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Conserved domains on  [gi|1039790976|ref|XP_017168502|]
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calcium uptake protein 3, mitochondrial isoform X2 [Mus musculus]

Protein Classification

calcium uptake protein 3( domain architecture ID 11610845)

mitochondrial calcium uptake protein 3 (MICU3) may play a role in mitochondrial calcium uptake

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
EFh_MICU3 cd16175
EF-hand, calcium binding motif, found in calcium uptake protein 3, mitochondrial (MICU3) and ...
 60-327 3.53e-54

EF-hand, calcium binding motif, found in calcium uptake protein 3, mitochondrial (MICU3) and similar proteins; MICU3, also termed EF-hand domain-containing family member A2 (EFHA2), is a paralog of MICU1 and notably found in the central nervous system (CNS) and skeletal muscle. At present, the precise molecular function of MICU3 remains unclear. It likely has a role in mitochondrial calcium handling. MICU3 contains an N-terminal mitochondrial targeting sequence (MTS) as well as two evolutionarily conserved canonical Ca2+-binding EF-hands separated by a long stretch of residues predicted to form alpha-helices.


:

Pssm-ID: 320083 [Multi-domain]  Cd Length: 128  Bit Score: 174.24  E-value: 3.53e-54
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039790976  60 GFRIAFNMFDTDGNEMVDKKEFLVLQEIFRkknekretkgdeekramlrlqlygyhsptnsvlktdagelvsrsywdtlr 139
Cdd:cd16175     1 GFRIAFNMFDTDGNEMVDKKEFLVLQEIFR-------------------------------------------------- 30

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039790976 140 rstsqalfsdlaeradditslvadtTLLVHFFGKKGKAELNFEDFYRFMDNLQTEVleieflsysngmntiseedfahil 219
Cdd:cd16175    31 -------------------------TLLVHFFGKKGKAELNFEDFYRFMDNLQTEV------------------------ 61

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039790976 220 lrytnventsvflenvrysiseekgitfdefrsffqflnnlEDFAIALNMYNFASRSIGQDEFKRAVYVATGLKLSPHLV 299
Cdd:cd16175    62 -----------------------------------------EDFTIAMRMYTFADRSISQDEFARAVKVCTGLKLSPHLV 100

                         250       260
                  ....*....|....*....|....*...
gi 1039790976 300 NTVFKIFDVDKDDQLSYKEFIGIMKDRL 327
Cdd:cd16175   101 NTVFKIFDVDGDGQLSYKEFIGIMKDRL 128
 
Name Accession Description Interval E-value
EFh_MICU3 cd16175
EF-hand, calcium binding motif, found in calcium uptake protein 3, mitochondrial (MICU3) and ...
 60-327 3.53e-54

EF-hand, calcium binding motif, found in calcium uptake protein 3, mitochondrial (MICU3) and similar proteins; MICU3, also termed EF-hand domain-containing family member A2 (EFHA2), is a paralog of MICU1 and notably found in the central nervous system (CNS) and skeletal muscle. At present, the precise molecular function of MICU3 remains unclear. It likely has a role in mitochondrial calcium handling. MICU3 contains an N-terminal mitochondrial targeting sequence (MTS) as well as two evolutionarily conserved canonical Ca2+-binding EF-hands separated by a long stretch of residues predicted to form alpha-helices.


Pssm-ID: 320083 [Multi-domain]  Cd Length: 128  Bit Score: 174.24  E-value: 3.53e-54
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039790976  60 GFRIAFNMFDTDGNEMVDKKEFLVLQEIFRkknekretkgdeekramlrlqlygyhsptnsvlktdagelvsrsywdtlr 139
Cdd:cd16175     1 GFRIAFNMFDTDGNEMVDKKEFLVLQEIFR-------------------------------------------------- 30

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039790976 140 rstsqalfsdlaeradditslvadtTLLVHFFGKKGKAELNFEDFYRFMDNLQTEVleieflsysngmntiseedfahil 219
Cdd:cd16175    31 -------------------------TLLVHFFGKKGKAELNFEDFYRFMDNLQTEV------------------------ 61

