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Conserved domains on  [gi|2462538466|ref|XP_054231182|]
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peptide chain release factor 1, mitochondrial isoform X1 [Homo sapiens]

Protein Classification

peptide chain release factor 1( domain architecture ID 1907968)

peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

CATH:  3.30.160.20|3.30.70.1660|1.10.287.4000
Gene Ontology:  GO:0003747|GO:0016149
SCOP:  4002864|4002570

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
PrfA super family cl43066
Protein chain release factor RF1 [Translation, ribosomal structure and biogenesis]; Protein ...
 131-488 1.47e-138

Protein chain release factor RF1 [Translation, ribosomal structure and biogenesis]; Protein chain release factor RF1 is part of the Pathway/BioSystem: Translation factors


The actual alignment was detected with superfamily member COG0216:

Pssm-ID: 439986 [Multi-domain]  Cd Length: 356  Bit Score: 403.23  E-value: 1.47e-138
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462538466 131 MENLSKEYQTLEQCLQ--HIPVNEENRRSLNRRHAELAPLAAIYQEIQETEQAIEELESMcksLNKQDEKQLQELALEER 208
Cdd:COG0216     6 LEALEERYEELEALLSdpEVISDQKRFRKLSKEYAELEPIVEAYREYKKLLEDIEEAKEL---LEEESDPEMREMAKEEL 82

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462538466 209 QTIDQKINMLYNELFQSLVPKEKYDKNDVILEVTAGrtTGGD---IcqqFTREIFDMYQNYSCYKHWQFELLNYTPADYG 285
Cdd:COG0216    83 EELEARLEELEEELKILLLPKDPNDDKNVILEIRAG--TGGDeaaL---FAGDLFRMYSRYAERKGWKVEILSASEGELG 157

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462538466 286 GLHHAAARISGDGVYKHLKYEGGIHRVQRIPEV---GlssrmqRIHTGTMSVIVLPQPDEVDVKLDPKDLRIDTFRAKGA 362
Cdd:COG0216   158 GYKEVIFRISGEGVYSRLKFESGVHRVQRVPETesqG------RIHTSAATVAVLPEAEEVDVEINPKDLRIDTFRSSGA 231

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462538466 363 GGQHVNKTDSAVRLVHIPTGLVVECQQERSQIKNKEIAFRVLRARLYQQIIEKDKRQQQSARKLQVGTRAQSERIRTYNF 442
Cdd:COG0216   232 GGQHVNTTDSAVRITHLPTGIVVECQDERSQHKNKAKAMKVLRARLYEAEQQKQQAEIAAERKSQVGSGDRSERIRTYNF 311

                         330       340       350       360
                  ....*....|....*....|....*....|....*....|....*.
gi 2462538466 443 TQDRVSDHRIAYEVRDIKEFLCGgkGLDQLIQRLLQSADEEAIAEL 488
Cdd:COG0216   312 PQGRVTDHRINLTLYKLDEVMEG--DLDELIDALIAEDQAEKLAAL 355
 
Name Accession Description Interval E-value
PrfA COG0216
Protein chain release factor RF1 [Translation, ribosomal structure and biogenesis]; Protein ...
 131-488 1.47e-138

Protein chain release factor RF1 [Translation, ribosomal structure and biogenesis]; Protein chain release factor RF1 is part of the Pathway/BioSystem: Translation factors


Pssm-ID: 439986 [Multi-domain]  Cd Length: 356  Bit Score: 403.23  E-value: 1.47e-138
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462538466 131 MENLSKEYQTLEQCLQ--HIPVNEENRRSLNRRHAELAPLAAIYQEIQETEQAIEELESMcksLNKQDEKQLQELALEER 208
Cdd:COG0216     6 LEALEERYEELEALLSdpEVISDQKRFRKLSKEYAELEPIVEAYREYKKLLEDIEEAKEL---LEEESDPEMREMAKEEL 82

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462538466 209 QTIDQKINMLYNELFQSLVPKEKYDKNDVILEVTAGrtTGGD---IcqqFTREIFDMYQNYSCYKHWQFELLNYTPADYG 285
Cdd:COG0216    83 EELEARLEELEEELKILLLPKDPNDDKNVILEIRAG--TGGDeaaL---FAGDLFRMYSRYAERKGWKVEILSASEGELG 157

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462538466 286 GLHHAAARISGDGVYKHLKYEGGIHRVQRIPEV---GlssrmqRIHTGTMSVIVLPQPDEVDVKLDPKDLRIDTFRAKGA 362
Cdd:COG0216   158 GYKEVIFRISGEGVYSRLKFESGVHRVQRVPETesqG------RIHTSAATVAVLPEAEEVDVEINPKDLRIDTFRSSGA 231

