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Conserved domains on  [gi|943492703|ref|NP_001001399|]
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translocon-associated protein subunit beta precursor [Danio rerio]

Protein Classification

translocon-associated protein subunit beta( domain architecture ID 12065704)

translocon-associated protein subunit beta is part of a complex whose function is to bind calcium to the ER membrane and thereby regulate the retention of ER resident proteins

Gene Ontology:  GO:0006613
PubMed:  28218252

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
TRAP_beta pfam05753
Translocon-associated protein beta (TRAPB); This family consists of several eukaryotic ...
 7-182 1.57e-85

Translocon-associated protein beta (TRAPB); This family consists of several eukaryotic translocon-associated protein beta (TRAPB) or signal sequence receptor beta subunit (SSR-beta) proteins. The normal translocation of nascent polypeptides into the lumen of the endoplasmic reticulum (ER) is thought to be aided in part by a translocon-associated protein (TRAP) complex consisting of 4 protein subunits. The association of mature proteins with the ER and Golgi, or other intracellular locales, such as lysosomes, depends on the initial targeting of the nascent polypeptide to the ER membrane. A similar scenario must also exist for proteins destined for secretion.


:

Pssm-ID: 399046  Cd Length: 178  Bit Score: 249.57  E-value: 1.57e-85
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 943492703    7 CIFALVAVVGLVAGEEGARLLASKSLLNRYAVEGRDLTLQYSIYNVGTSAALEVELSDDSFPPEDFGIVSGMLNVKWDRI 86
Cdd:pfam05753   1 LALALLALAALAQDDAPARLLVKKKILNRYLVEGRDLTVTYTIYNVGSSAAYDVSLVDNSFPEEAFEVVSGSLSASWERL 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 943492703   87 APASNVSHTVVLRPLKAGYFNFTSASVSYLAQE-GGQVVVGYTSAPGQGGILAQREFDRRFSPHYLDWAAFGVMTLPSIG 165
Cdd:pfam05753  81 APGSNVSHIFVVEPKKSGYFNFTAAEVTYRPSEdGAELQVAYSSPPGEGGIFAERDYGKKFSLHLLDWVAFAVMTLPSLG 160

                         170
                  ....*....|....*..
gi 943492703  166 IPLLLWYSSKRKYDSPK 182
Cdd:pfam05753 161 IPYLLWWVSKSKYETSK 177
 
Name Accession Description Interval E-value
TRAP_beta pfam05753
Translocon-associated protein beta (TRAPB); This family consists of several eukaryotic ...
 7-182 1.57e-85

Translocon-associated protein beta (TRAPB); This family consists of several eukaryotic translocon-associated protein beta (TRAPB) or signal sequence receptor beta subunit (SSR-beta) proteins. The normal translocation of nascent polypeptides into the lumen of the endoplasmic reticulum (ER) is thought to be aided in part by a translocon-associated protein (TRAP) complex consisting of 4 protein subunits. The association of mature proteins with the ER and Golgi, or other intracellular locales, such as lysosomes, depends on the initial targeting of the nascent polypeptide to the ER membrane. A similar scenario must also exist for proteins destined for secretion.


Pssm-ID: 399046  Cd Length: 178  Bit Score: 249.57  E-value: 1.57e-85
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 943492703    7 CIFALVAVVGLVAGEEGARLLASKSLLNRYAVEGRDLTLQYSIYNVGTSAALEVELSDDSFPPEDFGIVSGMLNVKWDRI 86
Cdd:pfam05753   1 LALALLALAALAQDDAPARLLVKKKILNRYLVEGRDLTVTYTIYNVGSSAAYDVSLVDNSFPEEAFEVVSGSLSASWERL 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 943492703   87 APASNVSHTVVLRPLKAGYFNFTSASVSYLAQE-GGQVVVGYTSAPGQGGILAQREFDRRFSPHYLDWAAFGVMTLPSIG 165
Cdd:pfam05753  81 APGSNVSHIFVVEPKKSGYFNFTAAEVTYRPSEdGAELQVAYSSPPGEGGIFAERDYGKKFSLHLLDWVAFAVMTLPSLG 160

                         170
                  ....*....|....*..
gi 943492703  166 IPLLLWYSSKRKYDSPK 182
Cdd:pfam05753 161 IPYLLWWVSKSKYETSK 177
START_STARD3-like cd08906
Cholesterol-binding START domain of mammalian STARD3 and related proteins; This subgroup ...
 87-168 2.88e-03

Cholesterol-binding START domain of mammalian STARD3 and related proteins; This subgroup includes the steroidogenic acute regulatory protein (StAR)-related lipid transfer (START) domains of STARD3 (also known as metastatic lymph node 64/MLN64) and related proteins. It belongs to the START domain family, and in turn to the SRPBCC (START/RHO_alpha_C/PITP/Bet_v1/CoxG/CalC) domain superfamily of proteins that bind hydrophobic ligands. SRPBCC domains have a deep hydrophobic ligand-binding pocket. STARD3 has a high affinity for cholesterol. It may function in trafficking endosomal cholesterol to a cytosolic acceptor or membrane. In addition to having a cytoplasmic START cholesterol-binding domain, STARD3 also contains an N-terminal MENTAL cholesterol-binding and protein-protein interaction domain. The MENTAL domain contains transmembrane helices and anchors MLN64 to endosome membranes. The gene encoding STARD3 is overexpressed in about 25% of breast cancers.


