calponin homology (CH) domain found in transgelin; Transgelin, also called 22 kDa actin-binding protein, protein WS3-10, or smooth muscle protein 22-alpha (SM22-alpha), acts as an actin cross-linking/gelling protein that may be involved in calcium interactions and in regulating contractile properties of the cell. It may also contribute to replicative senescence. Transgelin contains a single copy of the CH domain. CH domains are actin filament (F-actin) binding motifs.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6755714   23 DEELEERLVEWIVVQCGPDVGRPDRGRLGFQVWLKNGVILSKLVNSLYPEGSKPVKVPENPPSMVFKQMEQVAQFLKAAE 102
Cdd:cd21279   1 DEELEERLVEWIVVQCGPDVGRPDRGRLGFQVWLKNGVVLSKLVNSLYPDGSKPVKIPDNPPSMVFKQMEQVAQFLKAAE 80

                        90       100       110       120
                ....*....|....*....|....*....|....*....|.
gi 6755714  103 DYGVIKTDMFQTVDLYEGKDMAAVQRTLMALGSLAVTKNDG 143
Cdd:cd21279  81 DYGVVKTDMFQTVDLYEGKDMAAVQRTLVALGSLAVTKNDG 121

calponin homology (CH) domain found in transgelin; Transgelin, also called 22 kDa actin-binding protein, protein WS3-10, or smooth muscle protein 22-alpha (SM22-alpha), acts as an actin cross-linking/gelling protein that may be involved in calcium interactions and in regulating contractile properties of the cell. It may also contribute to replicative senescence. Transgelin contains a single copy of the CH domain. CH domains are actin filament (F-actin) binding motifs.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6755714   23 DEELEERLVEWIVVQCGPDVGRPDRGRLGFQVWLKNGVILSKLVNSLYPEGSKPVKVPENPPSMVFKQMEQVAQFLKAAE 102
Cdd:cd21279   1 DEELEERLVEWIVVQCGPDVGRPDRGRLGFQVWLKNGVVLSKLVNSLYPDGSKPVKIPDNPPSMVFKQMEQVAQFLKAAE 80

                        90       100       110       120
                ....*....|....*....|....*....|....*....|.
gi 6755714  103 DYGVIKTDMFQTVDLYEGKDMAAVQRTLMALGSLAVTKNDG 143
Cdd:cd21279  81 DYGVVKTDMFQTVDLYEGKDMAAVQRTLVALGSLAVTKNDG 121

Calponin homology (CH) domain; The CH domain is found in both cytoskeletal proteins and signal transduction proteins. The CH domain is involved in actin binding in some members of the family. However in calponins there is evidence that the CH domain is not involved in its actin binding activity. Most member proteins have from two to four copies of the CH domain, however some proteins such as calponin have only a single copy.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6755714     24 EELEERLVEWIVVQCGPDVGRPDRGrlGFQVWLKNGVILSKLVNSLYPEGSKPVKVPENPpsmvFKQMEQVAQFLKAAE- 102
Cdd:pfam00307   1 LELEKELLRWINSHLAEYGPGVRVT--NFTTDLRDGLALCALLNKLAPGLVDKKKLNKSE----FDKLENINLALDVAEk 74

                          90       100       110
                  ....*....|....*....|....*....|....*..
gi 6755714    103 DYGVIKTDMfQTVDLYEGKDMaAVQRTLMALGSLAVT 139
Cdd:pfam00307  75 KLGVPKVLI-EPEDLVEGDNK-SVLTYLASLFRRFQA 109

Calponin family repeat;

                          10        20
                  ....*....|....*....|....*
gi 6755714    175 IGLQMGSNRGASQAGMTGYGRPRQI 199
Cdd:pfam00402   1 IGLQMGTNKGASQSGMTAFGAVRHI 25

calponin homology (CH) domain found in transgelin; Transgelin, also called 22 kDa actin-binding protein, protein WS3-10, or smooth muscle protein 22-alpha (SM22-alpha), acts as an actin cross-linking/gelling protein that may be involved in calcium interactions and in regulating contractile properties of the cell. It may also contribute to replicative senescence. Transgelin contains a single copy of the CH domain. CH domains are actin filament (F-actin) binding motifs.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6755714   23 DEELEERLVEWIVVQCGPDVGRPDRGRLGFQVWLKNGVILSKLVNSLYPEGSKPVKVPENPPSMVFKQMEQVAQFLKAAE 102
Cdd:cd21279   1 DEELEERLVEWIVVQCGPDVGRPDRGRLGFQVWLKNGVVLSKLVNSLYPDGSKPVKIPDNPPSMVFKQMEQVAQFLKAAE 80

