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Conserved domains on  [gi|74186837|dbj|BAE20491|]
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unnamed protein product, partial [Mus musculus]

Protein Classification

RhoGEF domain-containing protein( domain architecture ID 10457370)

RhoGEF domain-containing protein contains guanine nucleotide exchange factor (GEF) activity to regulate small GTPases in the Rho family; may contain a pleckstrin homology (PH) domain

CATH:  1.20.900.10|2.30.29.30
Gene Ontology:  GO:0051056|GO:0005085|GO:0005515
PubMed:  16393146|11911885|15817389|11738596|23303910|22728242
SCOP:  4001108|4002395

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
RhoGEF pfam00621
RhoGEF domain; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases Also called ...
 116-290 1.42e-52

RhoGEF domain; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases Also called Dbl-homologous (DH) domain. It appears that pfam00169 domains invariably occur C-terminal to RhoGEF/DH domains.


:

Pssm-ID: 459876 [Multi-domain]  Cd Length: 176  Bit Score: 171.71  E-value: 1.42e-52
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 74186837   116 VVQEILETERTYVQDLKSIVEGYLECIRDqtKLPLVTEDRAALFGNIQDIYHFN-SELLQDLENCENDPVAIAECFVSKS 194
Cdd:pfam00621   1 VIKELLQTERSYVRDLEILVEVFLPPNSK--PLSESEEEIKTIFSNIEEIYELHrQLLLEELLKEWISIQRIGDIFLKFA 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 74186837   195 EEFHIYTQYCTNYPRSVAVLTECMR-NKILTKFFRERQETLR-HSLPLGSYLLKPVQRILKYHLLLHEIENHLDKATEGY 272
Cdd:pfam00621  79 PGFKVYSTYCSNYPKALKLLKKLLKkNPKFRAFLEELEANPEcRGLDLNSFLIKPVQRIPRYPLLLKELLKHTPPDHPDY 158

                         170
                  ....*....|....*...
gi 74186837   273 DVVLDAIDTMQRVAWHIN 290
Cdd:pfam00621 159 EDLKKALEAIKEVAKQIN 176
PH-like super family cl17171
Pleckstrin homology-like domain; The PH-like family includes the PH domain, both the Shc-like ...
 272-339 7.05e-33

Pleckstrin homology-like domain; The PH-like family includes the PH domain, both the Shc-like and IRS-like PTB domains, the ran-binding domain, the EVH1 domain, a domain in neurobeachin and the third domain of FERM. All of these domains have a PH fold, but lack significant sequence similarity. They are generally involved in targeting to protein to the appropriate cellular location or interacting with a binding partner. This domain family possesses multiple functions including the ability to bind inositol phosphates and to other proteins.


The actual alignment was detected with superfamily member cd13243:

Pssm-ID: 473070 [Multi-domain]  Cd Length: 147  Bit Score: 119.38  E-value: 7.05e-33
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 74186837 272 YDVVLDAIDTMQRVAWHINDMKRKHEHAVRLQEIQSLLTNWEGPDLTSYGELVLEGTFRIQKAKKERT 339
Cdd:cd13243   1 RSVVEEALDTMTQVAWHINDMKRKHEHAVRVQEIQSLLDGWEGPELTTYGDLVLEGTFRMAGAKNERL 68
 
Name Accession Description Interval E-value
RhoGEF pfam00621
RhoGEF domain; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases Also called ...
 116-290 1.42e-52

RhoGEF domain; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases Also called Dbl-homologous (DH) domain. It appears that pfam00169 domains invariably occur C-terminal to RhoGEF/DH domains.


Pssm-ID: 459876 [Multi-domain]  Cd Length: 176  Bit Score: 171.71  E-value: 1.42e-52
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 74186837   116 VVQEILETERTYVQDLKSIVEGYLECIRDqtKLPLVTEDRAALFGNIQDIYHFN-SELLQDLENCENDPVAIAECFVSKS 194
Cdd:pfam00621   1 VIKELLQTERSYVRDLEILVEVFLPPNSK--PLSESEEEIKTIFSNIEEIYELHrQLLLEELLKEWISIQRIGDIFLKFA 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 74186837   195 EEFHIYTQYCTNYPRSVAVLTECMR-NKILTKFFRERQETLR-HSLPLGSYLLKPVQRILKYHLLLHEIENHLDKATEGY 272
Cdd:pfam00621  79 PGFKVYSTYCSNYPKALKLLKKLLKkNPKFRAFLEELEANPEcRGLDLNSFLIKPVQRIPRYPLLLKELLKHTPPDHPDY 158

                         170
                  ....*....|....*...
gi 74186837   273 DVVLDAIDTMQRVAWHIN 290
Cdd:pfam00621 159 EDLKKALEAIKEVAKQIN 176
RhoGEF cd00160
Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous ...
 114-290 1.03e-49

Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.


