Nucleoporin 50 Ran-binding domain; NUP50 acts as a cofactor for the importin-alpha:importin-beta heterodimer, which allows for transportation of many nuclear-targeted proteins through nuclear pore complexes. It is thought to function primarily at the terminal stages of nuclear protein import to coordinate import complex disassembly and importin recycling. NUP50 is composed of a N-terminal NUP50 domain which binds the C-terminus of importin-beta, a central domain which binds importin-beta, and a C-terminal RanBD which binds importin-beta through Ran-GTP. NUP50:importin-alpha then binds cargo and can stimulate nuclear import. The N-terminal domain of NUP50 is also able to displace nuclear localization signals from importin-alpha. NUP50 interacts with cyclin-dependent kinase inhibitor 1B which binds to cyclin E-CDK2 or cyclin D-CDK4 complexes and prevents its activation, thereby controling the cell cycle progression at G1. Fungal Nup2 transiently associates with nuclear pore complexes (NPCs) and when artificially tethered to DNA, can prevent the spread of transcriptional activation or repression between flanking genes, a function termed boundary activity (BA). Nup2 and the Ran guanylyl-nucleotide exchange factor, Prp20, interact at specific chromatin regions and enable the NPC to play an active role in chromatin organization. Nup60p, the nup responsible for anchoring Nup2 and the Mlp proteins to the NPC is required for Nup2-dependent BA. Nup2 contains an N-terminal Nup50 family domain and a C-terminal RanBD. Ran is a Ras-like nuclear small GTPase, which regulates receptor-mediated transport between the nucleus and the cytoplasm. RanGTP hydrolysis is stimulated by RanGAP together with the Ran-binding domain containing acessory proteins RanBP1 and RanBP2. These accessory proteins stabilize the active GTP-bound form of Ran. RabBD shares structural similarity to the PH domain, but lacks detectable sequence similarity.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907110633 389 TEVKEEDAFYSKKCKLFYKKDN-EFKEKGVGTLHLKPTA-TQKTQLLVRADTNlGNILLNVLIAPNMPCTRTGKNNVLIV 466
Cdd:cd13170     1 AGEEEEDTVFEVRAKLFKKKDDgEWKDKGVGTLRLKKHKtTGKARILVRADTL-GKILLNFLLYKGMPYSVAGKNNVFVG 79

                          90       100       110
                  ....*....|....*....|....*....|....*....
gi 1907110633 467 CVPNPPldekqptLPATMLIRVKTSEDADELHKILLEKK 505
Cdd:cd13170    80 CVPNPG-------KPVTYLLRVKTAEDADELAKALEEEK 111

Nucleoporin 50 Ran-binding domain; NUP50 acts as a cofactor for the importin-alpha:importin-beta heterodimer, which allows for transportation of many nuclear-targeted proteins through nuclear pore complexes. It is thought to function primarily at the terminal stages of nuclear protein import to coordinate import complex disassembly and importin recycling. NUP50 is composed of a N-terminal NUP50 domain which binds the C-terminus of importin-beta, a central domain which binds importin-beta, and a C-terminal RanBD which binds importin-beta through Ran-GTP. NUP50:importin-alpha then binds cargo and can stimulate nuclear import. The N-terminal domain of NUP50 is also able to displace nuclear localization signals from importin-alpha. NUP50 interacts with cyclin-dependent kinase inhibitor 1B which binds to cyclin E-CDK2 or cyclin D-CDK4 complexes and prevents its activation, thereby controling the cell cycle progression at G1. Fungal Nup2 transiently associates with nuclear pore complexes (NPCs) and when artificially tethered to DNA, can prevent the spread of transcriptional activation or repression between flanking genes, a function termed boundary activity (BA). Nup2 and the Ran guanylyl-nucleotide exchange factor, Prp20, interact at specific chromatin regions and enable the NPC to play an active role in chromatin organization. Nup60p, the nup responsible for anchoring Nup2 and the Mlp proteins to the NPC is required for Nup2-dependent BA. Nup2 contains an N-terminal Nup50 family domain and a C-terminal RanBD. Ran is a Ras-like nuclear small GTPase, which regulates receptor-mediated transport between the nucleus and the cytoplasm. RanGTP hydrolysis is stimulated by RanGAP together with the Ran-binding domain containing acessory proteins RanBP1 and RanBP2. These accessory proteins stabilize the active GTP-bound form of Ran. RabBD shares structural similarity to the PH domain, but lacks detectable sequence similarity.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907110633 389 TEVKEEDAFYSKKCKLFYKKDN-EFKEKGVGTLHLKPTA-TQKTQLLVRADTNlGNILLNVLIAPNMPCTRTGKNNVLIV 466
Cdd:cd13170     1 AGEEEEDTVFEVRAKLFKKKDDgEWKDKGVGTLRLKKHKtTGKARILVRADTL-GKILLNFLLYKGMPYSVAGKNNVFVG 79

