hypothetical protein; Provisional

                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....
gi 750161981  10 CIGCGACVGGCPVGALSLDDEGKSV-CDEGTCIDCGSCVGTCPV 52
Cdd:PRK13795 583 CVGCGVCVGACPTGAIRIEEGKRKIsVDEEKCIHCGKCTEVCPV 626

EFR1 family ferrodoxin; Members of the family have a C-terminal ferrodoxin domain, with eight conserved Cys residues in two CxxCxxCxxxCP motifs, each of which binds a 4Fe-4S cluster. The N-terminal region resembles flavodoxin domains, with some members of the family recognized by Pfam models PF12724 (Flavodoxin_5) or PF00258 (Flavodoxin_1).

                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....*....
gi 750161981   8 DACIGCGACVGGCPVGALSLDDeGKSVCdEGTCIDCGSCVGTCPVGAIN 56
Cdd:NF038196 185 DKCIGCGICAKVCPVNNIEMED-GKPVW-GHNCTHCLACIHRCPKEAIE 231

4Fe-4S dicluster domain; Superfamily includes proteins containing domains which bind to iron-sulfur clusters. Members include bacterial ferredoxins, various dehydrogenases, and various reductases. Structure of the domain is an alpha-antiparallel beta sandwich. Domain contains two 4Fe4S clusters.

                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|.
gi 750161981  10 CIGCGACVGGCPVGALSLDDEGKS------VCDEGTCIDCGSCVGTCPVGA 54
Cdd:pfam12838  1 CIGCGACVAACPVGAITLDEVGEKkgtktvVIDPERCVGCGACVAVCPTGA 51

hypothetical protein; Provisional

                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....
gi 750161981  10 CIGCGACVGGCPVGALSLDDEGKSV-CDEGTCIDCGSCVGTCPV 52
Cdd:PRK13795 583 CVGCGVCVGACPTGAIRIEEGKRKIsVDEEKCIHCGKCTEVCPV 626

Uncharacterized Fe-S cluster protein [Function unknown];

                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|.
gi 750161981  5 IDKDACIGCGACVGGCPVGALSLDDeGKSVCDEGTCIDCGSCVGTCPVGAI 55
Cdd:COG2768   8 VDEEKCIGCGACVKVCPVGAISIED-GKAVIDPEKCIGCGACIEVCPVGAI 57

Iron only hydrogenase large subunit, C-terminal domain [Energy production and conversion];

                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....
gi 750161981   2 AVNIDKDACIGCGACVGGCPVGALSLDDEGKSVcDEGTCIDCGSCVGTCPVGAI 55
Cdd:COG4624   85 SIIRDKEKCKNCYPCVRACPVKAIKVDDGKAEI-DEEKCISCGQCVAVCPFGAI 137

NAD-dependent dihydropyrimidine dehydrogenase, PreA subunit [Nucleotide transport and metabolism];

                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|....*..
gi 750161981  1 MAVNIDKDACIGCGACVGGCPVGALSLDDEGKS--VCDEGTCIDCGSCVGTCPVGAI 55
Cdd:COG1146   1 MMPVIDTDKCIGCGACVEVCPVDVLELDEEGKKalVINPEECIGCGACELVCPVGAI 57

Uncharacterized polyferredoxin-like protein; This family contains uncharacterized polyferredoxin protein similar to Methanobacterium thermoautotrophicum MvhB. The mvhB is a gene of the methylviologen-reducing hydrogenase operon. It is predicted to contain 12 [4Fe-4S] clusters, and was therefore suggested to be a polyferredoxin. As a subfamily of the beta subunit of the DMSO Reductase (DMSOR) family, it is predicted to function as electron carrier in the reducing reaction.

