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Conserved domains on  [gi|568998130|ref|XP_006523338|]
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ribonuclease T2-A isoform X1 [Mus musculus]

Protein Classification

T2 family ribonuclease( domain architecture ID 10452025)

T2 family ribonuclease catalyzes a two-stage endonucleolytic cleavage of RNA to form 3'-nucleotides; similar to Danio rerio ribonuclease T2 that has ribonuclease activity, with higher activity at acidic pH

CATH:  3.90.730.10
EC:  4.6.1.19
Gene Ontology:  GO:0003723|GO:0033897
PubMed:  12109772
SCOP:  4001977

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
Ribonuclease_T2 pfam00445
Ribonuclease T2 family;
2-127 6.13e-48

Ribonuclease T2 family;


:

Pssm-ID: 459812  Cd Length: 181  Bit Score: 153.66  E-value: 6.13e-48
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568998130    2 DLLRDMKIYWPDVIHrsSNRSQFWKHEWVKHGTCAAQVdaLNSEKKYFGKSLDLYKQIDLNSVLQKFGIKPSIN-YYQLA 80
Cdd:pfam00445  62 DLLNDLNKYWPSLKS--GNGESFWKHEWEKHGTCASTS--LDDEHDYFNAALKLRKKLNLLSALASAGIVPSDTkTYTLS 137

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*..
gi 568998130   81 DFKDALTRIYGVVPKIQCLMPEQGEsvQTVGQIELCFTKeDLHLRNC 127
Cdd:pfam00445 138 DIKDALKKGFGGTPYIQCNRDPSGN--QQLYEIRLCFDK-GLTFIDC 181
 
Name Accession Description Interval E-value
Ribonuclease_T2 pfam00445
Ribonuclease T2 family;
2-127 6.13e-48

Ribonuclease T2 family;


Pssm-ID: 459812  Cd Length: 181  Bit Score: 153.66  E-value: 6.13e-48
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568998130    2 DLLRDMKIYWPDVIHrsSNRSQFWKHEWVKHGTCAAQVdaLNSEKKYFGKSLDLYKQIDLNSVLQKFGIKPSIN-YYQLA 80
Cdd:pfam00445  62 DLLNDLNKYWPSLKS--GNGESFWKHEWEKHGTCASTS--LDDEHDYFNAALKLRKKLNLLSALASAGIVPSDTkTYTLS 137

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*..
gi 568998130   81 DFKDALTRIYGVVPKIQCLMPEQGEsvQTVGQIELCFTKeDLHLRNC 127
Cdd:pfam00445 138 DIKDALKKGFGGTPYIQCNRDPSGN--QQLYEIRLCFDK-GLTFIDC 181
RNase_T2_euk cd01061
Ribonuclease T2 (RNase T2) is a widespread family of secreted RNases found in every organism ...
 2-131 6.58e-46

Ribonuclease T2 (RNase T2) is a widespread family of secreted RNases found in every organism examined thus far. This family includes RNase Rh, RNase MC1, RNase LE, and self-incompatibility RNases (S-RNases). Plant T2 RNases are expressed during leaf senescence in order to scavenge phosphate from ribonucleotides. They are also expressed in response to wounding or pathogen invasion. S-RNases are thought to prevent self-fertilization by acting as selective cytotoxins of "self" pollen. Generally, RNases have two distinct binding sites: the primary site (B1 site) and the subsite (B2 site), for nucleotides located at the 5'- and 3'- terminal ends of the sessil bond, respectively. This CD includes the eukaryotic RNase T2 family members.


