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Conserved domains on  [gi|1812251883|ref|NP_001365533|]
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tubulin-specific chaperone cofactor E-like protein isoform c [Mus musculus]

Protein Classification

leucine-rich repeat domain-containing protein; ubiquitin family protein( domain architecture ID 13181512)

leucine-rich repeat (LRR) domain-containing protein may participate in protein-protein interactions| ubiquitin family protein belongs to a diverse class of protein modifier and gene expression regulatory proteins that participate in a number of cellular processes; has an N-terminal domain with similarity to the N-terminus of ubiquitin fusion degradation UFD1 which functions at a post-ubiquitation step in the ubiquitin fusion degradation (UFD) pathway

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Ubl_TBCEL cd17045
ubiquitin-like (Ubl) domain found in tubulin-specific chaperone cofactor E-like protein (TBCEL) ...
 212-297 1.52e-43

ubiquitin-like (Ubl) domain found in tubulin-specific chaperone cofactor E-like protein (TBCEL) and similar proteins; TBCEL, also termed leucine-rich repeat-containing protein 35 (LRRC35), or E-like (EL), is a novel regulator of tubulin stability, suggesting a link between tubulin turnover and vesicle transport. TBCEL is abundantly expressed in testis, but is also present in several tissues at a much lower level. It is required for the synchronous movement of the investment cones and is important for normal male fertility. TBCEL shows high sequence similarity to tubulin-specific chaperone cofactor E (TBCE), a component of the multimolecular complex required for tubulin heterodimer formation in all eukaryotic cells. It contains a leucine-rich repeat protein-protein interaction domain and a C-terminal ubiquitin-like (Ubl) domain, but does not harbor the cytoskeleton-associated protein with glycine-rich segment (CAP-Gly) domain found in TBCE.


:

Pssm-ID: 340565  Cd Length: 87  Bit Score: 143.93  E-value: 1.52e-43
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1812251883 212 DLRPQSSAKVEVHFNDQVEEMSIRLDQTVAELKKQLKTLVQLPTSSMLLYYFDHE-APFGPEEMKYSSRALHSFGIRDGD 290
Cdd:cd17045     1 DLTPPKSAKVTVHFEDQVESMDIDLDQTVAELKKQLKNLVGLPPSKMRLYYIDIEmSIFGPEELRFPSRALYSYNIRDGD 80


                  ....*..
gi 1812251883 291 KIFVESK 297
Cdd:cd17045    81 EILVEPK 87
LRR_9 super family cl25994
Leucine-rich repeat;
66-179 1.99e-09

Leucine-rich repeat;


The actual alignment was detected with superfamily member pfam14580:

Pssm-ID: 405295 [Multi-domain]  Cd Length: 175  Bit Score: 55.93  E-value: 1.99e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1812251883  66 EIRKLG--VMFPSLDTLVLANNHLNAIEEpadSLARLFPNLRSISLHKSGLQSWEDIDKLNSFPKLEEVRLLGIPLLqpy 143
Cdd:pfam14580  53 EIRKLDgfPLLRRLKTLLLNNNRICRIGE---GLGEALPNLTELILTNNNLQELGDLDPLASLKKLTFLSLLRNPVT--- 126

                          90       100       110
                  ....*....|....*....|....*....|....*.
gi 1812251883 144 TTEERRKLVVARLPSVSKLNGSVVTDGEREDSERFF 179
Cdd:pfam14580 127 NKPHYRLYVIYKVPQLRLLDFRKVKQKERQAAEKMF 162
 
Name Accession Description Interval E-value
Ubl_TBCEL cd17045
ubiquitin-like (Ubl) domain found in tubulin-specific chaperone cofactor E-like protein (TBCEL) ...
 212-297 1.52e-43

ubiquitin-like (Ubl) domain found in tubulin-specific chaperone cofactor E-like protein (TBCEL) and similar proteins; TBCEL, also termed leucine-rich repeat-containing protein 35 (LRRC35), or E-like (EL), is a novel regulator of tubulin stability, suggesting a link between tubulin turnover and vesicle transport. TBCEL is abundantly expressed in testis, but is also present in several tissues at a much lower level. It is required for the synchronous movement of the investment cones and is important for normal male fertility. TBCEL shows high sequence similarity to tubulin-specific chaperone cofactor E (TBCE), a component of the multimolecular complex required for tubulin heterodimer formation in all eukaryotic cells. It contains a leucine-rich repeat protein-protein interaction domain and a C-terminal ubiquitin-like (Ubl) domain, but does not harbor the cytoskeleton-associated protein with glycine-rich segment (CAP-Gly) domain found in TBCE.


