|
Name |
Accession |
Description |
Interval |
E-value |
| PolY_Pol_iota |
cd01703 |
DNA Polymerase iota; Pol iota, also called Rad30B, is a translesion synthesis (TLS) polymerase. ... |
51-431 |
1.18e-174 |
|
DNA Polymerase iota; Pol iota, also called Rad30B, is a translesion synthesis (TLS) polymerase. Translesion synthesis is a process that allows the bypass of a variety of DNA lesions. TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. Pol iota is thought to be one of the least efficient polymerases, particularly when opposite pyrimidines; it can incorporate the correct nucleotide opposite a purine much more efficiently than opposite a pyrimidine, and prefers to insert guanosine instead of adenosine opposite thymidine. Pol iota is believed to use Hoogsteen rather than Watson-Crick base pairing, which may explain the varying efficiency for different template nucleotides.
Pssm-ID: 176457 [Multi-domain] Cd Length: 379 Bit Score: 505.08 E-value: 1.18e-174
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226958460 51 VHVDLDCFYAQVEMISNPELKDRPLGVQQKYLVVTCNYEARKLGVRKLMNVRDAKEKCPQLVLVNGEDLSRYREMSYKVT 130
Cdd:cd01703 1 IHLDLDCFYAQVEEIRDPSLKSKPLGIQQKYIVVTCNYEARRLGVKKLMSIKDAKEICPDLVLVNGEDLTPFRDMSKKVY 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226958460 131 ELLEEFSPA--VERLGFDENFVDLTEMvekrlqqlpseevpsvtvfghvynnqsvnlhnimhrRLVVGSQIAAEMREAMY 208
Cdd:cd01703 81 RLLRSYSWNdrVERLGFDENFMDVTEM------------------------------------RLLVASHIAYEMRERIE 124
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226958460 209 NQLGLTGCAGVAPNKLLAKLVSGVFKPNQQTVLLPESCQHLI--HSLNHIKEIPGIGYKTAKRLEVLGINSVHDLQTFPI 286
Cdd:cd01703 125 NELGLTCCAGIASNKLLAKLVGSVNKPNQQTTLLPPSCADLMdfMDLHDLRKIPGIGYKTAAKLEAHGISSVRDLQEFSN 204
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226958460 287 ---------------KTLEKELGIAIAQRIQQLSFGEDKSPV-TPSGPPQSFSEEDTFKKCSSEV--EAKAKIEELLSSL 348
Cdd:cd01703 205 rnrqtvgaapsllelLLMVKEFGEGIGQRIWKLLFGRDTSPVkPASDFPQQISIEDSYKKCSLEEirEARNKIEELLASL 284
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226958460 349 LTRVCQ--------DGRKPHTVRLVIRRYSD--KHCNRESRQCPIPSHVIQKLGTGNHDSMPPLIDILMKLFRNMVNVK- 417
Cdd:cd01703 285 LERMKQdlqevkagDGRRPHTLRLTLRRYTStkKHYNRESKQAPIPSHVFQKLTGGNEIAARPIEKILMRLFRELVPPKn 364
|
410
....*....|....*
gi 226958460 418 -MPFHLTLMSVCFCN 431
Cdd:cd01703 365 vKGFNLTLLNVCFTN 379
|
|
| DinP |
COG0389 |
Nucleotidyltransferase/DNA polymerase DinP involved in DNA repair [Replication, recombination ... |
48-422 |
2.23e-82 |
|
Nucleotidyltransferase/DNA polymerase DinP involved in DNA repair [Replication, recombination and repair];
Pssm-ID: 440158 [Multi-domain] Cd Length: 336 Bit Score: 265.08 E-value: 2.23e-82
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226958460 48 RVIVHVDLDCFYAQVEMISNPELKDRPLGV---QQKYLVVTCNYEARKLGVRKLMNVRDAKEKCPQLVLVNGeDLSRYRE 124
Cdd:COG0389 1 RRILHVDMDAFYASVEQRDRPELRGKPVAVggdNNRGVVAAASYEARAFGVRSGMPLFQARRLCPDLVVLPP-DFELYRD 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226958460 125 MSYKVTELLEEFSPAVERLGFDENFVDLTEMveKRLqqLPSEEvpsvtvfghvynnqsvnlhnimhrrlvvgsQIAAEMR 204
Cdd:COG0389 80 VSRRVMAILERYTPLVEPLSIDEAFLDVTGS--ARL--FGSAE------------------------------AIARRIR 125
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226958460 205 EAMYNQLGLTGCAGVAPNKLLAKLVSGVFKPNQQTVLLPESCQHLIHSLnHIKEIPGIGYKTAKRLEVLGINSVHDLQTF 284
Cdd:COG0389 126 RRIRRETGLTVSVGIAPNKFLAKIASDLAKPDGLTVIPPGEVAAFLAPL-PVEKLWGVGPKTAEKLARLGIRTIGDLAAL 204
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226958460 285 PIKTLEKELGIAiAQRIQQLSFGEDKSPVTPSGPPQSFSEEDTFKKcssEVEAKAKIEELLSSLLTRVC----QDGRKPH 360
Cdd:COG0389 205 PRAELRRRFGKV-GERLYRLARGIDPRPVEPRRPRKSIGVERTFGE---DLTDLEELEAALRRLAERLAerlrRQGLGAR 280
|
330 340 350 360 370 380
....*....|....*....|....*....|....*....|....*....|....*....|..
gi 226958460 361 TVRLVIrRYSDKHCNRESRQCPIPShviqklgtgnhDSMPPLIDILMKLFRNMVNVKMPFHL 422
Cdd:COG0389 281 TVTVKL-RTSDFRTTTRSRTLPEPT-----------DDTAELLRAARELLERIYRPGRPVRL 330
|
|
| PRK02406 |
PRK02406 |
DNA polymerase IV; Validated |
56-371 |
9.48e-64 |
|
DNA polymerase IV; Validated
Pssm-ID: 235035 [Multi-domain] Cd Length: 343 Bit Score: 216.14 E-value: 9.48e-64
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226958460 56 DCFYAQVEMISNPELKDRPLGV----QQKYLVVTCNYEARKLGVRKLMNVRDAKEKCPQLVLVNGeDLSRYREMSYKVTE 131
Cdd:PRK02406 2 DCFYAAVEMRDNPELRGKPVAVggspGRRGVISTCNYEARKFGVRSAMPTAQALKLCPDLIFVPG-RFDVYKEVSRQIRE 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226958460 132 LLEEFSPAVERLGFDENFVDLTEmvekrlqqlpseevpsvtvfghvynnqsvNLHNImhrrlvvGS--QIAAEMREAMYN 209
Cdd:PRK02406 81 IFRRYTDLIEPLSLDEAYLDVTD-----------------------------NKLCI-------GSatLIAQEIRQDIFE 124
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226958460 210 QLGLTGCAGVAPNKLLAKLVSGVFKPNQQTVLLPESCQHLIHSLNhIKEIPGIGYKTAKRLEVLGINSVHDLQTFPIKTL 289
Cdd:PRK02406 125 ELGLTASAGVAPNKFLAKIASDWNKPNGLFVITPEEVDAFLATLP-VEKIPGVGKVTAEKLHALGIYTCADLQKYDLAEL 203
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226958460 290 EKELGiAIAQRIQQLSFGEDKSPVTPSGPPQSFSEEDTFKK-CSSEVEAKAKIEEL---LSSLLTRVcQDGRKPHTVRLV 365
Cdd:PRK02406 204 IRHFG-KFGRRLYERARGIDERPVKPDRERKSVGVERTFAEdLYDLEACLAELPRLaekLERRLERA-KPDKRIKTVGVK 281
|
....*.
gi 226958460 366 IrRYSD 371
Cdd:PRK02406 282 L-KFAD 286
|
|
| IMS |
pfam00817 |
impB/mucB/samB family; These proteins are involved in UV protection (Swiss). |
53-230 |
5.30e-46 |
|
impB/mucB/samB family; These proteins are involved in UV protection (Swiss).
