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Conserved domains on  [gi|755537116|ref|XP_011246975|]
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aspartoacylase isoform X4 [Mus musculus]

Protein Classification

M14 family aspartoacylase( domain architecture ID 10161027)

M14 family aspartoacylase (ASPA) cleaves N-acetyl L-aspartic acid (NAA) into aspartate and acetate

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
M14_ASPA cd06909
Peptidase M14 Aspartoacylase (ASPA) subfamily; Aspartoacylase (ASPA) belongs to the ...
 11-175 1.60e-83

Peptidase M14 Aspartoacylase (ASPA) subfamily; Aspartoacylase (ASPA) belongs to the Succinylglutamate desuccinylase/aspartoacylase subfamily of the M14 family of metallocarboxypeptidases. ASPA (also known as aminoacylase 2; EC:3.5.1.15) cleaves N-acetyl L-aspartic acid (NAA) into aspartate and acetate. NAA is abundant in the brain, and hydrolysis of NAA by ASPA may help maintain white matter. ASPA is an NAA scavenger in other tissues. Mutations in the gene encoding ASPA cause Canavan disease (CD), a fatal progressive neurodegenerative disorder involving dysmyelination and spongiform degeneration of white matter in children. This enzyme binds zinc which is necessary for activity. Measurement of elevated NAA levels in urine is used in the diagnosis of CD.


:

Pssm-ID: 349480  Cd Length: 190  Bit Score: 244.81  E-value: 1.60e-83
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755537116  11 KKIAIFGGTHGNELTGVFLVTHWLRNGTEVHRAGLDVKPFITNPRAVEKCTRYIDCDLNRVFDLENLSKEmSEDLPYEVR 90
Cdd:cd06909    1 KRVAIVGGTHGNELTGVYLVKHWLKNPELIERKSFEVHPLLANPRAVEQCRRYIDTDLNRCFSLENLSSA-PSSLPYEVR 79

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755537116  91 RAQEINHLFGPKNsDDAYDLVFDLHNTTSNMGCTLILEDSRnDFLIQMFHYIKTCMapLPCSVYLIEHPSLKYATTRSIA 170
Cdd:cd06909   80 RAREINQILGPKG-NPACDFIIDLHNTTSNMGITLILSSSD-DFTLKLAAYLQQRL--PPVRVLLHESPSKESPFLRSVA 155


                 ....*
gi 755537116 171 KYPVG 175
Cdd:cd06909  156 KHGFT 160
 
Name Accession Description Interval E-value
M14_ASPA cd06909
Peptidase M14 Aspartoacylase (ASPA) subfamily; Aspartoacylase (ASPA) belongs to the ...
 11-175 1.60e-83

Peptidase M14 Aspartoacylase (ASPA) subfamily; Aspartoacylase (ASPA) belongs to the Succinylglutamate desuccinylase/aspartoacylase subfamily of the M14 family of metallocarboxypeptidases. ASPA (also known as aminoacylase 2; EC:3.5.1.15) cleaves N-acetyl L-aspartic acid (NAA) into aspartate and acetate. NAA is abundant in the brain, and hydrolysis of NAA by ASPA may help maintain white matter. ASPA is an NAA scavenger in other tissues. Mutations in the gene encoding ASPA cause Canavan disease (CD), a fatal progressive neurodegenerative disorder involving dysmyelination and spongiform degeneration of white matter in children. This enzyme binds zinc which is necessary for activity. Measurement of elevated NAA levels in urine is used in the diagnosis of CD.


Pssm-ID: 349480  Cd Length: 190  Bit Score: 244.81  E-value: 1.60e-83
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755537116  11 KKIAIFGGTHGNELTGVFLVTHWLRNGTEVHRAGLDVKPFITNPRAVEKCTRYIDCDLNRVFDLENLSKEmSEDLPYEVR 90
Cdd:cd06909    1 KRVAIVGGTHGNELTGVYLVKHWLKNPELIERKSFEVHPLLANPRAVEQCRRYIDTDLNRCFSLENLSSA-PSSLPYEVR 79

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755537116  91 RAQEINHLFGPKNsDDAYDLVFDLHNTTSNMGCTLILEDSRnDFLIQMFHYIKTCMapLPCSVYLIEHPSLKYATTRSIA 170
Cdd:cd06909   80 RAREINQILGPKG-NPACDFIIDLHNTTSNMGITLILSSSD-DFTLKLAAYLQQRL--PPVRVLLHESPSKESPFLRSVA 155