                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|.
gi 750161981   5 IDKDACIGCGACVGGCPVGALSLDDEGKSVCDEGTCIDCGSCVGTCPVGAI 55
Cdd:cd10549   75 IDEEKCIGCGLCVKVCPVDAITLEDELEIVIDKEKCIGCGICAEVCPVNAI 125

Formate hydrogenlyase subunit 6/NADH:ubiquinone oxidoreductase 23 kD subunit (chain I) [Energy production and conversion]; Formate hydrogenlyase subunit 6/NADH:ubiquinone oxidoreductase 23 kD subunit (chain I) is part of the Pathway/BioSystem: NADH dehydrogenase

                        10        20        30        40        50
                ....*....|....*....|....*....|....*....|....*....|..
gi 750161981  7 KDACIGCGACVGGCPVGALSLDDEGKSVC---DEGTCIDCGSCVGTCPVGAI 55
Cdd:COG1143   1 EDKCIGCGLCVRVCPVDAITIEDGEPGKVyviDPDKCIGCGLCVEVCPTGAI 52

Uncharacterized polyferredoxin-like protein; This family contains uncharacterized polyferredoxin protein similar to Methanobacterium thermoautotrophicum MvhB. The mvhB is a gene of the methylviologen-reducing hydrogenase operon. It is predicted to contain 12 [4Fe-4S] clusters, and was therefore suggested to be a polyferredoxin. As a subfamily of the beta subunit of the DMSO Reductase (DMSOR) family, it is predicted to function as electron carrier in the reducing reaction.

                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*
gi 750161981   5 IDKDACIGCGACVGGCPVGALSLDDEGKS----VCDEGTCIDCGSCVGTCPVGAI 55
Cdd:cd10549    3 YDPEKCIGCGICVKACPTDAIELGPNGAIargpEIDEDKCVFCGACVEVCPTGAI 57

formate hydrogenlyase complex iron-sulfur subunit; Provisional

                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|...
gi 750161981   8 DACIGCGACVGGCPVGALSLD-DEGKSVC----DEGTCIDCGSCVGTCPVGAI 55
Cdd:PRK12387  38 QQCIGCAACVNACPSNALTVEtDLATGELawefNLGRCIFCGRCEEVCPTAAI 90

pyruvate ferredoxin oxidoreductase subunit delta; Reviewed

                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|
gi 750161981   6 DKDACIGCGACVGGCPVGALSLDDEGKSVCDEGTCIDCGSCVGTCPVGAI 55
Cdd:PRK09624  49 NRDKCVRCYLCYIYCPEPAIYLDEEGYPVFDYDYCKGCGICANECPTKAI 98

NADH-quinone oxidoreductase, chain I; This model represents the I subunit (one of 14: A->N) of the NADH-quinone oxidoreductase complex I which generally couples NADH and ubiquinone oxidation/reduction in bacteria and mammalian mitochondria, but may act on NADPH and/or plastoquinone in cyanobacteria and plant chloroplasts. This model excludes "I" subunits from the closely related F420H2 dehydrogenase and formate hydrogenlyase complexes. [Energy metabolism, Electron transport]

                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*...
gi 750161981    7 KDACIGCGACVGGCPVGALSLDDE---------GKSVCDEGTCIDCGSCVGTCPVGAI 55
Cdd:TIGR01971  42 EEKCIGCTLCAAVCPADAIRVVPAegedgkrrlKFYEINFGRCIFCGLCEEACPTDAI 99

Na+-translocating ferredoxin:NAD+ oxidoreductase RNF, RnfB subunit [Energy production and conversion]; Na+-translocating ferredoxin:NAD+ oxidoreductase RNF, RnfB subunit is part of the Pathway/BioSystem: Na+-translocating Fd:NADH oxidoreductase

                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|
gi 750161981   6 DKDACIGCGACVGGCPVGALSLDDEGKSVCDEGTCIDCGSCVGTCPVGAI 55
Cdd:COG2878  135 CEYGCIGCGDCIKACPFDAIVGAAKGMHTVDEDKCTGCGLCVEACPVDCI 184

ribosome biogenesis/translation initiation ATPase RLI;

                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|...
gi 750161981   2 AVnIDKDACIG--CG-ACVGGCPVG-----ALSLDDE-GKSVCDEGTCIDCGSCVGTCPVGAI 55
Cdd:PRK13409   5 AV-VDYDRCQPkkCNyECIKYCPVVrtgeeTIEIDEDdGKPVISEELCIGCGICVKKCPFDAI 66