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462538466 363 GGQHVNKTDSAVRLVHIPTGLVVECQQERSQIKNKEIAFRVLRARLYQQIIEKDKRQQQSARKLQVGTRAQSERIRTYNF 442
Cdd:COG0216   232 GGQHVNTTDSAVRITHLPTGIVVECQDERSQHKNKAKAMKVLRARLYEAEQQKQQAEIAAERKSQVGSGDRSERIRTYNF 311

                         330       340       350       360
                  ....*....|....*....|....*....|....*....|....*.
gi 2462538466 443 TQDRVSDHRIAYEVRDIKEFLCGgkGLDQLIQRLLQSADEEAIAEL 488
Cdd:COG0216   312 PQGRVTDHRINLTLYKLDEVMEG--DLDELIDALIAEDQAEKLAAL 355
prfA PRK00591
peptide chain release factor 1; Validated
 131-488 1.42e-135

peptide chain release factor 1; Validated


Pssm-ID: 234801 [Multi-domain]  Cd Length: 359  Bit Score: 395.60  E-value: 1.42e-135
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462538466 131 MENLSKEYQTLEQCLQ--HIPVNEENRRSLNRRHAELAPLAAIYQEIQETEQAIEELESMcksLNKQDEKQLQELALEER 208
Cdd:PRK00591    8 LEALEERYEELEALLSdpEVISDQKRFRKLSKEYAELEPIVEAYREYKQAQEDLEEAKEM---LEEESDPEMREMAKEEL 84

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462538466 209 QTIDQKINMLYNELFQSLVPKEKYDKNDVILEVTAGrtTGGDICQQFTREIFDMYQNYSCYKHWQFELLNYTPADYGGLH 288
Cdd:PRK00591   85 KELEERLEELEEELKILLLPKDPNDDKNVILEIRAG--TGGDEAALFAGDLFRMYSRYAERQGWKVEILSASEGELGGYK 162

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462538466 289 HAAARISGDGVYKHLKYEGGIHRVQRIPEV---GlssrmqRIHTGTMSVIVLPQPDEVDVKLDPKDLRIDTFRAKGAGGQ 365
Cdd:PRK00591  163 EVIAEISGDGVYSKLKFESGVHRVQRVPATesqG------RIHTSAATVAVLPEAEEVEVEINPKDLRIDTFRSSGAGGQ 236

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462538466 366 HVNKTDSAVRLVHIPTGLVVECQQERSQIKNKEIAFRVLRARLYQQIIEKDKRQQQSARKLQVGTRAQSERIRTYNFTQD 445
Cdd:PRK00591  237 HVNTTDSAVRITHLPTGIVVECQDERSQHKNKAKAMKVLRARLYDAERQKAQAEEAATRKSQVGSGDRSERIRTYNFPQG 316

                         330       340       350       360
                  ....*....|....*....|....*....|....*....|...
gi 2462538466 446 RVSDHRIAYEVRDIKEFLCGgkGLDQLIQRLLQSADEEAIAEL 488
Cdd:PRK00591  317 RVTDHRINLTLYKLDEVMEG--DLDELIDALIAEDQAEKLAAL 357
prfA TIGR00019
peptide chain release factor 1; This model describes peptide chain release factor 1 (PrfA, ...
 125-491 1.78e-114

peptide chain release factor 1; This model describes peptide chain release factor 1 (PrfA, RF-1), and excludes the related peptide chain release factor 2 (PrfB, RF-2). RF-1 helps recognize and terminate translation at UAA and UAG stop codons. The mitochondrial release factors are prfA-like, although not included above the trusted cutoff for this model. RF-1 does not have a translational frameshift. [Protein synthesis, Translation factors]


Pssm-ID: 129130 [Multi-domain]  Cd Length: 360  Bit Score: 342.06  E-value: 1.78e-114
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462538466 125 KALQKYMENLSKEYQTLEQCLQHIPV--NEENRRSLNRRHAELAPLAAIYQEIQETEQAIEELESMCKSlnkqDEKQLQE 202
Cdd:TIGR00019   3 PSLLEKLESLLERYEELEALLSDPEVisDQDKLRKLSKEYSQLEEIVDCYREYQQAQEDIKEAKEILEE----SDPEMRE 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462538466 203 LALEERQTIDQKINMLYNELFQSLVPKEKYDKNDVILEVTAGrtTGGDICQQFTREIFDMYQNYSCYKHWQFELLNYTPA 282
Cdd:TIGR00019  79 MAKEELEELEEKIEELEEQLKVLLLPKDPNDEKNVILEIRAG--TGGDEAAIFAGDLFRMYSRYAESKGWKVEILSANET 156

