C-terminal double-RWD domain of kinetochore scaffold 1 and related proteins; Kinetochore scaffold 1 (KNL1 or Knl1, also known as Spc105, Spc7, and Blinkin) coordinates the spindle assembly checkpoint (SAC), a signaling pathway that delays chromosome segregation until all sister chromatids are properly attached to spindle microtubules (MTs). Knl1 is part of the KMN network, a conserved kinetochore protein complex that connects kinetochores to MTs. Knl1 has been shown to play an effective role in decreasing apoptosis and promoting the proliferation of colorectal cancer cells, suggesting that its inhibition may represent a promising therapeutic approach for colorectal cancer patients. Knl1 contains tandem RWD domains, also known as a double-RWD domain, DRWD, which has been shown to bind the Mis12 complex, consisting of Dsn1 and Nsl1, that tethers directly onto the underlying chromatin layer and mediates kinetochore targeting of Knl1. The model corresponds to the C-terminal double-RWD domain (DRWD-C).

                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1195708174 2021 LLGEEIEFLKRWGPNYSLMHINVNNTELRLLFSSCAAFAKFEITLSPSAHYPLVPLPFTIHNHIGKTSHDEIAAIISKVP 2100
Cdd:cd22892      1 LLGEEIEYLKRWGGKYNILKIDINDTNVHLLFSSSAAFAKFEITLSLTSGYPSVPLPFTFENHIGNTSQDQIEEVISKVP 80

                           90
                   ....*....|....*...
gi 1195708174 2101 LEENYLKNVVKQIYQDLL 2118
Cdd:cd22892     81 PGKNYLKRVVKKIHQDLL 98

N-terminal domain found in kinetochore scaffold 1 (KNL1) and similar proteins; Kinetochore scaffold 1 (KNL1), also called ALL1-fused gene from chromosome 15q14 protein (AF15q14), Bub-linking kinetochore protein (Blinkin), cancer susceptibility candidate gene 5 protein (CASC5), cancer/testis antigen 29 (CT29), kinetochore-null protein 1, or protein D40/AF15q14, plays crucial roles during mitosis. It is essential for spindle-assembly checkpoint (SAC) signaling and for correct chromosome alignment. It is required for attachment of the kinetochores to the spindle microtubules. KNL1 directly links BUB1 and BUB1B to kinetochores. It is part of the MIS12 complex, which may be fundamental for kinetochore formation and proper chromosome segregation during mitosis. KNL1 also acts in coordination with CENPK to recruit the NDC80 complex to the outer kinetochore. This model corresponds to a small conserved region at the N-terminus of KNL1, which is responsible for the binding of protein phosphatase 1 (PP1). PP1 regulates SAC silencing and mitotic exit, and is targeted to kinetochores, in part, by KNL1.

                           10        20        30        40        50
                   ....*....|....*....|....*....|....*....|....*....|....*.
gi 1195708174   20 RRQHSSILKPPRSPLQDLKCGNQTNQEPNPPRKRKSSRRVSFADTIKVFQTESHMK 75
Cdd:cd21853      1 KRRRSSILKPPRSPLLDLDEGNEQNQDLNKKKKRRSSRRVSFADTIQVKEFEKDAK 56

MELT motif; The outer kinetochore protein scaffold KNL1 is essential for error-free chromosome segregation during mitosis and meiosis. A critical feature of KNL1 is an array of repeats containing MELT-like motifs. When phosphorylated, these motifs form docking sites for the BUB1-BUB3 dimer that regulates chromosome biorientation and the spindle assembly checkpoint. This entry mainly represents vertebrate proteins although MELT motifs are found much more widely.

                           10        20
                   ....*....|....*....|....*.
gi 1195708174  721 KIDKTILFSEgNEGDMDITKSCTVKI 746
Cdd:pfam19221    1 PGDKTVVFSS-DANDMDITKSHTVNI 25

MELT motif; The outer kinetochore protein scaffold KNL1 is essential for error-free chromosome segregation during mitosis and meiosis. A critical feature of KNL1 is an array of repeats containing MELT-like motifs. When phosphorylated, these motifs form docking sites for the BUB1-BUB3 dimer that regulates chromosome biorientation and the spindle assembly checkpoint. This entry mainly represents vertebrate proteins although MELT motifs are found much more widely.

