Warning: The NCBI web site requires JavaScript to function. more...
An official website of the United States government
The .gov means it's official. Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you're on a federal government site.
The site is secure. The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.
Protocols: Bacteria were harvested by centrifugation and the cell pellets were submerged in Trizol and shipped on dry ice to Macrogen Inc. (South Korea) for RNA extraction and sequencing. Samples were relabelled in a randomised order before sending to the sequencing provider, to avoid any potential for introduction of a technical batch effect based on which group (treated or untreated) the samples belonged to. Total RNA extraction was conducted by Macrogen Inc. (South Korea). Total RNA was quality checked to confirm that all were of acceptable RNA Integrity Number (RIN >7). Libraries were constructed using the Illumina TruSeq Stranded Total RNA (Illumina Inc, San Diego) protocol with ribosomal RNA depletion (Ribo-Zero).
SRA
Your browsing activity is empty.
Activity recording is turned off.
Turn recording back on