P21-Rho-binding domain; Small domains that bind Cdc42p- and/or Rho-like small GTPases. Also known as the Cdc42/Rac interactive binding (CRIB).

                          10        20        30        40        50        60
                  ....*....|....*....|....*....|....*....|....*....|....*....|..
gi 28572033   200 DISRPTNFVHLSHVGWDAQKGFDlagNENDEVLNEFFVKAGVSEVELKDRDTRAFIYDFIQS 261
Cdd:pfam00786   1 MISAPTNFKHTVHVGFDPDTGFF---TGLPPEWAKLLDSSGITEDEQKENPKAVLDVLKFYS 59

WASP family proteins EVH1 domain; The Wiskott-Aldrich Syndrome Protein (WASP; also called Bee1p) and its homolog N (neuronal)-WASP are signal transduction proteins that promote actin polymerization in response to upstream intracellular signals. WAS is an X-linked recessive disease, characterized by eczema, immunodeficiency, and thrombocytopenia. The majority of patients with WAS, or a milder version of the disorder, X-linked thrombocytopenia (XLT), have point mutations in the EVH1 domain of WASP. WASP is an actin regulatory protein consisting of an N-terminal EVH1 domain called WH1 which binds LPPPEP peptides, a basic region (B), a GTP binding domain (GBP), a proline rich region, a WH2 domain, and a verprolin-cofilin-acidic motif (VCA) which activates the actin-related protein (Arp)2/3 actin nucleating complex. The B, GBD, and the proline-rich region are involved in autoinhibitory interactions that repress or block the activity of the VCA. Yeast members lack the GTP binding domain. The EVH1 domains are part of the PH domain superamily. There are 5 EVH1 subfamilies: Enables/VASP, Homer/Vesl, WASP, Dcp1, and Spred. Ligands are known for three of the EVH1 subfamilies, all of which bind proline-rich sequences: the Enabled/VASP family binds to FPPPP peptides, the Homer/Vesl family binds PPxxF peptides, and the WASP family binds LPPPEP peptides. EVH1 has a PH-like fold, despite having minimal sequence similarity to PH or PTB domains.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28572033   7 TLNTAVVQIYKTEGSaHAHW-KKRHTGVVCFVKDSAIRSYFMRAYCLIKNELIWEHEIYDGMEVVKSRPFLLTFEGSDGH 85
Cdd:cd01205   1 ILAAAVARLYYAEPD-PDSWsYTGLTGALCFVKDNAKRSYFFRLVDLKTRGVVWEQELYEGFEYNQDRPFFHTFEGDECM 79

                        90       100
                ....*....|....*....|..
gi 28572033  86 VGLNFVSEEECDSFFRIVDATI 107
Cdd:cd01205  80 IGLNFADEDEAAAFYKKVQEKL 101

WASP homology region 1; Region of the Wiskott-Aldrich syndrome protein (WASp) that contains point mutations in the majority of patients with WAS. Unknown function. Ena-like WH1 domains bind polyproline-containing peptides, and that Homer contains a WH1 domain.

                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28572033      1 MRRKLKTLNTAVVQIYKTEgsaHAHWKKRHTG-VVCFVKDSAIRSYFMRAYCL-IKNELIWEHEIYDGMEVVKSRPFLLT 78
Cdd:smart00461   1 LGSQCIILARAVVQLYDAD---TKKWVPTGEGgAANLVIDKNQRSYFFRIVGIkGQDKVIWNQELYKNFKYNQATPTFHQ 77

                           90       100
                   ....*....|....*....|....*
gi 28572033     79 FEGSDGHVGLNFVSEEECDSFFRIV 103
Cdd:smart00461  78 WADDKCVYGLNFASEEEAKKFRKKV 102

P21-Rho-binding domain; Small domains that bind Cdc42p- and/or Rho-like small GTPases. Also known as the Cdc42/Rac interactive binding (CRIB).