                           10        20
                   ....*....|....*....|....
gi 1195708174  451 REKTIYL--EEDSMDITKSHTVAI 472
Cdd:pfam19221    2 GDKTVVFssDANDMDITKSHTVNI 25

MELT motif; The outer kinetochore protein scaffold KNL1 is essential for error-free chromosome segregation during mitosis and meiosis. A critical feature of KNL1 is an array of repeats containing MELT-like motifs. When phosphorylated, these motifs form docking sites for the BUB1-BUB3 dimer that regulates chromosome biorientation and the spindle assembly checkpoint. This entry mainly represents vertebrate proteins although MELT motifs are found much more widely.

                           10        20
                   ....*....|....*....|....*
gi 1195708174  245 NSNRTRIFREQDDGMNLTQCHTACI 269
Cdd:pfam19221    1 PGDKTVVFSSDANDMDITKSHTVNI 25

C-terminal double-RWD domain of kinetochore scaffold 1 and related proteins; Kinetochore scaffold 1 (KNL1 or Knl1, also known as Spc105, Spc7, and Blinkin) coordinates the spindle assembly checkpoint (SAC), a signaling pathway that delays chromosome segregation until all sister chromatids are properly attached to spindle microtubules (MTs). Knl1 is part of the KMN network, a conserved kinetochore protein complex that connects kinetochores to MTs. Knl1 has been shown to play an effective role in decreasing apoptosis and promoting the proliferation of colorectal cancer cells, suggesting that its inhibition may represent a promising therapeutic approach for colorectal cancer patients. Knl1 contains tandem RWD domains, also known as a double-RWD domain, DRWD, which has been shown to bind the Mis12 complex, consisting of Dsn1 and Nsl1, that tethers directly onto the underlying chromatin layer and mediates kinetochore targeting of Knl1. The model corresponds to the C-terminal double-RWD domain (DRWD-C).

                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1195708174 2021 LLGEEIEFLKRWGPNYSLMHINVNNTELRLLFSSCAAFAKFEITLSPSAHYPLVPLPFTIHNHIGKTSHDEIAAIISKVP 2100
Cdd:cd22892      1 LLGEEIEYLKRWGGKYNILKIDINDTNVHLLFSSSAAFAKFEITLSLTSGYPSVPLPFTFENHIGNTSQDQIEEVISKVP 80

                           90
                   ....*....|....*...
gi 1195708174 2101 LEENYLKNVVKQIYQDLL 2118
Cdd:cd22892     81 PGKNYLKRVVKKIHQDLL 98

Knl1 RWD C-terminal domain; This domain is found in Knl1, a sub-unit of the KMN network, present in Homo sapiens. The KMN network is the core of the outer kinetochore which is responsible for microtubule binding/stabilization and controls the spindle assembly checkpoint. This domain is the second of two RING finger, WD repeat, DEAD-like helicase (RWD) domains. The tandem RWD domains mediate kinetochore targeting of the microtubule-binding subunits by interacting with the Mis12 complex. The Mis12 complex is a KMN sub-complex that tethers directly onto the underlying chromatin layer.

                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1195708174 1880 RKFLEVETQKTQTLAQIE---------FIKEQTTKTEELLDQL-SLSEWDVIEWSDDQAVFTFVYDsIELIITFGeplvg 1949
Cdd:pfam18210   11 EKLEELEERKQELLAAIGeaerireecWTSEEVLRLKEELEALeSLHGWRITEVSDDTLVFTYLSD-IELTFDFG----- 84

                           90       100       110       120       130       140       150
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1195708174 1950 lpfldkACRKINALSFQSLLDEDKAPPSSLLVHKLIFQYIEEQESWKKKCTAQHQVPQMLQELSLVVNHCRLLG 2023
Cdd:pfam18210   85 ------ASPKISSIDLESYLDDEKAPPSSLLVHRLAFFFLQSARDWVRKYPTQTSLPKLLQDVSLVWSRCRLLG 152

MELT motif; The outer kinetochore protein scaffold KNL1 is essential for error-free chromosome segregation during mitosis and meiosis. A critical feature of KNL1 is an array of repeats containing MELT-like motifs. When phosphorylated, these motifs form docking sites for the BUB1-BUB3 dimer that regulates chromosome biorientation and the spindle assembly checkpoint. This entry mainly represents vertebrate proteins although MELT motifs are found much more widely.

