Mannheimia haemolytica USDA-ARS-184 Phase Variant ... - BioSample

Mannheimia haemolytica USDA-ARS-184 Phase Variant 20180323.H

Identifiers: BioSample: SAMN41523186; Sample name: M. haemolytica phase variant Mh184.20180323.PV.H

Organism: Mannheimia haemolytica
cellular organisms; Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Pasteurellales; Pasteurellaceae; Mannheimia

Package: MIGS: cultured bacteria/archaea; version 6.0

Attributes

strain	Mh184.20180323.PV.H
collection date	2018-03-23
broad-scale environmental context	ENVO:00002123
local-scale environmental context	ENVO:00002124
environmental medium	MICRO:0000067
geographic location	USA: Clay Center, NE
isolation and growth condition	5955799
latitude and longitude	NA
number of replicons	1
reference for biomaterial	NA
collection method	chemically defined minimal medium culture of SMOOTH colony sampleID 100198
encoded traits	SMOOTH phenotype
estimated size	2500000
host	Bos taurus
host disease	Bovine Respiratory Disease Complex
host taxonomy ID	9913 (9913)
isolation source	Derived from an anaerobic bioreactor culture in chemically defined minimal medium (CDMM) inoculated with a CDMM suspension of both SMOOTH and NONSMOOTH colonies grown on blood agar base + hemin plate (BABH) medium. This inoculating CDMM suspension assigned sampleID 100158. These BABH colonies were generated from a both SMOOTH and NONSMOOTH colonies on chocolate agar inoculated from a glycerol stock with sampleID 184 (SAMN03275447)
pathogenicity	Bos taurus
relationship to oxygen	facultative
source material identifiers	100198
subspecific genetic lineage	phase variant
ancestor_biosample	SAMN03275447

Description

A suspension of both SMOOTH and NONSMOOTH colonies (sample ID 100158) was used to inoculate a 72 hour anaerobic culture in chemically defined minmal medium in bioreactor vessel 4. This culture was diluted and plated on blood agar base + hemin medium and grown overnight at 37C under 5% CO2 to generate a SMOOTH colony with source material sampleID 100198. This colony was aerobically cultured overnight in chemically defined minmal medium from which the DNA for sequencing was extracted.

Keywords: GSC:MIxS;MIGS:6.0

BioProject: PRJNA172854 Mannheimia haemolytica
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Submission: USDA-ARS-USMARC, Gregory Harhay; 2024-05-24

Accession:: SAMN41523186
ID:: 41523186

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Mannheimia haemolytica USDA-ARS-184 Phase Variant 20180323.H

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