NCBI Conserved Domain Search

Conserved domains on [gi|767912484|ref|XP_011542513|]

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E3 ubiquitin-protein ligase TRIM17 isoform X2 [Homo sapiens]

Protein Classification

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List of domain hits

Name

Accession

Description

Interval

E-value

SPRY_PRY_TRIM17

cd15812

PRY/SPRY domain of tripartite motif-binding protein 17 (TRIM17), also known as testis RING ...

269-445

1.45e-136

PRY/SPRY domain of tripartite motif-binding protein 17 (TRIM17), also known as testis RING finger protein (terf); This domain, consisting of the distinct N-terminal PRY subdomain followed by the SPRY subdomain, is found at the C-terminus of TRIM17, also known as RING finger protein 16 (RNF16) or testis RING finger protein (terf). TRIM17 domain is composed of RING/B-box/coiled-coil core and also known as RBCC protein, expressed almost exclusively in the testis. It exhibits E3 ligase activity, causing protein degradation of ZW10 interacting protein (ZWINT), a known component of the kinetochore complex required for the mitotic spindle checkpoint, and negatively regulates proliferation of breast cancer cells. TRIM17 undergoes ubiquitination in COS7 fibroblast-like cells but is inhibited and stabilized by TRIM44.

Pssm-ID: 293984 [Multi-domain] Cd Length: 176 Bit Score: 389.63 E-value: 1.45e-136

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484 269 DVVPDATSAYPYLLLYESRQRRYLGSSPEGsGFCSKDRFVAYPCAVGQTAFSSGRHYWEVGMNITGDALWALGVCRDNVS 348
Cdd:cd15812    1 DVVPDPSTAYPYLLLYESRQRRYLSTPPDG-TPCSKDRFLAYPCAVGQETFSSGRHYWEVGMNLTGDALWALGVCRDNVS 79

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484 349 RKDRVPKCPENGFWVVQLSKGTKYLSTFSALTPVMLMEPPSHMGIFLDFEAGEVSFYSVSDGSHLHTYSQATFPGPLQPF 428
Cdd:cd15812   80 RKDRVPKSPENGFWVVQLSKGKKYLSAMSALTPVTLTEPPSHMGIFLDFEAGEVSFYSVNDGSHLHTYSQAAFPGPLQPF 159

                        170
                 ....*....|....*..
gi 767912484 429 FCLGAPKSGQMVISTVT 445
Cdd:cd15812  160 FCLGAPKSGQMVISTVT 176

RING_Ubox super family

cl17238

RING finger (Really Interesting New Gene) domain and U-box domain superfamily; The RING finger ...

1-51

5.50e-28

RING finger (Really Interesting New Gene) domain and U-box domain superfamily; The RING finger is a specialized type of Zn-finger of 40 to 60 residues that binds two atoms of zinc. It is defined by the "cross-brace" motif that chelates zinc atoms by eight amino acid residues, typically Cys or His, arranged in a characteristic spacing. Canonical RING motifs have been categorized into two major subclasses, RING-HC (C3HC4-type) and RING-H2 (C3H2C3-type), according to their Cys/His content. There are also many variants of RING fingers: some have different Cys/His patterns while some lack a single Cys or His residue at typical Zn ligand positions (the fourth or eighth zinc ligand is prevalently exchanged for an Asp, which can indeed chelate Zn in a RING finger as well). C4C4-, C3HC3D-, C2H2C4-, and C3HC5-type RING fingers are closely related to RING-HC fingers. In contrast, C4HC3- (RING-CH alias RINGv), C3H3C2-, C3H2C2D-, C3DHC3-, and C4HC2H-type RING fingers are more closely related to RING-H2 fingers. However, not all RING finger-containing proteins display regular RING finger features, and the RING finger family has turned out to be multifarious. The degenerate RING fingers of the Siz/PIAS RING (SP-RING) family proteins and sporulation protein RMD5, are characterized by lacking the second, fifth, and sixth Zn2+ ion-coordinating residues. They bind only one Zn2+ ion. On the other hand, the RING fingers of the human APC11 and RBX1 proteins can bind a third Zn atom since they harbor four additional Zn ligands. U-box is a modified form of the RING finger domain that lacks metal chelating Cys and His residues. It resembles the cross-brace RING structure consisting of three beta-sheets and a single alpha-helix, which would be stabilized by salt bridges instead of chelated metal ions. U-box proteins are widely distributed among eukaryotic organisms and show a higher prevalence in plants than in other organisms. RING finger/U-box-containing proteins are a group of diverse proteins with a variety of cellular functions, including oncogenesis, development, viral replication, signal transduction, the cell cycle and apoptosis. Many of them are ubiquitin-protein ligases (E3s) that serve as scaffolds for binding to ubiquitin-conjugating enzymes (E2s, also referred to as ubiquitin carrier proteins or UBCs) in close proximity to substrate proteins, which enable efficient transfer of ubiquitin from E2 to the substrates.

The actual alignment was detected with superfamily member cd16595:

Pssm-ID: 473075 [Multi-domain] Cd Length: 70 Bit Score: 105.84 E-value: 5.50e-28

                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|.
gi 767912484   1 MTTCGHNFCRACIQLSWEKARGKKGRRKRKGSFPCPECREMSPQRNLLPNR 51
Cdd:cd16595   20 MTTCGHNFCRACIQLSWEKARGKKGRRKQKGSFPCPECREMSPQRNLRPNR 70

Bbox2_TRIM7-like

cd19762

B-box-type 2 zinc finger found in tripartite motif-containing proteins TRIM7, TRIM27 and ...

71-113

1.77e-19

B-box-type 2 zinc finger found in tripartite motif-containing proteins TRIM7, TRIM27 and similar proteins; The family includes TRIM7 and TRIM27, both of which belong to the C-IV subclass of TRIM (tripartite motif) family of proteins that are defined by an N-terminal RBCC (RING, Bbox, and coiled coil) domains, including three consecutive zinc-binding domains, a RING finger, Bbox2, and a coiled coil region, as well as a B30.2/SPRY (SplA and ryanodine receptor) domain positioned C-terminal to the RBCC domain. The type 2 B-box (Bbox2) zinc finger is characterized by a CHC3H2 zinc-binding consensus motif. TRIM7, also known as glycogenin-interacting protein (GNIP) or RING finger protein 90 (RNF90), is an E3 ubiquitin-protein ligase that mediates c-Jun/AP-1 activation by Ras signalling. Its phosphorylation and activation by MSK1 in response to direct activation by the Ras-Raf-MEK-ERK pathway can stimulate TRIM7 E3 ubiquitin ligase activity in mediating Lys63-linked ubiquitination of the AP-1 coactivator RACO-1, leading to RACO-1 protein stabilization. Moreover, TRIM7 binds and activates glycogenin, the self-glucosylating initiator of glycogen biosynthesis. TRIM27, also termed RING finger protein 76 (RNF76), or RET finger protein (RFP), or zinc finger protein RFP, is a nuclear E3 ubiquitin-protein ligase that is highly expressed in testis and in various tumor cell lines. Expression of TRIM27 is associated with prognosis of colon and endometrial cancers. TRIM27 was first identified as a fusion partner of the RET receptor tyrosine kinase. It functions as a transcriptional repressor and associates with several proteins involved in transcriptional activity, such as enhancer of polycomb 1 (Epc1), a member of the Polycomb group proteins, and Mi-2beta, a main component of the nucleosome remodeling and deacetylase (NuRD) complex, and the cell cycle regulator retinoblastoma protein (RB1). It also interacts with HDAC1, leading to downregulation of thioredoxin binding protein 2 (TBP-2), which inhibits the function of thioredoxin. Moreover, TRIM27 mediates Pax7-induced ubiquitination of MyoD in skeletal muscle atrophy. It also inhibits muscle differentiation by modulating serum response factor (SRF) and Epc1. Furthermore, TRIM27 promotes non-canonical polyubiquitination of PTEN, a lipid phosphatase that catalyzes PtdIns(3,4,5)P3 (PIP3) to PtdIns(4,5)P2 (PIP2). It is an IKKepsilon-interacting protein that regulates IkappaB kinase (IKK) function and negatively regulates signaling involved in the antiviral response and inflammation. In addition, TRIM27 forms a protein complex with MBD4 or MBD2 or MBD3, and thus plays an important role in the enhancement of transcriptional repression through MBD proteins in tumorigenesis, spermatogenesis, and embryogenesis. It is also a component of an estrogen receptor 1 (ESR1) regulatory complex, and is involved in estrogen receptor-mediated transcription in MCF-7 cells. Meanwhile, TRIM27 interacts with the hinge region of chromosome 3 protein (SMC3), a component of the multimeric cohesin complex that holds sister chromatids together and prevents their premature separation during mitosis.

Pssm-ID: 380820 [Multi-domain] Cd Length: 44 Bit Score: 81.20 E-value: 1.77e-19

                         10        20        30        40
                 ....*....|....*....|....*....|....*....|...
gi 767912484  71 LCQEHHEPLKLFCQKDQSPICVVCRESREHRLHRVLPAEEAVQ 113
Cdd:cd19762    2 VCEKHQEPLKLFCKEDKRPICVVCDRSREHRHHTVLPVEEAAQ 44

Name

Accession

Description

Interval

E-value

SPRY_PRY_TRIM17

cd15812

PRY/SPRY domain of tripartite motif-binding protein 17 (TRIM17), also known as testis RING ...

269-445

1.45e-136

Pssm-ID: 293984 [Multi-domain] Cd Length: 176 Bit Score: 389.63 E-value: 1.45e-136

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484 269 DVVPDATSAYPYLLLYESRQRRYLGSSPEGsGFCSKDRFVAYPCAVGQTAFSSGRHYWEVGMNITGDALWALGVCRDNVS 348
Cdd:cd15812    1 DVVPDPSTAYPYLLLYESRQRRYLSTPPDG-TPCSKDRFLAYPCAVGQETFSSGRHYWEVGMNLTGDALWALGVCRDNVS 79

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484 349 RKDRVPKCPENGFWVVQLSKGTKYLSTFSALTPVMLMEPPSHMGIFLDFEAGEVSFYSVSDGSHLHTYSQATFPGPLQPF 428
Cdd:cd15812   80 RKDRVPKSPENGFWVVQLSKGKKYLSAMSALTPVTLTEPPSHMGIFLDFEAGEVSFYSVNDGSHLHTYSQAAFPGPLQPF 159

                        170
                 ....*....|....*..
gi 767912484 429 FCLGAPKSGQMVISTVT 445
Cdd:cd15812  160 FCLGAPKSGQMVISTVT 176

SPRY

pfam00622

SPRY domain; SPRY Domain is named from SPla and the RYanodine Receptor and it is found in many ...

323-439

1.71e-36

SPRY domain; SPRY Domain is named from SPla and the RYanodine Receptor and it is found in many eukaryotic proteins with a wide range of functions. It is a protein-interaction module involved in many important signalling pathways like RNA processing, regulation of histone H3 methylation, innate immunity or embryonic development. It can be divided into 11 subfamilies based on amino acid sequence similarity or the presence of additional protein domains. The greater SPRY family is divided into the SPRY/B30.2 (which contains a PRY extension at the N-terminal) and SPRY-only sub-families which are preceded by a subdomain that is structurally similar to the PRY region. SPRY/B30.2 structures revealed a bent beta-sandwich fold comprised of two beta-sheets. Distant homologs are domains in butyrophilin/ marenostrin/pyrin.

Pssm-ID: 459877 Cd Length: 121 Bit Score: 130.16 E-value: 1.71e-36

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484  323 RHYWEVGMNITGDALWALGVCRDNVSRKDRVPKCPENGFWVVQLSKGTKYLSTFSALTPVMLMEPPSHMGIFLDFEAGEV 402
Cdd:pfam00622   1 RHYFEVEIFGQDGGGWRVGWATKSVPRKGERFLGDESGSWGYDGWTGKKYWASTSPLTGLPLFEPGDVIGCFLDYEAGTI 80

                          90       100       110
                  ....*....|....*....|....*....|....*..
gi 767912484  403 SFYSVSdGSHLHTYSQATFPGPLQPFFCLGAPKSGQM 439
Cdd:pfam00622  81 SFTKNG-KSLGYAFRDVPFAGPLFPAVSLGAGEGLKF 116

SPRY

smart00449

Domain in SPla and the RYanodine Receptor; Domain of unknown function. Distant homologues are ...

321-441

2.14e-29

Domain in SPla and the RYanodine Receptor; Domain of unknown function. Distant homologues are domains in butyrophilin/marenostrin/pyrin homologues.

Pssm-ID: 214669 Cd Length: 122 Bit Score: 111.23 E-value: 2.14e-29

                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484   321 SGRHYWEVgmNITGDALWALGVCRDNVSRKDRVPKCPENGFWVVQLSKGTKYLSTFSALTPVMLMEPPSHMGIFLDFEAG 400
Cdd:smart00449   1 SGRHYFEV--EIGDGGHWRVGVATKSVPRGYFALLGEDKGSWGYDGDGGKKYHNSTGPEYGLPLQEPGDVIGCFLDLEAG 78

                           90       100       110       120
                   ....*....|....*....|....*....|....*....|.
gi 767912484   401 EVSFYSVSDGSHLHTYSQATFPGPLQPFFCLGAPKSGQMVI 441
Cdd:smart00449  79 TISFYKNGKYLHGLAFFDVKFSGPLYPAFSLGSGNSVRLNF 119

RING-HC_TRIM17_C-IV

cd16595

RING finger, HC subclass, found in tripartite motif-containing protein TRIM17 and similar ...

1-51

5.50e-28

RING finger, HC subclass, found in tripartite motif-containing protein TRIM17 and similar proteins; TRIM17, also known as RING finger protein 16 (RNF16) or testis RING finger protein (Terf), is a crucial E3 ubiquitin ligase that is necessary and sufficient for neuronal apoptosis and contributes to Mcl-1 ubiquitination in cerebellar granule neurons (CGNs). It interacts in a SUMO-dependent manner with nuclear factor of activated T cell NFATc3 transcription factor, and thus inhibits the activity of NFATc3 by preventing its nuclear localization. In contrast, it binds to and inhibits NFATc4 transcription factor in a SUMO-independent manner. Moreover, TRIM17 stimulates degradation of kinetochore protein ZW10 interacting protein (ZWINT), a known component of the kinetochore complex required for the mitotic spindle checkpoint, and negatively regulates cell proliferation. TRIM17 belongs to the C-IV subclass of the TRIM (tripartite motif) family of proteins that are defined by their N-terminal RBCC (RING, Bbox, and coiled coil) domains, including three consecutive zinc-binding domains, a C3HC4-type RING-HC finger, Bbox1 and Bbox2, and a coiled coil region, as well as a B30.2/SPRY (SplA and ryanodine receptor) domain positioned C-terminal to the RBCC domain.

Pssm-ID: 438257 [Multi-domain] Cd Length: 70 Bit Score: 105.84 E-value: 5.50e-28

                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|.
gi 767912484   1 MTTCGHNFCRACIQLSWEKARGKKGRRKRKGSFPCPECREMSPQRNLLPNR 51
Cdd:cd16595   20 MTTCGHNFCRACIQLSWEKARGKKGRRKQKGSFPCPECREMSPQRNLRPNR 70

Bbox2_TRIM7-like

cd19762

B-box-type 2 zinc finger found in tripartite motif-containing proteins TRIM7, TRIM27 and ...

71-113

1.77e-19

Pssm-ID: 380820 [Multi-domain] Cd Length: 44 Bit Score: 81.20 E-value: 1.77e-19

                         10        20        30        40
                 ....*....|....*....|....*....|....*....|...
gi 767912484  71 LCQEHHEPLKLFCQKDQSPICVVCRESREHRLHRVLPAEEAVQ 113
Cdd:cd19762    2 VCEKHQEPLKLFCKEDKRPICVVCDRSREHRHHTVLPVEEAAQ 44

BBOX

smart00336

B-Box-type zinc finger;

67-107

2.02e-08

B-Box-type zinc finger;

Pssm-ID: 197662 [Multi-domain] Cd Length: 42 Bit Score: 50.03 E-value: 2.02e-08

                           10        20        30        40
                   ....*....|....*....|....*....|....*....|..
gi 767912484    67 QKQDLCQEHH-EPLKLFCQKDQSPICVVCRESrEHRLHRVLP 107
Cdd:smart00336   1 QRAPKCDSHGdEPAEFFCEECGALLCRTCDEA-EHRGHTVVL 41

zf-B_box

pfam00643

B-box zinc finger;

67-107

6.51e-08

B-box zinc finger;

Pssm-ID: 459886 [Multi-domain] Cd Length: 42 Bit Score: 48.62 E-value: 6.51e-08

                          10        20        30        40
                  ....*....|....*....|....*....|....*....|..
gi 767912484   67 QKQDLCQEHH-EPLKLFCQKDQSPICVVCRESrEHRLHRVLP 107
Cdd:pfam00643   1 SKERLCPEHEeEPLTLYCNDCQELLCEECSVG-EHRGHTVVP 41

zf-C3HC4

pfam00097

Zinc finger, C3HC4 type (RING finger); The C3HC4 type zinc-finger (RING finger) is a ...

1-38

2.63e-03

Zinc finger, C3HC4 type (RING finger); The C3HC4 type zinc-finger (RING finger) is a cysteine-rich domain of 40 to 60 residues that coordinates two zinc ions, and has the consensus sequence: C-X2-C-X(9-39)-C-X(1-3)-H-X(2-3)-C-X2-C-X(4-48)-C-X2-C where X is any amino acid. Many proteins containing a RING finger play a key role in the ubiquitination pathway.

Pssm-ID: 395049 [Multi-domain] Cd Length: 40 Bit Score: 35.41 E-value: 2.63e-03

                          10        20        30
                  ....*....|....*....|....*....|....*...
gi 767912484    1 MTTCGHNFCRACIQLSWEKargkkgrrkrkGSFPCPEC 38
Cdd:pfam00097  14 LLPCGHLFCSKCIRSWLES-----------GNVTCPLC 40

Name

Accession

Description

Interval

E-value

SPRY_PRY_TRIM17

cd15812

PRY/SPRY domain of tripartite motif-binding protein 17 (TRIM17), also known as testis RING ...

269-445

1.45e-136

Pssm-ID: 293984 [Multi-domain] Cd Length: 176 Bit Score: 389.63 E-value: 1.45e-136

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484 269 DVVPDATSAYPYLLLYESRQRRYLGSSPEGsGFCSKDRFVAYPCAVGQTAFSSGRHYWEVGMNITGDALWALGVCRDNVS 348
Cdd:cd15812    1 DVVPDPSTAYPYLLLYESRQRRYLSTPPDG-TPCSKDRFLAYPCAVGQETFSSGRHYWEVGMNLTGDALWALGVCRDNVS 79

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484 349 RKDRVPKCPENGFWVVQLSKGTKYLSTFSALTPVMLMEPPSHMGIFLDFEAGEVSFYSVSDGSHLHTYSQATFPGPLQPF 428
Cdd:cd15812   80 RKDRVPKSPENGFWVVQLSKGKKYLSAMSALTPVTLTEPPSHMGIFLDFEAGEVSFYSVNDGSHLHTYSQAAFPGPLQPF 159

                        170
                 ....*....|....*..
gi 767912484 429 FCLGAPKSGQMVISTVT 445
Cdd:cd15812  160 FCLGAPKSGQMVISTVT 176

SPRY_PRY_C-I_1

cd13733

PRY/SPRY domain in tripartite motif-containing (TRIM) proteins, including TRIM5, TRIM6, TRIM7, ...

269-442

6.90e-70

PRY/SPRY domain in tripartite motif-containing (TRIM) proteins, including TRIM5, TRIM6, TRIM7, TRIM10, TRIM11, TRIM17, TRIM20, TRIM21, TRIM27, TRIM35, TRIM38, TRIM41, TRIM50, TRIM58, TRIM60, TRIM62, TRIM69, TRIM72, NF7 and bloodthirsty; This domain, consisting of the distinct N-terminal PRY subdomain followed by the SPRY subdomain, is found at the C-terminus of several Class IV TRIM proteins, including TRIM7, TRIM35, TRIM41, TRIM50, TRIM62, TRIM69, TRIM72, TRIM protein NF7 and bloodthirsty (bty). TRIM7 interacts with glycogenin and stimulates its self-glucosylating activity via its SPRY domain. TRIM35 may play a role as a tumor suppressor and is implicated in the cell death mechanism. TRIM41 is localized to speckles in the cytoplasm and nucleus, and functions as an E3 ligase that catalyzes the ubiquitin-mediated degradation of protein kinase C. TRIM50, an E3 ubiquitin ligase, is deleted in Williams-Beuren (WBS) syndrome, a multi-system neurodevelopmental disorder caused by the deletion of contiguous genes at chromosome region 7q11.23. TRIM62 is involved in the morphogenesis of the mammary gland; loss of TRIM62 gene expression in breast is associated with increased risk of recurrence in early-onset breast cancer. TRIM69 is a novel testis E3 ubiquitin ligase that may function to ubiquitinate its particular substrates during spermatogenesis. In humans, TRIM69 localizes in the cytoplasm and nucleus, and requires an intact RING finger domain to function. TRIM protein NF7, which also contains a chromodomain (CHD) at the N-terminus and an RFP (Ret finger protein)-like domain at the C-terminus, is required for its association with transcriptional units of RNA polymerase II which is mediated by a trimeric B box. In Xenopus oocyte, xNF7 has been identified as a nuclear microtubule-associated protein (MAP) whose microtubule-bundling activity, but not E3-ligase activity, contributes to microtubule organization and spindle integrity. Bloodthirsty (bty) is a novel gene identified in zebrafish and has been shown to likely play a role in in regulation of the terminal steps of erythropoiesis. TRIM72 has been shown to perform a critical function in membrane repair following acute muscle injury by nucleating the assembly of the repair machinery at injury sites. The PRY-SPRY domain in these TRIM families is suggested to serve as the target binding site.

Pssm-ID: 293968 [Multi-domain] Cd Length: 174 Bit Score: 219.27 E-value: 6.90e-70

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484 269 DVVPDATSAYPYLLLYESRQRRYLGSSPEGSGFCSKdRFVAYPCAVGQTAFSSGRHYWEVGMNitGDALWALGVCRDNVS 348
Cdd:cd13733    1 DVTLDPDTAHPNLILSEDLKSVRYGDKRQNLPDNPE-RFDTCVCVLGSEGFSSGRHYWEVEVG--GKTDWDLGVARESVN 77

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484 349 RKDRVPKCPENGFWVVQLSKGTKYLSTFSALTPVMLMEPPSHMGIFLDFEAGEVSFYSVSDGSHLHTYSQaTFPGPLQPF 428
Cdd:cd13733   78 RKGKITLSPENGYWTVGLRNGNEYKALTSPSTPLSLREKPQKVGVFLDYEEGQVSFYNVDDGSHIYTFTD-CFTEKLYPY 156

                        170
                 ....*....|....*...
gi 767912484 429 FCLGAPKSGQ----MVIS 442
Cdd:cd13733  157 FSPCLNDGGKnsapLIIC 174

SPRY_PRY_BTN1_2

cd15819

butyrophilin subfamily member A1 and A2 (BTN1A and BTN2A); This domain, consisting of the ...

269-441

1.80e-61

butyrophilin subfamily member A1 and A2 (BTN1A and BTN2A); This domain, consisting of the distinct N-terminal PRY subdomain followed by the SPRY subdomain, is found at the C-terminus of butyrophilin family 1A and 2A (BTN1A and BTN2A). BTNs belong to receptor glycoproteins of immunoglobulin (Ig) superfamily, characterized by the presence of extracellular Ig-like domains (IgV and/or IgC). BTN1A plays a role in the secretion, formation and stabilization of milk fat globules. The B30.2 domain of BTN1A1 binds the enzyme xanthine oxidoreductase (XOR) in order to participate in milk fat globule secretion; this interaction may lead to the production of reactive oxygen species, which have immunomodulatory and antimicrobial functions. Duplication events have led to three paralogs of BTN2A in primates: BTN2A1, BTN2A2, and BTN2A3. In humans, only BTN2A1 has been functionally characterized; it has been detected on epithelial cells and leukocytes, and identified as a novel ligand of dendritic cell-specific ICAM-3 grabbing nonintegrin (DCSIGN), a C-type lectin receptor that acts as an internalization receptor for HIV-1, HCV, and other pathogens. BTN2A2 mRNA has been shown to be expressed in circulating human immune cells.

Pssm-ID: 293991 [Multi-domain] Cd Length: 172 Bit Score: 197.45 E-value: 1.80e-61

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484 269 DVVPDATSAYPYLLLYESRQRRYLGSSPEGsgFCSK-DRFVAYPCAVGQTAFSSGRHYWEVGMNITGDalWALGVCRDNV 347
Cdd:cd15819    3 NVTLDPDTAHPALILSEDGRSVTWGETRQD--LPENpERFDSLPCVLGQEGFTSGRHYWEVEVGDRTS--WDLGVCRDNV 78

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484 348 SRKDRVPKCPENGFWVVQLSKGtKYLSTFSALTPVMLMEPPSHMGIFLDFEAGEVSFYSVSDGSHLHTYSQATFPGPLQP 427
Cdd:cd15819   79 MRKGRVTLSPENGFWAIRLYGN-EYWALTSPETPLTLKEPPRRVGIFLDYEAGDVSFYNMTDGSHIYTFPQTAFSGPLRP 157

                        170
                 ....*....|....
gi 767912484 428 FFCLGAPKSGQMVI 441
Cdd:cd15819  158 FFRLWSSDSGPLTI 171

SPRY_PRY_TRIM39

cd13745

PRY/SPRY domain in tripartite motif-binding protein 39 (TRIM39) and TRIM39-like; This domain, ...

266-432

1.70e-53

PRY/SPRY domain in tripartite motif-binding protein 39 (TRIM39) and TRIM39-like; This domain, consisting of the distinct N-terminal PRY subdomain followed by the SPRY subdomain, is found at the C-terminus of pyrin, several tripartite motif-containing proteins (TRIMs), including E3 ubiquitin-protein ligase (TRIM21), RET finger protein (RFP)/tripartite motif protein 27 (TRIM27), as well as butyrophilin (Btns) and butyrophilin-like (Btnl) family members, with the exception of Btnl2. Btn and Btnl family members are novel regulators of immune responses, with many of the genes located within the MHC. They are implicated in T-cell inhibition and modulation of epithelial cell-T cell interactions. TRIM21 (also known as RO52, SSA1 or RNF81) is a major autoantigen in autoimmune diseases such as rheumatoid arthritis, systemic lupus erythematosus, and Sjorgen's syndrome. TRIM27 (also known as Ret finger protein, RFP or RNF76) negatively regulates CD4 T-cells by ubiquitinating and inhibiting the class II phosphatidylinositol 3 kinase C2beta (PI3K-C2beta), a kinase critical for KCa3.1 channel activation. The PRY/SPRY domain of Pyrin, which is mutated in familial Mediterranean fever patients, interacts with inflammasome components and inhibits proIL-1beta processing.

Pssm-ID: 293979 [Multi-domain] Cd Length: 177 Bit Score: 177.05 E-value: 1.70e-53

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484 266 FLEDVVPDATSAYPYLLLYESR-------QRRYLGSSPEgsgfcskdRFVAYPCAVGQTAFSSGRHYWEVGMnitGDAL- 337
Cdd:cd13745    1 FAVDVTLDPDTAHPNLVLSEDRksvrhgdTRQDLPDNPE--------RFDTYPCVLGAEGFTGGRHYWEVEV---GDKTe 69

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484 338 WALGVCRDNVSRKDRVPKCPENGFWVVQLSKGtKYLSTFSALTPVMLMEPPSHMGIFLDFEAGEVSFYSVSDGSHLHTYS 417
Cdd:cd13745   70 WTLGVCRESVSRKGEVTLSPENGYWTVWLRDG-KYEALTSPPTPLPVSVRPSRVGIFLDYEAGEVSFYNVTDRSHLFTFT 148

                        170
                 ....*....|....*
gi 767912484 418 QaTFPGPLQPFFCLG 432
Cdd:cd13745  149 D-TFSGTLRPYFYPG 162

SPRY_PRY_TRIM20

cd15813

PRY/SPRY domain in tripartite motif-binding protein 20 (TRIM20), also known as pyrin; This ...