                 ....*
gi 755537116 171 KYPVG 175
Cdd:cd06909  156 KHGFT 160
PRK02259 PRK02259
aspartoacylase; Provisional
 10-159 1.48e-54

aspartoacylase; Provisional


Pssm-ID: 235019  Cd Length: 288  Bit Score: 174.29  E-value: 1.48e-54
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755537116  10 IKKIAIFGGTHGNELTGVFLVTHWLRNGTEVHRAGLDVKPFITNPRAVEKCTRYIDCDLNRVFDLENLskEMSEDLPYEV 89
Cdd:PRK02259   2 INRVAIVGGTHGNEITGIYLVKKWQQQPNLINRKGLEVQTVIGNPEAIEAGRRYIDRDLNRSFRLDLL--QNPDLSGYEQ 79

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755537116  90 RRAQEINHLFGPKNSDDaYDLVFDLHNTTSNMGCTLILEDsRNDFLIQMFHYIKTCMaPLPcsVYLIEHP 159
Cdd:PRK02259  80 LRAKELVQQLGPKGNSP-CDFIIDLHSTTANMGLSLILYG-RRPFDLALAAYLQSRL-PLP--IYLHEKD 144
AstE_AspA pfam04952
Succinylglutamate desuccinylase / Aspartoacylase family; This family includes ...
 9-175 4.21e-52

Succinylglutamate desuccinylase / Aspartoacylase family; This family includes Succinylglutamate desuccinylase EC:3.1.-.- that catalyzes the fifth and last step in arginine catabolism by the arginine succinyltransferase pathway. The family also include aspartoacylase EC:3.5.1.15 which cleaves acylaspartate into a fatty acid and aspartate. Mutations in Swiss:P45381 lead to Canavan disease. This family is probably structurally related to pfam00246 (Bateman A pers. obs.).


Pssm-ID: 428216 [Multi-domain]  Cd Length: 289  Bit Score: 168.30  E-value: 4.21e-52
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755537116    9 PIKKIAIFGGTHGNELTGVFLVTHWLRNGTEVHRAGLDVKPFITNPRAVEKCTRYIDCDLNRVFDLENLSKemSEDLPYE 88
Cdd:pfam04952   1 PGPTLLLSAGIHGNETNGVELLRRLLRQLDPGDIAGERTLVPLANPPAFRAGSRYIPRDLNRSFPGRALGA--SSDEPYR 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755537116   89 VRRAQEINHLFGPKNSdDAYDLVFDLHNTTSNMGCTLILEDSRNDFLIQMFHYIKTCMAPLPCSVYLIEHPSLKYATTRS 168
Cdd:pfam04952  79 ATRAERLADLFFPALL-PRADIVLDLHTGTRGMGHLLFALAPIRDDPLHLLALLRAFGAPAVLKLHSKPSAGFSAFSAEE 157


                  ....*..
gi 755537116  169 IAKYPVG 175
Cdd:pfam04952 158 LGAPGFT 164
AstE COG2988
Succinylglutamate desuccinylase [Amino acid transport and metabolism];
10-146 9.76e-35

Succinylglutamate desuccinylase [Amino acid transport and metabolism];


Pssm-ID: 442227  Cd Length: 305  Bit Score: 123.80  E-value: 9.76e-35
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755537116  10 IKKIAIFGGTHGNELTGVFLVTHWLRNGTEVHRA-GLDVKPFITNPRAVEKCTRYIDCDLNRVFDLENLSKEMSedlpYE 88
Cdd:COG2988   24 IKAVVISGGIHGNETAPIELLDKLLQDLLLGERPlSFRLLLILGNPAAMRAGRRYLDEDLNRLFGGRHLQNPES----YE 99

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 755537116  89 VRRAQEINHLFGPK-NSDDAYDLVFDLHNTTSNMGCTLI--LEDSRNDFLIQMFHYIKTCM 146
Cdd:COG2988  100 AARAKELEQAVGPFfAAGGRVRLHIDLHTAIRNSGHERFavYPFRGRPFDLALLAYLAAAG 160
 
Name Accession Description Interval E-value
M14_ASPA cd06909
Peptidase M14 Aspartoacylase (ASPA) subfamily; Aspartoacylase (ASPA) belongs to the ...
 11-175 1.60e-83

Peptidase M14 Aspartoacylase (ASPA) subfamily; Aspartoacylase (ASPA) belongs to the Succinylglutamate desuccinylase/aspartoacylase subfamily of the M14 family of metallocarboxypeptidases. ASPA (also known as aminoacylase 2; EC:3.5.1.15) cleaves N-acetyl L-aspartic acid (NAA) into aspartate and acetate. NAA is abundant in the brain, and hydrolysis of NAA by ASPA may help maintain white matter. ASPA is an NAA scavenger in other tissues. Mutations in the gene encoding ASPA cause Canavan disease (CD), a fatal progressive neurodegenerative disorder involving dysmyelination and spongiform degeneration of white matter in children. This enzyme binds zinc which is necessary for activity. Measurement of elevated NAA levels in urine is used in the diagnosis of CD.


