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Conserved domains on  [gi|1907150313|ref|XP_036018965|]
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OTU domain-containing protein 7B isoform X2 [Mus musculus]

Protein Classification

Graphical summary

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List of domain hits

 Name Accession Description Interval E-value
OTU_OTUD7B cd22772
OTU (ovarian tumor) domain of OTU domain-containing protein 7B; OTU domain-containing protein ...
 66-272 9.54e-159

OTU (ovarian tumor) domain of OTU domain-containing protein 7B; OTU domain-containing protein 7B (OTUD7B) is also called cellular zinc finger anti-NF-kappa-B protein, zinc finger A20 domain-containing protein 1, or zinc finger protein Cezanne. It is a deubiquitinase (DUB)/ubiquitin thioesterase (EC 3.4.19.12) that specifically targets Lys11-linked polyubiquitin. It functions as a negative regulator of the non-canonical NF-kappaB pathway by mediating the deubiquitination of TRAF3, an inhibitor of the NF-kappaB pathway, resulting in preventing TRAF3 proteolysis and over-activation of non-canonical NF-kappaB. OTUD7B also deubiquitinates ZAP70, and thus, regulates T cell receptor (TCR) signaling. It belongs to the OTU family of cysteine proteases that use a conserved cysteine, histidine, and an aspartate, as the catalytic triad. It is classified as a family C64 cysteine protease by MEROPS.


:

Pssm-ID: 438609  Cd Length: 207  Bit Score: 457.96  E-value: 9.54e-159
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907150313  66 QSMLVALEQAGRLNWWVSMDSTCQRLLPLATTGDGNCLLHAASLGMWGFHDRDLVLRKALYALMEKGVEKEALRRRWRWQ 145
Cdd:cd22772     1 QSMLVALEQAGRLNWWVSVDPTCQRLLPLATTGDGNCLLHAASLGMWGFHDRDLMLRKALYALMEKGVEKEALKRRWRWQ 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907150313 146 QTQQNKESGLVYTEDEWQKEWNELIKLASSEPRMHLGSNGASGGGVESSEEPVYESLEEFHVFVLAHVLKRPIVVVADTM 225
Cdd:cd22772    81 QTQQNKESGLVYTEDEWQKEWNELIKLASSEPRMHYGTNGANCGGVESSEEPVYESLEEFHVFVLAHVLRRPIVVVADTM 160

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*..
gi 1907150313 226 LRDSGGEAFAPIPFGGIYLPLEVPASQCHRSPLVLAYDQAHFSALVS 272
Cdd:cd22772   161 LRDSGGEAFAPIPFGGIYLPLEVPASKCHRSPLVLAYDQAHFSALVS 207
zf-A20 pfam01754
A20-like zinc finger; The A20 Zn-finger of bovine/human Rabex5/rabGEF1 is a Ubiquitin Binding ...
 706-728 1.15e-05

A20-like zinc finger; The A20 Zn-finger of bovine/human Rabex5/rabGEF1 is a Ubiquitin Binding Domain. The zinc finger mediates self-association in A20. These fingers also mediate IL-1-induced NF-kappa B activation.


:

Pssm-ID: 460313  Cd Length: 24  Bit Score: 42.49  E-value: 1.15e-05
                          10        20
                  ....*....|....*....|...
gi 1907150313 706 KCKQpNCSFYGHPETNNLCSCCY 728
Cdd:pfam01754   2 LCRN-GCGFYGSPATNGLCSKCY 23
 
Name Accession Description Interval E-value
OTU_OTUD7B cd22772
OTU (ovarian tumor) domain of OTU domain-containing protein 7B; OTU domain-containing protein ...
 66-272 9.54e-159

OTU (ovarian tumor) domain of OTU domain-containing protein 7B; OTU domain-containing protein 7B (OTUD7B) is also called cellular zinc finger anti-NF-kappa-B protein, zinc finger A20 domain-containing protein 1, or zinc finger protein Cezanne. It is a deubiquitinase (DUB)/ubiquitin thioesterase (EC 3.4.19.12) that specifically targets Lys11-linked polyubiquitin. It functions as a negative regulator of the non-canonical NF-kappaB pathway by mediating the deubiquitination of TRAF3, an inhibitor of the NF-kappaB pathway, resulting in preventing TRAF3 proteolysis and over-activation of non-canonical NF-kappaB. OTUD7B also deubiquitinates ZAP70, and thus, regulates T cell receptor (TCR) signaling. It belongs to the OTU family of cysteine proteases that use a conserved cysteine, histidine, and an aspartate, as the catalytic triad. It is classified as a family C64 cysteine protease by MEROPS.


