N-terminal domain of Kruppel-like factor 5; Kruppel-like factor 5 (KLF5; also known as also known as Krueppel-like factor 5; intestinal enriched Kruppel-like factor/IKLF; basic transcription element binding protein 2/BTEB2) a protein that in humans is encoded by the KLF5 gene. KLF5 is involved in numerous functions in eukaryotic cells, such as proliferation, migration, and differentiation. The loss of KLF5 expression is associated with tumors of the breast, cervix, endometrium, ovary, and prostate. KLF5 mediates the expression of several genes essential for proper cardiac structure and function, and plays a role in familial dilated cardiomyopathy. It functions as a transcriptional activator. KLF5 exhibits both transcriptional activation activity as well as trans-activating function. It belongs to a family of proteins called the Specificity Protein (SP)/KLF family, characterized by a C-terminal DNA-binding domain of 81 amino acids consisting of three Kruppel-like C2H2 zinc fingers. These factors bind to a loose consensus motif, namely NNRCRCCYY (where N is any nucleotide; R is A/G, and Y is C/T), such as the recurring motifs in GC and GT boxes (5'-GGGGCGGGG-3' and 5-GGTGTGGGG-3') that are present in promoters and more distal regulatory elements of mammalian genes. Members of the KLF family can act as activators or repressors of transcription depending on cell and promoter context. KLFs regulate various cellular functions, such as proliferation, differentiation, and apoptosis, as well as the development and homeostasis of several types of tissue. In addition to the C-terminal DNA-binding domain, each KLF also has a unique N-terminal activation/repression domain that confers specificity and allows it to bind specifically to a certain partner, leading to distinct activities in vivo. This model represents the N-terminal domain of KLF5.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 528468400   3 ATLLTMSAALAPGQDDFLSLLKPSLSDGFQEDSSLfCFEHKNPDSERGLSNDYSQAKLEMDKYLSPQP--VVLPDLKKSR 80
Cdd:cd21579    1 AAGLRMSAGLAPGEEAVFTQLKPVLMSGGDGEESL-LFGDKPAVRGRSSPHDYNQTKSEMDKYLSPQPppPPAPEDKKYR 79

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 528468400  81 RESVSTMDQYFGDDHGSPYSINMNVYLPDIAYLRTGLCRAARHSVSnQVKTEPVtapfsHPECPVSSSGPAPSALPDFTS 160
Cdd:cd21579   80 RESASVVDEFFSEDKGSPYSVNMNVFLPDVTYLRTGLCRPVRPIKT-EPKHEPP-----PPTSFCSSSNGTTQALPEFTS 153

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 528468400 161 IFNSNSTSGSgmdvvddsaGVFVKQEMQGFDIPQDGPLFQLLNSELDVpdptdphahsqmptfhnlhlaaqqtaaksycg 240
Cdd:cd21579  154 VFSAPQSAVN---------NVFIKQEMPSFDDQQQGPLFQLLNSDLDQ-------------------------------- 192

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 528468400 241 maahgyplshghfvhpqdqahsQLKMTYLPPSPPTSEPGSPDRQKELLHNLTPPPSYAATIASKMAGHAPSHPTP-ASAR 319
Cdd:cd21579  193 ----------------------QQQPTYLPPSPPNSEPGSPDRQKELLHNLSPPPSYAASIASKLAGQTPGLPPPgVGPL 250

                        330       340
                 ....*....|....*....|...
gi 528468400 320 PPTTTGSMPVRYNRRTNPDLEKR 342
Cdd:cd21579  251 SPGQAQSAPVRYNRRNNPDLEKR 273

FOG: Zn-finger [General function prediction only];

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 528468400 322 TTTGSMPVRYNRRTNPDLEKRRIHHCDFpgCKKVYTKSSHLKAHLRTHTGEKPYRCTWEGCDWRFARSDELTRHFRKHTG 401
Cdd:COG5048   11 SNNSVLSSTPKSTLKSLSNAPRPDSCPN--CTDSFSRLEHLTRHIRSHTGEKPSQCSYSGCDKSFSRPLELSRHLRTHHN 88

