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Conserved domains on  [gi|21218438|ref|NP_065750|]
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transcriptional repressor p66-beta [Homo sapiens]

Protein Classification

transcriptional repressor p66A/B( domain architecture ID 11244100)

transcriptional repressor p66A and p66B includes the coiled-coil and interaction region (P66-CC) and a GATA zinc finger which uses four cysteine residues to coordinate a zinc ion, and bind to DNA

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
P66_CC pfam16563
Coiled-coil and interaction region of P66A and P66B with MBD2; This family is a short ...
 159-197 6.74e-14

Coiled-coil and interaction region of P66A and P66B with MBD2; This family is a short coiled-coil interaction region on the transcriptional repressors P66A and P66B. The P66A and B, or alpha and beta, complex with MBDs or methyl-binding domain-containing proteins via a coiled-coil region on each. This P66-MBD2 complex forms part of an assembly with NuRD, nucleosome remodelling and deacetylation protein. MBD2-NuRD binds methylated DNA and regulates transcription of eg, the foetal beta-globin gene during development.


:

Pssm-ID: 465178  Cd Length: 40  Bit Score: 65.90  E-value: 6.74e-14
                          10        20        30
                  ....*....|....*....|....*....|....*....
gi 21218438   159 IEERQQLIKQLRDELRLEEARLVLLKKLRQSQLQKENVV 197
Cdd:pfam16563   2 IEERERMIKKLREELRLEEAKLVLLKKLRQSQQQKENIV 40
GATA pfam00320
GATA zinc finger; This domain uses four cysteine residues to coordinate a zinc ion. This ...
 420-454 9.21e-09

GATA zinc finger; This domain uses four cysteine residues to coordinate a zinc ion. This domain binds to DNA. Two GATA zinc fingers are found in the GATA transcription factors. However there are several proteins which only contain a single copy of the domain.


:

Pssm-ID: 425605 [Multi-domain]  Cd Length: 36  Bit Score: 51.16  E-value: 9.21e-09
                          10        20        30
                  ....*....|....*....|....*....|....*.
gi 21218438   420 CAQCRTDFTPHWKQEKNGKI-LCEQCMTSNQKKALK 454
Cdd:pfam00320   1 CSNCGTTKTPLWRRGPNGNRtLCNACGLYYKKKGLK 36
 
Name Accession Description Interval E-value
P66_CC pfam16563
Coiled-coil and interaction region of P66A and P66B with MBD2; This family is a short ...
 159-197 6.74e-14

Coiled-coil and interaction region of P66A and P66B with MBD2; This family is a short coiled-coil interaction region on the transcriptional repressors P66A and P66B. The P66A and B, or alpha and beta, complex with MBDs or methyl-binding domain-containing proteins via a coiled-coil region on each. This P66-MBD2 complex forms part of an assembly with NuRD, nucleosome remodelling and deacetylation protein. MBD2-NuRD binds methylated DNA and regulates transcription of eg, the foetal beta-globin gene during development.


Pssm-ID: 465178  Cd Length: 40  Bit Score: 65.90  E-value: 6.74e-14
                          10        20        30
                  ....*....|....*....|....*....|....*....
gi 21218438   159 IEERQQLIKQLRDELRLEEARLVLLKKLRQSQLQKENVV 197
Cdd:pfam16563   2 IEERERMIKKLREELRLEEAKLVLLKKLRQSQQQKENIV 40
GATA pfam00320
GATA zinc finger; This domain uses four cysteine residues to coordinate a zinc ion. This ...
 420-454 9.21e-09

GATA zinc finger; This domain uses four cysteine residues to coordinate a zinc ion. This domain binds to DNA. Two GATA zinc fingers are found in the GATA transcription factors. However there are several proteins which only contain a single copy of the domain.


Pssm-ID: 425605 [Multi-domain]  Cd Length: 36  Bit Score: 51.16  E-value: 9.21e-09
                          10        20        30
                  ....*....|....*....|....*....|....*.
gi 21218438   420 CAQCRTDFTPHWKQEKNGKI-LCEQCMTSNQKKALK 454
Cdd:pfam00320   1 CSNCGTTKTPLWRRGPNGNRtLCNACGLYYKKKGLK 36
LIM4_PINCH cd09334
The fourth LIM domain of protein PINCH; The fourth LIM domain of protein PINCH: PINCH plays a ...
 406-443 2.40e-03

The fourth LIM domain of protein PINCH; The fourth LIM domain of protein PINCH: PINCH plays a pivotal role in the assembly of focal adhesions (FAs), regulating diverse functions in cell adhesion, growth, and differentiation through LIM-mediated protein-protein interactions. PINCH comprises an array of five LIM domains that interact with integrin-linked kinase (ILK), Nck2 (also called Nckbeta or Grb4) and other interaction partners. These interactions are essential for triggering the FA assembly and for relaying diverse mechanical and biochemical signals between Cell-extracellular matrix and the actin cytoskeleton. The PINCH LIM4 domain recognizes the third SH3 domain of another adaptor protein, Nck2. This step is an important component of integrin signaling event. LIM domains are 50-60 amino acids in size and share two characteristic zinc finger motifs. The two zinc fingers contain eight conserved residues, mostly cysteines and histidines, which coordinately bond to two zinc atoms. LIM domains function as adaptors or scaffolds to support the assem bly of multimeric protein complexes.


