Putative GlcNAc-1 phosphotransferase regulatory domain; The Golgi enzyme UDP-GlcNAc-lysosomal enzyme N-acetylglucosamine-1-phosphotransferase (GlcNAc-1-phosphotransferase), an alpha2beta2gamma2 hexamer, mediates the initial step in the addition of the mannose 6-phosphate targeting signal on newly synthesized lysosomal enzymes. GNPTAB encodes the alpha and beta subunits of GlcNAc-1-phosphotransferase, and mutations in this gene cause the lysosomal storage disorders mucolipidosis II and III alpha-beta The alpha-beta subunits contain three identifiable domains separated by so-called spacer regions. This domain is part of the first spacer region, Spacer-1. Studies indicate that GlcNAc-1 lacking spacer-1 exhibits enhanced phosphorylation of several non-lysosomal glycoproteins, while the phosphorylation of lysosomal acid hydrolases is not altered. In view of these effects on the maturation and function of GlcNAc-1, it is suggested to rename 'spacer-1' the 'regulatory-1' domain.

                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 84579827    219 PGLVLMQGLAFLSGFPPTFKETSQLKTKLPEKLSSKIKLLRLYSEASVALLKLNNPKGFQELNKQTKKNMTIDGKELTIS 298
Cdd:pfam18440    2 PGLVLMQDLAFLSGFPRTFKETNQLKTKLPEKLRSKVKLLQLYSEASVALLDLNNPKDFDELLKQGKKNMTIDGKELTLT 81

                   ....*..
gi 84579827    299 PAYLLWD 305
Cdd:pfam18440   82 PAYLLWD 88

DMAP1-binding Domain; This domain binds DMAP1, a transcriptional co-repressor.

                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 84579827    699 NISLLPKEAQVRLSNLDLQLERGDITLKGYNLSKSALLRSFLGNSLDTkikpqartdeTKGNLEVPQENPSH--RRPHGf 776
Cdd:pfam06464    1 NPPSLPDDVRERLSELDLDLSEGDITEKGYEKKKLKLLRKFLLHPETP----------TKLSAEAQNQLASLetKLRDE- 69

                           90       100       110
                   ....*....|....*....|....*....|....*
gi 84579827    777 AGEHRSERWTAPAETVTVKGRDHALNPPPVLETNA 811
Cdd:pfam06464   70 ELSEEVYLEKVKALLAKELERENGLNAPTKEQSGL 104

Stealth protein CR3, conserved region 3; Stealth_CR3 is the third of several highly conserved regions on stealth proteins in metazoa and bacteria. There are up to four CR regions on all member proteins. The domain is found in tandem with CR1, CR2 and CR4 on both potential metazoan hosts and pathogenic eubacterial species that are capsular polysaccharide phosphotransferases. The CR domains appear on eukaryotic proteins such as GNPTAB, N-acetylglucosamine-1-phosphotransferase subunits alpha/beta. Horizontal gene-transfer seems to have occurred between host and bacteria of these sequence-regions in order for the bacteria to evade detection by the host innate immune system.

                           10        20        30        40
                   ....*....|....*....|....*....|....*....|....*....
gi 84579827    934 AHMPHMIDRIVMQELQDMFPEEFDKTSFHKVRHSEDMQFAFSYFYYLMS 982
Cdd:pfam17102    1 AHTPHPLRRSVLEEIEERFPEEFARTASHRFRSPDDLSMAFSYHHYYAE 49

Stealth protein CR1, conserved region 1; Stealth_C1 is the first of several highly conserved regions on stealth proteins in metazoa and bacteria. There are up to four CR regions on all member proteins. CR1 carries a well-conserved IDVVYT sequence-motif. The domain is found in tandem with CR2, CR3 and CR4 on both potential metazoan hosts and pathogenic eubacterial species that are capsular polysaccharide phosphotransferases. The CR domains appear on eukaryotic proteins such as GNPTAB, N-acetylglucosamine-1-phosphotransferase subunits alpha/beta. Horizontal gene-transfer seems to have occurred between host and bacteria of these sequence-regions in order for the bacteria to evade detection by the host innate immune system.

