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Conserved domains on  [gi|564332160|ref|XP_006230809|]
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crossover junction endonuclease MUS81 isoform X5 [Rattus norvegicus]

Protein Classification

crossover junction endonuclease MUS81( domain architecture ID 15338741)

crossover junction endonuclease MUS81 interacts with EME1 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
XPF_nuclease_Mus81 cd20074
XPF-like nuclease domain of Mus81; Mus81 is a crossover junction endonuclease that interacts ...
 251-387 2.39e-67

XPF-like nuclease domain of Mus81; Mus81 is a crossover junction endonuclease that interacts with Eme1 and Eme2 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. The typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. Mus81 may be required in mitosis for the processing of stalled or collapsed replication forks. Mus81 consists of the active nuclease domain with the GDX(n)ERKX(3)D motif which is required for metal-dependent endonuclease activity and two helix-hairpin-helix (HhH2) domains.


:

Pssm-ID: 410850 [Multi-domain]  Cd Length: 150  Bit Score: 213.50  E-value: 2.39e-67
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564332160 251 HRPEMLRELQRLRVPHTVRKLHVGDFVWVAqetRPRDPERpGELVLDHIVERKRLDDLCSSIIDGRFREQKFRLKRCGLG 330
Cdd:cd20074   18 DRDYFQRELEKLGVKVETRSLPVGDFLWVA---RHKSDTG-EELVLDYIVERKRLDDLASSIKDGRYHEQKFRLKRSGIK 93

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*..
gi 564332160 331 HRIYLVEEHGSVQNLSLPESTLLQAVTNTQVIDGFFVKRTMDIKESAGYLALLTKGL 387
Cdd:cd20074   94 NVIYLVEGDGSAQSGGLPEEALKTALANTQVVDGFFVKRTKSLDETVRYLARLTRAL 150
WH_MUS81 cd21036
winged helix domain found in crossover junction endonuclease MUS81 and similar proteins; MUS81 ...
 130-219 4.10e-22

winged helix domain found in crossover junction endonuclease MUS81 and similar proteins; MUS81 is a crossover junction endonuclease that interacts with EME1 (essential meiotic structure-specific endonuclease 1) and EME2, to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. The MUS80-EME1 endonuclease maintains genomic integrity in metazoans by cleaving branched DNA structures that can form during mitosis and fission yeast meiosis, and during processing of damaged replication folks. This model corresponds to the winged helix (WH) domain of MUS81, which is responsible for DNA binding. It comprises four helices and two beta strands.


:

Pssm-ID: 411029  Cd Length: 94  Bit Score: 90.67  E-value: 4.10e-22
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564332160 130 YWPAQNSGAREILLQLYREHLNsDGHSFLTKEELLQKcAQK---TPRVVPESSR---PWPALRGLLHRNLVLRTHRPARY 203
Cdd:cd21036    1 YVPKYRSGAYAILLALYKLDKE-PGKGGLTKEELIRR-AQPycdSSFTIPDPGSfytAWSSMKTLIKKGLVYKEGRPARY 78

                         90
                 ....*....|....*.
gi 564332160 204 ALTPEGLELAQKLAEA 219
Cdd:cd21036   79 SLTEEGRELAEKLAEA 94
 
Name Accession Description Interval E-value
XPF_nuclease_Mus81 cd20074
XPF-like nuclease domain of Mus81; Mus81 is a crossover junction endonuclease that interacts ...
 251-387 2.39e-67

XPF-like nuclease domain of Mus81; Mus81 is a crossover junction endonuclease that interacts with Eme1 and Eme2 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. The typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. Mus81 may be required in mitosis for the processing of stalled or collapsed replication forks. Mus81 consists of the active nuclease domain with the GDX(n)ERKX(3)D motif which is required for metal-dependent endonuclease activity and two helix-hairpin-helix (HhH2) domains.