269-438

3.12e-46

PRY/SPRY domain in tripartite motif-binding protein 20 (TRIM20), also known as pyrin; This domain, consisting of the distinct N-terminal PRY subdomain followed by the SPRY subdomain, is found at the C-terminus of TRIM20, which is also known as pyrin or marenostrin. Unlike TRIM domains that are composed of RING/B-box/coiled-coil core, the N-terminal RING domain in TRIM20 is exchanged by a PYRIN domain (PYD), a prime mediator of protein interactions necessary for apoptosis, inflammation and innate immune signaling pathway, and it also harbors a C-terminal B30.2 domain. Mutations in pyrin (TRIM20) are associated with familial Mediterranean fever (FMF), a recessively hereditary periodic fever syndrome, characterized by episodes of inflammation and fever. These mutations cluster in the C-terminal B30.2 domain and therefore it is assumed that pyrin plays a role in the innate immune system by possibly effecting caspase-1-dependent IL-1beta maturation.

Pssm-ID: 293985 Cd Length: 184 Bit Score: 158.00 E-value: 3.12e-46

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484 269 DVVPDATSAYPYLLLYESRQRRYLGSS----PEGSGfcskdRFVAYPCAVGQTAFSSGRHYWEVGMnitGDAL-WALGVC 343
Cdd:cd15813   10 NVTLDPETAHPNLIFSDDLKSVRLGNKwdrlPDNPE-----RFDSCIIVLGSPSFTSGRHYWEVEV---GDKTgWILGVC 81

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484 344 RDNVSRKDRVPKCPENGFWVVQLSKGTKYLSTFSALTPVMLMEPPSHMGIFLDFEAGEVSFYSVSDGSHLHTYSQATFPG 423
Cdd:cd15813   82 KASVSRKGSMTLSPENGYWVVMMTKRNEYQASTSPPTRLWLREPPRRVGIFLDYEAGDISFYNVTAKSHIYTFTSFSSSG 161

                        170
                 ....*....|....*
gi 767912484 424 PLQPFFCLGAPKSGQ 438
Cdd:cd15813  162 PLQPIFSPGTHDGGK 176

SPRY_PRY_RFPL

cd15821

Ret finger protein-like (RFPL), includes RFP1, 2, 3, 4; This domain, consisting of the ...

266-444

3.00e-45

Ret finger protein-like (RFPL), includes RFP1, 2, 3, 4; This domain, consisting of the distinct N-terminal PRY subdomain followed by the SPRY subdomain, is found at the C-terminus of RFPL protein family, which includes RFPL1, RFPL2, RFPL3 and RFPL4. In humans, RFPL transcripts can be detected at the onset of neurogenesis in differentiating human embryonic stem cells, and in the developing human neocortex. The human RFPL1, 2, 3 genes have a role in neocortex development. RFPL1 is a primate-specific target gene of Pax6, a key transcription factor for pancreas, eye and neocortex development; human RFPL1 decreases cell number through its RFPL-defining motif (RDM) and SPRY domains. The RFPL4 (also known as RFPL4A) gene encodes a putative E3 ubiquitin-protein ligase expressed in adult germ cells and interacts with oocyte proteins of the ubiquitin-proteasome degradation pathway.

Pssm-ID: 293993 [Multi-domain] Cd Length: 178 Bit Score: 155.55 E-value: 3.00e-45

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484 266 FLEDVVPDATSAYPYLLLYESRQRRYLGSSPEGSGFCSkDRFVAYPCAVGQTAFSSGRHYWEVGmnITGDALWALGVCRD 345
Cdd:cd15821    2 FQVDMTLDVDTANNYLIISEDLRSVRCGCFRQNRKELA-ERFDDALCVLGSPRFTSGRHYWEVD--VGTSTEWDLGVCRE 78

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484 346 NVSRKDRVPKCPENGFWVVQLSKGTKYLSTFSALTPVMLMEPPSHMGIFLDFEAGEVSFYSVSDGSHLHTYSQATFPGPL 425
Cdd:cd15821   79 SVNRQGPIELSPEHGFWTVSLRDGSVFFASTVPLTVLWVNPRLHRVGIFLDMEMGTISFYDVSDGSHIFTFTKISAEEPL 158

                        170
                 ....*....|....*....
gi 767912484 426 QPFFCLGAPKSGQMVISTV 444
Cdd:cd15821  159 RPFFAPANPYGDDQGVLSI 177

SPRY_PRY_TRIM58

cd15816

PRY/SPRY domain in tripartite motif-binding protein 58 (TRIM58), also known as BIA2; This ...

269-434

4.92e-45

PRY/SPRY domain in tripartite motif-binding protein 58 (TRIM58), also known as BIA2; This domain, consisting of the distinct N-terminal PRY subdomain followed by the SPRY subdomain, is found at the C-terminus of TRIM58, also known as BIA2. TRIM58 domains are composed of RING/B-box/coiled-coil core and also known as RBCC proteins.It is implicated by genome-wide association studies (GWAS) to regulate erythrocyte traits, including cell size and number. Trim58 facilitates erythroblast enucleation by inducing proteolytic degradation of the microtubule motor dynein.

Pssm-ID: 293988 [Multi-domain] Cd Length: 168 Bit Score: 154.56 E-value: 4.92e-45

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484 269 DVVPDATSAYPYLLLYESRQRRYLGSSPEGSGfCSKDRFVAYPCAVGQTAFSSGRHYWEVGMnitGD-ALWALGVCRDNV 347
Cdd:cd15816    1 DVKLDPATAHPSLLLTADLRSVQDGELWRDVP-GNPERFDTWPCVLGLQSFSSGRHYWEVAV---GEkAEWGLGVCQDSA 76

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484 348 SRKDRVPKCPENGFWVVQLSKGTKYLSTFSALTPVMLMEPPSHMGIFLDFEAGEVSFYSVSDGSHLHTYSQaTFPGPLQP 427
Cdd:cd15816   77 PRKGETTPSPENGVWAVWLLKGNEYMVLASPSVPLLQLRRPRRVGVFLDYEAGEISFYNVTAGSHIYTFRQ-LFSGILRP 155


                 ....*....
gi 767912484 428 FF--CLGAP 434
Cdd:cd15816  156 YFfvCDTTP 164

SPRY_PRY_BTN3

cd15820

PRY/SPRY domain of butyrophilin 3 (BTN3), includes BTN3A1, BTN3A2, BTN3A3 as well as BTN-like ...

268-429

5.50e-45

PRY/SPRY domain of butyrophilin 3 (BTN3), includes BTN3A1, BTN3A2, BTN3A3 as well as BTN-like 3 (BTNL3); BTN3A also known as CD277; This domain, consisting of the distinct N-terminal PRY subdomain followed by the SPRY subdomain, is found at the C-terminus of butyrophilin family 3A (BTN3A); duplication events have led to three paralogs in primates: BTN3A1, BTN3A2, and BTN3A3. BTNs belong to receptor glycoproteins of immunoglobulin (Ig) superfamily, characterized by the presence of extracellular Ig-like domains (IgV and/or IgC). BTN3 transcripts are ubiquitously present in all immune cells (T cells, B cells, NK cells, monocytes, dendritic cells, and hematopoietic precursors) with different expression levels; BTN3A1 and BTN3A2 are expressed mainly by CD4+ and CD8+ T cells, BTN3A2 is the major form expressed in NK cells, and BTN3A3 is poorly expressed in these immune cells. The PRY/SPRY domain of the BTN3A1 isoform mediates phosphoantigen (pAg)-induced activation by binding directly to the pAg.

Pssm-ID: 293992 [Multi-domain] Cd Length: 176 Bit Score: 154.51 E-value: 5.50e-45

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484 268 EDVVPDATSAYPYLLLYESRQRRYLGSSPEGSGFCSKdRFVAYPCAVGQTAFSSGRHYWEVGMnitGD-ALWALGVCRDN 346
Cdd:cd15820    4 ADVILDPDTANPILLISEDQRSLQWADEPQNLPDNPK-RFDWHYCVLGCKSFTSGRHFWEVEV---GDrKEWYVGVCREN 79

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484 347 VSRKDRVPKCPENGFWVVQLSKGTKYLSTFSALTPVMLMEPPSHMGIFLDFEAGEVSFYSVSDGSHLHTYSQATFPGPLQ 426
Cdd:cd15820   80 VERKLWVKMAPENGFWTIGLSDGNDYQALTDPRTKLTIANPPQRVGVFLDYETGEVSFYNAMDGSHIYTFPHTSFSGPLY 159


                 ...
gi 767912484 427 PFF 429
Cdd:cd15820  160 PVF 162

SPRY_PRY_TRIM38

cd15815

PRY/SPRY domain of tripartite motif-binding protein 38 (TRIM38), also known as Ring finger ...

263-430

2.68e-44

PRY/SPRY domain of tripartite motif-binding protein 38 (TRIM38), also known as Ring finger protein 15 (RNF15); This domain, consisting of the distinct N-terminal PRY subdomain followed by the SPRY subdomain, is found at the C-terminus of TRIM38, which is also known as RING finger protein 15 (RNF15) or RORET. TRIM38 domains are composed of RING/B-box/coiled-coil core and also known as RBCC proteins. TRIM38 has been shown to act as a suppressor in TOLL-like receptor (TLR)-mediated interferon (IFN)-beta induction by promoting degradation of TRAF6 and NAP1 through the ubiquitin-proteasome system. Another study has shown that TRIM38 may act as a novel negative regulator for TLR3-mediated IFN-beta signaling by targeting TRIF for degradation. TRIM38 has been identified as a critical negative regulator in TNFalpha- and IL-1beta-triggered activation of NF-kappaB and MAP Kinases (MAPKs); it causes degradation of two essential cellular components, TGFbeta-associated kinase 1 (TAK1)-associating chaperones 2 and 3 (TAB2/3). The degradation is promoted through a lysosomal-dependent pathway, which requires the C-terminal PRY-SPRY of TRIM38. Enterovirus 71 infection induces degradation of TRIM38, suggesting that TRIM38 may play a role in viral infections.

Pssm-ID: 293987 [Multi-domain] Cd Length: 182 Bit Score: 152.89 E-value: 2.68e-44

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484 263 LRGFLEDVVPDATSAYPYLLLYESRQRRYLGSSPEGSGFCSKdRFVAYPCAVGQTAFSSGRHYWEVGM-NITGdalWALG 341
Cdd:cd15815    8 LRRHQVSVTLDPDTAHPELTLSKDQRQVTYGRCQENLDASPK-RFTVLPCVLGCEGFTSGRHYFEVDVgEGTG---WDVG 83

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484 342 VCRDNVSRKDRVPKCPENGFWVVQLSKGTKYLSTFSALTPVMLMEPPSHMGIFLDFEAGEVSFYSVSDGSHLHTYSQATF 421
Cdd:cd15815   84 VCLENVQRGFGMKQEPEFGFWTIRLCEEDGYVALTSPPTPLPLREKPLVVGVFLDYEAGLVSFYNMTTGSHIFTFPKASF 163


                 ....*....
gi 767912484 422 PGPLQPFFC 430
Cdd:cd15815  164 SDTLRPYFQ 172

SPRY_PRY_TRIM21

cd12900

PRY/SPRY domain in tripartite motif-binding protein 21 (TRIM21) also known as 52kD ...

273-437

3.18e-42

PRY/SPRY domain in tripartite motif-binding protein 21 (TRIM21) also known as 52kD Ribonucleoprotein Autoantigen (Ro52); This domain, consisting of the distinct N-terminal PRY subdomain followed by the SPRY subdomain, is found at the C-terminus of TRIM21, which is also known as Sjogren Syndrome Antigen A (SSA), SSA1, 52kD Ribonucleoprotein Autoantigen (Ro52, Ro/SSA, SS-A/Ro) or RING finger protein 81 (RNF81). TRIM21 domains are composed of RING/B-box/coiled-coil core and also known as RBCC proteins. As an E3 ligase, TRIM21 mediates target specificity in ubiquitination; it regulates type 1 interferon and proinflammatory cytokines via ubiquitination of interferon regulatory factors (IRFs). It is up-regulated at the site of autoimmune inflammation, such as cutaneous lupus lesions, indicating a central role in the tissue destructive inflammatory process. It interacts with auto-antigens in patients with Sjogren syndrome and systemic lupus erythematosus, a chronic systemic autoimmune disease characterized by the presence of autoantibodies against the protein component of the human intracellular ribonucleoprotein-RNA complexes and more specifically TRIM21, Ro60/TROVE2 and La/SSB proteins. It binds the Fc part of IgG molecules via its PRY-SPRY domain with unexpectedly high affinity.

Pssm-ID: 293957 Cd Length: 180 Bit Score: 147.34 E-value: 3.18e-42

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484 273 DATSAYPYLLLYESRQRRYLGSSPEGSGFcSKDRFVAYPCAVGQTAFSSGRHYWEVgmNITGDALWALGVCRDNVSRKDR 352
Cdd:cd12900    8 DPDTANPWLILSKDRRQVRLGDTHQNVPE-NEERFDNYPMVLGAQRFNSGKHYWEV--DVTGKEAWDLGVCRDSVRRKGQ 84

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484 353 VPKCPENGFWVVQLSKGtKYLSTFSALTPVMLMEPPSHMGIFLDFEAGEVSFYSVSD-GSHLHTYSQATFPGPLQPFFCL 431
Cdd:cd12900   85 FLLSPENGFWTIWLWNK-KYEAGTSPQTTLHLQVPPCQVGIFLDYEAGVVSFYNITDhGSLIYTFSECAFTGPLRPFFNP 163


                 ....*.
gi 767912484 432 GAPKSG 437
Cdd:cd12900  164 GFNDSG 169

SPRY_PRY_TRIM75

cd15829

PRY/SPRY domain of tripartite motif-binding protein 75 (TRIM75); This domain, consisting of ...

261-432

1.32e-41

PRY/SPRY domain of tripartite motif-binding protein 75 (TRIM75); This domain, consisting of the distinct N-terminal PRY subdomain followed by the SPRY subdomain, is found at the C-terminus of TRIM75, also known as Gm794. TRIM75 domains are composed of RING/B-box/coiled-coil core and also known as RBCC proteins. TRIM75 has a single site of positive selection in its RING domain associated with E3 ubiquitin ligase activity. It has not been detectably expressed experimentally due to their constant turnover by the proteasome, and therefore not been characterized.

Pssm-ID: 294001 Cd Length: 187 Bit Score: 145.89 E-value: 1.32e-41

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484 261 EVLRGFLEDVVPDATSAYPYLLLYESRQR-RYLGSSPEGSGFcsKDRFVAYPCAVGQTAFSSGRHYWEVGMnitGD-ALW 338
Cdd:cd15829   12 KIIKKFRVDVTLDPETAHPNLLVSEDKKCvTFTKKKQRVPDS--PKRFTVNPVVLGFPGFHSGRHFWEVEV---GDkPEW 86

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484 339 ALGVCRDNVSRKDRVPKCPENGFWVVQLSKGTkYLSTFSALTPVMLMEPPSHMGIFLDFEAGEVSFYSVSDGSHLHTYSQ 418
Cdd:cd15829   87 AVGVCKDSLSTKARRPPSGQQGCWRIQLQGGD-YDAPGAVPPPLLLEVKPRGIGVFLDYELGEISFYNMPEKSHIHTFTD 165

                        170
                 ....*....|....
gi 767912484 419 aTFPGPLQPFFCLG 432
Cdd:cd15829  166 -TFSGPLRPYFYVG 178

SPRY_PRY_TRIM27

cd15814

PRY/SPRY domain in tripartite motif-containing protein 27 (TRIM27), also known as RING finger ...

269-431

1.58e-40

PRY/SPRY domain in tripartite motif-containing protein 27 (TRIM27), also known as RING finger protein 76 (RNF76); This domain, consisting of the distinct N-terminal PRY subdomain followed by the SPRY subdomain, is found at the C-terminus of TRIM27, also known as RING finger protein 76 (RNF76) or RET finger protein (RFP). TRIM27 domain is composed of RING/B-box/coiled-coil core and also known as RBCC proteins. It is highly expressed in the spleen, thymus and in cells of the hematopoietic compartment. TRIM27 exhibits either nuclear or cytosolic localization depending on the cell type. TRIM27 negatively regulates nucleotide-binding oligomerization domain containing 2 (NOD2)-mediated signaling by proteasomal degradation of NOD2, suggesting that TRIM27 could be a new target for therapeutic intervention in NOD2-associated diseases such as Crohn's. High expression of TRIM27 is observed in several human cancers, including breast and endometrial cancer, where elevated TRIM27 expression predicts poor prognosis. Also, TRIM27 forms an oncogenic fusion protein with Ret proto-oncogene. It is involved in different stages of spermatogenesis and its significant expression in male germ cells and seminomas, suggests that TRIM27 may be associated with the regulation of testicular germ cell proliferation and histological-type of germ cell tumors. TRIM27 could also be a predictive marker for chemoresistance in ovarian cancer patients. In the neurotoxin model of Parkinson's disease (PD), deficiency of TRIM27 decreases apoptosis and protects dopaminergic neurons, making TRIM27 an effective potential target during the treatment of PD.

Pssm-ID: 293986 [Multi-domain] Cd Length: 177 Bit Score: 142.91 E-value: 1.58e-40

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484 269 DVVPDATSAYPYLLLYES-RQRRY------LGSSPEgsgfcskdRFVAYPCAVGQTAFSSGRHYWEVgmNITGDALWALG 341
Cdd:cd15814    3 DVTLDPDTAYPSLILSDNlRQVRYsylqqdLPDNPE--------RFNLFPCVLGSPCFIAGRHYWEV--EVGDKAKWTIG 72

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484 342 VCRDNVSRKDRVPKCPENGFWVVQLSKGTKYLSTFSALTPVMLMEPPSHMGIFLDFEAGEVSFYSVSDGSHLHTYSQATF 421
Cdd:cd15814   73 VCEDSVCRKGGVTSAPQNGFWAVSLWYGKEYWALTSPMTALPLRTPLQRVGIFLDYDAGEVSFYNVTERCHTFTFSHATF 152

                        170
                 ....*....|
gi 767912484 422 PGPLQPFFCL 431
Cdd:cd15814  153 CGPVRPYFSL 162

SPRY_PRY_TRIM11

cd15811

PRY/SPRY domain of tripartite motif-binding protein 11 (TRIM11), also known as RING finger ...

269-429

4.65e-40

PRY/SPRY domain of tripartite motif-binding protein 11 (TRIM11), also known as RING finger protein 92 (RNF92); This domain, consisting of the distinct N-terminal PRY subdomain followed by the SPRY subdomain, is found at the C-terminus of TRIM11, also known as RING finger protein 92 (RNF92) or BIA1. TRIM11 domains are composed of RING/B-box/coiled-coil core and also known as RBCC proteins. It localizes to the nucleus and the cytoplasm; it is overexpressed in high-grade gliomas and promotes proliferation, invasion, migration and glial tumor growth. TRIM11 increases expression of dopamine beta-hydroxylase gene by interacting with the homeodomain transcription factor, PHOX2B, via the B30.2/SPRY domain, thus playing a potential role in the specification of noradrenergic (NA) neuron phenotype. It has also been shown that TRIM11 plays a critical role in the clearance of mutant PHOX2B, which causes congenital central hypoventilation syndrome, via the proteasome. TRIM11 binds a key component of the activator-mediated cofactor complex (ARC105), and destabilizes it, through the ubiquitin-proteasome system; ARC105 mediates chromatin-directed transcription activation and is a key regulatory factor for transforming growth factor beta (TGFbeta) signaling.

Pssm-ID: 293983 [Multi-domain] Cd Length: 169 Bit Score: 141.25 E-value: 4.65e-40

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484 269 DVVPDATSAYPYLLLYESR-------QRRYLGSSPEgsgfcskdRFVAYPCAVGQTAFSSGRHYWEVGMnitGD-ALWAL 340
Cdd:cd15811    1 DVTLDPDTANPELVLSEDRrsvrrgdLRQALPDSPE--------RFDPGPCVLGRERFTSGRHYWEVEV---GDrTSWAL 69

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484 341 GVCRDNVSRKDRVPKCPENGFWVVQLSkGTKYLSTFSALTPvmLMEPPSHMGIFLDFEAGEVSFYSVSDGSHLHTYSQAT 420
Cdd:cd15811   70 GVCKENVNRKEKGELSAGNGFWILVFL-GNYYSSERRTFAP--LRDPPRRVGIFLDYEAGHLSFYSATDGSLLFIFPETP 146


                 ....*....
gi 767912484 421 FPGPLQPFF 429
Cdd:cd15811  147 FSGTLRPLF 155

SPRY

pfam00622

SPRY domain; SPRY Domain is named from SPla and the RYanodine Receptor and it is found in many ...

323-439

1.71e-36

Pssm-ID: 459877 Cd Length: 121 Bit Score: 130.16 E-value: 1.71e-36

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484  323 RHYWEVGMNITGDALWALGVCRDNVSRKDRVPKCPENGFWVVQLSKGTKYLSTFSALTPVMLMEPPSHMGIFLDFEAGEV 402
Cdd:pfam00622   1 RHYFEVEIFGQDGGGWRVGWATKSVPRKGERFLGDESGSWGYDGWTGKKYWASTSPLTGLPLFEPGDVIGCFLDYEAGTI 80

                          90       100       110
                  ....*....|....*....|....*....|....*..
gi 767912484  403 SFYSVSdGSHLHTYSQATFPGPLQPFFCLGAPKSGQM 439
Cdd:pfam00622  81 SFTKNG-KSLGYAFRDVPFAGPLFPAVSLGAGEGLKF 116

SPRY_PRY_TRIM60_75

cd15817

PRY/SPRY domain of tripartite motif-binding protein 60 and 75 (TRIM60 and TRIM75); This domain, ...

269-442

3.25e-35

PRY/SPRY domain of tripartite motif-binding protein 60 and 75 (TRIM60 and TRIM75); This domain, consisting of the distinct N-terminal PRY subdomain followed by the SPRY subdomain, is found at the C-terminus of TRIM60 and TRIM75, both composed of RING/B-box/coiled-coil core and also known as RBCC proteins. TRIM60 domain is also known as RING finger protein 33 (RNF33) or 129 (RNF129). Based on its expression profile, RNF33 likely plays an important role in the spermatogenesis process, the development of the pre-implantation embryo, and in testicular functions; Rnf33 is temporally transcribed in the unfertilized egg and the pre-implantation embryo, and is permanently silenced before the blastocyst stage. Mice experiments have shown that RNF33 associates with the cytoplasmic motor proteins, kinesin-2 family members 3A (KIF3A) and 3B (KIF3B), suggesting possible contribution to cargo movement along the microtubule in the expressed sites. TRIM75, also known as Gm794, has a single site of positive selection in its RING domain associated with E3 ubiquitin ligase activity. It has not been detectably expressed experimentally due to their constant turnover by the proteasome, and therefore not been characterized.

Pssm-ID: 293989 [Multi-domain] Cd Length: 168 Bit Score: 128.44 E-value: 3.25e-35

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484 269 DVVPDATSAYPYLLLYESRQR-RYLGSSPEGSGFcsKDRFVAYPCAVGQTAFSSGRHYWEVGMNITGDalWALGVCRDNV 347
Cdd:cd15817    1 DLILDPETAHPNLIVSEDRKAvRYRRMKPNCPYD--PRRFTVYPAVLGSEGFDSGRHFWEVEVGGKGE--WILGVCKDSL 76

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484 348 SRKDRVPKCPENGFWVVQLSKgTKYLSTFSALTPVMLMEPPSHMGIFLDFEAGEVSFYSVSDGSHLHTYSQaTFPGPLQP 427
Cdd:cd15817   77 PRNAQDPPSPLGGCWQIGRYM-SGYVASGPKTTQLLPVVKPSRIGIFLDYELGEVSFYNMNDRSHLYTFTD-TFTGKLIP 154

                        170
                 ....*....|....*
gi 767912484 428 FFCLGaPKSGQMVIS 442
Cdd:cd15817  155 YFYVG-PDSEPLTIC 168

SPRY_PRY_TRIM35

cd12893

PRY/SPRY domain in tripartite motif-containing protein 35 (TRIM35); This PRY/SPRY domain is ...

270-436

6.03e-32

PRY/SPRY domain in tripartite motif-containing protein 35 (TRIM35); This PRY/SPRY domain is found at the C-terminus of the overall domain architecture of tripartite motif 35, TRIM35 (also known as hemopoietic lineage switch protein), which includes a RING finger domain (RING) and a B-box motif (BBOX). TRIM35 may play a role as a tumor suppressor and is implicated in the cell death mechanism.

Pssm-ID: 293950 [Multi-domain] Cd Length: 171 Bit Score: 119.66 E-value: 6.03e-32

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484 270 VVPDATSAYPYLLLYE-------SRQRRYLGSSPEgsgfcskdRFVAYPCAVGQTAFSSGRHYWEVGmniTGD-ALWALG 341
Cdd:cd12893    2 VTLDPNTAHPWLSLSEdltsvrySSEKQQLPDNPE--------RFDPYPCVLGSEGFTSGKHSWDVE---VGDnTSWMLG 70

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484 342 VCRDNVSRKDRVPKCPENGFWVVQLSKGtKY--LSTFSALTPVMLMEPPSHMGIFLDFEAGEVSFYSVSDGSHLHTYSqA 419
Cdd:cd12893   71 VAKESVQRKGKFTLSPESGFWTIGFSEG-KYsaRTSPEPRTPLRVKQKPQRIRVQLDWDRGKVSFSDPDTNTHIHTFT-H 148

                        170
                 ....*....|....*..
gi 767912484 420 TFPGPLQPFFCLGAPKS 436
Cdd:cd12893  149 TFTERVFPYFYTGCKSE 165

SPRY_PRY_TRIM7_like

cd12888

PRY/SPRY domain in tripartite motif-binding protein 7 (TRIM7)-like, including TRIM7, TRIM10, ...

269-432

3.76e-31

PRY/SPRY domain in tripartite motif-binding protein 7 (TRIM7)-like, including TRIM7, TRIM10, TRIM15, TRIM26, TRIM39, TRIM41; This domain, consisting of the distinct N-terminal PRY subdomain followed by the SPRY subdomain, is found at the C-terminus of several tripartite motif-containing (TRIM) proteins, including TRIM7 (also referred to as glycogenin-interacting protein, RING finger protein 90 or RNF90), TRIM10, TRIM15, TRIM26, TRIM39 and TRIM41. TRIM7 or GNIP interacts with glycogenin and stimulates its self-glucosylating activity via its SPRY domain. TRIM10 (also known as hematopoietic RING finger 1 (HERF1) or TRIM10/HERF1) plays a key role in definitive erythroid development; downregulation of the Spi-1/PU.1 oncogene induces the expression of TRIM10/HERF1, a key factor required for terminal erythroid cell differentiation and survival. Antiviral activity of TRIM15 is dependent on the ability of its B-box to interact with the MLV Gag precursor protein; downregulation of TRIM15, along with TRIM11, enhances virus release suggesting that these proteins contribute to the endogenous restriction of retroviruses in cells. Tripartite motif-containing 26 (TRIM26) function is as yet unknown; however, since it is localized in the human histocompatibility complex (MHC) class I region, TRIM26 may play a role in immune response although studies show no association between TRIM26 polymorphisms and the risk of aspirin-exacerbated respiratory disease. TRIM39 is a MOAP-1 (Modulator of Apoptosis)-binding protein that stabilizes MOAP-1 through inhibition of its poly-ubiquitination process. TRIM41 (also known as RING finger-interacting protein with C kinase or RINCK) functions as an E3 ligase that catalyzes the ubiquitin-mediated degradation of protein kinase C.