                           10        20
                   ....*....|....*....|....*
gi 1195708174 1003 PMDKTVMFVDNLGDLDVTRSHTVFI 1027
Cdd:pfam19221    1 PGDKTVVFSSDANDMDITKSHTVNI 25

MELT motif; The outer kinetochore protein scaffold KNL1 is essential for error-free chromosome segregation during mitosis and meiosis. A critical feature of KNL1 is an array of repeats containing MELT-like motifs. When phosphorylated, these motifs form docking sites for the BUB1-BUB3 dimer that regulates chromosome biorientation and the spindle assembly checkpoint. This entry mainly represents vertebrate proteins although MELT motifs are found much more widely.

                           10        20
                   ....*....|....*....|...
gi 1195708174  804 NKTVMSIDDHEELEMTKSHTVFI 826
Cdd:pfam19221    3 DKTVVFSSDANDMDITKSHTVNI 25

MELT motif; The outer kinetochore protein scaffold KNL1 is essential for error-free chromosome segregation during mitosis and meiosis. A critical feature of KNL1 is an array of repeats containing MELT-like motifs. When phosphorylated, these motifs form docking sites for the BUB1-BUB3 dimer that regulates chromosome biorientation and the spindle assembly checkpoint. This entry mainly represents vertebrate proteins although MELT motifs are found much more widely.

                           10        20
                   ....*....|....*....|....
gi 1195708174  115 NDQTVIF-SDENQMDLTASHTIMI 137
Cdd:pfam19221    2 GDKTVVFsSDANDMDITKSHTVNI 25

MELT motif; The outer kinetochore protein scaffold KNL1 is essential for error-free chromosome segregation during mitosis and meiosis. A critical feature of KNL1 is an array of repeats containing MELT-like motifs. When phosphorylated, these motifs form docking sites for the BUB1-BUB3 dimer that regulates chromosome biorientation and the spindle assembly checkpoint. This entry mainly represents vertebrate proteins although MELT motifs are found much more widely.

                           10        20
                   ....*....|....*....|....*
gi 1195708174  619 CSHHKIMYPEELQTMDLTKSHTIVI 643
Cdd:pfam19221    1 PGDKTVVFSSDANDMDITKSHTVNI 25

C-terminal double-RWD domain of kinetochore scaffold 1 and related proteins; Kinetochore scaffold 1 (KNL1 or Knl1, also known as Spc105, Spc7, and Blinkin) coordinates the spindle assembly checkpoint (SAC), a signaling pathway that delays chromosome segregation until all sister chromatids are properly attached to spindle microtubules (MTs). Knl1 is part of the KMN network, a conserved kinetochore protein complex that connects kinetochores to MTs. Knl1 has been shown to play an effective role in decreasing apoptosis and promoting the proliferation of colorectal cancer cells, suggesting that its inhibition may represent a promising therapeutic approach for colorectal cancer patients. Knl1 contains tandem RWD domains, also known as a double-RWD domain, DRWD, which has been shown to bind the Mis12 complex, consisting of Dsn1 and Nsl1, that tethers directly onto the underlying chromatin layer and mediates kinetochore targeting of Knl1. The model corresponds to the C-terminal double-RWD domain (DRWD-C).

