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Conserved domains on  [gi|124486662|ref|NP_001074510|]
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vacuolar protein sorting-associated protein 51 homolog [Mus musculus]

Protein Classification

vacuolar protein sorting-associated protein 51 homolog( domain architecture ID 10554796)

vacuolar protein sorting-associated protein 51 homolog (VPS51) acts as component of the GARP complex that is involved in retrograde transport from early and late endosomes to the trans-Golgi network (TGN)

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Vps51 pfam08700
Vps51/Vps67; This family includes a presumed domain found in a number of components of ...
 63-147 4.94e-23

Vps51/Vps67; This family includes a presumed domain found in a number of components of vesicular transport. The VFT tethering complex (also known as GARP complex, Golgi associated retrograde protein complex, Vps53 tethering complex) is a conserved eukaryotic docking complex which is involved recycling of proteins from endosomes to the late Golgi. Vps51 (also known as Vps67) is a subunit of VFT and interacts with the SNARE Tlg1. Cog1_N is the N-terminus of the Cog1 subunit of the eight-unit Conserved Oligomeric Golgi (COG) complex that participates in retrograde vesicular transport and is required to maintain normal Golgi structure and function. The subunits are located in two lobes and Cog1 serves to bind the two lobes together probably via the highly conserved N-terminal domain of approximately 85 residues.


:

Pssm-ID: 462568  Cd Length: 86  Bit Score: 93.50  E-value: 4.94e-23
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124486662   63 LNGAHFDPEVYLDKLRRECPLAQLMDSETDMVRQIRALDSDMQTLVYENYNKFISATDTIRKMKNDFRKMEDEMDRLATN 142
Cdd:pfam08700   1 LDSPSFDADRYVSELLSKATLEELLQFESSLRSEIERLDSELKQLVYDNYRDLIKAADTISKMKSEMEQLSQKLSELKQA 80


                  ....*
gi 124486662  143 MAVIT 147
Cdd:pfam08700  81 LSKIA 85
COG2 super family cl19297
COG (conserved oligomeric Golgi) complex component, COG2; The COG complex comprises eight ...
 79-242 4.83e-10

COG (conserved oligomeric Golgi) complex component, COG2; The COG complex comprises eight proteins COG1-8. The COG complex plays critical roles in Golgi structure and function. The proposed function of the complex is to mediate the initial physical contact between transport vesicles and their membrane targets. A comparable role in tethering vesicles has been suggested for at least six additional large multisubunit complexes, including the exocyst, a complex that mediates trafficking to the plasma membrane. COG2 structure reveals a six-helix bundle with few conserved surface features but a general resemblance to recently determined crystal structures of four different exocyst subunits. These bundles inCOG2 may act as platforms for interaction with other trafficing proteins including SNAREs (soluble N-ethylmaleimide factor attachment protein receptors) and Rabs.


The actual alignment was detected with superfamily member pfam04124:

Pssm-ID: 473160  Cd Length: 315  Bit Score: 61.67  E-value: 4.83e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124486662   79 RECPLAQLMDSETDMVRQIRALDSDMQTLVYENYNKFISATDTIRKMKNDFRKMEDEMDRL---ATNMAV-ITNFSARiS 154
Cdd:pfam04124   5 STKPLEELEKEPELLAEEAAEILAQIQDLARKNYKTFIDAARATIAIRQLLGDIAKSLEQLvddIPKLTSsCVRFLED-A 83

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124486662  155 ATLQDRHERITKLAGVHAllrKLQFLFELPSRLTKCVELGAYGQAVRYQGRARAVLQQYQHLPSFRAIQDDCQVITARLA 234
Cdd:pfam04124  84 KEISETRRLNNLLLQNHD---QLLDLLELPQLMDTCIRTGYYSEALELAAYATRLRSRFPGSPVIQEIAAQVEEAMQTML 160


                  ....*...
gi 124486662  235 QQLRQRFR 242
Cdd:pfam04124 161 SQLIQQLR 168
 
Name Accession Description Interval E-value
Vps51 pfam08700
Vps51/Vps67; This family includes a presumed domain found in a number of components of ...
 63-147 4.94e-23

Vps51/Vps67; This family includes a presumed domain found in a number of components of vesicular transport. The VFT tethering complex (also known as GARP complex, Golgi associated retrograde protein complex, Vps53 tethering complex) is a conserved eukaryotic docking complex which is involved recycling of proteins from endosomes to the late Golgi. Vps51 (also known as Vps67) is a subunit of VFT and interacts with the SNARE Tlg1. Cog1_N is the N-terminus of the Cog1 subunit of the eight-unit Conserved Oligomeric Golgi (COG) complex that participates in retrograde vesicular transport and is required to maintain normal Golgi structure and function. The subunits are located in two lobes and Cog1 serves to bind the two lobes together probably via the highly conserved N-terminal domain of approximately 85 residues.