Pssm-ID: 340565  Cd Length: 87  Bit Score: 143.93  E-value: 1.52e-43
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1812251883 212 DLRPQSSAKVEVHFNDQVEEMSIRLDQTVAELKKQLKTLVQLPTSSMLLYYFDHE-APFGPEEMKYSSRALHSFGIRDGD 290
Cdd:cd17045     1 DLTPPKSAKVTVHFEDQVESMDIDLDQTVAELKKQLKNLVGLPPSKMRLYYIDIEmSIFGPEELRFPSRALYSYNIRDGD 80


                  ....*..
gi 1812251883 291 KIFVESK 297
Cdd:cd17045    81 EILVEPK 87
LRR_9 pfam14580
Leucine-rich repeat;
66-179 1.99e-09

Leucine-rich repeat;


Pssm-ID: 405295 [Multi-domain]  Cd Length: 175  Bit Score: 55.93  E-value: 1.99e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1812251883  66 EIRKLG--VMFPSLDTLVLANNHLNAIEEpadSLARLFPNLRSISLHKSGLQSWEDIDKLNSFPKLEEVRLLGIPLLqpy 143
Cdd:pfam14580  53 EIRKLDgfPLLRRLKTLLLNNNRICRIGE---GLGEALPNLTELILTNNNLQELGDLDPLASLKKLTFLSLLRNPVT--- 126

                          90       100       110
                  ....*....|....*....|....*....|....*.
gi 1812251883 144 TTEERRKLVVARLPSVSKLNGSVVTDGEREDSERFF 179
Cdd:pfam14580 127 NKPHYRLYVIYKVPQLRLLDFRKVKQKERQAAEKMF 162
LRR COG4886
Leucine-rich repeat (LRR) protein [Transcription];
28-134 1.02e-06

Leucine-rich repeat (LRR) protein [Transcription];


Pssm-ID: 443914 [Multi-domain]  Cd Length: 414  Bit Score: 49.55  E-value: 1.02e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1812251883  28 LEELFLCLNDYETVSCPSVCCHSLKLLHITDNNLQDW-TEIRKLgvmfPSLDTLVLANNHLNAIEEPadsLARLfPNLRS 106
Cdd:COG4886   161 LKSLDLSNNQLTDLPEELGNLTNLKELDLSNNQITDLpEPLGNL----TNLEELDLSGNQLTDLPEP---LANL-TNLET 232

                          90       100
                  ....*....|....*....|....*...
gi 1812251883 107 ISLHKSGLqswEDIDKLNSFPKLEEVRL 134
Cdd:COG4886   233 LDLSNNQL---TDLPELGNLTNLEELDL 257
PPP1R42 cd21340
protein phosphatase 1 regulatory subunit 42; Protein phosphatase 1 regulatory subunit 42 ...
 48-173 1.12e-04

protein phosphatase 1 regulatory subunit 42; Protein phosphatase 1 regulatory subunit 42 (PPP1R42), also known as leucine-rich repeat-containing protein 67 (lrrc67) or testis leucine-rich repeat (TLRR) protein, plays a role in centrosome separation. PPP1R42 has been shown to interact with the well-conserved signaling protein phosphatase-1 (PP1) and thereby increasing PP1's activity, which counters centrosome separation. Inhibition of PPP1R42 expression increases the number of centrosomes per cell while its depletion reduces the activity of PP1 leading to activation of NEK2, the kinase responsible for phosphorylation of centrosomal linker proteins promoting centrosome separation.


Pssm-ID: 411060 [Multi-domain]  Cd Length: 220  Bit Score: 42.47  E-value: 1.12e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1812251883  48 CHSLKLLHITDNNLQ-------DWTEIRKLGvmfPSLDTLVLANNHLNAIEEpadsLARLfPNLRSISLHKSGLQSWEDI 120
Cdd:cd21340    89 LTNLEELHIENQRLPpgekltfDPRSLAALS---NSLRVLNISGNNIDSLEP----LAPL-RNLEQLDASNNQISDLEEL 160

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....
gi 1812251883 121 -DKLNSFPKLEEVRLLGIPLLQPYTTEERrklVVARLPSVSKLNGSVVTDGERE 173
Cdd:cd21340   161 lDLLSSWPSLRELDLTGNPVCKKPKYRDK---IILASKSLEVLDGKEITDTERQ 211
Ubiquitin_2 pfam14560
Ubiquitin-like domain; This entry contains ubiquitin-like domains.
 231-294 1.33e-03

Ubiquitin-like domain; This entry contains ubiquitin-like domains.


