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Conserved domains on  [gi|13384672|ref|NP_079586|]
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pre-rRNA 2'-O-ribose RNA methyltransferase FTSJ3 [Mus musculus]

Protein Classification

FTSJ3/SPB1 family RNA methyltransferase( domain architecture ID 10001011)

FTSJ3/SPB1 family RNA methyltransferase is a class I S-adenosyl-L-methionine (SAM)-dependent methyltransferase similar to human pre-rRNA 2'-O-ribose RNA methyltransferase FTSJ3 that is involved in the processing of the 34S pre-rRNA to 18S rRNA and in 40S ribosomal subunit formation

Graphical summary

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List of domain hits

Name Accession Description Interval E-value
Spb1_C pfam07780
Spb1 C-terminal domain; This presumed domain is found at the C-terminus of a family of ...
640-825 1.85e-85

Spb1 C-terminal domain; This presumed domain is found at the C-terminus of a family of FtsJ-like methyltransferases. Members of this family are involved in 60S ribosomal biogenesis, for example Swiss:P25582.


:

Pssm-ID: 462264  Cd Length: 209  Bit Score: 271.02  E-value: 1.85e-85
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 13384672   640 FEVVPIQDPV------------------KYRILDPEGLALGAVIASSKKAKRDLIDNSFNRYAFNEEEGeLPEWFAQEEK 701
Cdd:pfam07780   1 FEIVPAEEDEdkdsdddwdeddekegikKIDILTAEALALAHQLATGKKTKRDLIDDGFNRYAFNDKDG-LPDWFLDDEK 79
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 13384672   702 QHRIRQLPVDKKEVEHYRKRWREINARPIKKVAEAKARKKRRVLKKLEQTKKKAEAVVNTVDISEREKVAQLRSLYKKAG 781
Cdd:pfam07780  80 KHSKPNKPITKEAVAAIKEKLRALNARPIKKVAEAKARKKMRAAKRLEKAKKKAELINEDEDMSEREKAKQIEKLYKKAA 159
                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....
gi 13384672   782 LGKEKRQVTYVVAKKGVGRKVRRPAGVKGHFKVVDSRMKKDQRA 825
Cdd:pfam07780 160 KKKKKPKVKYVVAKGGNKGKKGRPKGVKGRYKMVDPRMKKDLRA 203
RlmE super family cl41855
23S rRNA U2552 (ribose-2'-O)-methylase RlmE/FtsJ [Translation, ribosomal structure and ...
1-200 1.41e-62

23S rRNA U2552 (ribose-2'-O)-methylase RlmE/FtsJ [Translation, ribosomal structure and biogenesis]; 23S rRNA U2552 (ribose-2'-O)-methylase RlmE/FtsJ is part of the Pathway/BioSystem: 23S rRNA modification


The actual alignment was detected with superfamily member COG0293:

Pssm-ID: 440062 [Multi-domain]  Cd Length: 208  Bit Score: 209.54  E-value: 1.41e-62
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 13384672   1 MGKKGKVGKSR------RDKFYHLAKETGYRSRSAFKLIQLNRRFQFLQKARALLDLCAAPGGWLQVAAKFMPVSSLIVG 74
Cdd:COG0293   1 MKMKRSKSSKRwlqrhlNDPYVKRAKKEGYRSRAAYKLLEIDEKDKLIKPGMRVVDLGAAPGGWSQVAAKRVGGKGRVIA 80
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 13384672  75 VDLVPIKPLPNVVTLQEDITTERCRQALRKELKTWKVDVVLNDGAPNVGASWVHDAYSQAHLTLMALRLACDFLARGGCF 154
Cdd:COG0293  81 LDLLPMEPIPGVEFIQGDFREDEVLDQLLEALGGRKVDLVLSDMAPNTSGHKSVDHARSMYLVELALDFARKVLKPGGAF 160
                       170       180       190       200
                ....*....|....*....|....*....|....*....|....*.
gi 13384672 155 ITKVFRSRDYQPLLWIFQQLFHRVQATKPQASRHESAEIFVVCQGF 200
Cdd:COG0293 161 VVKVFQGEGFDELLKELKKLFKKVKHRKPKASRARSSEVYLVAKGF 206
DUF3381 pfam11861
Domain of unknown function (DUF3381); This domain is functionally uncharacterized. This domain ...
231-398 1.20e-39

Domain of unknown function (DUF3381); This domain is functionally uncharacterized. This domain is found in eukaryotes. This presumed domain is typically between 156 to 174 amino acids in length. This domain is found associated with pfam07780, pfam01728.