Beta subunit of the DMSO Reductase (DMSOR) family; This family consists of the small beta iron-sulfur (FeS) subunit of the DMSO Reductase (DMSOR) family. Members of this family also contain a large, periplasmic molybdenum-containing alpha subunit and may have a small gamma subunit as well. Examples of heterodimeric members with alpha and beta subunits include arsenite oxidase, and tungsten-containing formate dehydrogenase (FDH-T) while heterotrimeric members containing alpha, beta, and gamma subunits include formate dehydrogenase-N (FDH-N), and nitrate reductase (NarGHI). The beta subunit contains four Fe4/S4 and/or Fe3/S4 clusters which transfer the electrons from the alpha subunit to a hydrophobic integral membrane protein, presumably a cytochrome containing two b-type heme groups. The reducing equivalents are then transferred to menaquinone, which finally reduces the electron-accepting enzyme system.

                         10        20        30        40
                 ....*....|....*....|....*....|....*....|..
gi 750161981  15 ACVGGCPVGALSLDDEGKSVCDEGTCIDCGSCVGTCPVGAIN 56
Cdd:cd04410   57 PCVKACPTGAIYKDEDGIVLIDEDKCIGCGSCVEACPYGAIV 98

NADH-quinone oxidoreductase subunit NuoI;

                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*
gi 750161981  10 CIGCGACVGGCPVGALSLD----DEGKSVCDE-----GTCIDCGSCVGTCPVGAI 55
Cdd:PRK05888  60 CIACKLCAAICPADAITIEaaerEDGRRRTTRydinfGRCIFCGFCEEACPTDAI 114

ferredoxin-like protein;

                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....
gi 750161981   2 AVNIDKDACIGCGACVGGCPVGALSLDDEGKSvcdEGTCIDCGSCVGTCPVGAI 55
Cdd:PRK09898 148 CITVDHKRCIGCSACTTACPWMMATVNTESKK---SSKCVLCGECANACPTGAL 198

uncharacterized subfamily of DMSO Reductase beta subunit family; This family consists of the small beta iron-sulfur (FeS) subunit of the DMSO Reductase (DMSOR) family. Members of this family also contain a large, periplasmic molybdenum-containing alpha subunit and may have a small gamma subunit as well. Examples of heterodimeric members with alpha and beta subunits include arsenite oxidase, and tungsten-containing formate dehydrogenase (FDH-T) while heterotrimeric members containing alpha, beta, and gamma subunits include formate dehydrogenase-N (FDH-N), and nitrate reductase (NarGHI). The beta subunit contains four Fe4/S4 and/or Fe3/S4 clusters which transfer the electrons from the alpha subunit to a hydrophobic integral membrane protein, presumably a cytochrome containing two b-type heme groups. The reducing equivalents are then transferred to menaquinone, which finally reduces the electron-accepting enzyme system.

                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|...
gi 750161981   3 VNIDKDACIGCGACVGGCPVGALSLDDEgksVCDEGTCIDCGSCVGTCPVGAI 55
Cdd:cd16372   72 VMINKKLCVGCLMCVGFCPEGAMFKHED---YPEPFKCIACGICVKACPTGAL 121

DNA-directed RNA polymerase subunit D; Provisional

                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....
gi 750161981   3 VNIDKDaCIGCGACVGGCPVGALSLDDEGKSVCDEGTCIDCGSCVGTCPVGAIN 56
Cdd:PRK00783 165 IEVSED-CDECEKCVEACPRGVLELKEGKLVVTDLLNCSLCKLCERACPGKAIR 217

NAD-dependent dihydropyrimidine dehydrogenase subunit PreA;

                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*...
gi 750161981   4 NIDKDACIGCGACVGGCPVG---ALSLDDEGKS--VCDEGTCIDCGSCVGTCPV-GAI 55
Cdd:PRK08318 338 RIDQDKCIGCGRCYIACEDTshqAIEWDEDGTRtpEVIEEECVGCNLCAHVCPVeGCI 395