                 ....*..
gi 528468400 402 AKPFQCA 408
Cdd:COG5048   89 NPSDLNS 95

N-terminal domain of Kruppel-like factor 5; Kruppel-like factor 5 (KLF5; also known as also known as Krueppel-like factor 5; intestinal enriched Kruppel-like factor/IKLF; basic transcription element binding protein 2/BTEB2) a protein that in humans is encoded by the KLF5 gene. KLF5 is involved in numerous functions in eukaryotic cells, such as proliferation, migration, and differentiation. The loss of KLF5 expression is associated with tumors of the breast, cervix, endometrium, ovary, and prostate. KLF5 mediates the expression of several genes essential for proper cardiac structure and function, and plays a role in familial dilated cardiomyopathy. It functions as a transcriptional activator. KLF5 exhibits both transcriptional activation activity as well as trans-activating function. It belongs to a family of proteins called the Specificity Protein (SP)/KLF family, characterized by a C-terminal DNA-binding domain of 81 amino acids consisting of three Kruppel-like C2H2 zinc fingers. These factors bind to a loose consensus motif, namely NNRCRCCYY (where N is any nucleotide; R is A/G, and Y is C/T), such as the recurring motifs in GC and GT boxes (5'-GGGGCGGGG-3' and 5-GGTGTGGGG-3') that are present in promoters and more distal regulatory elements of mammalian genes. Members of the KLF family can act as activators or repressors of transcription depending on cell and promoter context. KLFs regulate various cellular functions, such as proliferation, differentiation, and apoptosis, as well as the development and homeostasis of several types of tissue. In addition to the C-terminal DNA-binding domain, each KLF also has a unique N-terminal activation/repression domain that confers specificity and allows it to bind specifically to a certain partner, leading to distinct activities in vivo. This model represents the N-terminal domain of KLF5.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 528468400   3 ATLLTMSAALAPGQDDFLSLLKPSLSDGFQEDSSLfCFEHKNPDSERGLSNDYSQAKLEMDKYLSPQP--VVLPDLKKSR 80
Cdd:cd21579    1 AAGLRMSAGLAPGEEAVFTQLKPVLMSGGDGEESL-LFGDKPAVRGRSSPHDYNQTKSEMDKYLSPQPppPPAPEDKKYR 79

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 528468400  81 RESVSTMDQYFGDDHGSPYSINMNVYLPDIAYLRTGLCRAARHSVSnQVKTEPVtapfsHPECPVSSSGPAPSALPDFTS 160
Cdd:cd21579   80 RESASVVDEFFSEDKGSPYSVNMNVFLPDVTYLRTGLCRPVRPIKT-EPKHEPP-----PPTSFCSSSNGTTQALPEFTS 153

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 528468400 161 IFNSNSTSGSgmdvvddsaGVFVKQEMQGFDIPQDGPLFQLLNSELDVpdptdphahsqmptfhnlhlaaqqtaaksycg 240
Cdd:cd21579  154 VFSAPQSAVN---------NVFIKQEMPSFDDQQQGPLFQLLNSDLDQ-------------------------------- 192

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 528468400 241 maahgyplshghfvhpqdqahsQLKMTYLPPSPPTSEPGSPDRQKELLHNLTPPPSYAATIASKMAGHAPSHPTP-ASAR 319
Cdd:cd21579  193 ----------------------QQQPTYLPPSPPNSEPGSPDRQKELLHNLSPPPSYAASIASKLAGQTPGLPPPgVGPL 250

                        330       340
                 ....*....|....*....|...
gi 528468400 320 PPTTTGSMPVRYNRRTNPDLEKR 342
Cdd:cd21579  251 SPGQAQSAPVRYNRRNNPDLEKR 273

FOG: Zn-finger [General function prediction only];

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 528468400 322 TTTGSMPVRYNRRTNPDLEKRRIHHCDFpgCKKVYTKSSHLKAHLRTHTGEKPYRCTWEGCDWRFARSDELTRHFRKHTG 401
Cdd:COG5048   11 SNNSVLSSTPKSTLKSLSNAPRPDSCPN--CTDSFSRLEHLTRHIRSHTGEKPSQCSYSGCDKSFSRPLELSRHLRTHHN 88