Pssm-ID: 405277  Cd Length: 83  Bit Score: 37.12  E-value: 1.33e-03
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1812251883 231 EMSIRLDQTVAELKKQLKTLVQLPTSSMLLYYFDHEAPFgPEEMKYSSRALHSFGIRDGDKIFV 294
Cdd:pfam14560  16 ERRFDKSLTIEELKEKLELITGTPPSSMRLQLYDDDDNL-VAKLDDDDALLGSYGVRDGMRIHV 78
 
Name Accession Description Interval E-value
Ubl_TBCEL cd17045
ubiquitin-like (Ubl) domain found in tubulin-specific chaperone cofactor E-like protein (TBCEL) ...
 212-297 1.52e-43

ubiquitin-like (Ubl) domain found in tubulin-specific chaperone cofactor E-like protein (TBCEL) and similar proteins; TBCEL, also termed leucine-rich repeat-containing protein 35 (LRRC35), or E-like (EL), is a novel regulator of tubulin stability, suggesting a link between tubulin turnover and vesicle transport. TBCEL is abundantly expressed in testis, but is also present in several tissues at a much lower level. It is required for the synchronous movement of the investment cones and is important for normal male fertility. TBCEL shows high sequence similarity to tubulin-specific chaperone cofactor E (TBCE), a component of the multimolecular complex required for tubulin heterodimer formation in all eukaryotic cells. It contains a leucine-rich repeat protein-protein interaction domain and a C-terminal ubiquitin-like (Ubl) domain, but does not harbor the cytoskeleton-associated protein with glycine-rich segment (CAP-Gly) domain found in TBCE.


Pssm-ID: 340565  Cd Length: 87  Bit Score: 143.93  E-value: 1.52e-43
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1812251883 212 DLRPQSSAKVEVHFNDQVEEMSIRLDQTVAELKKQLKTLVQLPTSSMLLYYFDHE-APFGPEEMKYSSRALHSFGIRDGD 290
Cdd:cd17045     1 DLTPPKSAKVTVHFEDQVESMDIDLDQTVAELKKQLKNLVGLPPSKMRLYYIDIEmSIFGPEELRFPSRALYSYNIRDGD 80


                  ....*..
gi 1812251883 291 KIFVESK 297
Cdd:cd17045    81 EILVEPK 87
LRR_9 pfam14580
Leucine-rich repeat;
66-179 1.99e-09

Leucine-rich repeat;


Pssm-ID: 405295 [Multi-domain]  Cd Length: 175  Bit Score: 55.93  E-value: 1.99e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1812251883  66 EIRKLG--VMFPSLDTLVLANNHLNAIEEpadSLARLFPNLRSISLHKSGLQSWEDIDKLNSFPKLEEVRLLGIPLLqpy 143
Cdd:pfam14580  53 EIRKLDgfPLLRRLKTLLLNNNRICRIGE---GLGEALPNLTELILTNNNLQELGDLDPLASLKKLTFLSLLRNPVT--- 126

                          90       100       110
                  ....*....|....*....|....*....|....*.
gi 1812251883 144 TTEERRKLVVARLPSVSKLNGSVVTDGEREDSERFF 179
Cdd:pfam14580 127 NKPHYRLYVIYKVPQLRLLDFRKVKQKERQAAEKMF 162
Ubl_TBCE cd17044
ubiquitin-like (Ubl) domain found in tubulin-folding cofactor E (TBCE) and similar proteins; ...
 239-295 8.10e-08

ubiquitin-like (Ubl) domain found in tubulin-folding cofactor E (TBCE) and similar proteins; TBCE, also termed tubulin-specific chaperone E, is a tubulin polymerizing protein involved in the second step of the tubulin folding pathway through cooperating in tubulin heterodimer dissociation both in vivo and in vitro. It may also be implicated in the maintenance of the neuronal microtubule network. Mutations in TBCE gene cause hypoparathyroidism, mental retardation and facial dysmorphism. TBCE contains an N-terminal cytoskeleton-associated protein with glycine-rich segment (CAP-Gly) domain, a leucine-rich repeat protein-protein interaction domain followed by leucine-rich repeat (LRR) domains, and a C-terminal ubiquitin-like (Ubl) domain. Ubiquitin is a protein modifier in eukaryotes that is involved in various cellular processes.


