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Conserved domains on  [gi|71984408|ref|NP_001023059|]
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Vps8 domain-containing protein [Caenorhabditis elegans]

Protein Classification

ANAPC4_WD40 and Vps8 domain-containing protein( domain architecture ID 11677268)

ANAPC4_WD40 and Vps8 domain-containing protein

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Vps8 pfam12816
Golgi CORVET complex core vacuolar protein 8; Vps8 is one of the Golgi complex components ...
 549-727 2.66e-56

Golgi CORVET complex core vacuolar protein 8; Vps8 is one of the Golgi complex components necessary for vacuolar sorting. Eukaryotic cells contain a highly dynamic endo-membrane system, in which individual organelles keep their identity despite continuous vesicle generation and fusion. Vesicles that bud from a donor membrane are targeted and delivered to each individual organelle, where they release their cargo after fusion with the acceptor membrane. Vps8 is the core component of the endosomal tethering complex CORVET (class C core vacuole/endosome tethering). Vps8 co-operates with Vps21-GTP to mediate endosomal clustering in a reaction that is dependent on Vps3. Vps8 is the only CORVET subunit that is enriched on late endosomes, suggesting that it is a marker for the maturation of late endosomes. Late endosomes form intralumenal vesicles, and the resulting multivesicular bodies fuse with the vacuole to release their cargoes.


:

Pssm-ID: 463718  Cd Length: 194  Bit Score: 193.54  E-value: 2.66e-56
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 71984408    549 VFLDHLDEYVLDGALIDPPPPLVNEYLQHLAAEGHFSQFQSAVVRFPIHTLDLHTVMSICKQNSIYDGIIYVNNKALNDY 628
Cdd:pfam12816    1 IFLETLEPYILSGRIRSLPPEVVKALVEHYASKGRLSRLEELICHLDPSSLDIDQVVKLCKKHGLYDALIYVWNRALNDY 80

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 71984408    629 ITPLENMLAEMRTFAHRDM-FSDSEQILGNKVLVYLNCCLSGMAYPFG-ALDEENRKRVPLETFRCI----------SSL 696
Cdd:pfam12816   81 VTPLVELLKLIRPLLNEGSdLEEDDEENANKVFPYLSYILTGRQYPTGeGLPEDEASKAKSEIYSFLfsgtsiewppGSG 160

                          170       180       190
                   ....*....|....*....|....*....|.
gi 71984408    697 KEKDGSESDEQYPYLRLLLKYDPQQFLNVVS 727
Cdd:pfam12816  161 TTDPDSDDEPSFPYLRLLLKFDAREFLSVLN 191
ANAPC4_WD40 super family cl14918
Anaphase-promoting complex subunit 4 WD40 domain; Apc4 contains an N-terminal propeller-shaped ...
 110-176 1.10e-05

Anaphase-promoting complex subunit 4 WD40 domain; Apc4 contains an N-terminal propeller-shaped WD40 domain.The N-terminus of Afi1 serves to stabilize the union between Apc4 and Apc5, both of which lie towards the bottom-front of the APC,


The actual alignment was detected with superfamily member pfam12894:

Pssm-ID: 403945 [Multi-domain]  Cd Length: 91  Bit Score: 44.96  E-value: 1.10e-05
                           10        20        30        40        50        60        70
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 71984408    110 IAVVTSKGQLLLFDLNGnlnqfHR-----GDETMGGASCVTFSLDGKFLAIGLQKGAIKIMTIDN-KLYHMID 176
Cdd:pfam12894   10 IALATEDGELLLHRLNW-----QRvwtlsPDKEDLEVTSLAWRPDGKLLAVGYSDGTVRLLDAENgKIVHHFS 77
 
Name Accession Description Interval E-value
Vps8 pfam12816
Golgi CORVET complex core vacuolar protein 8; Vps8 is one of the Golgi complex components ...
 549-727 2.66e-56

Golgi CORVET complex core vacuolar protein 8; Vps8 is one of the Golgi complex components necessary for vacuolar sorting. Eukaryotic cells contain a highly dynamic endo-membrane system, in which individual organelles keep their identity despite continuous vesicle generation and fusion. Vesicles that bud from a donor membrane are targeted and delivered to each individual organelle, where they release their cargo after fusion with the acceptor membrane. Vps8 is the core component of the endosomal tethering complex CORVET (class C core vacuole/endosome tethering). Vps8 co-operates with Vps21-GTP to mediate endosomal clustering in a reaction that is dependent on Vps3. Vps8 is the only CORVET subunit that is enriched on late endosomes, suggesting that it is a marker for the maturation of late endosomes. Late endosomes form intralumenal vesicles, and the resulting multivesicular bodies fuse with the vacuole to release their cargoes.