:

Pssm-ID: 463375 [Multi-domain]  Cd Length: 150  Bit Score: 143.46  E-value: 1.20e-39
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 13384672   231 VTKKKPKAEGYAEGDLTLYHRTSVTDFLRAANPVDFLSKASEISID---DEELAQHPATTEDIRVCCQDIKVLGRKELRS 307
Cdd:pfam11861   1 PEKKKRKREGYEEGDYTLYKEISASEFIKSEDPIDILGSANEITFDdeaDKEILKHPLTTEEIKECCKDLKVLGKKDFKM 80
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 13384672   308 LLNWRTKLRRYVAKKLKEQAKAldislsseeeeegdeeeavaeTKQAPEEEEEREEEQLNRTLAEMKAQEVAELKRKKKK 387
Cdd:pfam11861  81 LLKWRKKLREELGLDKKDEEEE---------------------EEEVVEVEELDEEEQIDKELEELKEEEKAKLKREKKK 139
                         170
                  ....*....|.
gi 13384672   388 LLREQRKQRER 398
Cdd:pfam11861 140 ANERKQKEIER 150
 
Name Accession Description Interval E-value
Spb1_C pfam07780
Spb1 C-terminal domain; This presumed domain is found at the C-terminus of a family of ...
640-825 1.85e-85

Spb1 C-terminal domain; This presumed domain is found at the C-terminus of a family of FtsJ-like methyltransferases. Members of this family are involved in 60S ribosomal biogenesis, for example Swiss:P25582.


Pssm-ID: 462264  Cd Length: 209  Bit Score: 271.02  E-value: 1.85e-85
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 13384672   640 FEVVPIQDPV------------------KYRILDPEGLALGAVIASSKKAKRDLIDNSFNRYAFNEEEGeLPEWFAQEEK 701
Cdd:pfam07780   1 FEIVPAEEDEdkdsdddwdeddekegikKIDILTAEALALAHQLATGKKTKRDLIDDGFNRYAFNDKDG-LPDWFLDDEK 79
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 13384672   702 QHRIRQLPVDKKEVEHYRKRWREINARPIKKVAEAKARKKRRVLKKLEQTKKKAEAVVNTVDISEREKVAQLRSLYKKAG 781
Cdd:pfam07780  80 KHSKPNKPITKEAVAAIKEKLRALNARPIKKVAEAKARKKMRAAKRLEKAKKKAELINEDEDMSEREKAKQIEKLYKKAA 159
                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....
gi 13384672   782 LGKEKRQVTYVVAKKGVGRKVRRPAGVKGHFKVVDSRMKKDQRA 825
Cdd:pfam07780 160 KKKKKPKVKYVVAKGGNKGKKGRPKGVKGRYKMVDPRMKKDLRA 203
RlmE COG0293
23S rRNA U2552 (ribose-2'-O)-methylase RlmE/FtsJ [Translation, ribosomal structure and ...
1-200 1.41e-62

23S rRNA U2552 (ribose-2'-O)-methylase RlmE/FtsJ [Translation, ribosomal structure and biogenesis]; 23S rRNA U2552 (ribose-2'-O)-methylase RlmE/FtsJ is part of the Pathway/BioSystem: 23S rRNA modification


Pssm-ID: 440062 [Multi-domain]  Cd Length: 208  Bit Score: 209.54  E-value: 1.41e-62
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 13384672   1 MGKKGKVGKSR------RDKFYHLAKETGYRSRSAFKLIQLNRRFQFLQKARALLDLCAAPGGWLQVAAKFMPVSSLIVG 74
Cdd:COG0293   1 MKMKRSKSSKRwlqrhlNDPYVKRAKKEGYRSRAAYKLLEIDEKDKLIKPGMRVVDLGAAPGGWSQVAAKRVGGKGRVIA 80
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 13384672  75 VDLVPIKPLPNVVTLQEDITTERCRQALRKELKTWKVDVVLNDGAPNVGASWVHDAYSQAHLTLMALRLACDFLARGGCF 154
Cdd:COG0293  81 LDLLPMEPIPGVEFIQGDFREDEVLDQLLEALGGRKVDLVLSDMAPNTSGHKSVDHARSMYLVELALDFARKVLKPGGAF 160
                       170       180       190       200
                ....*....|....*....|....*....|....*....|....*.
gi 13384672 155 ITKVFRSRDYQPLLWIFQQLFHRVQATKPQASRHESAEIFVVCQGF 200
Cdd:COG0293 161 VVKVFQGEGFDELLKELKKLFKKVKHRKPKASRARSSEVYLVAKGF 206
FtsJ pfam01728
FtsJ-like methyltransferase; This family consists of FtsJ from various bacterial and archaeal ...
24-200 4.87e-57

