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Accession: PRJNA193494 ID: 193494

Non-Hybrid, Finished Microbial Genome Assemblies from Long-Read SMRT Sequencing Data

The datasets were collected for demonstrating a hierarchical genome assembly process (HGAP) to generate high quality de novo microbial genome assemblies. The new method utilizes a single, long insert shotgun DNA library in conjunction with Single Molecule, Real Time (SMRT) DNA sequencing and obviates the need for additional sample preparation and sequencing data sets required for previously described hybrid assembly strategies. We demonstrate the efficient de novo assembly from genomic DNA to a finished genome sequence for several microorganisms.
AccessionPRJNA193494
TypeUmbrella Comparative genomics project (Subtype:Comparative genomics)
SubmissionRegistration date: 20-Mar-2013
Pacific Biosciences
Relevanceassembly algorithm
Project Data:
Resource NameNumber
of Links
Sequence data
Nucleotide (total)8
WGS master2
Genomic DNA1
SRA Experiments4
Protein Sequences3056
Other datasets
BioSample4
Assembly3
SRA Data Details
ParameterValue
Data volume, Gbases14
Data volume, Mbytes16904
This project encompasses the following 3 sub-projects:
Project TypeNumber of Projects
Genome sequencing
Highest level of assembly :
Chromosomes
Contigs
Total

1
2
3
BioProject
accession
Assembly
level
OrganismTitle
PRJNA188384 ContigsEscherichia coli str. K-12 substr. MG1655Escherichia coli str. K-12 substr. MG1655 Genome sequencing and assembly (Pacific Biosciences)
PRJNA196343 ChromosomesMeiothermus ruber DSM 1279Meiothermus ruber DSM 1279 Genome sequencing (Pacific Biosciences)
PRJNA196344 ContigsPedobacter heparinus DSM 2366Pedobacter heparinus DSM 2366 Genome sequencing and assembly (Pacific Biosciences)

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