Pssm-ID: 462568  Cd Length: 86  Bit Score: 93.50  E-value: 4.94e-23
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124486662   63 LNGAHFDPEVYLDKLRRECPLAQLMDSETDMVRQIRALDSDMQTLVYENYNKFISATDTIRKMKNDFRKMEDEMDRLATN 142
Cdd:pfam08700   1 LDSPSFDADRYVSELLSKATLEELLQFESSLRSEIERLDSELKQLVYDNYRDLIKAADTISKMKSEMEQLSQKLSELKQA 80


                  ....*
gi 124486662  143 MAVIT 147
Cdd:pfam08700  81 LSKIA 85
Dor1 pfam04124
Dor1-like family; Dor1 is involved in vesicle targeting to the yeast Golgi apparatus and ...
 79-242 4.83e-10

Dor1-like family; Dor1 is involved in vesicle targeting to the yeast Golgi apparatus and complexes with a number of other trafficking proteins, which include Sec34 and Sec35.


Pssm-ID: 427729  Cd Length: 315  Bit Score: 61.67  E-value: 4.83e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124486662   79 RECPLAQLMDSETDMVRQIRALDSDMQTLVYENYNKFISATDTIRKMKNDFRKMEDEMDRL---ATNMAV-ITNFSARiS 154
Cdd:pfam04124   5 STKPLEELEKEPELLAEEAAEILAQIQDLARKNYKTFIDAARATIAIRQLLGDIAKSLEQLvddIPKLTSsCVRFLED-A 83

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124486662  155 ATLQDRHERITKLAGVHAllrKLQFLFELPSRLTKCVELGAYGQAVRYQGRARAVLQQYQHLPSFRAIQDDCQVITARLA 234
Cdd:pfam04124  84 KEISETRRLNNLLLQNHD---QLLDLLELPQLMDTCIRTGYYSEALELAAYATRLRSRFPGSPVIQEIAAQVEEAMQTML 160


                  ....*...
gi 124486662  235 QQLRQRFR 242
Cdd:pfam04124 161 SQLIQQLR 168
 
Name Accession Description Interval E-value
Vps51 pfam08700
Vps51/Vps67; This family includes a presumed domain found in a number of components of ...
 63-147 4.94e-23

Vps51/Vps67; This family includes a presumed domain found in a number of components of vesicular transport. The VFT tethering complex (also known as GARP complex, Golgi associated retrograde protein complex, Vps53 tethering complex) is a conserved eukaryotic docking complex which is involved recycling of proteins from endosomes to the late Golgi. Vps51 (also known as Vps67) is a subunit of VFT and interacts with the SNARE Tlg1. Cog1_N is the N-terminus of the Cog1 subunit of the eight-unit Conserved Oligomeric Golgi (COG) complex that participates in retrograde vesicular transport and is required to maintain normal Golgi structure and function. The subunits are located in two lobes and Cog1 serves to bind the two lobes together probably via the highly conserved N-terminal domain of approximately 85 residues.


Pssm-ID: 462568  Cd Length: 86  Bit Score: 93.50  E-value: 4.94e-23
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124486662   63 LNGAHFDPEVYLDKLRRECPLAQLMDSETDMVRQIRALDSDMQTLVYENYNKFISATDTIRKMKNDFRKMEDEMDRLATN 142
Cdd:pfam08700   1 LDSPSFDADRYVSELLSKATLEELLQFESSLRSEIERLDSELKQLVYDNYRDLIKAADTISKMKSEMEQLSQKLSELKQA 80


                  ....*
gi 124486662  143 MAVIT 147
Cdd:pfam08700  81 LSKIA 85
Sec5 pfam15469
Exocyst complex component Sec5; This entry represents the Sec5 component of the exocyst ...
 62-264 2.16e-13

Exocyst complex component Sec5; This entry represents the Sec5 component of the exocyst complex which is an octomeric complex that mediates the tethering of post-Golgi secretory vesicles to the plasma membrane during exocytosis.


Pssm-ID: 464733  Cd Length: 810  Bit Score: 73.94  E-value: 2.16e-13
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124486662   62 DLNGAHFDPEVYLDKLRRECPLAQLMDSETDMVRQIRALDSDMQTLVYENYNKFISATDTIRKMKNDFRKMEDEMDRLAT 141
Cdd:pfam15469  29 LISSKSFNPKWFLSEIHNDTSFEDLKAGLDYLERSIESQSEELKSLVKSNFDKFVNCKDTLDAIYQQFKEDKTEAQLAKE 108

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124486662  142 NMAVITNFS-----ARISATLQ-----DRHERITKLAGVHALLRKLQFLFELPSRLTKCVELGAYGQAVRYQGRARAVLQ 211
Cdd:pfam15469 109 NESGTSKLEeslneAIRKANLVfkpvlERKDKADKLRNALGVLERNKFLFNLPKSLIESIAKGDYDVVINDYKKAKSLFE 188

                         170       180       190       200       210
                  ....*....|....*....|....*....|....*....|....*....|....*....
gi 124486662  212 Q-----YQHLPSFRAIQDDCQVITARLAQQLRQRFREGCSGAPE-QAECVELLLALGEP 264
Cdd:pfam15469 189 SrpksdITQVPVFKKVWAEVEKIIEEYRKQLWRKLLDPPLSVQEeQLKLISKLLELNVE 247
Dor1 pfam04124
Dor1-like family; Dor1 is involved in vesicle targeting to the yeast Golgi apparatus and ...
 79-242 4.83e-10

Dor1-like family; Dor1 is involved in vesicle targeting to the yeast Golgi apparatus and complexes with a number of other trafficking proteins, which include Sec34 and Sec35.