FtsJ-like methyltransferase; This family consists of FtsJ from various bacterial and archaeal sources FtsJ is a methyltransferase, but actually has no effect on cell division. FtsJ's substrate is the 23S rRNA. The 1.5 A crystal structure of FtsJ in complex with its cofactor S-adenosylmethionine revealed that FtsJ has a methyltransferase fold. This family also includes the N terminus of flaviviral NS5 protein. It has been hypothesized that the N-terminal domain of NS5 is a methyltransferase involved in viral RNA capping.


Pssm-ID: 426399  Cd Length: 179  Bit Score: 193.19  E-value: 4.87e-57
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 13384672    24 YRSRSAFKLIQLNRRFQFLQKARALLDLCAAPGGWLQVAAKFmpVSSLIVGVDLVPI---KPL--PNVVTLQEDITTERC 98
Cdd:pfam01728   1 YRSRAAYKLLEIDEKFGLLKPGKTVLDLGAAPGGWSQVALQR--GAGKVVGVDLGPMqlwKPRndPGVTFIQGDIRDPET 78
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 13384672    99 RQALRKELKtWKVDVVLNDGAPNVGASWVHDAYSQAHLTLMALRLACDFLARGGCFITKVFRSRDYQPLLWIFQQLFHRV 178
Cdd:pfam01728  79 LDLLEELLG-RKVDLVLSDGSPFISGNKVLDHLRSLDLVKAALEVALELLRKGGNFVCKVFQGEDFSELLYLLKLGFEKV 157
                         170       180
                  ....*....|....*....|..
gi 13384672   179 QATKPQASRHESAEIFVVCQGF 200
Cdd:pfam01728 158 GVFKPPASRPESSEEYLVCLGF 179
DUF3381 pfam11861
Domain of unknown function (DUF3381); This domain is functionally uncharacterized. This domain ...
231-398 1.20e-39

Domain of unknown function (DUF3381); This domain is functionally uncharacterized. This domain is found in eukaryotes. This presumed domain is typically between 156 to 174 amino acids in length. This domain is found associated with pfam07780, pfam01728.


Pssm-ID: 463375 [Multi-domain]  Cd Length: 150  Bit Score: 143.46  E-value: 1.20e-39
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 13384672   231 VTKKKPKAEGYAEGDLTLYHRTSVTDFLRAANPVDFLSKASEISID---DEELAQHPATTEDIRVCCQDIKVLGRKELRS 307
Cdd:pfam11861   1 PEKKKRKREGYEEGDYTLYKEISASEFIKSEDPIDILGSANEITFDdeaDKEILKHPLTTEEIKECCKDLKVLGKKDFKM 80
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 13384672   308 LLNWRTKLRRYVAKKLKEQAKAldislsseeeeegdeeeavaeTKQAPEEEEEREEEQLNRTLAEMKAQEVAELKRKKKK 387
Cdd:pfam11861  81 LLKWRKKLREELGLDKKDEEEE---------------------EEEVVEVEELDEEEQIDKELEELKEEEKAKLKREKKK 139
                         170
                  ....*....|.
gi 13384672   388 LLREQRKQRER 398
Cdd:pfam11861 140 ANERKQKEIER 150
rrmJ PRK11188
23S rRNA (uridine(2552)-2'-O)-methyltransferase RlmE;
1-200 5.79e-34

23S rRNA (uridine(2552)-2'-O)-methyltransferase RlmE;


