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Conserved domains on  [gi|57527007|ref|NP_001009654|]
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kinetochore protein Spc25 [Rattus norvegicus]

Protein Classification

kinetochore Spc25 family protein( domain architecture ID 10548843)

kinetochore Spc25 family protein may be a component of the NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
RWD_Spc25 cd23784
RWD domain of kinetochore protein Spc25 and related proteins; Spc25 is a component of the ...
 130-217 7.93e-29

RWD domain of kinetochore protein Spc25 and related proteins; Spc25 is a component of the nuclear division cycle 80 (NDC80) kinetochore complex, which is essential for the stable formation of kinetochore-microtubule anchoring and correct chromosome segregation during mitosis. NDC80 has two subcomplexes, NDC80/NUF2 which binds directly to microtubules, and Spc24/Spc25 which interacts with the kinetochore. The inner kinetochore centromere protein T (CENP-T) has been shown to interact directly with the Spc24/25 RWD domains; these RWD domains serve as interaction modules at the kinetochores.


:

Pssm-ID: 467646  Cd Length: 87  Bit Score: 103.82  E-value: 7.93e-29
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 57527007 130 LKRLQKSADLYRDRLGLEIRKIPGDKLQFIFTSIDPKNPESPFMFSLSINEaKEYEVSDCSPHLECLAEFQEKVRKTNNF 209
Cdd:cd23784   1 LSELEKELELYEKLLGLRIEKTKDDRLKFVFTNIDPKDPEREFSFVLDVSE-DDYKVVECDPPLEDLDELLEELNETRDF 79


                ....*...
gi 57527007 210 SAFLANVR 217
Cdd:cd23784  80 SAFLKRMR 87
CwlO1 super family cl25603
Uncharacterized N-terminal coiled-coil domain of peptidoglycan hydrolase CwlO [Function ...
 69-165 7.72e-03

Uncharacterized N-terminal coiled-coil domain of peptidoglycan hydrolase CwlO [Function unknown];


The actual alignment was detected with superfamily member COG3883:

Pssm-ID: 443091 [Multi-domain]  Cd Length: 379  Bit Score: 36.73  E-value: 7.72e-03
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 57527007  69 QLCKQNKLIQEKKDNVLKMIAEVKGKGQEVEVLTANIQDLKEEYARKKETIstanKANEERLKRLQKSADLYRDRLGLEI 148
Cdd:COG3883  17 QIQAKQKELSELQAELEAAQAELDALQAELEELNEEYNELQAELEALQAEI----DKLQAEIAEAEAEIEERREELGERA 92

                        90       100
                ....*....|....*....|..
gi 57527007 149 RKI-----PGDKLQFIFTSIDP 165
Cdd:COG3883  93 RALyrsggSVSYLDVLLGSESF 114
 
Name Accession Description Interval E-value
RWD_Spc25 cd23784
RWD domain of kinetochore protein Spc25 and related proteins; Spc25 is a component of the ...
 130-217 7.93e-29

RWD domain of kinetochore protein Spc25 and related proteins; Spc25 is a component of the nuclear division cycle 80 (NDC80) kinetochore complex, which is essential for the stable formation of kinetochore-microtubule anchoring and correct chromosome segregation during mitosis. NDC80 has two subcomplexes, NDC80/NUF2 which binds directly to microtubules, and Spc24/Spc25 which interacts with the kinetochore. The inner kinetochore centromere protein T (CENP-T) has been shown to interact directly with the Spc24/25 RWD domains; these RWD domains serve as interaction modules at the kinetochores.


Pssm-ID: 467646  Cd Length: 87  Bit Score: 103.82  E-value: 7.93e-29
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 57527007 130 LKRLQKSADLYRDRLGLEIRKIPGDKLQFIFTSIDPKNPESPFMFSLSINEaKEYEVSDCSPHLECLAEFQEKVRKTNNF 209
Cdd:cd23784   1 LSELEKELELYEKLLGLRIEKTKDDRLKFVFTNIDPKDPEREFSFVLDVSE-DDYKVVECDPPLEDLDELLEELNETRDF 79


                ....*...
gi 57527007 210 SAFLANVR 217
Cdd:cd23784  80 SAFLKRMR 87
Spindle_Spc25 pfam08234
Chromosome segregation protein Spc25; This is a family of chromosome segregation proteins. It ...
 153-220 1.51e-20

Chromosome segregation protein Spc25; This is a family of chromosome segregation proteins. It contains Spc25, which is a conserved eukaryotic kinetochore protein involved in cell division. In fungi the Spc25 protein is a subunit of the Nuf2-Ndc80 complex, and in vertebrates it forms part of the Ndc80 complex.