Pssm-ID: 293946 [Multi-domain] Cd Length: 169 Bit Score: 117.66 E-value: 3.76e-31

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484 269 DVVPDATSAYPYLLLYESRQ-------RRYLGSSPEgsgfcskdRFVAYPCAVGQTAFSSGRHYWEVgmNITGDALWALG 341
Cdd:cd12888    1 NVTLDPDTAHPRLVLSEDRKsvrwgdtRQDLPDNPE--------RFDTWPCVLGCEGFTSGRHYWEV--EVGDGGGWAVG 70

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484 342 VCRDNVSRKDRVPKCPENGFWVVQLSKGtkylsTFSALT----PVMLMEPPSHMGIFLDFEAGEVSFYSVSDGSHLHTYS 417
Cdd:cd12888   71 VARESVRRKGEISFSPEEGIWAVGQWGG-----QYWALTspetPLPLSEVPRRIRVYLDYEGGQVAFFDADNEAPIFTFP 145

                        170
                 ....*....|....*.
gi 767912484 418 QATFPGP-LQPFFCLG 432
Cdd:cd12888  146 PASFAGErIFPWFWVG 161

SPRY_PRY_A33L

cd12905

zinc-binding protein A33-like; This domain, consisting of the distinct N-terminal PRY ...

273-438

6.62e-31

zinc-binding protein A33-like; This domain, consisting of the distinct N-terminal PRY subdomain followed by the SPRY subdomain, is found at the C-terminus of TRIM69 and TRIM proteins NF7 and bloodthirsty (bty). TRIM69 is a novel testis E3 ubiquitin ligase that may function to ubiquitinate its particular substrates during spermatogenesis. In humans, TRIM69 localizes in the cytoplasm and nucleus, and requires an intact RING finger domain to function. TRIM protein NF7, which also contains a chromodomain (CHD) at the N-terminus and an RFP (Ret finger protein)-like domain at the C-terminus, is required for its association with transcriptional units of RNA polymerase II which is mediated by a trimeric B box. In Xenopus oocyte, xNF7 has been identified as a nuclear microtubule-associated protein (MAP) whose microtubule-bundling activity, but not E3-ligase activity, contributes to microtubule organization and spindle integrity. Bloodthirsty (bty) is a novel gene identified in zebrafish and has been shown to likely play a role in in regulation of the terminal steps of erythropoiesis.

Pssm-ID: 293962 [Multi-domain] Cd Length: 178 Bit Score: 117.13 E-value: 6.62e-31

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484 273 DATSAYPYLLLyeSRQRRYLGSSPEGSGF-CSKDRFVAYPCAVGQTAFSSGRHYWEVGmnITGDALWALGVCRDNVSRKD 351
Cdd:cd12905    9 DPETAHPSLIL--SRDLTAVTESDEMQPYpRSPKRFLQCVNVLASQGFQSGRHYWEVW--VGSKTKWDLGVASESVDRQA 84

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484 352 RVPKCPENGFWVVQLSKGTKYLSTFSALTPVMLMEPPSHMGIFLDFEAGEVSFYSVSDGSHLHTYSQATfPGPLQPFFCL 431
Cdd:cd12905   85 RVKLCPENGYWTLRLRNGDEYWAGTQPWTRLRVTSRPQRIGVFLDCEERKVSFYNADDMSLLYSFHQGP-RGKVFPFFST 163


                 ....*..
gi 767912484 432 GAPKSGQ 438
Cdd:cd12905  164 CFSDDGQ 170

SPRY_PRY_TRIM60

cd15828

PRY/SPRY domain of tripartite motif-binding protein 60 (TRIM60) also known as RING finger ...

266-432

1.02e-29

PRY/SPRY domain of tripartite motif-binding protein 60 (TRIM60) also known as RING finger protein 33 (RNF33); This domain, consisting of the distinct N-terminal PRY subdomain followed by the SPRY subdomain, is found at the C-terminus of TRIM60, which is also known as RING finger protein 33 (RNF33) or 129 (RNF129). TRIM60 domains are composed of RING/B-box/coiled-coil core and also known as RBCC proteins. Based on its expression profile, RNF33 likely plays an important role in the spermatogenesis process, the development of the pre-implantation embryo, and in testicular functions; Rnf33 is temporally transcribed in the unfertilized egg and the pre-implantation embryo, and is permanently silenced before the blastocyst stage. Mice experiments have shown that RNF33 associates with the cytoplasmic motor proteins, kinesin-2 family members 3A (KIF3A) and 3B (KIF3B), suggesting possible contribution to cargo movement along the microtubule in the expressed sites.

Pssm-ID: 294000 [Multi-domain] Cd Length: 180 Bit Score: 113.92 E-value: 1.02e-29

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484 266 FLEDVVPDATSAYPYLLLYESRQRRYLGSSPEGSGFCSKdRFVAYPCAVGQTAFSSGRHYWEVGMnitGDA-LWALGVCR 344
Cdd:cd15828    8 FQVDVTLDPETAHPQLTVSEDRKSVLYGEMKQNVCYNPR-RFYLCPAVLGSEGFHSGRQYWEVEV---GDKpEWTLGVCQ 83

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484 345 DNVSRKDRVPKCPENGFWVVQLSKGTKYLsTF----SALTPVMLmepPSHMGIFLDFEAGEVSFYSVSDGSHLHTYSQaT 420
Cdd:cd15828   84 DCLPRNWSNQPSVQDGLWAIGRYSESNYV-ALgpkkIQLLPKVR---PSKIGIFLDYELGEVSFYNMNDRSLLYTFSD-S 158

                        170
                 ....*....|..
gi 767912484 421 FPGPLQPFFCLG 432
Cdd:cd15828  159 FTGTLWPYFYTG 170

SPRY

smart00449

Domain in SPla and the RYanodine Receptor; Domain of unknown function. Distant homologues are ...

321-441

2.14e-29

Domain in SPla and the RYanodine Receptor; Domain of unknown function. Distant homologues are domains in butyrophilin/marenostrin/pyrin homologues.

Pssm-ID: 214669 Cd Length: 122 Bit Score: 111.23 E-value: 2.14e-29

                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484   321 SGRHYWEVgmNITGDALWALGVCRDNVSRKDRVPKCPENGFWVVQLSKGTKYLSTFSALTPVMLMEPPSHMGIFLDFEAG 400
Cdd:smart00449   1 SGRHYFEV--EIGDGGHWRVGVATKSVPRGYFALLGEDKGSWGYDGDGGKKYHNSTGPEYGLPLQEPGDVIGCFLDLEAG 78

                           90       100       110       120
                   ....*....|....*....|....*....|....*....|.
gi 767912484   401 EVSFYSVSDGSHLHTYSQATFPGPLQPFFCLGAPKSGQMVI 441
Cdd:smart00449  79 TISFYKNGKYLHGLAFFDVKFSGPLYPAFSLGSGNSVRLNF 119

SPRY_PRY

cd12874

PRY/SPRY domain, also known as B30.2; This domain contains residues in the N-terminus that ...

273-432

7.32e-29

PRY/SPRY domain, also known as B30.2; This domain contains residues in the N-terminus that form a distinct PRY domain structure such that the B30.2 domain consists of PRY and SPRY subdomains. B30.2 domains comprise the C-terminus of three protein families: BTNs (receptor glycoproteins of immunoglobulin superfamily); several TRIM proteins (composed of RING/B-box/coiled-coil core); Stonutoxin (secreted poisonous protein of the stonefish Synanceia horrida). While SPRY domains are evolutionarily ancient, B30.2 domains are a more recent adaptation where the SPRY/PRY combination is a possible component of immune defense. Among the TRIM proteins, also known as the N-terminal RING finger/B-box/coiled coil (RBCC) family, only Classes I and II contain the B30.2 domain that has evolved under positive selection. Class I TRIM proteins include multiple members involved in antiviral immunity at various levels of interferon signaling cascade. Among the 75 human TRIMs, roughly half enhance immune response, which they do at multiple levels in signaling pathways. The PRY-SPRY domain in these TRIM families is suggested to serve as the target binding site.

Pssm-ID: 293934 [Multi-domain] Cd Length: 168 Bit Score: 111.24 E-value: 7.32e-29

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484 273 DATSAYPYLLLyESRQRRYLGSSPEGSGFCSKDRFVAYPCAVGQTAFSSGRHYWEVgmNITGDALWALGVCRDNVSRKDR 352
Cdd:cd12874    4 DPDTAHLNLIL-SDDLRSVRVGDISQHPPEPPPRFFECWQVLGSQSFSSGRHYWEV--DVQDDSSWYVGVTYKSLPRKGK 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484 353 VPKC-PENGFWVVQLSKGTkylstFSALT----PVMLMEPPSHMGIFLDFEAGEVSFYSVSDG-SHLHTYsQATFPGPLQ 426
Cdd:cd12874   81 MSNLgRNNGSWCLEWRENE-----FSAWHnnpeTRLPVTPPRRLGVFLDCDGGSLSFYGVTDGvQLLYTF-KAKFTEPLY 154


                 ....*.
gi 767912484 427 PFFCLG 432
Cdd:cd12874  155 PAFWLG 160

RING-HC_TRIM17_C-IV

cd16595

RING finger, HC subclass, found in tripartite motif-containing protein TRIM17 and similar ...

1-51

5.50e-28

Pssm-ID: 438257 [Multi-domain] Cd Length: 70 Bit Score: 105.84 E-value: 5.50e-28

                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|.
gi 767912484   1 MTTCGHNFCRACIQLSWEKARGKKGRRKRKGSFPCPECREMSPQRNLLPNR 51
Cdd:cd16595   20 MTTCGHNFCRACIQLSWEKARGKKGRRKQKGSFPCPECREMSPQRNLRPNR 70

SPRY_PRY_TRIM69

cd15818

PRY/SPRY domain in tripartite motif-binding protein 69 (TRIM69), also known as RING finger ...

262-430

1.08e-27

PRY/SPRY domain in tripartite motif-binding protein 69 (TRIM69), also known as RING finger protein 36 (RNF36); This domain, consisting of the distinct N-terminal PRY subdomain followed by the SPRY subdomain, is found at the C-terminus of TRIM69, which is also known as RING finger protein 36 (RNF36) or testis-specific ring finger (Trif). TRIM69 domains are composed of RING/B-box/coiled-coil core and also known as RBCC proteins. It is a novel testis E3 ubiquitin ligase that may function to ubiquitinate its particular substrates during spermatogenesis. In humans, TRIM69 localizes in the cytoplasm and nucleus, and requires an intact RING finger domain to function. The mouse ortholog of this gene is specifically expressed in germ cells at the round spermatid stages during spermatogenesis and, when overexpressed, induces apoptosis. TRIM69 has been shown to be a novel regulator of mitotic spindle assembly in tumor cells; it associates with spindle poles and promotes centrosomal clustering, and is therefore essential for formation of a bipolar spindle.

Pssm-ID: 293990 [Multi-domain] Cd Length: 187 Bit Score: 108.74 E-value: 1.08e-27

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484 262 VLRGFLEDVVPDATSAYPYLLLYESRQ-------RRYLGSSPEgsgfcskdRFVAYPCAVGQTAFSSGRHYWEVgmNITG 334
Cdd:cd15818    7 ILNPGLSLITLDPKTAHPNLILSEDLTcvwhgdtKQMLPDNPE--------RFDSSVAVLGSEGFTSGKHYWEV--EVAK 76

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484 335 DALWALGVCRDNVSRKDRVPKCPENGFWVVQLSKGTKylstFSAL-TPVM---LMEPPSHMGIFLDFEAGEVSFYSVSDG 410
Cdd:cd15818   77 KTKWTLGVVRESINRKGNCPLSPEDGFWLLRLRNQNE----LKALdVPSFsltLTSNLNKVGIYLDYEGGQVSFYNANTM 152

                        170       180
                 ....*....|....*....|
gi 767912484 411 SHLHTYSQaTFPGPLQPFFC 430
Cdd:cd15818  153 SHIYTFSD-TFTEKIYPYFC 171

SPRY_PRY_TRIM4

cd15809

PRY/SPRY domain in tripartite motif-binding protein 4 (TRIM4), also known as RING finger ...

305-431

7.03e-27

PRY/SPRY domain in tripartite motif-binding protein 4 (TRIM4), also known as RING finger protein 87 (RNF87); This domain, consisting of the distinct N-terminal PRY subdomain followed by the SPRY subdomain, is found at the C-terminus of TRIM4 which is also known as RING finger protein 87 (RNF87). TRIM4 domain is composed of RING/B-box/coiled-coil core and also known as RBCC protein. It is a positive regulator of RIG-I-mediated interferon (IFN) induction. It regulates virus-induced IFN induction and cellular antiviral innate immunity by targeting RIG-I for K63-linked poly-ubiquitination. Over-expression of TRIM4 enhances virus-triggered activation of transcription factors IRF3 and NF-kappaB, as well as IFN-beta induction. Expression of TRIM4 differs significantly in Huntington's Disease (HD) neural cells when compared with wild-type controls, possibly impacting down-regulation of the Huntingtin (HTT) gene, which is involved in the regulation of diverse cellular activities that are impaired in Huntington's Disease (HD) cells.

Pssm-ID: 293981 Cd Length: 191 Bit Score: 106.45 E-value: 7.03e-27

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484 305 DRFVAYPCAVGQTAFSSGRHYWEVGmniTGDAL-WALGVCRDNV-SRKDRVPKCPENGFWVVQLSKGTKYLSTFSALTPV 382
Cdd:cd15809   58 ERFQHLPCVLGKNVFTSGKHYWEVE---NRDSLeIAVGVCREDVmGITDGSEMSPHVGIWAICWSSAGYRPLTSSPVSPT 134

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|
gi 767912484 383 MLmEPPSH-MGIFLDFEAGEVSFYSVSDGSHLHTYSqATFPGPLQPFFCL 431
Cdd:cd15809  135 KQ-EPALHrVGVFLDHGAGEVSFYSAVDGVHLHTFS-CPLVSRLRPFFWL 182

SPRY_PRY_TRIM5_6_22_34

cd15810

PRY/SPRY domain of tripartite motif-binding protein 5, 6, 22 and 34 (TRIM5, TRIM6, TRIM22 and ...

269-429

1.90e-25

PRY/SPRY domain of tripartite motif-binding protein 5, 6, 22 and 34 (TRIM5, TRIM6, TRIM22 and TRIM34); This domain, consisting of the distinct N-terminal PRY subdomain followed by the SPRY subdomain, is found at the C-terminus of very close paralogs, TRIM5, TRIM6, TRIM22 and TRIM34. These domains are composed of RING/B-box/coiled-coil core and are also known as RBCC proteins. They form a locus of four closely related TRIM genes within an olfactory receptor-rich region on chromosome 11 of the human genome. Genetic analysis of this locus indicates that these four genes have evolved by gene duplication from a common ancestral gene. All genes in the TRIM6/TRIM34/TRIM5/TRIM22 locus are type I interferon inducible, with TRIM5 and TRIM22 possessing antiviral properties. TRIM5 promotes innate immune signaling by activating the TAK1 kinase complex by cooperating with the heterodimeric E2, UBC13/UEV1A. It also stimulates NFkB and AP-1 signaling, and the transcription of inflammatory cytokines and chemokines, amplifying these activities upon retroviral infection. Interaction of its PRY-SPRY or cyclophilin domains with the retroviral capsid lattice stimulates the formation of a complementary lattice by TRIM5, with greatly increased TRIM5 E3 activity, and host cell signal transduction. TRIM6 is selectively expressed in embryonic stem (ES) cells and interacts with the proto-oncogene product Myc, maintaining the pluripotency of the ES cells. TRIM6, together with E2 Ubiquitin conjugase (UbE2K) and K48-linked poly-Ub chains, is critical for the IkappaB kinase epsilon (IKKepsilon) branch of type I interferon (IFN-I) signaling pathway and subsequent establishment of a protective antiviral response. TRIM22 plays an integral role in the host innate immune response to viruses; it has been shown to inhibit the replication of a number of viruses, including HIV-1, hepatitis B, and influenza A. Altered TRIM22 expression has also been associated with multiple sclerosis, cancer, and autoimmune disease. While the PRY-SPRY domain of TRIM5a provides specificity and the capsid recognition motif to retroviral restriction, TRIM34 binds HIV-1 capsid but does not restrict HIV-1 infection.

Pssm-ID: 293982 [Multi-domain] Cd Length: 189 Bit Score: 102.56 E-value: 1.90e-25

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484 269 DVVPDATSAYPYLLL-YESRQRRYLGSSPegSGFCSKDRFVAYPcAVGQTAFSSGRHYWEVgmNITGDALWALGVC---R 344
Cdd:cd15810    1 DVTLNPVNISLNIVIsEDQRQVRIVPPQT--SGQALTNNNYDFG-VLGSQYFSSGKHYWEV--DVSKKSAWILGVCshkR 75

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484 345 DNVSRKDRVPKC----------PENGFWVVQLSKGTKYlSTF---SALTPVMLM----EPPSHMGIFLDFEAGEVSFYSV 407
Cdd:cd15810   76 SDAMTKSNANQInhqnvysryqPQYGYWVIGLQNESEY-NAFedsSSFNPHVLTlsvtVPPHRVGVFLDYEAGTVSFFNV 154

                        170       180
                 ....*....|....*....|...
gi 767912484 408 SD-GSHLHTYSQATFPGPLQPFF 429
Cdd:cd15810  155 TNhGSLIYKFSKCCFSTTVCPYF 177

SPRY_PRY_TRIM50_72

cd12897

PRY/SPRY domain in tripartite motif-binding (TRIM) proteins TRIM50 and TRIM72; This domain, ...

267-429

2.00e-25

PRY/SPRY domain in tripartite motif-binding (TRIM) proteins TRIM50 and TRIM72; This domain, consisting of the distinct N-terminal PRY subdomain followed by the SPRY subdomain, is found at the C-terminus of several TRIM proteins, including TRIM72 and TRIM50. TRIM72 (also known as MG53) has been shown to perform a critical function in membrane repair following acute muscle injury by nucleating the assembly of the repair machinery at injury sites. It is expressed specifically in skeletal muscle and heart, and tethered to the plasma membrane and cytoplasmic vesicles via its interaction with phosphatidylserine. TRIM50, an E3 ubiquitin ligase, is deleted in Williams-Beuren (WBS) syndrome, a multi-system neurodevelopmental disorder caused by the deletion of contiguous genes at chromosome region 7q11.23.

Pssm-ID: 293954 Cd Length: 191 Bit Score: 102.69 E-value: 2.00e-25

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484 267 LEDVVPDATSAYPYLLLYE-------SRQRRYLGSSPEgsgfcskdRFVAYPCAVGQTAFSSGRHYWEVgmnITGD-ALW 338
Cdd:cd12897   11 LESLTFDPATAHPLLVVSSggtvvecGLQKQRRASQPE--------RFDKSTCVVASQGFSEGEHYWEV---VVGDkPRW 79

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484 339 ALGVCRDNVSRKDRVPKCPENGFWVVQLSKGTKYLSTFSALTPVMLMEP--PSHMGIFLDFEAGEVSFYSVSDGSHLHT- 415
Cdd:cd12897   80 ALGVIKGTASRKGKLHASPSHGVWLIGLKEGKVYEAHGEPKEPRPLRVAgrPHRIGVYLSFEDGVLSFFDASDPDDLRTl 159

                        170
                 ....*....|....*
gi 767912484 416 YS-QATFPGPLQPFF 429
Cdd:cd12897  160 YTfQERFQGKLYPFF 174

SPRY_PRY_TRIM34

cd15825

PRY/SPRY domain in tripartite motif-containing protein 34 (TRIM34), also known as RING finger ...

314-429

2.29e-23

PRY/SPRY domain in tripartite motif-containing protein 34 (TRIM34), also known as RING finger protein 21 (RNF21) or interferon-responsive finger protein (IFP1); This domain, consisting of the distinct N-terminal PRY subdomain followed by the SPRY subdomain, is found at the C-terminus of TRIM34, also known as RING finger protein 21 (RNF21) or interferon-responsive finger protein (IFP1). TRIM34 domain is composed of RING/B-box/coiled-coil core and also known as RBCC protein. The TRIM21 cDNA possesses at least three kinds of isoforms, due to alternative splicing, of which only the long and medium forms contain the SPRY domain. It is an interferon-induced protein, predominantly expressed in the testis, kidney, and ovary. The SPRY domain provides the capsid recognition motif that dictates specificity to retroviral restriction. While the PRY-SPRY domain provides specificity and the capsid recognition motif to retroviral restriction, TRIM34 binds HIV-1 capsid but does not restrict HIV-1 infection.

Pssm-ID: 293997 Cd Length: 185 Bit Score: 96.83 E-value: 2.29e-23

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484 314 VGQTAFSSGRHYWEVgmNITGDALWALGV-CRDNVSRKDRVPKC-----------PENGFWVVQLSKGTKYL-----STF 376
Cdd:cd15825   40 LGSQYFSSGKHYWEV--DVSKKTAWILGVyCRKRSRTFKYVRQGknhpnvysryrPQYGYWVIGLQNKSEYYafedsSTS 117

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*.
gi 767912484 377 --SALTPVMLMePPSHMGIFLDFEAGEVSFYSVSD-GSHLHTYSQATFPGPLQPFF 429
Cdd:cd15825  118 dpKVLTLSVAT-PPHRVGVFLDYEAGTVSFFNVTNhGSLIYKFSKCCFSQPVYPYF 172

SPRY_PRY_C-II

cd13734

PRY/SPRY domain in tripartite motif-containing proteins 1, 9, 18, 36, 46, 67,76 (TRIM1, TRIM9, ...

273-432

5.35e-23

PRY/SPRY domain in tripartite motif-containing proteins 1, 9, 18, 36, 46, 67,76 (TRIM1, TRIM9, TRIM18, TRIM36, TRIM46, TRIM67, TRIM76); This domain, consisting of the distinct N-terminal PRY subdomain followed by the SPRY subdomain, is found at the C-terminus of several Class I TRIM proteins, including TRIM1, TRIM9, TRIM18, TRIM36, TRIM46, TRIM67 and TRIM76. TRIM1 (also known as MID2) and its close homolog, TRIM18 (also known as MID1), both contain a B30.2-like domain at their C-terminus and a single fibronectin type III (FN3) motif between it and their N-terminal RBCC domain. Their coiled-coil motifs mediate both homo- and heterodimerization, a prerequisite for association of the rapamycin-sensitive PP2A regulatory subunit Alpha 4 with microtubules. Mutations in TRIM18 have shown to cause Opitz syndrome, a disorder causing congenital anomalies such as cleft lip and palate as well as heart defects. TRIM9 is expressed mainly in the cerebral cortex, and functions as an E3 ubiquitin ligase. Its immunoreactivity is severely decreased in affected brain areas in Parkinson's disease and dementia with Lewy bodies, possibly playing an important role in the regulation of neuronal function and participating in pathological process of Lewy body disease through its ligase. TRIM36 interacts with centromere protein-H, one of the kinetochore proteins and possibly associates with chromosome segregation; an excess of TRIM36 may cause chromosomal instability. TRIM46 has not yet been characterized. TRIM67 negatively regulates Ras activity via degradation of 80K-H, leading to neural differentiation, including neuritogenesis. TRIM76 (also known as cardiomyopathy-associated protein 5 or CMYA5) is a muscle-specific member of the TRIM superfamily, but lacks the RING domain. It is possibly involved in protein kinase A signaling as well as vesicular trafficking. It has also been implicated in Duchenne muscular dystrophy and cardiac disease. The PRY-SPRY domain in these TRIM families is suggested to serve as the target binding site.

Pssm-ID: 293969 [Multi-domain] Cd Length: 166 Bit Score: 95.04 E-value: 5.35e-23

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484 273 DATSAYPYLLLyeSRQRRYLGSSPEGSGFC--SKDRFVAYPCAV-GQTAFSSGRHYWEVgmNITGDALWALGVCRDNVSR 349
Cdd:cd13734    4 DPKTAHRKLRL--SNDNLTVEYDPEGSKDQaaVLPRRFTGSPAVlGDVAISSGRHYWEV--SVSRSTSYRVGVAYKSAPR 79

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484 350 KDRVPKcpENGFWVVQLSKGTKYLSTFSALTPVMLMEPPSHMGIFLDFEAGEVSFYSVSDGSHLHTYSqATFPGPLQPFF 429
Cdd:cd13734   80 DEDLGK--NSTSWCLSRDNNRYTARHDGKVVDLRVTGHPARIGVLLDYDNGTLSFYDAESKQHLYTFH-VDFEGPVCPAF 156


                 ...
gi 767912484 430 CLG 432
Cdd:cd13734  157 AVW 159

SPRY_PRY_TRIM50

cd13743

PRY/SPRY domain in tripartite motif-binding protein 50 (TRIM50); This domain, consisting of ...

305-429

6.74e-23

PRY/SPRY domain in tripartite motif-binding protein 50 (TRIM50); This domain, consisting of the distinct N-terminal PRY subdomain followed by the SPRY subdomain, is found at the C-terminus of TRIM50. TRIM50, an E3 ubiquitin ligase, is deleted in Williams-Beuren (WBS) syndrome, a multi-system neurodevelopmental disorder caused by the deletion of contiguous genes at chromosome region 7q11.23. It is specifically expressed in gastric parietal cells and may play an essential role in tubulovesicular dynamics. It also interacts with and increases the level of p62, a multifunctional adaptor protein that is implicated in various cellular processes such as the autophagy clearance of polyubiquitinated protein aggregates.

Pssm-ID: 293977 Cd Length: 189 Bit Score: 95.64 E-value: 6.74e-23

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484 305 DRFVAYPCAVGQTAFSSGRHYWEVgmnITGD-ALWALGVCRDNVSRKDRVPKCPENGFWVVQLSKGTKYLSTFSALTPVM 383
Cdd:cd13743   48 ERFDYSNCVLASRGFSSGKHYWEV---VVGSkSKWRLGLIKGTTSRKGKLNKSPENGVWLIGLKEGRVYEAFANPRVPLP 124

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*...
gi 767912484 384 LMEPPSHMGIFLDFEAGEVSFYSVSDGSHLHT-YS-QATFPGPLQPFF 429
Cdd:cd13743  125 LSTRPQRIGVFLDYEKGELTFYNADSPDELVPiYTfQAEFQGKLYPLL 172

SPRY_PRY_TRIM15

cd15826

PRY/SPRY domain in tripartite motif-binding protein 15 (TRIM15); This domain, consisting of ...

270-436

9.06e-23

PRY/SPRY domain in tripartite motif-binding protein 15 (TRIM15); This domain, consisting of the distinct N-terminal PRY subdomain followed by the SPRY subdomain, is found at the C-terminus of tripartite motif-containing protein 15 (TRIM15), also referred to as RING finger protein 93 (RNF93) or Zinc finger protein B7 or 178 (ZNFB7 or ZNF178). TRIM15 domains are composed of RING/B-box/coiled-coil core and also known as RBCC proteins. The PRY and SPRY/B30.2 domains can function as immune defense components and in pathogen sensing. TRIM15 has been shown to regulate inflammatory and innate immune signaling, in addition to displaying antiviral activities. Down-regulation of TRIM15, as well as TRIM11, enhances virus release, suggesting that these proteins contribute to the endogenous restriction of retroviruses in cells. TRIM15 is also a regulatory component of focal adhesion turnover and cell migration.