Pssm-ID: 183025  Cd Length: 209  Bit Score: 129.47  E-value: 5.79e-34
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 13384672    1 MGKKGKVGKSR------RDKFYHLAKETGYRSRSAFKLIQLNRRFQFLQKARALLDLCAAPGGWLQVAAKFMPVSSLIVG 74
Cdd:PRK11188   2 TGKKRSASSSRwlqehfSDKYVQQAQKKGLRSRAWFKLDEIQQSDKLFKPGMTVVDLGAAPGGWSQYAVTQIGDKGRVIA 81
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 13384672   75 VDLVPIKPLPNVVTLQEDITTERCRQALRKELKTWKVDVVLNDGAPNVGASWVHDAYSQAHLTLMALRLACDFLARGGCF 154
Cdd:PRK11188  82 CDILPMDPIVGVDFLQGDFRDELVLKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALDMCRDVLAPGGSF 161
                        170       180       190       200
                 ....*....|....*....|....*....|....*....|....*.
gi 13384672  155 ITKVFRSRDYQPLLWIFQQLFHRVQATKPQASRHESAEIFVVCQGF 200
Cdd:PRK11188 162 VVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGR 207
capping_2-OMTase_viral cd20754
viral Cap-0 specific (nucleoside-2'-O-)-methyltransferase; Cap-0 specific (nucleoside-2'-O-) ...
31-189 1.06e-08

viral Cap-0 specific (nucleoside-2'-O-)-methyltransferase; Cap-0 specific (nucleoside-2'-O-)-methyltransferase (2'OMTase) catalyzes the methylation of Cap-0 (m7GpppNp) at the 2'-hydroxyl of the ribose of the first nucleotide, using S-adenosyl-L-methionine (AdoMet) as the methyl donor. This reaction is the fourth and last step in mRNA capping, the creation of the stabilizing five-prime cap (5' cap) on mRNA. Some dsDNA and dsRNA viruses, like the bluetongue virus (BTV), a member of the Reoviridae family, and Vaccinia virus, a member of the Poxviridae family, as well as some ss(+)RNA viruses, like Flaviviridae and Nidovirales, cap their mRNAs and encode their own 2'OMTase. In BTV, all four reactions are catalyzed by a single protein, VP4. In Vaccinia, the activity is located in the processing factor of the poly(A) polymerase, VP39.


Pssm-ID: 467730  Cd Length: 179  Bit Score: 55.53  E-value: 1.06e-08
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 13384672  31 KLIQLNRRFQFLQKARALLDLCAAPGGWLQVAAKfMPVSSLIVGVDLVPIKPL--PNVVTLQEDITTERCRQALRKELKt 108
Cdd:cd20754   3 KLLQLEEYFLYKPEKMRVIYIGCAPGGWLYYLRD-WFEGTLWVGFDPRDTDPLgyNNVITVNKFFDHEHTKLKFLPNKK- 80
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 13384672 109 wkvDVVLND-GAPNVGASWVHDAYSQAHLTLMALRLACdFLARGGCFITKVFRSRDYQPLLWIFQQLFHrvqaTKPQASR 187
Cdd:cd20754  81 ---DLLICDiRSDRSSHVTKEEDTTESFLTLQEGYIAT-KLAKVGSICVKVRAPDLKDDGHFSSGTLFP----QPYAASS 152

                ..
gi 13384672 188 HE 189
Cdd:cd20754 153 SE 154
 
Name Accession Description Interval E-value
Spb1_C pfam07780
Spb1 C-terminal domain; This presumed domain is found at the C-terminus of a family of ...
640-825 1.85e-85

Spb1 C-terminal domain; This presumed domain is found at the C-terminus of a family of FtsJ-like methyltransferases. Members of this family are involved in 60S ribosomal biogenesis, for example Swiss:P25582.


Pssm-ID: 462264  Cd Length: 209  Bit Score: 271.02  E-value: 1.85e-85
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 13384672   640 FEVVPIQDPV------------------KYRILDPEGLALGAVIASSKKAKRDLIDNSFNRYAFNEEEGeLPEWFAQEEK 701
Cdd:pfam07780   1 FEIVPAEEDEdkdsdddwdeddekegikKIDILTAEALALAHQLATGKKTKRDLIDDGFNRYAFNDKDG-LPDWFLDDEK 79
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 13384672   702 QHRIRQLPVDKKEVEHYRKRWREINARPIKKVAEAKARKKRRVLKKLEQTKKKAEAVVNTVDISEREKVAQLRSLYKKAG 781
Cdd:pfam07780  80 KHSKPNKPITKEAVAAIKEKLRALNARPIKKVAEAKARKKMRAAKRLEKAKKKAELINEDEDMSEREKAKQIEKLYKKAA 159
                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....
gi 13384672   782 LGKEKRQVTYVVAKKGVGRKVRRPAGVKGHFKVVDSRMKKDQRA 825
Cdd:pfam07780 160 KKKKKPKVKYVVAKGGNKGKKGRPKGVKGRYKMVDPRMKKDLRA 203
RlmE COG0293
23S rRNA U2552 (ribose-2'-O)-methylase RlmE/FtsJ [Translation, ribosomal structure and ...
1-200 1.41e-62