Pssm-ID: 425885 [Multi-domain] Cd Length: 148 Bit Score: 160.82 E-value: 5.30e-46
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226958460 53 VDLDCFYAQVEMISNPELKDRPLGVQQKY---LVVTCNYEARKLGVRKLMNVRDAKEKCPQLVLVnGEDLSRYREMSYKV 129
Cdd:pfam00817 1 IDMDAFFASVELLRDPELKGKPVAVGGGNgrgIVAAASYEARKYGVRSGMPVFEAKKLCPNLIVV-PPDLELYRRASRKI 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226958460 130 TELLEEF-SPAVERLGFDENFVDLTEMVEKrlqqlpseevpsvtvFGHVynnqsvnlhnimhrrlvvgSQIAAEMREAMY 208
Cdd:pfam00817 80 FEILRRFsTPKVEQASIDEAFLDLTGLEKL---------------FGAE-------------------EALAKRLRREIA 125
|
170 180
....*....|....*....|..
gi 226958460 209 NQLGLTGCAGVAPNKLLAKLVS 230
Cdd:pfam00817 126 EETGLTCSIGIAPNKLLAKLAS 147
|
|
| Rev1_UBM2 |
cd19318 |
Ubiquitin-Binding Motif 2 (UBM2) of Y-family polymerase Rev1; This model characterizes UBM2, ... |
699-727 |
7.96e-03 |
|
Ubiquitin-Binding Motif 2 (UBM2) of Y-family polymerase Rev1; This model characterizes UBM2, the second ubiquitin-binding motif of Rev1, a DNA damage tolerance protein. Rev1 acts as a translesion synthesis (TLS) DNA polymerase and may also recruit other TLS polymerases to the site of DNA damage; in that process the UBMs are essential for Rev1 function, triggering TLS activation via recognition of ubiquitin moieties in PCNA, the proliferating cell nuclear antigen.
Pssm-ID: 412037 Cd Length: 36 Bit Score: 34.51 E-value: 7.96e-03
10 20
....*....|....*....|....*....
gi 226958460 699 LPFPPDIDPQVFYELPEEVQKELMAEWER 727
Cdd:cd19318 6 LPSFSQVDPSVLAALPPDLQEELEAAYAQ 34
|
|
| |
|
Name |
Accession |
Description |
Interval |
E-value |
| PolY_Pol_iota |
cd01703 |
DNA Polymerase iota; Pol iota, also called Rad30B, is a translesion synthesis (TLS) polymerase. ... |
51-431 |
1.18e-174 |
|
DNA Polymerase iota; Pol iota, also called Rad30B, is a translesion synthesis (TLS) polymerase. Translesion synthesis is a process that allows the bypass of a variety of DNA lesions. TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. Pol iota is thought to be one of the least efficient polymerases, particularly when opposite pyrimidines; it can incorporate the correct nucleotide opposite a purine much more efficiently than opposite a pyrimidine, and prefers to insert guanosine instead of adenosine opposite thymidine. Pol iota is believed to use Hoogsteen rather than Watson-Crick base pairing, which may explain the varying efficiency for different template nucleotides.
Pssm-ID: 176457 [Multi-domain] Cd Length: 379 Bit Score: 505.08 E-value: 1.18e-174
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226958460 51 VHVDLDCFYAQVEMISNPELKDRPLGVQQKYLVVTCNYEARKLGVRKLMNVRDAKEKCPQLVLVNGEDLSRYREMSYKVT 130
Cdd:cd01703 1 IHLDLDCFYAQVEEIRDPSLKSKPLGIQQKYIVVTCNYEARRLGVKKLMSIKDAKEICPDLVLVNGEDLTPFRDMSKKVY 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226958460 131 ELLEEFSPA--VERLGFDENFVDLTEMvekrlqqlpseevpsvtvfghvynnqsvnlhnimhrRLVVGSQIAAEMREAMY 208
Cdd:cd01703 81 RLLRSYSWNdrVERLGFDENFMDVTEM------------------------------------RLLVASHIAYEMRERIE 124
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226958460 209 NQLGLTGCAGVAPNKLLAKLVSGVFKPNQQTVLLPESCQHLI--HSLNHIKEIPGIGYKTAKRLEVLGINSVHDLQTFPI 286
Cdd:cd01703 125 NELGLTCCAGIASNKLLAKLVGSVNKPNQQTTLLPPSCADLMdfMDLHDLRKIPGIGYKTAAKLEAHGISSVRDLQEFSN 204
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226958460 287 ---------------KTLEKELGIAIAQRIQQLSFGEDKSPV-TPSGPPQSFSEEDTFKKCSSEV--EAKAKIEELLSSL 348
Cdd:cd01703 205 rnrqtvgaapsllelLLMVKEFGEGIGQRIWKLLFGRDTSPVkPASDFPQQISIEDSYKKCSLEEirEARNKIEELLASL 284
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226958460 349 LTRVCQ--------DGRKPHTVRLVIRRYSD--KHCNRESRQCPIPSHVIQKLGTGNHDSMPPLIDILMKLFRNMVNVK- 417
Cdd:cd01703 285 LERMKQdlqevkagDGRRPHTLRLTLRRYTStkKHYNRESKQAPIPSHVFQKLTGGNEIAARPIEKILMRLFRELVPPKn 364
|
410
....*....|....*
gi 226958460 418 -MPFHLTLMSVCFCN 431
Cdd:cd01703 365 vKGFNLTLLNVCFTN 379
|
|
| PolY |
cd00424 |
Y-family of DNA polymerases; Y-family DNA polymerases are a specialized subset of polymerases ... |
51-430 |
3.58e-115 |
|
Y-family of DNA polymerases; Y-family DNA polymerases are a specialized subset of polymerases that facilitate translesion synthesis (TLS), a process that allows the bypass of a variety of DNA lesions. Unlike replicative polymerases, TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. The active sites of TLS polymerases are large and flexible to allow the accomodation of distorted bases. Most TLS polymerases are members of the Y-family, including Pol eta, Pol kappa/IV, Pol iota, Rev1, and Pol V, which is found exclusively in bacteria. In eukaryotes, the B-family polymerase Pol zeta also functions as a TLS polymerase. Expression of Y-family polymerases is often induced by DNA damage and is believed to be highly regulated. TLS is likely induced by the monoubiquitination of the replication clamp PCNA, which provides a scaffold for TLS polymerases to bind in order to access the lesion. Because of their high error rates, TLS polymerases are potential targets for cancer treatment and prevention.