Pssm-ID: 349480  Cd Length: 190  Bit Score: 244.81  E-value: 1.60e-83
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755537116  11 KKIAIFGGTHGNELTGVFLVTHWLRNGTEVHRAGLDVKPFITNPRAVEKCTRYIDCDLNRVFDLENLSKEmSEDLPYEVR 90
Cdd:cd06909    1 KRVAIVGGTHGNELTGVYLVKHWLKNPELIERKSFEVHPLLANPRAVEQCRRYIDTDLNRCFSLENLSSA-PSSLPYEVR 79

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755537116  91 RAQEINHLFGPKNsDDAYDLVFDLHNTTSNMGCTLILEDSRnDFLIQMFHYIKTCMapLPCSVYLIEHPSLKYATTRSIA 170
Cdd:cd06909   80 RAREINQILGPKG-NPACDFIIDLHNTTSNMGITLILSSSD-DFTLKLAAYLQQRL--PPVRVLLHESPSKESPFLRSVA 155


                 ....*
gi 755537116 171 KYPVG 175
Cdd:cd06909  156 KHGFT 160
PRK02259 PRK02259
aspartoacylase; Provisional
 10-159 1.48e-54

aspartoacylase; Provisional


Pssm-ID: 235019  Cd Length: 288  Bit Score: 174.29  E-value: 1.48e-54
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755537116  10 IKKIAIFGGTHGNELTGVFLVTHWLRNGTEVHRAGLDVKPFITNPRAVEKCTRYIDCDLNRVFDLENLskEMSEDLPYEV 89
Cdd:PRK02259   2 INRVAIVGGTHGNEITGIYLVKKWQQQPNLINRKGLEVQTVIGNPEAIEAGRRYIDRDLNRSFRLDLL--QNPDLSGYEQ 79

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755537116  90 RRAQEINHLFGPKNSDDaYDLVFDLHNTTSNMGCTLILEDsRNDFLIQMFHYIKTCMaPLPcsVYLIEHP 159
Cdd:PRK02259  80 LRAKELVQQLGPKGNSP-CDFIIDLHSTTANMGLSLILYG-RRPFDLALAAYLQSRL-PLP--IYLHEKD 144
AstE_AspA pfam04952
Succinylglutamate desuccinylase / Aspartoacylase family; This family includes ...
 9-175 4.21e-52

Succinylglutamate desuccinylase / Aspartoacylase family; This family includes Succinylglutamate desuccinylase EC:3.1.-.- that catalyzes the fifth and last step in arginine catabolism by the arginine succinyltransferase pathway. The family also include aspartoacylase EC:3.5.1.15 which cleaves acylaspartate into a fatty acid and aspartate. Mutations in Swiss:P45381 lead to Canavan disease. This family is probably structurally related to pfam00246 (Bateman A pers. obs.).


Pssm-ID: 428216 [Multi-domain]  Cd Length: 289  Bit Score: 168.30  E-value: 4.21e-52
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755537116    9 PIKKIAIFGGTHGNELTGVFLVTHWLRNGTEVHRAGLDVKPFITNPRAVEKCTRYIDCDLNRVFDLENLSKemSEDLPYE 88
Cdd:pfam04952   1 PGPTLLLSAGIHGNETNGVELLRRLLRQLDPGDIAGERTLVPLANPPAFRAGSRYIPRDLNRSFPGRALGA--SSDEPYR 78

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755537116   89 VRRAQEINHLFGPKNSdDAYDLVFDLHNTTSNMGCTLILEDSRNDFLIQMFHYIKTCMAPLPCSVYLIEHPSLKYATTRS 168
Cdd:pfam04952  79 ATRAERLADLFFPALL-PRADIVLDLHTGTRGMGHLLFALAPIRDDPLHLLALLRAFGAPAVLKLHSKPSAGFSAFSAEE 157


                  ....*..
gi 755537116  169 IAKYPVG 175
Cdd:pfam04952 158 LGAPGFT 164
AstE COG2988
Succinylglutamate desuccinylase [Amino acid transport and metabolism];
10-146 9.76e-35

Succinylglutamate desuccinylase [Amino acid transport and metabolism];


Pssm-ID: 442227  Cd Length: 305  Bit Score: 123.80  E-value: 9.76e-35
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755537116  10 IKKIAIFGGTHGNELTGVFLVTHWLRNGTEVHRA-GLDVKPFITNPRAVEKCTRYIDCDLNRVFDLENLSKEMSedlpYE 88
Cdd:COG2988   24 IKAVVISGGIHGNETAPIELLDKLLQDLLLGERPlSFRLLLILGNPAAMRAGRRYLDEDLNRLFGGRHLQNPES----YE 99