Pssm-ID: 438609  Cd Length: 207  Bit Score: 457.96  E-value: 9.54e-159
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907150313  66 QSMLVALEQAGRLNWWVSMDSTCQRLLPLATTGDGNCLLHAASLGMWGFHDRDLVLRKALYALMEKGVEKEALRRRWRWQ 145
Cdd:cd22772     1 QSMLVALEQAGRLNWWVSVDPTCQRLLPLATTGDGNCLLHAASLGMWGFHDRDLMLRKALYALMEKGVEKEALKRRWRWQ 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907150313 146 QTQQNKESGLVYTEDEWQKEWNELIKLASSEPRMHLGSNGASGGGVESSEEPVYESLEEFHVFVLAHVLKRPIVVVADTM 225
Cdd:cd22772    81 QTQQNKESGLVYTEDEWQKEWNELIKLASSEPRMHYGTNGANCGGVESSEEPVYESLEEFHVFVLAHVLRRPIVVVADTM 160

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*..
gi 1907150313 226 LRDSGGEAFAPIPFGGIYLPLEVPASQCHRSPLVLAYDQAHFSALVS 272
Cdd:cd22772   161 LRDSGGEAFAPIPFGGIYLPLEVPASKCHRSPLVLAYDQAHFSALVS 207
OTU pfam02338
OTU-like cysteine protease; This family is comprised of a group of predicted cysteine ...
 97-267 9.23e-22

OTU-like cysteine protease; This family is comprised of a group of predicted cysteine proteases, homologous to the Ovarian Tumour (OTU) gene in Drosophila. Members include proteins from eukaryotes, viruses and pathogenic bacterium. The conserved cysteine and histidine, and possibly the aspartate, represent the catalytic residues in this putative group of proteases.


Pssm-ID: 426728 [Multi-domain]  Cd Length: 127  Bit Score: 91.36  E-value: 9.23e-22
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907150313  97 TGDGNCLLHAASLGMWGFHDRDlvlrkalyalmekgveKEALRRRWRWQQT---QQNKESGLVYTEDEWQKEWNELIKla 173
Cdd:pfam02338   1 PGDGNCLYRSISHQLWGVHDVL----------------RKMLVQELRETLAeymREHKEEFEPFLEDDETGDIIEIEQ-- 62

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907150313 174 sseprmhlgsNGASGGgvesseepvyesleEFHVFVLAHVLKRPIVVvadtmLRDSGGEAFAPIPFGGIYLPLEVPASQC 253
Cdd:pfam02338  63 ----------TGAWGG--------------EIEIFALAHILRRPIIV-----YKSEGGEELGGLKEYGIYLPLGWDPSLC 113

                         170
                  ....*....|....
gi 1907150313 254 HRSPLVLAYDQAHF 267
Cdd:pfam02338 114 LVYPRHLYYLGGHY 127
zf-A20 pfam01754
A20-like zinc finger; The A20 Zn-finger of bovine/human Rabex5/rabGEF1 is a Ubiquitin Binding ...
 706-728 1.15e-05

A20-like zinc finger; The A20 Zn-finger of bovine/human Rabex5/rabGEF1 is a Ubiquitin Binding Domain. The zinc finger mediates self-association in A20. These fingers also mediate IL-1-induced NF-kappa B activation.


Pssm-ID: 460313  Cd Length: 24  Bit Score: 42.49  E-value: 1.15e-05
                          10        20
                  ....*....|....*....|...
gi 1907150313 706 KCKQpNCSFYGHPETNNLCSCCY 728
Cdd:pfam01754   2 LCRN-GCGFYGSPATNGLCSKCY 23
ZnF_A20 smart00259
A20-like zinc fingers; A20- (an inhibitor of cell death)-like zinc fingers. The zinc finger ...
 706-728 9.03e-05

A20-like zinc fingers; A20- (an inhibitor of cell death)-like zinc fingers. The zinc finger mediates self-association in A20. These fingers also mediate IL-1-induced NF-kappaB activation.