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462538466 283 DYGGLHHAAARISGDGVYKHLKYEGGIHRVQRIPEvglSSRMQRIHTGTMSVIVLPQPDEVDVKLDPKDLRIDTFRAKGA 362
Cdd:TIGR00019 157 ELGGYKEVIAEIKGDGVYSRLKFESGVHRVQRVPV---TESQGRIHTSAATVAVMPELEEVEVDINPADLRIDTFRSSGA 233

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462538466 363 GGQHVNKTDSAVRLVHIPTGLVVECQQERSQIKNKEIAFRVLRARLYQQIIEKDKRQQQSARKLQVGTRAQSERIRTYNF 442
Cdd:TIGR00019 234 GGQHVNTTDSAVRITHLPTGIVVECQDERSQHKNKDKAMKVLRARLYEAEQEKQQAAQASTRKSQVGSGDRSERIRTYNF 313

                         330       340       350       360
                  ....*....|....*....|....*....|....*....|....*....
gi 2462538466 443 TQDRVSDHRIAYEVRDIKEFLCGgkGLDQLIQRLLQSADEEAIAELLDE 491
Cdd:TIGR00019 314 PQNRVTDHRINLTLYKLDEVLEG--DLDELIEALIAEDQAQQLAALSEE 360
PCRF pfam03462
PCRF domain; This domain is found in peptide chain release factors.
 134-334 6.43e-65

PCRF domain; This domain is found in peptide chain release factors.


Pssm-ID: 460929 [Multi-domain]  Cd Length: 192  Bit Score: 208.39  E-value: 6.43e-65
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462538466 134 LSKEYQTLEQCLQHIPV--NEENRRSLNRRHAELAPLAAIYQEIQETEQAIEELESMCKslnkqdEKQLQELALEERQTI 211
Cdd:pfam03462   1 LEERYEELEALLSDPDVwdDQKRAQKLSKEYSELEPIVEAYREYKQALEDLEEAKELLE------DPELAELAEEELEEL 74

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462538466 212 DQKINMLYNELFQSLVPKEKYDKNDVILEVTAGrtTGGDICQQFTREIFDMYQNYSCYKHWQFELLNYTPADYGGLHHAA 291
Cdd:pfam03462  75 EKRLEELEEELKLLLLPKDPNDDKNAILEIRAG--AGGDEAALFAGDLFRMYTRYAERKGWKVEVLDYSETEAGGIKEAT 152

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|...
gi 2462538466 292 ARISGDGVYKHLKYEGGIHRVQRIPEvglSSRMQRIHTGTMSV 334
Cdd:pfam03462 153 FEISGEGAYGRLKFESGVHRVQRVPP---TESQGRIHTSFATV 192
PCRF smart00937
This domain is found in peptide chain release factors;
190-306 5.14e-35

This domain is found in peptide chain release factors;


Pssm-ID: 214923 [Multi-domain]  Cd Length: 116  Bit Score: 126.81  E-value: 5.14e-35
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462538466  190 KSLNKQDEKQLQELALEERQTI-DQKINMLYNELFQSLVPKEKYDKNDVILEVTAGrtTGGDICQQFTREIFDMYQNYSC 268
Cdd:smart00937   1 ELLEEEEDDEMRELAEEELEELeAELEEELEELLKLLLLPKDPNDDKNAILEIRAG--AGGDEAALFAGDLLRMYLRYAE 78

                           90       100       110
                   ....*....|....*....|....*....|....*...
gi 2462538466  269 YKHWQFELLNYTPADYGGLHHAAARISGDGVYKHLKYE 306
Cdd:smart00937  79 RKGWKVEVLDESEGELGGIKEATLKISGEGAYGKLKFE 116
 
Name Accession Description Interval E-value
PrfA COG0216
Protein chain release factor RF1 [Translation, ribosomal structure and biogenesis]; Protein ...
 131-488 1.47e-138

Protein chain release factor RF1 [Translation, ribosomal structure and biogenesis]; Protein chain release factor RF1 is part of the Pathway/BioSystem: Translation factors


Pssm-ID: 439986 [Multi-domain]  Cd Length: 356  Bit Score: 403.23  E-value: 1.47e-138
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462538466 131 MENLSKEYQTLEQCLQ--HIPVNEENRRSLNRRHAELAPLAAIYQEIQETEQAIEELESMcksLNKQDEKQLQELALEER 208
Cdd:COG0216     6 LEALEERYEELEALLSdpEVISDQKRFRKLSKEYAELEPIVEAYREYKKLLEDIEEAKEL---LEEESDPEMREMAKEEL 82

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462538466 209 QTIDQKINMLYNELFQSLVPKEKYDKNDVILEVTAGrtTGGD---IcqqFTREIFDMYQNYSCYKHWQFELLNYTPADYG 285
Cdd:COG0216    83 EELEARLEELEEELKILLLPKDPNDDKNVILEIRAG--TGGDeaaL---FAGDLFRMYSRYAERKGWKVEILSASEGELG 157