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|.
gi 755537116  89 VRRAQEINHLFGPK-NSDDAYDLVFDLHNTTSNMGCTLI--LEDSRNDFLIQMFHYIKTCM 146
Cdd:COG2988  100 AARAKELEQAVGPFfAAGGRVRLHIDLHTAIRNSGHERFavYPFRGRPFDLALLAYLAAAG 160
M14_ASTE_ASPA_like cd06230
Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA) subfamily; The ...
 13-175 4.37e-21

Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA) subfamily; The Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA) subfamily belongs to the M14 family of metallocarboxypeptidases (MCPs), and includes ASTE, which catalyzes the fifth and last step in arginine catabolism by the arginine succinyltransferase pathway, and aspartoacylase (ASPA, also known as aminoacylase 2, and ACY-2; EC:3.5.1.15) which cleaves N-acetyl L-aspartic acid (NAA) into aspartate and acetate. NAA is abundant in the brain, and hydrolysis of NAA by ASPA may help maintain white matter. ASPA is an NAA scavenger in other tissues. Mutations in the gene encoding ASPA cause Canavan disease (CD), a fatal progressive neurodegenerative disorder involving dysmyelination and spongiform degeneration of white matter in children. This enzyme binds zinc which is necessary for activity. Measurement of elevated NAA levels in urine is used in the diagnosis of CD.


Pssm-ID: 349449 [Multi-domain]  Cd Length: 177  Bit Score: 85.06  E-value: 4.37e-21
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755537116  13 IAIFGGTHGNELTGVFLVTHWLRNGTEVHRAG-LDVKPfITNPRAVEKCTRYID---CDLNRVFDLenlskemSEDLPYE 88
Cdd:cd06230    1 LLILAGVHGDEYEGVEAIRRLLAELDPSELKGtVVLVP-VANPPAFEAGTRYTPldgLDLNRIFPG-------DPDGSPT 72

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755537116  89 VRRAQEINHLFGPKnsddaYDLVFDLHNTTSNM-GCTLILEDSR--NDFLIQMFHYIKtcmaplPCSVYLIEHP--SLKY 163
Cdd:cd06230   73 ERLAHELTELILKH-----ADALIDLHSGGTGRlVPYAILDYDSdaREKSRELARAFG------GTPVIWGGDPpgGTPV 141

                        170
                 ....*....|..
gi 755537116 164 ATTRSIAKYPVG 175
Cdd:cd06230  142 AAARSAGIPAIT 153
M14_ASTE_ASPA_like cd18430
Succinylglutamate desuccinylase/aspartoacylase; uncharacterized; A functionally ...
 13-133 2.30e-11

Succinylglutamate desuccinylase/aspartoacylase; uncharacterized; A functionally uncharacterized subgroup of the Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA) subfamily which is part of the M14 family of metallocarboxypeptidases. ASTE catalyzes the fifth and last step in arginine catabolism by the arginine succinyltransferase pathway, and aspartoacylase (ASPA, also known as aminoacylase 2, and ACY-2; EC:3.5.1.15) cleaves N-acetyl L-aspartic acid (NAA) into aspartate and acetate. NAA is abundant in the brain, and hydrolysis of NAA by ASPA may help maintain white matter. ASPA is an NAA scavenger in other tissues. Mutations in the gene encoding ASPA cause Canavan disease (CD), a fatal progressive neurodegenerative disorder involving dysmyelination and spongiform degeneration of white matter in children. This enzyme binds zinc which is necessary for activity. Measurement of elevated NAA levels in urine is used in the diagnosis of CD.


Pssm-ID: 349486 [Multi-domain]  Cd Length: 168  Bit Score: 59.38  E-value: 2.30e-11
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755537116  13 IAIFGGTHGNELTGVF--------LVTHWLRNGTevhragldVKPFITNPRAVEKCTRYIDCDLNRVFDLENLSKEmsed 84
Cdd:cd18430    1 LAVLGAVHGNETCGTRaverllaeLPSGALQKGP--------VTLVPANERAYAEGVRFCEEDLNRVFPGDPDPDT---- 68

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*....
gi 755537116  85 lpYEVRRAQEINHLFgpknsdDAYDLVFDLHNTTSNmGCTLILEDSRND 133
Cdd:cd18430   69 --YERRLANRLCPEL------EGHDVVLDLHSTHSG-GQPFAILDYGDK 108
M14_ASTE_ASPA-like cd06910
Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA)-like; ...
 15-118 2.15e-08

Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA)-like; uncharacterized subgroup; A functionally uncharacterized subgroup of the Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA) subfamily which is part of the M14 family of metallocarboxypeptidases. ASTE catalyzes the fifth and last step in arginine catabolism by the arginine succinyltransferase pathway, and aspartoacylase (ASPA, also known as aminoacylase 2, and ACY-2; EC:3.5.1.15) cleaves N-acetyl L-aspartic acid (NAA) into aspartate and acetate. NAA is abundant in the brain, and hydrolysis of NAA by ASPA may help maintain white matter. ASPA is an NAA scavenger in other tissues. Mutations in the gene encoding ASPA cause Canavan disease (CD), a fatal progressive neurodegenerative disorder involving dysmyelination and spongiform degeneration of white matter in children. This enzyme binds zinc which is necessary for activity. Measurement of elevated NAA levels in urine is used in the diagnosis of CD.