Pssm-ID: 427729  Cd Length: 315  Bit Score: 61.67  E-value: 4.83e-10
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124486662   79 RECPLAQLMDSETDMVRQIRALDSDMQTLVYENYNKFISATDTIRKMKNDFRKMEDEMDRL---ATNMAV-ITNFSARiS 154
Cdd:pfam04124   5 STKPLEELEKEPELLAEEAAEILAQIQDLARKNYKTFIDAARATIAIRQLLGDIAKSLEQLvddIPKLTSsCVRFLED-A 83

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124486662  155 ATLQDRHERITKLAGVHAllrKLQFLFELPSRLTKCVELGAYGQAVRYQGRARAVLQQYQHLPSFRAIQDDCQVITARLA 234
Cdd:pfam04124  84 KEISETRRLNNLLLQNHD---QLLDLLELPQLMDTCIRTGYYSEALELAAYATRLRSRFPGSPVIQEIAAQVEEAMQTML 160


                  ....*...
gi 124486662  235 QQLRQRFR 242
Cdd:pfam04124 161 SQLIQQLR 168
COG2 pfam06148
COG (conserved oligomeric Golgi) complex component, COG2; The COG complex comprises eight ...
 57-183 3.55e-04

COG (conserved oligomeric Golgi) complex component, COG2; The COG complex comprises eight proteins COG1-8. The COG complex plays critical roles in Golgi structure and function. The proposed function of the complex is to mediate the initial physical contact between transport vesicles and their membrane targets. A comparable role in tethering vesicles has been suggested for at least six additional large multisubunit complexes, including the exocyst, a complex that mediates trafficking to the plasma membrane. COG2 structure reveals a six-helix bundle with few conserved surface features but a general resemblance to recently determined crystal structures of four different exocyst subunits. These bundles inCOG2 may act as platforms for interaction with other trafficing proteins including SNAREs (soluble N-ethylmaleimide factor attachment protein receptors) and Rabs.


Pssm-ID: 399271  Cd Length: 133  Bit Score: 41.10  E-value: 3.55e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124486662   57 PLDPTDLNGAHFDPEVYLDKLRRECPLAQLMdsetdmvRQIRA----LDSDMQTLVYENYNKFISATDTIRKMKNDFRKM 132
Cdd:pfam06148   2 CFDKHEFLAPDFDPDAFVSELRHHVPLESLR-------RDLRAylklLRRELLELVNEDYADFVSLSTNLVGLDEKLNEV 74

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|.
gi 124486662  133 EDEmdrLATNMAVITNFSARISAtlqdRHERITKLAGVHALLRKLQFLFEL 183
Cdd:pfam06148  75 EVP---LLALRKEVESVRDELEA----RLEEVEQKLEERKALREKRESLKL 118
Vps54_N pfam10475
Vacuolar-sorting protein 54, of GARP complex; This is a family of vacuolar-sorting proteins 54, ...
 68-247 4.68e-04

Vacuolar-sorting protein 54, of GARP complex; This is a family of vacuolar-sorting proteins 54, from eukaryotes. Along with VPS52 and VPS53 this forms the Golgi-associated retrograde protein complex GARP. VPS54 is separated into N- and C-terminal regions each of which has a different function. This N-terminal family of is important for GARP complex assembly and stability, whereas the C-terminal domain, pfam07928, brings about localization to an early endocytic compartment.


Pssm-ID: 431303  Cd Length: 291  Bit Score: 43.11  E-value: 4.68e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124486662   68 FDPEVY-LDKLRRECPLAQLMDSETDMVR-QIRALDSDMQTLVYENYNKFISATDTIRKMKNDFRKMEDEMDRLATNMAV 145
Cdd:pfam10475  13 FDPILYeLKKLPDDGLDYESIEKQRTQLKrQHDVVSKKLSQLIMEQYNEFVQGMSQVREIEKDLQHSAVMCTNGRRYLDS 92

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 124486662  146 ITNFSARISATLQDRHERITKLAGVHALLRKLQFLFELPSRLTKCVELGAYGQAVRYQGRARAVLQQYQHlpsFRAIQD- 224
Cdd:pfam10475  93 AKKNLTRTSLGLLAKYRKRQLLKKLLMTLTTIKEIRDTDVRLQELLEDGNYPQAIQLLLECQQLATGYSH---YNCVQEl 169

                         170       180
                  ....*....|....*....|....*
gi 124486662  225 --DCQVITARLAQQLRQRFREGCSG 247
Cdd:pfam10475 170 ssKLQETLLLTEEKLDSVLLEVCQN 194
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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