                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1195708174 2021 LLGEEIEFLKRWGPNYSLMHINVNNTELRLLFSSCAAFAKFEITLSPSAHYPLVPLPFTIHNHIGKTSHDEIAAIISKVP 2100
Cdd:cd22892      1 LLGEEIEYLKRWGGKYNILKIDINDTNVHLLFSSSAAFAKFEITLSLTSGYPSVPLPFTFENHIGNTSQDQIEEVISKVP 80

                           90
                   ....*....|....*...
gi 1195708174 2101 LEENYLKNVVKQIYQDLL 2118
Cdd:cd22892     81 PGKNYLKRVVKKIHQDLL 98

Knl1 RWD C-terminal domain; This domain is found in Knl1, a sub-unit of the KMN network, present in Homo sapiens. The KMN network is the core of the outer kinetochore which is responsible for microtubule binding/stabilization and controls the spindle assembly checkpoint. This domain is the second of two RING finger, WD repeat, DEAD-like helicase (RWD) domains. The tandem RWD domains mediate kinetochore targeting of the microtubule-binding subunits by interacting with the Mis12 complex. The Mis12 complex is a KMN sub-complex that tethers directly onto the underlying chromatin layer.

                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1195708174 1880 RKFLEVETQKTQTLAQIE---------FIKEQTTKTEELLDQL-SLSEWDVIEWSDDQAVFTFVYDsIELIITFGeplvg 1949
Cdd:pfam18210   11 EKLEELEERKQELLAAIGeaerireecWTSEEVLRLKEELEALeSLHGWRITEVSDDTLVFTYLSD-IELTFDFG----- 84

                           90       100       110       120       130       140       150
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1195708174 1950 lpfldkACRKINALSFQSLLDEDKAPPSSLLVHKLIFQYIEEQESWKKKCTAQHQVPQMLQELSLVVNHCRLLG 2023
Cdd:pfam18210   85 ------ASPKISSIDLESYLDDEKAPPSSLLVHRLAFFFLQSARDWVRKYPTQTSLPKLLQDVSLVWSRCRLLG 152

MELT motif; The outer kinetochore protein scaffold KNL1 is essential for error-free chromosome segregation during mitosis and meiosis. A critical feature of KNL1 is an array of repeats containing MELT-like motifs. When phosphorylated, these motifs form docking sites for the BUB1-BUB3 dimer that regulates chromosome biorientation and the spindle assembly checkpoint. This entry mainly represents vertebrate proteins although MELT motifs are found much more widely.

                           10        20
                   ....*....|....*....|....*
gi 1195708174 1003 PMDKTVMFVDNLGDLDVTRSHTVFI 1027
Cdd:pfam19221    1 PGDKTVVFSSDANDMDITKSHTVNI 25

MELT motif; The outer kinetochore protein scaffold KNL1 is essential for error-free chromosome segregation during mitosis and meiosis. A critical feature of KNL1 is an array of repeats containing MELT-like motifs. When phosphorylated, these motifs form docking sites for the BUB1-BUB3 dimer that regulates chromosome biorientation and the spindle assembly checkpoint. This entry mainly represents vertebrate proteins although MELT motifs are found much more widely.

                           10        20
                   ....*....|....*....|...
gi 1195708174  804 NKTVMSIDDHEELEMTKSHTVFI 826
Cdd:pfam19221    3 DKTVVFSSDANDMDITKSHTVNI 25

MELT motif; The outer kinetochore protein scaffold KNL1 is essential for error-free chromosome segregation during mitosis and meiosis. A critical feature of KNL1 is an array of repeats containing MELT-like motifs. When phosphorylated, these motifs form docking sites for the BUB1-BUB3 dimer that regulates chromosome biorientation and the spindle assembly checkpoint. This entry mainly represents vertebrate proteins although MELT motifs are found much more widely.

                           10        20
                   ....*....|....*....|....
gi 1195708174  115 NDQTVIF-SDENQMDLTASHTIMI 137
Cdd:pfam19221    2 GDKTVVFsSDANDMDITKSHTVNI 25

MELT motif; The outer kinetochore protein scaffold KNL1 is essential for error-free chromosome segregation during mitosis and meiosis. A critical feature of KNL1 is an array of repeats containing MELT-like motifs. When phosphorylated, these motifs form docking sites for the BUB1-BUB3 dimer that regulates chromosome biorientation and the spindle assembly checkpoint. This entry mainly represents vertebrate proteins although MELT motifs are found much more widely.

                           10        20
                   ....*....|....*....|....*
gi 1195708174  619 CSHHKIMYPEELQTMDLTKSHTIVI 643
Cdd:pfam19221    1 PGDKTVVFSSDANDMDITKSHTVNI 25