Pssm-ID: 293998 [Multi-domain] Cd Length: 170 Bit Score: 94.55 E-value: 9.06e-23

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484 270 VVPDATSAYPYLLLYE-------SRQRRYLGSSPEgsgfcskdRFVAYPCAVGQTAFSSGRHYWEVGMNITGDALWALGV 342
Cdd:cd15826    2 VTLDPQTASGSLVLSEdrksvryTRQKQNLPDSPL--------RFDGLPAVLGSPGFSSGRHRWQVEVQLGDGGGCTVGV 73

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484 343 CRDNVSRKDRVPKCPENGFWVVQLSKGTKYLSTfSALTPVMLMEPPSHMGIFLDFEAGEVSFYSVSDGSHLHTYSqATFP 422
Cdd:cd15826   74 AGESVRRKGEMGLSAEDGVWAVILSHQQCWAST-SPGTDLPLSEIPRRVGVALDYEAGTVTLTNAETQEPIFTFT-ASFS 151

                        170
                 ....*....|....
gi 767912484 423 GPLQPFFCLGAPKS 436
Cdd:cd15826  152 GKVFPFFAVWKKGS 165

SPRY_PRY_TRIM6

cd15823

PRY/SPRY domain in tripartite motif-binding protein 6 (TRIM6), also known as RING finger ...

266-429

4.40e-22

PRY/SPRY domain in tripartite motif-binding protein 6 (TRIM6), also known as RING finger protein 89 (RNF89); This domain, consisting of the distinct N-terminal PRY subdomain followed by the SPRY subdomain, is found at the C-terminus of TRIM6, also known as RING finger protein 89 (RNF89). TRIM6 domain is composed of RING/B-box/coiled-coil core and also known as RBCC protein. It is selectively expressed in embryonic stem (ES) cells and interacts with the proto-oncogene product Myc, maintaining the pluripotency of the ES cells. TRIM6, together with E2 Ubiquitin conjugase (UbE2K) and K48-linked poly-Ub chains, is critical for the IkappaB kinase epsilon (IKKepsilon) branch of type I interferon (IFN-I) signaling pathway and subsequent establishment of a protective antiviral response.

Pssm-ID: 293995 Cd Length: 188 Bit Score: 93.39 E-value: 4.40e-22

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484 266 FLEDVVPDATSAYPYLLLYES-RQRRYLGSSPEGSGFCSKDrfvaYPCAV-GQTAFSSGRHYWEVgmNITGDALWALGVC 343
Cdd:cd15823    1 YWVDVTLNPHTANLNLVLSKNrRQVRFVGAKLSGPSYLEEH----YDCSVlGSQHFSSGKHYWEV--DVTKKTAWILGVC 74

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484 344 RDNV-----------SRKDRVPKCPENGFWVVQLSKGTKYLSTFSALTPVMLME--PPSHMGIFLDFEAGEVSFYSVSD- 409
Cdd:cd15823   75 SHSLgptfsfnqyaqNHNAYSRYQPQSGYWVIGLQHNHEYRAYEDSSTSLLLSMtvPPRRVGVFLDYEAGTVSFYNVTNh 154

                        170       180
                 ....*....|....*....|
gi 767912484 410 GSHLHTYSQATFPGPLQPFF 429
Cdd:cd15823  155 GFPIYTFSKYYFPTTLCPYF 174

SPRY_PRY_TRIM72

cd13742

PRY/SPRY domain in tripartite motif-binding protein 72 (TRIM72); This domain, consisting of ...

267-429

5.78e-21

PRY/SPRY domain in tripartite motif-binding protein 72 (TRIM72); This domain, consisting of the distinct N-terminal PRY subdomain followed by the SPRY subdomain, is found at the C-terminus of TRIM72. Muscle-specific TRIM72 (also known as Mitsugumin 53 or MG53) has been shown to perform a critical function in membrane repair following acute muscle injury by nucleating the assembly of the repair machinery at injury sites. It is expressed specifically in skeletal muscle and heart, and tethered to the plasma membrane and cytoplasmic vesicles via its interaction with phosphatidylserine. TRIM72 interacts with dysferlin, a sarcolemmal protein whose deficiency causes Miyoshi myopathy (MM) and limb girdle muscular dystrophy type 2B (LGMD2B); this coordination plays an important role in the repair of sarcolemma damage.

Pssm-ID: 293976 [Multi-domain] Cd Length: 192 Bit Score: 90.30 E-value: 5.78e-21

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484 267 LEDVVPDATSAYPYLLLYESRQRRYLGSSPEGSGFCSKDRFVAYPCAVGQTAFSSGRHYWEVgmnITGDA-LWALGVCRD 345
Cdd:cd13742   11 LENLTFDPDTAHPYLVVSSDGKRVECADQKQAVSSDDPNRFDKANCVVSHQSFSEGEHYWEV---IVGDKpRWALGVISA 87

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484 346 NVSRKDRVPKCPENGFWVVQLSKGTKYLSTFSALTPVMLMEP--PSHMGIFLDFEAGEVSFYSVSDGSHL---HTYSQaT 420
Cdd:cd13742   88 EAGRKGRLHALPSNGFWLLGCKEGKVYEAHVEHKEPRALRVEgrPTRIGVYLSFSDGVLSFYDASDEDNLvqlFAFHE-R 166


                 ....*....
gi 767912484 421 FPGPLQPFF 429
Cdd:cd13742  167 FPGPLYPFF 175

SPRY_PRY_TRIM5

cd15822

PRY/SPRY domain in tripartite motif-binding protein 5 (TRIM5), also known as RING finger ...

315-429

6.48e-21

PRY/SPRY domain in tripartite motif-binding protein 5 (TRIM5), also known as RING finger protein 88 (RNF88); This domain, consisting of the distinct N-terminal PRY subdomain followed by the SPRY subdomain, is found at the C-terminus of TRIM5 which is also known as RING finger protein 88 (RNF88) or TRIM5alpha (TRIM5a), an antiretroviral restriction factor and a retrovirus capsid sensor in immune signaling. TRIM5 domain is composed of RING/B-box/coiled-coil core and also known as RBCC protein. It blocks retrovirus infection soon after the virion core enters the cell cytoplasm by recognizing the capsid protein lattice that encases the viral genomic RNA; the SPRY domain provides the capsid recognition motif that dictates specificity to retroviral restriction. TRIM5a, an E3 ubiquitin ligase, promotes innate immune signaling by activating the TAK1 kinase complex by cooperating with the heterodimeric E2, UBC13/UEV1A. It also stimulates NFkB and AP-1 signaling, and the transcription of inflammatory cytokines and chemokines, and amplifies these activities upon retroviral infection. Interaction of its PRY-SPRY or cyclophilin domains with the retroviral capsid lattice stimulates the formation of a complementary lattice by TRIM5, with greatly increased TRIM5 E3 activity, and host cell signal transduction.

Pssm-ID: 293994 Cd Length: 200 Bit Score: 90.36 E-value: 6.48e-21

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484 315 GQTAFSSGRHYWEVgmNITGDALWALGVC----------RDNVSRKDRVPKC----PENGFWVVQLSKGTKYL----STF 376
Cdd:cd15822   55 GSPSITSGKHYWEV--DVSKKRAWILGVCggkypnstlkDFNKQGKNNQKQCsnyqPKYGYWVIGLQNKSEYNafedSSS 132

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|....*.
gi 767912484 377 SALTPVML--MEPPSHMGIFLDFEAGEVSFYSVS-DGSHLHTYSQATFPGPLQPFF 429
Cdd:cd15822  133 SDPLILTLslTVPPCRVGVFLDYEAGTVSFFNVTnHGFLIYKFSSCSFSQEVFPYF 188

SPRY_PRY_TRIM7

cd13740

PRY/SPRY domain in tripartite motif-binding protein 7 (TRIM7); This domain, consisting of the ...

269-445

3.76e-20

PRY/SPRY domain in tripartite motif-binding protein 7 (TRIM7); This domain, consisting of the distinct N-terminal PRY subdomain followed by the SPRY subdomain, is found at the C-terminus of tripartite motif-containing protein 7 (TRIM7), also referred to as glycogenin-interacting protein (GNIP) or RING finger protein 90 (RNF90). TRIM7 or GNIP interacts with glycogenin and stimulates its self-glucosylating activity via its SPRY domain. The GNIP gene encodes at least four distinct isoforms of GNIP, of which three (GNIP1, GNIP2, and GNIP3) have the B30.2 domain.

Pssm-ID: 293975 [Multi-domain] Cd Length: 169 Bit Score: 87.32 E-value: 3.76e-20

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484 269 DVVPDATSAYPYLLLYESRQRRYLGsspegsgfCSKDRFVAYPC-------AVGQTAFSSGRHYWEVGMNiTGDAlWALG 341
Cdd:cd13740    1 ELTLDPDSANPRLILSLDLKSVRLG--------ERAQDLPNHPCrfdtntrVLASCGFSSGRHHWEVEVG-SKDG-WAFG 70

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484 342 VCRDNVSRKDRVPKCPENGFWVVQLSKGTKYLSTFSALTPVMLMEpPSHMGIFLDFEAGEVSFYSVSDGSHLHTYsQATF 421
Cdd:cd13740   71 VARESVRRKGLTPFTPEEGVWALQLNGGQYWAVTSPERTPLSCGH-LSRVRVALDLEVGAVSFYAAEDMRHIYTF-RVNF 148

                        170       180
                 ....*....|....*....|....
gi 767912484 422 PGPLQPFFClgapksgqmVISTVT 445
Cdd:cd13740  149 QERVFPLFS---------VCSTGT 163

SPRY_PRY_C-I_2

cd12891

PRY/SPRY domain in tripartite motif-containing (TRIM) proteins, including TRIM14-like, ...

273-429

7.29e-20

PRY/SPRY domain in tripartite motif-containing (TRIM) proteins, including TRIM14-like, TRIM16-like, TRIM25-like, TRIM47-like, TRIM65 and RNF135, and stonustoxin; This domain, consisting of the distinct N-terminal PRY subdomain followed by the SPRY subdomain, is found at the C-terminus of several Class I TRIM proteins, including TRIM14, TRIM16 and TRIM25, TRIM47 as well as RING finger protein RNF135 and stonustoxin, a secreted poisonous protein of the stonefish Synanceja horrida. TRIM16 (also known as estrogen-responsive B box protein or EBBP) has E3 ubiquitin ligase activity. It is a regulator of keratinocyte differentiation and a tumor suppressor in retinoid-sensitive neuroblastoma. TRIM25 (also called Efp) ubiquitinates the N terminus of the viral RNA receptor retinoic acid-inducible gene-I (RIG-I) in response to viral infection, leading to activation of the RIG-I signaling pathway, thus resulting in type I interferon production to limit viral replication. It has been shown that the influenza A virus targets TRIM25 and disables its antiviral function. TRIM47, also known as GOA (Gene overexpressed in astrocytoma protein) or RNF100 (RING finger protein 100), is highly expressed in kidney tubular cells, but low expressed in most tissue. It is overexpressed in astrocytoma tumor cells and plays an important role in the process of dedifferentiation that is associated with astrocytoma tumorigenesis. RNF135 ubiquitinates RIG-I (retinoic acid-inducible gene-I) to promote interferon-beta induction during the early phase of viral infection. Stonustoxin (STNX) is a hypotensive and lethal protein factor that also possesses other biological activities such as species-specific hemolysis (due to its ability to form pores in the cell membrane) and platelet aggregation, edema-induction, and endothelium-dependent vasorelaxation (mediated by the nitric oxide pathway and activation of potassium channels). The PRY-SPRY domain in these TRIM families is suggested to serve as the target binding site.

Pssm-ID: 293949 [Multi-domain] Cd Length: 167 Bit Score: 86.15 E-value: 7.29e-20

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484 273 DATSAYPYLLLYESRqRRYLGSSPEGSGFCSKDRFvAYPCAVGQTAFSSGRHYWEVgmNITGDALWALGVCRDNVSRKDR 352
Cdd:cd12891    4 DPNTAHNNLALSGDL-KTVTCSSENQHYPDSPERF-THSQVLSTQSFSSGRHYWEV--EVSESGGWSVGVAYPSIERKGD 79

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484 353 V------PK-----CPENGFWVVQLSKGTKYLSTFSaltpvmlmeppSHMGIFLDFEAGEVSFYSVSDG-SHLHTYSqAT 420
Cdd:cd12891   80 EsrigrnDKswcleWQDKSFSAWHNNEETPLPSVSS-----------RRLGVYLDYEAGRLSFYELSDPiRHLHTFT-AT 147


                 ....*....
gi 767912484 421 FPGPLQPFF 429
Cdd:cd12891  148 FTEPLHPAF 156

Bbox2_TRIM7-like

cd19762

B-box-type 2 zinc finger found in tripartite motif-containing proteins TRIM7, TRIM27 and ...

71-113

1.77e-19

Pssm-ID: 380820 [Multi-domain] Cd Length: 44 Bit Score: 81.20 E-value: 1.77e-19

                         10        20        30        40
                 ....*....|....*....|....*....|....*....|...
gi 767912484  71 LCQEHHEPLKLFCQKDQSPICVVCRESREHRLHRVLPAEEAVQ 113
Cdd:cd19762    2 VCEKHQEPLKLFCKEDKRPICVVCDRSREHRHHTVLPVEEAAQ 44

SPRY_PRY_TRIM10

cd15827

PRY/SPRY domain of tripartite motif-binding protein 10 (TRIM10) also known as hematopoietic ...

273-431

1.80e-19

PRY/SPRY domain of tripartite motif-binding protein 10 (TRIM10) also known as hematopoietic RING finger 1 (HERF1); This domain, consisting of the distinct N-terminal PRY subdomain followed by the SPRY subdomain, is found at the C-terminus of TRIM10, also known as RING finger protein 9 (RNF9) or hematopoietic RING finger 1 (HERF1). TRIM10 domain is composed of RING/B-box/coiled-coil core and also known as RBCC protein. TRIM10/HERF1 is predominantly expressed during definitive erythropoiesis and in embryonic liver, and minimally expressed in adult liver, kidney, and colon. It is critical for erythroid cell differentiation and its down-regulation leads to cell death; inhibition of TRIM10 expression blocks terminal erythroid differentiation, while its over-expression in erythroid cells induces beta-major globin expression and erythroid differentiation.

Pssm-ID: 293999 [Multi-domain] Cd Length: 172 Bit Score: 85.27 E-value: 1.80e-19

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484 273 DATSAYPYLLLYESRQRRYLG----SSPEgsgfcSKDRFVAYPCAVGQTAFSSGRHYWEVGMNITGDALWALGVCRDNVS 348
Cdd:cd15827    7 DPQTSHPKLLLSEDHQRARFSykwqNSPD-----NPQRFDRATCVLAHDGFTGGRHTWVVSVDLAHGGSCTVGVVSEDVR 81

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484 349 RKDRVPKCPENGFWVVQLSKGtkYLSTFSAL-TPVMLMEPPSHMGIFLDFEAGEVSFYSVSDGSHLHTYsQATFPGPLQP 427
Cdd:cd15827   82 RKGELRLRPEEGVWAVRLAWG--FVSALGSFpTRLALEEQPRQVRVSLDYEVGWVTFVNAVTQEPIYTF-TASFTQKVFP 158


                 ....
gi 767912484 428 FFCL 431
Cdd:cd15827  159 FFGL 162

SPRY_PRY_TRIM41

cd13741

PRY/SPRY domain in tripartite motif-binding protein 41 (TRIM41); This domain, consisting of ...

269-429

5.97e-19

PRY/SPRY domain in tripartite motif-binding protein 41 (TRIM41); This domain, consisting of the distinct N-terminal PRY subdomain followed by the SPRY subdomain, is found at the C-terminus of tripartite motif-containing protein 41 (TRIM41). TRIM41 (also known as RING finger-interacting protein with C kinase or RINCK) is localized to speckles in the cytoplasm and nucleus, and functions as an E3 ligase that catalyzes the ubiquitin-mediated degradation of protein kinase C.

Pssm-ID: 240499 [Multi-domain] Cd Length: 199 Bit Score: 84.81 E-value: 5.97e-19

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484 269 DVVPDATSAYPYLLLYESRQRRYLGSSPEGSGFCSKdRFVAYPCAVGQTAFSSGRHYWEVgmNITGDALWALGVCRDNVS 348
Cdd:cd13741    1 DLTLDPDTAHPALLLSPDRRGVRLAERRQEVPEHPK-RFSADCCVLGAQGFRSGRHYWEV--EVGGRRGWAVGAARESTH 77

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484 349 RKDRV--------------------------PKCP--ENGFWVVQlSKGTKYLSTFSalTPVMLMEP---PSHMGIFLDF 397
Cdd:cd13741   78 HKEKVgsggssvssgdasssrhhhrrrrlhlPQQPllQREVWCVG-TNGKRYQAQSS--TEQTLLSPsekPRRFGVYLDY 154

                        170       180       190
                 ....*....|....*....|....*....|..
gi 767912484 398 EAGEVSFYSVSDGSHLHTYSQATFPGPLQPFF 429
Cdd:cd13741  155 EAGRLGFYNAETLAHVHTFSAAFLGERVFPFF 186

SPRY_PRY_TRIM22

cd15824

PRY/SPRY domain in tripartite motif-containing protein 22 (TRIM22), also known as RING finger ...

310-429

2.10e-18

PRY/SPRY domain in tripartite motif-containing protein 22 (TRIM22), also known as RING finger protein 94 (RNF94) or Stimulated trans-acting factor of 50 kDa (STAF50); This domain, consisting of the distinct N-terminal PRY subdomain followed by the SPRY subdomain, is found at the C-terminus of TRIM22, also known as RING finger protein 94 (RNF94) or STAF50 (Stimulated trans-acting factor of 50 kDa). TRIM6 domain is composed of RING/B-box/coiled-coil core and also known as RBCC protein. TRIM22 is an interferon-induced protein, predominantly expressed in peripheral blood leukocytes, in lymphoid tissue such as spleen and thymus, and in the ovary.TRIM22 plays an integral role in the host innate immune response to viruses; it has been shown to inhibit the replication of a number of viruses, including HIV-1, hepatitis B, and influenza A. TRIM22 inhibits influenza A virus (IAV) infection by targeting the viral nucleoprotein for degradation; it represents a novel restriction factor up-regulated upon IAV infection that curtails its replicative capacity in epithelial cells. Altered TRIM22 expression has also been associated with multiple sclerosis, cancer, and autoimmune disease. A large number of high-risk non-synonymous (ns)SNPs have been identified in the highly polymorphic TRIM22 gene, most of which are located in the SPRY domain and could possibly alter critical regions of the SPRY structural and functional residues, including several sites that undergo post-translational modification. TRIM22 is a direct p53 target gene and inhibits the clonogenic growth of leukemic cells. Its expression in Wilms tumors is negatively associated with disease relapse. It is greatly under-expressed in breast cancer cells as compared to non-malignant cell lines; p53 dysfunction may be one of the mechanisms for its down-regulation.

Pssm-ID: 293996 [Multi-domain] Cd Length: 198 Bit Score: 82.97 E-value: 2.10e-18

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484 310 YPC------AVGQTAFSSGRHYWEVgmNITGDALWALGVC--RDNVSRKDRV----------PKC-----PENGFWVVQL 366
Cdd:cd15824   37 NPCdfsafdVLGCQYFSSGKYYWEV--DVSGKIAWILGVYskRNNLNKRKSSgfafdpnvnhPNVysryrPQNGYWVIGL 114

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|.
gi 767912484 367 SKGTKY-------LSTFSALTPVMLMePPSHMGIFLDFEAGEVSFYSVSD-GSHLHTYSQATFPGPLQPFF 429
Cdd:cd15824  115 QNESEYnafedssSSDPKVLTLSMAV-PPHRVGVFLDYEAGTVSFFNVTNhGSLIYKFSKCCFSQPVYPYF 184

SPRY_PRY_TRIM62

cd13744

PRY/SPRY domain in tripartite motif-binding protein 62 (TRIM62); This domain, consisting of ...

303-432

4.92e-18

PRY/SPRY domain in tripartite motif-binding protein 62 (TRIM62); This domain, consisting of the distinct N-terminal PRY subdomain followed by the SPRY subdomain, is found at the C-terminus of TRIM62. It is also called DEAR1 ductal epithelium (associated RING chromosome 1) and is involved in the morphogenesis of the mammary gland; loss of TRIM62 gene expression in breast is associated with increased risk of recurrence in early-onset breast cancer and thus, making TRIM62 a predictive biomarker. Non-small cell lung cancer lesions show a step-wise loss of TRIM62 levels during disease progression, indicating that it may play a role in the evolution of lung cancer. Decreased levels of TRIM62 also represent an independent adverse prognostic factor in AML.

Pssm-ID: 293978 Cd Length: 188 Bit Score: 81.97 E-value: 4.92e-18

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484 303 SKDRFVAYPCAVGQTAFSSGRHYWEVgmNITGDALWALGVCRDNVSRKDRVPKCPENGFWVVQLSKGTKYLSTFSALTPV 382
Cdd:cd13744   47 SPKRFDVEVSVLGSEGFSGGVHYWEV--VVSEKTQWMIGLAHEAVSRKGSIQIQPGRGFYCIVMHDGNQYSACTEPWTRL 124

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|
gi 767912484 383 MLMEPPSHMGIFLDFEAGEVSFYSVSDGSHLHTYSQaTFPGPLQPFFCLG 432
Cdd:cd13744  125 NVKSKLEKVGVYLDYDKGLLIFYNADDMSWLYTFRE-KFPGKLCSYFSPG 173

Bbox2_TRIM4-like

cd19760

B-box-type 2 zinc finger found in tripartite motif-containing proteins, TRIM4, TRIM17, TRIM41, ...

70-107

4.93e-18

B-box-type 2 zinc finger found in tripartite motif-containing proteins, TRIM4, TRIM17, TRIM41, TRIM52 and similar proteins; This family includes a group of tripartite motif-containing proteins, including TRIM4, TRIM17, TRIM41 and TRIM52. TRIM4, also known as RING finger protein 87 (RNF87), is a cytoplasmic E3 ubiquitin-protein ligase that recently evolved and is present only in higher mammals. It transiently interacts with mitochondria, induces mitochondrial aggregation and sensitizes the cells to hydrogen peroxide (H2O2) induced death. Its interaction with peroxiredoxin 1 (PRX1) is critical for the regulation of H2O2 induced cell death. Moreover, TRIM4 functions as a positive regulator of RIG-I-mediated type I interferon induction. It regulates the K63-linked ubiquitination of RIG-1 and assembly of antiviral signaling complex at mitochondria. TRIM17, also known as RING finger protein 16 (RNF16) or testis RING finger protein (Terf), is a crucial E3 ubiquitin ligase that is necessary and sufficient for neuronal apoptosis and contributes to Mcl-1 ubiquitination in cerebellar granule neurons (CGNs). It interacts in a SUMO-dependent manner with nuclear factor of activated T cell NFATc3 transcription factor, and thus inhibits the activity of NFATc3 by preventing its nuclear localization. In contrast, it binds to and inhibits NFATc4 transcription factor in a SUMO-independent manner. Moreover, TRIM17 stimulates degradation of kinetochore protein ZW10 interacting protein (ZWINT), a known component of the kinetochore complex required for mitotic spindle checkpoint, and negatively regulates cell proliferation. TRIM41, also known as RING finger-interacting protein with C kinase (RINCK), is an E3 ubiquitin-protein ligase that promotes the ubiquitination of protein kinase C (PKC) isozymes in cells. It specifically recognizes the C1 domain of PKC isozymes. It controls the amplitude of PKC signaling by controlling the amount of PKC in the cell. TRIM52, also known as RING finger protein 102 (RNF102), is encoded by a novel, noncanonical antiviral TRIM52 gene in primate genomes with unique specificity determined by the rapidly evolving RING domain. TRIM4, TRIM17 and TRIM41 belong to the C-IV subclass of TRIM (tripartite motif) family of proteins that are defined by their N-terminal RBCC (RING, Bbox, and coiled coil) domains, including three consecutive zinc-binding domains, a RING finger, Bbox2, and a coiled coil region, as well as a B30.2/SPRY (SplA and ryanodine receptor) domain positioned C-terminal to the RBCC domain. In contrast, TRIM52 lacks the putative viral recognition SPRY/B30.2 domain, and thus has been classified to the C-V subclass of TRIM family that contains only RBCC domains. The type 2 B-box (Bbox2) zinc finger is characterized by a CHC3H2 zinc-binding consensus motif.

Pssm-ID: 380818 [Multi-domain] Cd Length: 39 Bit Score: 77.29 E-value: 4.93e-18

                         10        20        30
                 ....*....|....*....|....*....|....*...
gi 767912484  70 DLCQEHHEPLKLFCQKDQSPICVVCRESREHRLHRVLP 107
Cdd:cd19760    1 GLCEKHQEPLKLFCEEDEALICVICRESRAHRAHTVVP 38

Bbox2_TRIM11_C-IV

cd19766

B-box-type 2 zinc finger found in tripartite motif-containing protein 11 (TRIM11) and similar ...

71-113

1.16e-16

B-box-type 2 zinc finger found in tripartite motif-containing protein 11 (TRIM11) and similar proteins; TRIM11, also known as protein BIA1, or RING finger protein 92 (RNF92), is an E3 ubiquitin-protein ligase involved in the development of the central nervous system. It is overexpressed in high-grade gliomas and promotes proliferation, invasion, migration and glial tumor growth. TRIM11 acts as a potential therapeutic target for congenital central hypoventilation syndrome (CCHS) through mediating the degradation of CCHS-associated polyalanine-expanded Phox2b. Trim11 modulates the function of neurogenic transcription factor Pax6 through the ubiquitin-proteosome system, and thus plays an essential role for Pax6-dependent neurogenesis. It also binds to and destabilizes a key component of the activator-mediated cofactor complex (ARC105), humanin, a neuroprotective peptide against Alzheimer's disease-relevant insults, and further regulates ARC105 function in transforming growth factor beta (TGFbeta) signaling. Moreover, TRIM11 negatively regulates retinoic acid-inducible gene-I (RIG-I)-mediated interferon-beta (IFNbeta) production and antiviral activity by targeting TANK-binding kinase-1 (TBK1). It may contribute to the endogenous restriction of retroviruses in cells. It enhances N-tropic murine leukemia virus (N-MLV) entry by interfering with Ref1 restriction. It also suppresses the early steps of human immunodeficiency virus HIV-1 transduction, resulting in decreased reverse transcripts. TRIM11 belongs to the C-IV subclass of TRIM (tripartite motif) family of proteins that are defined by their N-terminal RBCC (RING, Bbox, and coiled coil) domains, including three consecutive zinc-binding domains, a RING finger, Bbox2, and a coiled coil region, as well as a SPRY/B30.2 domain positioned C-terminal to the RBCC domain. The type 2 B-box (Bbox2) zinc finger is characterized by a CHC3H2 zinc-binding consensus motif.

Pssm-ID: 380824 [Multi-domain] Cd Length: 44 Bit Score: 73.32 E-value: 1.16e-16

                         10        20        30        40
                 ....*....|....*....|....*....|....*....|...
gi 767912484  71 LCQEHHEPLKLFCQKDQSPICVVCRESREHRLHRVLPAEEAVQ 113
Cdd:cd19766    2 LCGKHREPLKLFCKDHEALLCVVCERSREHWGHRVVPAEEAAQ 44

SPRY_PRY_TRIM14

cd13738

PRY/SPRY domain of tripartite motif-binding protein 14 (TRIM14); This is a TRIM14 domain ...

273-431

2.17e-16

PRY/SPRY domain of tripartite motif-binding protein 14 (TRIM14); This is a TRIM14 domain family contains residues in the N-terminus that form a distinct PRY domain structure such that the B30.2 domain consists of PRY and SPRY subdomains. TRIM14 domains have yet to be characterized. These B30.2 domains are a more recent adaptation where the SPRY/PRY combination is a possible component of immune defense. It belongs to Class IV TRIM protein family which has members involved in antiviral immunity at various levels of interferon signaling cascade.