Pssm-ID: 462402  Cd Length: 71  Bit Score: 81.86  E-value: 1.51e-20
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 57527007   153 GDKLQFIFTSIDPKNPESPFMFSLSINEaKEYEVSDCSPHLEC--LAEFQEKVRKTNNFSAFLANVRKAF 220
Cdd:pfam08234   3 GDKLKFVFTNIDPKDPEREFSFTLDVSE-DTYEVLECDPKLEDedLEELLDELNETNDLFKFLKRMRKAF 71
CwlO1 COG3883
Uncharacterized N-terminal coiled-coil domain of peptidoglycan hydrolase CwlO [Function ...
 69-165 7.72e-03

Uncharacterized N-terminal coiled-coil domain of peptidoglycan hydrolase CwlO [Function unknown];


Pssm-ID: 443091 [Multi-domain]  Cd Length: 379  Bit Score: 36.73  E-value: 7.72e-03
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 57527007  69 QLCKQNKLIQEKKDNVLKMIAEVKGKGQEVEVLTANIQDLKEEYARKKETIstanKANEERLKRLQKSADLYRDRLGLEI 148
Cdd:COG3883  17 QIQAKQKELSELQAELEAAQAELDALQAELEELNEEYNELQAELEALQAEI----DKLQAEIAEAEAEIEERREELGERA 92

                        90       100
                ....*....|....*....|..
gi 57527007 149 RKI-----PGDKLQFIFTSIDP 165
Cdd:COG3883  93 RALyrsggSVSYLDVLLGSESF 114
 
Name Accession Description Interval E-value
RWD_Spc25 cd23784
RWD domain of kinetochore protein Spc25 and related proteins; Spc25 is a component of the ...
 130-217 7.93e-29

RWD domain of kinetochore protein Spc25 and related proteins; Spc25 is a component of the nuclear division cycle 80 (NDC80) kinetochore complex, which is essential for the stable formation of kinetochore-microtubule anchoring and correct chromosome segregation during mitosis. NDC80 has two subcomplexes, NDC80/NUF2 which binds directly to microtubules, and Spc24/Spc25 which interacts with the kinetochore. The inner kinetochore centromere protein T (CENP-T) has been shown to interact directly with the Spc24/25 RWD domains; these RWD domains serve as interaction modules at the kinetochores.


Pssm-ID: 467646  Cd Length: 87  Bit Score: 103.82  E-value: 7.93e-29
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 57527007 130 LKRLQKSADLYRDRLGLEIRKIPGDKLQFIFTSIDPKNPESPFMFSLSINEaKEYEVSDCSPHLECLAEFQEKVRKTNNF 209
Cdd:cd23784   1 LSELEKELELYEKLLGLRIEKTKDDRLKFVFTNIDPKDPEREFSFVLDVSE-DDYKVVECDPPLEDLDELLEELNETRDF 79


                ....*...
gi 57527007 210 SAFLANVR 217
Cdd:cd23784  80 SAFLKRMR 87
Spindle_Spc25 pfam08234
Chromosome segregation protein Spc25; This is a family of chromosome segregation proteins. It ...
 153-220 1.51e-20

Chromosome segregation protein Spc25; This is a family of chromosome segregation proteins. It contains Spc25, which is a conserved eukaryotic kinetochore protein involved in cell division. In fungi the Spc25 protein is a subunit of the Nuf2-Ndc80 complex, and in vertebrates it forms part of the Ndc80 complex.


Pssm-ID: 462402  Cd Length: 71  Bit Score: 81.86  E-value: 1.51e-20
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 57527007   153 GDKLQFIFTSIDPKNPESPFMFSLSINEaKEYEVSDCSPHLEC--LAEFQEKVRKTNNFSAFLANVRKAF 220
Cdd:pfam08234   3 GDKLKFVFTNIDPKDPEREFSFTLDVSE-DTYEVLECDPKLEDedLEELLDELNETNDLFKFLKRMRKAF 71
CwlO1 COG3883
Uncharacterized N-terminal coiled-coil domain of peptidoglycan hydrolase CwlO [Function ...
 69-165 7.72e-03

Uncharacterized N-terminal coiled-coil domain of peptidoglycan hydrolase CwlO [Function unknown];


Pssm-ID: 443091 [Multi-domain]  Cd Length: 379  Bit Score: 36.73  E-value: 7.72e-03
                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 57527007  69 QLCKQNKLIQEKKDNVLKMIAEVKGKGQEVEVLTANIQDLKEEYARKKETIstanKANEERLKRLQKSADLYRDRLGLEI 148
Cdd:COG3883  17 QIQAKQKELSELQAELEAAQAELDALQAELEELNEEYNELQAELEALQAEI----DKLQAEIAEAEAEIEERREELGERA 92

                        90       100
                ....*....|....*....|..
gi 57527007 149 RKI-----PGDKLQFIFTSIDP 165
Cdd:COG3883  93 RALyrsggSVSYLDVLLGSESF 114
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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