Pssm-ID: 410850 [Multi-domain]  Cd Length: 150  Bit Score: 213.50  E-value: 2.39e-67
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564332160 251 HRPEMLRELQRLRVPHTVRKLHVGDFVWVAqetRPRDPERpGELVLDHIVERKRLDDLCSSIIDGRFREQKFRLKRCGLG 330
Cdd:cd20074   18 DRDYFQRELEKLGVKVETRSLPVGDFLWVA---RHKSDTG-EELVLDYIVERKRLDDLASSIKDGRYHEQKFRLKRSGIK 93

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*..
gi 564332160 331 HRIYLVEEHGSVQNLSLPESTLLQAVTNTQVIDGFFVKRTMDIKESAGYLALLTKGL 387
Cdd:cd20074   94 NVIYLVEGDGSAQSGGLPEEALKTALANTQVVDGFFVKRTKSLDETVRYLARLTRAL 150
ERCC4 pfam02732
ERCC4 domain; This domain is a family of nucleases. The family includes EME1 which is an ...
 257-383 4.10e-32

ERCC4 domain; This domain is a family of nucleases. The family includes EME1 which is an essential component of a Holliday junction resolvase. EME1 interacts with MUS81 to form a DNA structure-specific endonuclease.


Pssm-ID: 426945 [Multi-domain]  Cd Length: 139  Bit Score: 120.23  E-value: 4.10e-32
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564332160  257 RELQRLRVPHTVRKLHVGDFVWVAQETRPRdperpGELVLDHIVERKRLDDLCSSIIDGRFREQKFRLKRcGLGHRIYLV 336
Cdd:pfam02732  13 LLLEELGVEVVVETLPVGDYLWVPREYDLE-----LEVVLDVIVERKSLDDLVSSIIDGRLFEQKSRLKR-GYKKPILLV 86

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|...
gi 564332160  337 EEHGSVQN------LSLPESTLLQAVTNTQVIDGFFVKRTMDIKESAGYLALL 383
Cdd:pfam02732  87 EGLDLFSRklknkrRDINPNAIEGALASLQVDYGVRIIRTRSAEETAEWLASL 139
ERCC4 smart00891
ERCC4 domain; This entry represents a structural motif found in several DNA repair nucleases, ...
 254-337 8.52e-26

ERCC4 domain; This entry represents a structural motif found in several DNA repair nucleases, such as Rad1/Mus81/XPF endonucleases, and in ATP-dependent helicases. The XPF/Rad1/Mus81-dependent nuclease family specifically cleaves branched structures generated during DNA repair, replication, and recombination, and is essential for maintaining genome stability. The nuclease domain architecture exhibits remarkable similarity to those of restriction endonucleases.


Pssm-ID: 214888 [Multi-domain]  Cd Length: 98  Bit Score: 101.27  E-value: 8.52e-26
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564332160   254 EMLRELQ-RLRVPHTVRKLHVGDFVWVAQETRPRDPErpgELVLDHIVERKRLDDLCSSIIDGRFREQKFRLKRCGLGHR 332
Cdd:smart00891  16 PIPRSLRwKRGVKVEYDRLEAGDFVLVARDKQSLLPH---VNSLNELVERKSLTDLVASIPDGRLFEQVRRLQQIAYPSP 92


                   ....*
gi 564332160   333 IYLVE 337
Cdd:smart00891  93 QLLVE 97
WH_MUS81 cd21036
winged helix domain found in crossover junction endonuclease MUS81 and similar proteins; MUS81 ...
 130-219 4.10e-22

winged helix domain found in crossover junction endonuclease MUS81 and similar proteins; MUS81 is a crossover junction endonuclease that interacts with EME1 (essential meiotic structure-specific endonuclease 1) and EME2, to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. The MUS80-EME1 endonuclease maintains genomic integrity in metazoans by cleaving branched DNA structures that can form during mitosis and fission yeast meiosis, and during processing of damaged replication folks. This model corresponds to the winged helix (WH) domain of MUS81, which is responsible for DNA binding. It comprises four helices and two beta strands.