Pssm-ID: 176453 [Multi-domain] Cd Length: 343 Bit Score: 350.89 E-value: 3.58e-115
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226958460 51 VHVDLDCFYAQVEMISNPELKDRPLGVQQKY----LVVTCNYEARKLGVRKLMNVRDAKEKCPQLVLVNgEDLSRYREMS 126
Cdd:cd00424 1 LHIDFDNFFASVEQLARPELKGRPVVVVPFNsdstCVIACSYEARKYGVKRGMPVREARKMCPNLILVP-ARLDLYRRLS 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226958460 127 YKVTELLEEFSPAVERLGFDENFVDLTEMVekrlqqlpseevpsvtvfghvynnqsvnlhnimhRRLVVGSQIAAEMREA 206
Cdd:cd00424 80 ERLLSELEEVAPLVEVASIDELFLDLTGSA----------------------------------RLLGLGSEVALRIKRH 125
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226958460 207 MYNQLG-LTGCAGVAPNKLLAKLVSGVFKPNQQTVLLPESCQHLIhSLNHIKEIPGIGYKTAKRLEVLGINSVHDLQTFP 285
Cdd:cd00424 126 IAEQLGgITASIGIASNKLLAKLAAKYAKPDGLTILDPEDLPGFL-SKLPLTDLPGIGAVTAKRLEAVGINPIGDLLAAS 204
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226958460 286 IKTLEKELGIAIAQRIQQLSFGEDKSPVTPSGPPQSFSEEDTFKKCSSEVE-AKAKIEELLSSLLTRVCQDGRKPHTVRL 364
Cdd:cd00424 205 PDALLALWGGVSGERLWYALRGIDDEPLSPPRPRKSFSHERVLPRDSRNAEdARPLLRLLLEKLARRLRRDGRGATRLRL 284
|
330 340 350 360 370 380
....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 226958460 365 VIRRYSD---KHCNRESRQCPIPSHViqklgtgnhdSMPPLIDILMKLFRNMVNVKMPFHLTLMSVCFC 430
Cdd:cd00424 285 WLRTVDGrwsGHADIPSRSAPRPIST----------EDGELLHALDKLWRALLDDKGPRRLRRLGVRLS 343
|
|
| DinP |
COG0389 |
Nucleotidyltransferase/DNA polymerase DinP involved in DNA repair [Replication, recombination ... |
48-422 |
2.23e-82 |
|
Nucleotidyltransferase/DNA polymerase DinP involved in DNA repair [Replication, recombination and repair];
Pssm-ID: 440158 [Multi-domain] Cd Length: 336 Bit Score: 265.08 E-value: 2.23e-82
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226958460 48 RVIVHVDLDCFYAQVEMISNPELKDRPLGV---QQKYLVVTCNYEARKLGVRKLMNVRDAKEKCPQLVLVNGeDLSRYRE 124
Cdd:COG0389 1 RRILHVDMDAFYASVEQRDRPELRGKPVAVggdNNRGVVAAASYEARAFGVRSGMPLFQARRLCPDLVVLPP-DFELYRD 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226958460 125 MSYKVTELLEEFSPAVERLGFDENFVDLTEMveKRLqqLPSEEvpsvtvfghvynnqsvnlhnimhrrlvvgsQIAAEMR 204
Cdd:COG0389 80 VSRRVMAILERYTPLVEPLSIDEAFLDVTGS--ARL--FGSAE------------------------------AIARRIR 125
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226958460 205 EAMYNQLGLTGCAGVAPNKLLAKLVSGVFKPNQQTVLLPESCQHLIHSLnHIKEIPGIGYKTAKRLEVLGINSVHDLQTF 284
Cdd:COG0389 126 RRIRRETGLTVSVGIAPNKFLAKIASDLAKPDGLTVIPPGEVAAFLAPL-PVEKLWGVGPKTAEKLARLGIRTIGDLAAL 204
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226958460 285 PIKTLEKELGIAiAQRIQQLSFGEDKSPVTPSGPPQSFSEEDTFKKcssEVEAKAKIEELLSSLLTRVC----QDGRKPH 360
Cdd:COG0389 205 PRAELRRRFGKV-GERLYRLARGIDPRPVEPRRPRKSIGVERTFGE---DLTDLEELEAALRRLAERLAerlrRQGLGAR 280
|
330 340 350 360 370 380
....*....|....*....|....*....|....*....|....*....|....*....|..
gi 226958460 361 TVRLVIrRYSDKHCNRESRQCPIPShviqklgtgnhDSMPPLIDILMKLFRNMVNVKMPFHL 422
Cdd:COG0389 281 TVTVKL-RTSDFRTTTRSRTLPEPT-----------DDTAELLRAARELLERIYRPGRPVRL 330
|
|
| PolY_Pol_IV_kappa |
cd03586 |
DNA Polymerase IV/Kappa; Pol IV, also known as Pol kappa, DinB, and Dpo4, is a translesion ... |
51-391 |
1.63e-81 |
|
DNA Polymerase IV/Kappa; Pol IV, also known as Pol kappa, DinB, and Dpo4, is a translesion synthesis (TLS) polymerase. Translesion synthesis is a process that allows the bypass of a variety of DNA lesions. TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. Known primarily as Pol IV in prokaryotes and Pol kappa in eukaryotes, this polymerase has a propensity for generating frameshift mutations. The eukaryotic Pol kappa differs from Pol IV and Dpo4 by an N-terminal extension of ~75 residues known as the "N-clasp" region. The structure of Pol kappa shows DNA that is almost totally encircled by Pol kappa, with the N-clasp region augmenting the interactions between DNA and the polymerase. Pol kappa is more resistant than Pol eta and Pol iota to bulky guanine adducts and is efficient at catalyzing the incorporation of dCTP. Bacterial pol IV has a higher error rate than other Y-family polymerases.
Pssm-ID: 176459 [Multi-domain] Cd Length: 334 Bit Score: 262.84 E-value: 1.63e-81
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226958460 51 VHVDLDCFYAQVEMISNPELKDRPLGV---QQKYLVVTCNYEARKLGVRKLMNVRDAKEKCPQLVLVNGeDLSRYREMSY 127
Cdd:cd03586 1 IHIDMDAFYASVEQRDNPELKGKPVAVggsSDRGVVSTASYEARKFGVRSAMPIFQAKKLCPNLIFVPP-RFDKYREVSR 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226958460 128 KVTELLEEFSPAVERLGFDENFVDLTEmvekrlqqlpseevpsvtvfgHVYNNQSvnlhnimhrrlvvGSQIAAEMREAM 207
Cdd:cd03586 80 QIMEILREYTPLVEPLSIDEAYLDVTD---------------------YVRLFGS-------------ATEIAKEIRARI 125
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226958460 208 YNQLGLTGCAGVAPNKLLAKLVSGVFKPNQQTVLLPESCQHLIHSLnHIKEIPGIGYKTAKRLEVLGINSVHDLQTFPIK 287
Cdd:cd03586 126 REETGLTASAGIAPNKFLAKIASDLNKPNGLTVIPPEDVEEFLAPL-PVRKIPGVGKVTAEKLKELGIKTIGDLAKLDVE 204
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226958460 288 TLEKELGiAIAQRIQQLSFGEDKSPVTPSGPPQSFSEEDTF-KKCSSEVEAKAKIEELLSSLLTRVCQDGRKPHTVRLVI 366
Cdd:cd03586 205 LLKKLFG-KSGRRLYELARGIDNRPVEPDRERKSIGVERTFsEDLTDPEELLEELLELAEELAERLRKRGLKGRTVTVKL 283
|
330 340
....*....|....*....|....*
gi 226958460 367 rRYSDKHCNRESRQCPIPSHVIQKL 391
Cdd:cd03586 284 -KYADFSTRTRSRTLPEPTDDAEDI 307
|
|
| PRK02406 |
PRK02406 |
DNA polymerase IV; Validated |
56-371 |
9.48e-64 |
|
DNA polymerase IV; Validated
Pssm-ID: 235035 [Multi-domain] Cd Length: 343 Bit Score: 216.14 E-value: 9.48e-64
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226958460 56 DCFYAQVEMISNPELKDRPLGV----QQKYLVVTCNYEARKLGVRKLMNVRDAKEKCPQLVLVNGeDLSRYREMSYKVTE 131
Cdd:PRK02406 2 DCFYAAVEMRDNPELRGKPVAVggspGRRGVISTCNYEARKFGVRSAMPTAQALKLCPDLIFVPG-RFDVYKEVSRQIRE 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226958460 132 LLEEFSPAVERLGFDENFVDLTEmvekrlqqlpseevpsvtvfghvynnqsvNLHNImhrrlvvGS--QIAAEMREAMYN 209
Cdd:PRK02406 81 IFRRYTDLIEPLSLDEAYLDVTD-----------------------------NKLCI-------GSatLIAQEIRQDIFE 124
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226958460 210 QLGLTGCAGVAPNKLLAKLVSGVFKPNQQTVLLPESCQHLIHSLNhIKEIPGIGYKTAKRLEVLGINSVHDLQTFPIKTL 289
Cdd:PRK02406 125 ELGLTASAGVAPNKFLAKIASDWNKPNGLFVITPEEVDAFLATLP-VEKIPGVGKVTAEKLHALGIYTCADLQKYDLAEL 203
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226958460 290 EKELGiAIAQRIQQLSFGEDKSPVTPSGPPQSFSEEDTFKK-CSSEVEAKAKIEEL---LSSLLTRVcQDGRKPHTVRLV 365
Cdd:PRK02406 204 IRHFG-KFGRRLYERARGIDERPVKPDRERKSVGVERTFAEdLYDLEACLAELPRLaekLERRLERA-KPDKRIKTVGVK 281
|
....*.
