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Protocols: Bacteria were grown in brain heart infusion (BHI) broth (BD) with shaking at 200 rpm at 37째C overnight. Bacterial suspensions were diluted in BHI broth and bacteria grown to OD 600nm=2 with shaking at 200 rpm at 37째C. Genomic DNA was isolated from bacteria using the PureLink DNeasy kit (Invitrogen) with the addition of 0.5 mg/mL (100mg/mL) lysozyme and 0.05 ng/mL (100-500ug/mL?) lysostaphin to the lysis buffer. Libraries were prepared using PacBio Barcoded Adapters for Multiplex SMRT Sequencing protocol (3.0 chemistry)
BioProject SRA
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