Pssm-ID: 176915  Cd Length: 209  Bit Score: 37.15  E-value: 2.88e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 943492703  87 APASNVSHTVVLRPLKAGYFNFTSASVSYLAQEGGQVVVGY-TSAPGQGGILAQREF--DRRFSPHYLDWAAFGVMTLPS 163
Cdd:cd08906   59 CPAELVYQEVILQPEKMVLWNKTVSACQVLQRVDDNTLVSYdVAAGAAGGVVSPRDFvnVRRIERRRDRYVSAGISTTHS 138


                 ....*
gi 943492703 164 IGIPL 168
Cdd:cd08906  139 HKPPL 143
 
Name Accession Description Interval E-value
TRAP_beta pfam05753
Translocon-associated protein beta (TRAPB); This family consists of several eukaryotic ...
 7-182 1.57e-85

Translocon-associated protein beta (TRAPB); This family consists of several eukaryotic translocon-associated protein beta (TRAPB) or signal sequence receptor beta subunit (SSR-beta) proteins. The normal translocation of nascent polypeptides into the lumen of the endoplasmic reticulum (ER) is thought to be aided in part by a translocon-associated protein (TRAP) complex consisting of 4 protein subunits. The association of mature proteins with the ER and Golgi, or other intracellular locales, such as lysosomes, depends on the initial targeting of the nascent polypeptide to the ER membrane. A similar scenario must also exist for proteins destined for secretion.


Pssm-ID: 399046  Cd Length: 178  Bit Score: 249.57  E-value: 1.57e-85
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 943492703    7 CIFALVAVVGLVAGEEGARLLASKSLLNRYAVEGRDLTLQYSIYNVGTSAALEVELSDDSFPPEDFGIVSGMLNVKWDRI 86
Cdd:pfam05753   1 LALALLALAALAQDDAPARLLVKKKILNRYLVEGRDLTVTYTIYNVGSSAAYDVSLVDNSFPEEAFEVVSGSLSASWERL 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 943492703   87 APASNVSHTVVLRPLKAGYFNFTSASVSYLAQE-GGQVVVGYTSAPGQGGILAQREFDRRFSPHYLDWAAFGVMTLPSIG 165
Cdd:pfam05753  81 APGSNVSHIFVVEPKKSGYFNFTAAEVTYRPSEdGAELQVAYSSPPGEGGIFAERDYGKKFSLHLLDWVAFAVMTLPSLG 160

                         170
                  ....*....|....*..
gi 943492703  166 IPLLLWYSSKRKYDSPK 182
Cdd:pfam05753 161 IPYLLWWVSKSKYETSK 177
START_STARD3-like cd08906
Cholesterol-binding START domain of mammalian STARD3 and related proteins; This subgroup ...
 87-168 2.88e-03

Cholesterol-binding START domain of mammalian STARD3 and related proteins; This subgroup includes the steroidogenic acute regulatory protein (StAR)-related lipid transfer (START) domains of STARD3 (also known as metastatic lymph node 64/MLN64) and related proteins. It belongs to the START domain family, and in turn to the SRPBCC (START/RHO_alpha_C/PITP/Bet_v1/CoxG/CalC) domain superfamily of proteins that bind hydrophobic ligands. SRPBCC domains have a deep hydrophobic ligand-binding pocket. STARD3 has a high affinity for cholesterol. It may function in trafficking endosomal cholesterol to a cytosolic acceptor or membrane. In addition to having a cytoplasmic START cholesterol-binding domain, STARD3 also contains an N-terminal MENTAL cholesterol-binding and protein-protein interaction domain. The MENTAL domain contains transmembrane helices and anchors MLN64 to endosome membranes. The gene encoding STARD3 is overexpressed in about 25% of breast cancers.


Pssm-ID: 176915  Cd Length: 209  Bit Score: 37.15  E-value: 2.88e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 943492703  87 APASNVSHTVVLRPLKAGYFNFTSASVSYLAQEGGQVVVGY-TSAPGQGGILAQREF--DRRFSPHYLDWAAFGVMTLPS 163
Cdd:cd08906   59 CPAELVYQEVILQPEKMVLWNKTVSACQVLQRVDDNTLVSYdVAAGAAGGVVSPRDFvnVRRIERRRDRYVSAGISTTHS 138


                 ....*
gi 943492703 164 IGIPL 168
Cdd:cd08906  139 HKPPL 143
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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