gi 226958460 366 IrRYSD 371
Cdd:PRK02406 282 L-KFAD 286
|
|
| PolY_Pol_eta |
cd01702 |
DNA Polymerase eta; Pol eta, also called Rad30A, is a translesion synthesis (TLS) polymerase. ... |
51-431 |
1.02e-56 |
|
DNA Polymerase eta; Pol eta, also called Rad30A, is a translesion synthesis (TLS) polymerase. Translesion synthesis is a process that allows the bypass of a variety of DNA lesions. TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. Unlike other Y-family members, Pol eta can efficiently and accurately replicate DNA past UV-induced lesions. Its activity is initiated by two simultaneous interactions: the PIP box in pol eta interacting with PCNA, and the UBZ (ubiquitin-binding zinc finger) in pol eta interacting with monoubiquitin attached to PCNA. Pol eta is more efficient in copying damaged DNA than undamaged DNA and seems to recognize when a lesion has been passed, facilitating a lesion-dependent dissociation from the DNA.
Pssm-ID: 176456 [Multi-domain] Cd Length: 359 Bit Score: 197.54 E-value: 1.02e-56
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226958460 51 VHVDLDCFYAQVEMISNPELKDRPLGVQQKYLVVTCNYEARKLGVRKLMNVRDAKEKCPQLVLV------NGED------ 118
Cdd:cd01702 1 AHIDMDAFFAQVEQVRLGLLRNDPVAVVQWNSIIAVSYAARAFGVTRFMTIDEAKKKCPDLILAhvatykKGEDeadyhe 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226958460 119 ----------LSRYREMSYKVTELLEEFSPAVERLGFDENFVDLtemvekrlqqlpseevpsvtvfghvynnqsvnlhni 188
Cdd:cd01702 81 npsparhkvsLDPYRRASRKILNILKRFGDVVEKASIDEAYLDL------------------------------------ 124
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226958460 189 mhrrlvvGSQIAAEMREAMYNQLGLTGCAGVAPNKLLAKLVSGVFKPNQQTVLLPESCQHLIHSLNhIKEIPGIGYKTAK 268
Cdd:cd01702 125 -------GSRIVEEIRQQVYDELGYTCSAGIAHNKMLAKLASGMNKPNAQTILRNDAVASFLSSLP-ITSIRGLGGKLGE 196
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226958460 269 RL-EVLGINSVHDLQTFPIKT--LEKELGIAIAQRIQQLSFGEDKSPVTPSGPPQSFSEEDTF--KKCSSEVEAKAKIEE 343
Cdd:cd01702 197 EIiDLLGLPTEGDVAGFRSSEsdLQEHFGEKLGEWLYNLLRGIDHEPVKPRPLPKSMGSSKNFpgKTALSTEDVQHWLLV 276
|
330 340 350 360 370 380 390 400
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226958460 344 LLSSLLTRVCQD----GRKPHTVRLVIRRYSDKhcNRESRQCPIPSHVIQKLgtgNHDSMppliDILMKLFRNMVNVKMP 419
Cdd:cd01702 277 LASELNSRLEDDryenNRRPKTLVLSLRQRGDG--VRRSRSCALPRYDAQKI---VKDAF----KLIKAINEEGLGLAWN 347
|
410
....*....|..
gi 226958460 420 FHLTLMSVCFCN 431
Cdd:cd01702 348 YPLTLLSLSFTK 359
|
|
| PRK01810 |
PRK01810 |
DNA polymerase IV; Validated |
47-371 |
9.47e-53 |
|
DNA polymerase IV; Validated
Pssm-ID: 179337 [Multi-domain] Cd Length: 407 Bit Score: 187.93 E-value: 9.47e-53
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226958460 47 SRVIVHVDLDCFYAQVEMISNPELKDRPLGV-----QQKYLVVTCNYEARKLGVRKLMNVRDAKEKCPQLVLVNgEDLSR 121
Cdd:PRK01810 4 GRVIFHVDMNSFFASVEIAYDPSLQGKPLAVagnekERKGIIVTCSYEARAYGIRTTMPLWEAKRLCPQLIVRR-PNFDR 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226958460 122 YREMSYKVTELLEEFSPAVERLGFDENFVDLTEMVEKrlqqlpseevpsvtvfGHVYnnqsvnlhnimhrrlvvgsQIAA 201
Cdd:PRK01810 83 YREASRQMFQILSEFTPLVQPVSIDEGYLDITDCYAL----------------GSPL-------------------EIAK 127
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226958460 202 EMREAMYNQLGLTGCAGVAPNKLLAKLVSGVFKPNQQTVLLPESCQHLIHSLnHIKEIPGIGYKTAKRLEVLGINSVHDL 281
Cdd:PRK01810 128 MIQQRLLTELQLPCSIGIAPNKFLAKMASDMKKPLGITVLRKRDVPEMLWPL-PVGEMHGIGEKTAEKLKDIGIQTIGDL 206
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226958460 282 QTFPIKTLEKELGIAiAQRIQQLSFGEDKSPVTPS--------GPPQSFSEE--------DTFKKCSSEVEAKAKIEELL 345
Cdd:PRK01810 207 AKADEHILRAKLGIN-GVRLQRRANGIDDRPVDPEaiyqfksvGNSTTLSHDmdeekellDVLRRLSKSVSKRLQKKTVV 285
|
330 340
....*....|....*....|....*.
gi 226958460 346 SSlltrvcqdgrkphTVRLVIrRYSD 371
Cdd:PRK01810 286 SY-------------NVQIMI-RYHD 297
|
|
| IMS |
pfam00817 |
impB/mucB/samB family; These proteins are involved in UV protection (Swiss). |
53-230 |
5.30e-46 |
|
impB/mucB/samB family; These proteins are involved in UV protection (Swiss).
