| broad-scale environmental context | aquatic biome |
|---|
| collected by | JOHANA ROTTEROVA |
|---|
| collection date | 2021-10-07 |
|---|
| depth | 1899-12-30 |
|---|
| environmental medium | ciliate culture |
|---|
| geographic location | USA |
|---|
| host life stage | vegetative cell |
|---|
| host | Metopus strain FRESH26 |
|---|
| host taxonomy ID | 2980496 (2980496) |
|---|
| identified by | JOHANA ROTTEROVA |
|---|
| investigation type | metagenome-assembled genome |
|---|
| isolation source | Ciliate: Metopus strain FRESH26 |
|---|
| tissue | WHOLE ORGANISM |
|---|
| project name | ASG |
|---|
| sample collection device or method | hand collected into a 50 ml tube |
|---|
| sample name | 414527d9-51fc-44d8-9f9c-d43466d59f4e |
|---|
| specimen voucher | NOT PROVIDED |
|---|
| 16S recovered | Yes |
|---|
| 16S recovery software | PROKKA (version 1.14.5) |
|---|
| ENA-CHECKLIST | ERC000047 |
|---|
| GAL | University of British Columbia |
|---|
| GAL_sample_id | NOT PROVIDED |
|---|
| INSDC center name | Tree of Life Programme |
|---|
| MAG coverage software | Minimap2 (version 2.24-r1122) |
|---|
| Submitter Id | 414527d9-51fc-44d8-9f9c-d43466d59f4e |
|---|
| assembly quality | Single contiguous sequence without gaps or ambiguities with a consensus error rate equivalent to Q50 or better |
|---|
| assembly software | metaMDBG |
|---|
| barcoding center | UNIVERSITY OF RHODE ISLAND |
|---|
| binning parameters | coverage; graph; hic-mapping |
|---|
| binning software | MAGScoT (version 1.0.0); MetaBat (version 2.15-15-gd6ea400); bin3C (version 0.3.3); MaxBin (version 2.7); MetaTOR |
|---|
| collecting institution | UNIVERSITY OF RHODE ISLAND |
|---|
| completeness score | 99.93 |
|---|
| completeness software | checkM (version 1.2.1); checkM_DB (release 2015-01-16) |
|---|
| contamination score | 0.62 |
|---|
| culture_or_strain_id | FRESH26 strain in culture collection of Roxanne Beinart, GSO, URI, RI, USA |
|---|
| geographic location (latitude) | 41.49 |
|---|
| geographic location (longitude) | -71.42 |
|---|
| geographic location (region and locality) | RHODE ISLAND | NARRAGANSETT | UNIVERSITY OF RHODE ISLAND |
|---|
| habitat | salt marsh sediment |
|---|
| identifier_affiliation | UNIVERSITY OF RHODE ISLAND |
|---|
| local environmental context | marine salt marsh biome |
|---|
| metagenomic source | ciliate metagenome |
|---|
| number of standard tRNAs extracted | 108 |
|---|
| original collection date | 2019-08-22 |
|---|
| original collection location | USA | MASSACHUSETTS | FALMOUTH | FRESH RIVER |
|---|
| original geographic location (latitude) | 41.541917 |
|---|
| original geographic location (longitude) | -70.622194 |
|---|
| sample derived from | SAMEA114406700 |
|---|
| scientific_name | Vibrio fluvialis |
|---|
| sequencing method | Pacbio Sequel II |
|---|
| specimen_id | UOBC0000027 |
|---|
| tRNA extraction software | PROKKA (version 1.14.5) |
|---|
| taxonomic classification | GTDB-TK (version 2.1.1); GTDB (release 207_v2) |
|---|
| taxonomic identity marker | ani_screen |
|---|
| tolid | piMetSpea1.Vibrio_fluvialis_1 |
|---|