D subunit of Archaeal RNA polymerase; The D subunit of archaeal RNA polymerase (RNAP) is involved in the assembly of RNAP subunits. RNAP is a large multi-subunit complex responsible for the synthesis of RNA. It is the principal enzyme of the transcription process, and is a final target in many regulatory pathways that control gene expression in all living cells. A single distinct RNAP complex is found in archaea, which may be responsible for the synthesis of all RNAs. The archaeal RNAP harbors homologues of all eukaryotic RNAP II subunits with two exceptions (RPB8 and RPB9). The 12 archaeal subunits are designated by letters and can be divided into three functional groups that are engaged in: (I) catalysis (A'/A", B'/B" or B); (II) assembly (L, N, D and P); and (III) auxiliary functions (F, E, H and K). The D subunit is equivalent to the RPB3 subunit of eukaryotic RNAP II. It contains two subdomains: one subdomain is similar the eukaryotic Rpb11/AC19/archaeal L subunit which is involved in dimerization, and the other is an inserted beta sheet subdomain. The assembly of the two largest archaeal RNAP subunits that provide most of the enzyme's catalytic functions depends on the presence of the archaeal D/L heterodimer.

                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....
gi 750161981   3 VNIDKDaCIGCGACVGGCPVGALSLDDEGKSVCDEGTCIDCGSCVGTCPVGAIN 56
Cdd:cd07030  165 IEIDED-CDGCGKCVEECPRGVLELEEGKVVVEDLEDCSLCKLCERACDAGAIR 217

uncharacterized subfamily of DMSO Reductase beta subunit family; This family consists of the small beta iron-sulfur (FeS) subunit of the DMSO Reductase (DMSOR) family. Members of this family also contain a large, periplasmic molybdenum-containing alpha subunit and may have a small gamma subunit as well. Examples of heterodimeric members with alpha and beta subunits include arsenite oxidase, and tungsten-containing formate dehydrogenase (FDH-T) while heterotrimeric members containing alpha, beta, and gamma subunits include formate dehydrogenase-N (FDH-N), and nitrate reductase (NarGHI). The beta subunit contains four Fe4/S4 and/or Fe3/S4 clusters which transfer the electrons from the alpha subunit to a hydrophobic integral membrane protein, presumably a cytochrome containing two b-type heme groups. The reducing equivalents are then transferred to menaquinone, which finally reduces the electron-accepting enzyme system.

                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|...
gi 750161981   3 VNIDKDACIGCGACVGGCPVGALSLDDEGKSVCdegTCIDCGSCVGTCPVGAI 55
Cdd:cd16370   78 VVLDKEKCIGCGNCVKACIVGAIFWDEETNKPI---ICIHCGYCARYCPHDVL 127

hydrogenase 4 subunit H; Validated

                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*
gi 750161981   6 DKDACIGCGACVGGCPVGALSLDDEGKSVCDE-----GTCIDCGSCVGTCPVGAI 55
Cdd:PRK08222  36 MPSQCIACGACTCACPANALTIQTDDQQNSRTwqlylGRCIYCGRCEEVCPTRAI 90

uncharacterized subfamily of beta subunit of formate dehydrogenase; This subfamily includes the beta-subunit of formate dehydrogenases that are as yet uncharacterized. Members of the DMSO reductase family include formate dehydrogenase N and O (FDH-N, FDH-O) and tungsten-containing formate dehydrogenase (W-FDH) and other similar proteins. FDH-N, a major component of nitrate respiration of Escherichia coli, is involved in the major anaerobic respiratory pathway in the presence of nitrate, catalyzing the oxidation of formate to carbon dioxide at the expense of nitrate reduction to nitrite. It forms a heterotrimer; the alpha-subunit (FDH-G) is the catalytic site of formate oxidation and membrane-associated, incorporating a selenocysteine (SeCys) residue and a [4Fe/4S] cluster in addition to two bis-MGD cofactors, the beta subunit (FDH-H) contains four [4Fe/4S] clusters which transfer the electrons from the alpha subunit to the gamma-subunit (FDH-I), a hydrophobic integral membrane protein, presumably a cytochrome containing two b-type heme groups. W-FDH contains a tungsten instead of molybdenum at the catalytic center. This enzyme seems to be exclusively found in organisms such as hyperthermophilic archaea that live in extreme environments. It is a heterodimer of a large and a small subunit; the large subunit harbors the W site and one [4Fe-4S] center and the small subunit, containing three [4Fe-4S] clusters, functions to transfer electrons.