Pssm-ID: 238091 [Multi-domain]  Cd Length: 181  Bit Score: 164.39  E-value: 1.03e-49
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 74186837 114 DRVVQEILETERTYVQDLKSIVEGYLECIrDQTKLPLVTEDRAALFGNIQDIYHFNSELLQDLENC----ENDPVAIAEC 189
Cdd:cd00160   2 QEVIKELLQTERNYVRDLKLLVEVFLKPL-DKELLPLSPEEVELLFGNIEEIYEFHRIFLKSLEERveewDKSGPRIGDV 80

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 74186837 190 FVSKSEEFHIYTQYCTNYPRSVAVLTECMRNKilTKF--FRERQETLRHSLPLGSYLLKPVQRILKYHLLLHEIENHLDK 267
Cdd:cd00160  81 FLKLAPFFKIYSEYCSNHPDALELLKKLKKFN--KFFqeFLEKAESECGRLKLESLLLKPVQRLTKYPLLLKELLKHTPD 158

                       170       180
                ....*....|....*....|...
gi 74186837 268 ATEGYDVVLDAIDTMQRVAWHIN 290
Cdd:cd00160 159 GHEDREDLKKALEAIKEVASQVN 181
RhoGEF smart00325
Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Guanine nucleotide exchange ...
 116-291 8.57e-48

Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains. Improved coverage.


Pssm-ID: 214619 [Multi-domain]  Cd Length: 180  Bit Score: 159.39  E-value: 8.57e-48
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 74186837    116 VVQEILETERTYVQDLKSIVEGYLECIRDQTKLpLVTEDRAALFGNIQDIYHFNSELLQDLENC----ENDPVAIAECFV 191
Cdd:smart00325   1 VLKELLQTERNYVRDLKLLVEVFLKPLKKELKL-LSPNELETLFGNIEEIYEFHRDFLDELEERieewDDSVERIGDVFL 79

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 74186837    192 SKSEEFHIYTQYCTNYPRSVAVLTECMRNKILTKFFRERQETLRH-SLPLGSYLLKPVQRILKYHLLLHEIENHLDKATE 270
Cdd:smart00325  80 KLEEFFKIYSEYCSNHPDALELLKKLKKNPRFQKFLKEIESSPQCrRLTLESLLLKPVQRLTKYPLLLKELLKHTPEDHE 159

                          170       180
                   ....*....|....*....|.
gi 74186837    271 GYDVVLDAIDTMQRVAWHIND 291
Cdd:smart00325 160 DREDLKKALKAIKELANQVNE 180
PH_PLEKHG1_G2_G3 cd13243
Pleckstrin homology domain-containing family G members 1, 2, and 3 pleckstrin homology (PH) ...
 272-339 7.05e-33

Pleckstrin homology domain-containing family G members 1, 2, and 3 pleckstrin homology (PH) domain; PLEKHG1 (also called ARHGEF41), PLEKHG2 (also called ARHGEF42 or CLG/common-site lymphoma/leukemia guanine nucleotide exchange factor2), and PLEKHG3 (also called ARHGEF43) have RhoGEF DH/double-homology domains in tandem with a PH domain which is involved in phospholipid binding. They function as a guanine nucleotide exchange factor (GEF) and are involved in the regulation of Rho protein signal transduction. Mutations in PLEKHG1 have been associated panic disorder (PD), an anxiety disorder characterized by panic attacks and anticipatory anxiety. PH domains have diverse functions, but in general are involved in targeting proteins to the appropriate cellular location or in the interaction with a binding partner. They share little sequence conservation, but all have a common fold, which is electrostatically polarized. Less than 10% of PH domains bind phosphoinositide phosphates (PIPs) with high affinity and specificity. PH domains are distinguished from other PIP-binding domains by their specific high-affinity binding to PIPs with two vicinal phosphate groups: PtdIns(3,4)P2, PtdIns(4,5)P2 or PtdIns(3,4,5)P3 which results in targeting some PH domain proteins to the plasma membrane. A few display strong specificity in lipid binding. Any specificity is usually determined by loop regions or insertions in the N-terminus of the domain, which are not conserved across all PH domains. PH domains are found in cellular signaling proteins such as serine/threonine kinase, tyrosine kinases, regulators of G-proteins, endocytotic GTPases, adaptors, as well as cytoskeletal associated molecules and in lipid associated enzymes.