Pssm-ID: 425885 [Multi-domain] Cd Length: 148 Bit Score: 160.82 E-value: 5.30e-46
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226958460 53 VDLDCFYAQVEMISNPELKDRPLGVQQKY---LVVTCNYEARKLGVRKLMNVRDAKEKCPQLVLVnGEDLSRYREMSYKV 129
Cdd:pfam00817 1 IDMDAFFASVELLRDPELKGKPVAVGGGNgrgIVAAASYEARKYGVRSGMPVFEAKKLCPNLIVV-PPDLELYRRASRKI 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226958460 130 TELLEEF-SPAVERLGFDENFVDLTEMVEKrlqqlpseevpsvtvFGHVynnqsvnlhnimhrrlvvgSQIAAEMREAMY 208
Cdd:pfam00817 80 FEILRRFsTPKVEQASIDEAFLDLTGLEKL---------------FGAE-------------------EALAKRLRREIA 125
|
170 180
....*....|....*....|..
gi 226958460 209 NQLGLTGCAGVAPNKLLAKLVS 230
Cdd:pfam00817 126 EETGLTCSIGIAPNKLLAKLAS 147
|
|
| PRK03348 |
PRK03348 |
DNA polymerase IV; Provisional |
46-369 |
3.09e-44 |
|
DNA polymerase IV; Provisional
Pssm-ID: 235118 [Multi-domain] Cd Length: 454 Bit Score: 165.49 E-value: 3.09e-44
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226958460 46 SSRVIVHVDLDCFYAQVEMISNPELKDRPL---GVQQKYLVVTCNYEARKLGVRKLMNVRDAKEKCPQLVLVNGEDLSRY 122
Cdd:PRK03348 3 AQRWVLHLDMDAFFASVEQLTRPTLRGRPVlvgGLGGRGVVAGASYEARVFGARSAMPMHQARRLVGNGAVVLPPRFVVY 82
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226958460 123 REMSYKVTELLEEFSPAVERLGFDENFVDLTEmvekrLQQLPSEEVpsvtvfghvynnqsvnlhnimhrrlvvgSQIAAE 202
Cdd:PRK03348 83 RAASRRVFDTLRELSPVVEQLSFDEAFVEPAE-----LAGASAEEV----------------------------EAFAER 129
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226958460 203 MREAMYNQLGLTGCAGVAPNKLLAKLVSGVFKPNQQTVLLPESCQHLIHSLNhIKEIPGIGYKTAKRLEVLGINSVHDLQ 282
Cdd:PRK03348 130 LRARVREETGLPASVGAGSGKQIAKIASGLAKPDGIRVVPPGEERELLAPLP-VRRLWGIGPVTEEKLHRLGIETIGDLA 208
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226958460 283 TFPIKTLEKELGIAIAQRIQQLSFGEDKSPVTPSGPPQSFSEEDTFKK-CSSEVEAKAKIEELLSSLLTRVCQDGRKPHT 361
Cdd:PRK03348 209 ALSEAEVANLLGATVGPALHRLARGIDDRPVAERAEAKQISAESTFAVdLTTRAQLREAIERIAEHAHRRLLKDGRGART 288
|
....*...
gi 226958460 362 VRLVIRRY 369
Cdd:PRK03348 289 VTVKLRKS 296
|
|
| PolY_Rev1 |
cd01701 |
DNA polymerase Rev1; Rev1 is a translesion synthesis (TLS) polymerase found in eukaryotes. ... |
44-385 |
2.22e-42 |
|
DNA polymerase Rev1; Rev1 is a translesion synthesis (TLS) polymerase found in eukaryotes. Translesion synthesis is a process that allows the bypass of a variety of DNA lesions. TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. Rev1 has both structural and enzymatic roles. Structurally, it is believed to interact with other nonclassical polymerases and replication machinery to act as a scaffold. Enzymatically, it catalyzes the specific insertion of dCMP opposite abasic sites. Rev1 interacts with the Rev7 subunit of the B-family TLS polymerase Pol zeta (Rev3/Rev7). Rev1 is known to actively promote the introduction of mutations, potentially making it a significant target for cancer treatment.
Pssm-ID: 176455 [Multi-domain] Cd Length: 404 Bit Score: 159.02 E-value: 2.22e-42
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226958460 44 ISSSRVIVHVDLDCFYAQVEMISNPELKDRPLGV-QQKYL---VVTCNYEARKLGVRKLMNVRDAKEKCPQLVLVnGEDL 119
Cdd:cd01701 43 PDLQRIIMHVDFDCFFVSVSIRNRPDLKGKPVAVcHGKGPnseIASCNYEARSYGIKNGMWVGQAKKLCPQLVTL-PYDF 121
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226958460 120 SRYREMSYKVTELLEEFSPAVERLGFDENFVDLTEmvekrlqqLPSEEVPSVTVFghvynnqsvnlhnimhrrlvvGSQI 199
Cdd:cd01701 122 EAYEEVSLTFYEILASYTDNIEAVSCDEALIDITS--------LLEETYELPEEL---------------------AEAI 172
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226958460 200 AAEMREAmynqlglTGC---AGVAPNKLLAKLVSGVFKPNQQTVLLPESCQHLIHSLNhIKEIPGIGYKTAKRLEVLGIN 276
Cdd:cd01701 173 RNEIRET-------TGCsasVGIGPNILLARLATRKAKPDGQYHLSAEKVEEFLSQLK-VGDLPGVGSSLAEKLVKLFGD 244
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226958460 277 SVHD--LQTFPIKTLEKELGIAIAQRIQQLSFGEDKSPVTPSGPPQSFSEEDT----FKKcssEVEAKAKIEELLSSLLT 350
Cdd:cd01701 245 TCGGleLRSKTKEKLQKVLGPKTGEKLYDYCRGIDDRPVTGEKERKSVSAEINygirFTN---VDDVEQFLQRLSEELSK 321
|
330 340 350 360
....*....|....*....|....*....|....*....|....*..
gi 226958460 351 RVCQDGRKPHTVRLVIRRYSDKH------------CNRESRQCPIPS 385
Cdd:cd01701 322 RLEESNVTGRQITLKLMKRAPGApieppkymghgiCDSFSKSSTLGV 368
|
|
| PRK02794 |
PRK02794 |
DNA polymerase IV; Provisional |
50-328 |
1.35e-39 |
|
DNA polymerase IV; Provisional
Pssm-ID: 179473 [Multi-domain] Cd Length: 419 Bit Score: 151.24 E-value: 1.35e-39
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226958460 50 IVHVDLDCFYAQVEMISNPELKDRPL--GVQQKYLVVTCNYEARKLGVRKLMNVRDAKEKCPQLVLVNGeDLSRYREMSY 127
Cdd:PRK02794 38 IAHIDCDAFYASVEKRDNPELRDKPViiGGGKRGVVSTACYIARIHGVRSAMPMFKALKLCPDAVVIKP-DMEKYVRVGR 116
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226958460 128 KVTELLEEFSPAVERLGFDENFVDL--TEmvekRLQQLPseevPSVTVfghvynnqsvnlhnimhrrlvvgSQIAAEMRE 205
Cdd:PRK02794 117 EVRAMMQALTPLVEPLSIDEAFLDLsgTE----RLHGAP----PAVVL-----------------------ARFARRVER 165
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226958460 206 amynQLGLTGCAGVAPNKLLAKLVSGVFKPNQQTVLLPESCQHLIHSlNHIKEIPGIGYKTAKRLEVLGINSVHDLQTFP 285
Cdd:PRK02794 166 ----EIGITVSVGLSYNKFLAKIASDLDKPRGFSVIGRAEALAFLAP-KPVGIIWGVGPATAARLARDGIRTIGDLQRAD 240
|
250 260 270 280
....*....|....*....|....*....|....*....|...
