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Genome Information for Staphylococcus epidermidis
Purpose: To compare the transcriptome changes in phoU1(serp0956) or phoU2(serp0316)deletion strain with the parent strain SE1457 in log-phase (6 h)
Methods: Total RNA was isolated and sequenced from the phoU1 deletion strain, phoU2 deletion strain and the parent strain SE1457 in log-phase (6 h) in triplicate using an illumina high-seq 2500. Raw data was analyzed using TopHat. Genes were considered changed which performed fold-change>=1.5 and FDR<=0.05. Changed genes were then filtered to reveal the downstream targets of phoU1 or phoU2 in log-phase (6 h).
Results: The expression of 23 genes were significantly decreased and 69 genes was significantly increased in expression level due to the loss of phoU1 expression.The expression of 474 genes were significantly decreased and 471 genes were significantly increased in expression level due to the loss of phoU2 expression.
Overall design: Total three biological samples are analyzied, each of them had triplication.The three samples are parent strain SE1457,the phoU1 deletion strain and phoU2 deletion strain.
| Accession | PRJNA381636; GEO: GSE97400 |
| Data Type | Transcriptome or Gene expression |
| Scope | Multiisolate |
| Organism | Staphylococcus epidermidis[Taxonomy ID: 1282] Bacteria; Bacillati; Bacillota; Bacilli; Bacillales; Staphylococcaceae; Staphylococcus; Staphylococcus epidermidis |
| Publications | Wang X et al., "PhoU2 but Not PhoU1 as an Important Regulator of Biofilm Formation and Tolerance to Multiple Stresses by Participating in Various Fundamental Metabolic Processes in Staphylococcus epidermidis.", J Bacteriol, 2017 Dec 15;199(24) |
| Submission | Registration date: 4-Apr-2017
MOH&MOE Key Laboratory of Medical Molecular Virology, Shanghai Medical College, Fudan University |
| Relevance | Medical |
Project Data:
| Resource Name | Number
of Links |
|---|
| Sequence data |
| SRA Experiments | 9 |
| Publications |
| PubMed | 1 |
| PMC | 1 |
| Other datasets |
| BioSample | 9 |
| GEO DataSets | 1 |