Pssm-ID: 340564  Cd Length: 83  Bit Score: 48.73  E-value: 8.10e-08
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*..
gi 1812251883 239 TVAELKKQLKTLVQLPTSSMLLYYFDHEAPFGPEEMKYSSRALHSFGIRDGDKIFVE 295
Cdd:cd17044    27 TVQKLKGLCERLFKLPASKQRLSYVSSEGPGIEIELDDDLRSLSFYSVEDGDTILVR 83
LRR COG4886
Leucine-rich repeat (LRR) protein [Transcription];
28-134 1.02e-06

Leucine-rich repeat (LRR) protein [Transcription];


Pssm-ID: 443914 [Multi-domain]  Cd Length: 414  Bit Score: 49.55  E-value: 1.02e-06
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1812251883  28 LEELFLCLNDYETVSCPSVCCHSLKLLHITDNNLQDW-TEIRKLgvmfPSLDTLVLANNHLNAIEEPadsLARLfPNLRS 106
Cdd:COG4886   161 LKSLDLSNNQLTDLPEELGNLTNLKELDLSNNQITDLpEPLGNL----TNLEELDLSGNQLTDLPEP---LANL-TNLET 232

                          90       100
                  ....*....|....*....|....*...
gi 1812251883 107 ISLHKSGLqswEDIDKLNSFPKLEEVRL 134
Cdd:COG4886   233 LDLSNNQL---TDLPELGNLTNLEELDL 257
Ubl_ubiquitin_like cd17039
ubiquitin-like (Ubl) domain found in ubiquitin and ubiquitin-like Ubl proteins; Ubiquitin-like ...
 221-294 6.75e-06

ubiquitin-like (Ubl) domain found in ubiquitin and ubiquitin-like Ubl proteins; Ubiquitin-like (Ubl) proteins have a similar ubiquitin (Ub) beta-grasp fold and attach to other proteins in a Ubl manner but with biochemically distinct roles. Ub and Ubl proteins conjugate and deconjugate via ligases and peptidases to covalently modify target polypeptides. Some Ubl domains have adaptor roles in Ub-signaling by mediating protein-protein interaction. Prokaryotic sulfur carrier proteins are Ub-related proteins that can be activated in an ATP-dependent manner. Polyubiquitination signals for a diverse set of cellular events via different isopeptide linkages formed between the C terminus of one ubiquitin (Ub) and the epsilon-amine of K6, K11, K27, K29, K33, K48, or K63 of a second Ub. One of these seven lysine residues (K27, Ub numbering) is conserved in this Ubl_ubiquitin_like family. K27-linked Ub chains are versatile and can be recognized by several downstream receptor proteins. K27 has roles beyond chain linkage, such as in Ubl NEDD8 (which contains many of the same lysines (K6, K11, K27, K33, K48) as Ub) where K27 has a role (other than conjugation) in the mechanism of protein neddylation.


Pssm-ID: 340559 [Multi-domain]  Cd Length: 68  Bit Score: 42.97  E-value: 6.75e-06
                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 1812251883 221 VEVHFND-QVEEMSIRLDQTVAELKKQLKTLVQLPTSSMLLYYFDheapfgpEEMKySSRALHSFGIRDGDKIFV 294
Cdd:cd17039     1 ITVKTLDgKTYTVEVDPDDTVADLKEKIEEKTGIPVEQQRLIYNG-------KELK-DDKTLSDYGIKDGSTIHL 67
PPP1R42 cd21340
protein phosphatase 1 regulatory subunit 42; Protein phosphatase 1 regulatory subunit 42 ...
 48-173 1.12e-04

protein phosphatase 1 regulatory subunit 42; Protein phosphatase 1 regulatory subunit 42 (PPP1R42), also known as leucine-rich repeat-containing protein 67 (lrrc67) or testis leucine-rich repeat (TLRR) protein, plays a role in centrosome separation. PPP1R42 has been shown to interact with the well-conserved signaling protein phosphatase-1 (PP1) and thereby increasing PP1's activity, which counters centrosome separation. Inhibition of PPP1R42 expression increases the number of centrosomes per cell while its depletion reduces the activity of PP1 leading to activation of NEK2, the kinase responsible for phosphorylation of centrosomal linker proteins promoting centrosome separation.