Pssm-ID: 128555  Cd Length: 26  Bit Score: 39.88  E-value: 9.03e-05
                           10        20
                   ....*....|....*....|...
gi 1907150313  706 KCKQPNCSFYGHPETNNLCSCCY 728
Cdd:smart00259   3 KCRRPGCGFFGNPATEGLCSKCF 25
 
Name Accession Description Interval E-value
OTU_OTUD7B cd22772
OTU (ovarian tumor) domain of OTU domain-containing protein 7B; OTU domain-containing protein ...
 66-272 9.54e-159

OTU (ovarian tumor) domain of OTU domain-containing protein 7B; OTU domain-containing protein 7B (OTUD7B) is also called cellular zinc finger anti-NF-kappa-B protein, zinc finger A20 domain-containing protein 1, or zinc finger protein Cezanne. It is a deubiquitinase (DUB)/ubiquitin thioesterase (EC 3.4.19.12) that specifically targets Lys11-linked polyubiquitin. It functions as a negative regulator of the non-canonical NF-kappaB pathway by mediating the deubiquitination of TRAF3, an inhibitor of the NF-kappaB pathway, resulting in preventing TRAF3 proteolysis and over-activation of non-canonical NF-kappaB. OTUD7B also deubiquitinates ZAP70, and thus, regulates T cell receptor (TCR) signaling. It belongs to the OTU family of cysteine proteases that use a conserved cysteine, histidine, and an aspartate, as the catalytic triad. It is classified as a family C64 cysteine protease by MEROPS.


Pssm-ID: 438609  Cd Length: 207  Bit Score: 457.96  E-value: 9.54e-159
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907150313  66 QSMLVALEQAGRLNWWVSMDSTCQRLLPLATTGDGNCLLHAASLGMWGFHDRDLVLRKALYALMEKGVEKEALRRRWRWQ 145
Cdd:cd22772     1 QSMLVALEQAGRLNWWVSVDPTCQRLLPLATTGDGNCLLHAASLGMWGFHDRDLMLRKALYALMEKGVEKEALKRRWRWQ 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907150313 146 QTQQNKESGLVYTEDEWQKEWNELIKLASSEPRMHLGSNGASGGGVESSEEPVYESLEEFHVFVLAHVLKRPIVVVADTM 225
Cdd:cd22772    81 QTQQNKESGLVYTEDEWQKEWNELIKLASSEPRMHYGTNGANCGGVESSEEPVYESLEEFHVFVLAHVLRRPIVVVADTM 160

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*..
gi 1907150313 226 LRDSGGEAFAPIPFGGIYLPLEVPASQCHRSPLVLAYDQAHFSALVS 272
Cdd:cd22772   161 LRDSGGEAFAPIPFGGIYLPLEVPASKCHRSPLVLAYDQAHFSALVS 207
OTU_OTUD7 cd22768
OTU (ovarian tumor) domain of OTU domain-containing proteins 7A, 7B, and similar proteins; ...
 66-272 1.82e-139

OTU (ovarian tumor) domain of OTU domain-containing proteins 7A, 7B, and similar proteins; This subfamily consists of OTU domain-containing protein 7A (OTUD7A), OTUD7B, and similar proteins. OTUD7A and OTUD7B are deubiquitinases (DUBs)/ubiquitin thioesterases (EC 3.4.19.12) that specifically target Lys11-linked polyubiquitin. OTUD7A, also called zinc finger protein Cezanne 2, has been identified as a critical gene for brain function. It localizes to dendritic and spine compartments in cortical neurons, and its reduced levels contributed to dendritic spine and dendrite outgrowth deficits. OTUD7B, also called zinc finger protein Cezanne, functions as a negative regulator of the non-canonical NF-kappaB pathway by mediating the deubiquitination of TRAF3, an inhibitor of the NF-kappaB pathway, resulting in preventing TRAF3 proteolysis and over-activation of non-canonical NF-kappaB. OTUD7 proteins belongs to the OTU family of cysteine proteases that use a conserved cysteine, histidine, and an aspartate, as the catalytic triad. They are classified as family C64 cysteine proteases by MEROPS.