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039790976 220 lrytnventsvflenvrysiseekgitfdefrsffqflnnlEDFAIALNMYNFASRSIGQDEFKRAVYVATGLKLSPHLV 299
Cdd:cd16175    62 -----------------------------------------EDFTIAMRMYTFADRSISQDEFARAVKVCTGLKLSPHLV 100

                         250       260
                  ....*....|....*....|....*...
gi 1039790976 300 NTVFKIFDVDKDDQLSYKEFIGIMKDRL 327
Cdd:cd16175   101 NTVFKIFDVDGDGQLSYKEFIGIMKDRL 128
EF-hand_7 pfam13499
EF-hand domain pair;
274-324 7.81e-05

EF-hand domain pair;


Pssm-ID: 463900 [Multi-domain]  Cd Length: 67  Bit Score: 40.31  E-value: 7.81e-05
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|..
gi 1039790976 274 SRSIGQDEFKRAVY-VATGLKLSPHLVNTVFKIFDVDKDDQLSYKEFIGIMK 324
Cdd:pfam13499  16 DGYLDVEELKKLLRkLEEGEPLSDEEVEELFKEFDLDKDGRISFEEFLELYS 67
FRQ1 COG5126
Ca2+-binding protein, EF-hand superfamily [Signal transduction mechanisms];
245-325 2.48e-04

Ca2+-binding protein, EF-hand superfamily [Signal transduction mechanisms];


Pssm-ID: 444056 [Multi-domain]  Cd Length: 137  Bit Score: 40.55  E-value: 2.48e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039790976 245 ITFDEFRSFFQFLNNLEDFAIALNMYNFA----SRSIGQDEFKRAVyvaTGLKLSPHLVNTVFKIFDVDKDDQLSYKEFI 320
Cdd:COG5126    50 ISREEFVAGMESLFEATVEPFARAAFDLLdtdgDGKISADEFRRLL---TALGVSEEEADELFARLDTDGDGKISFEEFV 126


                  ....*
gi 1039790976 321 GIMKD 325
Cdd:COG5126   127 AAVRD 131
 
Name Accession Description Interval E-value
EFh_MICU3 cd16175
EF-hand, calcium binding motif, found in calcium uptake protein 3, mitochondrial (MICU3) and ...
 60-327 3.53e-54

EF-hand, calcium binding motif, found in calcium uptake protein 3, mitochondrial (MICU3) and similar proteins; MICU3, also termed EF-hand domain-containing family member A2 (EFHA2), is a paralog of MICU1 and notably found in the central nervous system (CNS) and skeletal muscle. At present, the precise molecular function of MICU3 remains unclear. It likely has a role in mitochondrial calcium handling. MICU3 contains an N-terminal mitochondrial targeting sequence (MTS) as well as two evolutionarily conserved canonical Ca2+-binding EF-hands separated by a long stretch of residues predicted to form alpha-helices.


Pssm-ID: 320083 [Multi-domain]  Cd Length: 128  Bit Score: 174.24  E-value: 3.53e-54
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039790976  60 GFRIAFNMFDTDGNEMVDKKEFLVLQEIFRkknekretkgdeekramlrlqlygyhsptnsvlktdagelvsrsywdtlr 139
Cdd:cd16175     1 GFRIAFNMFDTDGNEMVDKKEFLVLQEIFR-------------------------------------------------- 30

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039790976 140 rstsqalfsdlaeradditslvadtTLLVHFFGKKGKAELNFEDFYRFMDNLQTEVleieflsysngmntiseedfahil 219
Cdd:cd16175    31 -------------------------TLLVHFFGKKGKAELNFEDFYRFMDNLQTEV------------------------ 61

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039790976 220 lrytnventsvflenvrysiseekgitfdefrsffqflnnlEDFAIALNMYNFASRSIGQDEFKRAVYVATGLKLSPHLV 299
Cdd:cd16175    62 -----------------------------------------EDFTIAMRMYTFADRSISQDEFARAVKVCTGLKLSPHLV 100