                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|
gi 750161981   2 AVNIDKDACIGCGACVGGCPVGALSLDDEGKSVCDEGTCID------CGSCVGTCPVGAI 55
Cdd:cd10562   94 AVVVDEDKCIGCGYCVAACPFDVPRYDETTNKITKCTLCFDriengmQPACVKTCPTGAL 153

Fe-S cluster domain-containing protein;

                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....*...
gi 750161981   9 ACIGCGACVGGCPVGALSLDDeGKSVCDEGTCIDCGSCVGTCPVGAIN 56
Cdd:PRK07118 214 GCIGCGKCVKACPAGAITMEN-NLAVIDQEKCTSCGKCVEKCPTKAIR 260

Na+-translocating ferredoxin:NAD+ oxidoreductase RNF, RnfC subunit [Energy production and conversion]; Na+-translocating ferredoxin:NAD+ oxidoreductase RNF, RnfC subunit is part of the Pathway/BioSystem: Na+-translocating Fd:NADH oxidoreductase

                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|..
gi 750161981   9 ACIGCGACVGGCPVG-------ALSLDDEGKSVCDEG--TCIDCGSCVGTCP 51
Cdd:COG4656  365 PCIRCGRCVDACPMGllpqqlyWYARAGDFDKAEEYNlmDCIECGCCSYVCP 416

Fe-S cluster domain-containing protein;

                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....*..
gi 750161981   9 ACIGCGACVGGCPVGALSLdDEGKSVCDEGTCIDCGSCVGTCPVGAI 55
Cdd:PRK07118 140 GCLGLGSCVAACPFDAIHI-ENGLPVVDEDKCTGCGACVKACPRNVI 185

The beta FeS subunit of formate dehydrogenase-N (FDH-N); This subfamily contains beta FeS subunit of formate dehydrogenase-N (FDH-N), a member of the DMSO reductase family. FDH-N is involved in the major anaerobic respiratory pathway in the presence of nitrate, catalyzing the oxidation of formate to carbon dioxide at the expense of nitrate reduction to nitrite. Thus, FDH-N is a major component of nitrate respiration of Escherichia coli. This integral membrane enzyme forms a heterotrimer; the alpha-subunit (FDH-G) is the catalytic site of formate oxidation and membrane-associated, incorporating a selenocysteine (SeCys) residue and a [4Fe/4S] cluster in addition to two bis-MGD cofactors, the beta subunit (FDH-H) contains four [4Fe/4S] clusters which transfer the electrons from the alpha subunit to the gamma-subunit (FDH-I), a hydrophobic integral membrane protein, presumably a cytochrome containing two b-type heme groups.

                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*....
gi 750161981   3 VNIDKDACIGCGACVGGCPVGALSLDDEGKSVCDEGTCIDCGS------CVGTCPVGAI 55
Cdd:cd10558   96 VDFQSDKCIGCGYCIKGCPFDIPRISKDDNKMYKCTLCSDRVSvglepaCVKTCPTGAL 154

uncharacterized subfamily of DMSO Reductase beta subunit family; This family consists of the small beta iron-sulfur (FeS) subunit of the DMSO Reductase (DMSOR) family. Members of this family also contain a large, periplasmic molybdenum-containing alpha subunit and may have a small gamma subunit as well. Examples of heterodimeric members with alpha and beta subunits include arsenite oxidase, and tungsten-containing formate dehydrogenase (FDH-T) while heterotrimeric members containing alpha, beta, and gamma subunits include formate dehydrogenase-N (FDH-N), and nitrate reductase (NarGHI). The beta subunit contains four Fe4/S4 and/or Fe3/S4 clusters which transfer the electrons from the alpha subunit to a hydrophobic integral membrane protein, presumably a cytochrome containing two b-type heme groups. The reducing equivalents are then transferred to menaquinone, which finally reduces the electron-accepting enzyme system.