Pssm-ID: 293973 [Multi-domain] Cd Length: 173 Bit Score: 76.75 E-value: 2.17e-16

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484 273 DATSAYPYLLLYESR---QRRYLGSSpegsGFCSKDRFVAYPCAVGQTAFSSGRHYWEVGMNITGdALWALGVCRDNVSR 349
Cdd:cd13738    4 EPDTLHPRLRLSDDRltvSCGWLGTL----GLCPPQRFDKLWQVLSRDSFFSGRHYWEVDLQEAG-AGWWVGAAYPSIGR 78

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484 350 K----------DRVPKCPEN---GFWVVQlsKGTKylstfsalTPVMLMEPPSHMGIFLDFEAGEVSFYSVSDG-SHLHT 415
Cdd:cd13738   79 KgdseaarlgwNRQSWCLKRydlEYWAFH--DGQR--------SRLRPEDDPDRLGVFLDYEAGILSFYDVTGGmTHLHT 148

                        170
                 ....*....|....*.
gi 767912484 416 YsQATFPGPLQPFFCL 431
Cdd:cd13738  149 F-RATFQEPLYPALRL 163

Bbox2_xNF7-like

cd19800

B-box-type 2 zinc finger found in Xenopus laevis nuclear factor 7 (xNF7) and similar proteins; ...

70-107

1.60e-14

B-box-type 2 zinc finger found in Xenopus laevis nuclear factor 7 (xNF7) and similar proteins; xNF7 is a maternally expressed novel zinc finger nuclear phosphoprotein. It acts as a transcription factor that determines dorsal-ventral body axis. xNF7 harbors a B-box motif that shows high sequence similarity with B-Box-type zinc finger 2 found in tripartite motif-containing proteins (TRIMs). The type 2 B-box (Bbox2) zinc finger is characterized by a CHC3H2 zinc-binding consensus motif.

Pssm-ID: 380858 [Multi-domain] Cd Length: 39 Bit Score: 67.04 E-value: 1.60e-14

                         10        20        30
                 ....*....|....*....|....*....|....*...
gi 767912484  70 DLCQEHHEPLKLFCQKDQSPICVVCRESREHRLHRVLP 107
Cdd:cd19800    1 EVCSEHDEPLKLFCKDDKRLICVICRDSRKHRGHRFLP 38

SPRY_PRY_SPRYD4

cd12903

PRY/SPRY domain containing protein 4 (SPRYD4); This domain, consisting of the distinct ...

305-431

2.15e-14

PRY/SPRY domain containing protein 4 (SPRYD4); This domain, consisting of the distinct N-terminal PRY subdomain followed by the SPRY subdomain and is encoded by the SPRYD4 gene. SPRYD4 (SPRY containing domain 4) is ubiquitously expressed in many human tissues, most strongly in kidney, bladder, brain, thymus and stomach. Subcellular localization demonstrates that SPRYD4 protein is localized in the nucleus when overexpressed in COS-7 green monkey cell. It has remained uncharacterized thus far.

Pssm-ID: 293960 Cd Length: 169 Bit Score: 70.94 E-value: 2.15e-14

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484 305 DRFVAYPCAVGQTAFSSGRHYWEVGMNITGDalWALGVCRDNVSRKDRVPKcpENGFWVVQLSKGTKYLSTFSALTPVML 384
Cdd:cd12903   38 ERFRDWAVVLGDTPVTSGRHYWEVTVKRSQE--FRIGVADVDMSRDECIGT--NESSWVFAYAQRKWYAMVANETVPVPL 113

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|....*..
gi 767912484 385 MEPPSHMGIFLDFEAGEVSFYSVSDGSHLHTYSqATFPGPLQPFFCL 431
Cdd:cd12903  114 VGKPDRVGLLLDYEAGKLSLVDVEKNSVVHTMS-AEFRGPVVPAFAL 159

SPRY_BSPRY

cd12904

SPRY domain in Ro-Ret family; This domain, named BSPRY, has been identified in the Ro-Ret ...

273-429

4.36e-14

SPRY domain in Ro-Ret family; This domain, named BSPRY, has been identified in the Ro-Ret family, since the protein is composed of a B-box, an alpha-helical coiled coil and a SPRY domain. The gene for BSPRY resides on human chromosome 9 and is specifically expressed in testis. The function of BSPRY is not known, but several related proteins of the RING-Box-coiled-coil (RBCC) family have been implicated in cell transformation.

Pssm-ID: 293961 [Multi-domain] Cd Length: 171 Bit Score: 70.14 E-value: 4.36e-14

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484 273 DATSAYPYLLLyeSRQRRYLGSSPEGSGFCSKD---RFVAYPCAVGQTAFSSGRHYWEVgmNITGDALWALGVCRDNVSR 349
Cdd:cd12904    4 DERTVSPLLSL--SEDRRTLTFSPKKARQSPPDdpeRFDHWPNALASLSFSSGTHAWVV--DVGKSCAYKVGVCYGSLER 79

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484 350 KDRVPKCP--ENGF-WVvqLSKGTKYLSTFSA--LTPVMLMEPPSHMGIFLDFEAGEVSFYSVSDGSHLHTYsQATFPGP 424
Cdd:cd12904   80 KGSGNEARlgYNAFsWV--FSRYDGEFSFSHNgqHVPLELLKCPARVGVLLDWPSQELLFYDPDSCTVLHSH-REAFAAP 156


                 ....*
gi 767912484 425 LQPFF 429
Cdd:cd12904  157 LLPVF 161

SPRY_PRY_RNF135

cd12902

PRY/SPRY domain in RING finger protein RNF135; This domain, consisting of the distinct ...

280-431

1.97e-13

PRY/SPRY domain in RING finger protein RNF135; This domain, consisting of the distinct N-terminal PRY subdomain followed by the SPRY subdomain, is found at the C-terminus of the RING finger protein RNF135 (also known as Riplet/RNF135), which ubiquitinates RIG-I (retinoic acid-inducible gene-I) to promote interferon-beta induction during the early phase of viral infection. Normally, RIG-I is activated by TRIM25 in response to viral infection, leading to activation of the RIG-I signaling pathway, thus resulting in type I interferon production to limit viral replication. However, RNF135, consisting of an N-terminal RING finger domain, C-terminal SPRY and PRY motifs and showing sequence similarity to TRIM25, acts as an alternative factor that promotes RIG-I activation independent of TRIM25.

Pssm-ID: 293959 [Multi-domain] Cd Length: 168 Bit Score: 67.93 E-value: 1.97e-13

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484 280 YLLLYESRQRRYLGSSPEGSGfCSKDRFVAYPCAVGQtAFSSGRHYWEVGMNITGDalWALGVCRDNVSRKDRVPKCPEN 359
Cdd:cd12902   11 SLEVSEDSRKVTVSHGPQAYA-WSPDRFSISQVLCSQ-AFSSGQHYWEVDTRQCSH--WAVGVASWEMSRDQMLGRTMDS 86

                         90       100       110       120       130       140       150
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|...
gi 767912484 360 gfWVVQLsKGTKYLSTFSALTPVML-MEPPSHMGIFLDFEAGEVSFYSVSDGSHLHTYSQATFPGPLQPFFCL 431
Cdd:cd12902   87 --WCIEW-KGTGQLSAWHMNKETVLgSDKPRVVGIWLDLEEGKLAFYSVANQERLLHECEVSASSPLHPAFWL 156

Bbox2_TRIM68_C-IV

cd19795

B-box-type 2 zinc finger found in tripartite motif-containing protein 68 (TRIM68) and similar ...

69-112

2.94e-12

B-box-type 2 zinc finger found in tripartite motif-containing protein 68 (TRIM68) and similar proteins; TRIM68, also known as RING finger protein 137 (RNF137) or SSA protein SS-56 (SS-56), is an E3 ubiquitin-protein ligase that negatively regulates Toll-like receptor (TLR)- and RIG-I-like receptor (RLR)-driven type I interferon production by degrading TRK fused gene (TFG), a novel driver of IFN-beta downstream of anti-viral detection systems. It also functions as a cofactor for androgen receptor-mediated transcription by regulating ligand-dependent transcription of androgen receptor in prostate cancer cells. Moreover, TRIM68 is a cellular target of autoantibody responses in Sjogren's syndrome (SS), as well as systemic lupus erythematosus (SLE). It is also an auto-antigen for T cells in SS and SLE. TRIM68 belongs the C-IV subclass of TRIM (tripartite motif) family of proteins that are defined by their N-terminal RBCC (RING, Bbox, and coiled coil) domains, including three consecutive zinc-binding domains, a RING finger, Bbox2, and two coiled coil domains, as well as a B30.2/SPRY (SplA and ryanodine receptor) domain positioned C-terminal to the RBCC domain. The type 2 B-box (Bbox2) zinc finger is characterized by a CHC3H2 zinc-binding consensus motif.

Pssm-ID: 380853 [Multi-domain] Cd Length: 44 Bit Score: 60.92 E-value: 2.94e-12

                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....
gi 767912484  69 QDLCQEHHEPLKLFCQKDQSPICVVCRESREHRLHRVLPAEEAV 112
Cdd:cd19795    1 EDLCERHKEKLNLFCEEDQELLCVVCEQSPEHKAHTVVPVEEAA 44

RING-HC_TRIM7-like_C-IV

cd16594

RING finger, HC subclass, found in tripartite motif-containing proteins, TRIM7, TRIM11 and ...

1-51

7.49e-12

RING finger, HC subclass, found in tripartite motif-containing proteins, TRIM7, TRIM11 and TRIM27, and similar proteins; TRIM7, TRIM11 and TRIM27, closely related tripartite motif-containing proteins, belong to the C-IV subclass of the TRIM (tripartite motif) family of proteins that are defined by their N-terminal RBCC (RING, Bbox, and coiled coil) domains, including three consecutive zinc-binding domains, a C3HC4-type RING-HC finger, Bbox2, and a coiled coil region, as well as a SPRY/B30.2 domain positioned C-terminal to the RBCC domain. TRIM7, also known as glycogenin-interacting protein (GNIP) or RING finger protein 90 (RNF90), is an E3 ubiquitin-protein ligase that mediates c-Jun/AP-1 activation by Ras signalling. Its phosphorylation and activation by MSK1 in response to direct activation by the Ras-Raf-MEK-ERK pathway can stimulate TRIM7 E3 ubiquitin ligase activity in mediating Lys63-linked ubiquitination of the AP-1 coactivator RACO-1, leading to RACO-1 protein stabilization. Moreover, TRIM7 binds and activates glycogenin, the self-glucosylating initiator of glycogen biosynthesis. TRIM11, also known as protein BIA1, or RING finger protein 92 (RNF92), is an E3 ubiquitin-protein ligase involved in the development of the central nervous system. It is overexpressed in high-grade gliomas and promotes proliferation, invasion, migration and glial tumor growth. TRIM11 acts as a potential therapeutic target for congenital central hypoventilation syndrome (CCHS) by mediating the degradation of CCHS-associated polyalanine-expanded Phox2b. TRIM11 modulates the function of neurogenic transcription factor Pax6 through the ubiquitin-proteosome system, and thus plays an essential role for Pax6-dependent neurogenesis. It also binds to and destabilizes a key component of the activator-mediated cofactor complex (ARC105), humanin, a neuroprotective peptide against Alzheimer's disease-relevant insults, and further regulates ARC105 function in transforming growth factor beta (TGFbeta) signaling. Moreover, TRIM11 negatively regulates retinoic acid-inducible gene-I (RIG-I)-mediated interferon-beta (IFNbeta) production and antiviral activity by targeting TANK-binding kinase-1 (TBK1). It may contribute to the endogenous restriction of retroviruses in cells. It enhances N-tropic murine leukemia virus (N-MLV) entry by interfering with Ref1 restriction. It also suppresses the early steps of human immunodeficiency virus HIV-1 transduction, resulting in decreased reverse transcripts. TRIM27, also known as RING finger protein 76 (RNF76), RET finger protein (RFP), or zinc finger protein RFP, is a nuclear E3 ubiquitin-protein ligase that is highly expressed in testis and in various tumor cell lines. Expression of TRIM27 is associated with prognosis of colon and endometrial cancers. TRIM27 was first identified as a fusion partner of the RET receptor tyrosine kinase. It functions as a transcriptional repressor and associates with several proteins involved in transcriptional activity, such as enhancer of polycomb 1 (Epc1), a member of the Polycomb group proteins, and Mi-2beta, a main component of the nucleosome remodeling and deacetylase (NuRD) complex, and the cell cycle regulator retinoblastoma protein (RB1). It also interacts with HDAC1, leading to downregulation of thioredoxin binding protein 2 (TBP-2), which inhibits the function of thioredoxin. Moreover, TRIM27 mediates Pax7-induced ubiquitination of MyoD in skeletal muscle atrophy. In addition, it inhibits muscle differentiation by modulating serum response factor (SRF) and Epc1. TRIM27 promotes a non-canonical polyubiquitination of PTEN, a lipid phosphatase that catalyzes PtdIns(3,4,5)P3 (PIP3) to PtdIns(4,5)P2 (PIP2). It is an IKKepsilon-interacting protein that regulates IkappaB kinase (IKK) function and negatively regulates signaling involved in the antiviral response and inflammation. TRIM27 also forms a protein complex with MBD4 or MBD2 or MBD3, and thus plays an important role in the enhancement of transcriptional repression through MBD proteins in tumorigenesis, spermatogenesis, and embryogenesis. It is a component of an estrogen receptor 1 (ESR1) regulatory complex that is involved in estrogen receptor-mediated transcription in MCF-7 cells.

Pssm-ID: 438256 [Multi-domain] Cd Length: 61 Bit Score: 60.39 E-value: 7.49e-12

                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|.
gi 767912484   1 MTTCGHNFCRACIQLSWEKARGkkgrrkrkgSFPCPECREMSPQRNLLPNR 51
Cdd:cd16594   20 TLDCGHSFCRACIARCWEEPET---------SASCPQCRETCPQRNLRPNR 61

SPRY_PRY_SNTX

cd16040

Stonustoxin subunit alpha or SNTX subunit alpha; This domain, consisting of the distinct ...

305-432

1.06e-11

Stonustoxin subunit alpha or SNTX subunit alpha; This domain, consisting of the distinct N-terminal PRY subdomain followed by the SPRY subdomain, is found at the C-terminus of Stonustoxin alpha proteins. Stonustoxin (SNTX) is a multifunctional lethal protein isolated from venom elaborated by the stonefish. It comprises two subunits, termed alpha and beta. SNTX elicits an array of biological responses, particularly a potent hypotension and respiratory difficulties.

Pssm-ID: 294002 [Multi-domain] Cd Length: 180 Bit Score: 63.27 E-value: 1.06e-11

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484 305 DRFVAYP---CAVGQTafssGRHYWEVGMNITGdalWALGVCRDNVSRKDRVPKCpenGF------WVVQLSKGtKYlsT 375
Cdd:cd16040   45 ERFDYWPqvlCREGLS----GRCYWEVEWSGGG---VDIAVAYKGISRKGDGDDS---RFgyndksWSLECSPS-GY--S 111

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*.
gi 767912484 376 F-----SALTPVmlmePPSHM---GIFLDFEAGEVSFYSVSDG-SHLHTYsQATFPGPLQPFFCLG 432
Cdd:cd16040  112 FwhnnkKTEISV----PSSSSsrvGVYLDHSAGTLSFYSVSDTmTLLHTV-QTTFTEPLYPGFGVG 172

Bbox2_TRIM39-like

cd19780

B-box-type 2 zinc finger found in tripartite motif-containing proteins TRIM39, TRIM58 and ...

71-111

1.96e-11

B-box-type 2 zinc finger found in tripartite motif-containing proteins TRIM39, TRIM58 and similar proteins; The family includes TRIM39 and TRIM58, both of which belong to the C-IV subclass of TRIM (tripartite motif) family of proteins that are defined by their N-terminal RBCC (RING, Bbox, and coiled coil) domains, including three consecutive zinc-binding domains, a RING finger, Bbox2, and a coiled coil region, as well as a SPRY/B30.2 domain positioned C-terminal to the RBCC domain. The type 2 B-box (Bbox2) zinc finger is characterized by a CHC3H2 zinc-binding consensus motif. TRIM39, also termed RING finger protein 23 (RNF23), or testis-abundant finger protein, is an E3 ubiquitin-protein ligase that plays a role in controlling DNA damage-induced apoptosis through inhibition of the anaphase promoting complex (APC/C), a multiprotein ubiquitin ligase that controls multiple cell cycle regulators, including cyclins, geminin, and others. TRIM39 also functions as a regulator of several key processes in the proliferative cycle. It directly regulates p53 stability and modulates cell cycle progression and DNA damage responses via stabilization of p21. TRIM39 also negatively regulates the nuclear factor kappaB (NFkappaB)-mediated signaling pathway through stabilization of Cactin, an inhibitor of NFkappaB- and Toll-like receptor (TLR)-mediated transcription, which is induced by inflammatory stimulants such as tumor necrosis factor alpha (TNFalpha). TRIM39 is a MOAP-1-binding protein that can promote apoptosis signaling through stabilization of MOAP-1 via the inhibition of its poly-ubiquitination process. TRIM58, also known as protein BIA2, is an erythroid E3 ubiquitin-protein ligase induced during late erythropoiesis. It binds and ubiquitinates the intermediate chain of the microtubule motor dynein (DYNC1LI1/DYNC1LI2), stimulating the degradation of the dynein holoprotein complex. It may participate in the erythroblast enucleation process through regulation of nuclear polarization.

Pssm-ID: 380838 [Multi-domain] Cd Length: 44 Bit Score: 58.62 E-value: 1.96e-11

                         10        20        30        40
                 ....*....|....*....|....*....|....*....|.
gi 767912484  71 LCQEHHEPLKLFCQKDQSPICVVCRESREHRLHRVLPAEEA 111
Cdd:cd19780    4 LCARHREALSLFCEEDQEAVCLVCEISHDHRAHTLVPLQDA 44

SPRY_PRY_TRIM18

cd12892

PRY/SPRY domain of TRIM18/MID1, also known as FXY or RNF59; This domain, consisting of the ...

303-429

2.14e-11

PRY/SPRY domain of TRIM18/MID1, also known as FXY or RNF59; This domain, consisting of the distinct N-terminal PRY subdomain followed by the SPRY subdomain, is at the C-terminus of the overall domain architecture of MID1 (also known as FXY, RNF59, TRIM18) gene represented by a RING finger domain (RING), two B-box motifs (BBOX), coiled-coil C-terminal to Bbox domain (BBC) and fibronectin type 3 domain (FN3). Mutations in the human MID1 gene result in X-linked Opitz G/BBB syndrome (OS), a disorder affecting development of midline structures, causing craniofacial, urogenital, gastrointestinal and cardiovascular abnormalities. A unique MID1 gene mutation located in a variable loop in the SPRY domain alters conformation of the binding pocket and may affect the binding affinity to the PRY/SPRY domain.

Pssm-ID: 240472 Cd Length: 177 Bit Score: 62.34 E-value: 2.14e-11

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484 303 SKDRFVAYPC--AVGQTAFSSGRHYWEVgmNITGDALWALGVCRDNVSRKDRVPKcpENGFWVVQLSKGTKYLSTFSALT 380
Cdd:cd12892   34 TPERFTSQGSygVAGNVFIDSGRHYWEV--VISGSTWYAIGIAYKSAPKHEWIGK--NSASWVLCRCNNNWVVRHNSKEI 109

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|..
gi 767912484 381 PVmlmEPPSHM---GIFLDFEAGEVSFYSVSDGSHLHTYSqATFPGPLQPFF 429
Cdd:cd12892  110 PI---EPSPHLrrvGILLDYDNGSLSFYDALNSIHLYTFD-IAFAQPVCPTF 157

Bbox2_TRIM16-like

cd19769

B-box-type 2 zinc finger found in tripartite motif-containing proteins, TRIM16, TRIM29, ...

71-116

1.93e-10

B-box-type 2 zinc finger found in tripartite motif-containing proteins, TRIM16, TRIM29, TRIM47 and similar proteins; This family includes a group of tripartite motif-containing proteins, such as TRIM16, TRIM29 and TRIM47. TRIM16, also termed estrogen-responsive B box protein (EBBP), is a regulator that may play a role in the regulation of keratinocyte differentiation. It may also act as a tumor suppressor through affecting cell proliferation and migration or tumorigenicity in carcinogenesis. TRIM29, also termed ataxia telangiectasia group D-associated protein (ATDC), plays a crucial role in the regulation of macrophage activation in response to viral or bacterial infections within the respiratory tract. TRIM47, also known as gene overexpressed in astrocytoma protein (GOA) or RING finger protein 100 (RNF100), plays an important role in the process of dedifferentiation that is associated with astrocytoma tumorigenesis. TRIM16 and TRIM29 belong to an unclassified TRIM (tripartite motif) family of proteins that do not have RING fingers and thus lack the characteristic tripartite (RING (R), B-box, and coiled coil (CC)) RBCC motif. TRIM47 belongs to the C-IV subclass of TRIM family of proteins that are defined by their N-terminal RBCC (RING, Bbox, and coiled coil) domains, including three consecutive zinc-binding domains, a RING finger, Bbox1 and Bbox2, and two coiled coil domains, as well as a B30.2/SPRY (SplA and ryanodine receptor) domain positioned C-terminal to the RBCC domain. The type 2 B-box (Bbox2) zinc finger is characterized by a CHC3H2 zinc-binding consensus motif.

Pssm-ID: 380827 [Multi-domain] Cd Length: 46 Bit Score: 55.79 E-value: 1.93e-10

                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....*.
gi 767912484  71 LCQEHHEPLKLFCQKDQSPICVVCRESrEHRLHRVLPAEEAVQGYK 116
Cdd:cd19769    2 VCPIHKKPLELFCRTDQMCICELCAKE-EHRGHDVVTVEEEREKKE 46

Bbox2_TRIM10-like

cd19765

B-box-type 2 zinc finger found in tripartite motif-containing proteins, TRIM10, TRIM15, ...

70-107

2.71e-10

B-box-type 2 zinc finger found in tripartite motif-containing proteins, TRIM10, TRIM15, TRIM26, TRIM31 and similar proteins; This family includes TRIM10, TRIM15, TRIM26 and TRIM31. TRIM10, also known as B30-RING finger protein (RFB30), RING finger protein 9 (RNF9), or hematopoietic RING finger 1 (HERF1), is a novel hematopoiesis-specific RING finger protein required for terminal differentiation of erythroid cells. TRIM15, also termed RING finger protein 93 (RNF93), or zinc finger protein 178 (ZNF178), or zinc finger protein B7 (ZNFB7), is a focal adhesion protein that regulates focal adhesion disassembly. It localizes to focal contacts in a myosin-II-independent manner by an interaction between its coiled-coil domain and the LD2 motif of paxillin. TRIM15 can also associate with coronin 1B, cortactin, filamin binding LIM protein1, and vasodilator-stimulated phosphoprotein, which are involved in actin cytoskeleton dynamics. As an additional component of the integrin adhesome, it regulates focal adhesion turnover and cell migration. TRIM26, also known as acid finger protein (AFP), RING finger protein 95 (RNF95), or zinc finger protein 173 (ZNF173), is an E3 ubiquitin-protein ligase that negatively regulates interferon-beta production and antiviral response through polyubiquitination and degradation of nuclear transcription factor IRF3. It functions as an important regulator for RNA virus-triggered innate immune response by bridging TBK1 to NEMO (NF-kappaB essential modulator, also known as IKKgamma) and mediating TBK1 activation. It also acts as a novel tumor suppressor of hepatocellular carcinoma by regulating cancer cell proliferation, colony forming ability, migration, and invasion. TRIM31 is an E3 ubiquitin-protein ligase that primarily localizes to the cytoplasm, but is also associated with the mitochondria. It can negatively regulate cell proliferation and may be a potential biomarker of gastric cancer as it is overexpressed from the early stage of gastric carcinogenesis. TRIM31 is downregulated in non-small cell lung cancer and serves as a potential tumor suppressor. It interacts with p52 (Shc) and inhibits Src-induced anchorage-independent growth. TRIM10, TRIM15 and TRIM26 belong to the C-IV subclass of TRIM (tripartite motif) family of proteins that are defined by their N-terminal RBCC (RING, Bbox, and coiled coil) domains, including three consecutive zinc-binding domains, a RING finger, Bbox2, and a coiled coil region, as well as a B30.2/SPRY (SplA and ryanodine receptor) domain positioned C-terminal to the RBCC domain. TRIM31 belongs to the C-V subclass of TRIM family of proteins. The type 2 B-box (Bbox2) zinc finger is characterized by a CHC3H2 zinc-binding consensus motif.

Pssm-ID: 380823 [Multi-domain] Cd Length: 39 Bit Score: 55.17 E-value: 2.71e-10

                         10        20        30
                 ....*....|....*....|....*....|....*...
gi 767912484  70 DLCQEHHEPLKLFCQKDQSPICVVCRESREHRLHRVLP 107
Cdd:cd19765    1 TLCEEHGEKIHFFCEDDGKFLCVVCRESREHRTHTVSL 38

Bbox2

cd19756

B-box-type 2 zinc finger (Bbox2); The B-box-type zinc finger is a short zinc binding domain of ...

71-107

6.99e-10

B-box-type 2 zinc finger (Bbox2); The B-box-type zinc finger is a short zinc binding domain of around 40 amino acid residues in length. It has been found in transcription factors, ribonucleoproteins and proto-oncoproteins, such as in TRIM (tripartite motif) proteins that consist of an N-terminal RING finger (originally called an A-box), followed by 1-2 B-box domains and a coiled-coil domain (also called RBCC for Ring, B-box, Coiled-Coil). The B-box-type zinc finger often presents in combination with other motifs, like RING zinc finger, NHL motif, coiled-coil or RFP domain in functionally unrelated proteins, most likely mediating protein-protein interaction. Based on different consensus sequence and the spacing of the 7-8 zinc-binding residues, B-box-type zinc fingers can be divided into two groups, type 1 (Bbox1: C6H2) and type 2 (Bbox2: CHC3H2). The family corresponds to type 2 B-box (Bbox2).

Pssm-ID: 380814 [Multi-domain] Cd Length: 39 Bit Score: 53.96 E-value: 6.99e-10

                         10        20        30
                 ....*....|....*....|....*....|....*...
gi 767912484  71 LCQEHHEP-LKLFCQKDQSPICVVCRESREHRLHRVLP 107
Cdd:cd19756    1 LCPEHPEEpLKLFCETCQELVCVLCLLSGEHRGHKVVP 38

PRY

smart00589

associated with SPRY domains;

267-319

7.89e-10

associated with SPRY domains;

Pssm-ID: 128857 Cd Length: 52 Bit Score: 54.12 E-value: 7.89e-10

                           10        20        30        40        50
                   ....*....|....*....|....*....|....*....|....*....|....
gi 767912484   267 LEDVVPDATSAYPYLLLYE-SRQRRYLGSSPegSGFCSKDRFVAYPCAVGQTAF 319
Cdd:smart00589   1 AVDVTLDPDTAHPYLLLSEdRRSVRYGDLKQ--SLPDNPERFDSYPCVLGSQGF 52

SPRY_PRY_FSD1

cd12901

Fibronectin type III and SPRY containing 1 (FSD1) domain includes PRY at the N-terminus; This ...

295-429

1.35e-09

Fibronectin type III and SPRY containing 1 (FSD1) domain includes PRY at the N-terminus; This domain is part of the fibronectin type III and SPRY domain containing 1 (FSD1) and FSD1-like (FSD1L) proteins. These are centrosome-associated proteins that are characterized by an N-terminal coiled-coil region downstream of B-box (BBC) domain, a central fibronectin type III (FN3) domain, and C-terminal repeats in PRY/SPRY domain. The FSD1 protein associates with a subset of microtubules and may be involved in the stability and organization of microtubules during cytokinesis.