Pssm-ID: 438605  Cd Length: 208  Bit Score: 408.62  E-value: 1.82e-139
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907150313  66 QSMLVALEQAGRLNWWVSmDSTCQRLLPLATTGDGNCLLHAASLGMWGFHDRDLVLRKALYALMEKGVEKEALRRRWRWQ 145
Cdd:cd22768     1 TSTLVSLEQAGRLNWWAK-DGGCQRLLPLATTGDGNCLLHAASLGMWGFHDRLLTLRKALYETLTSSAAKEALKRRWRWQ 79

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907150313 146 QTQQNKESGLVYTEDEWQKEWNELIKLASSEPRMH--LGSNGASGGGVESSEEPVYESLEEFHVFVLAHVLKRPIVVVAD 223
Cdd:cd22768    80 QTQVNKEAGLVYSEEEWEREWKSLLKLASTEPRSQpsPSSGSELEEVIENSSDPTYESLEEIHVFVLAHVLRRPIIVVAD 159

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*....
gi 1907150313 224 TMLRDSGGEAFAPIPFGGIYLPLEVPASQCHRSPLVLAYDQAHFSALVS 272
Cdd:cd22768   160 TMLRDSNGEPLAPIPFGGIYLPLECPPSECHRSPLVLAYDAAHFSALVP 208
OTU_OTUD7A cd22773
OTU (ovarian tumor) domain of OTU domain-containing protein 7A; OTU domain-containing protein ...
 66-272 2.26e-131

OTU (ovarian tumor) domain of OTU domain-containing protein 7A; OTU domain-containing protein 7A (OTUD7A), also called zinc finger protein Cezanne 2, is a deubiquitinase (DUB)/ubiquitin thioesterase (EC 3.4.19.12) that specifically targets Lys11-linked polyubiquitin. OTUD7A has been identified as a critical gene for brain function. It localizes to dendritic and spine compartments in cortical neurons, and its reduced levels contributed to dendritic spine and dendrite outgrowth deficits. A homozygous OTUD7A missense variant located within the OTU catalytic domain is linked to early-onset epileptic encephalopathy and proteasome dysfunction. OTUD7A belongs to the OTU family of cysteine proteases that use a conserved cysteine, histidine, and an aspartate, as the catalytic triad. It is classified as a family C64 cysteine protease by MEROPS.


Pssm-ID: 438610  Cd Length: 207  Bit Score: 387.89  E-value: 2.26e-131
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907150313  66 QSMLVALEQAGRLNWWVSMDSTCQRLLPLATTGDGNCLLHAASLGMWGFHDRDLVLRKALYALMEKGVEKEALRRRWRWQ 145
Cdd:cd22773     1 QSTMVALEQAGRLNWWSTVCTSCKRLLPLATTGDGNCLLHAASLGMWGFHDRDLVLRKALYTMMKSGAEREALKRRWRWQ 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907150313 146 QTQQNKESGLVYTEDEWQKEWNELIKLASSEPRMHLGSNGASGGGVESSEEPVYESLEEFHVFVLAHVLKRPIVVVADTM 225
Cdd:cd22773    81 QTQQNKESGLVYTEEEWEREWNELLKLASSEPRTHFSKNGGTGGGVDNSEDPVYESLEEFHVFVLAHILRRPIVVVADTM 160

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*..
gi 1907150313 226 LRDSGGEAFAPIPFGGIYLPLEVPASQCHRSPLVLAYDQAHFSALVS 272
Cdd:cd22773   161 LRDSGGEAFAPIPFGGIYLPLEVPPNRCHCSPLVLAYDQAHFSALVS 207
OTU_TNFAIP3 cd22766
OTU (ovarian tumor) domain of tumor necrosis factor alpha induced protein 3; Tumor necrosis ...
 40-271 1.04e-85

OTU (ovarian tumor) domain of tumor necrosis factor alpha induced protein 3; Tumor necrosis factor (TNF) alpha-induced protein 3 (TNFAIP3) is also called OTU domain-containing protein 7C (OTUD7C), DNA-binding protein A20, or zinc finger protein A20. It is a ubiquitin-editing enzyme that contains both ubiquitin ligase and deubiquitinase (DUB)/ubiquitin thioesterase (EC 3.4.19.12) activities. It antagonizes IKK [IkappaB (inhibitor of kappaB) kinase] activation by modulating Lys63-linked polyubiquitination of cytokine-receptor-associated factors including TRAF2/6 (tumour-necrosis-factor-receptor-associated factor 2/6) and RIP1 (receptor-interacting protein 1). It can also inhibit IKK through a non-catalytic mechanism which involves polyubiquitin. In vitro, TNFAIP3 is able to deubiquitinate 'Lys-11'-, 'Lys-48'- and 'Lys-63' polyubiquitin chains. TNFAIP3 contains several A20-type zinc fingers that mediate the ubiquitin ligase activity and an OTU (ovarian tumor) domain that contains the DUB activity. It belongs to the OTU family of cysteine proteases that use a conserved cysteine, histidine, and an aspartate, as the catalytic triad. It is classified as a family C64 cysteine protease by MEROPS.


