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Conserved domains on  [gi|569010624|ref|XP_006528336|]
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deoxyribonuclease-1-like 1 isoform X2 [Mus musculus]

Protein Classification

exonuclease/endonuclease/phosphatase family protein( domain architecture ID 662)

exonuclease/endonuclease/phosphatase (EEP) family protein may cleave phosphodiester bonds

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
EEP super family cl00490
Exonuclease-Endonuclease-Phosphatase (EEP) domain superfamily; This large superfamily includes ...
 27-191 1.20e-72

Exonuclease-Endonuclease-Phosphatase (EEP) domain superfamily; This large superfamily includes the catalytic domain (exonuclease/endonuclease/phosphatase or EEP domain) of a diverse set of proteins including the ExoIII family of apurinic/apyrimidinic (AP) endonucleases, inositol polyphosphate 5-phosphatases (INPP5), neutral sphingomyelinases (nSMases), deadenylases (such as the vertebrate circadian-clock regulated nocturnin), bacterial cytolethal distending toxin B (CdtB), deoxyribonuclease 1 (DNase1), the endonuclease domain of the non-LTR retrotransposon LINE-1, and related domains. These diverse enzymes share a common catalytic mechanism of cleaving phosphodiester bonds; their substrates range from nucleic acids to phospholipids and perhaps proteins.


The actual alignment was detected with superfamily member smart00476:

Pssm-ID: 469791  Cd Length: 276  Bit Score: 222.32  E-value: 1.20e-72
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 569010624    27 LLILLVGGTEAFRICAFNAHRLTLAKLTKESVMDTLVQILARCDIMVLQEVVDSSQNTVPFLLQKLK--SSRSYSFLNSS 104
Cdd:smart00476   7 LFLLLLHGAASLRICAFNIQSFGDSKMSNATLMSIIVKILSRYDIALVQEVRDSDLSAVPKLMDQLNsdSPNTYSYVSSE 86

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 569010624   105 LLGRSTYKEKYVYIYRSDKTQVLNFYQYNDT----DDIFAREPFVAHFTLPSKTLPSVVLVPLHTTPKDVEKELNALYDV 180
Cdd:smart00476  87 PLGRNSYKEQYLFLYRSDLVSVLDSYLYDDGcecgNDVFSREPFVVKFSSPSTAVKEFVIVPLHTTPEAAVAEIDALYDV 166

                          170
                   ....*....|.
gi 569010624   181 FLDVYQRWQNE 191
Cdd:smart00476 167 YLDVRQKWGTE 177
 
Name Accession Description Interval E-value
DNaseIc smart00476
deoxyribonuclease I; Deoxyribonuclease I catalyzes the endonucleolytic cleavage of ...
 27-191 1.20e-72

deoxyribonuclease I; Deoxyribonuclease I catalyzes the endonucleolytic cleavage of double-stranded DNA. The enzyme is secreted outside the cell and also involved in apoptosis in the nucleus.


Pssm-ID: 128752  Cd Length: 276  Bit Score: 222.32  E-value: 1.20e-72
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 569010624    27 LLILLVGGTEAFRICAFNAHRLTLAKLTKESVMDTLVQILARCDIMVLQEVVDSSQNTVPFLLQKLK--SSRSYSFLNSS 104
Cdd:smart00476   7 LFLLLLHGAASLRICAFNIQSFGDSKMSNATLMSIIVKILSRYDIALVQEVRDSDLSAVPKLMDQLNsdSPNTYSYVSSE 86

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 569010624   105 LLGRSTYKEKYVYIYRSDKTQVLNFYQYNDT----DDIFAREPFVAHFTLPSKTLPSVVLVPLHTTPKDVEKELNALYDV 180
Cdd:smart00476  87 PLGRNSYKEQYLFLYRSDLVSVLDSYLYDDGcecgNDVFSREPFVVKFSSPSTAVKEFVIVPLHTTPEAAVAEIDALYDV 166

                          170
                   ....*....|.
gi 569010624   181 FLDVYQRWQNE 191
Cdd:smart00476 167 YLDVRQKWGTE 177
DNase1 cd10282
Deoxyribonuclease 1; Deoxyribonuclease 1 (DNase1, EC 3.1.21.1), also known as DNase I, is a ...
 39-191 6.74e-72

