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Biotechnology Information

6X6T: Cryo-EM structure of an Escherichia coli coupled transcription-translation complex B1 (TTC-B1) containing an mRNA with a 24 nt long spacer, transcription factors NusA and NusG, and fMet-tRNAs at P-site and E-site

PDB ID:

6X6T Download

MMDB ID:

255175

PDB Deposition Date:

2020/5/29

Updated in MMDB:

2024/12

Experimental Method:

electron microscopy

Resolution:

3.2 Å

Source Organism:

synthetic construct▼

Taxonomy	Molecule
synthetic construct	5, 6, 7
Escherichia coli	0, 1, 2, 3, 4, 9, A, AA, AB, AC, AD, AE, AF, AG, B, C, D, E, F, G, H , I, J, K, L, M, N, O, P, Q, R, S, T, U, V, W, X, Y, Z, a, b, c, d, e, f, g, h, i, j, k, l, m, n , o, p, q, r, s, t, u, v, w, x, y, z

Similar Structures:

Biological Unit for 6X6T: 68-meric; determined by author

Molecular Components in 6X6T
Label	Count	Molecule
Proteins (60 molecules)
0	1	50S Ribosomal Protein L21
1	1	50S Ribosomal Protein L22
2	1	50S Ribosomal Protein L23
3	1	50S Ribosomal Protein L24
4	1	50S Ribosomal Protein L25
9	1	50S Ribosomal Protein L10
AA	1	DNA-directed RNA Polymerase Subunit Beta
AB	1	Transcription Termination/antitermination Protein Nusg
AC AD	2	DNA-directed RNA Polymerase Subunit Alpha
AE	1	DNA-directed RNA Polymerase Subunit Beta'
AF	1	DNA-directed RNA Polymerase Subunit Omega
AG	1	Transcription Termination/antitermination Protein Nusa
C	1	30S Ribosomal Protein S18
E	1	30S Ribosomal Protein S20
F	1	30S Ribosomal Protein S21
G	1	30S Ribosomal Protein S2
H	1	30S Ribosomal Protein S1
I	1	30S Ribosomal Protein S3
J	1	30S Ribosomal Protein S4
K	1	30S Ribosomal Protein S5
L	1	30S Ribosomal Protein S6
M	1	30S Ribosomal Protein S7
N	1	30S Ribosomal Protein S8
O	1	30S Ribosomal Protein S9
P	1	30S Ribosomal Protein S10
Q	1	30S Ribosomal Protein S11
R	1	30S Ribosomal Protein S12
S	1	30S Ribosomal Protein S14
T	1	30S Ribosomal Protein S15
U	1	30S Ribosomal Protein S16
V	1	30S Ribosomal Protein S17
W	1	30S Ribosomal Protein S19
X	1	30S Ribosomal Protein S13
Y	1	50S Ribosomal Protein L11
Z	1	50S Ribosomal Protein L7/l12
b	1	50S Ribosomal Protein L27
c	1	50S Ribosomal Protein L28
e	1	50S Ribosomal Protein L29
f	1	50S Ribosomal Protein L30
g	1	50S Ribosomal Protein L31
h	1	50S Ribosomal Protein L2
i	1	50S Ribosomal Protein L32
j	1	50S Ribosomal Protein L3
k	1	50S Ribosomal Protein L33
l	1	50S Ribosomal Protein L4
m	1	50S Ribosomal Protein L34
n	1	50S Ribosomal Protein L5
o	1	50S Ribosomal Protein L35
p	1	50S Ribosomal Protein L6
q	1	50S Ribosomal Protein L36
r	1	50S Ribosomal Protein L9
s	1	50S Ribosomal Protein L13
t	1	50S Ribosomal Protein L14
u	1	50S Ribosomal Protein L15
v	1	50S Ribosomal Protein L16
w	1	50S Ribosomal Protein L17
x	1	50S Ribosomal Protein L18
y	1	50S Ribosomal Protein L19
z	1	50S Ribosomal Protein L20
Nucleotides(8 molecules)
5	1	NT DNA
6	1	T DNA
7	1	mRNA With 24 NT Long Spacer
A B	2	E-site and P-site tRNA (Fmet)
D	1	16S rRNA
a	1	23S rRNA
d	1	5S rRNA
Chemicals and Non-standard biopolymers (3 molecules)
1	1	MAGNESIUM ION
2	2	ZINC ION

* Click molecule labels to explore molecular sequence information.

Citing MMDB

Madej T, Lanczycki CJ, Zhang D, Thiessen PA, Geer RC, Marchler-Bauer A, Bryant SH. " MMDB and VAST+: tracking structural similarities between macromolecular complexes. Nucleic Acids Res. 2014 Jan; 42(Database issue):D297-303