Pssm-ID: 438603  Cd Length: 220  Bit Score: 269.90  E-value: 1.04e-85
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907150313  40 MTSFKLPDLTVYKEDFRSFIERDLIEQSMLVALEQAGRLNWWvsmdSTCQRLLPLATTGDGNCLLHAASLGMWGFHDRDL 119
Cdd:cd22766     5 RYTLQLPRLCQFPPDFREFLQKALIDRNIQRTLEEAKKLNWC----REVRKLVPLKTTGDGNCLLHAVSLYMWGVQDTDL 80

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907150313 120 VLRKALYALMEKGvEKEALRRRWRWQ--QTQQNKESGLVYTEDEWQKEWNELIKLASSEPRMHLGSngasgggvesseeP 197
Cdd:cd22766    81 VLRKALYEALVET-DTRNFKLRWQRErlKSQEFVGTGLRYDTREWEEEWDNVVKMASPESKPAAGG-------------L 146

                         170       180       190       200       210       220       230
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....
gi 1907150313 198 VYESLEEFHVFVLAHVLKRPIVVVADTMLRDSGGEAFAPIPFGGIYLPLEVPASQCHRSPLVLAYDQAHFSALV 271
Cdd:cd22766   147 PYNSLEEIHIFVLANILRRPIIVIADDMLRSLEGSSLAPLNFGGIYLPLHWPPQECYKYPIVLGYDSQHFTPLV 220
OTU_C64 cd22750
OTU (ovarian tumor) domain of family C64 cysteine proteases; This group includes proteins ...
 66-271 1.09e-81

OTU (ovarian tumor) domain of family C64 cysteine proteases; This group includes proteins classified as family C64 cysteine protease by MEROPS, such as tumor necrosis factor (TNF) alpha-induced protein 3 (TNFAIP3), ZRANB1, OTU domain-containing protein 7A (OTUD7A), and OTUD7B. It also includes VCIP135. These proteins are deubiquitinases (DUBs)/ubiquitin thioesterases (EC 3.4.19.12) that mediates the deubiquitination of protein substrates. TNFAIP3 also contains ubiquitin ligase activity. It antagonizes IKK [IkappaB (inhibitor of kappaB) kinase] activation by modulating Lys63-linked polyubiquitination of cytokine-receptor-associated factors including TRAF2/6 (tumour-necrosis-factor-receptor-associated factor 2/6) and RIP1 (receptor-interacting protein 1). ZRANB1 binds, deubiquitinates, and stabilizes EZH2, which is the catalytic component of the Polycomb repressive complex 2 (PRC2) that silences gene transcription by methylating histone H3 at lysine 27 and is mutated or highly expressed in many types of cancer, including lymphoma, melanoma, prostate cancer, ovarian cancer, and breast cancer. OTUD7A has been identified as a critical gene for brain function, while OTUD7B functions as a negative regulator of the non-canonical NF-kappaB pathway by mediating the deubiquitination of TRAF3, an inhibitor of the NF-kappaB pathway, resulting in preventing TRAF3 proteolysis and over-activation of non-canonical NF-kappaB. VCIP135 controls Golgi membrane dynamics in the cell cycle and is required for Golgi and ER assembly. This group belongs to the OTU family of cysteine proteases that use a conserved cysteine and histidine, and in most cases an aspartate, as the catalytic triad.


