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Conserved domains on  [gi|1707289720|gb|TRY35147|]
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heteromeric transposase endonuclease subunit TnsA [Geobacillus sp. LEMMJ02]

Protein Classification

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
TnsA_endonuclease-like cd22362
Transposon Tn7 transposition protein TnsA; TnsA is part of the Tn7 transposon mobile genetic ...
 28-257 2.87e-91

Transposon Tn7 transposition protein TnsA; TnsA is part of the Tn7 transposon mobile genetic element working together with TnsB, TnsC, and TnsD to facilitate insertion of the transposon. TnsA catalyzes cleavage at the transposon 5' ends, and TnsC is the activator of the composite TnsAB transposase. TnsA belongs to a superfamily of nucleases including very short patch repair (Vsr) endonucleases, archaeal Holliday junction resolvases, MutH methyl-directed DNA mismatch-repair endonucleases, and catalytic domains of many restriction endonucleases, such as EcoRI, BamHI, and FokI.


:

Pssm-ID: 411766 [Multi-domain]  Cd Length: 234  Bit Score: 269.58  E-value: 2.87e-91
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1707289720  28 NYKPWIYIQDFPSSGLVTRGKGWKTNRIHHFLSKLERDYFYVLEWNRSVVDIREQFPL-NREDTLFIATNKGIKHPTDPK 106
Cdd:cd22362     1 DYKPWLTVRDVPSRGTSSRVYSPKTGRTHHLLSDLERAAFLLLEWDPNVIDIREQYPLdPLEGTLEIAKELGIKHPRYVG 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1707289720 107 SQIPIVMTTDFLITVTTSAGTKHYARTIKPSKELENKRTIEKLEIERSYWEDRGVDWGIVTEKEMPRNLIENVEWLHSSY 186
Cdd:cd22362    81 TGEPYVMTTDFLVTLRDPGKGRLVAISVKPSDDLKDPRTLEKLEIERRYWERRGIPWRLITESELPKVLARNLEWLRGAR 160

                         170       180       190       200       210       220       230
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1707289720 187 FGIEGLPTSALHTYAQQMKSFIKKYNTSIIEVVTEFDNTFQLENGMGLEILKYLIARREIRVDINQKIHTH 257
Cdd:cd22362   161 RDRLEDSSELEDFLDAFLRALRRNKDLPLSDLLERLDKALGLDLGTALRAFRHLIWTGRLDVDLSQDIELE 231
 
Name Accession Description Interval E-value
TnsA_endonuclease-like cd22362
Transposon Tn7 transposition protein TnsA; TnsA is part of the Tn7 transposon mobile genetic ...
 28-257 2.87e-91

Transposon Tn7 transposition protein TnsA; TnsA is part of the Tn7 transposon mobile genetic element working together with TnsB, TnsC, and TnsD to facilitate insertion of the transposon. TnsA catalyzes cleavage at the transposon 5' ends, and TnsC is the activator of the composite TnsAB transposase. TnsA belongs to a superfamily of nucleases including very short patch repair (Vsr) endonucleases, archaeal Holliday junction resolvases, MutH methyl-directed DNA mismatch-repair endonucleases, and catalytic domains of many restriction endonucleases, such as EcoRI, BamHI, and FokI.


Pssm-ID: 411766 [Multi-domain]  Cd Length: 234  Bit Score: 269.58  E-value: 2.87e-91
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1707289720  28 NYKPWIYIQDFPSSGLVTRGKGWKTNRIHHFLSKLERDYFYVLEWNRSVVDIREQFPL-NREDTLFIATNKGIKHPTDPK 106
Cdd:cd22362     1 DYKPWLTVRDVPSRGTSSRVYSPKTGRTHHLLSDLERAAFLLLEWDPNVIDIREQYPLdPLEGTLEIAKELGIKHPRYVG 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1707289720 107 SQIPIVMTTDFLITVTTSAGTKHYARTIKPSKELENKRTIEKLEIERSYWEDRGVDWGIVTEKEMPRNLIENVEWLHSSY 186
Cdd:cd22362    81 TGEPYVMTTDFLVTLRDPGKGRLVAISVKPSDDLKDPRTLEKLEIERRYWERRGIPWRLITESELPKVLARNLEWLRGAR 160

                         170       180       190       200       210       220       230
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1707289720 187 FGIEGLPTSALHTYAQQMKSFIKKYNTSIIEVVTEFDNTFQLENGMGLEILKYLIARREIRVDINQKIHTH 257
Cdd:cd22362   161 RDRLEDSSELEDFLDAFLRALRRNKDLPLSDLLERLDKALGLDLGTALRAFRHLIWTGRLDVDLSQDIELE 231
Tn7_TnsA-like_N pfam08722
TnsA-like endonuclease N terminal; The Tn7 transposase is composed of proteins TnsA and TnsB. ...
 74-168 7.00e-21

TnsA-like endonuclease N terminal; The Tn7 transposase is composed of proteins TnsA and TnsB. DNA breakage at the 5' end of the transposon is carried out by TnsA, and breakage and joining at the 3' end is carried out by TnsB. The N terminal domain of TnsA is catalytic. This domain is also found in Bacteriophage T4 gp4-like head completion protein whose nuclease activity is required for packaging. This domain contains the catalytic triad of one basic and two acidic residues that form a restriction endonuclease-like active site and conserved between TnsA with gp4 proteins.