Pssm-ID: 349481  Cd Length: 208  Bit Score: 51.58  E-value: 2.15e-08
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755537116  15 IFGGTHGNELTGVFLVTHWLRNGTEVHRAGLDVKpfITNPRAVEK-------CTRYIDCDLNRVFDLENLSkemSEDLPY 87
Cdd:cd06910   29 INALTHGNEICGAIALDWLLKNGVRPLRGRLTFC--FANVEAYERfdparptASRFVDEDLNRVWGPELLD---GPEQSI 103

                         90       100       110
                 ....*....|....*....|....*....|.
gi 755537116  88 EVRRAQEINHLFgpknsdDAYDLVFDLHNTT 118
Cdd:cd06910  104 ELRRARELRPVV------DTVDYLLDIHSMQ 128
M14_ASTE_ASPA-like cd06253
Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA)-like; ...
 11-121 1.77e-06

Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA)-like; uncharacterized subgroup; A functionally uncharacterized subgroup of the Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA) subfamily which is part of the M14 family of metallocarboxypeptidases. ASTE catalyzes the fifth and last step in arginine catabolism by the arginine succinyltransferase pathway, and aspartoacylase (ASPA, also known as aminoacylase 2, and ACY-2; EC:3.5.1.15) cleaves N-acetyl L-aspartic acid (NAA) into aspartate and acetate. NAA is abundant in the brain, and hydrolysis of NAA by ASPA may help maintain white matter. ASPA is an NAA scavenger in other tissues. Mutations in the gene encoding ASPA cause Canavan disease (CD), a fatal progressive neurodegenerative disorder involving dysmyelination and spongiform degeneration of white matter in children. This enzyme binds zinc which is necessary for activity. Measurement of elevated NAA levels in urine is used in the diagnosis of CD.


Pssm-ID: 349471  Cd Length: 211  Bit Score: 46.44  E-value: 1.77e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755537116  11 KKIAIFGGTHGNELTGVFL---VTHWLRN---GTEVHRAGLDVKPFItNPRAVEKCTRYI---DCDLNRVFDLENLSkEM 81
Cdd:cd06253   23 PRIAIVAGIHGDELNGLYVcsrLIRFLKEleeGGYKLKGKVLVIPAV-NPLGINSGTRFWpfdNLDMNRMFPGYNKG-ET 100

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|.
gi 755537116  82 SEdlpyevRRAQEI-NHLFGpknsddaYDLVFDLHNttSNM 121
Cdd:cd06253  101 TE------RIAAALfEDLKG-------ADYGIDLHS--SND 126
M14_REP34-like cd06231
Peptidase M14-like domain similar to rapid encystment phenotype 34 (REP34); This family ...
 11-118 1.84e-06

Peptidase M14-like domain similar to rapid encystment phenotype 34 (REP34); This family includes Francisella tularensis protein rapid encystment phenotype 34 (REP34) which is a zinc-containing monomeric protein demonstrating carboxypeptidase B-like activity. REP34 possesses a novel topology with its substrate binding pocket deviating from the canonical M14 peptidases with a possible catalytic role for a conserved tyrosine and distinct S1' recognition site. Thus, REP34, identified as an active carboxypeptidase and a potential key F. tularensis effector protein, may help elucidate a mechanistic understanding of F. tularensis infection of phagocytic cells. A functionally uncharacterized subgroup of the M14 family of metallocarboxypeptidases (MCPs). The M14 family are zinc-binding carboxypeptidases (CPs) which hydrolyze single, C-terminal amino acids from polypeptide chains, and have a recognition site for the free C-terminal carboxyl group, which is a key determinant of specificity. Two major subfamilies of the M14 family, defined based on sequence and structural homology, are the A/B and N/E subfamilies. Enzymes belonging to the A/B subfamily are normally synthesized as inactive precursors containing preceding signal peptide, followed by an N-terminal pro-region linked to the enzyme; these proenzymes are called procarboxypeptidases. The A/B enzymes can be further divided based on their substrate specificity; Carboxypeptidase A-like (CPA-like) enzymes favor hydrophobic residues while carboxypeptidase B-like (CPB-like) enzymes only cleave the basic residues lysine or arginine. The A forms have slightly different specificities, with Carboxypeptidase A1 (CPA1) preferring aliphatic and small aromatic residues, and CPA2 preferring the bulky aromatic side chains. Enzymes belonging to the N/E subfamily enzymes are not produced as inactive precursors and instead rely on their substrate specificity and subcellular compartmentalization to prevent inappropriate cleavages. They contain an extra C-terminal transthyretin-like domain, thought to be involved in folding or formation of oligomers. MCPs can also be classified based on their involvement in specific physiological processes; the pancreatic MCPs participate only in alimentary digestion and include carboxypeptidase A and B (A/B subfamily), while others, namely regulatory MCPs or the N/E subfamily, are involved in more selective reactions, mainly in non-digestive tissues and fluids, acting on blood coagulation/fibrinolysis, inflammation and local anaphylaxis, pro-hormone and neuropeptide processing, cellular response and others. Another MCP subfamily, is that of succinylglutamate desuccinylase /aspartoacylase, which hydrolyzes N-acetyl-L-aspartate (NAA), and deficiency in which is the established cause of Canavan disease. Another subfamily (referred to as subfamily C) includes an exceptional type of activity in the MCP family, that of dipeptidyl-peptidase activity of gamma-glutamyl-(L)-meso-diaminopimelate peptidase I which is involved in bacterial cell wall metabolism.