                           10        20
                   ....*....|....*....|....*....
gi 84579827     73 MPIDVVYTWVNGTDLELLKELQQVREHME 101
Cdd:pfam17101    1 FPIDVVYTWVNGSDPEWQAKKAKYLGKLG 29

Putative GlcNAc-1 phosphotransferase regulatory domain; The Golgi enzyme UDP-GlcNAc-lysosomal enzyme N-acetylglucosamine-1-phosphotransferase (GlcNAc-1-phosphotransferase), an alpha2beta2gamma2 hexamer, mediates the initial step in the addition of the mannose 6-phosphate targeting signal on newly synthesized lysosomal enzymes. GNPTAB encodes the alpha and beta subunits of GlcNAc-1-phosphotransferase, and mutations in this gene cause the lysosomal storage disorders mucolipidosis II and III alpha-beta The alpha-beta subunits contain three identifiable domains separated by so-called spacer regions. This domain is part of the first spacer region, Spacer-1. Studies indicate that GlcNAc-1 lacking spacer-1 exhibits enhanced phosphorylation of several non-lysosomal glycoproteins, while the phosphorylation of lysosomal acid hydrolases is not altered. In view of these effects on the maturation and function of GlcNAc-1, it is suggested to rename 'spacer-1' the 'regulatory-1' domain.

                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 84579827    219 PGLVLMQGLAFLSGFPPTFKETSQLKTKLPEKLSSKIKLLRLYSEASVALLKLNNPKGFQELNKQTKKNMTIDGKELTIS 298
Cdd:pfam18440    2 PGLVLMQDLAFLSGFPRTFKETNQLKTKLPEKLRSKVKLLQLYSEASVALLDLNNPKDFDELLKQGKKNMTIDGKELTLT 81

                   ....*..
gi 84579827    299 PAYLLWD 305
Cdd:pfam18440   82 PAYLLWD 88

RNA recognition motif 2 (RRM2) found in N-acetylglucosamine-1-phosphotransferase subunits alpha/beta (GNPTAB) and similar proteins; GNPTAB, also termed GlcNAc-1-phosphotransferase subunits alpha/beta, or stealth protein GNPTAB, or UDP-N-acetylglucosamine-1-phosphotransferase subunits alpha/beta, catalyzes the formation of mannose 6-phosphate (M6P) markers on high mannose type oligosaccharides in the Golgi apparatus. M6P residues are required to bind to the M6P receptors (MPR), which mediate the vesicular transport of lysosomal enzymes to the endosomal/prelysosomal compartment. The model corresponds to the RNA recognition motif 2 (RRM2) of GNPTAB. Its functional significance remains to be investigated.

                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 84579827  227 LAFLSGFPPTFkETSQLKTKLPEKLSSKIKLLRLYSEASVALLKLNNPKGFQELNKQTkkNMTIDGKELTISPAYLLWD 305
Cdd:cd21600    2 TIMMSGIPSTY-EEEQLLAKLPETIRSKISKVELYSEKGVAVLTLSDKKAFDELLKLS--NMTIDGKEITLSPAYLVWD 77

Stealth protein CR4, conserved region 4; Stealth_CR4 is the fourth highly conserved region on stealth proteins in metazoa and bacteria. There are four CR regions on mammalian members. CR4 carries a well-conserved CLND sequence-motif. The domain is found in tandem with CR1, CR2 and CR3 on both potential metazoan hosts and on pathogenic eubacterial species that are capsular polysaccharide phosphotransferases. The CR domains also appear on eukaryotic proteins such as GNPTAB, N-acetylglucosamine-1-phosphotransferase subunits alpha/beta. Horizontal gene-transfer seems to have occurred between host and bacteria of these sequence-regions in order for the bacteria to evade detection by the host innate immune system.