Pssm-ID: 188720 [Multi-domain]  Cd Length: 54  Bit Score: 36.18  E-value: 2.40e-03
                        10        20        30        40
                ....*....|....*....|....*....|....*....|.
gi 21218438 406 GKSCASLLR---VEPFVCAQCRTDFTPHWKQEKNGKILCEQ 443
Cdd:cd09334  12 GRVVTALGKhwhVEHFVCAKCEKPFLGHRHYEKKGLAYCET 52
 
Name Accession Description Interval E-value
P66_CC pfam16563
Coiled-coil and interaction region of P66A and P66B with MBD2; This family is a short ...
 159-197 6.74e-14

Coiled-coil and interaction region of P66A and P66B with MBD2; This family is a short coiled-coil interaction region on the transcriptional repressors P66A and P66B. The P66A and B, or alpha and beta, complex with MBDs or methyl-binding domain-containing proteins via a coiled-coil region on each. This P66-MBD2 complex forms part of an assembly with NuRD, nucleosome remodelling and deacetylation protein. MBD2-NuRD binds methylated DNA and regulates transcription of eg, the foetal beta-globin gene during development.


Pssm-ID: 465178  Cd Length: 40  Bit Score: 65.90  E-value: 6.74e-14
                          10        20        30
                  ....*....|....*....|....*....|....*....
gi 21218438   159 IEERQQLIKQLRDELRLEEARLVLLKKLRQSQLQKENVV 197
Cdd:pfam16563   2 IEERERMIKKLREELRLEEAKLVLLKKLRQSQQQKENIV 40
GATA pfam00320
GATA zinc finger; This domain uses four cysteine residues to coordinate a zinc ion. This ...
 420-454 9.21e-09

GATA zinc finger; This domain uses four cysteine residues to coordinate a zinc ion. This domain binds to DNA. Two GATA zinc fingers are found in the GATA transcription factors. However there are several proteins which only contain a single copy of the domain.


Pssm-ID: 425605 [Multi-domain]  Cd Length: 36  Bit Score: 51.16  E-value: 9.21e-09
                          10        20        30
                  ....*....|....*....|....*....|....*.
gi 21218438   420 CAQCRTDFTPHWKQEKNGKI-LCEQCMTSNQKKALK 454
Cdd:pfam00320   1 CSNCGTTKTPLWRRGPNGNRtLCNACGLYYKKKGLK 36
LIM pfam00412
LIM domain; This family represents two copies of the LIM structural domain.
 418-444 1.02e-03

LIM domain; This family represents two copies of the LIM structural domain.


Pssm-ID: 395333 [Multi-domain]  Cd Length: 57  Bit Score: 37.31  E-value: 1.02e-03
                          10        20
                  ....*....|....*....|....*..
gi 21218438   418 FVCAQCRTDFTPHWKQEKNGKILCEQC 444
Cdd:pfam00412  26 FRCAVCGKPLTTGDFYEKDGKLYCKHD 52
LIM4_PINCH cd09334
The fourth LIM domain of protein PINCH; The fourth LIM domain of protein PINCH: PINCH plays a ...
 406-443 2.40e-03

The fourth LIM domain of protein PINCH; The fourth LIM domain of protein PINCH: PINCH plays a pivotal role in the assembly of focal adhesions (FAs), regulating diverse functions in cell adhesion, growth, and differentiation through LIM-mediated protein-protein interactions. PINCH comprises an array of five LIM domains that interact with integrin-linked kinase (ILK), Nck2 (also called Nckbeta or Grb4) and other interaction partners. These interactions are essential for triggering the FA assembly and for relaying diverse mechanical and biochemical signals between Cell-extracellular matrix and the actin cytoskeleton. The PINCH LIM4 domain recognizes the third SH3 domain of another adaptor protein, Nck2. This step is an important component of integrin signaling event. LIM domains are 50-60 amino acids in size and share two characteristic zinc finger motifs. The two zinc fingers contain eight conserved residues, mostly cysteines and histidines, which coordinately bond to two zinc atoms. LIM domains function as adaptors or scaffolds to support the assem bly of multimeric protein complexes.


Pssm-ID: 188720 [Multi-domain]  Cd Length: 54  Bit Score: 36.18  E-value: 2.40e-03
                        10        20        30        40
                ....*....|....*....|....*....|....*....|.
gi 21218438 406 GKSCASLLR---VEPFVCAQCRTDFTPHWKQEKNGKILCEQ 443
Cdd:cd09334  12 GRVVTALGKhwhVEHFVCAKCEKPFLGHRHYEKKGLAYCET 52
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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