Pssm-ID: 349450 [Multi-domain]  Cd Length: 239  Bit Score: 46.53  E-value: 1.84e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755537116  11 KKIAIFGGTHGNELTGVFLVTHWLRNGTE--VHRAGLDVKPFItNPRAVEKCTRYIDC--DLNRVFDLENLSKEmsedlp 86
Cdd:cd06231   43 PRVLISAGIHGDEPAGVEALLRFLESLAEkyLRRVNLLVLPCV-NPWGFERNTRENADgiDLNRSFLKDSPSPE------ 115

                         90       100       110
                 ....*....|....*....|....*....|..
gi 755537116  87 yevRRAQeINHLFgpknSDDAYDLVFDLHNTT 118
Cdd:cd06231  116 ---VRAL-MEFLA----SLGRFDLHLDLHEDW 139
M14_CP_Csd4-like cd06243
Peptidase M14 carboxypeptidase Csd4 and similar proteins; This family includes peptidase M14 ...
 7-115 2.59e-06

Peptidase M14 carboxypeptidase Csd4 and similar proteins; This family includes peptidase M14 carboxypeptidase Csd4 from H. pylori which has been shown to be DL-carboxypeptidase with a modified zinc binding site containing a glutamine residue in place of a conserved histidine. It is an archetype of a new carboxypeptidase subfamily with a domain arrangement that differs from this family of peptide-cleaving enzymes. Csd4 plays a role in trimming uncrosslinked peptidoglycan peptide chains by cleaving the amide bond between meso-diaminopimelate and iso-D-glutamic acid in truncated peptidoglycan side chains. It acts as a cell shape determinant, similar to Campylobacter jejuni Pgp1. The M14 family of metallocarboxypeptidases (MCPs), also known as funnelins, are zinc-binding carboxypeptidases (CPs) which hydrolyze single, C-terminal amino acids from polypeptide chains, and have a recognition site for the free C-terminal carboxyl group, which is a key determinant of specificity. Two major subfamilies of the M14 family, defined based on sequence and structural homology, are the A/B and N/E subfamilies. Enzymes belonging to the A/B subfamily are normally synthesized as inactive precursors containing preceding signal peptide, followed by an N-terminal pro-region linked to the enzyme; these proenzymes are called procarboxypeptidases. The A/B enzymes can be further divided based on their substrate specificity; Carboxypeptidase A-like (CPA-like) enzymes favor hydrophobic residues while carboxypeptidase B-like (CPB-like) enzymes only cleave the basic residues lysine or arginine. The A forms have slightly different specificities, with Carboxypeptidase A1 (CPA1) preferring aliphatic and small aromatic residues, and CPA2 preferring the bulky aromatic side chains. Enzymes belonging to the N/E subfamily enzymes are not produced as inactive precursors and instead rely on their substrate specificity and subcellular compartmentalization to prevent inappropriate cleavage. They contain an extra C-terminal transthyretin-like domain, thought to be involved in folding or formation of oligomers. MCPs can also be classified based on their involvement in specific physiological processes; the pancreatic MCPs participate only in alimentary digestion and include carboxypeptidase A and B (A/B subfamily), while others, namely regulatory MCPs or the N/E subfamily, are involved in more selective reactions, mainly in non-digestive tissues and fluids, acting on blood coagulation/fibrinolysis, inflammation and local anaphylaxis, pro-hormone and neuropeptide processing, cellular response and others. Another MCP subfamily, is that of succinylglutamate desuccinylase /aspartoacylase, which hydrolyzes N-acetyl-L-aspartate (NAA), and deficiency in which is the established cause of Canavan disease. Another subfamily (referred to as subfamily C) includes an exceptional type of activity in the MCP family, that of dipeptidyl-peptidase activity of gamma-glutamyl-(L)-meso-diaminopimelate peptidase I which is involved in bacterial cell wall metabolism.


