculature of the infected organ, and is associated with severe malaria. PfEMP1 interacts with Complement Receptor One on uninfected erythrocytes to form rosettes. The extreme variation within these proteins and the grouping of var genes implies that var gene recombination preferentially occurs within var gene groups. These groups reflect a functional diversification that has evolved to cope with the varying conditions of transmission and host immune response met by the parasite. A recombination hotspot was uncovered between Duffy-binding-like (DBL) subdomains. Solution of the crystal structure of the N-terminal and first DBL region of PfEMP1 from the VarO variant of the PfEMP1 protein is found to be directly implicated in rosetting as the heparin-binding site.
Pssm-ID: 281064 Cd Length: 154 Bit Score: 72.53 E-value: 3.65e-14
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 383 LFHSWVRNLLIDTINWETDLRNCLNNTGITDCNDGCNNNCTCFYNWVKQKEKEWTNMMDLFTKEQKMPKKYYLNINDLFD 462
Cdd:pfam03011 1 LFKRWVEYFLEDSIKWRKKLKSCINNGKGKCCKKECKNDCECFKKWVEKKKEEWKKIKEHFLKQYKLKGLTGKTLDEYLD 80
|
90 100 110 120 130 140 150
....*....|....*....|....*....|....*....|....*....|....*....|....*....|..
gi 1035522805 463 SFFF---EVMDKLNQDETKWNKLKENLKKKIESSKKNAGTK-----DSEAAIKVLLDHLKEIATICKDNNTN 526
Cdd:pfam03011 81 LKSFletFLFYIDIKEAYGDVKELKKLEEILDEEGCSAGAEsaknnKNEDAIDKLLDKEEKKANNCKEKHKE 152
|
|
| PTZ00176 |
PTZ00176 |
erythrocyte membrane protein 1 (PfEMP1); Provisional |
2328-2680 |
1.73e-06 |
|
erythrocyte membrane protein 1 (PfEMP1); Provisional
Pssm-ID: 140204 [Multi-domain] Cd Length: 1317 Bit Score: 54.27 E-value: 1.73e-06
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 2328 DGAICVPPRRRKLYLHKMEGVDT----TTESLRKWFIETAAIEtfflwdrykkewehrnkktqdglpgvgvGNGDDENNP 2403
Cdd:PTZ00176 118 NAGACAPFRRQNMCDKNLEYLINknteNTHDLLGNVLVTAKYE----------------------------GESIVNNHP 169
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 2404 QKklqESGTIPIDFLRqmfyTLGDYRDILFGKNHIvigktpSVSAKDEMAKREEEIKNAIDNYFSKSGSTQTQP-----N 2478
Cdd:PTZ00176 170 DK---DKSSVCTALAR----SFADIGDIVRGKDMF------KRNKHDNVENGLREVFKKIYEGLKNNGAREHYKevkngN 236
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 2479 SDKDPKAWWNNNAKHIWKGMICAltyedngekgTPQvndslyNKFFgtqngnprlqpvtpgkppnpgtnTGTYKEKYDYE 2558
Cdd:PTZ00176 237 YIKLREDWWTANRDQVWKAMTCV----------APE------NAYF-----------------------RKTEADGIGIS 277
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 2559 NVTLkdeqsgggPKPTGGDSTlnNPKLTQFVlrPPYFRYLEEWGETFCKERKKRLDQIYRECKvdsddykcsgygeDCKN 2638
Cdd:PTZ00176 278 SLIL--------PYSKCGRDT--DPPVVDYI--PQRLRWMSEWSEYFCNVLNKEIDEMNNQCK-------------DCEM 332
|
330 340 350 360
....*....|....*....|....*....|....*....|..
gi 1035522805 2639 NLREKYDTvPSLECPDCAKHCSFYKKWIKKKRTEFNKQSNVY 2680
Cdd:PTZ00176 333 SRRCNNDT-EGEKCKKCKEQCQIFKELVSKWKNQFDKQSMKY 373
|
|
| PTZ00176 |
PTZ00176 |
erythrocyte membrane protein 1 (PfEMP1); Provisional |
1-136 |
3.09e-06 |
|
erythrocyte membrane protein 1 (PfEMP1); Provisional
Pssm-ID: 140204 [Multi-domain] Cd Length: 1317 Bit Score: 53.50 E-value: 3.09e-06
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1035522805 1 DIVRGKDLYlgkNESEKRTKEklqgnlVNIFKKFKEKygdlKDVPIDdiREYWWALNRKDVWKALTCNapyeaqyfIKSS 80
Cdd:PTZ00176 591 DIVKGTDMM---NDLKKLNKE------LNTLLKETEK----GDISVD--RKTWWDDNKNVVWNAMLCG--------YKTE 647
|
90 100 110 120 130
....*....|....*....|....*....|....*....|....*....|....*.
gi 1035522805 81 RDDLTFSNEHCGhykngdpLTNLDYVPQFLRWFEEWAEHFCLVRKYKLEKVKEACR 136
Cdd:PTZ00176 648 NENQQLNSSWCN-------VPDDDNIDQFLRWLTEWAQQYCKEKLIKAHIINTKCK 696
|
|
| |