gi 226958460 286 IKTLEKELGiAIAQRIQQLSFGEDKSPVTPSGPPQSFSEEDTF 328
Cdd:PRK02794 241 EADLMRRFG-SMGLRLWRLARGIDDRKVSPDREAKSVSAETTF 282
|
|
| PRK14133 |
PRK14133 |
DNA polymerase IV; Provisional |
48-330 |
3.24e-39 |
|
DNA polymerase IV; Provisional
Pssm-ID: 184529 [Multi-domain] Cd Length: 347 Bit Score: 148.33 E-value: 3.24e-39
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226958460 48 RVIVHVDLDCFYAQVEMISNPELKDRPL---GVQQKYLVVTCNYEARKLGVRKLMNVRDAKEKCPQLVLVNGEdLSRYRE 124
Cdd:PRK14133 3 RVIIHVDMDAFFASVEQMDNPKLKGKPVivgGISERGVVSTCSYEARKYGVHSAMPVFMAKKRCPHGIFLPVR-HERYKE 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226958460 125 MSYKVTELLEEFSPAVERLGFDENFVDLTEMVEKrlqqlpseevpsvtvfghvynnqsvnlhnimhrrlvvGSQIAAEMR 204
Cdd:PRK14133 82 VSKNIFKILYEVTPIVEPVSIDEAYLDITNIKEE-------------------------------------PIKIAKYIK 124
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226958460 205 EAMYNQLGLTGCAGVAPNKLLAKLVSGVFKPNQQTVLLPESCQHLIHSLNhIKEIPGIGYKTAKRLEVLGINSVHDLQTF 284
Cdd:PRK14133 125 KKVKKETGLTLSVGISYNKFLAKLASDWNKPDGIKIITEDMIPDILKPLP-ISKVHGIGKKSVEKLNNIGIYTIEDLLKL 203
|
250 260 270 280
....*....|....*....|....*....|....*....|....*....
gi 226958460 285 PIKTLEK---ELGIAIAQRIQqlsfGEDKSPVTPSGPPQSFSEEDTFKK 330
Cdd:PRK14133 204 SREFLIEyfgKFGVEIYERIR----GIDYREVEVSRERKSIGKETTLKK 248
|
|
| PRK03103 |
PRK03103 |
DNA polymerase IV; Reviewed |
48-386 |
8.33e-37 |
|
DNA polymerase IV; Reviewed
Pssm-ID: 235104 [Multi-domain] Cd Length: 409 Bit Score: 143.22 E-value: 8.33e-37
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226958460 48 RVIVHVDLDCFYAQVEMISNPELKDRPLGV-----QQKYLVVTCNYEARKLGVRKLMNVRDAKEKCPQLVLVNGEdLSRY 122
Cdd:PRK03103 3 RVILLVDMQSFYASVEKAANPELKGRPVIVsgdpeRRSGVVLAACPLAKAYGVKTAERLWEAQQKCPDLVVVKPR-MQRY 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226958460 123 REMSYKVTELLEEFSPAVERLGFDENFVDLTEmvekrlqqlpseevpSVTVFGHVYnnqsvnlhnimhrrlvvgsQIAAE 202
Cdd:PRK03103 82 IDVSLQITRILEDFTDLVEPFSIDEQFLDVTG---------------SQKLFGSPL-------------------EIAQK 127
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226958460 203 MREAMYNQLGLTGCAGVAPNKLLAKLVSGVF---KPNQQTVLLPESCQHLIHSLNhIKEIPGIGYKTAKRLEVLGINSVH 279
Cdd:PRK03103 128 IQQRIMRETGVYARVGIGPNKLLAKMACDNFakkNPDGLFTLDKEDVPADLWPLP-VRKLFGVGSRMEKHLRRMGIRTIG 206
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226958460 280 DLQTFPIKTLEKELGIaIAQRIQQLSFGEDKSPVTPSGPP--QSFSEEDTFKKCSSEVEakaKIEELLSSLLTRVCQDGR 357
Cdd:PRK03103 207 QLANTPLERLKKRWGI-NGEVLWRTANGIDYSPVTPHSLDrqKAIGHQMTLPRDYRGFE---EIKVVLLELCEEVCRRAR 282
|
330 340 350
....*....|....*....|....*....|...
gi 226958460 358 KPH----TVRLVIRRYSDKHCNRESRQCPIPSH 386
Cdd:PRK03103 283 AKGymgrTVSVSLRGADFDWPTGFSRQMTLPEP 315
|
|
| PRK03858 |
PRK03858 |
DNA polymerase IV; Validated |
50-313 |
5.25e-36 |
|
DNA polymerase IV; Validated
Pssm-ID: 179663 [Multi-domain] Cd Length: 396 Bit Score: 140.51 E-value: 5.25e-36
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226958460 50 IVHVDLDCFYAQVEMISNPELKDRPL----GVqqkylVVTCNYEARKLGVRKLMNVRDAKEKCPQLVLVNGEdLSRYREM 125
Cdd:PRK03858 6 ILHADLDSFYASVEQRDDPALRGRPVivggGV-----VLAASYEAKAYGVRTAMGGRQARRLCPQAVVVPPR-MSAYSRA 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226958460 126 SYKVTELLEEFSPAVERLGFDENFVDLTEMveKRLQQLPseevpsvtvfghvynnqsvnlhnimhrrlvvgSQIAAEMRE 205
Cdd:PRK03858 80 SKAVFEVFRDTTPLVEGLSIDEAFLDVGGL--RRISGTP--------------------------------VQIAARLRR 125
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226958460 206 AMYNQLGLTGCAGVAPNKLLAKLVSGVFKPNQQTVLLPESCQHLIHSLNhIKEIPGIGYKTAKRLEVLGINSVHDLQTFP 285
Cdd:PRK03858 126 RVREEVGLPITVGVARTKFLAKVASQVAKPDGLLVVPPDRELAFLHPLP-VRRLWGVGPVTAAKLRAHGITTVGDVAELP 204
|
250 260
....*....|....*....|....*...
gi 226958460 286 IKTLEKELGIAIAQRIQQLSFGEDKSPV 313
Cdd:PRK03858 205 ESALVSLLGPAAGRHLHALAHNRDPRRV 232
|
|
| PolY_Pol_V_umuC |
cd01700 |
umuC subunit of DNA Polymerase V; umuC subunit of Pol V. Pol V is a bacterial translesion ... |
53-411 |
7.77e-36 |
|
umuC subunit of DNA Polymerase V; umuC subunit of Pol V. Pol V is a bacterial translesion synthesis (TLS) polymerase that consists of the heterotrimer of one umuC and two umuD subunits. Translesion synthesis is a process that allows the bypass of a variety of DNA lesions. TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. Pol V, RecA, single stranded DNA-binding protein, beta sliding clamp, and gamma clamp loading complex are responsible for inducing the SOS response in bacteria to repair UV-induced DNA damage.