                          90       100       110
                  ....*....|....*....|....*....|....*....
gi 1907110633 467 CVPNPPldekqptLPATMLIRVKTSEDADELHKILLEKK 505
Cdd:cd13170    80 CVPNPG-------KPVTYLLRVKTAEDADELAKALEEEK 111

Ran-binding domain; Domain of apporximately 150 residues that stabilises the GTP-bound form of Ran (the Ras-like nuclear small GTPase).

                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907110633  383 PPKVVVTEVKEEDAFYSKKCKL--FYKKDNEFKEKGVGTLH-LKPTA-TQKTQLLVRADtNLGNILLNVLIAPNMPCTR- 457
Cdd:smart00160   8 PDVEVKTGEEDEEVIFSARAKLyrFANDKKEWKERGVGDLKiLKSKDnGGKVRIVMRRD-GVLKVCANHPIFKSMTLKPl 86

                           90       100       110       120
                   ....*....|....*....|....*....|....*....|....*.
gi 1907110633  458 TGKNNVLIVcvpNPPLDEKQPTLPATMLIRVKTSEDADELHKILLE 503
Cdd:smart00160  87 AGSNRALKW---TPEDFADDIPKLVLYAVRFKTKEEADSFKNIFEE 129

Nucleoporin 50 Ran-binding domain; NUP50 acts as a cofactor for the importin-alpha:importin-beta heterodimer, which allows for transportation of many nuclear-targeted proteins through nuclear pore complexes. It is thought to function primarily at the terminal stages of nuclear protein import to coordinate import complex disassembly and importin recycling. NUP50 is composed of a N-terminal NUP50 domain which binds the C-terminus of importin-beta, a central domain which binds importin-beta, and a C-terminal RanBD which binds importin-beta through Ran-GTP. NUP50:importin-alpha then binds cargo and can stimulate nuclear import. The N-terminal domain of NUP50 is also able to displace nuclear localization signals from importin-alpha. NUP50 interacts with cyclin-dependent kinase inhibitor 1B which binds to cyclin E-CDK2 or cyclin D-CDK4 complexes and prevents its activation, thereby controling the cell cycle progression at G1. Fungal Nup2 transiently associates with nuclear pore complexes (NPCs) and when artificially tethered to DNA, can prevent the spread of transcriptional activation or repression between flanking genes, a function termed boundary activity (BA). Nup2 and the Ran guanylyl-nucleotide exchange factor, Prp20, interact at specific chromatin regions and enable the NPC to play an active role in chromatin organization. Nup60p, the nup responsible for anchoring Nup2 and the Mlp proteins to the NPC is required for Nup2-dependent BA. Nup2 contains an N-terminal Nup50 family domain and a C-terminal RanBD. Ran is a Ras-like nuclear small GTPase, which regulates receptor-mediated transport between the nucleus and the cytoplasm. RanGTP hydrolysis is stimulated by RanGAP together with the Ran-binding domain containing acessory proteins RanBP1 and RanBP2. These accessory proteins stabilize the active GTP-bound form of Ran. RabBD shares structural similarity to the PH domain, but lacks detectable sequence similarity.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907110633 389 TEVKEEDAFYSKKCKLFYKKDN-EFKEKGVGTLHLKPTA-TQKTQLLVRADTNlGNILLNVLIAPNMPCTRTGKNNVLIV 466
Cdd:cd13170     1 AGEEEEDTVFEVRAKLFKKKDDgEWKDKGVGTLRLKKHKtTGKARILVRADTL-GKILLNFLLYKGMPYSVAGKNNVFVG 79