                           10        20        30        40        50
                   ....*....|....*....|....*....|....*....|....*....|....*..
gi 84579827   1117 DDIRKNPRKFVCLNDNIDHNHKDArtVKAVLRDFYESMFPIPSQFELPREYRNRFLH 1173
Cdd:pfam17103    1 DGIRRRRKKFFCLNDNIDPEVEEE--VKALLQDFLESLFPFPSPFELPPAYRNRFLY 55

LNR domain; The LNR (Lin-12/Notch repeat) domain is found in three tandem copies in Notch related proteins. The structure of the domain has been determined by NMR and was shown to contain three disulphide bonds and coordinate a calcium ion. Three repeats are also found in the PAPP-A peptidase.

                           10        20        30
                   ....*....|....*....|....*....|....*
gi 84579827    436 PNC-AEGCPgSWIKDGYCDKACNNSACDWDGGDCS 469
Cdd:pfam00066    2 PNCpYPYCW-DKFGNGVCDEECNNAECLWDGGDCS 35

Stealth protein CR1, conserved region 1; Stealth_C1 is the first of several highly conserved regions on stealth proteins in metazoa and bacteria. There are up to four CR regions on all member proteins. CR1 carries a well-conserved IDVVYT sequence-motif. The domain is found in tandem with CR2, CR3 and CR4 on both potential metazoan hosts and pathogenic eubacterial species that are capsular polysaccharide phosphotransferases. The CR domains appear on eukaryotic proteins such as GNPTAB, N-acetylglucosamine-1-phosphotransferase subunits alpha/beta. Horizontal gene-transfer seems to have occurred between host and bacteria of these sequence-regions in order for the bacteria to evade detection by the host innate immune system.

                           10        20
                   ....*....|....*....|....*....
gi 84579827     73 MPIDVVYTWVNGTDLELLKELQQVREHME 101
Cdd:pfam17101    1 FPIDVVYTWVNGSDPEWQAKKAKYLGKLG 29

Domain found in Notch and Lin-12; The Notch protein is essential for the proper differentiation of the Drosophila ectoderm. This protein contains 3 NL domains.

                            10        20
                    ....*....|....*....|.
gi 84579827     515 ADKFCDQACNVLSCGFDAGDC 535
Cdd:smart00004   18 GDGVCDEECNNAECLWDGGDC 38

Putative GlcNAc-1 phosphotransferase regulatory domain; The Golgi enzyme UDP-GlcNAc-lysosomal enzyme N-acetylglucosamine-1-phosphotransferase (GlcNAc-1-phosphotransferase), an alpha2beta2gamma2 hexamer, mediates the initial step in the addition of the mannose 6-phosphate targeting signal on newly synthesized lysosomal enzymes. GNPTAB encodes the alpha and beta subunits of GlcNAc-1-phosphotransferase, and mutations in this gene cause the lysosomal storage disorders mucolipidosis II and III alpha-beta The alpha-beta subunits contain three identifiable domains separated by so-called spacer regions. This domain is part of the first spacer region, Spacer-1. Studies indicate that GlcNAc-1 lacking spacer-1 exhibits enhanced phosphorylation of several non-lysosomal glycoproteins, while the phosphorylation of lysosomal acid hydrolases is not altered. In view of these effects on the maturation and function of GlcNAc-1, it is suggested to rename 'spacer-1' the 'regulatory-1' domain.

                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 84579827    219 PGLVLMQGLAFLSGFPPTFKETSQLKTKLPEKLSSKIKLLRLYSEASVALLKLNNPKGFQELNKQTKKNMTIDGKELTIS 298
Cdd:pfam18440    2 PGLVLMQDLAFLSGFPRTFKETNQLKTKLPEKLRSKVKLLQLYSEASVALLDLNNPKDFDELLKQGKKNMTIDGKELTLT 81

                   ....*..
gi 84579827    299 PAYLLWD 305
Cdd:pfam18440   82 PAYLLWD 88

RNA recognition motif 2 (RRM2) found in N-acetylglucosamine-1-phosphotransferase subunits alpha/beta (GNPTAB) and similar proteins; GNPTAB, also termed GlcNAc-1-phosphotransferase subunits alpha/beta, or stealth protein GNPTAB, or UDP-N-acetylglucosamine-1-phosphotransferase subunits alpha/beta, catalyzes the formation of mannose 6-phosphate (M6P) markers on high mannose type oligosaccharides in the Golgi apparatus. M6P residues are required to bind to the M6P receptors (MPR), which mediate the vesicular transport of lysosomal enzymes to the endosomal/prelysosomal compartment. The model corresponds to the RNA recognition motif 2 (RRM2) of GNPTAB. Its functional significance remains to be investigated.