Pssm-ID: 270063 [Multi-domain]  Cd Length: 147  Bit Score: 119.38  E-value: 7.05e-33
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 74186837 272 YDVVLDAIDTMQRVAWHINDMKRKHEHAVRLQEIQSLLTNWEGPDLTSYGELVLEGTFRIQKAKKERT 339
Cdd:cd13243   1 RSVVEEALDTMTQVAWHINDMKRKHEHAVRVQEIQSLLDGWEGPELTTYGDLVLEGTFRMAGAKNERL 68
ROM1 COG5422
RhoGEF, Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases [Signal transduction ...
 75-280 7.78e-05

RhoGEF, Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases [Signal transduction mechanisms];


Pssm-ID: 227709 [Multi-domain]  Cd Length: 1175  Bit Score: 44.50  E-value: 7.78e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 74186837   75 PQNMNGVSEKQ--PRTPWrmdTSGTPQTTADSATSPKLLYVDrVVQEILETERTYVQDLksivegylECIRDQTKLPLVT 152
Cdd:COG5422  449 KRNSSLALDKFdeEKNLW---TLSVPKEVWESLPKQEIKRQE-AIYEVIYTERDFVKDL--------EYLRDTWIKPLEE 516

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 74186837  153 EDRAA----------LFGNIQDIYHFNSELLQDLENCEN-DPVA--IAECFVSKSEEFHIYTQYCTNYPRSVAVLTEcmR 219
Cdd:COG5422  517 SNIIPenarrnfikhVFANINEIYAVNSKLLKALTNRQClSPIVngIADIFLDYVPKFEPFIKYGASQPYAKYEFER--E 594

                        170       180       190       200       210       220
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 74186837  220 NKILTKFFRERQETLRHSLP----LGSYLLKPVQRILKYHLLLHEIENHLDKATEGYDVVLDAID 280
Cdd:COG5422  595 KSVNPNFARFDHEVERLDESrkleLDGYLTKPTTRLARYPLLLEEVLKFTDPDNPDTEDIPKVID 659
 
Name Accession Description Interval E-value
RhoGEF pfam00621
RhoGEF domain; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases Also called ...
 116-290 1.42e-52

RhoGEF domain; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases Also called Dbl-homologous (DH) domain. It appears that pfam00169 domains invariably occur C-terminal to RhoGEF/DH domains.


Pssm-ID: 459876 [Multi-domain]  Cd Length: 176  Bit Score: 171.71  E-value: 1.42e-52
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 74186837   116 VVQEILETERTYVQDLKSIVEGYLECIRDqtKLPLVTEDRAALFGNIQDIYHFN-SELLQDLENCENDPVAIAECFVSKS 194
Cdd:pfam00621   1 VIKELLQTERSYVRDLEILVEVFLPPNSK--PLSESEEEIKTIFSNIEEIYELHrQLLLEELLKEWISIQRIGDIFLKFA 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 74186837   195 EEFHIYTQYCTNYPRSVAVLTECMR-NKILTKFFRERQETLR-HSLPLGSYLLKPVQRILKYHLLLHEIENHLDKATEGY 272
Cdd:pfam00621  79 PGFKVYSTYCSNYPKALKLLKKLLKkNPKFRAFLEELEANPEcRGLDLNSFLIKPVQRIPRYPLLLKELLKHTPPDHPDY 158

                         170
                  ....*....|....*...
gi 74186837   273 DVVLDAIDTMQRVAWHIN 290
Cdd:pfam00621 159 EDLKKALEAIKEVAKQIN 176
RhoGEF cd00160
Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous ...
 114-290 1.03e-49

Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.


Pssm-ID: 238091 [Multi-domain]  Cd Length: 181  Bit Score: 164.39  E-value: 1.03e-49
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 74186837 114 DRVVQEILETERTYVQDLKSIVEGYLECIrDQTKLPLVTEDRAALFGNIQDIYHFNSELLQDLENC----ENDPVAIAEC 189
Cdd:cd00160   2 QEVIKELLQTERNYVRDLKLLVEVFLKPL-DKELLPLSPEEVELLFGNIEEIYEFHRIFLKSLEERveewDKSGPRIGDV 80

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 74186837 190 FVSKSEEFHIYTQYCTNYPRSVAVLTECMRNKilTKF--FRERQETLRHSLPLGSYLLKPVQRILKYHLLLHEIENHLDK 267
Cdd:cd00160  81 FLKLAPFFKIYSEYCSNHPDALELLKKLKKFN--KFFqeFLEKAESECGRLKLESLLLKPVQRLTKYPLLLKELLKHTPD 158

                       170       180
                ....*....|....*....|...
gi 74186837 268 ATEGYDVVLDAIDTMQRVAWHIN 290
Cdd:cd00160 159 GHEDREDLKKALEAIKEVASQVN 181
RhoGEF smart00325
Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Guanine nucleotide exchange ...
 116-291 8.57e-48

Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains. Improved coverage.


