From Brachypodium grown in natural soil under controlled lab conditions, we enriched the root-associated microbes, utilizing carbon compounds prevalent in Brachypodium root exudates.
More...From Brachypodium grown in natural soil under controlled lab conditions, we enriched the root-associated microbes, utilizing carbon compounds prevalent in Brachypodium root exudates. By transferring the enrichments every 3 or 7 days for 9 generations, we developed both fast and slow-growing microbial communities. 16S rRNA amplicon analysis revealed that both inoculum and carbon substrates significantly influence microbial community composition. For example, 1/10 R2A preferentially enriched Amplicon Sequence Variants (ASVs) from slow growing taxa vital to plant including Acidobacteria and Verrucomicrobia. Network analysis revealed that although fast and slow growing microbial consortia have distinct key taxa, the key hubs (keystone taxa) for both belong to genera with plant growth promoting (PGP) traits. This suggests that PGP bacteria might play a central role in controlling the microbial networks among rhizospheric microbiomes. Based on the stability and richness results from different transfers, most carbon substrates lead to microbial consortia with reduced complexity and high stability after a few transfers. The stability tests of the derived microbial consortia also showed high stability, reproducibility, and revivability of the constructed microbial consortia. Our study represents a significant step towards understanding and harnessing the potential of rhizosphere microbiomes, with implications for sustainable agriculture and environmental management.
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