                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 84579827  227 LAFLSGFPPTFkETSQLKTKLPEKLSSKIKLLRLYSEASVALLKLNNPKGFQELNKQTkkNMTIDGKELTISPAYLLWD 305
Cdd:cd21600    2 TIMMSGIPSTY-EEEQLLAKLPETIRSKISKVELYSEKGVAVLTLSDKKAFDELLKLS--NMTIDGKEITLSPAYLVWD 77

Stealth protein CR4, conserved region 4; Stealth_CR4 is the fourth highly conserved region on stealth proteins in metazoa and bacteria. There are four CR regions on mammalian members. CR4 carries a well-conserved CLND sequence-motif. The domain is found in tandem with CR1, CR2 and CR3 on both potential metazoan hosts and on pathogenic eubacterial species that are capsular polysaccharide phosphotransferases. The CR domains also appear on eukaryotic proteins such as GNPTAB, N-acetylglucosamine-1-phosphotransferase subunits alpha/beta. Horizontal gene-transfer seems to have occurred between host and bacteria of these sequence-regions in order for the bacteria to evade detection by the host innate immune system.

                           10        20        30        40        50
                   ....*....|....*....|....*....|....*....|....*....|....*..
gi 84579827   1117 DDIRKNPRKFVCLNDNIDHNHKDArtVKAVLRDFYESMFPIPSQFELPREYRNRFLH 1173
Cdd:pfam17103    1 DGIRRRRKKFFCLNDNIDPEVEEE--VKALLQDFLESLFPFPSPFELPPAYRNRFLY 55

LNR domain; The LNR (Lin-12/Notch repeat) domain is found in three tandem copies in Notch related proteins. The structure of the domain has been determined by NMR and was shown to contain three disulphide bonds and coordinate a calcium ion. Three repeats are also found in the PAPP-A peptidase.

                           10        20        30
                   ....*....|....*....|....*....|....*
gi 84579827    436 PNC-AEGCPgSWIKDGYCDKACNNSACDWDGGDCS 469
Cdd:pfam00066    2 PNCpYPYCW-DKFGNGVCDEECNNAECLWDGGDCS 35

Stealth protein CR1, conserved region 1; Stealth_C1 is the first of several highly conserved regions on stealth proteins in metazoa and bacteria. There are up to four CR regions on all member proteins. CR1 carries a well-conserved IDVVYT sequence-motif. The domain is found in tandem with CR2, CR3 and CR4 on both potential metazoan hosts and pathogenic eubacterial species that are capsular polysaccharide phosphotransferases. The CR domains appear on eukaryotic proteins such as GNPTAB, N-acetylglucosamine-1-phosphotransferase subunits alpha/beta. Horizontal gene-transfer seems to have occurred between host and bacteria of these sequence-regions in order for the bacteria to evade detection by the host innate immune system.

                           10        20
                   ....*....|....*....|....*....
gi 84579827     73 MPIDVVYTWVNGTDLELLKELQQVREHME 101
Cdd:pfam17101    1 FPIDVVYTWVNGSDPEWQAKKAKYLGKLG 29

Domain found in Notch and Lin-12; The Notch protein is essential for the proper differentiation of the Drosophila ectoderm. This protein contains 3 NL domains.

                            10        20
                    ....*....|....*....|.
gi 84579827     515 ADKFCDQACNVLSCGFDAGDC 535
Cdd:smart00004   18 GDGVCDEECNNAECLWDGGDC 38