Pssm-ID: 462579 [Multi-domain]  Cd Length: 83  Bit Score: 84.19  E-value: 7.00e-21
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1707289720  74 RSVVDIREQFPlnredTLFIATNKGIKHptdpksqipIVMTTDFLITVTTSAGTKHYARTIKPSKELEN--KRTIEKLEI 151
Cdd:pfam08722   1 PDVIDIREQPL-----TIPYASELGIKT---------RVYTPDFLVTYKDGDGKPYEAIEVKPSKDLEDwlLRTLEKLEA 66

                          90
                  ....*....|....*..
gi 1707289720 152 ERSYWEDRGVDWGIVTE 168
Cdd:pfam08722  67 AREYWEERGIPFRIITE 83
 
Name Accession Description Interval E-value
TnsA_endonuclease-like cd22362
Transposon Tn7 transposition protein TnsA; TnsA is part of the Tn7 transposon mobile genetic ...
 28-257 2.87e-91

Transposon Tn7 transposition protein TnsA; TnsA is part of the Tn7 transposon mobile genetic element working together with TnsB, TnsC, and TnsD to facilitate insertion of the transposon. TnsA catalyzes cleavage at the transposon 5' ends, and TnsC is the activator of the composite TnsAB transposase. TnsA belongs to a superfamily of nucleases including very short patch repair (Vsr) endonucleases, archaeal Holliday junction resolvases, MutH methyl-directed DNA mismatch-repair endonucleases, and catalytic domains of many restriction endonucleases, such as EcoRI, BamHI, and FokI.


Pssm-ID: 411766 [Multi-domain]  Cd Length: 234  Bit Score: 269.58  E-value: 2.87e-91
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1707289720  28 NYKPWIYIQDFPSSGLVTRGKGWKTNRIHHFLSKLERDYFYVLEWNRSVVDIREQFPL-NREDTLFIATNKGIKHPTDPK 106
Cdd:cd22362     1 DYKPWLTVRDVPSRGTSSRVYSPKTGRTHHLLSDLERAAFLLLEWDPNVIDIREQYPLdPLEGTLEIAKELGIKHPRYVG 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1707289720 107 SQIPIVMTTDFLITVTTSAGTKHYARTIKPSKELENKRTIEKLEIERSYWEDRGVDWGIVTEKEMPRNLIENVEWLHSSY 186
Cdd:cd22362    81 TGEPYVMTTDFLVTLRDPGKGRLVAISVKPSDDLKDPRTLEKLEIERRYWERRGIPWRLITESELPKVLARNLEWLRGAR 160

                         170       180       190       200       210       220       230
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 1707289720 187 FGIEGLPTSALHTYAQQMKSFIKKYNTSIIEVVTEFDNTFQLENGMGLEILKYLIARREIRVDINQKIHTH 257
Cdd:cd22362   161 RDRLEDSSELEDFLDAFLRALRRNKDLPLSDLLERLDKALGLDLGTALRAFRHLIWTGRLDVDLSQDIELE 231
Tn7_TnsA-like_N pfam08722
TnsA-like endonuclease N terminal; The Tn7 transposase is composed of proteins TnsA and TnsB. ...
 74-168 7.00e-21

TnsA-like endonuclease N terminal; The Tn7 transposase is composed of proteins TnsA and TnsB. DNA breakage at the 5' end of the transposon is carried out by TnsA, and breakage and joining at the 3' end is carried out by TnsB. The N terminal domain of TnsA is catalytic. This domain is also found in Bacteriophage T4 gp4-like head completion protein whose nuclease activity is required for packaging. This domain contains the catalytic triad of one basic and two acidic residues that form a restriction endonuclease-like active site and conserved between TnsA with gp4 proteins.


Pssm-ID: 462579 [Multi-domain]  Cd Length: 83  Bit Score: 84.19  E-value: 7.00e-21
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1707289720  74 RSVVDIREQFPlnredTLFIATNKGIKHptdpksqipIVMTTDFLITVTTSAGTKHYARTIKPSKELEN--KRTIEKLEI 151
Cdd:pfam08722   1 PDVIDIREQPL-----TIPYASELGIKT---------RVYTPDFLVTYKDGDGKPYEAIEVKPSKDLEDwlLRTLEKLEA 66

                          90
                  ....*....|....*..
gi 1707289720 152 ERSYWEDRGVDWGIVTE 168
Cdd:pfam08722  67 AREYWEERGIPFRIITE 83
Tn7_Tnp_TnsA_C pfam08721
TnsA endonuclease C terminal; The Tn7 transposase is composed of proteins TnsA and TnsB. DNA ...
 170-250 9.02e-12

TnsA endonuclease C terminal; The Tn7 transposase is composed of proteins TnsA and TnsB. DNA breakage at the 5' end of the transposon is carried out by TnsA, and breakage and joining at the 3' end is carried out by TnsB. The C terminal domain of TnsA binds DNA.


Pssm-ID: 462578  Cd Length: 83  Bit Score: 59.62  E-value: 9.02e-12
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1707289720 170 EMPRNLIENVEWLHSSYF-GIEGLPTSALHTYAQQMKSFIKK-YNTSIIEVVTEFDNTFQLENGMGLEILKYLIARREIR 247
Cdd:pfam08721   1 EIPKVLAKNIEWLHPYYRiEDSDLSEEELEELAELLLERLREnPDTTLRSLLKELDAEYSLEPGTSLSLIRHLVATKEIL 80


                  ...
gi 1707289720 248 VDI 250
Cdd:pfam08721  81 VDL 83
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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