Pssm-ID: 214619 [Multi-domain]  Cd Length: 180  Bit Score: 159.39  E-value: 8.57e-48
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 74186837    116 VVQEILETERTYVQDLKSIVEGYLECIRDQTKLpLVTEDRAALFGNIQDIYHFNSELLQDLENC----ENDPVAIAECFV 191
Cdd:smart00325   1 VLKELLQTERNYVRDLKLLVEVFLKPLKKELKL-LSPNELETLFGNIEEIYEFHRDFLDELEERieewDDSVERIGDVFL 79

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 74186837    192 SKSEEFHIYTQYCTNYPRSVAVLTECMRNKILTKFFRERQETLRH-SLPLGSYLLKPVQRILKYHLLLHEIENHLDKATE 270
Cdd:smart00325  80 KLEEFFKIYSEYCSNHPDALELLKKLKKNPRFQKFLKEIESSPQCrRLTLESLLLKPVQRLTKYPLLLKELLKHTPEDHE 159

                          170       180
                   ....*....|....*....|.
gi 74186837    271 GYDVVLDAIDTMQRVAWHIND 291
Cdd:smart00325 160 DREDLKKALKAIKELANQVNE 180
PH_PLEKHG1_G2_G3 cd13243
Pleckstrin homology domain-containing family G members 1, 2, and 3 pleckstrin homology (PH) ...
 272-339 7.05e-33

Pleckstrin homology domain-containing family G members 1, 2, and 3 pleckstrin homology (PH) domain; PLEKHG1 (also called ARHGEF41), PLEKHG2 (also called ARHGEF42 or CLG/common-site lymphoma/leukemia guanine nucleotide exchange factor2), and PLEKHG3 (also called ARHGEF43) have RhoGEF DH/double-homology domains in tandem with a PH domain which is involved in phospholipid binding. They function as a guanine nucleotide exchange factor (GEF) and are involved in the regulation of Rho protein signal transduction. Mutations in PLEKHG1 have been associated panic disorder (PD), an anxiety disorder characterized by panic attacks and anticipatory anxiety. PH domains have diverse functions, but in general are involved in targeting proteins to the appropriate cellular location or in the interaction with a binding partner. They share little sequence conservation, but all have a common fold, which is electrostatically polarized. Less than 10% of PH domains bind phosphoinositide phosphates (PIPs) with high affinity and specificity. PH domains are distinguished from other PIP-binding domains by their specific high-affinity binding to PIPs with two vicinal phosphate groups: PtdIns(3,4)P2, PtdIns(4,5)P2 or PtdIns(3,4,5)P3 which results in targeting some PH domain proteins to the plasma membrane. A few display strong specificity in lipid binding. Any specificity is usually determined by loop regions or insertions in the N-terminus of the domain, which are not conserved across all PH domains. PH domains are found in cellular signaling proteins such as serine/threonine kinase, tyrosine kinases, regulators of G-proteins, endocytotic GTPases, adaptors, as well as cytoskeletal associated molecules and in lipid associated enzymes.


Pssm-ID: 270063 [Multi-domain]  Cd Length: 147  Bit Score: 119.38  E-value: 7.05e-33
                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 74186837 272 YDVVLDAIDTMQRVAWHINDMKRKHEHAVRLQEIQSLLTNWEGPDLTSYGELVLEGTFRIQKAKKERT 339
Cdd:cd13243   1 RSVVEEALDTMTQVAWHINDMKRKHEHAVRVQEIQSLLDGWEGPELTTYGDLVLEGTFRMAGAKNERL 68
ROM1 COG5422
RhoGEF, Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases [Signal transduction ...
 75-280 7.78e-05

RhoGEF, Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases [Signal transduction mechanisms];


Pssm-ID: 227709 [Multi-domain]  Cd Length: 1175  Bit Score: 44.50  E-value: 7.78e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 74186837   75 PQNMNGVSEKQ--PRTPWrmdTSGTPQTTADSATSPKLLYVDrVVQEILETERTYVQDLksivegylECIRDQTKLPLVT 152
Cdd:COG5422  449 KRNSSLALDKFdeEKNLW---TLSVPKEVWESLPKQEIKRQE-AIYEVIYTERDFVKDL--------EYLRDTWIKPLEE 516

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 74186837  153 EDRAA----------LFGNIQDIYHFNSELLQDLENCEN-DPVA--IAECFVSKSEEFHIYTQYCTNYPRSVAVLTEcmR 219
Cdd:COG5422  517 SNIIPenarrnfikhVFANINEIYAVNSKLLKALTNRQClSPIVngIADIFLDYVPKFEPFIKYGASQPYAKYEFER--E 594

                        170       180       190       200       210       220
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 74186837  220 NKILTKFFRERQETLRHSLP----LGSYLLKPVQRILKYHLLLHEIENHLDKATEGYDVVLDAID 280
Cdd:COG5422  595 KSVNPNFARFDHEVERLDESrkleLDGYLTKPTTRLARYPLLLEEVLKFTDPDNPDTEDIPKVID 659
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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