                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 750161981   3 VNIDKDACIGCGACVGGCPVGALSLDDEGKSV--CDegTCIDCGS------CVGTCPVGAI 55
Cdd:cd16371   79 VVVDQDKCIGCGYCVWACPYGAPQYNPETGKMdkCD--MCVDRLDegekpaCVAACPTRAL 137

electron transport complex subunit RsxB;

                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|.
gi 750161981   5 IDKDACIGCGACVGGCPVGALSLDDEGKSVCDEGTCIDCGSCVGTCPVGAI 55
Cdd:PRK06991  82 IDEQLCIGCTLCMQACPVDAIVGAPKQMHTVLADLCTGCDLCVPPCPVDCI 132

F420H2 dehydrogenase subunit F; Provisional

                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*....
gi 750161981   5 IDKDACIGCGACVGGCPVGALSLDDEGK-------SVCDEG----TCIDCGSCVGTCPV 52
Cdd:PRK09326   9 IEYDVCTACGACEAVCPIGAITVDKKAEirdpndlELYEKGaapnVCEGCLTCSRICPV 67

putative glutamate synthase (NADPH) small subunit; Provisional

                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....*..
gi 750161981  10 CIGCGACVGGCPVGALSLDDEGKSV-CDEGTCIDCGSCVGTCPVGAI 55
Cdd:PRK12771 512 CFECDNCYGACPQDAIIKLGPGRRYhFDYDKCTGCHICADVCPCGAI 558

NapF, iron-sulfur subunit of periplasmic nitrate reductase; This family contains NapF protein, the iron-sulfur subunit of periplasmic nitrate reductase. The periplasmic nitrate reductase NapABC of Escherichia coli likely functions during anaerobic growth in low-nitrate environments; napF operon expression is activated by cyclic AMP receptor protein (Crp). NapF is a subfamily of the beta subunit of DMSO reductase (DMSOR) family. DMSOR family members have a large, periplasmic molybdenum-containing alpha subunit as well as a small beta FeS subunit, and may also have a small gamma subunit. The beta subunit contains four Fe4/S4 and/or Fe3/S4 clusters which transfer the electrons from the alpha subunit to a hydrophobic integral membrane protein, presumably a cytochrome containing two b-type heme groups. The reducing equivalents are then transferred to menaquinone, which finally reduces the electron-accepting enzyme system.

                         10        20
                 ....*....|....*....|...
gi 750161981   5 IDKDACIGCGACVGGCPVGALSL 27
Cdd:cd10564  114 LDADACTGCGACVSVCPVGAITL 136

putative oxidoreductase Fe-S binding subunit; Reviewed

                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....*....
gi 750161981   9 ACIGC--GACVGGCPVGALSLDDEGKSVcDEGTCIDCGSCVGTCPVGAI 55
Cdd:PRK12809  55 ACHHCnnAPCVTACPVNALTFQSDSVQL-DEQKCIGCKRCAIACPFGVV 102

putative oxidoreductase Fe-S binding subunit; Reviewed

                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*.
gi 750161981   2 AVNIDKDACIGCGACVGGCPVGALSLDDEGKSVCDEGTCIDCG--SCVGTCPVGAI 55
Cdd:PRK12809  79 SVQLDEQKCIGCKRCAIACPFGVVEMVDTIAQKCDLCNQRSSGtqACIEVCPTQAL 134