Pssm-ID: 238512 [Multi-domain]  Cd Length: 195  Bit Score: 149.02  E-value: 6.58e-46
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568998130   2 DLLRDMKIYWPDVIHRSSNrSQFWKHEWVKHGTCAAqvDALNSEKKYFGKSLDLYKQIDLNSVLQKFGIKPSINYYQLAD 81
Cdd:cd01061   61 DLLNELNKYWPDLTGPKNN-QSFWEHEWNKHGTCSS--TLLYNQYDYFDTALKLKDKLDLLKILAKAGIVPSTQTYTLSD 137

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|
gi 568998130  82 FKDALTRIYGVVPKIQCLmpeQGESVQTVGQIELCFTKEDLHLRNCTEPG 131
Cdd:cd01061  138 IQNAIKAATGVTPVIKCS---KDPGKGELNEIWICFDKKGGEFIDCPRPP 184
RnaI COG3719
Ribonuclease I [Translation, ribosomal structure and biogenesis];
27-127 9.34e-06

Ribonuclease I [Translation, ribosomal structure and biogenesis];


Pssm-ID: 442933  Cd Length: 222  Bit Score: 44.19  E-value: 9.34e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568998130  27 HEWVKHGTCAAQvdalnSEKKYFGKSLDLYKQIDLNSVLQKfgikpsINYYQ---LADFKDALTRIYGVVP----KIQCl 99
Cdd:COG3719  113 HEWKKHGTCSGL-----SPDDYFALARRLREAVNIPAVGRA------LNIGKtvtAAEVEAAFDAANPGLApdaiAVTC- 180

                         90       100
                 ....*....|....*....|....*...
gi 568998130 100 mpEQGEsvqtVGQIELCFTKeDLHLRNC 127
Cdd:COG3719  181 --RRGR----LTEVRICLSK-DLKPRPC 201
 
Name Accession Description Interval E-value
Ribonuclease_T2 pfam00445
Ribonuclease T2 family;
2-127 6.13e-48

Ribonuclease T2 family;


Pssm-ID: 459812  Cd Length: 181  Bit Score: 153.66  E-value: 6.13e-48
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568998130    2 DLLRDMKIYWPDVIHrsSNRSQFWKHEWVKHGTCAAQVdaLNSEKKYFGKSLDLYKQIDLNSVLQKFGIKPSIN-YYQLA 80
Cdd:pfam00445  62 DLLNDLNKYWPSLKS--GNGESFWKHEWEKHGTCASTS--LDDEHDYFNAALKLRKKLNLLSALASAGIVPSDTkTYTLS 137

                          90       100       110       120
                  ....*....|....*....|....*....|....*....|....*..
gi 568998130   81 DFKDALTRIYGVVPKIQCLMPEQGEsvQTVGQIELCFTKeDLHLRNC 127
Cdd:pfam00445 138 DIKDALKKGFGGTPYIQCNRDPSGN--QQLYEIRLCFDK-GLTFIDC 181
RNase_T2_euk cd01061
Ribonuclease T2 (RNase T2) is a widespread family of secreted RNases found in every organism ...
 2-131 6.58e-46

Ribonuclease T2 (RNase T2) is a widespread family of secreted RNases found in every organism examined thus far. This family includes RNase Rh, RNase MC1, RNase LE, and self-incompatibility RNases (S-RNases). Plant T2 RNases are expressed during leaf senescence in order to scavenge phosphate from ribonucleotides. They are also expressed in response to wounding or pathogen invasion. S-RNases are thought to prevent self-fertilization by acting as selective cytotoxins of "self" pollen. Generally, RNases have two distinct binding sites: the primary site (B1 site) and the subsite (B2 site), for nucleotides located at the 5'- and 3'- terminal ends of the sessil bond, respectively. This CD includes the eukaryotic RNase T2 family members.


Pssm-ID: 238512 [Multi-domain]  Cd Length: 195  Bit Score: 149.02  E-value: 6.58e-46
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568998130   2 DLLRDMKIYWPDVIHRSSNrSQFWKHEWVKHGTCAAqvDALNSEKKYFGKSLDLYKQIDLNSVLQKFGIKPSINYYQLAD 81
Cdd:cd01061   61 DLLNELNKYWPDLTGPKNN-QSFWEHEWNKHGTCSS--TLLYNQYDYFDTALKLKDKLDLLKILAKAGIVPSTQTYTLSD 137

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|
gi 568998130  82 FKDALTRIYGVVPKIQCLmpeQGESVQTVGQIELCFTKEDLHLRNCTEPG 131
Cdd:cd01061  138 IQNAIKAATGVTPVIKCS---KDPGKGELNEIWICFDKKGGEFIDCPRPP 184
RNase_T2 cd00374
Ribonuclease T2 (RNase T2) is a widespread family of secreted RNases found in every organism ...
 2-131 1.29e-37