Pssm-ID: 411029  Cd Length: 94  Bit Score: 90.67  E-value: 4.10e-22
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564332160 130 YWPAQNSGAREILLQLYREHLNsDGHSFLTKEELLQKcAQK---TPRVVPESSR---PWPALRGLLHRNLVLRTHRPARY 203
Cdd:cd21036    1 YVPKYRSGAYAILLALYKLDKE-PGKGGLTKEELIRR-AQPycdSSFTIPDPGSfytAWSSMKTLIKKGLVYKEGRPARY 78

                         90
                 ....*....|....*.
gi 564332160 204 ALTPEGLELAQKLAEA 219
Cdd:cd21036   79 SLTEEGRELAEKLAEA 94
MUS81 COG1948
ERCC4-type crossover junction endonuclease [Replication, recombination and repair];
252-474 3.34e-07

ERCC4-type crossover junction endonuclease [Replication, recombination and repair];


Pssm-ID: 441551 [Multi-domain]  Cd Length: 214  Bit Score: 50.95  E-value: 3.34e-07
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564332160 252 RPEMLRELQRLRVPHTVRKLHVGDFVwvaqetrprdperpgelVLDHI-VERKRLDDLCSSIIDGRFREQKFRLKRCgLG 330
Cdd:COG1948   12 NSGVPRLLSRLGVEVRVKTLEVGDYV-----------------VSDRVaVERKTVRDFVNSLIDGRLFEQASRLAEA-YE 73

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564332160 331 HRIYLVEEHGSVQNLSLPESTLLQAVTNTQVIDGFFVKRTMDIKESAGYLALLTKGL-ERLYQGHTLHSRPwgtpgdaes 409
Cdd:COG1948   74 RPVLIIEGDLLYEERNIHPNAIRGALASLALDFGIPVLPTRDAEDTAELLVTLARREqEEEKREVSLHGKK--------- 144

                        170       180       190       200       210       220
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 564332160 410 eakpstnplcslltfsdfnaeavknKAQSVREvfaRQLMQVRGLSG---EKAAALVDRYSTPASLLAA 474
Cdd:COG1948  145 -------------------------KPKTLRE---QQLYVVESLPGigpKLARRLLEHFGSVEAVFNA 184
PRK13766 PRK13766
Hef nuclease; Provisional
 206-326 1.55e-04

Hef nuclease; Provisional


Pssm-ID: 237496 [Multi-domain]  Cd Length: 773  Bit Score: 44.48  E-value: 1.55e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564332160 206 TPEGLELAQKLAEAEGL-STLNTAFQPEEHHEESPVPEAILSEPAGHRPE-----------MLRELQRLRVPHTVRKLHV 273
Cdd:PRK13766 515 DEEQKGEEEEKDEQLSLdDFVKSKGKEEEEEEEKEEKDKETEEDEPEGPKiivdsrelrsnVARHLKRLGAEVELKTLEV 594

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....
gi 564332160 274 GDFVwvaqetrprdperpgelVLDHI-VERKRLDDLCSSIIDGRFREQKFRLKR 326
Cdd:PRK13766 595 GDYV-----------------VSDRVaVERKTAEDFVDSIIDRRLFEQVKDLKR 631
 
Name Accession Description Interval E-value
XPF_nuclease_Mus81 cd20074
XPF-like nuclease domain of Mus81; Mus81 is a crossover junction endonuclease that interacts ...
 251-387 2.39e-67

XPF-like nuclease domain of Mus81; Mus81 is a crossover junction endonuclease that interacts with Eme1 and Eme2 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. The typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. Mus81 may be required in mitosis for the processing of stalled or collapsed replication forks. Mus81 consists of the active nuclease domain with the GDX(n)ERKX(3)D motif which is required for metal-dependent endonuclease activity and two helix-hairpin-helix (HhH2) domains.


