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8URY: Escherichia coli transcription-translation coupled complex class B (TTC-B) containing RfaH bound to ops signal, NusA, mRNA with a 30 nt long spacer, and fMet-tRNA in E-site and P-site of the ribosome

PDB ID:

8URY Download

MMDB ID:

248242

PDB Deposition Date:

2023/10/27

Updated in MMDB:

2024/12

Experimental Method:

electron microscopy

Resolution:

3.1 Å

Source Organism:

synthetic construct▼

Taxonomy	Molecule
synthetic construct	5, 6, 7
Escherichia coli	0, 1, 2, 3, 4, 9, A, AA, AB, AC, AD, AE, AF, AG, B, C, D, E, F, G, H , I, J, K, L, M, N, O, P, Q, R, S, T, U, V, W, X, Y, Z, a, b, c, d, e, f, g, h, i, j, k, l, m, n , o, p, q, r, s, t, u, v, w, x, y, z

Similar Structures:

Biological Unit for 8URY: 68-meric; determined by author

Molecular Components in 8URY
Label	Count	Molecule
Proteins (60 molecules)
0	1	50S Ribosomal Protein L21
1	1	50S Ribosomal Protein L22
2	1	50S Ribosomal Protein L23
3	1	50S Ribosomal Protein L24
4	1	Large Ribosomal Subunit Protein Bl25
9	1	50S Ribosomal Protein L10
AA	1	DNA-directed RNA Polymerase Subunit Beta
AB	1	Transcription Antitermination Protein Rfah
AC AD	2	DNA-directed RNA Polymerase Subunit Alpha
AE	1	DNA-directed RNA Polymerase Subunit Beta'
AF	1	DNA-directed RNA Polymerase Subunit Omega
AG	1	Transcription Termination/antitermination Protein Nusa
C	1	30S Ribosomal Protein S18
E	1	30S Ribosomal Protein S20
F	1	30S Ribosomal Protein S21
G	1	30S Ribosomal Protein S2
H	1	Small Ribosomal Subunit Protein BS1
I	1	30S Ribosomal Protein S3
J	1	30S Ribosomal Protein S4
K	1	30S Ribosomal Protein S5
L	1	30S Ribosomal Protein S6
M	1	30S Ribosomal Protein S7
N	1	30S Ribosomal Protein S8
O	1	30S Ribosomal Protein S9
P	1	Small Ribosomal Subunit Protein Us10
Q	1	30S Ribosomal Protein S11
R	1	30S Ribosomal Protein S12
S	1	30S Ribosomal Protein S14
T	1	30S Ribosomal Protein S15
U	1	30S Ribosomal Protein S16
V	1	30S Ribosomal Protein S17
W	1	30S Ribosomal Protein S19
X	1	30S Ribosomal Protein S13
Y	1	50S Ribosomal Protein L11
Z	1	50S Ribosomal Protein L7/l12
b	1	Large Ribosomal Subunit Protein Bl27
c	1	50S Ribosomal Protein L28
e	1	Large Ribosomal Subunit Protein Ul29
f	1	50S Ribosomal Protein L30
g	1	Large Ribosomal Subunit Protein Bl31
h	1	50S Ribosomal Protein L2
i	1	50S Ribosomal Protein L32
j	1	50S Ribosomal Protein L3
k	1	50S Ribosomal Protein L33
l	1	50S Ribosomal Protein L4
m	1	50S Ribosomal Protein L34
n	1	50S Ribosomal Protein L5
o	1	Large Ribosomal Subunit Protein Bl35
p	1	50S Ribosomal Protein L6
q	1	50S Ribosomal Protein L36
r	1	50S Ribosomal Protein L9
s	1	Large Ribosomal Subunit Protein Ul13
t	1	50S Ribosomal Protein L14
u	1	50S Ribosomal Protein L15
v	1	50S Ribosomal Protein L16
w	1	50S Ribosomal Protein L17
x	1	50S Ribosomal Protein L18
y	1	Large Ribosomal Subunit Protein Bl19
z	1	50S Ribosomal Protein L20
Nucleotides(8 molecules)
5	1	NT DNA OPS
6	1	T DNA
7	1	mRNA With 30 NT Long Spacer
A B	2	E-site and P-site Fmet-trna
D	1	16S rRNA
a	1	23S rRNA
d	1	5S rRNA
Chemicals and Non-standard biopolymers (3 molecules)
1	1	MAGNESIUM ION
2	2	ZINC ION

* Click molecule labels to explore molecular sequence information.

Citing MMDB

Madej T, Lanczycki CJ, Zhang D, Thiessen PA, Geer RC, Marchler-Bauer A, Bryant SH. " MMDB and VAST+: tracking structural similarities between macromolecular complexes. Nucleic Acids Res. 2014 Jan; 42(Database issue):D297-303