Pseudomonas aeruginosa T3SS screen

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Result Filters

Identifiers: BioSample: SAMN29146374; Sample name: Pseudomonas aeruginosa strain PAK PexoS-mNeomGreen library; SRA: SRS13424383

Organism: Pseudomonas aeruginosa PAK
cellular organisms; Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas; Pseudomonas aeruginosa group; Pseudomonas aeruginosa

Package: MIGS: cultured bacteria/archaea, human-associated; version 6.0

Attributes

strain	PAK
collection date	Not collected
broad-scale environmental context	Not collected
local-scale environmental context	Not collected
environmental medium	Not collected
geographic location	Not collected
host	Homo sapiens
isolation and growth condition	Not collected
latitude and longitude	Not collected
number of replicons	Not collected
reference for biomaterial	Not collected

Description

Generating a comprehensive inventory of factors important for T3SS gene expression in Pseudomonas aeruginosa strain PAK. Transposon insertion libraries harboring T3SS fluorescent reporter were cultured under inducing conditions for T3SS gene expression and individual cells were sorted into T3SS-Off and T3SS-On populations on FACS. NexGen sequencing of the transposon insertion sites and comparison of the T3SS-Off and T3SS-On populations identified genes and small noncoding RNAs that playing a role in T3SS gene expression.

Keywords: GSC:MIxS;MIGS:6.0