23S rRNA U2552 (ribose-2'-O)-methylase RlmE/FtsJ [Translation, ribosomal structure and biogenesis]; 23S rRNA U2552 (ribose-2'-O)-methylase RlmE/FtsJ is part of the Pathway/BioSystem: 23S rRNA modification


Pssm-ID: 440062 [Multi-domain]  Cd Length: 208  Bit Score: 209.54  E-value: 1.41e-62
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 13384672   1 MGKKGKVGKSR------RDKFYHLAKETGYRSRSAFKLIQLNRRFQFLQKARALLDLCAAPGGWLQVAAKFMPVSSLIVG 74
Cdd:COG0293   1 MKMKRSKSSKRwlqrhlNDPYVKRAKKEGYRSRAAYKLLEIDEKDKLIKPGMRVVDLGAAPGGWSQVAAKRVGGKGRVIA 80
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 13384672  75 VDLVPIKPLPNVVTLQEDITTERCRQALRKELKTWKVDVVLNDGAPNVGASWVHDAYSQAHLTLMALRLACDFLARGGCF 154
Cdd:COG0293  81 LDLLPMEPIPGVEFIQGDFREDEVLDQLLEALGGRKVDLVLSDMAPNTSGHKSVDHARSMYLVELALDFARKVLKPGGAF 160
                       170       180       190       200
                ....*....|....*....|....*....|....*....|....*.
gi 13384672 155 ITKVFRSRDYQPLLWIFQQLFHRVQATKPQASRHESAEIFVVCQGF 200
Cdd:COG0293 161 VVKVFQGEGFDELLKELKKLFKKVKHRKPKASRARSSEVYLVAKGF 206
FtsJ pfam01728
FtsJ-like methyltransferase; This family consists of FtsJ from various bacterial and archaeal ...
24-200 4.87e-57

FtsJ-like methyltransferase; This family consists of FtsJ from various bacterial and archaeal sources FtsJ is a methyltransferase, but actually has no effect on cell division. FtsJ's substrate is the 23S rRNA. The 1.5 A crystal structure of FtsJ in complex with its cofactor S-adenosylmethionine revealed that FtsJ has a methyltransferase fold. This family also includes the N terminus of flaviviral NS5 protein. It has been hypothesized that the N-terminal domain of NS5 is a methyltransferase involved in viral RNA capping.


Pssm-ID: 426399  Cd Length: 179  Bit Score: 193.19  E-value: 4.87e-57
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 13384672    24 YRSRSAFKLIQLNRRFQFLQKARALLDLCAAPGGWLQVAAKFmpVSSLIVGVDLVPI---KPL--PNVVTLQEDITTERC 98
Cdd:pfam01728   1 YRSRAAYKLLEIDEKFGLLKPGKTVLDLGAAPGGWSQVALQR--GAGKVVGVDLGPMqlwKPRndPGVTFIQGDIRDPET 78
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 13384672    99 RQALRKELKtWKVDVVLNDGAPNVGASWVHDAYSQAHLTLMALRLACDFLARGGCFITKVFRSRDYQPLLWIFQQLFHRV 178
Cdd:pfam01728  79 LDLLEELLG-RKVDLVLSDGSPFISGNKVLDHLRSLDLVKAALEVALELLRKGGNFVCKVFQGEDFSELLYLLKLGFEKV 157
                         170       180
                  ....*....|....*....|..
gi 13384672   179 QATKPQASRHESAEIFVVCQGF 200
Cdd:pfam01728 158 GVFKPPASRPESSEEYLVCLGF 179
DUF3381 pfam11861
Domain of unknown function (DUF3381); This domain is functionally uncharacterized. This domain ...
231-398 1.20e-39

Domain of unknown function (DUF3381); This domain is functionally uncharacterized. This domain is found in eukaryotes. This presumed domain is typically between 156 to 174 amino acids in length. This domain is found associated with pfam07780, pfam01728.