                        90       100       110       120
                ....*....|....*....|....*....|....*....|.
gi 6755714  103 DYGVIKTDMFQTVDLYEGKDMAAVQRTLMALGSLAVTKNDG 143
Cdd:cd21279  81 DYGVVKTDMFQTVDLYEGKDMAAVQRTLVALGSLAVTKNDG 121

calponin homology (CH) domain found in transgelin-2; Transgelin-2, also called epididymis tissue protein Li 7e, or SM22-alpha homolog, acts as an actin-binding protein that induces actin gelation and regulates the actin cytoskeleton. It may participate in the development and progression of multiple cancers. It contains a single copy of the CH domain. CH domains are actin filament (F-actin) binding motifs.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6755714   18 IEKKYDEELEERLVEWIVVQCGPDVGRPDRGRLGFQVWLKNGVILSKLVNSLYPEGSKPV-KVPENppSMVFKQMEQVAQ 96
Cdd:cd21280   1 IDKQYDADLEQILIQWITAQCGKQVGRPQPGRENFQNWLKDGTVLCHLINSLYPKGQAPVkKIQAS--TMAFKQMEQISQ 78

                        90       100       110       120       130
                ....*....|....*....|....*....|....*....|....*....|....*....
gi 6755714   97 FLKAAEDYGVIKTDMFQTVDLYEGKDMAAVQRTLMALGSLAVTKNDGNYRGDPNWFMKK 155
Cdd:cd21280  79 FLQAAERYGINTTDIFQTVDLWEGKNMASVQRTLMNLGGLAVTRDDGLFVGDPNWFPKK 137

calponin homology (CH) domain found in Drosophila melanogaster muscle-specific protein 20 (dMP20) and similar domains; This subfamily contains Drosophila melanogaster muscle-specific protein 20 (dMP20), Echinococcus granulosus myophilin, Dictyostelium discoideum Rac guanine nucleotide exchange factor B (also called Trix), and similar proteins. dMP20 is present only in the synchronous muscles of D. melanogaster. It may be involved in the system linking the nerve impulse with the contraction or the relaxation process. Trix is involved in the regulation of the late steps of the endocytic pathway. dMP20 contains a single copy of the CH domain, while Trix (triple CH-domain array exchange factor) contains three, two type 3 CH domains which are included in this model, and one type 1 CH domain that is not included in this subfamily, but is part of the superfamily. CH domains are actin filament (F-actin) binding motifs.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6755714   21 KYDEELEERLVEWI--VVQCGPDVGRPdrgrlgFQVWLKNGVILSKLVNSLYPeGSKPvKVpeNPPSMVFKQMEQVAQFL 98
Cdd:cd21207   1 KRDPALEAEALDWIeaVTGEKLDDGKD------YEDVLKDGVILCKLINILKP-GSVK-KI--NTSKMAFKLMENIENFL 70

                        90       100       110
                ....*....|....*....|....*....|....*..
gi 6755714   99 KAAEDYGVIKTDMFQTVDLYEGKDMAAVQRTLMALGS 135
Cdd:cd21207  71 TACKGYGVPKTDLFQTVDLYEKKNIPQVTNCLFALGR 107

calponin homology (CH) domain superfamily; CH domains are actin filament (F-actin) binding motifs, which may be present as a single copy or in tandem repeats (which increase binding affinity). They either function as autonomous actin binding motifs or serve a regulatory function. CH domains are found in cytoskeletal and signal transduction proteins, including actin-binding proteins like spectrin, alpha-actinin, dystrophin, utrophin, and fimbrin, as well as proteins essential for regulation of cell shape (cortexillins), and signaling proteins (Vav).

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6755714   27 EERLVEWIVVQCGPDVGRPDRGrlgFQVWLKNGVILSKLVNSLYPeGSKPVKVpeNPPSMVFKQMEQVAQFLKAAEDYGV 106
Cdd:cd00014   1 EEELLKWINEVLGEELPVSITD---LFESLRDGVLLCKLINKLSP-GSIPKIN--KKPKSPFKKRENINLFLNACKKLGL 74

                        90       100
                ....*....|....*....|....*....
gi 6755714  107 IKTDMFQTVDLYEGKDMAAVQRTLMALGS 135
Cdd:cd00014  75 PELDLFEPEDLYEKGNLKKVLGTLWALAL 103

Calponin [Cytoskeleton];

