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8URX: Escherichia coli transcription-translation coupled complex class B (TTC-B) containing RfaH bound to ops signal, mRNA with a 30 nt long spacer, and fMet-tRNAs in E-site and P-site of the ribosome

PDB ID:

8URX Download

MMDB ID:

247668

PDB Deposition Date:

2023/10/27

Updated in MMDB:

2024/12

Experimental Method:

electron microscopy

Resolution:

6.6 Å

Source Organism:

synthetic construct▼

Taxonomy	Molecule
synthetic construct	5, 6, 7
Escherichia coli	0, 1, 2, 3, 4, 9, A, AA, AB, AC, AD, AE, AF, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T , U, V, W, X, Y, Z, a, b, c, d, e, f, g, h, i, j, k, l, m, n, o, p, q, r, s, t, u, v, w, x, y, z

Similar Structures:

Biological Unit for 8URX: 67-meric; determined by author

Molecular Components in 8URX
Label	Count	Molecule
Proteins (59 molecules)
0	1	Ribosomal Protein L21
1	1	50S Ribosomal Protein L22
2	1	50S Ribosomal Protein L23
3	1	50S Ribosomal Protein L24
4	1	50S Ribosomal Protein L25
9	1	50S Ribosomal Protein L10
AA	1	DNA-directed RNA Polymerase Subunit Beta
AB	1	Transcription Antitermination Protein Rfah
AC AD	2	DNA-directed RNA Polymerase Subunit Alpha
AE	1	DNA-directed RNA Polymerase Subunit Beta'
AF	1	DNA-directed RNA Polymerase Subunit Omega
C	1	30S Ribosomal Protein S18
E	1	30S Ribosomal Protein S20
F	1	30S Ribosomal Protein S21
G	1	30S Ribosomal Protein S2
H	1	30S Ribosomal Protein S1
I	1	30S Ribosomal Protein S3
J	1	30S Ribosomal Protein S4
K	1	30S Ribosomal Protein S5
L	1	30S Ribosomal Protein S6
M	1	30S Ribosomal Protein S7
N	1	30S Ribosomal Protein S8
O	1	30S Ribosomal Protein S9
P	1	30S Ribosomal Protein S10
Q	1	30S Ribosomal Protein S11
R	1	30S Ribosomal Protein S12
S	1	30S Ribosomal Protein S14
T	1	30S Ribosomal Protein S15
U	1	30S Ribosomal Protein S16
V	1	30S Ribosomal Protein S17
W	1	30S Ribosomal Protein S19
X	1	30S Ribosomal Protein S13
Y	1	50S Ribosomal Protein L11
Z	1	50S Ribosomal Protein L7/l12
b	1	50S Ribosomal Protein L27
c	1	50S Ribosomal Protein L28
e	1	50S Ribosomal Protein L29
f	1	50S Ribosomal Protein L30
g	1	50S Ribosomal Protein L31
h	1	50S Ribosomal Protein L2
i	1	50S Ribosomal Protein L32
j	1	50S Ribosomal Protein L3
k	1	50S Ribosomal Protein L33
l	1	50S Ribosomal Protein L4
m	1	50S Ribosomal Protein L34
n	1	50S Ribosomal Protein L5
o	1	50S Ribosomal Protein L35
p	1	50S Ribosomal Protein L6
q	1	50S Ribosomal Protein L36
r	1	50S Ribosomal Protein L9
s	1	50S Ribosomal Protein L13
t	1	50S Ribosomal Protein L14
u	1	50S Ribosomal Protein L15
v	1	50S Ribosomal Protein L16
w	1	50S Ribosomal Protein L17
x	1	50S Ribosomal Protein L18
y	1	50S Ribosomal Protein L19
z	1	50S Ribosomal Protein L20
Nucleotides(8 molecules)
5	1	NT DNA OPS
6	1	T DNA OPS
7	1	mRNA With 30 NT Long Spacer
A B	2	E-site and P-site Fmet-trna
D	1	16S rRNA
a	1	23S rRNA
d	1	5S rRNA
Chemicals and Non-standard biopolymers (3 molecules)
1	1	MAGNESIUM ION
2	2	ZINC ION

* Click molecule labels to explore molecular sequence information.

Citing MMDB

Madej T, Lanczycki CJ, Zhang D, Thiessen PA, Geer RC, Marchler-Bauer A, Bryant SH. " MMDB and VAST+: tracking structural similarities between macromolecular complexes. Nucleic Acids Res. 2014 Jan; 42(Database issue):D297-303