Pssm-ID: 438587  Cd Length: 185  Bit Score: 258.04  E-value: 1.09e-81
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907150313  66 QSMLVALEQAGRLNWwvsmDSTCQRLLPLATTGDGNCLLHAASLGMWGFHDRDLVLRKALYALMEKGVEKeALRRRWRWQ 145
Cdd:cd22750     1 RDLKDDLESEKVINW----CRGVSRLVPLHTRGDGNCLLHAVSLALWGVEDRDLLLRSALHETLQNDQER-RFRARWRRQ 75

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907150313 146 QTQQNKESGLVYTEDEWQKEWNELIKLASSEPRMHLgsngasgggvesseepVYESLEEFHVFVLAHVLKRPIVVVADTM 225
Cdd:cd22750    76 QLKSGQELGLSLDEEALQAEWEEILKAAETPTVPAG----------------PGSYLEEIHIFVLANVLRRPIIVLADDS 139

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|....*.
gi 1907150313 226 LRDSGGEAFAPIPFGGIYLPLEVPASQCHRSPLVLAYDQAHFSALV 271
Cdd:cd22750   140 ARSLEGSALQDNGMSGIYLPLLWPPSECSRSPLALGYSNGHFSPLV 185
OTU_ZRANB1 cd22767
OTU (ovarian tumor) domain of Ubiquitin thioesterase ZRANB1 and similar proteins; ZRANB1 is ...
 72-272 3.89e-40

OTU (ovarian tumor) domain of Ubiquitin thioesterase ZRANB1 and similar proteins; ZRANB1 is also called TRAF-binding domain-containing protein, Trabid, or zinc finger Ran-binding domain-containing protein 1. It is a deubiquitinase (DUB)/ubiquitin thioesterase (EC 3.4.19.12) that specifically hydrolyzes 'Lys-29'-linked and 'Lys-33'-linked diubiquitin; it also cleaves 'Lys-63'-linked chains with less efficiency. ZRANB1 binds, deubiquitinates, and stabilizes EZH2, which is the catalytic component of the Polycomb repressive complex 2 (PRC2) that silences gene transcription by methylating histone H3 at lysine 27 and is mutated or highly expressed in many types of cancer, including lymphoma, melanoma, prostate cancer, ovarian cancer, and breast cancer. Drosophila Trabid interacts with TGF-beta Activating Kinase 1 (TAK1), which triggers both immunity and apoptosis, resulting in reduced immune signaling output and K63-linked ubiquitination. ZRANB1 belongs to the OTU family of cysteine proteases that use a conserved cysteine and histidine, and in most cases an aspartate, as the catalytic triad. ZRANB1 does not contain the conserved aspartate, and uses cysteine and histidine as a catalytic dyad. It is classified as a family C64 cysteine protease by MEROPS.


Pssm-ID: 438604  Cd Length: 185  Bit Score: 145.90  E-value: 3.89e-40
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907150313  72 LEQAGR-LNWWVSMDSTCQ-RLLPLATTGDGNCLLHAASLGMWGFHDRDLVLRKALYALMEKGVEKeaLRRRWRWQQTQQ 149
Cdd:cd22767     7 LEEESPiINWSLELTDRLGsRLYALWNRTAGDCLLDSVLQATWGVFDRDNVLRRALADSLHDCAHW--FYSRWKEYESWQ 84

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907150313 150 NKESGLVYTEDEWQKEWNELIKLASSePRMhlgsngasgggvesseepvyeSLEEFHVFVLAHVLKRPIVVVADTMLRDS 229
Cdd:cd22767    85 AQSLGYSLEEEQWQKDWAFLLSLASQ-PGA---------------------SLEQTHIFALAHILRRPIIVYGVKYVKSF 142

                         170       180       190       200
                  ....*....|....*....|....*....|....*....|...
gi 1907150313 230 GGEAFAPIPFGGIYLPLEVPASQCHRSPLVLAYDQAHFSALVS 272
Cdd:cd22767   143 RGETLGYARFQGVYLPLLWEQSFCWKSPIALGYTRGHFSALVP 185
OTU pfam02338
OTU-like cysteine protease; This family is comprised of a group of predicted cysteine ...
 97-267 9.23e-22

OTU-like cysteine protease; This family is comprised of a group of predicted cysteine proteases, homologous to the Ovarian Tumour (OTU) gene in Drosophila. Members include proteins from eukaryotes, viruses and pathogenic bacterium. The conserved cysteine and histidine, and possibly the aspartate, represent the catalytic residues in this putative group of proteases.