Deoxyribonuclease 1; Deoxyribonuclease 1 (DNase1, EC 3.1.21.1), also known as DNase I, is a Ca2+, Mg2+/Mn2+-dependent secretory endonuclease, first isolated from bovine pancreas extracts. It cleaves DNA preferentially at phosphodiester linkages next to a pyrimidine nucleotide, producing 5'-phosphate terminated polynucleotides with a free hydroxyl group on position 3'. It generally produces tetranucleotides. DNase1 substrates include single-stranded DNA, double-stranded DNA, and chromatin. This enzyme may be responsible for apoptotic DNA fragmentation. Other deoxyribonucleases in this subfamily include human DNL1L (human DNase I lysosomal-like, also known as DNASE1L1, Xib, and DNase X ), human DNASE1L2 (also known as DNAS1L2), and DNASE1L3 (also known as DNAS1L3, nhDNase, LS-DNase, DNase Y, and DNase gamma) . DNASE1L3 is implicated in apoptotic DNA fragmentation. DNase I is also a cytoskeletal protein which binds actin. A recombinant form of human DNase1 is used as a mucoactive therapy in patients with cystic fibrosis; it hydrolyzes the extracellular DNA in sputum and reduces its viscosity. Mutations in the gene encoding DNase1 have been associated with Systemic Lupus Erythematosus, a multifactorial autoimmune disease. This subfamily belongs to the large EEP (exonuclease/endonuclease/phosphatase) superfamily that contains functionally diverse enzymes that share a common catalytic mechanism of cleaving phosphodiester bonds.


Pssm-ID: 197337 [Multi-domain]  Cd Length: 256  Bit Score: 219.42  E-value: 6.74e-72
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 569010624  39 RICAFNAHRLTLAKLTKESVMDTLVQILARCDIMVLQEVVDSSQNTVPFLLQKL--KSSRSYSFLNSSLLGRSTYKEKYV 116
Cdd:cd10282    1 RIAAFNIQVFGESKMSKPEVMDVLVKILSRYDIVLIQEIRDSSGTAIPELLDELnsASSNTYSYVVSERLGRSSYKEQYA 80

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 569010624 117 YIYRSDKTQVLNFYQYNDTD---DIFAREPFVAHFTLPSKTLPSVVLVPLHTTPKDVEKELNALYDVFLDVYQRWQNE 191
Cdd:cd10282   81 FIYRSDKVSVLESYQYDDGDegtDVFSREPFVVRFSSPSTAVKDFVLVPIHTSPDDAVAEIDALYDVYDDVKQRWRED 158
Exo_endo_phos pfam03372
Endonuclease/Exonuclease/phosphatase family; This large family of proteins includes magnesium ...
 43-193 1.60e-04

Endonuclease/Exonuclease/phosphatase family; This large family of proteins includes magnesium dependent endonucleases and a large number of phosphatases involved in intracellular signalling. This family includes: AP endonuclease proteins EC:4.2.99.18, DNase I proteins EC:3.1.21.1, Synaptojanin an inositol-1,4,5-trisphosphate phosphatase EC:3.1.3.56, Sphingomyelinase EC:3.1.4.12 and Nocturnin.


Pssm-ID: 460902 [Multi-domain]  Cd Length: 183  Bit Score: 41.06  E-value: 1.60e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 569010624   43 FNAHRLTLAKLTKESVMDTLVQILARC--DIMVLQEVVDSSQNTVPFLLQKLkssrsYSFLNSSLLGRSTYKEKYVYIYR 120
Cdd:pfam03372   3 WNVNGGNADAAGDDRKLDALAALIRAYdpDVVALQETDDDDASRLLLALLAY-----GGFLSYGGPGGGGGGGGVAILSR 77

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 569010624  121 SDKTQVLNFYQYNDTDDIFAREPFVAHFTLPsktLPSVVLVPLHTTPKDVEKELNALYDVFLDVYQRWQNEAL 193
Cdd:pfam03372  78 YPLSSVILVDLGEFGDPALRGAIAPFAGVLV---VPLVLTLAPHASPRLARDEQRADLLLLLLALLAPRSEPV 147
 
Name Accession Description Interval E-value
DNaseIc smart00476
deoxyribonuclease I; Deoxyribonuclease I catalyzes the endonucleolytic cleavage of ...
 27-191 1.20e-72

deoxyribonuclease I; Deoxyribonuclease I catalyzes the endonucleolytic cleavage of double-stranded DNA. The enzyme is secreted outside the cell and also involved in apoptosis in the nucleus.