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6755714   14 VQSKIEKKYDEELEERLVEWIVVQCGPDVGRPdrgrLGFQVWLKNGVILSKLVNSlypegSKPVKVPENPPSMVFKQMEQ 93
Cdd:COG5199   2 VNTKNRCPGMDKQQKEVTLWIETVLGEKFEPP----GDLLSLLKDGVRLCRILNE-----ASPLDIKYKESKMPFVQMEN 72

                        90       100       110       120       130       140       150       160
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6755714   94 VAQFLKAAEDYGVIKTDMFQTVDLYEGKDMAAVQRTLMALGSLAVTKNDGNYRG-DPNWFMKKaqehKRDFTDSQ-LQEG 171
Cdd:COG5199  73 ISSFINGLKKLRVPEYELFQTNDLFEAKDLRQVVICLYSLSRYAQKERMFSGPFlGPHLATKK----PRVFSSQEvLDRS 148

                       170
                ....*....|....*...
gi 6755714  172 KHVIGLQMGSNRGASQAG 189
Cdd:COG5199 149 KGAIHLQYGYSDLSEQST 166

Calponin homology (CH) domain; The CH domain is found in both cytoskeletal proteins and signal transduction proteins. The CH domain is involved in actin binding in some members of the family. However in calponins there is evidence that the CH domain is not involved in its actin binding activity. Most member proteins have from two to four copies of the CH domain, however some proteins such as calponin have only a single copy.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6755714     24 EELEERLVEWIVVQCGPDVGRPDRGrlGFQVWLKNGVILSKLVNSLYPEGSKPVKVPENPpsmvFKQMEQVAQFLKAAE- 102
Cdd:pfam00307   1 LELEKELLRWINSHLAEYGPGVRVT--NFTTDLRDGLALCALLNKLAPGLVDKKKLNKSE----FDKLENINLALDVAEk 74

                          90       100       110
                  ....*....|....*....|....*....|....*..
gi 6755714    103 DYGVIKTDMfQTVDLYEGKDMaAVQRTLMALGSLAVT 139
Cdd:pfam00307  75 KLGVPKVLI-EPEDLVEGDNK-SVLTYLASLFRRFQA 109

calponin homology (CH) domain found in calponin-2; Calponin-2 (CNN2), also called neutral calponin, or smooth muscle calponin H2, is an actin cytoskeleton-associated regulatory protein that inhibits the activity of myosin-ATPase and cytoskeleton dynamics. It contains a single copy of the CH domain. CH domains are actin filament (F-actin) binding motifs.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6755714   23 DEELEERLVEWIVVQCGPDVGrPDrgrlgFQVWLKNGVILSKLVNSLYPEGSKPVkvpeNPPSMVFKQMEQVAQFLKAAE 102
Cdd:cd21283   1 DPQKEAELRTWIEGLTGRSIG-PD-----FQKGLKDGVILCELMNKLQPGSVPKI----NRSMQNWHQLENLSNFIKAMV 70

                        90       100       110
                ....*....|....*....|....*....|....*...
gi 6755714  103 DYGVIKTDMFQTVDLYEGKDMAAVQRTLMALGSLAVTK 140
Cdd:cd21283  71 SYGMKPVDLFEANDLFESGNMTQVQVSLLALAGMAKTK 108

calponin homology (CH) domain found in the IQ motif containing GTPase activating protein family; Members of the IQ motif containing GTPase activating protein (IQGAP) family are associated with the Ras GTP-binding protein and act as essential regulators of cytoskeletal function. There are three known IQGAP family members: IQGAP1, IQGAP2, and IQGAP3. They are multi-domain molecules having a calponin-homology (CH) domain which binds F-actin, IQGAP-specific repeats, a single WW domain, four IQ motifs that mediate interactions with calmodulin, and a RasGAP related domain that binds active Rho family GTPases. IQGAP1 negatively regulates Ras family GTPases by stimulating their intrinsic GTPase activity. It lacks GAP activity. Both IQGAP1 and IQGAP2 specifically bind to Cdc42 and Rac1, but not to RhoA. Despite similarities to part of the sequence of RasGAP, neither IQGAP1 nor IQGAP2 interacts with Ras. IQGAP3 regulates the organization of the cytoskeleton under the regulation of Rac1 and Cdc42 in neuronal cells. The depletion of IQGAP3 is shown to impair neurite or axon outgrowth in neuronal cells with disorganized cytoskeleton.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6755714   56 LKNGVILSKLVNSLYPEGSKPVKVPENPPSmvFKQMEQVAQFLKAAEDYGVIKTDMFQTVDLYEGKDMAAVQRTLMALGS 135
Cdd:cd21206  35 LRNGVVLAKLANKFAPKLVPLKKIYDVGLQ--FRHTDNINHFLRALKKIGLPKIFHFETTDLYEKKNIPKVIYCLHALSL 112

