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Metagenome-assembled genome: ERR1278105_bin.101_CONCOCT_v1.1_MAG

Identifiers
BioSample: SAMEA14080525; SRA: ERS11683781
Organism
uncultured Porphyromonadaceae bacterium
cellular organisms; Bacteria; FCB group; Bacteroidota/Chlorobiota group; Bacteroidota; Bacteroidia; Bacteroidales; Porphyromonadaceae; environmental samples
Attributes
collection date2011-10-01
broad-scale environmental contextHost-associated
local-scale environmental contextMammals
environmental mediumDigestive system
geographic locationSweden
investigation typemetagenome-assembled genome
isolation sourcegut metagenome
project nameThe moose (Alces alces) manages to utilize energy from fiber-rich lignocellulose material through enzymes produced by the microbes in its rumen. Some of these specialized enzymes may outperform cellulases and hemicellulases that are used in bio-refinery processes today. Shotgun metagenomics offers a method to study microbial communities in detail and to capture the gene sequences coding for enzymes that can be produced in the laboratory. We applied the technique on rumen material from moose. Through a binning approach, a large number of metagenome-assembled genomes (MAGs) from single species and strains were isolated and characterized based on taxonomy and carbohydrate active enzyme (CAZY) profile. Most of the 99 MAGs were classified to the bacterial phyla Bacteriodetes and Firmicutes, which is in agreement with previous studies of rumen from moose and other herbivores. Phylogenomic analysis of MAGs revealed novel clades within Bacteriodetes with no related previously characterized genome. In addition, new genomes that were closely related to the recently described Melainabacteria, newly proposed candidate divisions SR1 and TM7 and previously uncharacterized Alphaproteobacteria were also retrieved. Putative cellulolytic functions were observed in several clades of Bacteroidetes and for MAGs of genus Ruminococcus, Fibrobacteres, and Treponema. Cellulosome components were present in MAGs of Ruminococcus but also in certain clades of Bacteroidetes. Cellulosomes are generally described in the Firmicutes family Clostridiales. Multiple putative cellulases were identified within species from several phyla. At a general level, the most frequently occurring polysaccharide-degrading enzymes targeted hemicellulose, starch and pectin. This study describes the first shotgun-metagenomic characterization of moose rumen and the high number of reconstructed genomes provides a valuable link between phylogeny and cellulolytic role.
sample nameERR1278105_bin.101_CONCOCT_v1.1_MAG
ENA-CHECKLISTERC000047
ENA-FIRST-PUBLIC2023-01-03
ENA-LAST-UPDATE2023-01-03
External IdSAMEA14080525
INSDC center aliasEBI
INSDC center nameEuropean Bioinformatics Institute
INSDC first public2023-01-03T00:32:55Z
INSDC last update2023-01-03T00:32:55Z
INSDC statuspublic
Submitter IdERR1278105_bin.101_CONCOCT_v1.1_MAG
assembly qualityMany fragments with little to no review of assembly other than reporting of standard assembly statistics
assembly softwarespadesv3.11.1
binning parametersDefault
binning softwareCONCOCT v1.1
broker nameEMG broker account, EMBL-EBI
completeness score80.62
completeness softwareCheckM
contamination score3.05
geographic location (latitude)56.752724
geographic location (longitude)16.688232
metagenomic sourcegut metagenome
sample derived fromSAMEA3878208
scientific_nameuncultured Porphyromonadaceae bacterium
sequencing methodIllumina HiSeq 2500
taxonomic identity markermulti-marker approach
Description

This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run ERR1278105 of study ERP014306.

BioProject
PRJEB51895 Large-scale analysis of novel cellular microbes from the mammal gut biome
Retrieve all samples from this project

Submission
EBI; 2023-01-04
Accession:
SAMEA14080525
ID:
32558213

BioProject Nucleotide

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