Pssm-ID: 128752  Cd Length: 276  Bit Score: 222.32  E-value: 1.20e-72
                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 569010624    27 LLILLVGGTEAFRICAFNAHRLTLAKLTKESVMDTLVQILARCDIMVLQEVVDSSQNTVPFLLQKLK--SSRSYSFLNSS 104
Cdd:smart00476   7 LFLLLLHGAASLRICAFNIQSFGDSKMSNATLMSIIVKILSRYDIALVQEVRDSDLSAVPKLMDQLNsdSPNTYSYVSSE 86

                           90       100       110       120       130       140       150       160
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 569010624   105 LLGRSTYKEKYVYIYRSDKTQVLNFYQYNDT----DDIFAREPFVAHFTLPSKTLPSVVLVPLHTTPKDVEKELNALYDV 180
Cdd:smart00476  87 PLGRNSYKEQYLFLYRSDLVSVLDSYLYDDGcecgNDVFSREPFVVKFSSPSTAVKEFVIVPLHTTPEAAVAEIDALYDV 166

                          170
                   ....*....|.
gi 569010624   181 FLDVYQRWQNE 191
Cdd:smart00476 167 YLDVRQKWGTE 177
DNase1 cd10282
Deoxyribonuclease 1; Deoxyribonuclease 1 (DNase1, EC 3.1.21.1), also known as DNase I, is a ...
 39-191 6.74e-72

Deoxyribonuclease 1; Deoxyribonuclease 1 (DNase1, EC 3.1.21.1), also known as DNase I, is a Ca2+, Mg2+/Mn2+-dependent secretory endonuclease, first isolated from bovine pancreas extracts. It cleaves DNA preferentially at phosphodiester linkages next to a pyrimidine nucleotide, producing 5'-phosphate terminated polynucleotides with a free hydroxyl group on position 3'. It generally produces tetranucleotides. DNase1 substrates include single-stranded DNA, double-stranded DNA, and chromatin. This enzyme may be responsible for apoptotic DNA fragmentation. Other deoxyribonucleases in this subfamily include human DNL1L (human DNase I lysosomal-like, also known as DNASE1L1, Xib, and DNase X ), human DNASE1L2 (also known as DNAS1L2), and DNASE1L3 (also known as DNAS1L3, nhDNase, LS-DNase, DNase Y, and DNase gamma) . DNASE1L3 is implicated in apoptotic DNA fragmentation. DNase I is also a cytoskeletal protein which binds actin. A recombinant form of human DNase1 is used as a mucoactive therapy in patients with cystic fibrosis; it hydrolyzes the extracellular DNA in sputum and reduces its viscosity. Mutations in the gene encoding DNase1 have been associated with Systemic Lupus Erythematosus, a multifactorial autoimmune disease. This subfamily belongs to the large EEP (exonuclease/endonuclease/phosphatase) superfamily that contains functionally diverse enzymes that share a common catalytic mechanism of cleaving phosphodiester bonds.


Pssm-ID: 197337 [Multi-domain]  Cd Length: 256  Bit Score: 219.42  E-value: 6.74e-72
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 569010624  39 RICAFNAHRLTLAKLTKESVMDTLVQILARCDIMVLQEVVDSSQNTVPFLLQKL--KSSRSYSFLNSSLLGRSTYKEKYV 116
Cdd:cd10282    1 RIAAFNIQVFGESKMSKPEVMDVLVKILSRYDIVLIQEIRDSSGTAIPELLDELnsASSNTYSYVVSERLGRSSYKEQYA 80