Ribonuclease T2 (RNase T2) is a widespread family of secreted RNases found in every organism examined thus far. This family includes RNase Rh, RNase MC1, RNase LE, and self-incompatibility RNases (S-RNases). Plant T2 RNases are expressed during leaf senescence in order to scavenge phosphate from ribonucleotides. They are also expressed in response to wounding or pathogen invasion. S-RNases are thought to prevent self-fertilization by acting as selective cytotoxins of "self" pollen.


Pssm-ID: 238220  Cd Length: 195  Bit Score: 127.57  E-value: 1.29e-37
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568998130   2 DLLRDMKIYWPDVIHrsSNRSQFWKHEWVKHGTCAAQvdaLNSEKKYFGKSLDLYKQIDLNSVLQKFGIKPSI-NYYQLA 80
Cdd:cd00374   62 DLLDELNKYWPDLMP--GKDSSFWKHEWNKHGTCSGT---LLDQDDYFRTALKLLDKLDLLSILAKAGIKPSDgSTYTLA 136

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|.
gi 568998130  81 DFKDALTRIYGVVPKIQClmpEQGESVQTVGQIELCFTKEDLHLRNCTEPG 131
Cdd:cd00374  137 FIQNAIKAATGATPSLKC---TKDPGKGLLTEIWICFDKDALKFIDCPTPG 184
RnaI COG3719
Ribonuclease I [Translation, ribosomal structure and biogenesis];
27-127 9.34e-06

Ribonuclease I [Translation, ribosomal structure and biogenesis];


Pssm-ID: 442933  Cd Length: 222  Bit Score: 44.19  E-value: 9.34e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568998130  27 HEWVKHGTCAAQvdalnSEKKYFGKSLDLYKQIDLNSVLQKfgikpsINYYQ---LADFKDALTRIYGVVP----KIQCl 99
Cdd:COG3719  113 HEWKKHGTCSGL-----SPDDYFALARRLREAVNIPAVGRA------LNIGKtvtAAEVEAAFDAANPGLApdaiAVTC- 180

                         90       100
                 ....*....|....*....|....*...
gi 568998130 100 mpEQGEsvqtVGQIELCFTKeDLHLRNC 127
Cdd:COG3719  181 --RRGR----LTEVRICLSK-DLKPRPC 201
RNase_T2_prok cd01062
Ribonuclease T2 (RNase T2) is a widespread family of secreted RNases found in every organism ...
 26-127 2.46e-04

Ribonuclease T2 (RNase T2) is a widespread family of secreted RNases found in every organism examined thus far. This family includes RNase Rh, RNase MC1, RNase LE, and self-incompatibility RNases (S-RNases). Plant T2 RNases are expressed during leaf senescence in order to scavenge phosphate from ribonucleotides. They are also expressed in response to wounding or pathogen invasion. S-RNases are thought to prevent self-fertilization by acting as selective cytotoxins of "self" pollen. Generally, RNases have two distinct binding sites: the primary site (B1 site) and the subsite (B2 site), for nucleotides located at the 5'- and 3'- terminal ends of the sessil bond, respectively. This CD includes the prokaryotic RNase T2 family members.


Pssm-ID: 238513  Cd Length: 184  Bit Score: 39.67  E-value: 2.46e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 568998130  26 KHEWVKHGTCAAQvdalnSEKKYFGKSLDLYKQIDLNSVLQKFgikPSINYYQLADFKDALTRIYGVVP----KIQClmp 101
Cdd:cd01062   80 RHEWRKHGTCSGL-----DPDAYFAKARNLREALKIPPELRLL---AGNIGVTASEIRQAFIKANPGLPpdavSVSC--- 148

                         90       100
                 ....*....|....*....|....*.
gi 568998130 102 eQGESVQtvgQIELCFTKEDLhLRNC 127
Cdd:cd01062  149 -QGGLLT---EVRICLDKDLK-FAAC 169
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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