Pssm-ID: 410850 [Multi-domain]  Cd Length: 150  Bit Score: 213.50  E-value: 2.39e-67
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564332160 251 HRPEMLRELQRLRVPHTVRKLHVGDFVWVAqetRPRDPERpGELVLDHIVERKRLDDLCSSIIDGRFREQKFRLKRCGLG 330
Cdd:cd20074   18 DRDYFQRELEKLGVKVETRSLPVGDFLWVA---RHKSDTG-EELVLDYIVERKRLDDLASSIKDGRYHEQKFRLKRSGIK 93

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*..
gi 564332160 331 HRIYLVEEHGSVQNLSLPESTLLQAVTNTQVIDGFFVKRTMDIKESAGYLALLTKGL 387
Cdd:cd20074   94 NVIYLVEGDGSAQSGGLPEEALKTALANTQVVDGFFVKRTKSLDETVRYLARLTRAL 150
XPF_ERCC4_MUS81-like cd22367
XPF family DNA repair endonuclease; (Xeroderma Pigmentosum group F) DNA repair gene homologs ...
 251-383 5.05e-34

XPF family DNA repair endonuclease; (Xeroderma Pigmentosum group F) DNA repair gene homologs are members of the XPF/Rad1/Mus81-dependent nuclease family which specifically cleave branched structures generated during DNA repair, replication, and recombination, and they are essential for maintaining genome stability. They belong to a wider superfamily of nucleases including very short patch repair (Vsr) endonucleases, archaeal Holliday junction resolvases, MutH methyl-directed DNA mismatch-repair endonucleases, and catalytic domains of many restriction endonucleases, such as EcoRI, BamHI, and FokI.


Pssm-ID: 411771 [Multi-domain]  Cd Length: 123  Bit Score: 124.68  E-value: 5.05e-34
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564332160 251 HRPEMLRELQRLRVPHTVRKLHVGDFVWVAqetrprdperpgelvlDHIVERKRLDDLCSSIIDGRFREQKFRLKRCGLg 330
Cdd:cd22367    8 RRSGLPELLRKLGVRVEVRTLEVGDYILSA----------------DIIVERKTVSDLISSIIDGRLFEQAERLKRSYE- 70

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|...
gi 564332160 331 HRIYLVEEHGSVQNLSLPESTLLQAVTNTQVIDGFFVKRTMDIKESAGYLALL 383
Cdd:cd22367   71 RPILLIEGDPDKARRLVRPAALGAAISSLLVIGGLLVLRTPNFETTALLLSLL 123
XPF_nuclease-like cd19940
nuclease domain of XPF/MUS81 family proteins; The XPF/MUS81 family belongs to 3'-flap ...
 252-385 1.01e-33

nuclease domain of XPF/MUS81 family proteins; The XPF/MUS81 family belongs to 3'-flap endonuclease that act upon 3'-flap structures and involved in DNA repair pathways that are necessary for the removal of UV-light-induced DNA lesions and cross-links between DNA strands. Family members exist either as heterodimers or as homodimers in their functionally competent states which consist of a catalytic and a noncatalytic subunit. The catalytic subunits have a DX(n)RKX(3)D motif. This motif is required for metal-dependent endonuclease activity but not for DNA junction binding. The equivalent regions of the noncatalytic subunits (ERCC1, EME1, and FAAP24) have diverged. The noncatalytic subunits have roles such as binding ssDNA or an ability to target the endonuclease to defined DNA structures or sites of DNA damage.


Pssm-ID: 410849 [Multi-domain]  Cd Length: 126  Bit Score: 124.03  E-value: 1.01e-33
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564332160 252 RPEMLRELQRLRVPHTVRKLHVGDFVWVAqetrprdperpgelvlDHIVERKRLDDLCSSIIDGRFREQKFRLKRCGLgH 331
Cdd:cd19940   10 RSELLSELQRLGVQVEFEDLAVGDYVLSN----------------RTCVERKSLSDLVSSINKGRLREQLQRLTRKFE-R 72

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....
gi 564332160 332 RIYLVEEHGSVQNLSLPESTLLQAVTNTQVIDGFFVKRTMDIKESAGYLALLTK 385
Cdd:cd19940   73 RVLLVEKDRSKFRSMVSSVQALSALTKLQLLTGIRLLIVASPKETADLLEELTQ 126
ERCC4 pfam02732
ERCC4 domain; This domain is a family of nucleases. The family includes EME1 which is an ...
 257-383 4.10e-32

ERCC4 domain; This domain is a family of nucleases. The family includes EME1 which is an essential component of a Holliday junction resolvase. EME1 interacts with MUS81 to form a DNA structure-specific endonuclease.


