| broad-scale environmental context | aquatic biome |
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| collected by | JOHANA ROTTEROVA |
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| collection date | 2021-10-07 |
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| depth | 1899-12-30 |
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| environmental medium | ciliate culture |
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| geographic location | USA |
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| host life stage | vegetative cell |
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| host | Metopus strain FRESH26 |
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| host taxonomy ID | 2980496 (2980496) |
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| identified by | JOHANA ROTTEROVA |
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| investigation type | metagenome-assembled genome |
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| isolation source | Ciliate: Metopus strain FRESH26 |
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| tissue | WHOLE ORGANISM |
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| project name | ASG |
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| sample collection device or method | hand collected into a 50 ml tube |
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| sample name | ac794f53-cb0e-4bbe-a7ce-f3218f4774c6 |
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| specimen voucher | NOT PROVIDED |
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| 16S recovered | Yes |
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| 16S recovery software | PROKKA (version 1.14.5) |
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| ENA-CHECKLIST | ERC000047 |
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| GAL | University of British Columbia |
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| GAL_sample_id | NOT PROVIDED |
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| INSDC center name | Tree of Life Programme |
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| MAG coverage software | Minimap2 (version 2.24-r1122) |
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| Submitter Id | ac794f53-cb0e-4bbe-a7ce-f3218f4774c6 |
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| assembly quality | Single contiguous sequence without gaps or ambiguities with a consensus error rate equivalent to Q50 or better |
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| assembly software | metaMDBG |
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| barcoding center | UNIVERSITY OF RHODE ISLAND |
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| binning parameters | coverage; graph; hic-mapping |
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| binning software | MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR |
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| collecting institution | UNIVERSITY OF RHODE ISLAND |
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| completeness score | 99.67 |
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| completeness software | checkM (version 1.2.1); checkM_DB (release 2015-01-16) |
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| contamination score | 0.65 |
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| culture_or_strain_id | FRESH26 strain in culture collection of Roxanne Beinart, GSO, URI, RI, USA |
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| geographic location (latitude) | 41.49 |
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| geographic location (longitude) | -71.42 |
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| geographic location (region and locality) | RHODE ISLAND | NARRAGANSETT | UNIVERSITY OF RHODE ISLAND |
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| habitat | salt marsh sediment |
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| identifier_affiliation | UNIVERSITY OF RHODE ISLAND |
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| local environmental context | marine salt marsh biome |
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| metagenomic source | ciliate metagenome |
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| number of standard tRNAs extracted | 49 |
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| original collection date | 2019-08-22 |
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| original collection location | USA | MASSACHUSETTS | FALMOUTH | FRESH RIVER |
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| original geographic location (latitude) | 41.541917 |
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| original geographic location (longitude) | -70.622194 |
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| sample derived from | SAMEA114406700 |
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| scientific_name | uncultured Methanolobus sp. |
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| sequencing method | Pacbio Sequel II |
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| specimen_id | UOBC0000027 |
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| tRNA extraction software | PROKKA (version 1.14.5) |
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| taxonomic classification | GTDB-TK (version 2.1.1); GTDB (release 207_v2) |
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| taxonomic identity marker | taxonomic classification defined by topology and ANI |
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| tolid | piMetSpea1.Methanolobus_sp_1 |
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