U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

Metagenome-assembled genome: SRR1628696_bin.5_MetaBAT_v2.12.1_MAG

Identifiers
BioSample: SAMEA9694624; SRA: ERS7418870
Organism
uncultured Firmicutes bacterium
cellular organisms; Bacteria; Bacillati; Bacillota; environmental samples
Attributes
collection date2008-08-10
broad-scale environmental contextaquatic
local-scale environmental contextmarine
environmental mediumwater
geographic locationPacific Ocean
investigation typemetagenome-assembled genome
isolation sourcemarine metagenome
project nameTo determine the role of mineralogy and composition on the microbial ecology of the deep igneous marine biosphere, we incubated twelve igneous minerals and glasses in IODP Hole 1301A on the eastern flank of the Juan de Fuca Ridge (47° 45.210′ N, 127° 45.833′ W) for four years (2004 - 2008) (Smith et al., 2011). Hole 1301A is a subseafloor microbial observatory equipped with a Circulation Obviation Retrofit Kit (CORK) that allowed us to suspend microbial flow cells 262 m within the basalt basement, exposing common igneous minerals and glasses to the aquifer community. Pyrotag sequencing revealed that the mineral olivine was dominated by the uncultured, and thus uncharacterized, archaeum Archaeoglobaceae OTU 5. The low complexity of the microbial community associated with olivine makes this an ideal candidate for shotgun metagenomic sequencing, with the explicit goal of reconstructing the metagenomes of members of the Archaeoglobaceae. Given that olivine is one of the most abundant minerals in oceanic crust, understanding the functional role of the uncultured Archaeoglobaceae in biogeochemical cycling in the marine subsurface is imperative and has the potential to provide transformative knowledge regarding mineral weathering on a global scale. We predict that Archaeoglobaceae OTU 5 is using either an iron-based or hydrogen-based metabolism on olivine surfaces, and it may be a major primary producer in olivine-rich crust.
sample nameSRR1628696_bin.5_MetaBAT_v2.12.1_MAG
ENA first public2021-09-16
ENA last update2021-09-16
ENA-CHECKLISTERC000047
External IdSAMEA9694624
INSDC center aliasEMG
INSDC center nameEMG
INSDC first public2021-09-16T16:25:22Z
INSDC last update2021-09-16T16:25:22Z
INSDC statuspublic
Submitter IdSRR1628696_bin.5_MetaBAT_v2.12.1_MAG
assembly qualityMany fragments with little to no review of assembly other than reporting of standard assembly statistics
assembly softwaremetaspadesv3.11.1
binning parametersDefault
binning softwareMetaBAT v2.12.1
broker nameEMG broker account, EMBL-EBI
completeness score88.28
completeness softwareCheckM
contamination score0.5
geographic location (latitude)47.7535
geographic location (longitude)-127.763885
metagenomic sourcemarine metagenome
sample derived fromSAMN03142812
sequencing methodIllumina HiSeq 1000
taxonomic identity markermulti-marker approach
Description

This sample represents a Third Party Annotation (TPA) Metagenome-Assembled Genome (MAG) assembled from the metagenomic run SRR1628696 of study SRP049252.

BioProject
PRJEB37807 Uncultured novel prokaryotic genomes associated with with aquatic environments
Retrieve all samples from this project

Submission
EBI; 2021-09-25
Accession:
SAMEA9694624
ID:
21733633

BioProject Nucleotide

Supplemental Content

Related information

Recent activity

Clear Turn Off Turn On

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...
You are here: NCBI > DNA & RNA > BioSample
Support Center