uncharacterized subfamily of DMSO Reductase beta subunit family; This family consists of the small beta iron-sulfur (FeS) subunit of the DMSO Reductase (DMSOR) family. Members of this family also contain a large, periplasmic molybdenum-containing alpha subunit and may have a small gamma subunit as well. Examples of heterodimeric members with alpha and beta subunits include arsenite oxidase, and tungsten-containing formate dehydrogenase (FDH-T) while heterotrimeric members containing alpha, beta, and gamma subunits include formate dehydrogenase-N (FDH-N), and nitrate reductase (NarGHI). The beta subunit contains four Fe4/S4 and/or Fe3/S4 clusters which transfer the electrons from the alpha subunit to a hydrophobic integral membrane protein, presumably a cytochrome containing two b-type heme groups. The reducing equivalents are then transferred to menaquinone, which finally reduces the electron-accepting enzyme system.

                         10        20        30        40
                 ....*....|....*....|....*....|....*....|
gi 750161981  16 CVGGCPVGALSLDDEGKSVCDEGTCIDCGSCVGTCPVGAI 55
Cdd:cd16374   51 CMEVCPTGAIYRDEDGAVLVDPDKCIGCGMCAMACPFGVP 90

CooF, iron-sulfur subunit of carbon monoxide dehydrogenase; This family includes CooF, the iron-sulfur subunit of carbon monoxide dehydrogenase (CODH), found in anaerobic bacteria and archaea. Carbon monoxide dehydrogenase is a key enzyme for carbon monoxide (CO) metabolism, where CooF is the proposed mediator of electron transfer between CODH and the CO-induced hydrogenase, catalyzing the reaction that uses CO as a single carbon and energy source, and producing only H2 and CO2. The ion-sulfur subunit contains four Fe4/S4 and/or Fe3/S4 clusters which transfer the electrons in the protein complex during reaction.

                         10        20        30        40
                 ....*....|....*....|....*....|....*....|..
gi 750161981  15 ACVGGCPVGALSLDDE-GKSVCDEGTCIDCGSCVGTCPVGAI 55
Cdd:cd10563   64 PCVKACMSGAMHKDPEtGIVIHDEEKCVGCWMCVMVCPYGAI 105

ferredoxin; Provisional

                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 750161981   4 NIDKDACIGCGACVGGCPVGALSLDD-------EGKS-----VCDEGTCIDCGSCVGTCPVGAI 55
Cdd:PRK06273  45 KVFEELCIGCGGCANVCPTKAIEMIPvepvkitEGYVktkipKIDYEKCVYCLYCHDFCPVFAL 108

Uncharacterized Fe-S cluster protein [Function unknown];

                        10        20
                ....*....|....*....|....*.
gi 750161981  5 IDKDACIGCGACVGGCPVGALSLDDE 30
Cdd:COG2768  37 IDPEKCIGCGACIEVCPVGAIKIEWE 62

beta subunit of formate dehydrogenase O (FDH-O) and similar proteins; This subfamily includes beta subunit of formate dehydrogenase family O (FDH-O), which is highly homologous to formate dehydrogenase N (FDH-N), a member of the DMSO reductase family. In E. coli three formate dehydrogenases are synthesized that are capable of oxidizing formate; Fdh-H, couples formate disproportionation to hydrogen and CO2, and is part of the cytoplasmically oriented formate hydrogenlyase complex, while FDH-N and FDH-O indicate their respective induction after growth with nitrate and oxygen. Little is known about FDH-O, although it shows formate oxidase activity during aerobic growth and is also synthesized during nitrate respiration, similar to FDH-N.

                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|
gi 750161981   8 DACIGC--GACVGGCPVGALSLDDEGKSVCDEGTCIDCGSCVGTCPVGAI 55
Cdd:cd10560   76 DVCKHCtdAGCLEACPTGAIFRTEFGTVYIQPDICNGCGYCVAACPFGVI 125

Heterodisulfide reductase, subunit A (polyferredoxin) [Energy production and conversion];