Pssm-ID: 293958 Cd Length: 207 Bit Score: 57.91 E-value: 1.35e-09

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484 295 SPEGSGfcSKDRFVAYPCAV-GQTAFSSGRHYWEVGMNITGDAlWALGVCRDNVSRKDRVPKcpENGFWVVQLSKGTKyl 373
Cdd:cd12901   59 MPSARG--GRDRFTAESYTVlGDTLIDGGQHYWEVRAQKDSKA-FSVGVAYRSLGKFDQLGK--TNASWCLHVNNWLQ-- 131

                         90       100       110       120       130       140
                 ....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484 374 STFSAL----TPVMLMEPPSHMGIFLDFEAGEVSFYSVSDGSHLHTYsQATFPGPLQPFF 429
Cdd:cd12901  132 NSFAAKhnnkAKTLDVPVPDRIGVYCDFDEGQLSFYNARTKQLLHTF-KMKFTQPVLPAF 190

SPRY_PRY_TRIM65

cd12896

PRY/SPRY domain in tripartite motif-containing domain 65 (TRIM65); This domain, consisting of ...

273-432

2.22e-09

PRY/SPRY domain in tripartite motif-containing domain 65 (TRIM65); This domain, consisting of the distinct N-terminal PRY subdomain followed by the SPRY subdomain, is found at the C-terminus of TRIM65 proteins (composed of RING/B-box/coiled-coil core and also known as RBCC proteins). The SPRY/PRY combination is a possible component of immune defense. This protein family has not been characterized.

Pssm-ID: 293953 [Multi-domain] Cd Length: 182 Bit Score: 56.69 E-value: 2.22e-09

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484 273 DATSAYPYLLLYESRQRRYLGSSPEGSGFCSKDRFVAYP--CAVGqtaFSSGRHYWEVGMNitgDALWALGVCRDNVSRK 350
Cdd:cd12896   15 DPRTANKYLELSRQNRRAKHGRSAARGVPASPGSFELWQvqCTQS---FQHGHHYWEVEVS---SHSVTLGVTYPGLPRH 88

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484 351 dRVPKCPEN-----GFWVVQLSKGTkyLSTFSALTPVMLMEPP-SHMGIFLDFEAGEVSFYSVSDG-SHLHTYSqATFPG 423
Cdd:cd12896   89 -KQGGHKDNigrnpCSWGLQIQEDS--LQAWHNGRAQKLQGVSyRLLGVDLDLEAGTLTFYGLEPGtQRLHTFH-AIFTQ 164


                 ....*....
gi 767912484 424 PLQPFFCLG 432
Cdd:cd12896  165 PLYPVFWLL 173

RING-HC_TRIM69_C-IV

cd16611

RING finger, HC subclass, found in tripartite motif-containing protein 69 (TRIM69) and similar ...

1-50

2.39e-09

RING finger, HC subclass, found in tripartite motif-containing protein 69 (TRIM69) and similar proteins; TRIM69, also known as RFP-like domain-containing protein trimless or RING finger protein 36 (RNF36), is a testis E3 ubiquitin-protein ligase that plays a specific role in apoptosis and may also play an important role in germ cell homeostasis during spermatogenesis. TRIM69 belongs to the C-IV subclass of the TRIM (tripartite motif) family of proteins that are defined by their N-terminal RBCC (RING, Bbox, and coiled coil) domains, including three consecutive zinc-binding domains, a C3HC4-type RING-HC finger, Bbox1 and Bbox2, and a coiled coil region, as well as a B30.2/SPRY (SplA and ryanodine receptor) domain positioned C-terminal to the RBCC domain.

Pssm-ID: 438273 [Multi-domain] Cd Length: 59 Bit Score: 53.22 E-value: 2.39e-09

                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|
gi 767912484   1 MTTCGHNFCRACIQLSWEKARGKKGrrkrkgsfpCPECREMSPQRNLLPN 50
Cdd:cd16611   19 MLSCGHNFCQSCITGFWELQAEDTT---------CPECRELCQYRNLTPN 59

Bbox2_TRIM35_C-IV

cd19777

B-box-type 2 zinc finger found in tripartite motif-containing protein 35 (TRIM35) and similar ...

71-110

3.12e-09

B-box-type 2 zinc finger found in tripartite motif-containing protein 35 (TRIM35) and similar proteins; TRIM35, also known as hemopoietic lineage switch protein 5 (HLS5), is a putative hepatocellular carcinoma (HCC) suppressor that inhibits phosphorylation of pyruvate kinase isoform M2 (PKM2), which is involved in aerobic glycolysis of cancer cells and further suppresses the Warburg effect and tumorigenicity in HCC. It also negatively regulates Toll-like receptor 7 (TLR7)- and TLR9-mediated type I interferon production by suppressing the stability of interferon regulatory factor 7 (IRF7). Moreover, TRIM35 regulates erythroid differentiation by modulating globin transcription factor 1 (GATA-1) activity. TRIM35 belongs to the C-IV subclass of TRIM (tripartite motif) family of proteins that are defined by their N-terminal RBCC (RING, Bbox, and coiled coil) domains, including three consecutive zinc-binding domains, a RING finger, Bbox2, and a coiled coil region, as well as a B30.2/SPRY (SplA and ryanodine receptor) domain positioned C-terminal to the RBCC domain. The type 2 B-box (Bbox2) zinc finger is characterized by a CHC3H2 zinc-binding consensus motif.

Pssm-ID: 380835 [Multi-domain] Cd Length: 44 Bit Score: 52.48 E-value: 3.12e-09

                         10        20        30        40
                 ....*....|....*....|....*....|....*....|
gi 767912484  71 LCQEHHEPLKLFCQKDQSPICVVCRESREHRLHRVLPAEE 110
Cdd:cd19777    5 LCRLHGETLKLFCLDDKELLCCACQSSKQHQGHRVYPVKE 44

Bbox2_TRIM40_C-V

cd19781

B-box-type 2 zinc finger found in tripartite motif-containing protein 40 (TRIM40) and similar ...

71-111

1.17e-08

B-box-type 2 zinc finger found in tripartite motif-containing protein 40 (TRIM40) and similar proteins; TRIM40, also termed probable E3 NEDD8-protein ligase, or RING finger protein 35, may function as an E3 ubiquitin-protein ligase of the NEDD8 conjugation pathway. It promotes neddylation of IKBKG/NEMO, stabilizing NFKBIA, and inhibiting NF-kappaB nuclear translocation and activity. TRIM40 belongs to the C-V subclass of TRIM (tripartite motif) family of proteins that are defined by an N-terminal RBCC (RING, Bbox, and coiled coil) domains, including three consecutive zinc-binding domains, a RING finger, Bbox2, and a coiled coil region. The type 2 B-box (Bbox2) zinc finger is characterized by a CHC3H2 zinc-binding consensus motif.

Pssm-ID: 380839 [Multi-domain] Cd Length: 44 Bit Score: 50.88 E-value: 1.17e-08

                         10        20        30        40
                 ....*....|....*....|....*....|....*....|.
gi 767912484  71 LCQEHHEPLKLFCQKDQSPICVVCRESREHRLHRVLPAEEA 111
Cdd:cd19781    4 LCQLHEKKVEWFCEEDQVLLCEECLKSPEHQSHHVLTIEDA 44

BBOX

smart00336

B-Box-type zinc finger;

67-107

2.02e-08

B-Box-type zinc finger;

Pssm-ID: 197662 [Multi-domain] Cd Length: 42 Bit Score: 50.03 E-value: 2.02e-08

                           10        20        30        40
                   ....*....|....*....|....*....|....*....|..
gi 767912484    67 QKQDLCQEHH-EPLKLFCQKDQSPICVVCRESrEHRLHRVLP 107
Cdd:smart00336   1 QRAPKCDSHGdEPAEFFCEECGALLCRTCDEA-EHRGHTVVL 41

SPRY_PRY_TRIM76_like

cd12899

PRY/SPRY domain in tripartite motif-containing protein 76 (TRIM76)-like; This domain is ...

322-434

2.33e-08

PRY/SPRY domain in tripartite motif-containing protein 76 (TRIM76)-like; This domain is similar to the distinct PRY/SPRY subdomain found at the C-terminus of TRIM76, a Class I TRIM protein. TRIM76 (also known as cardiomyopathy-associated protein 5 or CMYA5 or myospryn or SPRYD2) is a muscle-specific member of the TRIM superfamily, but lacks the RING domain. It has been suggested that TRIM76 is involved in two distinct processes, protein kinase A signaling and vesicular trafficking.

Pssm-ID: 293956 [Multi-domain] Cd Length: 176 Bit Score: 53.64 E-value: 2.33e-08

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484 322 GRHYWEVGMNITGDalWALGVCRDNVSRKDRVPKcpENGFWVVQ---LSKGTKYLSTFSALTP-VMLMEPPSHMGIFLDF 397
Cdd:cd12899   56 GKHYWEVEVDEQTE--YRVGVAFEDTQRNGYLGA--NNTSWCMRhiiTPSRHKYEFLHNGWTPdIRITVPPKKIGILLDY 131

                         90       100       110
                 ....*....|....*....|....*....|....*..
gi 767912484 398 EAGEVSFYSVSDGSHLHTYSqATFPGPLQPFFCLGAP 434
Cdd:cd12899  132 DSGRLSFFNVDLAQHLYTFS-CQFQHFVHPCFSLEKP 167

Bbox2_TRIM60-like

cd19791

B-box-type 2 zinc finger found in tripartite motif-containing proteins, TRIM60, TRIM61, ...

71-107

2.40e-08

B-box-type 2 zinc finger found in tripartite motif-containing proteins, TRIM60, TRIM61, TRIM75 and similar proteins; This family includes a group of tripartite motif-containing proteins, including TRIM60, TRIM61 and TRIM75. TRIM60, also known as RING finger protein 129 (RNF129) or RING finger protein 33 (RNF33), is a cytoplasmic protein expressed in the testis. It may play an important role in the spermatogenesis process, the development of the preimplantation embryo, and in testicular functions. TRIM60 interacts with the cytoplasmic kinesin motor proteins KIF3A and KIF3B suggesting possible contribution to cargo movement along the microtubule in the expressed sites. It is also involved in spermatogenesis in Sertoli cells under the regulation of nuclear factor-kappaB (NF-kappaB). TRIM61 is closely related to TRIM60, but its biological function remains unclear. TRIM75 could be the product of a pseudogene. Its biological function remains unclear. TRIM60 and TRIM75 belong to the C-IV subclass of the TRIM (tripartite motif) family of proteins that are defined by their N-terminal RBCC (RING, Bbox, and coiled coil) domains, including three consecutive zinc-binding domains, a RING finger, Bbox2, and two coiled coil domains, as well as a B30.2/SPRY (SplA and ryanodine receptor) domain positioned C-terminal to the RBCC domain. In contrast, TRIM61 belongs to the C-V subclass of TRIM family that contains RBCC domains only. The type 2 B-box (Bbox2) zinc finger is characterized by a CHC3H2 zinc-binding consensus motif.

Pssm-ID: 380849 [Multi-domain] Cd Length: 39 Bit Score: 49.84 E-value: 2.40e-08

                         10        20        30
                 ....*....|....*....|....*....|....*..
gi 767912484  71 LCQEHHEPLKLFCQKDQSPICVVCRESREHRLHRVLP 107
Cdd:cd19791    2 LCEKHNQPLTKFCKKDLEPLCPQCSQSTDHQHHVVVP 38

Bbox2_TRIM72_C-IV

cd19797

B-box-type 2 zinc finger found in tripartite motif-containing protein 72 (TRIM72) and similar ...

70-111

2.65e-08

B-box-type 2 zinc finger found in tripartite motif-containing protein 72 (TRIM72) and similar proteins; TRIM72, also known as Mitsugumin-53 (MG53), is a muscle-specific protein that plays a central role in cell membrane repair by nucleating the assembly of the repair machinery at muscle injury sites. It is required in repair of alveolar epithelial cells under plasma membrane stress failure. It interacts with dysferlin to regulate sarcolemmal repair. Upregulation of TRIM72 develops obesity, systemic insulin resistance, dyslipidemia, and hyperglycemia, as well as induces diabetic cardiomyopathy through transcriptional activation of peroxisome proliferation-activated receptor alpha (PPAR-alpha) signaling pathway. Compensation for the absence of AKT signaling by ERK signaling during TRIM72 overexpression leads to pathological hypertrophy. Moreover, TRIM72 functions as a novel negative feedback regulator of myogenesis via targeting insulin receptor substrate-1 (IRS-1). It is transcriptionally activated by the synergism of myogenin (MyoD) and myocyte enhancer factor 2 (MEF2). TRIM72 belongs to the C-IV subclass of TRIM (tripartite motif) family of proteins that are defined by their N-terminal RBCC (RING, Bbox, and coiled coil) domains, including three consecutive zinc-binding domains, a RING finger, Bbox1 and Bbox2, and a coiled coil region, as well as a B30.2/SPRY (SplA and ryanodine receptor) domain positioned C-terminal to the RBCC domain. The type 2 B-box (Bbox2) zinc finger is characterized by a CHC3H2 zinc-binding consensus motif.

Pssm-ID: 380855 [Multi-domain] Cd Length: 42 Bit Score: 49.59 E-value: 2.65e-08

                         10        20        30        40
                 ....*....|....*....|....*....|....*....|..
gi 767912484  70 DLCQEHHEPLKLFCQKDQSPICVVCRESREHRLHRVLPAEEA 111
Cdd:cd19797    1 GHCEEHLDPLSVYCEQDRALICGVCASLGKHKGHNIITAAEA 42

Bbox2_TRIM5-like

cd19761

B-box-type 2 zinc finger found in tripartite motif-containing proteins, TRIM5, TRIM6, TRIM22, ...

70-109

2.78e-08

B-box-type 2 zinc finger found in tripartite motif-containing proteins, TRIM5, TRIM6, TRIM22, TRIM34, TRIM38 and similar proteins; The family includes TRIM5, TRIM6, TRIM22, TRIM34, and TRIM38, all of which belong to the C-IV subclass of the TRIM (tripartite motif) family of proteins that are defined by their N-terminal RBCC (RING, Bbox, and coiled coil) domains, including three consecutive zinc-binding domains, a RING finger, Bbox2, and a coiled coil region, as well as a B30.2/SPRY (SplA and ryanodine receptor) domain positioned C-terminal to the RBCC domain. The type 2 B-box (Bbox2) zinc finger is characterized by a CHC3H2 zinc-binding consensus motif. TRIM5, also termed RING finger protein 88 (RNF88), is a capsid-specific restriction factor that prevents infection from non-host-adapted retroviruses in a species-specific manner by binding to and destabilizing the retroviral capsid lattice before reverse transcription is completed. Its retroviral restriction activity correlates with the ability to activate TAK1-dependent innate immune signaling. TRIM5 also acts as a pattern recognition receptor that activates innate immune signaling in response to the retroviral capsid lattice. Moreover, TRIM5 plays a role in regulating autophagy through activation of autophagy regulator BECN1 by causing its dissociation from its inhibitors BCL2 and TAB2. It also plays a role in autophagy by acting as a selective autophagy receptor which recognizes and targets HIV-1 capsid protein p24 for autophagic destruction. TRIM6, also termed RING finger protein 89 (RNF89), is an E3-ubiquitin ligase that cooperates with the E2-ubiquitin conjugase UbE2K to catalyze the synthesis of unanchored K48-linked polyubiquitin chains, and further stimulates the interferon-I kappa B kinase epsilon (IKKepsilon) kinase-mediated antiviral response. It also regulates the transcriptional activity of Myc during the maintenance of embryonic stem (ES) cell pluripotency, and may act as a novel regulator for Myc-mediated transcription in ES cells. TRIM22, also termed 50 kDa-stimulated trans-acting factor (Staf-50), or RING finger protein 94 (RNF94), is an E3 ubiquitin-protein ligase that plays an integral role in the host innate immune response to viruses. It has been shown to inhibit the replication of a number of viruses, including HIV-1, hepatitis B, and influenza A. TRIM22 acts as a suppressor of basal HIV-1 long terminal repeat (LTR)-driven transcription by preventing transcription factor specificity protein 1 (Sp1) binding to the HIV-1 promoter. It also controls FoxO4 activity and cell survival by directing Toll-like receptor 3 (TLR3)-stimulated cells toward type I interferon (IFN) type I gene induction or apoptosis. Moreover, TRIM22 can activate the noncanonical nuclear factor-kappaB (NF-kappaB) pathway by activating I kappa B kinase alpha (IKKalpha). It also regulates nucleotide binding oligomerization domain containing 2 (NOD2)-dependent activation of interferon-beta signaling and nuclear factor-kappaB. TRIM34, also termed interferon-responsive finger protein 1, or RING finger protein 21 (RNF21), may function as an antiviral protein that contributes to the defense against retroviral infections. TRIM38, also known as RING finger protein 15 (RNF15) or zinc finger protein RoRet, is an E3 ubiquitin-protein ligase that promotes K63- and K48-linked ubiquitination of cellular proteins and also catalyzes self-ubiquitination. It negatively regulates tumor necrosis factor alpha (TNF-alpha) and interleukin-1beta-triggered nuclear factor-kappaB (NF-kappaB) activation by mediating lysosomal-dependent degradation of transforming growth factor beta (TGFbeta)-activated kinase 1 (TAK1)-binding protein (TAB)2/3, two critical components of the TAK1 kinase complex. It also inhibits TLR3/4-mediated activation of NF-kappaB and interferon regulatory factor 3 (IRF3) by mediating ubiquitin-proteasomal degradation of TNF receptor-associated factor 6 (Traf6) and NAK-associated protein 1 (Nap1), respectively. Moreover, TRIM38 negatively regulates TLR3-mediated interferon beta (IFN-beta) signaling by targeting ubiquitin-proteasomal degradation of TIR domain-containing adaptor inducing IFN-beta (TRIF). It functions as a valid target for autoantibodies in primary Sjogren's Syndrome.

Pssm-ID: 380819 [Multi-domain] Cd Length: 40 Bit Score: 49.42 E-value: 2.78e-08

                         10        20        30        40
                 ....*....|....*....|....*....|....*....|
gi 767912484  70 DLCQEHHEPLKLFCQKDQSPICVVCRESREHRLHRVLPAE 109
Cdd:cd19761    1 DHCEHHGEKLLLFCQEDGKVICWLCERSQEHRGHHTFLLE 40

Bbox2_TRIM20

cd19771

B-box-type 2 zinc finger found in tripartite motif-containing protein TRIM20 and similar ...

71-107

3.21e-08

B-box-type 2 zinc finger found in tripartite motif-containing protein TRIM20 and similar proteins; TRIM20, also termed Pyrin, or Marenostrin (MEFV), is involved in the regulation of innate immunity and the inflammatory response in response to IFNG/IFN-gamma. TRIM20 belongs to unclassified TRIM family of proteins that do not have RING fingers and thus lack the characteristic tripartite (RING (R), B-box, and coiled coil (CC)) RBCC motif. It contains a pyrin domain, a Bbox2 zinc finger, and a C-terminal SPRY/B30.2 domain. The type 2 B-box (Bbox2) zinc finger is characterized by a CHC3H2 zinc-binding consensus motif.

Pssm-ID: 380829 [Multi-domain] Cd Length: 39 Bit Score: 49.39 E-value: 3.21e-08

                         10        20        30
                 ....*....|....*....|....*....|....*..
gi 767912484  71 LCQEHHEPLKLFCQKDQSPICVVCRESREHRLHRVLP 107
Cdd:cd19771    2 QCKLHLEQLKLFCEDHREPICLICQLSQEHQGHRVRP 38

zf-B_box

pfam00643

B-box zinc finger;

67-107

6.51e-08

B-box zinc finger;

Pssm-ID: 459886 [Multi-domain] Cd Length: 42 Bit Score: 48.62 E-value: 6.51e-08

                          10        20        30        40
                  ....*....|....*....|....*....|....*....|..
gi 767912484   67 QKQDLCQEHH-EPLKLFCQKDQSPICVVCRESrEHRLHRVLP 107
Cdd:pfam00643   1 SKERLCPEHEeEPLTLYCNDCQELLCEECSVG-EHRGHTVVP 41

SPRY_PRY_TRIM1

cd13739

PRY/SPRY domain of tripartite motif-binding protein 1 (TRIM1) or MID2; This domain, consisting ...

305-431

7.89e-08

PRY/SPRY domain of tripartite motif-binding protein 1 (TRIM1) or MID2; This domain, consisting of the distinct N-terminal PRY subdomain followed by the SPRY subdomain, is found at the C-terminus of TRIM1 (also known as MID2 or midline 2). MID2 and its close homolog, TRIM18 (also known as MID1), both contain a B30.2-like domain at their C-terminus and a single fibronectin type III (FN3) motif between it and their N-terminal RBCC domain. MID2 and MID1 coiled-coil motifs mediate both homo- and heterodimerization, a prerequisite for association of the rapamycin-sensitive PP2A regulatory subunit Alpha 4 with microtubules. Mutations in MID1 have shown to cause Opitz syndrome, a disorder causing congenital anomalies such as cleft lip and palate as well as heart defects.

Pssm-ID: 293974 Cd Length: 170 Bit Score: 51.94 E-value: 7.89e-08

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484 305 DRFVAYPC--AVGQTAFSSGRHYWEVGMnitGDALW-ALGVCRDNVSRKDRVPKcpENGFWVvqLSKGTKYLSTFSALTP 381
Cdd:cd13739   35 ERFSGTGCygAAGNIFIDSGCHYWEVVV---GSSTWyAIGIAYKSAPKNEWIGK--NSSSWV--FSRCNNNFVVRHNNKE 107

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|..
gi 767912484 382 VMLMEPPS--HMGIFLDFEAGEVSFYSVSDGSHLHTYsQATFPGPLQPFFCL 431
Cdd:cd13739  108 MLVDVPPQlkRLGVLLDYDNNMLSFYDPANSLHLHTF-EVSFILPVCPTFTI 158

SPRY_PRY_TRIM16

cd12890

PRY/SPRY domain in tripartite motif-containing protein 16 (TRIM16); This domain, consisting of ...

266-440

8.47e-08

PRY/SPRY domain in tripartite motif-containing protein 16 (TRIM16); This domain, consisting of the distinct N-terminal PRY subdomain followed by the SPRY subdomain, is found at the C-terminus of TRIM16 and TRIM-like proteins. TRIM16 (also known as estrogen-responsive B box protein or EBBP) does not possess a RING domain like the other TRIM proteins, but contains two B-box domains and can heterodimerize with other TRIM proteins such as TRIM24, Promyelocytic leukemia (PML) protein and Midline-1 (MID1 or TRIM18). It is a regulator of keratinocyte differentiation and a tumor suppressor in retinoid-sensitive neuroblastoma. It has been shown that loss of TRIM16 expression plays an important role in the development of cutaneous squamous cell carcinoma (SCC) and is a determinant of retinoid sensitivity. TRIM16 also has E3 ubiquitin ligase activity.

Pssm-ID: 293948 Cd Length: 182 Bit Score: 52.08 E-value: 8.47e-08

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484 266 FLEDVVP---DATSAYPYLLLYESrQRRYLGSSP-EGSGFCSKDRFVAYPCAVGQTAFSSGRHYWEVgmNITGDALWaLG 341
Cdd:cd12890    4 FLKYAYPltfDPDTAHRYLRLTED-NRKVTNTTPwEHPYPDHPERFEHWRQVLSQQSLYLGRYYFEV--EISGEGTY-VG 79

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484 342 VCRDNVSRK--DRVPKCPENGF-WVVQLskGTKYLSTFSALTPVML-MEPPSHMGIFLDFEAGEVSFYSVSDGSH--LHT 415
Cdd:cd12890   80 LTYKSIDRKgsESNSCISGNNFsWCLQW--NGKEFSAWHSDVETPLkKGPFTRLGIYLDYPGGTLSFYGVEDDGMtlLHK 157

                        170       180
                 ....*....|....*....|....*
gi 767912484 416 YsQATFPGPLQPFFCLgaPKSGQMV 440
Cdd:cd12890  158 F-QCKFTEPLYPAFWL--SKKENAV 179

SPRY

cd11709

SPRY domain; SPRY domains, first identified in the SP1A kinase of Dictyostelium and rabbit ...

322-439

1.12e-07

SPRY domain; SPRY domains, first identified in the SP1A kinase of Dictyostelium and rabbit Ryanodine receptor (hence the name), are homologous to B30.2. SPRY domains have been identified in at least 11 protein families, covering a wide range of functions, including regulation of cytokine signaling (SOCS), RNA metabolism (DDX1 and hnRNP), immunity to retroviruses (TRIM5alpha), intracellular calcium release (ryanodine receptors or RyR) and regulatory and developmental processes (HERC1 and Ash2L). B30.2 also contains residues in the N-terminus that form a distinct PRY domain structure; i.e. B30.2 domain consists of PRY and SPRY subdomains. B30.2 domains comprise the C-terminus of three protein families: BTNs (receptor glycoproteins of immunoglobulin superfamily); several TRIM proteins (composed of RING/B-box/coiled-coil or RBCC core); Stonutoxin (secreted poisonous protein of the stonefish Synanceia horrida). TRIM/RBCC proteins are involved in a variety of processes, including apoptosis, cell cycle regulation, cell growth, senescence, viral response, meiosis, cell differentiation, and vesicular transport. Genes belonging to this family are implicated in several human diseases that vary from cancer to rare genetic syndromes. The PRY-SPRY domain in these TRIM families is suggested to serve as the target binding site. While SPRY domains are evolutionarily ancient, B30.2 domains are a more recent adaptation where the SPRY/PRY combination is a possible component of immune defense. Mutations found in the SPRY-containing proteins have shown to cause Mediterranean fever and Opitz syndrome.

Pssm-ID: 293931 Cd Length: 118 Bit Score: 50.12 E-value: 1.12e-07

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484 322 GRHYWEVGMNITGDALWALGVCRDNVSRKDRVPKCPENGFWVV---QLSKGTKYLSTFSALTPvmlmEPPSHMGIFLDFE 398
Cdd:cd11709    1 GKWYWEVRVDSGNGGLIQVGWATKSFSLDGEGGVGDDEESWGYdgsRLRKGHGGSSGPGGRPW----KSGDVVGCLLDLD 76

                         90       100       110       120
                 ....*....|....*....|....*....|....*....|...
gi 767912484 399 AGEVSFYsvSDGSHLHTYSQ--ATFPGPLQPFFCLGAPKSGQM 439
Cdd:cd11709   77 EGTLSFS--LNGKDLGVAFTnlFLKGGGLYPAVSLGSGQGVTI 117

Bbox2_TRIM21_C-IV

cd19772

B-box-type 2 zinc finger found in tripartite motif-containing protein 21 (TRIM21) and similar ...

72-109

1.31e-07

B-box-type 2 zinc finger found in tripartite motif-containing protein 21 (TRIM21) and similar proteins; TRIM21, also known as 52 kDa Ro protein, 52 kDa ribonucleoprotein autoantigen Ro/SS-A, Ro(SS-A), RING finger protein 81 (RNF81), or Sjoegren's syndrome type A antigen (SS-A), is a ubiquitously expressed E3 ubiquitin-protein ligase and a high affinity antibody receptor uniquely expressed in the cytosol of mammalian cells. As a cytosolic Fc receptor, TRIM21 binds the Fc of virus-associated antibodies and targets the complex in the cytosol for proteasomal degradation in a process known as antibody-dependent intracellular neutralization (ADIN), and provides an intracellular immune response to protect host defense against pathogen infection. It shows remarkably broad isotype specificity as it does not only bind IgG, but also IgM and IgA. Moreover, TRIM21 promotes the cytosolic DNA sensor cGAS and the cytosolic RNA sensor RIG-I sensing of viral genomes during infection by antibody-opsonized virus. It stimulates inflammatory signaling and activates innate transcription factors, such as nuclear factor-kappaB (NF-kappaB). TRIM21 also plays an essential role in p62-regulated redox homeostasis, suggesting a viable target for treating pathological conditions resulting from oxidative damage. Furthermore, TRIM21 may have implications for various autoimmune diseases associated uncontrolled antiviral signaling through the regulation of Nmi-IFI35 complex-mediated inhibition of innate antiviral response. TRIM21 belongs to the C-IV subclass of TRIM (tripartite motif) family of proteins that are defined by their N-terminal RBCC (RING, Bbox, and coiled coil) domains, including three consecutive zinc-binding domains, a RING finger, Bbox2, and a coiled coil region, as well as a B30.2/SPRY (SplA and ryanodine receptor) domain positioned C-terminal to the RBCC domain. The type 2 B-box (Bbox2) zinc finger is characterized by a CHC3H2 zinc-binding consensus motif.