Pssm-ID: 463375 [Multi-domain]  Cd Length: 150  Bit Score: 143.46  E-value: 1.20e-39
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 13384672   231 VTKKKPKAEGYAEGDLTLYHRTSVTDFLRAANPVDFLSKASEISID---DEELAQHPATTEDIRVCCQDIKVLGRKELRS 307
Cdd:pfam11861   1 PEKKKRKREGYEEGDYTLYKEISASEFIKSEDPIDILGSANEITFDdeaDKEILKHPLTTEEIKECCKDLKVLGKKDFKM 80
                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 13384672   308 LLNWRTKLRRYVAKKLKEQAKAldislsseeeeegdeeeavaeTKQAPEEEEEREEEQLNRTLAEMKAQEVAELKRKKKK 387
Cdd:pfam11861  81 LLKWRKKLREELGLDKKDEEEE---------------------EEEVVEVEELDEEEQIDKELEELKEEEKAKLKREKKK 139
                         170
                  ....*....|.
gi 13384672   388 LLREQRKQRER 398
Cdd:pfam11861 140 ANERKQKEIER 150
rrmJ PRK11188
23S rRNA (uridine(2552)-2'-O)-methyltransferase RlmE;
1-200 5.79e-34

23S rRNA (uridine(2552)-2'-O)-methyltransferase RlmE;


Pssm-ID: 183025  Cd Length: 209  Bit Score: 129.47  E-value: 5.79e-34
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 13384672    1 MGKKGKVGKSR------RDKFYHLAKETGYRSRSAFKLIQLNRRFQFLQKARALLDLCAAPGGWLQVAAKFMPVSSLIVG 74
Cdd:PRK11188   2 TGKKRSASSSRwlqehfSDKYVQQAQKKGLRSRAWFKLDEIQQSDKLFKPGMTVVDLGAAPGGWSQYAVTQIGDKGRVIA 81
                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 13384672   75 VDLVPIKPLPNVVTLQEDITTERCRQALRKELKTWKVDVVLNDGAPNVGASWVHDAYSQAHLTLMALRLACDFLARGGCF 154
Cdd:PRK11188  82 CDILPMDPIVGVDFLQGDFRDELVLKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALDMCRDVLAPGGSF 161
                        170       180       190       200
                 ....*....|....*....|....*....|....*....|....*.
gi 13384672  155 ITKVFRSRDYQPLLWIFQQLFHRVQATKPQASRHESAEIFVVCQGF 200
Cdd:PRK11188 162 VVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGR 207
capping_2-OMTase_viral cd20754
viral Cap-0 specific (nucleoside-2'-O-)-methyltransferase; Cap-0 specific (nucleoside-2'-O-) ...
31-189 1.06e-08

viral Cap-0 specific (nucleoside-2'-O-)-methyltransferase; Cap-0 specific (nucleoside-2'-O-)-methyltransferase (2'OMTase) catalyzes the methylation of Cap-0 (m7GpppNp) at the 2'-hydroxyl of the ribose of the first nucleotide, using S-adenosyl-L-methionine (AdoMet) as the methyl donor. This reaction is the fourth and last step in mRNA capping, the creation of the stabilizing five-prime cap (5' cap) on mRNA. Some dsDNA and dsRNA viruses, like the bluetongue virus (BTV), a member of the Reoviridae family, and Vaccinia virus, a member of the Poxviridae family, as well as some ss(+)RNA viruses, like Flaviviridae and Nidovirales, cap their mRNAs and encode their own 2'OMTase. In BTV, all four reactions are catalyzed by a single protein, VP4. In Vaccinia, the activity is located in the processing factor of the poly(A) polymerase, VP39.


Pssm-ID: 467730  Cd Length: 179  Bit Score: 55.53  E-value: 1.06e-08
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 13384672  31 KLIQLNRRFQFLQKARALLDLCAAPGGWLQVAAKfMPVSSLIVGVDLVPIKPL--PNVVTLQEDITTERCRQALRKELKt 108
Cdd:cd20754   3 KLLQLEEYFLYKPEKMRVIYIGCAPGGWLYYLRD-WFEGTLWVGFDPRDTDPLgyNNVITVNKFFDHEHTKLKFLPNKK- 80
                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 13384672 109 wkvDVVLND-GAPNVGASWVHDAYSQAHLTLMALRLACdFLARGGCFITKVFRSRDYQPLLWIFQQLFHrvqaTKPQASR 187
Cdd:cd20754  81 ---DLLICDiRSDRSSHVTKEEDTTESFLTLQEGYIAT-KLAKVGSICVKVRAPDLKDDGHFSSGTLFP----QPYAASS 152

                ..
gi 13384672 188 HE 189
Cdd:cd20754 153 SE 154
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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