Pssm-ID: 349462  Cd Length: 227  Bit Score: 45.81  E-value: 2.59e-06
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755537116   7 KEPIKKIAIFGGTHGNE----LTGVFLVTHWLRNGTevhragLDVKPFItNPRAVEKCTRYIDCDLNRVFDLENLSKems 82
Cdd:cd06243   13 REPGPTLLIIGGIQGDEpggfLAADLLADLYLVKGN------VIVVPRL-NFPSILRNHRGLNGDMNRKFAALDKKD--- 82

                         90       100       110
                 ....*....|....*....|....*....|...
gi 755537116  83 edlpYEVRRAQEINHLFGPKNSddayDLVFDLH 115
Cdd:cd06243   83 ----PEYKTIQEIKSLIADFRP----DVVLHLH 107
M14_MpaA-like cd06904
Peptidase M14-like domain of Escherichia coli Murein Peptide Amidase A and related proteins; ...
 11-80 3.52e-06

Peptidase M14-like domain of Escherichia coli Murein Peptide Amidase A and related proteins; Peptidase M14-like domain of Escherichia coli Murein Peptide Amidase A (MpaA) and related proteins. MpaA is a member of the M14 family of metallocarboxypeptidases (MCPs), however it has an exceptional type of activity, it hydrolyzes the gamma-D-glutamyl-meso-diaminopimelic acid (gamma-D-Glu-Dap) bond in murein peptides. MpaA is specific for cleavage of the gamma-D-Glu-Dap bond of free murein tripeptide; it may also cleave murein tetrapeptide. MpaA has a different substrate specificity and cellular role than endopeptidase I, ENP1 (ENP1 does not belong to this group). MpaA works on free murein peptide in the recycling pathway.


Pssm-ID: 349475 [Multi-domain]  Cd Length: 214  Bit Score: 45.34  E-value: 3.52e-06
                         10        20        30        40        50        60        70
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*..
gi 755537116  11 KKIAIFGGTHGNELTGVFLVTHWLRNGTEVHRAG---LDVKPfITNPRAVEKCTRY----IdcDLNRVFDLENLSKE 80
Cdd:cd06904   24 ARILIIGGIHGDEPEGVSLVEHLLRWLKNHPASGdfhIVVVP-CLNPDGLAAGTRTnangV--DLNRNFPTKNWEPD 97
M14_ASTE cd03855
Peptidase M14 Succinylglutamate desuccinylase (ASTE) subfamily; Peptidase M14 ...
 18-115 2.35e-05

Peptidase M14 Succinylglutamate desuccinylase (ASTE) subfamily; Peptidase M14 Succinylglutamate desuccinylase (ASTE, also known as N-succinyl-L-glutamate amidohydrolase, N2-succinylglutamate desuccinylase, and SGDS; EC 3.5.1.96) belongs to the Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA) subfamily of the M14 family of metallocarboxypeptidases. This group includes succinylglutamate desuccinylase that catalyzes the fifth and last step in arginine catabolism by the arginine succinyltransferase pathway. It hydrolyzes N-succinyl-L-glutamate to succinate and L-glutamate.


Pssm-ID: 349428  Cd Length: 239  Bit Score: 43.35  E-value: 2.35e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755537116  18 GTHGNELTGVFLVTHWLRNgteVHRAGLDVKP---FI-TNPRAVEKCTRYIDCDLNRVFDLENLSKEMSedlpYEVRRAQ 93
Cdd:cd03855   51 GIHGNETAPIEILDQLIND---LIRGELALAHrllFIfGNPPAIRQGKRFIEENLNRLFSGRHSKLPPS----YETARAA 123

                         90       100
                 ....*....|....*....|....*....
gi 755537116  94 EI----NHLFgpknsDDAYDLV---FDLH 115
Cdd:cd03855  124 ELeqavADFF-----AKASGEVrwhLDLH 147
COG3608 COG3608
Predicted deacylase [General function prediction only];
7-121 3.87e-05

Predicted deacylase [General function prediction only];


Pssm-ID: 442826 [Multi-domain]  Cd Length: 296  Bit Score: 42.91  E-value: 3.87e-05
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755537116   7 KEPIKKIAIFGGTHGNELTGVFLVTHWLRNGTEVHRAG-LDVKPFItNPRAVEKCTRY--IDC-DLNRVF-DLENLSkem 81
Cdd:COG3608   23 AGPGPTLLITAGIHGDELNGIEALRRLLRELDPGELRGtVILVPVA-NPPGFLQGSRYlpIDGrDLNRSFpGDADGS--- 98