Pssm-ID: 380830 [Multi-domain] Cd Length: 40 Bit Score: 47.49 E-value: 1.31e-07

                         10        20        30
                 ....*....|....*....|....*....|....*...
gi 767912484  72 CQEHHEPLKLFCQKDQSPICVVCRESREHRLHRVLPAE 109
Cdd:cd19772    3 CAVHGERLHLFCEEDQKALCLVCAQSQKHRDHAMVPIE 40

Bbox2_TRIM43-like

cd19783

B-box-type 2 zinc finger found in tripartite motif-containing proteins TRIM43, TRIM48, TRIM49, ...

71-118

2.67e-07

B-box-type 2 zinc finger found in tripartite motif-containing proteins TRIM43, TRIM48, TRIM49, TRIM51, TRIM64, TRIM77 and similar proteins; The family includes a group of closely related uncharacterized tripartite motif-containing proteins, TRIM43, TRIM43B, TRIM48/RNF101, TRIM49/RNF18, TRIM49B, TRIM49C/TRIM49L2, TRIM49D/TRIM49L, TRIM51/SPRYD5, TRIM64, TRIM64B, TRIM64C, and TRIM77, whose biological functions remain unclear. TRIM49, also known as testis-specific RING-finger protein, has moderate similarity with SS-A/Ro52 antigen, suggesting it may be one of target proteins of autoantibodies in the sera of patients with these autoimmune disorders. All family members (except for TRIM51) belong to the C-IV subclass of TRIM (tripartite motif) family of proteins that are defined by their N-terminal RBCC (RING, Bbox, and coiled coil) domains, including three consecutive zinc-binding domains, a RING finger, Bbox2, and a coiled coil region, as well as a SPRY/B30.2 domain positioned C-terminal to the RBCC domain. TRIM51 belongs to unclassified TRIM (tripartite motif) family of proteins that do not have RING fingers. The type 2 B-box (Bbox2) zinc finger is characterized by a CHC3H2 zinc-binding consensus motif.

Pssm-ID: 380841 [Multi-domain] Cd Length: 53 Bit Score: 47.16 E-value: 2.67e-07

                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....*...
gi 767912484  71 LCQEHHEPLKLFCQKDQSPICVVCRESREHRLHRVLPAEEAVQGYKLK 118
Cdd:cd19783    6 ICGTHRETKKLFCEADKSLLCLLCSSSQEHRAHRHYPIEWAAEEHREK 53

Bbox2_TRIM50-like

cd19787

B-box-type 2 zinc finger found in tripartite motif-containing protein TRIM50, TRIM73, TRIM74 ...

72-107

5.38e-07

B-box-type 2 zinc finger found in tripartite motif-containing protein TRIM50, TRIM73, TRIM74 and similar proteins; TRIM50 is a stomach-specific E3 ubiquitin-protein ligase, encoded by the Williams-Beuren syndrome (WBS) TRIM50 gene, which regulates vesicular trafficking for acid secretion in gastric parietal cells. It colocalizes, interacts with, and increases the level of p62/SQSTM1, a multifunctional adaptor protein implicated in various cellular processes including the autophagy clearance of polyubiquitinated protein aggregates. It also promotes the formation and clearance of aggresome-associated polyubiquitinated proteins through the interaction with the histone deacetylase 6 (HDAC6), a tubulin specific deacetylase that regulates microtubule-dependent aggresome formation. TRIM50 can be acetylated by PCAF and p300. TRIM50 belongs to the C-IV subclass of TRIM (tripartite motif) family of proteins that are defined by their N-terminal RBCC (RING, Bbox, and coiled coil) domains, including three consecutive zinc-binding domains, a RING finger, Bbox2, and a coiled coil region, as well as a B30.2/SPRY (SplA and ryanodine receptor) domain positioned C-terminal to the RBCC domain. The type 2 B-box (Bbox2) zinc finger is characterized by a CHC3H2 zinc-binding consensus motif. The family also includes two paralogs of TRIM50, tripartite motif-containing protein 73 (TRIM73), also known as tripartite motif-containing protein 50B (TRIM50B), and tripartite motif-containing protein 74 (TRIM74), also known as tripartite motif-containing protein 50C (TRIM50C), both of which are WBS-related genes encoding proteins and may also act as E3 ligases. In contrast with TRIM50, TRIM73 and TRIM74 belong to the C-V subclass of TRIM family of proteins that are defined by the N-terminal RBCC domains only.

Pssm-ID: 380845 [Multi-domain] Cd Length: 39 Bit Score: 45.94 E-value: 5.38e-07

                         10        20        30
                 ....*....|....*....|....*....|....*.
gi 767912484  72 CQEHHEPLKLFCQKDQSPICVVCRESREHRLHRVLP 107
Cdd:cd19787    3 CPHHHNPLSLFCEKDQEVICGLCGLIGSHRQHKITP 38

RING-HC_TRIM25_C-IV

cd16597

RING finger, HC subclass, found in tripartite motif-containing protein TRIM25 and similar ...

1-58

5.57e-07

RING finger, HC subclass, found in tripartite motif-containing protein TRIM25 and similar proteins; TRIM25, also known as estrogen-responsive finger protein (EFP), RING finger protein 147 (RNF147), or RING-type E3 ubiquitin transferase, is an E3 ubiquitin/ISG15 ligase that is induced by estrogen and is therefore particularly abundant in placenta and uterus. TRIM25 regulates various cellular processes through E3 ubiquitin ligase activity, transferring ubiquitin and ISG15 to target proteins. It mediates K63-linked polyubiquitination of retinoic acid inducible gene I (RIG-I) that is crucial for downstream antiviral interferon signaling. It is also required for melanoma differentiation-associated gene 5 (MDA5) and mitochondrial antiviral signaling (MAVS, also known as IPS-1, VISA, Cardiff) mediated activation of nuclear factor-kappaB (NF-kappaB) and interferon production. Upon UV irradiation, TRIM25 interacts with mono-ubiquitinated PCNA and promotes its ISG15 modification (ISGylation), suggesting a crucial role in termination of error-prone translesion DNA synthesis. TRIM25 also functions as a novel regulator of p53 and Mdm2. It enhances p53 and Mdm2 abundance by inhibiting their ubiquitination and degradation in 26S proteasomes. Meanwhile, it inhibits p53's transcriptional activity and dampens the response to DNA damage, and is essential for medaka development and this dependence is rescued by silencing of p53. Moreover, TRIM25 is involved in the host cellular innate immune response against retroviral infection. It interferes with the late stage of feline leukemia virus (FeLV) replication. Furthermore, TRIM25 acts as an oncogene in gastric cancer. Its blockade by RNA interference inhibits migration and invasion of gastric cancer cells through transforming growth factor-beta (TGF-beta) signaling, suggesting it presents a novel target for the detection and treatment of gastric cancer. In addition, TRIM25 acts as an RNA-specific activator for Lin28a/TuT4-mediated uridylation. TRIM25 belongs to the C-IV subclass of TRIM (tripartite motif) family of proteins that are defined by their N-terminal RBCC (RING, Bbox, and coiled coil) domains, including three consecutive zinc-binding domains, a C3HC4-type RING-HC finger, Bbox1 and Bbox2, and a coiled coil region, as well as a B30.2/SPRY (SplA and ryanodine receptor) domain positioned C-terminal to the RBCC domain.

Pssm-ID: 438259 [Multi-domain] Cd Length: 71 Bit Score: 46.92 E-value: 5.57e-07

                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*....
gi 767912484   1 MTTCGHNFCRACIQLSWEkargkkgrRKRKGSFPCPECREMSPQR-NLLPNRLLTKVAE 58
Cdd:cd16597   20 TLPCGHNFCGVCIEKTWD--------SQHGSEYSCPQCRATFPRRpELHKNTVLRNIVE 70

RING-HC_TRIM35_C-IV

cd16599

RING finger, HC subclass, found in tripartite motif-containing protein 35 (TRIM35) and similar ...

4-58

5.76e-07

RING finger, HC subclass, found in tripartite motif-containing protein 35 (TRIM35) and similar proteins; TRIM35, also known as hemopoietic lineage switch protein 5 (HLS5), is a putative hepatocellular carcinoma (HCC) suppressor that inhibits phosphorylation of pyruvate kinase isoform M2 (PKM2), which is involved in aerobic glycolysis of cancer cells and further suppresses the Warburg effect and tumorigenicity in HCC. It also negatively regulates Toll-like receptor 7 (TLR7)- and TLR9-mediated type I interferon production by suppressing the stability of interferon regulatory factor 7 (IRF7). Moreover, TRIM35 regulates erythroid differentiation by modulating globin transcription factor 1 (GATA-1) activity. TRIM35 belongs to the C-IV subclass of the TRIM (tripartite motif) family of proteins that are defined by their N-terminal RBCC (RING, Bbox, and coiled coil) domains, including three consecutive zinc-binding domains, a C3HC4-type RING-HC finger, Bbox1 and Bbox2, and a coiled coil region, as well as a B30.2/SPRY (SplA and ryanodine receptor) domain positioned C-terminal to the RBCC domain.

Pssm-ID: 438261 [Multi-domain] Cd Length: 66 Bit Score: 46.69 E-value: 5.76e-07

                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*
gi 767912484   4 CGHNFCRACIQLSWEKargkkgrrkrKGSFPCPECREMSPQRNLLPNRLLTKVAE 58
Cdd:cd16599   22 CGHNFCKGCVSRSWER----------QPRAPCPVCKEASSSDDLRTNHTLNNLVE 66

RING-HC_TRIM41-like_C-IV

cd16602

RING finger, HC subclass, found in tripartite motif-containing proteins TRIM41, TRIM52 and ...

4-50

6.06e-07

RING finger, HC subclass, found in tripartite motif-containing proteins TRIM41, TRIM52 and similar proteins; TRIM41 and TRIM52, two closely related tripartite motif-containing proteins, have dramatically expanded RING domains compared with the rest of the TRIM family proteins. TRIM41 belongs to the C-IV subclass of the TRIM (tripartite motif) family of proteins that are defined by their N-terminal RBCC (RING, Bbox, and coiled coil) domains, including three consecutive zinc-binding domains, a C3HC4-type RING-HC finger, Bbox1 and Bbox2, and a coiled coil region, as well as a B30.2/SPRY (SplA and ryanodine receptor) domain positioned C-terminal to the RBCC domain. In contrast, TRIM52 lacks the putative viral recognition SPRY/B30.2 domain, and thus has been classified to the C-V subclass of the TRIM family that contains only RBCC domains. TRIM41, also known as RING finger-interacting protein with C kinase (RINCK), is an E3 ubiquitin-protein ligase that promotes the ubiquitination of protein kinase C (PKC) isozymes in cells. It specifically recognizes the C1 domain of PKC isozymes. It controls the amplitude of PKC signaling by controlling the amount of PKC in the cell. TRIM52, also known as RING finger protein 102 (RNF102), is encoded by a novel, noncanonical antiviral TRIM52 gene in primate genomes with unique specificity determined by the rapidly evolving RING domain.

Pssm-ID: 438264 [Multi-domain] Cd Length: 53 Bit Score: 46.07 E-value: 6.06e-07

                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....*..
gi 767912484   4 CGHNFCRACIQLSWekargkkgrrkrkgSFPCPECREMSPQRNLLPN 50
Cdd:cd16602   21 CGHNFCRVCVTQLW--------------GFTCPQCRKSFPRRSFRPN 53

RING-HC_TRIM43-like_C-IV

cd16603

RING finger, HC subclass, found in tripartite motif-containing proteins TRIM43, TRIM48, TRIM49, ...

4-50

6.92e-07

RING finger, HC subclass, found in tripartite motif-containing proteins TRIM43, TRIM48, TRIM49, TRIM51, TRIM64 and similar proteins; The family includes a group of closely related uncharacterized tripartite motif-containing proteins, TRIM43, TRIM43B, TRIM48/RNF101, TRIM49/RNF18, TRIM49B, TRIM49C/TRIM49L2, TRIM49D/TRIM49L, TRIM51/SPRYD5, TRIM64, TRIM64B, and TRIM64C, whose biological function remain unclear. TRIM49, also known as testis-specific RING-finger protein, has moderate similarity with SS-A/Ro52 antigen, suggesting it may be one of the target proteins of autoantibodies in the sera of patients with these autoimmune disorders. All family members belong to the C-IV subclass of the TRIM (tripartite motif) family of proteins that are defined by their N-terminal RBCC (RING, Bbox, and coiled coil) domains as well as a SPRY/B30.2 domain positioned C-terminal to the RBCC domain. In RBCC region, they all have a C3HC4-type RING-HC finger.

Pssm-ID: 438265 [Multi-domain] Cd Length: 59 Bit Score: 46.32 E-value: 6.92e-07

                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....*..
gi 767912484   4 CGHNFCRACIQLSWEKARGKKGrrkrkgsfpCPECREMSPQRNLLPN 50
Cdd:cd16603   22 CGHSFCRPCLYLNWQDIPFLAQ---------CPECRKTTEQRNLKTN 59

SPRY_PRY_TRIM76

cd12898

PRY/SPRY domain in tripartite motif-containing protein 76 (TRIM76), also called ...

276-436

2.36e-06

PRY/SPRY domain in tripartite motif-containing protein 76 (TRIM76), also called cardiomyopathy-associated protein 5; This domain, consisting of the distinct N-terminal PRY subdomain followed by the SPRY subdomain, is found at the C-terminus of TRIM76, a Class I TRIM protein. TRIM76 (also known as cardiomyopathy-associated protein 5 or CMYA5 or myospryn or SPRYD2) is a muscle-specific member of the TRIM superfamily, but lacks the RING domain. It has been suggested that TRIM76 is involved in two distinct processes, protein kinase A signaling and vesicular trafficking. It has also been implicated in Duchenne muscular dystrophy and cardiac disease; gene polymorphism of TRIM76 is associated with left ventricular wall thickness in patients with hypertension while its interactions with M-band titin and calpain 3 link it to tibial and limb-girdle muscular dystrophies.

Pssm-ID: 293955 Cd Length: 171 Bit Score: 47.62 E-value: 2.36e-06

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484 276 SAYPYLLLYESRQR-RYLGSSPEGSgfcskDRFVAYPCAVGQTAFSSGRHYWEVgmNITGDALWALGVCRDNVSRkdRVP 354
Cdd:cd12898   10 TAHPALHISSDRGTvIYFHERRRKM-----SSLTECPSVLGEELPSCGQYYWET--TVTRCPAYRLGICSSSASQ--AGA 80

                         90       100       110       120       130       140       150       160
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484 355 KCPENGFWVVQ---LSKGTKYLSTFSAL-TPVMLMEPPSHMGIFLDFEAGEVSFYSVSDGSHLHTYSQaTFPGPLQPFFC 430
Cdd:cd12898   81 LGEGSTSWCLHcvpTSEPCRYTLLHSGIvSDVFVTERPARVGTLLDYNNGRLIFINAESGQLLGIFRH-RFAQPCHPAFA 159


                 ....*.
gi 767912484 431 LGAPKS 436
Cdd:cd12898  160 LEKPGK 165

RING-HC_TRIM65_C-IV

cd16609

RING finger, HC subclass, found in tripartite motif-containing protein TRIM65 and similar ...

4-45

3.18e-06

RING finger, HC subclass, found in tripartite motif-containing protein TRIM65 and similar proteins; TRIM65 is an E3 ubiquitin-protein ligase that interacts with the innate immune receptor MDA5, enhancing its ability to stimulate interferon-beta signaling. It functions as a potential oncogenic protein that negatively regulates p53 through ubiquitination, providing insight into the development of novel approaches targeting TRIM65 for non-small cell lung carcinoma (NSCLC) treatment, and also overcoming chemotherapy resistance. Moreover, TRIM65 negatively regulates microRNA-driven suppression of mRNA translation by targeting TNRC6 proteins for ubiquitination and degradation. TRIM65 belongs to the C-IV subclass of the TRIM (tripartite motif) family of proteins that are defined by their N-terminal RBCC (RING, Bbox, and coiled coil) domains, including three consecutive zinc-binding domains, a C3HC4-type RING-HC finger, Bbox1 and Bbox2, and a coiled coil region, as well as a B30.2/SPRY (SplA and ryanodine receptor) domain positioned C-terminal to the RBCC domain.

Pssm-ID: 438271 [Multi-domain] Cd Length: 58 Bit Score: 44.28 E-value: 3.18e-06

                         10        20        30        40
                 ....*....|....*....|....*....|....*....|..
gi 767912484   4 CGHNFCRACIQLSWEKArgkkgrrkRKGSFPCPECREMSPQR 45
Cdd:cd16609   21 CQHSFCRACIEDHWRQK--------DEGSFSCPECRAPFPEG 54

RING-HC_TRIM39_C-IV

cd16601

RING finger, HC subclass, found in tripartite motif-containing protein 39 (TRIM39) and similar ...

3-38

5.51e-06

RING finger, HC subclass, found in tripartite motif-containing protein 39 (TRIM39) and similar proteins; TRIM39, also known as RING finger protein 23 (RNF23) or testis-abundant finger protein, is an E3 ubiquitin-protein ligase that plays a role in controlling DNA damage-induced apoptosis through inhibition of the anaphase promoting complex (APC/C), a multiprotein ubiquitin ligase that controls multiple cell cycle regulators, including cyclins, geminin, and others. TRIM39 also functions as a regulator of several key processes in the proliferative cycle. It directly regulates p53 stability. It modulates cell cycle progression and DNA damage responses via stabilizing p21. Moreover, TRIM39 negatively regulates the nuclear factor kappaB (NFkappaB)-mediated signaling pathway through stabilization of Cactin, an inhibitor of NFkappaB- and Toll-like receptor (TLR)-mediated transcription, which is induced by inflammatory stimulants such as tumor necrosis factor alpha. Furthermore, TRIM39 is a MOAP-1-binding protein that can promote apoptosis signaling through stabilization of MOAP-1 via the inhibition of its poly-ubiquitination process. TRIM39 belongs to the C-IV subclass of the TRIM (tripartite motif) family of proteins that are defined by their N-terminal RBCC (RING, Bbox, and coiled coil) domains, including three consecutive zinc-binding domains, a C3HC4-type RING-HC finger, Bbox1 and Bbox2, and a coiled coil region, as well as a B30.2/SPRY (SplA and ryanodine receptor) domain positioned C-terminal to the RBCC domain.

Pssm-ID: 438263 [Multi-domain] Cd Length: 44 Bit Score: 43.24 E-value: 5.51e-06

                         10        20        30
                 ....*....|....*....|....*....|....*.
gi 767912484   3 TCGHNFCRACIQLSWEKArgkkgrrkrKGSFPCPEC 38
Cdd:cd16601   18 ECGHNFCRACITRFWEEL---------DGDFPCPQC 44

RING-HC_TRIM4_C-IV

cd16590

RING finger, HC subclass, found in tripartite motif-containing protein TRIM4 and similar ...

4-50

5.54e-06

RING finger, HC subclass, found in tripartite motif-containing protein TRIM4 and similar proteins; TRIM4 belongs to the C-IV subclass of the TRIM (tripartite motif) family of proteins that are defined by their N-terminal RBCC (RING, Bbox, and coiled coil) domains, including three consecutive zinc-binding domains, a C3HC4-type RING-HC finger, Bbox2, and a coiled coil region, as well as a SPRY/B30.2 domain positioned C-terminal to the RBCC domain. TRIM4, also known as RING finger protein 87 (RNF87), is a cytoplasmic E3 ubiquitin-protein ligase that has recently evolved and is present only in higher mammals. It transiently interacts with mitochondria, induces mitochondrial aggregation and sensitizes the cells to hydrogen peroxide (H2O2) induced death. Its interaction with peroxiredoxin 1 (PRX1) is critical for the regulation of H2O2 induced cell death. Moreover, TRIM4 functions as a positive regulator of RIG-I-mediated type I interferon induction. It regulates the K63-linked ubiquitination of RIG-1 and assembly of antiviral signaling complex at the mitochondria.

Pssm-ID: 438252 [Multi-domain] Cd Length: 61 Bit Score: 43.87 E-value: 5.54e-06

                         10        20        30        40
                 ....*....|....*....|....*....|....*....|....*..
gi 767912484   4 CGHNFCRACIQLSWEKArgkkgrrkrKGSFPCPECREMSPQRNLLPN 50
Cdd:cd16590   24 CGHNFCRGCLHRNWAPG---------GGPFPCPECRHPSAPAALRPN 61

RING-HC_TRIM60-like_C-IV

cd16607

RING finger, HC subclass, found in tripartite motif-containing proteins TRIM60, TRIM61, TRIM75 ...

2-39

7.83e-06

RING finger, HC subclass, found in tripartite motif-containing proteins TRIM60, TRIM61, TRIM75 and similar proteins; TRIM60, also known as RING finger protein 129 (RNF129) or RING finger protein 33 (RNF33), is a cytoplasmic protein expressed in the testis. It may play an important role in the spermatogenesis process, the development of the preimplantation embryo, and in testicular functions. RNF33 interacts with the cytoplasmic kinesin motor proteins KIF3A and KIF3B suggesting possible contribution to cargo movement along the microtubule in the expressed sites. It is also involved in spermatogenesis in Sertoli cells under the regulation of nuclear factor-kappaB (NF-kappaB). TRIM75 mainly localizes within spindles, suggesting it may function in spindle organization and thereby affect meiosis. Both TRIM60 and TRIM75 belong the C-IV subclass of the TRIM (tripartite motif) family of proteins that are defined by their N-terminal RBCC (RING, Bbox, and coiled coil) domains, including three consecutive zinc-binding domains, a C3HC4-type RING-HC finger, a B2-box, and two coiled coil domains, as well as a PRY domain and a B30.2/SPRY (SplA and ryanodine receptor) domain positioned C-terminal to the RBCC domain. In contrast, TRIM61 belongs to the C-V subclass of the TRIM family that contains RBCC domains only. Its biological function remains unclear.

Pssm-ID: 438269 [Multi-domain] Cd Length: 48 Bit Score: 42.79 E-value: 7.83e-06

                         10        20        30
                 ....*....|....*....|....*....|....*...
gi 767912484   2 TTCGHNFCRACIQLSWEkargkkgrrKRKGSFPCPECR 39
Cdd:cd16607   17 INCGHNFCRSCISMSWK---------DLQDTFPCPVCR 45

SPRY_PRY_TRIM25

cd13736

PRY/SPRY domain in tripartite motif-containing domain 25 (TRIM25); This domain, consisting of ...

306-429

1.03e-05

PRY/SPRY domain in tripartite motif-containing domain 25 (TRIM25); This domain, consisting of the distinct N-terminal PRY subdomain followed by the SPRY subdomain, is found at the C-terminus of TRIM25 proteins (composed of RING/B-box/coiled-coil core and also known as RBCC proteins). TRIM25 (also called Efp) ubiquitinates the N terminus of the viral RNA receptor retinoic acid-inducible gene-I (RIG-I) in response to viral infection, leading to activation of the RIG-I signaling pathway, thus resulting in type I interferon production to limit viral replication. It has been shown that the influenza A virus targets TRIM25 and disables its antiviral function.

Pssm-ID: 293971 [Multi-domain] Cd Length: 169 Bit Score: 45.64 E-value: 1.03e-05

                         10        20        30        40        50        60        70        80
                 ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 767912484 306 RFVAYPCAVGQTAFSSGRHYWEVGM---NITGdalwaLGVCRDNVSRK---DRVPKCPENgfWVVQLSKgTKYLSTFSAL 379
Cdd:cd13736   36 RFTYCSQVLGLHCFKQGIHYWEVELqknNFCG-----VGICYGSMDRQgpeSRLGRNSES--WCVEWFN-VKISAWHNNV 107

                         90       100       110       120       130
                 ....*....|....*....|....*....|....*....|....*....|
gi 767912484 380 TPVMLMEPPSHMGIFLDFEAGEVSFYSVSDGSHLHTYSQATFPGPLQPFF 429
Cdd:cd13736  108 EKTLPSTKATRVGVLLNCDHGFVIFFAVQDKVHLMYKFKVDFTEALYPAF 157

Bbox_SF

cd00021

B-box-type zinc finger superfamily; The B-box-type zinc finger is a short zinc binding domain ...

71-107

1.45e-05

B-box-type zinc finger superfamily; The B-box-type zinc finger is a short zinc binding domain of around 40 amino acid residues in length. It has been found in transcription factors, ribonucleoproteins and proto-oncoproteins, such as in TRIM (tripartite motif) proteins that consist of an N-terminal RING finger (originally called an A-box), followed by 1-2 B-box domains and a coiled-coil domain (also called RBCC for Ring, B-box, Coiled-Coil). The B-box-type zinc finger often presents in combination with other motifs, like RING zinc finger, NHL motif, coiled-coil or RFP domain in functionally unrelated proteins, most likely mediating protein-protein interactions. Based on different consensus sequences and the spacing of the 7-8 zinc-binding residues, B-box-type zinc fingers can be divided into two groups, type 1 (Bbox1: C6H2) and type 2 (Bbox2: CHC3H2).

Pssm-ID: 380813 [Multi-domain] Cd Length: 39 Bit Score: 41.82 E-value: 1.45e-05

                         10        20        30
                 ....*....|....*....|....*....|....*...
gi 767912484  71 LCQEHHE-PLKLFCQKDQSPICVVCRESREHRLHRVLP 107
Cdd:cd00021    1 MCQEHDEeKANKYCVTCEVLYCALCKKSGAHPDHEVAP 38

Bbox2_TRIM65-like

cd19793

B-box-type 2 zinc finger found in tripartite motif-containing protein 65 (TRIM65), B box and ...

70-110

7.68e-05

B-box-type 2 zinc finger found in tripartite motif-containing protein 65 (TRIM65), B box and SPRY domain-containing protein (BSPRY) and similar proteins; The family includes TRIM65 and BSPRY. TRIM65 is an E3 ubiquitin-protein ligase that interacts with the innate immune receptor MDA5 enhancing its ability to stimulate interferon-beta signaling. It functions as a potential oncogenic protein that negatively regulates p53 through ubiquitination, providing insight into development of novel approaches targeting TRIM65 for non-small cell lung carcinoma (NSCLC) treatment, and also overcoming chemotherapy resistance. Moreover, TRIM65 negatively regulates microRNA-driven suppression of mRNA translation by targeting TNRC6 proteins for ubiquitination and degradation. BSPRY is a regulatory protein for maintaining calcium homeostasis. It may regulate epithelial calcium transport by inhibiting TRPV5 activity. The type 2 B-box (Bbox2) zinc finger is characterized by a CHC3H2 zinc-binding consensus motif.

Pssm-ID: 380851 Cd Length: 43 Bit Score: 39.98 E-value: 7.68e-05

                         10        20        30        40
                 ....*....|....*....|....*....|....*....|.
gi 767912484  70 DLCQEHHEPLKLFCQKDQSPICVVCRESREHRLHRVLPAEE 110
Cdd:cd19793    1 ELCPEHGRELELYCRTEKRCVCAQCASKGECRGHRVTLLEE 41

RING-HC_TRIM77_C-IV

cd16543

RING finger, HC subclass, found in tripartite motif-containing protein 77 (TRIM77) and similar ...

4-45

1.10e-04

RING finger, HC subclass, found in tripartite motif-containing protein 77 (TRIM77) and similar proteins; TRIM77 belongs to the C-IV subclass of the TRIM (tripartite motif) family of proteins that are defined by their N-terminal RBCC (RING, Bbox, and coiled coil) domains, including two consecutive zinc-binding domains, a C3HC4-type RING-HC finger and Bbox2, as well as a SPRY/B30.2 domain positioned C-terminal to the RBCC domain.