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462538466 286 GLHHAAARISGDGVYKHLKYEGGIHRVQRIPEV---GlssrmqRIHTGTMSVIVLPQPDEVDVKLDPKDLRIDTFRAKGA 362
Cdd:COG0216   158 GYKEVIFRISGEGVYSRLKFESGVHRVQRVPETesqG------RIHTSAATVAVLPEAEEVDVEINPKDLRIDTFRSSGA 231

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462538466 363 GGQHVNKTDSAVRLVHIPTGLVVECQQERSQIKNKEIAFRVLRARLYQQIIEKDKRQQQSARKLQVGTRAQSERIRTYNF 442
Cdd:COG0216   232 GGQHVNTTDSAVRITHLPTGIVVECQDERSQHKNKAKAMKVLRARLYEAEQQKQQAEIAAERKSQVGSGDRSERIRTYNF 311

                         330       340       350       360
                  ....*....|....*....|....*....|....*....|....*.
gi 2462538466 443 TQDRVSDHRIAYEVRDIKEFLCGgkGLDQLIQRLLQSADEEAIAEL 488
Cdd:COG0216   312 PQGRVTDHRINLTLYKLDEVMEG--DLDELIDALIAEDQAEKLAAL 355
prfA PRK00591
peptide chain release factor 1; Validated
 131-488 1.42e-135

peptide chain release factor 1; Validated


Pssm-ID: 234801 [Multi-domain]  Cd Length: 359  Bit Score: 395.60  E-value: 1.42e-135
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462538466 131 MENLSKEYQTLEQCLQ--HIPVNEENRRSLNRRHAELAPLAAIYQEIQETEQAIEELESMcksLNKQDEKQLQELALEER 208
Cdd:PRK00591    8 LEALEERYEELEALLSdpEVISDQKRFRKLSKEYAELEPIVEAYREYKQAQEDLEEAKEM---LEEESDPEMREMAKEEL 84

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462538466 209 QTIDQKINMLYNELFQSLVPKEKYDKNDVILEVTAGrtTGGDICQQFTREIFDMYQNYSCYKHWQFELLNYTPADYGGLH 288
Cdd:PRK00591   85 KELEERLEELEEELKILLLPKDPNDDKNVILEIRAG--TGGDEAALFAGDLFRMYSRYAERQGWKVEILSASEGELGGYK 162

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462538466 289 HAAARISGDGVYKHLKYEGGIHRVQRIPEV---GlssrmqRIHTGTMSVIVLPQPDEVDVKLDPKDLRIDTFRAKGAGGQ 365
Cdd:PRK00591  163 EVIAEISGDGVYSKLKFESGVHRVQRVPATesqG------RIHTSAATVAVLPEAEEVEVEINPKDLRIDTFRSSGAGGQ 236

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462538466 366 HVNKTDSAVRLVHIPTGLVVECQQERSQIKNKEIAFRVLRARLYQQIIEKDKRQQQSARKLQVGTRAQSERIRTYNFTQD 445
Cdd:PRK00591  237 HVNTTDSAVRITHLPTGIVVECQDERSQHKNKAKAMKVLRARLYDAERQKAQAEEAATRKSQVGSGDRSERIRTYNFPQG 316

                         330       340       350       360
                  ....*....|....*....|....*....|....*....|...
gi 2462538466 446 RVSDHRIAYEVRDIKEFLCGgkGLDQLIQRLLQSADEEAIAEL 488
Cdd:PRK00591  317 RVTDHRINLTLYKLDEVMEG--DLDELIDALIAEDQAEKLAAL 357
prfA TIGR00019
peptide chain release factor 1; This model describes peptide chain release factor 1 (PrfA, ...
 125-491 1.78e-114

peptide chain release factor 1; This model describes peptide chain release factor 1 (PrfA, RF-1), and excludes the related peptide chain release factor 2 (PrfB, RF-2). RF-1 helps recognize and terminate translation at UAA and UAG stop codons. The mitochondrial release factors are prfA-like, although not included above the trusted cutoff for this model. RF-1 does not have a translational frameshift. [Protein synthesis, Translation factors]