Pssm-ID: 463718  Cd Length: 194  Bit Score: 193.54  E-value: 2.66e-56
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 71984408    549 VFLDHLDEYVLDGALIDPPPPLVNEYLQHLAAEGHFSQFQSAVVRFPIHTLDLHTVMSICKQNSIYDGIIYVNNKALNDY 628
Cdd:pfam12816    1 IFLETLEPYILSGRIRSLPPEVVKALVEHYASKGRLSRLEELICHLDPSSLDIDQVVKLCKKHGLYDALIYVWNRALNDY 80

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 71984408    629 ITPLENMLAEMRTFAHRDM-FSDSEQILGNKVLVYLNCCLSGMAYPFG-ALDEENRKRVPLETFRCI----------SSL 696
Cdd:pfam12816   81 VTPLVELLKLIRPLLNEGSdLEEDDEENANKVFPYLSYILTGRQYPTGeGLPEDEASKAKSEIYSFLfsgtsiewppGSG 160

                          170       180       190
                   ....*....|....*....|....*....|.
gi 71984408    697 KEKDGSESDEQYPYLRLLLKYDPQQFLNVVS 727
Cdd:pfam12816  161 TTDPDSDDEPSFPYLRLLLKFDAREFLSVLN 191
ANAPC4_WD40 pfam12894
Anaphase-promoting complex subunit 4 WD40 domain; Apc4 contains an N-terminal propeller-shaped ...
 110-176 1.10e-05

Anaphase-promoting complex subunit 4 WD40 domain; Apc4 contains an N-terminal propeller-shaped WD40 domain.The N-terminus of Afi1 serves to stabilize the union between Apc4 and Apc5, both of which lie towards the bottom-front of the APC,


Pssm-ID: 403945 [Multi-domain]  Cd Length: 91  Bit Score: 44.96  E-value: 1.10e-05
                           10        20        30        40        50        60        70
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 71984408    110 IAVVTSKGQLLLFDLNGnlnqfHR-----GDETMGGASCVTFSLDGKFLAIGLQKGAIKIMTIDN-KLYHMID 176
Cdd:pfam12894   10 IALATEDGELLLHRLNW-----QRvwtlsPDKEDLEVTSLAWRPDGKLLAVGYSDGTVRLLDAENgKIVHHFS 77
 
Name Accession Description Interval E-value
Vps8 pfam12816
Golgi CORVET complex core vacuolar protein 8; Vps8 is one of the Golgi complex components ...
 549-727 2.66e-56

Golgi CORVET complex core vacuolar protein 8; Vps8 is one of the Golgi complex components necessary for vacuolar sorting. Eukaryotic cells contain a highly dynamic endo-membrane system, in which individual organelles keep their identity despite continuous vesicle generation and fusion. Vesicles that bud from a donor membrane are targeted and delivered to each individual organelle, where they release their cargo after fusion with the acceptor membrane. Vps8 is the core component of the endosomal tethering complex CORVET (class C core vacuole/endosome tethering). Vps8 co-operates with Vps21-GTP to mediate endosomal clustering in a reaction that is dependent on Vps3. Vps8 is the only CORVET subunit that is enriched on late endosomes, suggesting that it is a marker for the maturation of late endosomes. Late endosomes form intralumenal vesicles, and the resulting multivesicular bodies fuse with the vacuole to release their cargoes.


Pssm-ID: 463718  Cd Length: 194  Bit Score: 193.54  E-value: 2.66e-56
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 71984408    549 VFLDHLDEYVLDGALIDPPPPLVNEYLQHLAAEGHFSQFQSAVVRFPIHTLDLHTVMSICKQNSIYDGIIYVNNKALNDY 628
Cdd:pfam12816    1 IFLETLEPYILSGRIRSLPPEVVKALVEHYASKGRLSRLEELICHLDPSSLDIDQVVKLCKKHGLYDALIYVWNRALNDY 80

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 71984408    629 ITPLENMLAEMRTFAHRDM-FSDSEQILGNKVLVYLNCCLSGMAYPFG-ALDEENRKRVPLETFRCI----------SSL 696
Cdd:pfam12816   81 VTPLVELLKLIRPLLNEGSdLEEDDEENANKVFPYLSYILTGRQYPTGeGLPEDEASKAKSEIYSFLfsgtsiewppGSG 160

                          170       180       190
                   ....*....|....*....|....*....|.
gi 71984408    697 KEKDGSESDEQYPYLRLLLKYDPQQFLNVVS 727
Cdd:pfam12816  161 TTDPDSDDEPSFPYLRLLLKFDAREFLSVLN 191
ANAPC4_WD40 pfam12894
Anaphase-promoting complex subunit 4 WD40 domain; Apc4 contains an N-terminal propeller-shaped ...
 110-176 1.10e-05

Anaphase-promoting complex subunit 4 WD40 domain; Apc4 contains an N-terminal propeller-shaped WD40 domain.The N-terminus of Afi1 serves to stabilize the union between Apc4 and Apc5, both of which lie towards the bottom-front of the APC,


Pssm-ID: 403945 [Multi-domain]  Cd Length: 91  Bit Score: 44.96  E-value: 1.10e-05
                           10        20        30        40        50        60        70
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 71984408    110 IAVVTSKGQLLLFDLNGnlnqfHR-----GDETMGGASCVTFSLDGKFLAIGLQKGAIKIMTIDN-KLYHMID 176
Cdd:pfam12894   10 IALATEDGELLLHRLNW-----QRvwtlsPDKEDLEVTSLAWRPDGKLLAVGYSDGTVRLLDAENgKIVHHFS 77
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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