                          90       100       110
                  ....*....|....*....|....*....|....*....
gi 1907110633 467 CVPNPPldekqptLPATMLIRVKTSEDADELHKILLEKK 505
Cdd:cd13170    80 CVPNPG-------KPVTYLLRVKTAEDADELAKALEEEK 111

Ran-binding protein 3 Ran-binding domain; RanBP3, a Ran-interacting nuclear protein, unlike the related proteins RanBP1 and RanBP2, which promote disassembly of the export complex in the cytosol, acts as a CRM1 cofactor, enhancing nuclear export signal (NES) export by stabilizing the export complex in the nucleus. CRM1/Exportin1 is responsible for exporting many proteins and ribonucleoproteins from the nucleus to the cytosol. RanBP3 also alters the cargo selectivity of CRM1, promoting recognition of the NES of HIV-1 Rev and of other cargos while deterring recognition of the import adaptor protein Snurportin1. RanBP3 contains a N-terminal nuclear localization signal (NLS), 2 FxFG motifs, and a single RanBD. RabBD shares structural similarity to the PH domain, but lacks detectable sequence similarity.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907110633 375 GEEEENDeppkvvvtevkeedaFYSKKCKL--FYKKDNEFKEKGVGTLHL---KPTATQKTQLLVRADTNLGNIlLNVLI 449
Cdd:cd13180     2 GEEGETN---------------VFQVNCKLyaFDKSKQSWKERGRGTLRLndsKSDGSGQSRIVMRTQGSLRVI-LNTKI 65

                          90       100       110       120       130
                  ....*....|....*....|....*....|....*....|....*....|....
gi 1907110633 450 APNMPCTRTGKNNVLIVCVPnpplDEKQptlPATMLIRVKtSEDADELHKILLE 503
Cdd:cd13180    66 WPGMTVEKVSEKSLRITAMD----DEGQ---VKVFLLQAS-PEDAKQLYNAIQD 111

Nucleoporin 2 Ran-binding domain; Yeast protein Nup2 transiently associates with Nuclear pore complexes (NPCs) and when artificially tethered to DNA, can prevent the spread of transcriptional activation or repression between flanking genes, a function termed boundary activity (BA). Nup2 and the Ran guanylyl-nucleotide exchange factor, Prp20, interact at specific chromatin regions and enable the NPC to play an active role in chromatin organization. Nup60p, the nup responsible for anchoring Nup2 and the Mlp proteins to the NPC is required for Nup2-dependent BA. Nup2 contains an N-terminal Nup50 family domain and a C-terminal RanBD. Ran is a Ras-like nuclear small GTPase, which regulates receptor-mediated transport between the nucleus and the cytoplasm. RanGTP hydrolysis is stimulated by RanGAP together with the Ran-binding domain containing acessory proteins RanBP1 and RanBP2. These accessory proteins stabilize the active GTP-bound form of Ran. RabBD shares structural similarity to the PH domain, but lacks detectable sequence similarity.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907110633 394 EDAFYSKKCKLFY----KKDNEFKEKGVGTLH-LKPTATQKTQLLVRADtNLGNILLNVLIAPNMPCTRTGKNNVLIVCV 468
Cdd:cd13181     6 EEVLYTKRAKLMLfdpsNTESPYTSKGVGELKlLKNKDTGKSRILVRAE-GSLRVLLNTLVLKDVKYEKMGNGSLVRVPT 84

                          90       100       110
                  ....*....|....*....|....*....|....*..
gi 1907110633 469 PNPplDEKQptlpATMLIRVKTSEDADELHKILLEKK 505
Cdd:cd13181    85 INS--DGKI----ETYVIKVKTAADGEELLKALNDAK 115

Ran-binding domain; Domain of apporximately 150 residues that stabilises the GTP-bound form of Ran (the Ras-like nuclear small GTPase).