Pssm-ID: 426728 [Multi-domain]  Cd Length: 127  Bit Score: 91.36  E-value: 9.23e-22
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907150313  97 TGDGNCLLHAASLGMWGFHDRDlvlrkalyalmekgveKEALRRRWRWQQT---QQNKESGLVYTEDEWQKEWNELIKla 173
Cdd:pfam02338   1 PGDGNCLYRSISHQLWGVHDVL----------------RKMLVQELRETLAeymREHKEEFEPFLEDDETGDIIEIEQ-- 62

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907150313 174 sseprmhlgsNGASGGgvesseepvyesleEFHVFVLAHVLKRPIVVvadtmLRDSGGEAFAPIPFGGIYLPLEVPASQC 253
Cdd:pfam02338  63 ----------TGAWGG--------------EIEIFALAHILRRPIIV-----YKSEGGEELGGLKEYGIYLPLGWDPSLC 113

                         170
                  ....*....|....
gi 1907150313 254 HRSPLVLAYDQAHF 267
Cdd:pfam02338 114 LVYPRHLYYLGGHY 127
OTU cd22744
OTU (ovarian tumor) domain family; The OTU family of cysteine proteases use a conserved ...
 96-270 3.10e-09

OTU (ovarian tumor) domain family; The OTU family of cysteine proteases use a conserved cysteine and histidine, and in most cases an aspartate, as the catalytic triad. OTU domains typically function as deubiquitinases (DUBs)/ubiquitin thiolesterases (EC 3.4.19.12) that catalyze the thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin, a small regulatory protein that can be conjugated to a large range of target proteins. Protein ubiquitination is a post-translational modification of mostly Lys residues that regulates many cellular processes, including protein degradation, intracellular trafficking, cell signaling, autophagy, transcription, translation, and the DNA damage response. These DUBs may play important regulatory roles at the level of protein turnover by preventing degradation.


Pssm-ID: 438581 [Multi-domain]  Cd Length: 128  Bit Score: 55.52  E-value: 3.10e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907150313  96 TTGDGNCLLHAASLGMWGFHDRDLVLRKALYALMEKgvekealrrrwrwqqtQQNKESGLVYTEDEWQKEWNELIKlass 175
Cdd:cd22744     5 VPGDGNCLFRALAHALYGDQESHRELRQEVVDYLRE----------------NPDLYEPAELADEDDGEDFDEYLQ---- 64

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907150313 176 eprmHLGSNGASGGGVEsseepvyesleefhVFVLAHVLKRPIVVVadtmlrDSGGEAFAPIPFGgiylplevPASQCHR 255
Cdd:cd22744    65 ----RMRKPGTWGGELE--------------LQALANALNVPIVVY------SEDGGFLPVSVFG--------PGPGPSG 112

                         170
                  ....*....|....*.
gi 1907150313 256 SPLVLAYDQA-HFSAL 270
Cdd:cd22744   113 RPIHLLYTGGnHYDAL 128
OTU_VCIP135 cd22769
OTU (ovarian tumor) domain of deubiquitinating protein VCIP135; Deubiquitinating protein ...
 89-271 4.51e-09

OTU (ovarian tumor) domain of deubiquitinating protein VCIP135; Deubiquitinating protein VCIP135 is also called valosin-containing protein p97/p47 complex-interacting protein 1, valosin-containing protein p97/p47 complex-interacting protein p135, or VCP/p47 complex-interacting 135-kDa protein. It is a deubiquitinase (DUB)/ubiquitin thioesterase (EC 3.4.19.12) that hydrolyzes 'Lys-11'- and 'Lys-48'-linked polyubiquitin chains. It is necessary for VCP-mediated reassembly of Golgi stacks after mitosis, and may play a role in VCP-mediated formation of transitional endoplasmic reticulum (tER). VCIP135 controls Golgi membrane dynamics in the cell cycle and is required for Golgi and ER assembly. It belongs to the OTU family of cysteine proteases that use a conserved cysteine and histidine, and in most cases an aspartate, as the catalytic triad. Not all members of this subfamily contain the active site. It is closely related to proteins classified as family C64 cysteine protease by MEROPS, such as TNFAIP3, ZRANB1, and OTUD7A/B.