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|
gi 755537116  82 sedlpYEVRRAQEINHLFGPKnsddaYDLVFDLHNTTSNM 121
Cdd:COG3608   99 -----LAERIAHALFEEILPD-----ADYVIDLHSGGIAR 128
M14_ASTE_ASPA-like cd06256
Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA)-like; ...
 20-139 1.23e-04

Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA)-like; uncharacterized subgroup; A functionally uncharacterized subgroup of the Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA) subfamily which is part of the M14 family of metallocarboxypeptidases. ASTE catalyzes the fifth and last step in arginine catabolism by the arginine succinyltransferase pathway, and aspartoacylase (ASPA, also known as aminoacylase 2, and ACY-2; EC:3.5.1.15) cleaves N-acetyl L-aspartic acid (NAA) into aspartate and acetate. NAA is abundant in the brain, and hydrolysis of NAA by ASPA may help maintain white matter. ASPA is an NAA scavenger in other tissues. Mutations in the gene encoding ASPA cause Canavan disease (CD), a fatal progressive neurodegenerative disorder involving dysmyelination and spongiform degeneration of white matter in children. This enzyme binds zinc which is necessary for activity. Measurement of elevated NAA levels in urine is used in the diagnosis of CD.


Pssm-ID: 349474  Cd Length: 204  Bit Score: 41.13  E-value: 1.23e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755537116  20 HGNELTGVFLVTHWLRNGTEvhRAGLDVKPFITNPRAVEKCTRYID--CDLNRVFDlenlskemSEDLPYEVRRAQEINH 97
Cdd:cd06256   44 HGNEPTGLRAVQRLLKTGQA--PLPRTLLLFIGNVDAAKAGVRRLPgqPDYNRCWP--------GPFETPEGRLAAAVLE 113

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*...
gi 755537116  98 LF---GPknsddayDLVFDLHNTTSN---MGCtLILEDSRNDFLIQMF 139
Cdd:cd06256  114 RLdtlRP-------FASIDIHNNTGKnphYAC-VNRLDAAHLRLASLF 153
M14_ASTE_ASPA-like cd06251
Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA)-like; ...
 7-72 6.89e-04

Peptidase M14 Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA)-like; uncharacterized subgroup; A functionally uncharacterized subgroup of the Succinylglutamate desuccinylase (ASTE)/aspartoacylase (ASPA) subfamily which is part of the M14 family of metallocarboxypeptidases. ASTE catalyzes the fifth and last step in arginine catabolism by the arginine succinyltransferase pathway, and aspartoacylase (ASPA, also known as aminoacylase 2, and ACY-2; EC:3.5.1.15) cleaves N-acetyl L-aspartic acid (NAA) into aspartate and acetate. NAA is abundant in the brain, and hydrolysis of NAA by ASPA may help maintain white matter. ASPA is an NAA scavenger in other tissues. Mutations in the gene encoding ASPA cause Canavan disease (CD), a fatal progressive neurodegenerative disorder involving dysmyelination and spongiform degeneration of white matter in children. This enzyme binds zinc which is necessary for activity. Measurement of elevated NAA levels in urine is used in the diagnosis of CD.


Pssm-ID: 349469 [Multi-domain]  Cd Length: 195  Bit Score: 38.68  E-value: 6.89e-04
                         10        20        30        40        50        60
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....
gi 755537116   7 KEPIKKIAIFGGTHGNELTGVFLVTHWLRNGTEVHRAGLDVKPFITNPRAVEKCTRYI---DCDLNRVF 72
Cdd:cd06251    9 AKPGPTLLLTAAIHGDELNGIEVIQRLLEDLDPSKLRGTLIAIPVVNPLGFENNSRYLpddGRDLNRSF 77
PRK05324 PRK05324
succinylglutamate desuccinylase; Provisional
 18-115 9.53e-04

succinylglutamate desuccinylase; Provisional


Pssm-ID: 235408  Cd Length: 329  Bit Score: 38.62  E-value: 9.53e-04
                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 755537116  18 GTHGNELTGVFLVTHWLR---NGTEVHRAGLDVkpFITNPRAVEKCTRYIDCDLNRVFDlenlSKEMSEDLPYEVRRAQE 94
Cdd:PRK05324  55 GIHGNETAPIELLDQLVRdllAGELPLRARLLV--ILGNPPAMRAGKRYLDEDLNRLFG----GRHQQFPGSDEARRAAE 128

                         90       100
                 ....*....|....*....|....*
gi 755537116  95 INHL----FgpKNSDDAYDLVFDLH 115
Cdd:PRK05324 129 LEQAvedfF--AAGAERVRWHYDLH 151
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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