Pssm-ID: 129130 [Multi-domain]  Cd Length: 360  Bit Score: 342.06  E-value: 1.78e-114
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462538466 125 KALQKYMENLSKEYQTLEQCLQHIPV--NEENRRSLNRRHAELAPLAAIYQEIQETEQAIEELESMCKSlnkqDEKQLQE 202
Cdd:TIGR00019   3 PSLLEKLESLLERYEELEALLSDPEVisDQDKLRKLSKEYSQLEEIVDCYREYQQAQEDIKEAKEILEE----SDPEMRE 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462538466 203 LALEERQTIDQKINMLYNELFQSLVPKEKYDKNDVILEVTAGrtTGGDICQQFTREIFDMYQNYSCYKHWQFELLNYTPA 282
Cdd:TIGR00019  79 MAKEELEELEEKIEELEEQLKVLLLPKDPNDEKNVILEIRAG--TGGDEAAIFAGDLFRMYSRYAESKGWKVEILSANET 156

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462538466 283 DYGGLHHAAARISGDGVYKHLKYEGGIHRVQRIPEvglSSRMQRIHTGTMSVIVLPQPDEVDVKLDPKDLRIDTFRAKGA 362
Cdd:TIGR00019 157 ELGGYKEVIAEIKGDGVYSRLKFESGVHRVQRVPV---TESQGRIHTSAATVAVMPELEEVEVDINPADLRIDTFRSSGA 233

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462538466 363 GGQHVNKTDSAVRLVHIPTGLVVECQQERSQIKNKEIAFRVLRARLYQQIIEKDKRQQQSARKLQVGTRAQSERIRTYNF 442
Cdd:TIGR00019 234 GGQHVNTTDSAVRITHLPTGIVVECQDERSQHKNKDKAMKVLRARLYEAEQEKQQAAQASTRKSQVGSGDRSERIRTYNF 313

                         330       340       350       360
                  ....*....|....*....|....*....|....*....|....*....
gi 2462538466 443 TQDRVSDHRIAYEVRDIKEFLCGgkGLDQLIQRLLQSADEEAIAELLDE 491
Cdd:TIGR00019 314 PQNRVTDHRINLTLYKLDEVLEG--DLDELIEALIAEDQAQQLAALSEE 360
prfB TIGR00020
peptide chain release factor 2; In many but not all taxa, there is a conserved real ...
 164-479 2.82e-68

peptide chain release factor 2; In many but not all taxa, there is a conserved real translational frameshift at a TGA codon. RF-2 helps terminate translation at TGA codons and can therefore regulate its own production by readthrough when RF-2 is insufficient. There is a Pfam model called "RF-1" for the superfamily of RF-1, RF-2, mitochondrial, RF-H, etc. [Protein synthesis, Translation factors]


Pssm-ID: 272858 [Multi-domain]  Cd Length: 364  Bit Score: 223.11  E-value: 2.82e-68
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462538466 164 ELAPLAAIYQEIQETEQAIEELESMCKSLNKQDEKQLQELALEERQTIDQKINMLyneLFQSLVpKEKYDKNDVILEVTA 243
Cdd:TIGR00020  57 ERSSLEAVLDTLEELKNSLEDLSELLELAVEEDDEETFNELDAELKALEKKLAEL---ELRTML-SGEYDANNAYLTIQA 132

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462538466 244 GrtTGGDICQQFTREIFDMYQNYSCYKHWQFELLNYTPADYGGLHHAAARISGDGVYKHLKYEGGIHRVQRIPEVGLSSR 323
Cdd:TIGR00020 133 G--AGGTEAQDWASMLYRMYLRWAERRGFKVEIIDYSEGEEAGIKSVTILIKGPYAYGYLKSEQGVHRLVRISPFDANGR 210

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462538466 324 MqriHTGTMSVIVLPQ-PDEVDVKLDPKDLRIDTFRAKGAGGQHVNKTDSAVRLVHIPTGLVVECQQERSQIKNKEIAFR 402
Cdd:TIGR00020 211 R---HTSFASVFVMPEvDDDIDIEIKPEDLRIDTYRASGAGGQHVNKTDSAVRITHIPTGIVVQCQNDRSQHKNKDSAMK 287

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462538466 403 VLRARLYQQIIEKdkrqQQSARKLQVGTRAQS---ERIRTYNF-TQDRVSDHRIAYEVRDIKEFLCGgkGLDQLIQRLLQ 478
Cdd:TIGR00020 288 VLKAKLYELEMEK----EQAEKDAKEGEKSEIgwgSQIRSYVLhPYSMVKDLRTGYETGNVQAVLDG--DIDQFIEAYLK 361


                  .
gi 2462538466 479 S 479
Cdd:TIGR00020 362 W 362
PCRF pfam03462
PCRF domain; This domain is found in peptide chain release factors.
 134-334 6.43e-65

PCRF domain; This domain is found in peptide chain release factors.