                           10        20        30        40        50        60        70        80
                   ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907110633  383 PPKVVVTEVKEEDAFYSKKCKL--FYKKDNEFKEKGVGTLH-LKPTA-TQKTQLLVRADtNLGNILLNVLIAPNMPCTR- 457
Cdd:smart00160   8 PDVEVKTGEEDEEVIFSARAKLyrFANDKKEWKERGVGDLKiLKSKDnGGKVRIVMRRD-GVLKVCANHPIFKSMTLKPl 86

                           90       100       110       120
                   ....*....|....*....|....*....|....*....|....*.
gi 1907110633  458 TGKNNVLIVcvpNPPLDEKQPTLPATMLIRVKTSEDADELHKILLE 503
Cdd:smart00160  87 AGSNRALKW---TPEDFADDIPKLVLYAVRFKTKEEADSFKNIFEE 129

Ran-binding protein 2, repeat 1; RanBP2 (also called E3 SUMO-protein ligase RanBP2, 358 kDa nucleoporin, and nuclear pore complex (NPC) protein Nup358) is a giant nucleoporin that localizes to the cytosolic face of the NPC. RanBP2 contains a leucine-rich region, 8 zinc-finger motifs, a cyclophilin A homologous domain, and 4 RanBDs. Ran is a Ras-like nuclear small GTPase, which regulates receptor-mediated transport between the nucleus and the cytoplasm. RanGTP hydrolysis is stimulated by RanGAP together with the Ran-binding domain containing acessory proteins RanBP1 and RanBP2. These accessory proteins stabilize the active GTP-bound form of Ran. All eukaryotic cells contain RanBP1, but in vertebrates however, the main RanBP seems to be RanBP2. There is no RanBP2 ortholog in yeast. Transport complex disassembly is accomplished by a small ubiquitin-related modifier-1 (SUMO-1)-modified version of RanGAP that is bound to RanBP2. RanBP1 acts as a second line of defense against exported RanGTP#importin complexes which have escaped from dissociation by RanBP2. RanBP2 also interacts with the importin subunit beta-1. RabBD shares structural similarity to the PH domain, but lacks detectable sequence similarity. The first RanBD2 is present in this hierarchy.

                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907110633 393 EEDAFYSKKCKLFYKKDNEFKEKGVGTLHLK-PTATQKTQLLVRADTNLgNILLNVLIAPNMPCTRTGKNNVLIVCVpNP 471
Cdd:cd13169     5 NEKAVFSGDGALFEFIDGGWKERGKGELRVNlSTTTGQARLVMRARGNY-RLILNANLYPDMKLTKMGGKGVTFACV-NS 82

                          90       100       110
                  ....*....|....*....|....*....|....*.
gi 1907110633 472 PLDEKqPTLpATMLIRVKTSEDADELHKILLEKKDA 507
Cdd:cd13169    83 AADAK-DKL-STFALKFKDPQVVEEFRAAVEAHKDS 116

Ran-binding domain; RanBP1 interacts specifically with GTP-charged Ran. RanBP1 does not activate GTPase activity of Ran, but does markedly increase GTP hydrolysis by the RanGTPase-activating protein (RanGAP1). In both mammalian cells and in yeast, RanBP1 acts as a negative regulator of Regulator of chromosome condensation 1 (RCC1) by inhibiting RCC1-stimulated guanine nucleotide release from Ran. In addition to Ran, RanBP1 has been shown to interact with Exportin-1 and Importin subunit beta-1 which docks the NPC at the cytoplasmic side of the nuclear pore complex. RabBP1 contains a single RanBD. The RanBD is present in RanBD1, RanBD2, RanBD3, Nuc2, and Nuc50. Most of these proteins have a single RanBD, with the exception of RanBD2 which has 4 RanBDs. Ran is a Ras-like nuclear small GTPase, which regulates receptor-mediated transport between the nucleus and the cytoplasm. RanGTP hydrolysis is stimulated by RanGAP together with the Ran-binding domain containing acessory proteins RanBP1 and RanBP2. These accessory proteins stabilize the active GTP-bound form of Ran. The Ran-binding domain is found in multiple copies in Nuclear pore complex proteins. RabBD shares structural similarity to the PH domain, but lacks detectable sequence similarity.

                          10        20        30        40        50        60        70
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*...
gi 1907110633 381 DEPPKVVVTEVKEEDAFYSKKCKLF-YKKD---NEFKEKGVGTLH-LKPTATQKTQLLVRADTNLgNILLNVLIAPNM 453
Cdd:cd13179     9 KLPEVEVKTGEEDEEVLFKMRAKLYrFDTEndpPEWKERGTGDVKlLKHKETKKIRLLMRRDKTL-KICANHYITPEM 85