                         250       260
                  ....*....|....*....|....*...
gi 1039790976 300 NTVFKIFDVDKDDQLSYKEFIGIMKDRL 327
Cdd:cd16175   101 NTVFKIFDVDGDGQLSYKEFIGIMKDRL 128
EFh_MICU cd15900
EF-hand, calcium binding motif, found in mitochondrial calcium uptake proteins MICU1, MICU2, ...
 60-327 3.44e-50

EF-hand, calcium binding motif, found in mitochondrial calcium uptake proteins MICU1, MICU2, MICU3, and similar proteins; This family includes mitochondrial calcium uptake protein MICU1 and its two additional paralogs, MICU2 and MICU3. MICU1 localizes to the inner mitochondrial membrane (IMM). It functions as a gatekeeper of the mitochondrial calcium uniporter (MCU) and regulates MCU-mediated mitochondrial Ca2+ uptake, which is essential for maintaining mitochondrial homoeostasis. MICU1 and MICU2 are physically associated within the uniporter complex and are co-expressed across all tissues. They may play non-redundant roles in the regulation of the mitochondrial calcium uniporter. At present, the precise molecular function of MICU2 and MICU3 remain unclear. MICU2 may play possible roles in Ca2+ sensing and regulation of MCU, calcium buffering with a secondary impact on transport or assembly and stabilization of MCU. MICU3 likely has a role in mitochondrial calcium handling. All members in this family contains an N-terminal mitochondrial targeting sequence (MTS) as well as two evolutionarily conserved canonical Ca2+-binding EF-hands separated by a long stretch of residues predicted to form alpha-helices.


Pssm-ID: 320080 [Multi-domain]  Cd Length: 152  Bit Score: 164.71  E-value: 3.44e-50
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039790976  60 GFRIAFNMFDTDGNEMVDKKEFLVLQEIFRKKNEKRETKGDEEKRAMLRLQLygyhsptnsvlktdagelvsrsywdtlr 139
Cdd:cd15900     1 HFEIAFKMFDLDGDGELDKEEFNKVQSIIRSQTSVGQRHRDHTNGESTKLGM---------------------------- 52

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039790976 140 rstsqalfsdlaeradditslvaDTTLLVHFFGKKGKAELNFEDFYRFMDNLQTEVleieflsysngmntiseedfahil 219
Cdd:cd15900    53 -----------------------NSTLARYFFGKDGKQKLSIEKFLEFQENLQEEI------------------------ 85

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039790976 220 lrytnventsvflenvrysiseekgitfdefrsffqflnnlEDFAIALNMYNFASRSIGQDEFKRAVYVATGLKLSPHLV 299
Cdd:cd15900    86 -----------------------------------------DDVDTALTFYHLAGASIDRKTFKRAAKVVAGVELSDHVV 124

                         250       260
                  ....*....|....*....|....*...
gi 1039790976 300 NTVFKIFDVDKDDQLSYKEFIGIMKDRL 327
Cdd:cd15900   125 DVVFTIFDEDGDGILSHKEFISVMKDRL 152
EFh_MICU2 cd16174
EF-hand, calcium binding motif, found in calcium uptake protein 2, mitochondrial (MICU2) and ...
 60-327 2.05e-37

EF-hand, calcium binding motif, found in calcium uptake protein 2, mitochondrial (MICU2) and similar proteins; MICU2, also termed EF-hand domain-containing family member A1 (EFHA1), is a mitochondrial-localized paralog of MICU1. MICU2 and its paralog, MICU1, are physically associated within the mitochondrial calcium uniporter (MCU) complex and are co-expressed across all tissues. They may operate together with MCU to regulate the channel. At present, the precise molecular function of MICU2 remains unclear. It may play possible roles in Ca2+ sensing and regulation of MCU, calcium buffering with a secondary impact on transport or assembly and stabilization of MCU. MICU2 contains an N-terminal mitochondrial targeting sequence (MTS) as well as two evolutionarily conserved canonical Ca2+-binding EF-hands separated by a long stretch of residues predicted to form alpha-helices.