Pssm-ID: 460929 [Multi-domain]  Cd Length: 192  Bit Score: 208.39  E-value: 6.43e-65
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462538466 134 LSKEYQTLEQCLQHIPV--NEENRRSLNRRHAELAPLAAIYQEIQETEQAIEELESMCKslnkqdEKQLQELALEERQTI 211
Cdd:pfam03462   1 LEERYEELEALLSDPDVwdDQKRAQKLSKEYSELEPIVEAYREYKQALEDLEEAKELLE------DPELAELAEEELEEL 74

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462538466 212 DQKINMLYNELFQSLVPKEKYDKNDVILEVTAGrtTGGDICQQFTREIFDMYQNYSCYKHWQFELLNYTPADYGGLHHAA 291
Cdd:pfam03462  75 EKRLEELEEELKLLLLPKDPNDDKNAILEIRAG--AGGDEAALFAGDLFRMYTRYAERKGWKVEVLDYSETEAGGIKEAT 152

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|...
gi 2462538466 292 ARISGDGVYKHLKYEGGIHRVQRIPEvglSSRMQRIHTGTMSV 334
Cdd:pfam03462 153 FEISGEGAYGRLKFESGVHRVQRVPP---TESQGRIHTSFATV 192
RF-1 pfam00472
RF-1 domain; This domain is found in peptide chain release factors such as RF-1 and RF-2, and ...
 341-451 2.17e-58

RF-1 domain; This domain is found in peptide chain release factors such as RF-1 and RF-2, and a number of smaller proteins of unknown function. This domain contains the peptidyl-tRNA hydrolase activity. The domain contains a highly conserved motif GGQ, where the glutamine is thought to coordinate the water that mediates the hydrolysis.


Pssm-ID: 459823 [Multi-domain]  Cd Length: 111  Bit Score: 188.40  E-value: 2.17e-58
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462538466 341 DEVDVKLDPKDLRIDTFRAKGAGGQHVNKTDSAVRLVHIPTGLVVECQQERSQIKNKEIAFRVLRARLYQqiIEKDKRQQ 420
Cdd:pfam00472   1 DEIEIEIDPKDLRIDTFRSSGAGGQHVNKTDSAVRLTHIPTGIVVKCQDERSQHQNREKALKRLRARLYE--LEEEKEEE 78

                          90       100       110
                  ....*....|....*....|....*....|...
gi 2462538466 421 QSAR-KLQVGTRAQSERIRTYNFT-QDRVSDHR 451
Cdd:pfam00472  79 KKRRiKSQVGSGDRSEQIRTYNFKpQSRVKDHR 111
PRK05589 PRK05589
peptide chain release factor 2; Provisional
 178-459 7.54e-53

peptide chain release factor 2; Provisional


Pssm-ID: 235520 [Multi-domain]  Cd Length: 325  Bit Score: 181.54  E-value: 7.54e-53
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462538466 178 TEQAIEELESMCKSLNKQDEKQLQELaLEERQTIDQKINMLYNELFQSlvpkEKYDKNDVILEVTAGrtTGGDICQQFTR 257
Cdd:PRK05589   33 LRNRIEDIEVLCEMMSEEDDEMKKEI-ISEVKNIKEEIDRFKIETLLS----GEYDRNNAILTLHSG--VGGTDAQDWTE 105

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462538466 258 EIFDMYQNYSCYKHWQFELLNYTPADYGGLHHAAARISGDGVYKHLKYEGGIHRVQRIPEVGLSSRMQrihTGTMSVIVL 337
Cdd:PRK05589  106 MLLRMYTRWAEKKGYKVEIIDLLEGDEAGIKSVTLKITGEFAYGYLKAEKGIHRLVRISPFNANGKRQ---TSFASVEVL 182

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462538466 338 PQ-PDEVDVKLDPKDLRIDTFRAKGAGGQHVNKTDSAVRLVHIPTGLVVECQQERSQIKNKEIAFRVLRARLYQ--QIIE 414
Cdd:PRK05589  183 PElTDDQDIEIRSEDLKIDTYRAGGAGGQHVNKTESAVRITHIPTGIVVQCQNERSQHSNKETAMKMLKSKLVElkERAH 262

                         250       260       270       280
                  ....*....|....*....|....*....|....*....|....*.
gi 2462538466 415 KDKRQQQSARKLQVGTRAQserIRTYNF-TQDRVSDHRIAYEVRDI 459
Cdd:PRK05589  263 KEKIEDLTGELKDMGWGSQ---IRSYVFhPYNLVKDHRTGVETSNV 305
PRK08787 PRK08787
peptide chain release factor 2; Provisional
 163-480 1.12e-50

peptide chain release factor 2; Provisional


Pssm-ID: 136970 [Multi-domain]  Cd Length: 313  Bit Score: 175.23  E-value: 1.12e-50
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462538466 163 AELAPLAAIYQEiQETEQAIeelesmcksLNKQDEKQLQELALEerqtidqkinmlYNELFQSlvpkeKYDKNDVILEVT 242
Cdd:PRK08787   20 GELLDLAESEQD-EDTALAV---------IADLDKYQAHVEKLE------------FQRMFSG-----QMDGANAFVDIQ 72