                .
gi 6755714  136 L 136
Cdd:cd21206 113 L 113

calponin homology (CH) domain found in the growth arrest-specific protein 2 family; The growth arrest-specific protein 2 (GAS-2) family includes GAS-2, and GAS-2 like proteins, GAS2L1-3. GAS-2 may play a role in apoptosis by acting as a cell death substrate for caspases. GAS2L1 (also called GAS2-related protein on chromosome 22 or growth arrest-specific protein 2-like 1) and GAS2L2 (also called GAS2-related protein on chromosome 17 or growth arrest-specific protein 2-like 2) may be involved in the cross-linking of microtubules and microfilaments. GAS2L3, also called GAS2-like protein 3, is a cytoskeletal linker protein that may promote and stabilize the formation of the actin and microtubule network. Members of this family contain a single copy of the CH domain at the N-terminal region. CH domains are actin filament (F-actin) binding motifs.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6755714   20 KKYDEELEERLVEWIVVQCGPDVGrPDrgrlGFQVWLKNGVILSKLVNSL--------------YPEGSKPVKVPENPPS 85
Cdd:cd21204   1 EEALLPMKEDLAEWLNDLLGDDLT-PD----NFLDELRNGVVLCQLAQKIqeaaekareagkknGPPPSYKLKCNENAKP 75

                        90       100       110       120       130
                ....*....|....*....|....*....|....*....|....*....|....
gi 6755714   86 MVFKQMEQVAQFLKAAEDYGVIKTDMFQTVDLYEGKDMAAVQRTLMALGSLAVT 139
Cdd:cd21204  76 GSFFARDNVANFLRWCRKLGVDEVLLFESEDLVLHKNPRQVLLCLLELARIAAR 129

calponin homology (CH) domain found in VAV proteins; VAV proteins function both as cytoplasmic guanine nucleotide exchange factors (GEFs) for Rho GTPases and as scaffold proteins, and they play important roles in cell signaling by coupling cell surface receptors to various effector functions. They play key roles in processes that require cytoskeletal reorganization including immune synapse formation, phagocytosis, cell spreading, and platelet aggregation, among others. Vertebrates have three VAV proteins (VAV1, VAV2, and VAV3). VAV proteins contain several domains that enable their function: N-terminal calponin homology (CH), acidic, RhoGEF (also called Dbl-homologous or DH), Pleckstrin Homology (PH), C1 (zinc finger), SH2, and two SH3 domains. This model corresponds to the CH domain, an actin-binding domain which is present as a single copy in VAV proteins.

                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 6755714   56 LKNGVILSKLVNSLYPEGSKPVKVPENPPSMVFKQMEQVAQFLKAAED-YGVIKTDMFQTVDLYEGKDMAAVQRTL 130
Cdd:cd21201  36 LRDGVLLCQLLNRLSPGSVDDREINLRPQMSQFLCLKNIRTFLQACRTvFGLRSADLFEPEDLYDVTNFGKVIRTL 111

calponin homology (CH) domain found in LIM domain only protein 7 and similar proteins; This family includes LIM domain only protein 7 (LMO-7) and LIM and calponin homology domains-containing protein 1 (LIMCH1), and similar proteins. LMO-7, also called F-box only protein 20, or LOMP, is a transcription regulator for expression of many Emery-Dreifuss muscular dystrophy (EDMD)-relevant genes. It binds to alpha-actinin and AF6/afadin at adherens junctions for epithelial cell-cell adhesion. LIMCH1 acts as an actin stress fiber-associated protein that activates the non-muscle myosin IIa complex by promoting the phosphorylation of its regulatory subunit MRLC/MYL9. It positively regulates actin stress fiber assembly and stabilizes focal adhesions, and therefore negatively regulates cell spreading and cell migration. Members of this family contain a single copy of the CH domain at the N-terminus. CH domains are actin filament (F-actin) binding motifs.

