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Human fecal metatranscriptome from 10 mM sulfoquinovose-amended microcosm after 6 h incubation replicate 3

Identifiers
BioSample: SAMN14943932; Sample name: Human fecal metatranscriptome from 10 mM sulfoquinovose-amended microcosm after 6 h incubation replicate 3; SRA: SRS6673414
Organism
human gut metagenome
unclassified entries; unclassified sequences; metagenomes; organismal metagenomes
Package
Metagenome or environmental; version 1.0
Attributes
hostHomo sapiens
isolation source10 mM sulfoquinovose-amended microcosm after 6 h incubation replicate 3
collection date2016-11-01
geographic locationAustria: Vienna
latitude and longitude48.2084 N 16.3735 E
relationship to oxygenanaerobe
sample collection device or methodMicrocosm (Hungate tubes crimp-sealed with a rubber stopper) was subsampled using an 1 ml sterile syringe and 20G needle
sample material processingSubsampled fecal biomass was pelleted by centrifugation and pellet was stored at -80C. RNA was harvested by phenol/chloroform extraction followed by Dnase treatment.
sample size1
Description

Microcosms were constructed using homogenized vegetarian feces from 8 individuals. Microcosm was amended with 10 mM sulfoquinovose and incubated at 37C under anoxic conditions. This metatranscriptome was generated from RNA extracted from biomass, which was harvested 6 h post substrate addition. Microcosm replicate 3

BioProject
PRJNA593787 human gut metagenome
Retrieve all samples from this project

Submission
University of Vienna, Buck Hanson; 2020-05-18
Accession:
SAMN14943932
ID:
14943932

BioProject SRA

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