Pssm-ID: 320082 [Multi-domain]  Cd Length: 154  Bit Score: 131.92  E-value: 2.05e-37
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039790976  60 GFRIAFNMFDTDGNEMVDKKEFLVLQEIFRKKNEkretkgdeekramlrlqlygyhsptnsvLKTDAGELVSRsywdtlr 139
Cdd:cd16174     1 GFHIAFKMLDTDGNEQVEKREFFKLQKIIGKKDD----------------------------LMTQGGTETYQ------- 45

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039790976 140 rstsqalfsDLAERADDItslvaDTTLLVHFFGKKGKAELNFEDFYRFMDNLQTEVleieflsysngmntiseedfahil 219
Cdd:cd16174    46 ---------EASDNSDEV-----NTTLQVHFFGKDGNEKLQYKEFCRFMENLQTEV------------------------ 87

                         170       180       190       200       210       220       230       240
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039790976 220 lrytnventsvflenvrysiseekgitfdefrsffqflnnlEDFAIALNMYNFASRSIGQDEFKRAVYVATGLKLSPHLV 299
Cdd:cd16174    88 -----------------------------------------EDFAIAMKMFSEANRPIKLAEFKRAVKVATGQELSDNVL 126

                         250       260
                  ....*....|....*....|....*...
gi 1039790976 300 NTVFKIFDVDKDDQLSYKEFIGIMKDRL 327
Cdd:cd16174   127 DTVFKIFDLDGDDCLSHGEFLGVLKNRV 154
EFh_MICU1 cd16173
EF-hand, calcium binding motif, found in calcium uptake protein 1, mitochondrial (MICU1) and ...
 246-327 3.44e-07

EF-hand, calcium binding motif, found in calcium uptake protein 1, mitochondrial (MICU1) and similar proteins; MICU1, also termed atopy-related autoantigen CALC (ara CALC), or calcium-binding atopy-related autoantigen 1 (CBARA1), or Hom s 4, or EFHA3, localizes to the inner mitochondrial membrane (IMM). It functions as a gatekeeper of the mitochondrial calcium uniporter (MCU) and regulates MCU-mediated mitochondrial Ca2+ uptake, which is essential for maintaining mitochondrial homoeostasis. MICU1 and its paralog, MICU2, are physically associated within the uniporter complex and are co-expressed across all tissues. They may operate together with MCU to regulate the channel. The mutations in MICU1 are associated with neuromuscular abnormalities in children. MICU1 contains an N-terminal mitochondrial targeting sequence (MTS) as well as two evolutionarily conserved canonical Ca2+-binding EF-hands separated by a long stretch of residues predicted to form alpha-helices.


Pssm-ID: 320081 [Multi-domain]  Cd Length: 153  Bit Score: 49.25  E-value: 3.44e-07
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039790976 246 TFDEFRSFFQflNNLEDFAIALNMYNFASRSIGQDEFKRAVYVATGLKLSPHLVNTVFKIFDVDKDDQLSYKEFIGIMKD 325
Cdd:cd16173    74 NFLEFQRKLQ--HDVNDVDTALSFYHMAGASLDKVTMQQVARTVAKVELSDHVCDVVFALFDCDGNGELSNKEFVAIMKQ 151


                  ..
gi 1039790976 326 RL 327
Cdd:cd16173   152 RL 153
EF-hand_7 pfam13499
EF-hand domain pair;
274-324 7.81e-05

EF-hand domain pair;


Pssm-ID: 463900 [Multi-domain]  Cd Length: 67  Bit Score: 40.31  E-value: 7.81e-05
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|..
gi 1039790976 274 SRSIGQDEFKRAVY-VATGLKLSPHLVNTVFKIFDVDKDDQLSYKEFIGIMK 324
Cdd:pfam13499  16 DGYLDVEELKKLLRkLEEGEPLSDEEVEELFKEFDLDKDGRISFEEFLELYS 67
EF-hand_8 pfam13833
EF-hand domain pair;
274-326 2.17e-04