                 ....*..
gi 528468400 402 AKPFQCA 408
Cdd:COG5048   89 NPSDLNS 95

N-terminal domain of Kruppel-like factor 5; Kruppel-like factor 5 (KLF5; also known as also known as Krueppel-like factor 5; intestinal enriched Kruppel-like factor/IKLF; basic transcription element binding protein 2/BTEB2) a protein that in humans is encoded by the KLF5 gene. KLF5 is involved in numerous functions in eukaryotic cells, such as proliferation, migration, and differentiation. The loss of KLF5 expression is associated with tumors of the breast, cervix, endometrium, ovary, and prostate. KLF5 mediates the expression of several genes essential for proper cardiac structure and function, and plays a role in familial dilated cardiomyopathy. It functions as a transcriptional activator. KLF5 exhibits both transcriptional activation activity as well as trans-activating function. It belongs to a family of proteins called the Specificity Protein (SP)/KLF family, characterized by a C-terminal DNA-binding domain of 81 amino acids consisting of three Kruppel-like C2H2 zinc fingers. These factors bind to a loose consensus motif, namely NNRCRCCYY (where N is any nucleotide; R is A/G, and Y is C/T), such as the recurring motifs in GC and GT boxes (5'-GGGGCGGGG-3' and 5-GGTGTGGGG-3') that are present in promoters and more distal regulatory elements of mammalian genes. Members of the KLF family can act as activators or repressors of transcription depending on cell and promoter context. KLFs regulate various cellular functions, such as proliferation, differentiation, and apoptosis, as well as the development and homeostasis of several types of tissue. In addition to the C-terminal DNA-binding domain, each KLF also has a unique N-terminal activation/repression domain that confers specificity and allows it to bind specifically to a certain partner, leading to distinct activities in vivo. This model represents the N-terminal domain of KLF5.

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 528468400   3 ATLLTMSAALAPGQDDFLSLLKPSLSDGFQEDSSLfCFEHKNPDSERGLSNDYSQAKLEMDKYLSPQP--VVLPDLKKSR 80
Cdd:cd21579    1 AAGLRMSAGLAPGEEAVFTQLKPVLMSGGDGEESL-LFGDKPAVRGRSSPHDYNQTKSEMDKYLSPQPppPPAPEDKKYR 79

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 528468400  81 RESVSTMDQYFGDDHGSPYSINMNVYLPDIAYLRTGLCRAARHSVSnQVKTEPVtapfsHPECPVSSSGPAPSALPDFTS 160
Cdd:cd21579   80 RESASVVDEFFSEDKGSPYSVNMNVFLPDVTYLRTGLCRPVRPIKT-EPKHEPP-----PPTSFCSSSNGTTQALPEFTS 153

                        170       180       190       200       210       220       230       240
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 528468400 161 IFNSNSTSGSgmdvvddsaGVFVKQEMQGFDIPQDGPLFQLLNSELDVpdptdphahsqmptfhnlhlaaqqtaaksycg 240
Cdd:cd21579  154 VFSAPQSAVN---------NVFIKQEMPSFDDQQQGPLFQLLNSDLDQ-------------------------------- 192

                        250       260       270       280       290       300       310       320
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 528468400 241 maahgyplshghfvhpqdqahsQLKMTYLPPSPPTSEPGSPDRQKELLHNLTPPPSYAATIASKMAGHAPSHPTP-ASAR 319
Cdd:cd21579  193 ----------------------QQQPTYLPPSPPNSEPGSPDRQKELLHNLSPPPSYAASIASKLAGQTPGLPPPgVGPL 250

                        330       340
                 ....*....|....*....|...
gi 528468400 320 PPTTTGSMPVRYNRRTNPDLEKR 342
Cdd:cd21579  251 SPGQAQSAPVRYNRRNNPDLEKR 273

Zinc finger, C2H2 type; The C2H2 zinc finger is the classical zinc finger domain. The two conserved cysteines and histidines co-ordinate a zinc ion. The following pattern describes the zinc finger. #-X-C-X(1-5)-C-X3-#-X5-#-X2-H-X(3-6)-[H/C] Where X can be any amino acid, and numbers in brackets indicate the number of residues. The positions marked # are those that are important for the stable fold of the zinc finger. The final position can be either his or cys. The C2H2 zinc finger is composed of two short beta strands followed by an alpha helix. The amino terminal part of the helix binds the major groove in DNA binding zinc fingers. The accepted consensus binding sequence for Sp1 is usually defined by the asymmetric hexanucleotide core GGGCGG but this sequence does not include, among others, the GAG (=CTC) repeat that constitutes a high-affinity site for Sp1 binding to the wt1 promoter.

                          10        20
                  ....*....|....*....|...
gi 528468400  405 FQCAVCSRSFSRSDHLALHMKRH 427
Cdd:pfam00096   1 YKCPDCGKSFSRKSNLKRHLRTH 23

Zinc-finger double domain;

                          10        20
                  ....*....|....*....|....*...
gi 528468400  361 HLKAHLRTHTGEKPYRCtwEGCDWRFAR 388
Cdd:pfam13465   1 NLKRHMRTHTGEKPYKC--PECGKSFKS 26