Pssm-ID: 438205 [Multi-domain] Cd Length: 54 Bit Score: 39.68 E-value: 1.10e-04

                         10        20        30        40
                 ....*....|....*....|....*....|....*....|..
gi 767912484   4 CGHNFCRACIQLSWEKARGKKGrrkrkgsfpCPECREMSPQR 45
Cdd:cd16543   21 CGHSFCMNCITLLWDRKQGVPS---------CPQCRESFPPR 53

RING-HC_TRIM5-like_C-IV

cd16591

RING finger, HC subclass, found in tripartite motif-containing proteins TRIM5, TRIM6, TRIM22, ...

4-58

2.39e-04

RING finger, HC subclass, found in tripartite motif-containing proteins TRIM5, TRIM6, TRIM22, TRIM34 and similar proteins; TRIM5, TRIM6, TRIM22, and TRIM34, four closely related tripartite motif-containing proteins, belong to the C-IV subclass of the TRIM (tripartite motif) family of proteins that are defined by their N-terminal RBCC (RING, Bbox, and coiled coil) domains, including three consecutive zinc-binding domains, a C3HC4-type RING-HC finger, Bbox1 and Bbox2, and a coiled coil region, as well as a B30.2/SPRY (SplA and ryanodine receptor) domain positioned C-terminal to the RBCC domain. TRIM5, also known as RING finger protein 88 (RNF88), is a capsid-specific restriction factor that prevents infection from non-host-adapted retroviruses in a species-specific manner by binding to and destabilizing the retroviral capsid lattice before reverse transcription is completed. Its retroviral restriction activity correlates with the ability to activate TAK1-dependent innate immune signaling. TRIM5 also acts as a pattern recognition receptor that activates innate immune signaling in response to the retroviral capsid lattice. Moreover, TRIM5 plays a role in regulating autophagy through activation of autophagy regulator BECN1 by causing its dissociation from its inhibitors BCL2 and TAB2. It also plays a role in autophagy by acting as a selective autophagy receptor which recognizes and targets HIV-1 capsid protein p24 for autophagic destruction. TRIM6, also known as RING finger protein 89 (RNF89), is an E3-ubiquitin ligase that cooperates with the E2-ubiquitin conjugase UbE2K to catalyze the synthesis of unanchored K48-linked polyubiquitin chains, and further stimulates the interferon-I kappa B kinase epsilon (IKKepsilon) kinase-mediated antiviral response. It also regulates the transcriptional activity of Myc during the maintenance of embryonic stem (ES) cell pluripotency, and may act as a novel regulator for Myc-mediated transcription in ES cells. TRIM22, also known as 50 kDa-stimulated trans-acting factor (Staf-50) or RING finger protein 94 (RNF94), is an E3 ubiquitin-protein ligase that plays an integral role in the host innate immune response to viruses. It has been shown to inhibit the replication of a number of viruses, including HIV-1, hepatitis B, and influenza A. TRIM22 acts as a suppressor of basal HIV-1 long terminal repeat (LTR)-driven transcription by preventing the transcription factor specificity protein 1 (Sp1) binding to the HIV-1 promoter. It also controls FoxO4 activity and cell survival by directing Toll-like receptor 3 (TLR3)-stimulated cells toward type I interferon (IFN) type I gene induction or apoptosis. Moreover, TRIM22 can activate the noncanonical nuclear factor-kappaB (NF-kappaB) pathway by activating I kappa B kinase alpha (IKKalpha). It also regulates nucleotide binding oligomerization domain containing 2 (NOD2)-dependent activation of interferon-beta signaling and nuclear factor-kappaB. TRIM34, also known as interferon-responsive finger protein 1 or RING finger protein 21 (RNF21), may function as antiviral protein that contribute to the defense against retroviral infections.

Pssm-ID: 438253 [Multi-domain] Cd Length: 72 Bit Score: 39.35 E-value: 2.39e-04

                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|....*
gi 767912484   4 CGHNFCRACIQLSWEKARGKKGRRKrkgsfpCPECREMSPQRNLLPNRLLTKVAE 58
Cdd:cd16591   24 CGHSFCQACITANHKESVNQEGESS------CPVCRTSYQPENLRPNRHLANIVE 72

RING-HC_RNF39

cd16592

RING finger, HC subclass, found in RING finger protein 39 (RNF39) and similar proteins; RNF39, ...

1-43

3.27e-04

RING finger, HC subclass, found in RING finger protein 39 (RNF39) and similar proteins; RNF39, also called protein HZFw, may play a role in prolonged long term-potentiation (LTP) maintenance. It is involved in the etiology of Behcet's disease (BD). It may also be involved in HIV-1 replication. RNF39 acts as an E3 ubiquitin ligase that inhibits retinoic acid-inducible gene-I (RIG-I)-like receptor (RLR) pathways by mediating K48-linked ubiquitination and proteasomal degradation of DDX3X (DEAD-box RNA helicase 3, X-linked). RNF39 contains a typical C3HC4-type RING-HC finger.

Pssm-ID: 438254 [Multi-domain] Cd Length: 58 Bit Score: 38.59 E-value: 3.27e-04

                         10        20        30        40
                 ....*....|....*....|....*....|....*....|...
gi 767912484   1 MTTCGHNFCRACIQLSWEkarGKKGRRKRKGSFPCPECREMSP 43
Cdd:cd16592   19 ILDCEHSFCRACIARHWG---QEAMEGNGAEGVFCPQCGEPCP 58

Bbox2_TRIM46_C-I

cd19786

B-box-type 2 zinc finger found in tripartite motif-containing protein 46 (TRIM46) and similar ...

71-113

5.87e-04

B-box-type 2 zinc finger found in tripartite motif-containing protein 46 (TRIM46) and similar proteins; TRIM46, also known as gene Y protein (GeneY) or tripartite, fibronectin type-III and C-terminal SPRY motif protein (TRIFIC), is a microtubule-associated protein that specifically localizes to the proximal axon, partly overlaps with the axon initial segment (AIS) at later stages, and organizes uniform microtubule orientation in axons. It controls neuronal polarity and axon specification by driving the formation of parallel microtubule arrays. TRIM46 belongs to the C-I subclass of TRIM (tripartite motif) family of proteins, which are defined by their N-terminal RBCC (RING, Bbox, and coiled coil) domains, including three consecutive zinc-binding domains, a RING finger, Bbox1 and Bbox2, and a coiled coil region, as well as a COS (carboxyl-terminal subgroup one signature) box, a fibronectin type III (FN3) domain, and a B30.2/SPRY (SplA and ryanodine receptor) domain positioned C-terminal to the RBCC domain. The type 2 B-box (Bbox2) zinc finger is characterized by a CHC3H2 zinc-binding consensus motif.

Pssm-ID: 380844 [Multi-domain] Cd Length: 46 Bit Score: 37.59 E-value: 5.87e-04

                         10        20        30        40
                 ....*....|....*....|....*....|....*....|...
gi 767912484  71 LCQEHHEPLKLFCQKDQSPICVVCRESREHRLHRVLPAEEAVQ 113
Cdd:cd19786    4 MCPEHKEEVTHYCKTCQRLVCQLCRVRRTHAGHKITPVLSAYQ 46

Bbox2_TRIM44

cd19784

B-box-type 2 zinc finger found in tripartite motif-containing protein 44 (TRIM44) and similar ...

72-106

1.03e-03

B-box-type 2 zinc finger found in tripartite motif-containing protein 44 (TRIM44) and similar proteins; TRIM44, also termed protein DIPB, functions as a critical regulator in tumor metastasis and progression. TRIM44 belongs to an unclassified TRIM (tripartite motif) family of proteins that do not have RING fingers and thus lack the characteristic tripartite (RING (R), B-box, and coiled coil (CC)) RBCC motif. It contains a Bbox2 domain. The type 2 B-box (Bbox2) zinc finger is characterized by a CHC3H2 zinc-binding consensus motif.

Pssm-ID: 380842 [Multi-domain] Cd Length: 39 Bit Score: 36.67 E-value: 1.03e-03

                         10        20        30
                 ....*....|....*....|....*....|....*
gi 767912484  72 CQEHHEPLKLFCQKDQSPICVVCRESREHRLHRVL 106
Cdd:cd19784    3 CPEHGQELSLYCKEDEKIICVLCAVIGAHRQHQLI 37

zf-C3HC4

pfam00097

Zinc finger, C3HC4 type (RING finger); The C3HC4 type zinc-finger (RING finger) is a ...

1-38

2.63e-03

Pssm-ID: 395049 [Multi-domain] Cd Length: 40 Bit Score: 35.41 E-value: 2.63e-03

                          10        20        30
                  ....*....|....*....|....*....|....*...
gi 767912484    1 MTTCGHNFCRACIQLSWEKargkkgrrkrkGSFPCPEC 38
Cdd:pfam00097  14 LLPCGHLFCSKCIRSWLES-----------GNVTCPLC 40

RING-HC_TRIM26_C-IV

cd16598

RING finger, HC subclass, found in tripartite motif-containing protein 26 (TRIM26) and similar ...

4-53

2.83e-03

RING finger, HC subclass, found in tripartite motif-containing protein 26 (TRIM26) and similar proteins; TRIM26, also known as acid finger protein (AFP), RING finger protein 95 (RNF95), or zinc finger protein 173 (ZNF173), is an E3 ubiquitin-protein ligase that negatively regulates interferon-beta production and antiviral response through polyubiquitination and degradation of nuclear transcription factor IRF3. It functions as an important regulator for RNA virus-triggered innate immune response by bridging TBK1 to NEMO (NF-kappaB essential modulator, also known as IKKgamma) and mediating TBK1 activation. It also acts as a novel tumor suppressor of hepatocellular carcinoma by regulating cancer cell proliferation, colony forming ability, migration, and invasion. TRIM26 belongs the C-IV subclass of the TRIM (tripartite motif) family of proteins that are defined by their N-terminal RBCC (RING, Bbox, and coiled coil) domains, including three consecutive zinc-binding domains, a C3HC4-type RING-HC finger, a B-box, and two coiled coil domains, as well as a B30.2/SPRY (SplA and ryanodine receptor) domain positioned C-terminal to the RBCC domain.

Pssm-ID: 438260 [Multi-domain] Cd Length: 64 Bit Score: 36.30 E-value: 2.83e-03

                         10        20        30        40        50
                 ....*....|....*....|....*....|....*....|....*....|
gi 767912484   4 CGHNFCRACIQLSWEKArgkkgrrKRKGSFPCPECREMSPQRNLLPNRLL 53
Cdd:cd16598   22 CGHNFCRSCITDYCPIS-------GGHERPVCPLCRKPFKKENIRPNWQL 64

RING-HC_TRIM8_C-V

cd16580

RING finger, HC subclass, found in tripartite motif-containing protein 8 (TRIM8) and similar ...

4-45

3.29e-03

RING finger, HC subclass, found in tripartite motif-containing protein 8 (TRIM8) and similar proteins; TRIM8, also known as glioblastoma-expressed RING finger protein (GERP) or RING finger protein 27 (RNF27), is a probable E3 ubiquitin-protein ligase that may promote proteasomal degradation of suppressor of cytokine signaling 1 (SOCS1) and further regulate interferon-gamma signaling. It functions as a new p53 modulator that stabilizes p53 impairing its association with MDM2 and inducing the reduction of cell proliferation. TRIM8 deficit dramatically impairs p53 stabilization and activation in response to chemotherapeutic drugs. TRIM8 also modulates tumor necrosis factor-alpha (TNFalpha) and interleukin-1beta (IL-1beta)-triggered nuclear factor-kappaB (NF- kappa B) activation by targeting transforming growth factor beta (TGFbeta) activated kinase 1 (TAK1) for K63-linked polyubiquitination. Moreover, TRIM8 modulates translocation of phosphorylated STAT3 into the nucleus through interaction with Hsp90beta and consequently regulates transcription of Nanog in embryonic stem cells. It also interacts with protein inhibitor of activated STAT3 (PIAS3), which inhibits IL-6-dependent activation of STAT3. TRIM8 belongs to the C-V subclass of the TRIM (tripartite motif) family of proteins that are defined by their N-terminal RBCC (RING, Bbox, and coiled coil) domains, including three consecutive zinc-binding domains, a C3HC4-type RING-HC finger, Bbox1 and Bbox2, and a coiled coil domain, as well as an uncharacterized region positioned C-terminal to the RBCC domain. The coiled coil domain is required for homodimerization and the region immediately C-terminal to the RING motif is sufficient to mediate the interaction with SOCS1.

Pssm-ID: 438242 [Multi-domain] Cd Length: 67 Bit Score: 36.02 E-value: 3.29e-03

                         10        20        30        40
                 ....*....|....*....|....*....|....*....|..
gi 767912484   4 CGHNFCRACIQLSWEKARGKKGrrkrkgsfpCPECREMSPQR 45
Cdd:cd16580   29 CKHNFCRGCIGEAWAKDAGLVR---------CPECNQAYNQK 61

RING-HC

cd16449

HC subclass of RING (RING-HC) finger and its variants; The RING finger is a specialized type ...

1-38

5.04e-03

HC subclass of RING (RING-HC) finger and its variants; The RING finger is a specialized type of Zn-finger of 40 to 60 residues that binds two atoms of zinc. It is defined by the "cross-brace" motif that chelates zinc atoms by eight amino acid residues, typically Cys or His, arranged in a characteristic spacing. Canonical RING motifs have been categorized into two major subclasses, RING-HC (C3HC4-type) and RING-H2 (C3H2C3-type), according to their Cys/His content. There are also many variants of RING fingers. Some have a different Cys/His pattern. Some lack a single Cys or His residue at typical Zn ligand positions, especially, the fourth or eighth zinc ligand is prevalently exchanged for an Asp, which can chelate Zn in a RING finger as well. This family corresponds to the HC subclass of RING (RING-HC) fingers that are characterized by containing C3HC4-type canonical RING-HC fingers or noncanonical RING-HC finger variants, including C4C4-, C3HC3D-, C2H2C4-, and C3HC5-type modified RING-HC fingers. The canonical RING-HC finger has been defined as C-X2-C-X(9-39)-C-X(1-3)-H-X(2-3)-C-X2-C-X(4-48)-C-X2-C. It binds two Zn ions in a unique "cross-brace" arrangement, which distinguishes it from tandem zinc fingers and other similar motifs. RING-HC fingers can be found in a group of diverse proteins with a variety of cellular functions, including oncogenesis, development, viral replication, signal transduction, the cell cycle, and apoptosis. Many of them are ubiquitin-protein ligases (E3s) that serve as scaffolds for binding to ubiquitin-conjugating enzymes (E2s, also referred to as ubiquitin carrier proteins or UBCs) in close proximity to substrate proteins, which enables efficient transfer of ubiquitin from E2 to the substrates.

Pssm-ID: 438113 [Multi-domain] Cd Length: 41 Bit Score: 34.77 E-value: 5.04e-03

                         10        20        30
                 ....*....|....*....|....*....|....*...
gi 767912484   1 MTTCGHNFCRACIQLSWEKargkkgrrkrkGSFPCPEC 38
Cdd:cd16449   15 LLPCGHVFCRECIRRLLES-----------GSIKCPIC 41

Bbox2_BSPRY

cd19834

B-box-type 2 zinc finger found in B box and SPRY domain-containing protein (BSPRY) and ...

70-110

5.75e-03

B-box-type 2 zinc finger found in B box and SPRY domain-containing protein (BSPRY) and similar proteins; BSPRY is a regulatory protein for maintaining calcium homeostasis. It may regulate epithelial calcium transport by inhibiting TRPV5 activity. BSPRY is composed of a B-box, an alpha-helical coiled coil and a SPRY domain. The B-box motif shows high sequence similarity with B-Box-type zinc finger 2 found in tripartite motif-containing proteins (TRIMs). The type 2 B-box (Bbox2) zinc finger is characterized by a CHC3H2 zinc-binding consensus motif.

Pssm-ID: 380892 Cd Length: 43 Bit Score: 34.67 E-value: 5.75e-03

                         10        20        30        40
                 ....*....|....*....|....*....|....*....|.
gi 767912484  70 DLCQEHHEPLKLFCQKDQSPICVVCRESREHRLHRVLPAEE 110
Cdd:cd19834    1 DLCPDHELELDWFCSTERRLVCAQCASLGTCRGHRVTPLEE 41

Bbox2_TRIM25_C-IV

cd19776

B-box-type 2 zinc finger found in tripartite motif-containing protein 25 (TRIM25) and similar ...

72-94

6.72e-03

B-box-type 2 zinc finger found in tripartite motif-containing protein 25 (TRIM25) and similar proteins; TRIM25, also termed estrogen-responsive finger protein (EFP), or ubiquitin/ISG15-conjugating enzyme TRIM25, or zinc finger protein 147 (ZNF147), or E3 ubiquitin/ISG15 ligase TRIM25, is induced by estrogen and particularly abundant in placenta and uterus. It has been implicated in cell proliferation, protein modification, and the retinoic acid inducible gene I (RIG-I)-mediated antiviral signaling pathway. It functions as an E3-ubiquitin ligase able to transfer ubiquitin and ISG15 to target proteins. It binds to mono-ubiquitinated PCNA and promotes the ISG15 modification (ISGylation) of PCNA, suggesting a crucial role in termination of error-prone translesion DNA synthesis. TRIM25 also enhances p53 and Mdm2 abundance by inhibiting their ubiquitination and degradation in 26S proteasomes. It suppresses p53's transcriptional activity and dampens the response to DNA damage. Upon deubiquitylation by ubiquitin-specific peptidase 15 (USP15), it mediates K63-linked polyubiquitination of RIG-I that is crucial for downstream antiviral interferon signaling. TRIM25 is required for melanoma differentiation-associated gene 5 (MDA5) and mitochondrial antiviral signaling (MAVS, also known as IPS-1, VISA, Cardiff) mediated activation of nuclear factor-kappaB (NF- kappa B) and interferon production. It is an RNA binding protein acting as RNA-specific activator for Lin28a/TuT4-mediated uridylation. TRIM25 belongs to the C-IV subclass of TRIM (tripartite motif) family of proteins that are defined by their N-terminal RBCC (RING, Bbox, and coiled coil) domains, including three consecutive zinc-binding domains, a RING finger, Bbox1 and Bbox2, and a coiled coil region, as well as a SPRY/B30.2 domain positioned C-terminal to the RBCC domain. The type 2 B-box (Bbox2) zinc finger is characterized by a CHC3H2 zinc-binding consensus motif.

Pssm-ID: 380834 Cd Length: 38 Bit Score: 34.29 E-value: 6.72e-03

                         10        20
                 ....*....|....*....|...
gi 767912484  72 CQEHHEPLKLFCQKDQSPICVVC 94
Cdd:cd19776    3 CTQHGKLLEFYCKSHSLCICSTC 25

zf-RING_UBOX

pfam13445

RING-type zinc-finger; This zinc-finger is a typical RING-type of plant ubiquitin ligases.

1-36

6.86e-03

RING-type zinc-finger; This zinc-finger is a typical RING-type of plant ubiquitin ligases.

Pssm-ID: 463881 [Multi-domain] Cd Length: 38 Bit Score: 34.30 E-value: 6.86e-03

                          10        20        30
                  ....*....|....*....|....*....|....*.
gi 767912484    1 MTTCGHNFCRACIQLSWEKargkkgrrkRKGSFPCP 36
Cdd:pfam13445  12 VLPCGHTFCRECLEEMSQK---------KGGKFKCP 38

Bbox2_TRIM65_C-IV

cd19835

B-box-type 2 zinc finger found in tripartite motif-containing protein 65 (TRIM65) and similar ...

71-110

7.08e-03

B-box-type 2 zinc finger found in tripartite motif-containing protein 65 (TRIM65) and similar proteins; TRIM65 is an E3 ubiquitin-protein ligase that interacts with the innate immune receptor MDA5 enhancing its ability to stimulate interferon-beta signaling. It functions as a potential oncogenic protein that negatively regulates p53 through ubiquitination, providing insight into development of novel approaches targeting TRIM65 for non-small cell lung carcinoma (NSCLC) treatment, and also overcoming chemotherapy resistance. Moreover, TRIM65 negatively regulates microRNA-driven suppression of mRNA translation by targeting TNRC6 proteins for ubiquitination and degradation. TRIM65 belongs to the C-IV subclass of TRIM (tripartite motif) family of proteins that are defined by their N-terminal RBCC (RING, Bbox, and coiled coil) domains, including three consecutive zinc-binding domains, a RING finger, Bbox2, and a coiled coil region, as well as a B30.2/SPRY (SplA and ryanodine receptor) domain positioned C-terminal to the RBCC domain. The type 2 B-box (Bbox2) zinc finger is characterized by a CHC3H2 zinc-binding consensus motif.

Pssm-ID: 380893 [Multi-domain] Cd Length: 42 Bit Score: 34.32 E-value: 7.08e-03

                         10        20        30        40
                 ....*....|....*....|....*....|....*....|..
gi 767912484  71 LCQEHHEPLKLFCQKDQSPICVVC--RESREHrlHRVLPAEE 110
Cdd:cd19835    2 LCQRHGRPLELYCRTEKRCVCCKCtvKECRNH--NRVLLEEE 41

zf-C3HC4_4

pfam15227

zinc finger of C3HC4-type, RING; This is a family of primate-specific Ret finger protein-like ...

4-38

8.40e-03

zinc finger of C3HC4-type, RING; This is a family of primate-specific Ret finger protein-like (RFPL) zinc-fingers of the C3HC4 type. Ret finger protein-like proteins are primate-specific target genes of Pax6, a key transcription factor for pancreas, eye and neocortex development. This domain is likely to be DNA-binding. This zinc-finger domain together with the RDM domain, pfam11002, forms a large zinc-finger structure of the RING/U-Box superfamily. RING-containing proteins are known to exert an E3 ubiquitin protein ligase activity with the zinc-finger structure being mandatory for binding to the E2 ubiquitin-conjugating enzyme.

Pssm-ID: 464570 [Multi-domain] Cd Length: 42 Bit Score: 34.33 E-value: 8.40e-03

                          10        20        30
                  ....*....|....*....|....*....|....*
gi 767912484    4 CGHNFCRACIQLSWEKArgkkgrrkRKGSFPCPEC 38
Cdd:pfam15227  16 CGHSFCLSCINSLQKEP--------DGESLLCPQC 42

Bbox2_MID

cd19758

B-box-type 2 zinc finger found in midline (MID) family; The MID family includes MID1 and MID2. ...

71-105

8.64e-03

B-box-type 2 zinc finger found in midline (MID) family; The MID family includes MID1 and MID2. MID1, also known as midin, midline 1 RING finger protein, putative transcription factor XPRF, RING finger protein 59 (RNF59), or tripartite motif-containing protein 18 (TRIM18), is a microtubule-associated E3 ubiquitin-protein ligase implicated in epithelial-mesenchymal differentiation, cell migration and adhesion, and programmed cell death along specific regions of the ventral midline during embryogenesis. MID2, also known as midin-2, midline defect 2, RING finger protein 60 (RNF60), or tripartite motif-containing protein 1 (TRIM1), is highly related to MID1. It associates with the microtubule network and may at least partially compensate for the loss of MID1. Both MID1 and MID2 interacts with Alpha 4, which is a regulatory subunit of PP2-type phosphatases, such as PP2A, and an integral component of the rapamycin-sensitive signaling pathway. They also play a central role in the regulation of granule exocytosis, and functional redundancy exists between MID1 and MID2 in cytotoxic lymphocytes (CTL). Both MID1 and MID2 belong to the C-I subclass of TRIM (tripartite motif) family of proteins that are defined by their N-terminal RBCC (RING, Bbox, and coiled coil) domains, including three consecutive zinc-binding domains, a RING finger, Bbox1 and Bbox2, and a coiled coil region, as well as a COS (carboxyl-terminal subgroup one signature) box, a fibronectin type III (FN3) domain, and a B30.2/SPRY (SplA and ryanodine receptor) domain positioned C-terminal to the RBCC domain. The type 2 B-box (Bbox2) zinc finger is characterized by a CHC3H2 zinc-binding consensus motif.

Pssm-ID: 380816 Cd Length: 40 Bit Score: 33.98 E-value: 8.64e-03

                         10        20        30
                 ....*....|....*....|....*....|....*.
gi 767912484  71 LCQEH-HEPLKLFCQKDQSPICVVCRESREHRLHRV 105
Cdd:cd19758    2 MCSEHeEEKVNMYCLTDDQLICSLCKLVGKHKDHEV 37

Bbox2_TRIM59_C-XI

cd19790

B-box-type 2 zinc finger found in tripartite motif-containing protein 59 (TRIM59) and similar ...

72-103

8.90e-03

B-box-type 2 zinc finger found in tripartite motif-containing protein 59 (TRIM59) and similar proteins; TRIM59, also known as TRIM57, or RING finger protein 104 (RNF104) or tumor suppressor TSBF-1, is a putative E3 ubiquitin-protein ligase that functions as a novel multiple cancer biomarker for immunohistochemical detection of early tumorigenesis. It is upregulated in gastric cancer and promotes gastric carcinogenesis by interacting with and targeting the P53 tumor suppressor for its ubiquitination and degradation. It also acts as a novel accessory molecule involved in cytotoxicity of BCG-activated macrophages (BAM). Moreover, TRIM59 may serve as a multifunctional regulator for innate immune signaling pathways. It interacts with ECSIT and negatively regulates nuclear factor-kappaB (NF- kappa B) and interferon regulatory factor (IRF)-3/7-mediated signal pathways. TRIM59 belongs to the C-XI subclass of TRIM (tripartite motif) family of proteins that are defined by an N-terminal RBCC (RING, Bbox, and coiled coil) domains, including three consecutive zinc-binding domains, a RING finger, Bbox2, and a coiled coil region. In addition, TRIM59 contains a C-terminal transmembrane domain.

Pssm-ID: 380848 [Multi-domain] Cd Length: 40 Bit Score: 33.97 E-value: 8.90e-03

                         10        20        30
                 ....*....|....*....|....*....|...
gi 767912484  72 CQEHH-EPLKLFCQKDQSPICVVCRESREHRLH 103
Cdd:cd19790    3 CPEHYrQPLNLFCLLDRKLICGQCLTVGQHQGH 35

SPRY_PRY_TRIM67_9

cd12889

PRY/SPRY domain in tripartite motif-containing proteins, TRIM9 and TRIM67; This domain, ...

310-349

9.33e-03

PRY/SPRY domain in tripartite motif-containing proteins, TRIM9 and TRIM67; This domain, consisting of the distinct N-terminal PRY subdomain followed by the SPRY subdomain, is found at the C-terminus of TRIM9 proteins. TRIM9 protein is expressed mainly in the cerebral cortex, and functions as an E3 ubiquitin ligase. It has been shown that TRIM9 is localized to the neurons in the normal human brain and its immunoreactivity in affected brain areas in Parkinson's disease and dementia with Lewy bodies is severely decreased, possibly playing an important role in the regulation of neuronal function and participating in pathological process of Lewy body disease through its ligase. TRIM67 negatively regulates Ras activity via degradation of 80K-H, leading to neural differentiation, including neuritogenesis.

Pssm-ID: 293947 Cd Length: 172 Bit Score: 36.84 E-value: 9.33e-03

                         10        20        30        40
                 ....*....|....*....|....*....|....*....|.
gi 767912484 310 YPCAVGQTAFSSGRHYWEVGMN-ITGDALWALGVCRDNVSR 349
Cdd:cd12889   37 DRVVLGSVGFSRGVHYWEVTIDrYDGHPDPAFGVARIDVNK 77

Blast search parameters

Data Source:	Precalculated data, version = cdd.v.3.21
Preset Options:	Database: CDSEARCH/cdd Low complexity filter: no Composition Based Adjustment: yes E-value threshold: 0.01