Pssm-ID: 411060 [Multi-domain]  Cd Length: 220  Bit Score: 42.47  E-value: 1.12e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1812251883  48 CHSLKLLHITDNNLQ-------DWTEIRKLGvmfPSLDTLVLANNHLNAIEEpadsLARLfPNLRSISLHKSGLQSWEDI 120
Cdd:cd21340    89 LTNLEELHIENQRLPpgekltfDPRSLAALS---NSLRVLNISGNNIDSLEP----LAPL-RNLEQLDASNNQISDLEEL 160

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....
gi 1812251883 121 -DKLNSFPKLEEVRLLGIPLLQPYTTEERrklVVARLPSVSKLNGSVVTDGERE 173
Cdd:cd21340   161 lDLLSSWPSLRELDLTGNPVCKKPKYRDK---IILASKSLEVLDGKEITDTERQ 211
Ubl_TBCB cd01789
ubiquitin-like (Ubl) domain found in tubulin-folding cofactor B (TBCB) and similar proteins; ...
 236-294 5.45e-04

ubiquitin-like (Ubl) domain found in tubulin-folding cofactor B (TBCB) and similar proteins; TBCB, also termed cytoskeleton-associated protein 1, or cytoskeleton-associated protein CKAPI, or tubulin-specific chaperone B, is one of protein cofactors A through E that is required for the folding of tubulins prior to their incorporation into microtubules and heterodimer assembly. TBCB comprises an N-terminal ubiquitin-like (Ubl) domain and a C-terminal cytoskeleton-associated protein with glycine-rich segment (CAP-Gly) domain. The Ubl domain of TBCB is essential for proper folding and assembly of tubulin alpha. It has a beta-grasp Ubl fold, a common structure involved in protein-protein interactions. Ubiquitin (Ub) is a protein modifier in eukaryotes that is involved in various cellular processes, including transcriptional regulation, cell cycle control, and DNA repair. TBC-A through E are necessary for the biogenesis of microtubules and for cell viability.


Pssm-ID: 340487  Cd Length: 80  Bit Score: 37.93  E-value: 5.45e-04
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|....*....
gi 1812251883 236 LDQTVAELKKQLKTLVQLPTSSMLLYYFDHEAPFgPEEMKYSSRALHSFGIRDGDKIFV 294
Cdd:cd01789    21 LSLTIGELKEKLELITGTPPSSMKLQLYDEDGKL-IGTLDDDDALLGSYPVRDGMRIHV 78
LRR COG4886
Leucine-rich repeat (LRR) protein [Transcription];
27-151 1.06e-03

Leucine-rich repeat (LRR) protein [Transcription];


Pssm-ID: 443914 [Multi-domain]  Cd Length: 414  Bit Score: 40.30  E-value: 1.06e-03
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1812251883  27 RLEELFLCLNDYETVSCPSVCCHSLKLLHITDNNLQDWTEIRKLgvmfPSLDTLVLANNHLNAIEEPADslarlFPNLRS 106
Cdd:COG4886   206 NLEELDLSGNQLTDLPEPLANLTNLETLDLSNNQLTDLPELGNL----TNLEELDLSNNQLTDLPPLAN-----LTNLKT 276

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*
gi 1812251883 107 ISLHKSGLQSWedidKLNSFPKLEEVRLLGIPLLQPYTTEERRKL 151
Cdd:COG4886   277 LDLSNNQLTDL----KLKELELLLGLNSLLLLLLLLNLLELLILL 317
Ubiquitin_2 pfam14560
Ubiquitin-like domain; This entry contains ubiquitin-like domains.
 231-294 1.33e-03

Ubiquitin-like domain; This entry contains ubiquitin-like domains.


Pssm-ID: 405277  Cd Length: 83  Bit Score: 37.12  E-value: 1.33e-03
                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1812251883 231 EMSIRLDQTVAELKKQLKTLVQLPTSSMLLYYFDHEAPFgPEEMKYSSRALHSFGIRDGDKIFV 294
Cdd:pfam14560  16 ERRFDKSLTIEELKEKLELITGTPPSSMRLQLYDDDDNL-VAKLDDDDALLGSYGVRDGMRIHV 78
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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