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462538466 243 AGrtTGGDICQQFTREIFDMYQNYSCYKHWQFELLNYTPADYGGLHHAAARISGDGVYKHLKYEGGIHRVQRIPEVGLSS 322
Cdd:PRK08787   73 AG--AGGTEAQDWAEILLRMYLRWAESRGWKTELMEVSGGEVAGIKSATVRIEGEYAYGWLKTEIGVHRLVRKSPFDSDN 150

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462538466 323 RMqriHTGTMSVIVLPQPDE-VDVKLDPKDLRIDTFRAKGAGGQHVNKTDSAVRLVHIPTGLVVECQQERSQIKNKEIAF 401
Cdd:PRK08787  151 RR---HTSFTSVFVSPEVDDnIEIDINPADLRTDVYRSSGAGGQHVNKTESAVRITHIPTNTVVACQTGRSQHQNRDNAM 227

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462538466 402 RVLRARLYQQIIEKDKRQQQS--ARKLQVGTRAQserIRTYNFTQDRVSDHRIAYEVRDIKEFLCGgkGLDQLIQRLLQS 479
Cdd:PRK08787  228 KMLAAKLYELEVQKRNAEKDAleATKSDIGWGSQ---IRNYVLDQSRIKDLRTGIERSDTQKVLDG--DLDEFVEASLKA 302


                  .
gi 2462538466 480 A 480
Cdd:PRK08787  303 G 303
PRK06746 PRK06746
peptide chain release factor 2; Provisional
 164-479 1.31e-48

peptide chain release factor 2; Provisional


Pssm-ID: 75726 [Multi-domain]  Cd Length: 326  Bit Score: 170.30  E-value: 1.31e-48
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462538466 164 ELAPLAAIYQEIQETEQAIEELESMCKSLNKQDEKQLQELALEERQTIDQKINmlynELFQSLVPKEKYDKNDVILEVTA 243
Cdd:PRK06746   19 EANALKDMVGKFRQLDETFENLEITHELLKEEYDEDLHEELESEVKGLIQEMN----EYELQLLLSDPYDKNNAILELHP 94

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462538466 244 GrtTGGDICQQFTREIFDMYQNYSCYKHWQFELLNYTPADYGGLHHAAARISGDGVYKHLKYEGGIHRVQRIPEVGLSSR 323
Cdd:PRK06746   95 G--AGGTESQDWGSMLLRMYTRWAEKRGFKVETVDYLPGDEAGIKSVTLLIKGHNAYGYLKAEKGVHRLVRISPFDSSGR 172

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462538466 324 MqriHTGTMSVIVLPQ-PDEVDVKLDPKDLRIDTFRAKGAGGQHVNKTDSAVRLVHIPTGLVVECQQERSQIKNKEIAFR 402
Cdd:PRK06746  173 R---HTSFVSCEVVPEfNDEVEIEVRTEDLKIDTYRASGAGGQHVNTTDSAVRITHTPTNTVVTCQSERSQIKNREHAMK 249

                         250       260       270       280       290       300       310       320
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462538466 403 VLRARLYQQIIEKDKRQQQSARKLQ--VGTRAQserIRTYNF-TQDRVSDHRIAYEVRDIKEFLCGgkGLDQLIQRLLQS 479
Cdd:PRK06746  250 MLKAKLYQKKLEEQQAELDEIRGEQkeIGWGSQ---IRSYVFhPYSLVKDHRTNTEVGNVQAVMDG--EIDPFIDAYLRS 324
PRK07342 PRK07342
peptide chain release factor 2; Provisional
 173-440 7.12e-45

peptide chain release factor 2; Provisional


Pssm-ID: 235997 [Multi-domain]  Cd Length: 339  Bit Score: 160.43  E-value: 7.12e-45
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462538466 173 QEIQETEQAIEELESMCKSLNkqDEKQLQELALEER-----QTIDQKINMLYNEL----FQSLVPKEKyDKNDVILEVTA 243
Cdd:PRK07342   20 RERQQLDDSINGINHLEQTLN--DNIELIAMGEEEGdksivEDAEKTIRDLKDEIdrrqIDALLSGEA-DANDTYLEVHA 96

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462538466 244 GrtTGGDICQQFTREIFDMYQNYSCYKHWQFELLNYTPADYGGLHHAAARISGDGVYKHLKYEGGIHRVQRIPEVGLSSR 323
Cdd:PRK07342   97 G--AGGTESQDWASMLLRMYTRWAERQGRKVEVLEVHDGEEAGIKSATILVKGHNAYGWLKTESGVHRLVRISPYDSNAR 174