EF-hand domain pair;


Pssm-ID: 404678 [Multi-domain]  Cd Length: 54  Bit Score: 38.84  E-value: 2.17e-04
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|...
gi 1039790976 274 SRSIGQDEFKRAVYVATGLKLSPHLVNTVFKIFDVDKDDQLSYKEFIGIMKDR 326
Cdd:pfam13833   2 KGVITREELKRALALLGLKDLSEDEVDILFREFDTDGDGYISFDEFCVLLERR 54
FRQ1 COG5126
Ca2+-binding protein, EF-hand superfamily [Signal transduction mechanisms];
245-325 2.48e-04

Ca2+-binding protein, EF-hand superfamily [Signal transduction mechanisms];


Pssm-ID: 444056 [Multi-domain]  Cd Length: 137  Bit Score: 40.55  E-value: 2.48e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039790976 245 ITFDEFRSFFQFLNNLEDFAIALNMYNFA----SRSIGQDEFKRAVyvaTGLKLSPHLVNTVFKIFDVDKDDQLSYKEFI 320
Cdd:COG5126    50 ISREEFVAGMESLFEATVEPFARAAFDLLdtdgDGKISADEFRRLL---TALGVSEEEADELFARLDTDGDGKISFEEFV 126


                  ....*
gi 1039790976 321 GIMKD 325
Cdd:COG5126   127 AAVRD 131
EFh_PEF_ALG-2_like cd16185
EF-hand, calcium binding motif, found in homologs of mammalian apoptosis-linked gene 2 protein ...
 245-322 4.29e-04

EF-hand, calcium binding motif, found in homologs of mammalian apoptosis-linked gene 2 protein (ALG-2); The family includes some homologs of mammalian apoptosis-linked gene 2 protein (ALG-2) mainly found in lower eukaryotes, such as a parasitic protist Leishmarua major and a cellular slime mold Dictyostelium discoideum. These homologs contains five EF-hand motifs. Due to the presence of unfavorable residues at the Ca2+-coordinating positions, their non-canonical EF4 and EF5 hands may not bind Ca2+. Two Dictyostelium PEF proteins are the prototypes of this family. They may bind to cytoskeletal proteins and/or signal-transducing proteins localized to detergent-resistant membranes named lipid rafts, and occur as monomers or weak homo- or heterodimers like ALG-2. They can serve as a mediator for Ca2+ signaling-related Dictyostehum programmed cell death (PCD).


Pssm-ID: 320060 [Multi-domain]  Cd Length: 163  Bit Score: 40.28  E-value: 4.29e-04
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1039790976 245 ITFDEFRSFFQFLNNLEDfaiALNMYNfASRS--IGQDEFKRAVyVATGLKLSPHLVNTVFKIFDVDKDDQLSYKEFIGI 322
Cdd:cd16185    53 IDFEEFAALHQFLSNMQN---GFEQRD-TSRSgrLDANEVHEAL-AASGFQLDPPAFQALFRKFDPDRGGSLGFDDYIEL 127
EFh cd00051
EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal ...
 274-324 6.58e-04

EF-hand, calcium binding motif; A diverse superfamily of calcium sensors and calcium signal modulators; most examples in this alignment model have 2 active canonical EF hands. Ca2+ binding induces a conformational change in the EF-hand motif, leading to the activation or inactivation of target proteins. EF-hands tend to occur in pairs or higher copy numbers.


Pssm-ID: 238008 [Multi-domain]  Cd Length: 63  Bit Score: 37.53  E-value: 6.58e-04
                          10        20        30        40        50
                  ....*....|....*....|....*....|....*....|....*....|.
gi 1039790976 274 SRSIGQDEFKRAVyVATGLKLSPHLVNTVFKIFDVDKDDQLSYKEFIGIMK 324
Cdd:cd00051    14 DGTISADELKAAL-KSLGEGLSEEEIDEMIREVDKDGDGKIDFEEFLELMA 63
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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