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 569010624 117 YIYRSDKTQVLNFYQYNDTD---DIFAREPFVAHFTLPSKTLPSVVLVPLHTTPKDVEKELNALYDVFLDVYQRWQNE 191
Cdd:cd10282   81 FIYRSDKVSVLESYQYDDGDegtDVFSREPFVVRFSSPSTAVKDFVLVPIHTSPDDAVAEIDALYDVYDDVKQRWRED 158
DNase1-like cd09075
Deoxyribonuclease 1 and related proteins; This family includes Deoxyribonuclease 1 (DNase1, EC ...
 39-193 8.24e-28

Deoxyribonuclease 1 and related proteins; This family includes Deoxyribonuclease 1 (DNase1, EC 3.1.21.1) and related proteins. DNase1, also known as DNase I, is a Ca2+, Mg2+/Mn2+-dependent secretory endonuclease, first isolated from bovine pancreas extracts. It cleaves DNA preferentially at phosphodiester linkages next to a pyrimidine nucleotide, producing 5'-phosphate terminated polynucleotides with a free hydroxyl group on position 3'. It generally produces tetranucleotides. DNase1 substrates include single-stranded DNA, double-stranded DNA, and chromatin. This enzyme may be responsible for apoptotic DNA fragmentation. Other deoxyribonucleases in this subfamily include human DNL1L (human DNase I lysosomal-like, also known as DNASE1L1, Xib and DNase X ), human DNASE1L2 (also known as DNAS1L2), and DNASE1L3 (also known as DNAS1L3, nhDNase, LS-DNase, DNase Y, and DNase gamma). DNASE1L3 is also implicated in apoptotic DNA fragmentation. DNase1 is also a cytoskeletal protein which binds actin. A recombinant form of human DNase1 is used as a mucoactive therapy in patients with cystic fibrosis; it hydrolyzes the extracellular DNA in sputum and reduces its viscosity. Mutations in the gene encoding DNase1 have been associated with Systemic Lupus Erythematosus, a multifactorial autoimmune disease. This family also includes a subfamily of mostly uncharacterized proteins, which includes Mycoplasma pulmonis MnuA, a membrane-associated nuclease. The in vivo role of MnuA is as yet undetermined. This family belongs to the large EEP (exonuclease/endonuclease/phosphatase) superfamily that contains functionally diverse enzymes that share a common catalytic mechanism of cleaving phosphodiester bonds.


Pssm-ID: 197309 [Multi-domain]  Cd Length: 258  Bit Score: 106.33  E-value: 8.24e-28
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 569010624  39 RICAFNAHRLTLAKLTKESVMDTLVQILARCDIMVLQEVVDSSQNTVPFLLQKLK--SSRSYSFLNSSLLGRSTYKEKYV 116
Cdd:cd09075    1 KIAAFNIRTFGETKMSNATLASYIVRIVRRYDIVLIQEVRDSHLVAVGKLLDYLNqdDPNTYHYVVSEPLGRNSYKERYL 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 569010624 117 YIYRSDKTQVLNFYQYNDT-----DDIFAREPFVAHFTLPSKTLPSVVLVPLHTTPKDVEKELNALYDVFLDVYQRWQNE 191
Cdd:cd09075   81 FLFRPNKVSVLDTYQYDDGckscgNDSFSREPAVVKFSSHSTKVKEFAIVALHSAPSDAVAEINSLYDVYLDVQQKWHLN 160


                 ..
gi 569010624 192 AL 193
Cdd:cd09075  161 DV 162
MnuA_DNase1-like cd10283
Mycoplasma pulmonis MnuA nuclease-like; This subfamily includes Mycoplasma pulmonis MnuA, a ...
 38-173 1.56e-10

Mycoplasma pulmonis MnuA nuclease-like; This subfamily includes Mycoplasma pulmonis MnuA, a membrane-associated nuclease related to Deoxyribonuclease 1 (DNase1 or DNase I, EC 3.1.21.1). The in vivo role of MnuA is as yet undetermined. This subfamily belongs to the large EEP (exonuclease/endonuclease/phosphatase) superfamily that contains functionally diverse enzymes that share a common catalytic mechanism of cleaving phosphodiester bonds.