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462538466 324 MqriHTGTMSVIVLPQPDE-VDVKLDPKDLRIDTFRAKGAGGQHVNKTDSAVRLVHIPTGLVVECQQERSQIKNKEIAFR 402
Cdd:PRK07342  175 R---HTSFASIWVYPVIDDnIEVDVNESDVRIDTYRSSGAGGQHVNTTDSAVRITHIPTGIVVQCQQERSQHKNRAKAWS 251

                         250       260       270       280
                  ....*....|....*....|....*....|....*....|
gi 2462538466 403 VLRARLYQQIIEK--DKRQQQSARKLQVGTRAQserIRTY 440
Cdd:PRK07342  252 MLRARLYEEELKKreEATNAAAASKTDIGWGHQ---IRSY 288
PCRF smart00937
This domain is found in peptide chain release factors;
190-306 5.14e-35

This domain is found in peptide chain release factors;


Pssm-ID: 214923 [Multi-domain]  Cd Length: 116  Bit Score: 126.81  E-value: 5.14e-35
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462538466  190 KSLNKQDEKQLQELALEERQTI-DQKINMLYNELFQSLVPKEKYDKNDVILEVTAGrtTGGDICQQFTREIFDMYQNYSC 268
Cdd:smart00937   1 ELLEEEEDDEMRELAEEELEELeAELEEELEELLKLLLLPKDPNDDKNAILEIRAG--AGGDEAALFAGDLLRMYLRYAE 78

                           90       100       110
                   ....*....|....*....|....*....|....*...
gi 2462538466  269 YKHWQFELLNYTPADYGGLHHAAARISGDGVYKHLKYE 306
Cdd:smart00937  79 RKGWKVEVLDESEGELGGIKEATLKISGEGAYGKLKFE 116
PrfB COG1186
Protein chain release factor PrfB [Translation, ribosomal structure and biogenesis]; Protein ...
 344-451 2.31e-33

Protein chain release factor PrfB [Translation, ribosomal structure and biogenesis]; Protein chain release factor PrfB is part of the Pathway/BioSystem: Translation factors


Pssm-ID: 440799 [Multi-domain]  Cd Length: 140  Bit Score: 123.32  E-value: 2.31e-33
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462538466 344 DVKLDPKDLRIDTFRAKGAGGQHVNKTDSAVRLVH------------IPTGLVVECQQERSQIKNKEIAFRVLRARLYQQ 411
Cdd:COG1186    20 EIEIPESDLRIDTYRASGAGGQNVNKVSSAVRLTHdiassslpdsrlTPGGIVVKAQEERSQHQNREDALKRLKALLYEA 99

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|
gi 2462538466 412 IIEKDKRQQQSARKLQVGTRAQSERIRTynftqdRVSDHR 451
Cdd:COG1186   100 ELEPKERRATKPTRGSKKRRLWGKQIRS------MVKDLR 133
release_prfH TIGR03072
putative peptide chain release factor H; Members of this protein family are bacterial proteins ...
 349-424 1.07e-20

putative peptide chain release factor H; Members of this protein family are bacterial proteins homologous to peptide chain release factors 1 (RF-1, product of the prfA gene), and 2 (RF-2, product of the prfB gene). The member from Escherichia coli K-12, designated prfH, appears to be a pseudogene. This class I release factor is always found as the downstream gene of a two-gene operon. [Protein synthesis, Translation factors]


Pssm-ID: 213768  Cd Length: 200  Bit Score: 89.83  E-value: 1.07e-20
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 2462538466 349 PKDLRIDTFRAKGAGGQHVNKTDSAVRLVHIPTGLVVECQQERSQIKNKEIAFRVLRARLYQQIIEKDKRQQQSAR 424
Cdd:TIGR03072 105 EDEIRFETLRSSGPGGQHVNKTESAVRATHLASGISVKVQSERSQHANKRLATLLLAVRLADLQQEQAAALRAERR 180
PRK09256 PRK09256
aminoacyl-tRNA hydrolase;
344-408 5.32e-09

aminoacyl-tRNA hydrolase;


Pssm-ID: 181730 [Multi-domain]  Cd Length: 138  Bit Score: 54.77  E-value: 5.32e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 2462538466 344 DVKLDPKDLRIDTFRAKGAGGQHVNKTDSAVRLV------HIP---------------TG---LVVECQQERSQIKNKEI 399
Cdd:PRK09256    7 RLVIPENELEWRFIRASGPGGQNVNKVSTAVELRfdiaasSLPefykerllalaghriTKdgvIVIKAQEFRSQERNRED 86


                  ....*....
gi 2462538466 400 AfrvlRARL 408
Cdd:PRK09256   87 A----LERL 91
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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