Pssm-ID: 438606  Cd Length: 197  Bit Score: 56.92  E-value: 4.51e-09
                          10        20        30        40        50        60        70        80
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907150313  89 QRLLPLATTGDGNCLLHAASlgmwgfhdRDLVLRKALY-ALmekgveKEALRRRWRWQQTQ-QNKESGLVYTEdewqkEW 166
Cdd:cd22769    43 ERLIPIHADGDGHCLVHAVS--------RALVGRELFWhAL------RENLKQHFKENLDQyKALFQDFIDDS-----EW 103

                          90       100       110       120       130       140       150       160
                  ....*....|....*....|....*....|....*....|....*....|....*....|....*....|....*....|
gi 1907150313 167 NELIklASSEPRMHLGSNGASGggvesseepvyesLEEFHVFVLAHVLKRPIVVVaDTM--LRDSGGeafapipFGGIYL 244
Cdd:cd22769   104 PDII--AECDPDFVPPEGEPLG-------------LRNIHIFGLANVLKRPIILL-DSLsgMQSSGD-------YSAIFL 160

                         170       180       190
                  ....*....|....*....|....*....|....*.
gi 1907150313 245 PLEVPASQCH------RSPLVLAYDQA---HFSALV 271
Cdd:cd22769   161 PGLVPPEKCRgkdgllNKPICIAWSSSgrnHFIPLV 196
zf-A20 pfam01754
A20-like zinc finger; The A20 Zn-finger of bovine/human Rabex5/rabGEF1 is a Ubiquitin Binding ...
 706-728 1.15e-05

A20-like zinc finger; The A20 Zn-finger of bovine/human Rabex5/rabGEF1 is a Ubiquitin Binding Domain. The zinc finger mediates self-association in A20. These fingers also mediate IL-1-induced NF-kappa B activation.


Pssm-ID: 460313  Cd Length: 24  Bit Score: 42.49  E-value: 1.15e-05
                          10        20
                  ....*....|....*....|...
gi 1907150313 706 KCKQpNCSFYGHPETNNLCSCCY 728
Cdd:pfam01754   2 LCRN-GCGFYGSPATNGLCSKCY 23
ZnF_A20 smart00259
A20-like zinc fingers; A20- (an inhibitor of cell death)-like zinc fingers. The zinc finger ...
 706-728 9.03e-05

A20-like zinc fingers; A20- (an inhibitor of cell death)-like zinc fingers. The zinc finger mediates self-association in A20. These fingers also mediate IL-1-induced NF-kappaB activation.


Pssm-ID: 128555  Cd Length: 26  Bit Score: 39.88  E-value: 9.03e-05
                           10        20
                   ....*....|....*....|...
gi 1907150313  706 KCKQPNCSFYGHPETNNLCSCCY 728
Cdd:smart00259   3 KCRRPGCGFFGNPATEGLCSKCF 25
OTU_VRTN cd22791
OTU (ovarian tumor) domain of vertnin and similar proteins; Vertnin (VRTN) is an OTU ...
 91-113 1.08e-04

OTU (ovarian tumor) domain of vertnin and similar proteins; Vertnin (VRTN) is an OTU domain-containing protein that is required for the development of thoracic vertebrae in mammals. OTU domains typically function as deubiquitinases (DUBs)/ubiquitin thiolesterases (EC 3.4.19.12) that catalyze the thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin, a small regulatory protein that can be conjugated to a large range of target proteins. Protein ubiquitination is a post-translational modification of mostly Lys residues that regulates many cellular processes, including protein degradation, intracellular trafficking, cell signaling, autophagy, transcription, translation, and the DNA damage response. These DUBs may play important regulatory roles at the level of protein turnover by preventing degradation. Vertnin and some subfamily members do not possess the conserved catalytic residues and may not have DUB activity. VRTN gene is associated with variations in vertebral number.


Pssm-ID: 438612  Cd Length: 137  Bit Score: 42.59  E-value: 1.08e-04
                          10        20
                  ....*....|....*....|...
gi 1907150313  91 LLPLATTGDGNCLLHAASLGMWG 113
Cdd:cd22791     1 LEPLRVTGDGNCLFRAASLLLFG 23
 
Blast search parameters
Data Source: Precalculated data, version = cdd.v.3.21
Preset Options:Database: CDSEARCH/cdd   Low complexity filter: no  Composition Based Adjustment: yes   E-value threshold: 0.01

References:

  • Wang J et al. (2023), "The conserved domain database in 2023", Nucleic Acids Res.51(D)384-8.
  • Lu S et al. (2020), "The conserved domain database in 2020", Nucleic Acids Res.48(D)265-8.
  • Marchler-Bauer A et al. (2017), "CDD/SPARCLE: functional classification of proteins via subfamily domain architectures.", Nucleic Acids Res.45(D)200-3.
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