Protocols: Samples were harvested from all replicates as follows: 20 mL bacterial broth were decanted (heterotrophic) or drawn via cannula and syringe (autotrophic), aliquoted into 2 mL-cryo tubes and immediately centrifuged for 30 s at 16 900 x g and room temperature. The supernatants were decanted, the cell pellets instantly shock-frozen in liquid nitrogen and stored at - 80 °C until further analysis. Organism used - Oligotropha carboxidovorans OM5 genome sequence including the plasmids pHCG3 and pOC167(accession numbers CP002826.1; CP002827.1 and CP002828.1 ) (Volland et al., 2011). Volland, S., Rachinger, M., Strittmatter, A., Daniel, R., Gottschalk, G., & Meyer, O. (2011). Complete genome sequences of the chemolithoautotrophic Oligotropha carboxidovorans strains OM4 and OM5. Journal of Bacteriology, 193(18), 5043. https://doi.org/10.1128/JB.05619-11 The cells grown aerobically in OC minimal medium and acetate or syngas as sole carbon and energy source. Total RNA was isolated from four biological replicates using Zymo Quick-RNA Miniprep Plus kit with bead beating and DNase-treatment (Zymo Research, Freiburg, Germany). Initially RNA quality was checked by Trinean Xpose (Gentbrugge,Belgium) and Agilent RNA Nano 6000 kit on Agilent 2100 Bioanalyzer (Agilent Technologies, Böblingen, Germany). Samples contaminated with DNA were treated with DNase again, cleaned as described above and rechecked by Xpose and Agilent Bioanalyzer. Finally RNA was free of DNA with an RNA Integrity Number (RIN) > 9 and rRNA Ratio [23s / 16s] > 1.5. Ribo-Zero rRNA Removal Kit (Bacteria) from Illumina (San Diego, CA, USA) was used to remove the ribosomal RNA molecules from the isolated total RNA. Removal of rRNA was checked by Agilent RNA Pico 6000 kit on Agilent 2100 Bioanalyzer (Agilent Technologies, Böblingen, Germany). RNA was free of detectable rRNA. TruSeq Stranded mRNA Library Prep Kit from Illumina (San Diego, CA, USA) was used to prepare cDNA libraries.