Pssm-ID: 176454 [Multi-domain] Cd Length: 344 Bit Score: 138.45 E-value: 7.77e-36
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226958460 53 VDLDCFYAQVEMISNPELKDRPLGVqqkyL------VVTCNYEARKLGVRKLMNVRDAKEKCPQL-VLVNGEDLSRYREM 125
Cdd:cd01700 3 VDCNSFYASCERVFRPLLLGRPLVV----LsnndgcVIARSPEAKALGIKMGSPYFKVPDLLERHgVAVFSSNYALYGDM 78
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226958460 126 SYKVTELLEEFSPAVERLGFDENFVDLTEMvekrlqqlpseevpsvtvfghvynNQSVNLHnimhrrlvvgsQIAAEMRE 205
Cdd:cd01700 79 SRRIMSILERFSPDVEVYSIDESFLDLTGS------------------------LRFGDLE-----------ELARKIRR 123
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226958460 206 AMYNQLGLTGCAGVAPNKLLAKLVSGVFKPNQQT----VLLPESCQHLIHSLNHIKEIPGIGYKTAKRLEVLGINSVHDL 281
Cdd:cd01700 124 RILQETGIPVTVGIGPTKTLAKLANDLAKKKNPYggvvDLTDEEVRDKLLKILPVGDVWGIGRRTAKKLNAMGIHTAGDL 203
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226958460 282 QTFPIKTLEKELGIaIAQRIQQLSFGEDKSPVTPSGPP-QSFseedtfkkCSSE-----VEAKAKIEELLSSLLTRVC-- 353
Cdd:cd01700 204 AQADPDLLRKKFGV-VGERLVRELNGIDCLPLEEYPPPkKSI--------GSSRsfgrdVTDLDELKQALAEYAERAAek 274
|
330 340 350 360 370 380
....*....|....*....|....*....|....*....|....*....|....*....|
gi 226958460 354 --QDGRKPHTVRLVIRrysdkhCNRESRQCPIPSHViQKLGTGNHDSMppliDILMKLFR 411
Cdd:cd01700 275 lrRQKSVARTISVFIG------TSGFSRQPKYYSAT-NTLPYPTNDTR----EIVKAALR 323
|
|
| PRK01216 |
PRK01216 |
DNA polymerase IV; Validated |
49-315 |
1.16e-33 |
|
DNA polymerase IV; Validated
Pssm-ID: 179251 [Multi-domain] Cd Length: 351 Bit Score: 132.61 E-value: 1.16e-33
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226958460 49 VIVHVDLDCFYAQVEMISNPELKDRPLGV-------QQKYLVVTCNYEARKLGVRKLMNVRDAKEKCPQLVLVNGEDlSR 121
Cdd:PRK01216 2 IILFVDFDYFFAQVEEVLNPSLKGKPVVVcvysgrfEDSGAVATANYEARKLGIKAGMPIVEAKKILPNAVYLPMRK-EV 80
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226958460 122 YREMSYKVTELLEEFSPAVERLGFDENFVDLTEMVEkrlqqlpseevpsvtvfghvyNNQSvnlhnimhrrlvvGSQIAA 201
Cdd:PRK01216 81 YQQVSNRIMKLLREYSEKIEIASIDEAYLDISDKVK---------------------NYQD-------------AYNLGL 126
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226958460 202 EMREAMYNQLGLTGCAGVAPNKLLAKLVSGVFKPNQQTVLLPESCQHLIHSLNhIKEIPGIGYKTAKRLEVLGINSVHDL 281
Cdd:PRK01216 127 EIKNKILEKEKITVTVGISKNKVFAKIAADMAKPNGIKVIDDEEVKRFINELD-IADIPGIGDITAEKLKKLGVNKLVDT 205
|
250 260 270
....*....|....*....|....*....|....
gi 226958460 282 QTFPIKTLEKELGIAIAQRIQQLSFGEDKSPVTP 315
Cdd:PRK01216 206 LRIEFDELKGIIGEAKAKYLFSLARNEYNEPVRA 239
|
|
| PRK03352 |
PRK03352 |
DNA polymerase IV; Validated |
44-385 |
3.83e-33 |
|
DNA polymerase IV; Validated
Pssm-ID: 179564 [Multi-domain] Cd Length: 346 Bit Score: 130.91 E-value: 3.83e-33
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226958460 44 ISSSRVIVHVDLDCFYAQVEMISNPELKDRPLGV------QQKYLVVTC-NYEARKLGVRKLMNVRDAKEKCPQLVLVNg 116
Cdd:PRK03352 1 TAMPRWVLHVDLDQFIAAVELLRRPELAGLPVIVggngdpTEPRKVVTCaSYEARAFGVRAGMPLRTAARRCPDAVFLP- 79
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226958460 117 EDLSRYREMSYKVTELLEEFSPAVERLGFDENFVDLTemvekrlqqlpsEEVPsvtvfghvynnqsvnlhnimhrrlvvg 196
Cdd:PRK03352 80 SDPAAYDAASEEVMATLRDLGVPVEVWGWDEAFLGVD------------TDDP--------------------------- 120
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226958460 197 SQIAAEMREAMYNQLGLTGCAGVAPNKLLAKLVSGVFKPNQQTVLLPESCQHLIHSLNhIKEIPGIGYKTAKRLEVLGIN 276
Cdd:PRK03352 121 EALAEEIRAAVLERTGLSCSVGIGDNKLRAKIATGFAKPAGVFRLTDANWMAVMGDRP-TDALWGVGPKTAKRLAALGIT 199
|
250 260 270 280 290 300 310 320
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226958460 277 SVHDLQTFPIKTLEKELGIAIAQRIQQLSFGEDKSPVTpSGP--PQSFSEEDTFKK---CSSEVEakAKIEELLSSLLTR 351
Cdd:PRK03352 200 TVADLAAADPAELAATFGPTTGPWLLLLARGGGDTEVS-AEPwvPRSRSREVTFPQdltDRAEVE--SAVRELARRVLDE 276
|
330 340 350
....*....|....*....|....*....|....
gi 226958460 352 VCQDGRKPHTVRLVIrRYSDKHCNRESRQCPIPS 385
Cdd:PRK03352 277 VVAEGRPVTRVAVKV-RTATFYTRTKIRKLPEPT 309
|
|
| PTZ00205 |
PTZ00205 |
DNA polymerase kappa; Provisional |
51-281 |
4.08e-21 |
|
DNA polymerase kappa; Provisional
Pssm-ID: 140232 [Multi-domain] Cd Length: 571 Bit Score: 97.78 E-value: 4.08e-21
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226958460 51 VHVDLDCFYAQVEMISNPELKDRPLGVQQKYLVVTCNYEARKLGVRKLMNVRDAKEKCPQLVLVNgEDLSRYREMSYKVT 130
Cdd:PTZ00205 136 IHLDMDMFYAAVEIKKHPEYAAIPLAIGTMTMLQTANYVARGRGIRQGMPGFLALKICPNLLILP-PDFDAYNEESNTVR 214
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226958460 131 ELLEEFSPAVERLGFDENFVDLTEMVEKRLQQLPSEEVpsvtvfghvynnqsvnlhnimhrrlvvgsqiAAEMREAMYNQ 210
Cdd:PTZ00205 215 RIVAEYDPNYISFGLDELTLEVSAYIERFEGTKTAEDV-------------------------------ASELRVRVFGE 263
|
170 180 190 200 210 220 230
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 226958460 211 LGLTGCAGVAPNKLLAKLVSGVFKPNQQTVL---LPESCQHLIHSLNhIKEIPGIGYKTAKRLEVLGINSVHDL 281
Cdd:PTZ00205 264 TKLTASAGIGPTAALAKIASNINKPNGQHDLnlhTRGDVMTYVRDLG-LRSVPGVGKVTEALLKGLGITTLSDI 336
|
|
| umuC |
PRK03609 |
translesion error-prone DNA polymerase V subunit UmuC; |
52-308 |
5.02e-18 |
|
translesion error-prone DNA polymerase V subunit UmuC;
Pssm-ID: 179607 [Multi-domain] Cd Length: 422 Bit Score: 87.13 E-value: 5.02e-18
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226958460 52 HVDLDCFYAQVEMISNPELKDRPLGV--QQKYLVVTCNYEARKLGVR---KLMNVRDAKEKCPQLVLVNGEDLsrYREMS 126
Cdd:PRK03609 4 LCDVNSFYASCETVFRPDLRGKPVVVlsNNDGCVIARSAEAKALGIKmgdPWFKQKDLFRRCGVVCFSSNYEL--YADMS 81
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226958460 127 YKVTELLEEFSPAVERLGFDENFVDLTEMVEKRlqqlpseevpSVTVFGHvynnqsvnlhnimhrrlvvgsqiaaEMREA 206
Cdd:PRK03609 82 NRVMSTLEELSPRVEIYSIDEAFCDLTGVRNCR----------DLTDFGR-------------------------EIRAT 126
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226958460 207 MYNQLGLTGCAGVAPNKLLAKLVSGVFKP-NQQT---VLL--PESCQHLIhSLNHIKEIPGIGYKTAKRLEVLGINSVHD 280
Cdd:PRK03609 127 VLQRTHLTVGVGIAQTKTLAKLANHAAKKwQRQTggvVDLsnLERQRKLL-SLQPVEEVWGVGRRISKKLNAMGIKTALD 205
|
250 260
....*....|....*....|....*...