Pssm-ID: 197338 [Multi-domain]  Cd Length: 266  Bit Score: 59.33  E-value: 1.56e-10
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 569010624  38 FRICAFNAHRLTLAKlTKESVmDTLVQILAR--CDIMVLQEVVDSSQ--NTVPFLLQKLKSSRS-YSFLNSS-LLGRSTY 111
Cdd:cd10283    1 LRIASWNILNFGNSK-GKEKN-PAIAEIISAfdLDLIALQEVMDNGGglDALAKLVNELNKPGGtWKYIVSDkTGGSSGD 78

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*
gi 569010624 112 KEKYVYIYRSDKTQ-VLNFYQYNDT-DDIFAREPFVAHF-TLPSKTlpSVVLVPLHTTPKDVEKE 173
Cdd:cd10283   79 KERYAFLYKSSKVRkVGKAVLEKDSnTDGFARPPYAAKFkSGGTGF--DFTLVNVHLKSGGSSKS 141
Exo_endo_phos pfam03372
Endonuclease/Exonuclease/phosphatase family; This large family of proteins includes magnesium ...
 43-193 1.60e-04

Endonuclease/Exonuclease/phosphatase family; This large family of proteins includes magnesium dependent endonucleases and a large number of phosphatases involved in intracellular signalling. This family includes: AP endonuclease proteins EC:4.2.99.18, DNase I proteins EC:3.1.21.1, Synaptojanin an inositol-1,4,5-trisphosphate phosphatase EC:3.1.3.56, Sphingomyelinase EC:3.1.4.12 and Nocturnin.


Pssm-ID: 460902 [Multi-domain]  Cd Length: 183  Bit Score: 41.06  E-value: 1.60e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 569010624   43 FNAHRLTLAKLTKESVMDTLVQILARC--DIMVLQEVVDSSQNTVPFLLQKLkssrsYSFLNSSLLGRSTYKEKYVYIYR 120
Cdd:pfam03372   3 WNVNGGNADAAGDDRKLDALAALIRAYdpDVVALQETDDDDASRLLLALLAY-----GGFLSYGGPGGGGGGGGVAILSR 77

                          90       100       110       120       130       140       150
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 569010624  121 SDKTQVLNFYQYNDTDDIFAREPFVAHFTLPsktLPSVVLVPLHTTPKDVEKELNALYDVFLDVYQRWQNEAL 193
Cdd:pfam03372  78 YPLSSVILVDLGEFGDPALRGAIAPFAGVLV---VPLVLTLAPHASPRLARDEQRADLLLLLLALLAPRSEPV 147
EEP cd08372
Exonuclease-Endonuclease-Phosphatase (EEP) domain superfamily; This large superfamily includes ...
 40-191 3.33e-03

Exonuclease-Endonuclease-Phosphatase (EEP) domain superfamily; This large superfamily includes the catalytic domain (exonuclease/endonuclease/phosphatase or EEP domain) of a diverse set of proteins including the ExoIII family of apurinic/apyrimidinic (AP) endonucleases, inositol polyphosphate 5-phosphatases (INPP5), neutral sphingomyelinases (nSMases), deadenylases (such as the vertebrate circadian-clock regulated nocturnin), bacterial cytolethal distending toxin B (CdtB), deoxyribonuclease 1 (DNase1), the endonuclease domain of the non-LTR retrotransposon LINE-1, and related domains. These diverse enzymes share a common catalytic mechanism of cleaving phosphodiester bonds; their substrates range from nucleic acids to phospholipids and perhaps proteins.


Pssm-ID: 197306 [Multi-domain]  Cd Length: 241  Bit Score: 37.46  E-value: 3.33e-03
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 569010624  40 ICAFNAHRLTLAKLTKEsvmdtLVQILARC--DIMVLQEVVDsSQNTVPFLLQKLKssrSYSFLNSSLLGRSTYKEKYVY 117
Cdd:cd08372    1 VASYNVNGLNAATRASG-----IARWVRELdpDIVCLQEVKD-SQYSAVALNQLLP---EGYHQYQSGPSRKEGYEGVAI 71

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 569010624 118 IYRSDKTQVLNFYQYND-TDDIFAREPFVAHFTLPSKtlpSVVLVPLH-----TTPKDVEKELNALYDVFLDVyQRWQNE 191
Cdd:cd08372   72 LSKTPKFKIVEKHQYKFgEGDSGERRAVVVKFDVHDK---ELCVVNAHlqaggTRADVRDAQLKEVLEFLKRL-RQPNSA 147
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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