                           10        20
                   ....*....|....*....|
gi 28572033    201 ISRPTNFVHLSHVGWDAQKG 220
Cdd:smart00285   1 ISTPTNFKHIAHVGFDGQTG 20

WASP family proteins EVH1 domain; The Wiskott-Aldrich Syndrome Protein (WASP; also called Bee1p) and its homolog N (neuronal)-WASP are signal transduction proteins that promote actin polymerization in response to upstream intracellular signals. WAS is an X-linked recessive disease, characterized by eczema, immunodeficiency, and thrombocytopenia. The majority of patients with WAS, or a milder version of the disorder, X-linked thrombocytopenia (XLT), have point mutations in the EVH1 domain of WASP. WASP is an actin regulatory protein consisting of an N-terminal EVH1 domain called WH1 which binds LPPPEP peptides, a basic region (B), a GTP binding domain (GBP), a proline rich region, a WH2 domain, and a verprolin-cofilin-acidic motif (VCA) which activates the actin-related protein (Arp)2/3 actin nucleating complex. The B, GBD, and the proline-rich region are involved in autoinhibitory interactions that repress or block the activity of the VCA. Yeast members lack the GTP binding domain. The EVH1 domains are part of the PH domain superamily. There are 5 EVH1 subfamilies: Enables/VASP, Homer/Vesl, WASP, Dcp1, and Spred. Ligands are known for three of the EVH1 subfamilies, all of which bind proline-rich sequences: the Enabled/VASP family binds to FPPPP peptides, the Homer/Vesl family binds PPxxF peptides, and the WASP family binds LPPPEP peptides. EVH1 has a PH-like fold, despite having minimal sequence similarity to PH or PTB domains.

                        10        20        30        40        50        60        70        80
                ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28572033   7 TLNTAVVQIYKTEGSaHAHW-KKRHTGVVCFVKDSAIRSYFMRAYCLIKNELIWEHEIYDGMEVVKSRPFLLTFEGSDGH 85
Cdd:cd01205   1 ILAAAVARLYYAEPD-PDSWsYTGLTGALCFVKDNAKRSYFFRLVDLKTRGVVWEQELYEGFEYNQDRPFFHTFEGDECM 79

                        90       100
                ....*....|....*....|..
gi 28572033  86 VGLNFVSEEECDSFFRIVDATI 107
Cdd:cd01205  80 IGLNFADEDEAAAFYKKVQEKL 101

WASP homology region 1; Region of the Wiskott-Aldrich syndrome protein (WASp) that contains point mutations in the majority of patients with WAS. Unknown function. Ena-like WH1 domains bind polyproline-containing peptides, and that Homer contains a WH1 domain.

                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 28572033      1 MRRKLKTLNTAVVQIYKTEgsaHAHWKKRHTG-VVCFVKDSAIRSYFMRAYCL-IKNELIWEHEIYDGMEVVKSRPFLLT 78
Cdd:smart00461   1 LGSQCIILARAVVQLYDAD---TKKWVPTGEGgAANLVIDKNQRSYFFRIVGIkGQDKVIWNQELYKNFKYNQATPTFHQ 77

                           90       100
                   ....*....|....*....|....*
gi 28572033     79 FEGSDGHVGLNFVSEEECDSFFRIV 103
Cdd:smart00461  78 WADDKCVYGLNFASEEEAKKFRKKV 102

PAK (p21 activated kinase) Binding Domain (PBD), binds Cdc42p- and/or Rho-like small GTPases; also known as the Cdc42/Rac interactive binding (CRIB) motif; has been shown to inhibit transcriptional activation and cell transformation mediated by the Ras-Rac pathway. CRIB-containing effector proteins are functionally diverse and include serine/threonine kinases, tyrosine kinases, actin-binding proteins, and adapter molecules.

                        10        20        30        40
                ....*....|....*....|....*....|....*....|....*
gi 28572033 199 ADISRPTNFVHLSHVGWDAQkGFDLAGneNDEVLNEFFVKAGVSE 243
Cdd:cd00132   1 MEISTPTDFKHISHVGWDGV-GFDGAN--LPPDLQSLFQTAGISA 42

P21-Rho-binding domain; Small domains that bind Cdc42p- and/or Rho-like small GTPases. Also known as the Cdc42/Rac interactive binding (CRIB).

                           10        20
                   ....*....|....*....|
gi 28572033    201 ISRPTNFVHLSHVGWDAQKG 220
Cdd:smart00285   1 ISTPTNFKHIAHVGFDGQTG 20