gi 226958460 281 LQTFPIKTLEKELGIAIAQRIQQLSfGE 308
Cdd:PRK03609 206 LADTNIRFIRKHFNVVLERTVRELR-GE 232
|
|
| PolY_like |
cd03468 |
DNA Polymerase Y-family; Y-family DNA polymerases are a specialized subset of polymerases that ... |
68-323 |
2.29e-11 |
|
DNA Polymerase Y-family; Y-family DNA polymerases are a specialized subset of polymerases that facilitate translesion synthesis (TLS), a process that allows the bypass of a variety of DNA lesions. Unlike replicative polymerases, TLS polymerases lack proofreading activity and have low fidelity and low processivity. They use damaged DNA as templates and insert nucleotides opposite the lesions. The active sites of TLS polymerases are large and flexible to allow the accomodation of distorted bases. Expression of Y-family polymerases is often induced by DNA damage and is believed to be highly regulated. TLS is likely induced by the monoubiquitination of the replication clamp PCNA, which provides a scaffold for TLS polymerases to bind in order to access the lesion. Because of their high error rates, TLS polymerases are potential targets for cancer treatment and prevention.
Pssm-ID: 176458 [Multi-domain] Cd Length: 335 Bit Score: 65.87 E-value: 2.29e-11
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226958460 68 PELKDRPLGV--QQKYLVVT-CNYEARKLGVRKLMNVRDAKEKCPQLVLVNgEDLSRYREMSYKVTELLEEFSPAVERLG 144
Cdd:cd03468 18 PADDEAPLAVveRKKAGRILaCNAAARAAGVRPGMPLAEALALCPNLQVVE-YDPEADARALQELALWLLRFTPLVALDG 96
|
90 100 110 120 130 140 150 160
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226958460 145 FDENFVDLTemvekrlqqlpseevPSVTVFGhvynnqsvnlhnimhrrlvvGSQIAAEMREAMYNQLGLTGCAGVAPNKL 224
Cdd:cd03468 97 PDGLLLDVT---------------GCLHLFG--------------------GEDALAASLRAALATLGLSARAGIADTPG 141
|
170 180 190 200 210 220 230 240
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226958460 225 LAKLVSgvFKPNQQTVLLPESCQHLIHSLNH-IKEIPGIGYKTAKRLEVLGINSVHDLQTFPIKTLEKELGIAIAQRIQQ 303
Cdd:cd03468 142 AAWLLA--RAGGGRGVLRREALAAALVLLAPlPVAALRLPPETVELLARLGLRTLGDLAALPRAELARRFGLALLLRLDQ 219
|
250 260
....*....|....*....|
gi 226958460 304 LsFGEDKSPVTPSGPPQSFS 323
Cdd:cd03468 220 A-YGRDPEPLLFSPPPPAFD 238
|
|
| IMS_C |
pfam11799 |
impB/mucB/samB family C-terminal domain; These proteins are involved in UV protection (Swiss). |
319-432 |
4.91e-08 |
|
impB/mucB/samB family C-terminal domain; These proteins are involved in UV protection (Swiss).
Pssm-ID: 463354 [Multi-domain] Cd Length: 104 Bit Score: 51.41 E-value: 4.91e-08
10 20 30 40 50 60 70 80
....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 226958460 319 PQSFSEEDTF-KKCSSEVEAKAKIEELLSSLLTRVCQDGRKPHTVRLVIrRYSDKHcnRESRQCPIPSHViqklgtgnhD 397
Cdd:pfam11799 1 RKSIGAERTFgRDLTDLEELREALLELAEELAERLRRQGLVARTVTVKI-RYSDFR--TITRSVTLPSPT---------D 68
|
90 100 110
....*....|....*....|....*....|....*
gi 226958460 398 SMPPLIDILMKLFRNMVNvkmPFHLTLMSVCFCNL 432
Cdd:pfam11799 69 DTDEIYRAALRLLRRLYR---GRPVRLLGVSLSNL 100
|
|
| IMS_HHH |
pfam11798 |
IMS family HHH motif; These proteins are involved in UV protection, eg. |
243-275 |
1.26e-05 |
|
IMS family HHH motif; These proteins are involved in UV protection, eg.
Pssm-ID: 432081 [Multi-domain] Cd Length: 32 Bit Score: 42.38 E-value: 1.26e-05
10 20 30
....*....|....*....|....*....|...
gi 226958460 243 PESCQHLIHSLNhIKEIPGIGYKTAKRLEVLGI 275
Cdd:pfam11798 1 PDDVPEFLWPLP-ISKIPGIGKKLAEKLKALGI 32
|
|
| HHH_5 |
pfam14520 |
Helix-hairpin-helix domain; |
258-301 |
8.02e-04 |
|
Helix-hairpin-helix domain;
Pssm-ID: 434010 [Multi-domain] Cd Length: 57 Bit Score: 37.85 E-value: 8.02e-04
10 20 30 40
....*....|....*....|....*....|....*....|....*.
gi 226958460 258 EIPGIGYKTAKRLEVLGINSVHDLQTFPIKTLEK--ELGIAIAQRI 301
Cdd:pfam14520 6 SISGIGPKTALALLSAGIGTVEDLAEADVDELAEipGIGEKTAQRI 51
|
|
| Rev1_UBM2 |
cd19318 |
Ubiquitin-Binding Motif 2 (UBM2) of Y-family polymerase Rev1; This model characterizes UBM2, ... |
699-727 |
7.96e-03 |
|
Ubiquitin-Binding Motif 2 (UBM2) of Y-family polymerase Rev1; This model characterizes UBM2, the second ubiquitin-binding motif of Rev1, a DNA damage tolerance protein. Rev1 acts as a translesion synthesis (TLS) DNA polymerase and may also recruit other TLS polymerases to the site of DNA damage; in that process the UBMs are essential for Rev1 function, triggering TLS activation via recognition of ubiquitin moieties in PCNA, the proliferating cell nuclear antigen.
Pssm-ID: 412037 Cd Length: 36 Bit Score: 34.51 E-value: 7.96e-03
10 20
....*....|....*....|....*....
gi 226958460 699 LPFPPDIDPQVFYELPEEVQKELMAEWER 727
Cdd:cd19318 6 LPSFSQVDPSVLAALPPDLQEELEAAYAQ 34
|
|
| |