Pssm-ID: 426945 [Multi-domain]  Cd Length: 139  Bit Score: 120.23  E-value: 4.10e-32
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564332160  257 RELQRLRVPHTVRKLHVGDFVWVAQETRPRdperpGELVLDHIVERKRLDDLCSSIIDGRFREQKFRLKRcGLGHRIYLV 336
Cdd:pfam02732  13 LLLEELGVEVVVETLPVGDYLWVPREYDLE-----LEVVLDVIVERKSLDDLVSSIIDGRLFEQKSRLKR-GYKKPILLV 86

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|...
gi 564332160  337 EEHGSVQN------LSLPESTLLQAVTNTQVIDGFFVKRTMDIKESAGYLALL 383
Cdd:pfam02732  87 EGLDLFSRklknkrRDINPNAIEGALASLQVDYGVRIIRTRSAEETAEWLASL 139
ERCC4 smart00891
ERCC4 domain; This entry represents a structural motif found in several DNA repair nucleases, ...
 254-337 8.52e-26

ERCC4 domain; This entry represents a structural motif found in several DNA repair nucleases, such as Rad1/Mus81/XPF endonucleases, and in ATP-dependent helicases. The XPF/Rad1/Mus81-dependent nuclease family specifically cleaves branched structures generated during DNA repair, replication, and recombination, and is essential for maintaining genome stability. The nuclease domain architecture exhibits remarkable similarity to those of restriction endonucleases.


Pssm-ID: 214888 [Multi-domain]  Cd Length: 98  Bit Score: 101.27  E-value: 8.52e-26
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564332160   254 EMLRELQ-RLRVPHTVRKLHVGDFVWVAQETRPRDPErpgELVLDHIVERKRLDDLCSSIIDGRFREQKFRLKRCGLGHR 332
Cdd:smart00891  16 PIPRSLRwKRGVKVEYDRLEAGDFVLVARDKQSLLPH---VNSLNELVERKSLTDLVASIPDGRLFEQVRRLQQIAYPSP 92


                   ....*
gi 564332160   333 IYLVE 337
Cdd:smart00891  93 QLLVE 97
WH_MUS81 cd21036
winged helix domain found in crossover junction endonuclease MUS81 and similar proteins; MUS81 ...
 130-219 4.10e-22

winged helix domain found in crossover junction endonuclease MUS81 and similar proteins; MUS81 is a crossover junction endonuclease that interacts with EME1 (essential meiotic structure-specific endonuclease 1) and EME2, to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. The MUS80-EME1 endonuclease maintains genomic integrity in metazoans by cleaving branched DNA structures that can form during mitosis and fission yeast meiosis, and during processing of damaged replication folks. This model corresponds to the winged helix (WH) domain of MUS81, which is responsible for DNA binding. It comprises four helices and two beta strands.


Pssm-ID: 411029  Cd Length: 94  Bit Score: 90.67  E-value: 4.10e-22
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564332160 130 YWPAQNSGAREILLQLYREHLNsDGHSFLTKEELLQKcAQK---TPRVVPESSR---PWPALRGLLHRNLVLRTHRPARY 203
Cdd:cd21036    1 YVPKYRSGAYAILLALYKLDKE-PGKGGLTKEELIRR-AQPycdSSFTIPDPGSfytAWSSMKTLIKKGLVYKEGRPARY 78