                        10        20        30        40        50        60
                ....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 6755714   52 FQVWLKNGVILSKLVNSLYPEGSKPVKVPENPpsmvFKQMEQVAQFLKAAEDYGVIKTDMFQTVDLYE 119
Cdd:cd21208  22 FRESLEDGILLCELINAIKPGSIKKINRLPTP----IAGLDNLNLFLKACEDLGLKDSQLFDPTDLQD 85

calponin homology (CH) domain found in VAV3 protein and similar proteins; VAV3 is ubiquitously expressed and functions as a phosphorylation-dependent guanine nucleotide exchange factor (GEF) for RhoA, RhoG, and Rac1. Its function has been implicated in the hematopoietic, bone, cerebellar, and cardiovascular systems. VAV3 is essential in axon guidance in neurons that control blood pressure and respiration. It is overexpressed in prostate cancer cells and it plays a role in regulating androgen receptor transcriptional activity. VAV proteins contain several domains that enable their function: N-terminal calponin homology (CH), acidic, RhoGEF (also called Dbl-homologous or DH), Pleckstrin Homology (PH), C1 (zinc finger), SH2, and two SH3 domains. The model corresponds to CH domain, an actin-binding domain which is present as a single copy in VAV3 protein.

                        10        20        30        40        50        60        70
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 6755714   56 LKNGVILSKLVNSLYPEGSKPVKVPENPPSMVFKQMEQVAQFLKAA-EDYGVIKTDMFQTVDLYEGKDMAAVQRTLMAL 133
Cdd:cd21264  36 LRDGVLLCQLLNNLRPHSINLKEINLRPQMSQFLCLKNIRTFLSACcETFGMRKSELFEAFDLFDVRDFGKVIETLSKL 114

calponin homology (CH) domain found in the leucine-rich repeat and calponin homology domain-containing protein family; The leucine-rich repeat and calponin homology domain-containing protein (LRCH) family includes LRCH1-4. LRCH1, also called calponin homology domain-containing protein 1, or neuronal protein 81 (NP81), acts as a negative regulator of GTPase Cdc42 by sequestering Cdc42-guanine exchange factor DOCK8. LRCH2 may play a role in the organization of the cytoskeleton. LRCH3 is part of the DISP complex and may regulate the association of septins with actin and thereby regulate the actin cytoskeleton. LRCH4, also called leucine-rich repeat neuronal protein 4, or leucine-rich neuronal protein, acts as a novel Toll-like receptor (TLR) accessory protein that regulates the innate immune response. Members of this family contain a single copy of the CH domain at the C-terminus. CH domains are actin filament (F-actin) binding motifs.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6755714   56 LKNGVILSKLVNSLYPEGSKPVKVPEN--PPSMVFKQMEQVAQFLKAAEDYGVIKTDMFQTVDLYEGKDMAAVQRTLMAL 133
Cdd:cd21205  26 LMDGVVLCHLANHVRPRSVPSIHVPSPavPKLSMAKCRRNVENFLEACRKLGVPEERLCSPGDILEEKGLVRVAVTVQAL 105

                ..
gi 6755714  134 GS 135
Cdd:cd21205 106 LQ 107

calponin homology (CH) domain found in leucine-rich repeat and calponin homology domain-containing protein 4; Leucine-rich repeat and calponin homology domain-containing protein 4 (LRCH4), also called leucine-rich repeat neuronal protein 4, or leucine-rich neuronal protein, acts as a novel Toll-like receptor (TLR) accessory protein that regulates the innate immune response. LRCH4 contains a single copy of the CH domain at the C-terminus. CH domains are actin filament (F-actin) binding motifs.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6755714   56 LKNGVILSKLVNSLYPEGSKPVKVPEN--PPSMVFKQMEQVAQFLKAAEDYGVIKTDMFQTVDLYEGKDmAAVQRTLMAL 133
Cdd:cd21273  29 LSNGAVLCQLANQLRPRSVSIIHVPSPavPKLSKAKCRKNVENFIEACRKMGVPEVDLCSPSDVLLQGP-AAVLRTVLAL 107

calponin homology (CH) domain found in leucine-rich repeat and calponin homology domain-containing protein 3; Leucine-rich repeat and calponin homology domain-containing protein 3 (LRCH3) is part of the DISP (DOCK7-Induced Septin disPlacement) complex. It may regulate the association of septins with actin and thereby regulate the actin cytoskeleton. LRCH3 contains a single copy of the CH domain at the C-terminus. CH domains are actin filament (F-actin) binding motifs.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 6755714   56 LKNGVILSKLVNSLYPEGSKPVKVPEN--PPSMVFKQMEQVAQFLKAAEDYGVIKTDMFQTVDLYEGKDMAAVQRTLMAL 133
Cdd:cd21272  26 LTDGVVLCHLANHVRPRSVPSIHVPSPavPKLTMAKCRRNVENFLEACRRIGVPQEQLCLPLHILEEKGLSQVAVTVQAL 105

                ....
gi 6755714  134 GSLA 137
Cdd:cd21272 106 LELA 109