                         10        20
                 ....*....|....*....|....*
gi 750161981   5 IDKDACIGCGACVGGCPVGALSLDD 29
Cdd:COG1148  523 VNPALCKGCGTCAAACPSGAISLKG 547

the FeS subunit of hydrogenase 2; This subfamily includes the beta-subunit of hydrogenase 2 (Hyd-2), an enzyme that catalyzes the reversible oxidation of H2 to protons and electrons. Hyd-2 is membrane-associated and forms an unusual heterotetrameric [NiFe]-hydrogenase in that it lacks the typical cytochrome b membrane anchor subunit that transfers electrons to the quinone pool. The electron transfer subunit of Hyd-2 (HybA) which is predicted to contain four iron-sulfur clusters, is essential for electron transfer from Hyd-2 to menaquinone/demethylmenaquinone (MQ/DMQ) to couple hydrogen oxidation to fumarate reduction.

                         10        20        30
                 ....*....|....*....|....*....|....*..
gi 750161981  15 ACVGGCPVGALSLDDEGKSVCDEGTCIDCGSCVGTCP 51
Cdd:cd10561   76 ACVSACPVGALRKTPEGPVTYDEDKCIGCRYCMVACP 112

beta subunit of formate dehydrogenase O (FDH-O) and similar proteins; This subfamily includes beta subunit of formate dehydrogenase family O (FDH-O), which is highly homologous to formate dehydrogenase N (FDH-N), a member of the DMSO reductase family. In E. coli three formate dehydrogenases are synthesized that are capable of oxidizing formate; Fdh-H, couples formate disproportionation to hydrogen and CO2, and is part of the cytoplasmically oriented formate hydrogenlyase complex, while FDH-N and FDH-O indicate their respective induction after growth with nitrate and oxygen. Little is known about FDH-O, although it shows formate oxidase activity during aerobic growth and is also synthesized during nitrate respiration, similar to FDH-N.

                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 750161981   2 AVNIDKDACIGCGACVGGCPVG--ALSLDDEGKSVCDEgtCID------CGSCVGTCPVGAI 55
Cdd:cd10560  102 TVYIQPDICNGCGYCVAACPFGviDRNEETGRAHKCTL--CYDrlkdglEPACAKACPTGSI 161

polysulfide reductase beta (PsrB) subunit; This family includes the beta subunit of bacterial polysulfide reductase (PsrABC), an integral membrane-bound enzyme responsible for quinone-coupled reduction of polysulfides, a process important in extreme environments such as deep-sea vents and hot springs. Polysulfide reductase contains three subunits: a catalytic subunit PsrA, an electron transfer PsrB subunit and the hydrophobic transmembrane PsrC subunit. PsrB belongs to the DMSO reductase superfamily that contains [4Fe-4S] clusters which transfer the electrons from the A subunit to the hydrophobic integral membrane C subunit via the B subunit. In Shewanella oneidensis, which has highly diverse anaerobic respiratory pathways, PsrABC is responsible for H2S generation as well as its regulation via respiration of sulfur species. PsrB transfers electrons from PsrC (serving as quinol oxidase) to the catalytic subunit PsrA for reduction of corresponding electron acceptors. It has been shown that T. thermophilus polysulfide reductase could be a key energy-conserving enzyme of the respiratory chain, using polysulfide as the terminal electron acceptor and pumping protons across the membrane.

                         10        20        30        40
                 ....*....|....*....|....*....|....*....|.
gi 750161981  15 ACVGGCPVGALSLDDEGKSVCDEGTCIDCGSCVGTCPVGAI 55
Cdd:cd10551   60 PCVKVCPTGATYKREDGIVLVDYDKCIGCRYCMAACPYGAR 100

Succinate dehydrogenase/fumarate reductase, Fe-S protein subunit [Energy production and conversion]; Succinate dehydrogenase/fumarate reductase, Fe-S protein subunit is part of the Pathway/BioSystem: TCA cycle

                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 750161981   9 ACIGCGACVGGCPVGALSLD------------------DEGKS-----VCDE---GTCIDCGSCVGTCPVG 53
Cdd:COG0479  143 ECILCGACVAACPNVWANPDflgpaalaqayrfaldprDEETEerleaLEDEegvWRCTTCGNCTEVCPKG 213