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F4S9A

Identifiers
BioSample: SAMEA2051878; SRA: ERS216923
Organism
Aspergillus fischeri
cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; Eurotiomycetes; Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus; Aspergillus subgen. Fumigati
Attributes
sample nameE-SYBR-1:F4S9A
strainF4S9A
ENA first public2016-04-30
ENA last update2013-02-22
External IdSAMEA2051878
INSDC center aliasEMBL European Bioinformatics Institute
INSDC center nameEMBL European Bioinformatics Institute
INSDC first public2016-04-30T17:09:58Z
INSDC last update2013-02-22T09:52:56Z
INSDC statuspublic
Submitter IdE-SYBR-1:F4S9A
bio source typefresh sample
broker nameArrayExpress
clinical historyenvironmental soil sample
itraconazole resistanceunknown resistance to itraconazole
overall azole resistanceunknown azole resistance
posaconazole resistanceunknown resistance to posaconazole
voraconazole resistanceunknown resistance to voraconazole to voraconazole
Description

Protocols: Genomic DNA was extracted using the CTAB genomic DNA extraction method. Libraries were constructed using Illumina TruSeq DNA Sample Prep standard protocol. Briefly, 5 ug of high molecular weight genomic DNA (gDNA) was fragmented by Covaris sonication device. Following sonication, DNA fragments were end-repaired and A-tailed. Adapters were then ligated via a 3' thymine overhang. Finally, ligated fragments were amplified by PCR (10 cycles). Insert sizes were 400-500 bp as evaluated in an Agilent DNA 1000 Analyzer Chip.

BioProject
PRJEB1497 Multiazole resistance in clinical and environmental fungal isolates
Retrieve all samples from this project

Submission
EBI; 2016-05-01
Accession:
SAMEA2051878
ID:
4922459

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