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Purified DNA was randomly sheared using the Hydroshear machine (GeneMachines, San Carlos, CA). The ends of the sheared DNA were repaired, and size selected by electrophoresis. The eluted DNA fragments were cloned into the pSMART vector (Lucigen, Middleton, WI). Purified plasmids were sequenced. The vector sequence was removed prior to submission.
Nucleotide
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