                         90
                 ....*....|....*.
gi 564332160 204 ALTPEGLELAQKLAEA 219
Cdd:cd21036   79 SLTEEGRELAEKLAEA 94
XPF_nuclease_XPF_arch cd20075
nuclease domain of XPF found in archaea; XPF, also called DNA excision repair protein ERCC-4, ...
 252-326 1.45e-08

nuclease domain of XPF found in archaea; XPF, also called DNA excision repair protein ERCC-4, or DNA repair protein complementing XP-F cells, or Xeroderma pigmentosum group F-complementing protein, is a 3'-flap repair endonuclease that cleaves 5' of ds/ssDNA interfaces in 3' flap structures, although it also cuts bubble, Y-DNA structures and mobile and immobile Holliday junctions. XPF cuts preferentially after pyrimidines, may continue to progressively cleave substrate upstream of the initial cleavage, at least in vitro. It may be involved in nucleotide excision repair. The nuclease domains of the catalytic subunits XPF have the GDX(n)ERKX(3)D motif which is required for metal-dependent endonuclease activity but not for DNA junction binding. XPF-ERRC1 and its yeast homolog Rad1-Rad10 play key roles in the excision of DNA lesions and are required for certain types of homologous recombination events and for the repair of DNA cross-links.


Pssm-ID: 410851 [Multi-domain]  Cd Length: 127  Bit Score: 53.16  E-value: 1.45e-08
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 564332160 252 RPEMLRELQRLRVPHTVRKLHVGDFVwVAQETrprdperpgelvldhIVERKRLDDLCSSIIDGRFREQKFRLKR 326
Cdd:cd20075   10 NSGVVRELKELGVEVEFKQLEVGDYI-VSDRV---------------AIERKTVDDFVSSIIDGRLFDQAKRLKE 68
MUS81 COG1948
ERCC4-type crossover junction endonuclease [Replication, recombination and repair];
252-474 3.34e-07

ERCC4-type crossover junction endonuclease [Replication, recombination and repair];


Pssm-ID: 441551 [Multi-domain]  Cd Length: 214  Bit Score: 50.95  E-value: 3.34e-07
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564332160 252 RPEMLRELQRLRVPHTVRKLHVGDFVwvaqetrprdperpgelVLDHI-VERKRLDDLCSSIIDGRFREQKFRLKRCgLG 330
Cdd:COG1948   12 NSGVPRLLSRLGVEVRVKTLEVGDYV-----------------VSDRVaVERKTVRDFVNSLIDGRLFEQASRLAEA-YE 73

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564332160 331 HRIYLVEEHGSVQNLSLPESTLLQAVTNTQVIDGFFVKRTMDIKESAGYLALLTKGL-ERLYQGHTLHSRPwgtpgdaes 409
Cdd:COG1948   74 RPVLIIEGDLLYEERNIHPNAIRGALASLALDFGIPVLPTRDAEDTAELLVTLARREqEEEKREVSLHGKK--------- 144

                        170       180       190       200       210       220
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 564332160 410 eakpstnplcslltfsdfnaeavknKAQSVREvfaRQLMQVRGLSG---EKAAALVDRYSTPASLLAA 474
Cdd:COG1948  145 -------------------------KPKTLRE---QQLYVVESLPGigpKLARRLLEHFGSVEAVFNA 184
PRK13766 PRK13766
Hef nuclease; Provisional
 206-326 1.55e-04

Hef nuclease; Provisional


Pssm-ID: 237496 [Multi-domain]  Cd Length: 773  Bit Score: 44.48  E-value: 1.55e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 564332160 206 TPEGLELAQKLAEAEGL-STLNTAFQPEEHHEESPVPEAILSEPAGHRPE-----------MLRELQRLRVPHTVRKLHV 273
Cdd:PRK13766 515 DEEQKGEEEEKDEQLSLdDFVKSKGKEEEEEEEKEEKDKETEEDEPEGPKiivdsrelrsnVARHLKRLGAEVELKTLEV 594

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....
gi 564332160 274 GDFVwvaqetrprdperpgelVLDHI-VERKRLDDLCSSIIDGRFREQKFRLKR 326
Cdd:PRK13766 595 GDYV-----------------VSDRVaVERKTAEDFVDSIIDRRLFEQVKDLKR 631
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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