CEA10

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Organism: Aspergillus fumigatus
cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; Eurotiomycetes; Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus; Aspergillus subgen. Fumigati

Attributes

pathogenicity	pathogenic
sample name	E-SYBR-1:CEA10
strain	CEA10
ENA first public	2016-04-30
ENA last update	2013-02-22
External Id	SAMEA2052099
INSDC center alias	EMBL European Bioinformatics Institute
INSDC center name	EMBL European Bioinformatics Institute
INSDC first public	2016-04-30T17:09:58Z
INSDC last update	2013-02-22T09:52:57Z
INSDC status	public
Submitter Id	E-SYBR-1:CEA10
bio source type	fresh sample
broker name	ArrayExpress
clinical history	clinical sample
itraconazole resistance	sensitive to itraconazole
overall azole resistance	azole-sensitive
posaconazole resistance	sensitive to posaconazole
voraconazole resistance	sensitive to voraconazole

Description: Protocols: Genomic DNA was extracted using the CTAB genomic DNA extraction method. Libraries were constructed using Illumina TruSeq DNA Sample Prep standard protocol. Briefly, 5 ug of high molecular weight genomic DNA (gDNA) was fragmented by Covaris sonication device. Following sonication, DNA fragments were end-repaired and A-tailed. Adapters were then ligated via a 3' thymine overhang. Finally, ligated fragments were amplified by PCR (10 cycles). Insert sizes were 400-500 bp as evaluated in an Agilent DNA 1000 Analyzer Chip.