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Conserved domains on  [gi|14150155|ref|NP_115729|]
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vacuolar protein-sorting-associated protein 25 [Homo sapiens]

Protein Classification

vacuolar protein-sorting-associated protein 25( domain architecture ID 10531201)

vacuolar protein-sorting-associated protein 25 (VPS25) is a component of the ESCRT-II complex (endosomal sorting complex required for transport II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs

CATH:  1.10.10.570
Gene Ontology:  GO:0071985|GO:0000814|GO:0005198|GO:0042803
PubMed:  12194858|15469844
TCDB:  3.A.31

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
ESCRT-II pfam05871
ESCRT-II complex subunit; This family of conserved eukaryotic proteins are subunits of the ...
 10-139 1.43e-70

ESCRT-II complex subunit; This family of conserved eukaryotic proteins are subunits of the endosome associated complex ESCRT-II which recruits transport machinery for protein sorting at the multivesicular body (MVB). This protein complex transiently associates with the endosomal membrane and thereby initiates the formation of ESCRT-III, a membrane-associated protein complex that functions immediately downstream of ESCRT-II during sorting of MVB cargo. ESCRT-II in turn functions downstream of ESCRT-I, a protein complex that binds to ubiquitinated endosomal cargo.


:

Pssm-ID: 461763  Cd Length: 134  Bit Score: 209.72  E-value: 1.43e-70
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 14150155    10 QYRFPPFFTLQPNVDTRQKQLAAWCSLVLSFCRLHKQSSMTVMEA----QESPLFNNVKLQRKLPVESIQIVLEELRKKG 85
Cdd:pfam05871   1 IYSFPPFFTLQPNLETRKKQLQLWSDLILSYCRHHKIFRLSLSGAeaesLKSPLFNNKKINRRLSPEFIREILDELVKKG 80

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....
gi 14150155    86 NLEWLDKSKSSFLIMWRRPEEWGKLIYQWVSRSGQNNSVFTLYELTNGEDTEDE 139
Cdd:pfam05871  81 RAEWIDKTKTRCLIYWRTPEEWADLIYQWVEETGQLGTVLTLYELTEGDETRGE 134
 
Name Accession Description Interval E-value
ESCRT-II pfam05871
ESCRT-II complex subunit; This family of conserved eukaryotic proteins are subunits of the ...
 10-139 1.43e-70

ESCRT-II complex subunit; This family of conserved eukaryotic proteins are subunits of the endosome associated complex ESCRT-II which recruits transport machinery for protein sorting at the multivesicular body (MVB). This protein complex transiently associates with the endosomal membrane and thereby initiates the formation of ESCRT-III, a membrane-associated protein complex that functions immediately downstream of ESCRT-II during sorting of MVB cargo. ESCRT-II in turn functions downstream of ESCRT-I, a protein complex that binds to ubiquitinated endosomal cargo.


Pssm-ID: 461763  Cd Length: 134  Bit Score: 209.72  E-value: 1.43e-70
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 14150155    10 QYRFPPFFTLQPNVDTRQKQLAAWCSLVLSFCRLHKQSSMTVMEA----QESPLFNNVKLQRKLPVESIQIVLEELRKKG 85
Cdd:pfam05871   1 IYSFPPFFTLQPNLETRKKQLQLWSDLILSYCRHHKIFRLSLSGAeaesLKSPLFNNKKINRRLSPEFIREILDELVKKG 80

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....
gi 14150155    86 NLEWLDKSKSSFLIMWRRPEEWGKLIYQWVSRSGQNNSVFTLYELTNGEDTEDE 139
Cdd:pfam05871  81 RAEWIDKTKTRCLIYWRTPEEWADLIYQWVEETGQLGTVLTLYELTEGDETRGE 134
 
Name Accession Description Interval E-value
ESCRT-II pfam05871
ESCRT-II complex subunit; This family of conserved eukaryotic proteins are subunits of the ...
 10-139 1.43e-70

ESCRT-II complex subunit; This family of conserved eukaryotic proteins are subunits of the endosome associated complex ESCRT-II which recruits transport machinery for protein sorting at the multivesicular body (MVB). This protein complex transiently associates with the endosomal membrane and thereby initiates the formation of ESCRT-III, a membrane-associated protein complex that functions immediately downstream of ESCRT-II during sorting of MVB cargo. ESCRT-II in turn functions downstream of ESCRT-I, a protein complex that binds to ubiquitinated endosomal cargo.


Pssm-ID: 461763  Cd Length: 134  Bit Score: 209.72  E-value: 1.43e-70
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 14150155    10 QYRFPPFFTLQPNVDTRQKQLAAWCSLVLSFCRLHKQSSMTVMEA----QESPLFNNVKLQRKLPVESIQIVLEELRKKG 85
Cdd:pfam05871   1 IYSFPPFFTLQPNLETRKKQLQLWSDLILSYCRHHKIFRLSLSGAeaesLKSPLFNNKKINRRLSPEFIREILDELVKKG 80

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....
gi 14150155    86 NLEWLDKSKSSFLIMWRRPEEWGKLIYQWVSRSGQNNSVFTLYELTNGEDTEDE 139
Cdd:pfam05871  81 RAEWIDKTKTRCLIYWRTPEEWADLIYQWVEETGQLGTVLTLYELTEGDETRGE 134
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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